CN105309183A - Quick breeding method for rhizoma panacis majoris - Google Patents
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- CN105309183A CN105309183A CN201510662339.2A CN201510662339A CN105309183A CN 105309183 A CN105309183 A CN 105309183A CN 201510662339 A CN201510662339 A CN 201510662339A CN 105309183 A CN105309183 A CN 105309183A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention discloses a quick breeding method for rhizoma panacis majoris, the method comprising following steps: (1) ripe and healthy fruits of rhizoma panacis majoris collected in the current year are selected, then fruit flesh is rubbed and washed off to obtain seeds of rhizoma panacis majoris; (2) the cleaned seeds of rhizoma panacis majoris are dried in shade and sown in the current year; (3) the seeds are well stirred and mixed with substrate, then spread in a sowing basket, and the sowing basket is put in a water tank which contains water so that water permeates reversely to totally infiltrate the whole basket of sowing substrate, then the basket is taken out of the water and drained off water; (4) the sowing basket is put and cultivated in a thermostat cultivating chamber, and the cultivating temperature is 20+/-1 DEG C; the substrate should be watered regularly to keep moisture; (5) germinated seedlings are transplanted to a seedbed, shade net with light transmittance of 60% is selected to provide shade for cultivating, and the humidity of the substrate is kept at about 70% until the seedlings are survived. Rhizoma panacis majoris bred by the method takes about 150 days for germination, 180 days for seedling development, and the comprehensive seedling rate is more than 75%. Compared with traditional sowing and seedling method, seedling time is shortened by 120 to 150 days and seedling rate is increased by 45% and above by the quick breeding method.
Description
Technical field
The present invention relates to biological technical field, relate to a kind of method for quickly breeding of medicinal plant Panax Japonicus Var. Major particularly.
Background technology
Panax Japonicus Var. Major (Panaxjaponicusvar.major) is next mutation of panax japonicus kind under Araliaceae Panax, is that " Chinese Pharmacopoeia " and " Yunnan Province's drug standards " record kind, main product Yunnan.Panax Japonicus Var. Major is broad-spectrum traditional Chinese medicine, for the nationalitys such as Bai nationality, Naxi, Lisu, Tibetan commonly use, is that the preparation about more than 200 of raw material is planted with Panax Japonicus Var. Major.Since the eighties in 20th century, Kunming Institute of Zoology carried out medicinal plants study, large quantity research mainly concentrates on the aspects such as medicine resource classification and system, geographical distribution and resource assessment, chemical composition and pharmacologically active, reproductive biology, genetic diversity and introduction and acclimatization cultivation.Current artificial planting Panax Japonicus Var. Major is mainly by excavating wild resource as provenance and propagating materials, because the provenance place of production and growth year are different, provenance mixes, lacks and carry out system identification evaluation to resource in addition, especially be almost blank in Traits and chemical composition Regulation Mechanism and genetic development research, thus cause Panax Japonicus Var. Major breeding extended stationary periods in the introduction and acclimatization primary stage.Medicinal scope constantly expands in recent years, and demand increases year by year, and existing medicine source far can not ensure market demand.Because Panax Japonicus Var. Major reproduction rate is low, poor growth, only can not meet suitability for industrialized production, main producing region unauthorized and excessive mining in addition by wild resource, and ignore the protection of Panax Japonicus Var. Major resource and breed, Wild ornamental resources is seriously damaged.The self-sow speed of traditional medicinal part rhizome of Panax Japonicus Var. Major is very slow, the biomass that can not show a candle to ginseng is large, be medicinal commodity from seed growth, generally need 3-4, Panax Japonicus Var. Major resource regeneration is comparatively slow, and excavating in a large number year by year makes wild Panax Japonicus Var. Major resource increasingly exhausted, thus brings out Panax Japonicus Var. Major price and constantly rise, current Panax Japonicus Var. Major scarcity of resources, imbalance between supply and demand is given prominence to.Therefore, carry out the Fast-propagation of Panax Japonicus Var. Major, improving reproductive efficiency by artificial method provides seedling guarantee for planting, and in a planned way developing the plant husbandry of Panax Japonicus Var. Major, is the unique feasible approach guaranteeing Panax Japonicus Var. Major MED SUP.
So far, the technology of Panax Japonicus Var. Major propagation method is related in prior art, focus mostly in Panax Japonicus Var. Major tissue cultures and Wild ornamental resources introduction and acclimatization aspect, the Germination And Seedling cycle at existing Panax Japonicus Var. Major medicinal material planting base and plantation family mostly is more than 10 months, some areas even reach 14-16 month, germination rate is less than 50%, and the present invention is all better than prior art on sprout time and the performance of germination rate result.
Summary of the invention
The invention provides a kind of method for quickly breeding of medicinal plant Panax Japonicus Var. Major.
The technical solution used in the present invention is:
A kind of Panax Japonicus Var. Major method for quickly breeding, comprises the following steps:
(1) select maturation, the healthy Panax Japonicus Var. Major fruit of gathering then, removing pulp through rubbing rinsing, obtaining Panax Japonicus Var. Major seed;
(2) by clean after Panax Japonicus Var. Major seed dry in the shade, in sowing then;
(3) after seed and matrix being mixed thoroughly, spread in the sowing basket of the matrix of 5cm at the bottom of being evenly sprinkled upon basket, upper covering one deck is no more than the matrix of 1cm, ensure that seed does not expose in atmosphere, sowing basket is put into the tank that is filled with water, make moisture inverse osmosis until infiltrate whole basket sowing media, then take out control water;
(4) sowing basket is put into the indoor cultivation of constant temperature culture, temperature is 20 ± 1 DEG C; Matrix should regularly be watered moisturizing, and humid control is 30% ~ 40%, and after 150 ~ 180 days, germination rate can reach more than 80%;
(5) by the little transplantation of seedlings after sprouting on seedbed, select 60% light transmittance shade net to shade plantation, control soil humidity is about 70%, survives to seedling.
As preferred technical scheme, described matrix is that mountain base soil, peat composed of rotten mosses are native, one or more in fine sand.
As further preferred technical scheme, described matrix is mountain base soil, peat composed of rotten mosses is native, the fine sand mixed-matrix that mixes of 1:1:1 by volume.
As preferred technical scheme, mountain base soil and peat composed of rotten mosses is native pulverized 60 mesh sieves in described base starting material, fine sand cleans airing.
As preferred technical scheme, described step (5) medium and small transplantation of seedlings to seeding row spacing during seedbed is 10cm × 10cm.
Beneficial effect of the present invention:
(1) relative to prior art; although Panax Japonicus Var. Major seed germination problem can be solved from different perspectives; but the sprouting cycle is longer relatively; germination rate is not high; the average sprouting cycle, the technology of the present invention effectively can shorten the Panax Japonicus Var. Major seed germination time on prior art basis, within 150 ~ 180 days, can reach more than 80% germination rate all higher than 300 days; greatly reduce time cost for production and resource extentization utilize, improve efficiency.
(2) in conventional method, the Sha Zangyi that survives the winter causes seed dormancy, affects regularity and the uniformity of Second Year seed germination, and seedling of the present invention shades after sprouting in time, keeps soil humidity, improves kind of a shoot survival percent.
(3) Panax Japonicus Var. Major method for quickly breeding of the present invention; vital effect is served to the reduction of this wild genunie medicinal materials reproductive-cost and the expansion of breeding scale; protect wild resource on this basis; prevent resource exhaustive exploitation, for its sustainable use and development provide material base, scientific basis and technical support.
Embodiment
Below in conjunction with the specific embodiment of the invention, be clearly and completely described technical scheme of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
A kind of Panax Japonicus Var. Major method for quickly breeding, comprises the following steps:
(1) select maturation, the healthy Panax Japonicus Var. Major fruit of gathering then, removing pulp through rubbing rinsing, obtaining Panax Japonicus Var. Major seed;
(2) by clean after Panax Japonicus Var. Major seed dry in the shade, in sowing then;
(3) after seed and matrix being mixed thoroughly, spread in the sowing basket of the matrix of 5cm at the bottom of being evenly sprinkled upon basket, upper covering one deck is no more than the matrix of 1cm, ensure that seed does not expose in atmosphere, sowing basket is put into the tank that is filled with water, make moisture inverse osmosis until infiltrate whole basket sowing media, then take out control water; Matrix selects mountain base soil to pulverize 60 mesh sieves;
(4) sowing basket is put into the indoor cultivation of constant temperature culture, temperature is 20 ± 1 DEG C; Matrix should regularly be watered moisturizing, and humid control is 30% ~ 40%, and after 150 ~ 180 days, germination rate can reach more than 80%;
(5) by the little transplantation of seedlings after sprouting on seedbed, seeding row spacing is 10cm × 10cm, selects 60% light transmittance shade net to shade plantation, and control soil humidity is about 70%, and survive to seedling, comprehensive planting percent reaches more than 75%.
Embodiment 2
A kind of Panax Japonicus Var. Major method for quickly breeding, comprises the following steps:
(1) select maturation, the healthy Panax Japonicus Var. Major fruit of gathering then, removing pulp through rubbing rinsing, obtaining Panax Japonicus Var. Major seed;
(2) by clean after Panax Japonicus Var. Major seed dry in the shade, in sowing then;
(3) after seed and matrix being mixed thoroughly, spread in the sowing basket of the matrix of 5cm at the bottom of being evenly sprinkled upon basket, upper covering one deck is no more than the matrix of 1cm, ensure that seed does not expose in atmosphere, sowing basket is put into the tank that is filled with water, make moisture inverse osmosis until infiltrate whole basket sowing media, then take out control water; Matrix selects peat composed of rotten mosses soil to pulverize 60 mesh sieves;
(4) sowing basket is put into the indoor cultivation of constant temperature culture, temperature is 20 ± 1 DEG C; Matrix should regularly be watered moisturizing, and humid control is 30% ~ 40%, and after 150 ~ 180 days, germination rate can reach more than 80%;
(5) by the little transplantation of seedlings after sprouting on seedbed, seeding row spacing is 10cm × 10cm, selects 60% light transmittance shade net to shade plantation, and control soil humidity is about 70%, and survive to seedling, comprehensive planting percent reaches more than 75%.
Embodiment 3
A kind of Panax Japonicus Var. Major method for quickly breeding, comprises the following steps:
(1) select maturation, the healthy Panax Japonicus Var. Major fruit of gathering then, removing pulp through rubbing rinsing, obtaining Panax Japonicus Var. Major seed;
(2) by clean after Panax Japonicus Var. Major seed dry in the shade, in sowing then;
(3) after seed and matrix being mixed thoroughly, spread in the sowing basket of the matrix of 5cm at the bottom of being evenly sprinkled upon basket, upper covering one deck is no more than the matrix of 1cm, ensure that seed does not expose in atmosphere, sowing basket is put into the tank that is filled with water, make moisture inverse osmosis until infiltrate whole basket sowing media, then take out control water; Matrix cleans the fine sand of airing;
(4) sowing basket is put into the indoor cultivation of constant temperature culture, temperature is 20 ± 1 DEG C; Matrix should regularly be watered moisturizing, and humid control is 30% ~ 40%, and after 150 ~ 180 days, germination rate can reach more than 80%;
(5) by the little transplantation of seedlings after sprouting on seedbed, seeding row spacing is 10cm × 10cm, selects 60% light transmittance shade net to shade plantation, and control soil humidity is about 70%, and survive to seedling, comprehensive planting percent reaches more than 75%.
Embodiment 4
A kind of Panax Japonicus Var. Major method for quickly breeding, comprises the following steps:
(1) select maturation, the healthy Panax Japonicus Var. Major fruit of gathering then, removing pulp through rubbing rinsing, obtaining Panax Japonicus Var. Major seed;
(2) by clean after Panax Japonicus Var. Major seed dry in the shade, in sowing then;
(3) after seed and matrix being mixed thoroughly, spread in the sowing basket of the matrix of 5cm at the bottom of being evenly sprinkled upon basket, upper covering one deck is no more than the matrix of 1cm, ensure that seed does not expose in atmosphere, sowing basket is put into the tank that is filled with water, make moisture inverse osmosis until infiltrate whole basket sowing media, then take out control water; The mixed-matrix that matrix selects mountain base soil, the native 1:1 by volume of peat composed of rotten mosses mixes, mountain base is native and peat composed of rotten mosses is native pulverized 60 mesh sieves;
(4) sowing basket is put into the indoor cultivation of constant temperature culture, temperature is 20 ± 1 DEG C; Matrix should regularly be watered moisturizing, and humid control is 30% ~ 40%, and after 150 ~ 180 days, germination rate can reach more than 80%;
(5) by the little transplantation of seedlings after sprouting on seedbed, seeding row spacing is 10cm × 10cm, selects 60% light transmittance shade net to shade plantation, and control soil humidity is about 70%, and survive to seedling, comprehensive planting percent reaches more than 75%.
Embodiment 5
A kind of Panax Japonicus Var. Major method for quickly breeding, comprises the following steps:
(1) select maturation, the healthy Panax Japonicus Var. Major fruit of gathering then, removing pulp through rubbing rinsing, obtaining Panax Japonicus Var. Major seed;
(2) by clean after Panax Japonicus Var. Major seed dry in the shade, in sowing then;
(3) after seed and matrix being mixed thoroughly, spread in the sowing basket of the matrix of 5cm at the bottom of being evenly sprinkled upon basket, upper covering one deck is no more than the matrix of 1cm, ensure that seed does not expose in atmosphere, sowing basket is put into the tank that is filled with water, make moisture inverse osmosis until infiltrate whole basket sowing media, then take out control water; Matrix selects peat composed of rotten mosses soil, the fine sand mixed-matrix that mixes of 1:1 by volume, and peat composed of rotten mosses soil pulverized 60 mesh sieves, and fine sand cleans airing;
(4) sowing basket is put into the indoor cultivation of constant temperature culture, temperature is 20 ± 1 DEG C; Matrix should regularly be watered moisturizing, and humid control is 30% ~ 40%, and after 150 ~ 180 days, germination rate can reach more than 80%;
(5) by the little transplantation of seedlings after sprouting on seedbed, seeding row spacing is 10cm × 10cm, selects 60% light transmittance shade net to shade plantation, and control soil humidity is about 70%, and survive to seedling, comprehensive planting percent reaches more than 75%.
Embodiment 6
A kind of Panax Japonicus Var. Major method for quickly breeding, comprises the following steps:
(1) select maturation, the healthy Panax Japonicus Var. Major fruit of gathering then, removing pulp through rubbing rinsing, obtaining Panax Japonicus Var. Major seed;
(2) by clean after Panax Japonicus Var. Major seed dry in the shade, in sowing then;
(3) after seed and matrix being mixed thoroughly, spread in the sowing basket of the matrix of 5cm at the bottom of being evenly sprinkled upon basket, upper covering one deck is no more than the matrix of 1cm, ensure that seed does not expose in atmosphere, sowing basket is put into the tank that is filled with water, make moisture inverse osmosis until infiltrate whole basket sowing media, then take out control water; Matrix selects mountain base soil, the fine sand mixed-matrix that mixes of 1:1 by volume, and mountain base soil pulverized 60 mesh sieves, and fine sand cleans airing;
(4) sowing basket is put into the indoor cultivation of constant temperature culture, temperature is 20 ± 1 DEG C; Matrix should regularly be watered moisturizing, and humid control is 30% ~ 40%, and after 150 ~ 180 days, germination rate can reach more than 80%;
(5) by the little transplantation of seedlings after sprouting on seedbed, seeding row spacing is 10cm × 10cm, selects 60% light transmittance shade net to shade plantation, and control soil humidity is about 70%, and survive to seedling, comprehensive planting percent reaches more than 75%.
Embodiment 7
A kind of Panax Japonicus Var. Major method for quickly breeding, comprises the following steps:
(1) select maturation, the healthy Panax Japonicus Var. Major fruit of gathering then, removing pulp through rubbing rinsing, obtaining Panax Japonicus Var. Major seed;
(2) by clean after Panax Japonicus Var. Major seed dry in the shade, in sowing then;
(3) after seed and matrix being mixed thoroughly, spread in the sowing basket of the matrix of 5cm at the bottom of being evenly sprinkled upon basket, upper covering one deck is no more than the matrix of 1cm, ensure that seed does not expose in atmosphere, sowing basket is put into the tank that is filled with water, make moisture inverse osmosis until infiltrate whole basket sowing media, then take out control water; Matrix selects that mountain base soil, peat composed of rotten mosses are native, the fine sand mixed-matrix that mixes of 1:1:1 by volume, mountain base soil and peat composed of rotten mosses is native pulverized 60 mesh sieves, and fine sand cleans airing;
(4) sowing basket is put into the indoor cultivation of constant temperature culture, temperature is 20 ± 1 DEG C; Matrix should regularly be watered moisturizing, and humid control is 30% ~ 40%, and after 150 ~ 180 days, germination rate can reach more than 80%;
(5) by the little transplantation of seedlings after sprouting on seedbed, seeding row spacing is 10cm × 10cm, selects 60% light transmittance shade net to shade plantation, and control soil humidity is about 70%, and survive to seedling, comprehensive planting percent reaches more than 75%.
The beneficial effect of the technology of the present invention is proved below by concrete test example:
Test example 1:
One, materials and methods
Tested in October ,-2014 in October, 2013, in Lijiang County In Yunnan Province city Zhi Cheng biotechnology development corporation, Ltd.'s medicinal material planting base, choose Panax Japonicus Var. Major seed that is wild and cultivating and growing and carry out sowing experiment.Choosing humus soil as sowing media, through rubbing rinsing Panax Japonicus Var. Major fruit, obtaining Panax Japonicus Var. Major seed, hide 6 months through fine sand moisturizing sand, keep humidity to be not less than 50%, in April, 2014 sowing to the large Tanaka to improvement matrix, above cover shade net and shade.Under field conditions (factors), Panax Japonicus Var. Major seed germination situation is observed.
Two, results and analysis
Under self seed condition, Panax Japonicus Var. Major seed germination rate is 50%, and before falling seedling in October, 2014, comprehensive planting percent is 30%.Mainly due to passing through sand storing, overwintering, part Panax Japonicus Var. Major seed enters dormancy, and to a certain degree reducing appears in germination rate; And seed is directly seeded in field, without sprouting process and hardening process, regularity and uniformity are not high, and seedling very easily causes death under mesoclimate changes violent situation, so comprehensive planting percent is also not high.
Test example 2:
One, materials and methods
Tested year August in October, 2013 to 2014, carry out in nursery, Kunming Inst. of Botany, Chinese Academy of Sciences's Lijing alpine garden and culturing room, choose that Panax Japonicus Var. Major seed that is wild and cultivating and growing carries out sowing, seedling growth test.Choose that matrix comprises that mountain base soil, peat composed of rotten mosses are native, fine sand etc., arrange 7 groups of tests (specifically in table one) according to different ratio, requiring that mountain base is native, peat composed of rotten mosses is native pulverizes and sieves, and fine sand cleans airing.Often organize matrix and sow 5 baskets, every basket of 100 seeds, sowing basket is of a size of: the wide * of long * is high=60cm*40cm*10cm, selecting maturation, the healthy Panax Japonicus Var. Major fruit of gathering then, removing pulp through rubbing rinsing, obtain Panax Japonicus Var. Major seed, done by shady by Panax Japonicus Var. Major seed after cleaning, sow, sowing basket puts into the indoor cultivation of constant temperature culture, temperature controls as 20-25 DEG C, and soil humidity controls at 30-40%.Through 5-6 month, will sprout seedling replanting on seedbed, and require that seeding row spacing will be 10cm*10cm, and select 60% light transmittance shade net to shade, controlling soil humidity is about 70%, and September, seedling fell before seedling extremely then, observation planting percent.
Two, results and analysis
Table one different substrates Panax Japonicus Var. Major sowing test result
As can be seen from table one, different sowing and hardening matrix is selected to have certain influence to Panax Japonicus Var. Major sprouting and seedling, can draw from result of the test, add the matrix of fine sand, seed germination rate is relatively high, fine sand serves permeable effect in Panax Japonicus Var. Major seed germination, prevents seed from sprouting and going rotten in plumule emergence; In hardening process, preferred substrate proportioning is mountain base soil: peat composed of rotten mosses soil: the mixed-matrix of fine sand=1:1:1, and mainly due to and mountain base soil native by peat composed of rotten mosses, seedling effectively can absorb nutrient, is convenient to survive.By this test, finally can reach Panax Japonicus Var. Major about 150 days sprouting, 180 days seedlings, comprehensive planting percent reaches more than 75%, compares tradition sowing and seedling-cultivating method, shortens Germination And Seedling time 120-150 days, improve planting percent more than 45%.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1. a Panax Japonicus Var. Major method for quickly breeding, is characterized in that, comprises the following steps:
(1) select maturation, the healthy Panax Japonicus Var. Major fruit of gathering then, removing pulp through rubbing rinsing, obtaining Panax Japonicus Var. Major seed;
(2) by clean after Panax Japonicus Var. Major seed dry in the shade, in sowing then;
(3) after seed and matrix being mixed thoroughly, spread in the sowing basket of the matrix of 5cm at the bottom of being evenly sprinkled upon basket, upper covering one deck is no more than the matrix of 1cm, ensure that seed does not expose in atmosphere, sowing basket is put into the tank that is filled with water, make moisture inverse osmosis until infiltrate whole basket sowing media, then take out control water;
(4) sowing basket is put into the indoor cultivation of constant temperature culture, temperature is 20 ± 1 DEG C; Matrix should regularly be watered moisturizing, and humid control is 30% ~ 40%, and after 150 ~ 180 days, germination rate can reach more than 80%;
(5) by the little transplantation of seedlings after sprouting on seedbed, select 60% light transmittance shade net to shade plantation, control soil humidity is about 70%, survives to seedling.
2. Panax Japonicus Var. Major method for quickly breeding according to claim 1, is characterized in that, described matrix is that mountain base soil, peat composed of rotten mosses are native, one or more in fine sand.
3. Panax Japonicus Var. Major method for quickly breeding according to claim 1, is characterized in that, described matrix is mountain base soil, peat composed of rotten mosses is native, the fine sand mixed-matrix that mixes of 1:1:1 by volume.
4. the Panax Japonicus Var. Major method for quickly breeding according to any one of claims 1 to 3, is characterized in that, mountain base soil and peat composed of rotten mosses is native pulverized 60 mesh sieves in described base starting material, fine sand cleans airing.
5. Panax Japonicus Var. Major method for quickly breeding according to claim 1, is characterized in that, described step (5) medium and small transplantation of seedlings to seeding row spacing during seedbed is 10cm × 10cm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105900723A (en) * | 2016-04-19 | 2016-08-31 | 丽江志成生物开发有限公司 | Panax japonicus var.major seedling method and culture method |
| CN106717829A (en) * | 2016-11-29 | 2017-05-31 | 丽江光华生物开发有限公司 | The implantation methods of Panax Japonicus Var. Major |
| CN106961955A (en) * | 2017-03-21 | 2017-07-21 | 凤冈县领益昕中药材种植有限公司 | Panax Japonicus Var. Major method for culturing seedlings |
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| CN104255255A (en) * | 2014-09-23 | 2015-01-07 | 维西傈山中药材经营有限公司 | Method for breeding rhizoma panacis majoris seedlings in high and cold areas |
| CN104663234A (en) * | 2015-03-23 | 2015-06-03 | 宣威市美睿农业开发有限责任公司 | Acclimation method of wild rhizoma panacis majoris |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105900723A (en) * | 2016-04-19 | 2016-08-31 | 丽江志成生物开发有限公司 | Panax japonicus var.major seedling method and culture method |
| CN105900723B (en) * | 2016-04-19 | 2019-09-06 | 丽江志成生物开发有限公司 | A kind of method for culturing seedlings and cultural method of panax japonicus majoris |
| CN106717829A (en) * | 2016-11-29 | 2017-05-31 | 丽江光华生物开发有限公司 | The implantation methods of Panax Japonicus Var. Major |
| CN106961955A (en) * | 2017-03-21 | 2017-07-21 | 凤冈县领益昕中药材种植有限公司 | Panax Japonicus Var. Major method for culturing seedlings |
| CN106961955B (en) * | 2017-03-21 | 2020-09-22 | 凤冈县领益昕中药材种植有限公司 | Method for culturing seedlings of panax japonicus |
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