CN101015251A - Method for treating autumn sowing seeds of bulbus fritillariae cirrhosae - Google Patents
Method for treating autumn sowing seeds of bulbus fritillariae cirrhosae Download PDFInfo
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- CN101015251A CN101015251A CN 200610172188 CN200610172188A CN101015251A CN 101015251 A CN101015251 A CN 101015251A CN 200610172188 CN200610172188 CN 200610172188 CN 200610172188 A CN200610172188 A CN 200610172188A CN 101015251 A CN101015251 A CN 101015251A
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- bulbus fritillariae
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- 238000000034 method Methods 0.000 title claims abstract description 45
- 238000009331 sowing Methods 0.000 title abstract description 11
- 239000002689 soil Substances 0.000 claims abstract description 56
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 27
- 239000003448 gibberellin Substances 0.000 claims abstract description 27
- 239000000463 material Substances 0.000 claims abstract description 6
- 238000002791 soaking Methods 0.000 claims abstract description 3
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 21
- 238000007654 immersion Methods 0.000 claims description 15
- 241001644295 Fritillaria przewalskii Species 0.000 claims description 13
- 238000010899 nucleation Methods 0.000 claims description 12
- 238000012545 processing Methods 0.000 claims description 11
- 239000003415 peat Substances 0.000 claims description 6
- 108010016634 Seed Storage Proteins Proteins 0.000 claims description 5
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 abstract description 6
- 238000001035 drying Methods 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 description 11
- 238000003860 storage Methods 0.000 description 8
- 241001547127 Fritillaria cirrhosa Species 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000035784 germination Effects 0.000 description 5
- 241000935235 Fritillaria meleagris Species 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 239000005648 plant growth regulator Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 2
- 244000037666 field crops Species 0.000 description 2
- 238000010413 gardening Methods 0.000 description 2
- 230000014284 seed dormancy process Effects 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 1
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 240000007175 Datura inoxia Species 0.000 description 1
- 241000605372 Fritillaria Species 0.000 description 1
- 241001644297 Fritillaria delavayi Species 0.000 description 1
- 241001547125 Fritillaria thunbergii Species 0.000 description 1
- 241001644290 Fritillaria unibracteata Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the field of medicinal material cultivation, and particularly relates to a method for treating autumn sowing seeds of bulbus fritillariae cirrhosae. The method comprises the following steps: a. selecting full seeds harvested in the current year, uniformly mixing the treated seeds with soil, and storing for 3-15 weeks under the conditions of light and humidity; b. taking out the seeds, and soaking the seeds in gibberellin solution with the concentration of 10-80 ppm; c. and drying the soaked seeds and sowing. The method can ensure that the emergence rate of the bulbus fritillariae cirrhosae in autumn sowing reaches more than 88 percent, the seedling protection rate in one year reaches more than 75 percent, and the seedlings grow robustly. The seed utilization rate is improved, the yield of the bulbus fritillariae cirrhosae is increased, and the method is very simple and convenient, is suitable for field popularization and has a good application prospect.
Description
Technical field
The invention belongs to the plant cultivation field, especially relate to a kind of method of handling fall seeding seed of Sichuan fritillary bulb.
Background technology
Bulbus Fritillariae Cirrhosae is the dry bulb of Liliaceae Liliaceae Fritillaria Fririllaria L. various plants.Be rare traditional Chinese medicine commonly used, bitter, sweet, cold nature, have clearing heat and moistening lung, preventing phlegm from forming and stopping coughing function, it is different from other bulb of fritillary class medicinal material with its unique curative effect, at home and abroad enjoys high reputation, and is one of birth canal ground, the most representative river traditional Chinese medicine.The Pharmacopoeia of the People's Republic of China is gone through version and is recorded the dry bulb that Bulbus Fritillariae Cirrhosae is liliaceous plant Bulbus Fritillariae Cirrhosae F.cirrhosa D.Don, dark violet bulb of fritillary Fritillaria unibracteata Hsiao et K.C.Hisa, Gansu bulb of fritillary Fritillaria przewalskii Maxim. or Bulbus Fritillariae cirrhosae Fritillaria delavayi Franch., and the plant of these several product Bulbus Fritillariae Cirrhosaes also is Bulbus Fritillariae Cirrhosae in this area, also all is called Bulbus Fritillariae Cirrhosae in the present invention.Bulbus Fritillariae Cirrhosae mainly is distributed in former shrubbery, the grassy marshland area of river Western Hills of 3200～4200 meters of height above sea level, belongs to Qinghai-Tibet southeast edge part.Because the Bulbus Fritillariae Cirrhosae commodity are mainly derived from wild plant resource, long-term excessive excavating, the Bulbus Fritillariae Cirrhosae that once abounds with in the high-quality Bulbus Fritillariae Cirrhosae zone is disappeared in flakes, and this not only directly has influence on the output and the quality of relevant Chinese patent medicine preparation, even its species have been constituted real threat.Protection Bulbus Fritillariae Cirrhosae resource, it has been instant important process that this traditional Chinese medicinal materials assortment in imminent danger is carried out the large-scale artificial cultivation and production.
Artificial cultivation to Bulbus Fritillariae Cirrhosae mainly is to carry out to carry out vegetative mode with its bulb at present, but this method cost height, technical difficulty is big, and is unfavorable for the maintenance of Bulbus Fritillariae Cirrhosae self varietal character.And use its seed to carry out that though sexual propagation is subjected to that its seed dormancy difficulty is broken, emergence rate is low, the neat seedling time is long, the growth of seedling situation is undesirable, the puzzlement of problem such as 1 year survival rate of seedlings is low, but still be the important development direction of the artificial cultivation technique of current Bulbus Fritillariae Cirrhosae.Fritillaria cirrhosa seeds gibberellin treatment research provides a kind of usefulness 20～40ppm to handle fritillaria cirrhosa seeds improving emergence rate and to shorten neat seedling time method in (1994 the 8th the 1st phases of volume of basic unit's Chinese medicine magazine), but this method also have the neat seedling time still long, the growth of seedling situation is undesirable, 1 year survival rate of seedlings is low etc., and problem exists.
Summary of the invention
Technical problem to be solved by this invention provides a kind of method of handling fall seeding seed of Sichuan fritillary bulb.This method may further comprise the steps: a, choose then the full seed of results, with the native mixing of seeds treated, place the lucifuge wet condition to store following 3-15 week; B, taking-up seed are that the 10-80ppm Gibberellins solution is soaked with concentration; C, the seed after will soaking dry, sowing.
Wherein, the seed storage condition among the said method step a is a temperature-4 ℃～12 ℃, humidity 15%～20%.Said temperature and damp condition all are temperature and humidity conditions of the soil layer that closely contacts with seed.
Further, the seed storage condition among the said method step a is for to be embedded in seed in the seed bed of dark 15-60cm, blinding 50cm～20cm on the Seed Layer.Further, cover one deck heat and moisture preserving material again on the soil layer that is covered on the Seed Layer.Using of heat and moisture preserving material is to be temperature-4 ℃～12 ℃, humidity 15%～20% in order to ensure the temperature and humidity condition of the soil layer that closely contacts with seed.
Wherein, the usage amount of the soil among the said method step a is 2～6 times of volumes of seed volume, and described soil is at least a in yellow soil, detritus soil or the peat soil.
Wherein, the Gibberellins solution concentration among the said method step b is 10ppm～80ppm.Preferred Gibberellins solution concentration is 20ppm～60ppm.
Wherein, the Gibberellins solution immersion treatment time among the said method step b is 12 hours～72 hours.The preferred Gibberellins solution immersion treatment time is 24 hours～48 hours.
Wherein, the seed density of sowing among the said method step c is 2000～4000/square metre.
Gibberellin treatment, seed storage processing, storage have significant effects with aspects such as soil selections to last effect in the inventive method, as long as method is proper, land for growing field crops seed germination rate, neat seedling time, 1 year indexs such as survival rate of seedlings all have higher level, and the growth of seedlings situation is better.
The step of gibberellin treatment is important.Experiment shows that simultaneously the former germination rate is high by about 15% by imbedding seed that seed bed handles after handling with the debita spissitudo plant growth regulator and testing without the autumn sowing that plant growth regulator is handled the seed of just imbedding seed bed.Improve the availability of seed greatly, and also there has been certain help the neat seedling time that shortens seedling simultaneously.
The inventive method is creatively handled fritillaria cirrhosa seeds gibberellin treatment and storage then the align shortening of seedling time, the raising of survival rate of seedlings, the healthy and strong degree of seedling of combining significant collaborative facilitation.When carrying out the processing of fritillaria cirrhosa seeds storage, seed is mixed with the fine earth processing of sieving, handle back-set bed in the rectangle seed treatment bed of dark 20cm, wide 40cm, seed lamination thickness is 10cm, blinding 10cm on the Seed Layer, institute's blinding layer can be smashed the back use with the common soil that digs the seed bed generation with soil on the Seed Layer, also can be to handle the used soil of seed, and the used soil of seed is handled in preferred use.The threshing seed nuzzle seed bed finish in the ground in warm form after-ripening and low temperature physiology after-ripening stage, the storage back of finishing dealing with is taken out and to be carried out growth regulator and handle, and then in normal autumn sowing time sowing.(fineness of used soil is the fineness commonly used of general gardening or field of planting in addition storage to be handled employed soil, be advisable mixing with seed) kind carry out preferably also obviously improving germination rate, can select for use in common plantation according to actual conditions with soil (being generally yellow soil), detritus soil or peat soil.In the inventive method, use different soil, its germination rate: common yellow soil＜detritus soil＜peat soil in the condition of equating.Wherein, peat soil on the plateau or marsh wetland content than horn of plenty, be a kind of content of organic matter height, the uniform high-quality soil of grain structure also can be bought in market at present and obtain.Be the preferred of the inventive method, use the effects such as raising germination rate, survival rate of seedlings and growth of seedling level that reached to be difficult to predict in the methods of the invention.
Problems such as beneficial effect of the present invention is: the inventive method has overcome the seed dormancy difficulty that the artificial cultivation method of existing Bulbus Fritillariae Cirrhosae plant exists to be broken, and emergence rate is low, and the neat seedling time is long, and the growth of seedling situation is undesirable, and 1 year survival rate of seedlings is low.The inventive method emergence rate can reach more than 86%, and survival rate of seedlings can reach more than 75% in 1 year, the growth of seedling stalwartness, and resultant effect is better than prior art greatly.The inventive method has improved the seed availability greatly, has increased Bulbus Fritillariae Cirrhosae output, and method is very easy, is suitable for the land for growing field crops and promotes, and has fabulous application prospect.
Below by detailed description, illustrate but do not limit the present invention better embodiment of the present invention.
Embodiment
Embodiment one usefulness the inventive method is handled fritillaria cirrhosa seeds
This experiment is chosen dark violet fritillaria thunbergii as experimental subjects, and the gibberellin of use and various soil are common commercially available.
The seed of A for handling by the inventive method.
A1: get 2000 pieces of seeds that health is full at random, be embedded in the seed bed behind the yellow soil mixing with 2 times of seed volumes, the soil that loam cake 10cm is thick takes out in 3 week backs, drains after 72 hours with the Gibberellins solution immersion treatment of 10ppm.
A2: get 2000 pieces of seeds that health is full at random, be embedded in the seed bed behind the yellow soil mixing with 5 times of seed volumes, the soil that loam cake 15cm is thick takes out in 6 week backs, drains after 48 hours with the Gibberellins solution immersion treatment of 20ppm.
A3: get 2000 pieces of seeds that health is full at random, be embedded in the seed bed behind the yellow soil mixing with 5 times of seed volumes, the soil that loam cake 15cm is thick takes out in 6 week backs, drains after 48 hours with the Gibberellins solution immersion treatment of 30ppm.
A31: get 2000 pieces of seeds that health is full at random, be embedded in the seed bed behind the detritus soil mixing with 5 times of seed volumes, the soil that loam cake 15cm is thick takes out in 6 week backs, drains after 48 hours with the Gibberellins solution immersion treatment of 30ppm.
A32: get 2000 pieces of seeds that health is full at random, be embedded in the seed bed behind the peat soil mixing with 5 times of seed volumes, the soil that loam cake 15cm is thick takes out in 5 week backs, drains after 48 hours with the Gibberellins solution immersion treatment of 30ppm.
A4: get 2000 pieces of seeds that health is full at random, be embedded in the seed bed behind the yellow soil mixing with 4 times of seed volumes, the fine earth that loam cake 20cm is thick takes out in 8 week backs, drains after 32 hours with the Gibberellins solution immersion treatment of 40ppm.
A5: get 2000 pieces of seeds that health is full at random, be embedded in the seed bed behind the yellow soil mixing with 3 times of seed volumes, the soil that loam cake 10cm is thick takes out in 10 week backs, drains after 24 hours with the Gibberellins solution immersion treatment of 60ppm.
A6: get 2000 pieces of seeds that health is full at random, be embedded in the seed bed behind the yellow soil mixing with 6 times of seed volumes, the soil that loam cake 15cm is thick is in the back taking-up of 15 weeks.Gibberellins solution immersion treatment with 80pmm drained after 12 hours.
B imbeds 2000 pieces in the seed that seed bed stored for 6 weeks for just handling without plant growth regulator with the detritus soil mixing of 5 times of seed volumes.
C is for storing with imbedding seed bed, 2000 pieces in the seed that drains after 32 hours with the Gibberellins solution immersion treatment of 20ppm.
D is for storing with imbedding seed bed, 2000 pieces in the seed that drains after 32 hours with the Gibberellins solution immersion treatment of 30ppm.
E is for storing with imbedding seed bed, 2000 pieces in the seed that drains after 32 hours with the Gibberellins solution immersion treatment of 40ppm.
F handles without plant growth regulator, does not also imbed 2000 pieces in the seed of seed bed storage.
It is in the temperature-4 ℃～12 ℃, in the humidity 15%～20% that the storage condition of each group of wherein handling in seed bed all keeps the temperature and humidity condition of the soil layer that closely contacts with seed, institute's blinding layer can be smashed the back use with the common soil that digs the seed bed generation with soil on the Seed Layer, also can be to handle the used soil of seed, all use in each group of present embodiment and handle the used soil of seed, the fineness of used soil is the fineness commonly used of general gardening or field of planting, is advisable mixing with seed.
Each organizes the seed natural seasoning to essentially identical degree, admix ash respectively and be mixed into the seed ash with the fine earth that sieves, at the close height above sea level in aspects such as fertility, flatness is autumn sowing time sowing respectively simultaneously routinely in the level land about 3500 meters, blinding 2-5cm after planting, paste plantation cover sunshade net again, treat that lifting sunshade net height degree is 30～40cm behind the neat seedling of the bulb of fritillary, each group is all carried out same field management simultaneously.Write down whether index such as stalwartness of emergence rate (%), neat seedling time, 1 year survival rate of seedlings (%), seedling outward appearance in experimentation, experimental result sees Table 1.
Table 1 the inventive method is handled the fritillaria cirrhosa seeds experimental result
| Germination rate (%) | The neat seedling time | 1 year survival rate of seedlings (%) | The seedling outward appearance | |
| A1 | 80 | 7-10 days | 72 | Healthy and strong |
| A2 | 86.8 | 7-10 | 74 | Healthy and strong |
| A3 | 87.6 | 7-10 days | 75 | Healthy and strong |
| A31 | 88.4 | 7-10 days | 75.5 | Healthy and strong |
| A32 | 89.5 | 7-10 days | 76.2 | Healthy and strong |
| A4 | 88.6 | 7-10 days | 75.5 | Healthy and strong |
| A5 | 85.5 | 7-10 days | 73.6 | Healthy and strong |
| A6 | 84.7 | 7-10 days | 72.5 | Healthy and strong |
| B | 80.5 | 12-17 days | 70.8 | Healthy and strong |
| C | 85 | 12-17 days | 65 | Healthy and strong |
| D | 85.8 | 12-17 days | 65.8 | Healthy and strong |
| E | 86 | 12-17 days | 68 | Healthy and strong |
| F | 48 | 15-20 days | 59 | {。##.##2}, |
By above-mentioned example as can be seen, the inventive method with HORMONE TREATMENT and storage method in conjunction with after, the emergence rate height, the neat seedling time is short, seedling stalwartness, survival rate height.The inventive method emergence rate can reach more than 89%, and survival rate of seedlings can reach more than 76% in 1 year, and growth of seedling is more healthy and strong, has improved the seed availability, has saved time and cost, has improved output, far above existing technology, has fabulous application prospect.
Obviously, above detailed description of the present invention does not limit the present invention, and those skilled in the art can make various changes or distortion according to the present invention, only otherwise break away from spirit of the present invention, all belongs to the defined scope of claims of the present invention.
Claims (9)
1, a kind of method of handling fall seeding seed of Sichuan fritillary bulb is characterized in that may further comprise the steps:
A, choose then the full seed of results,, place under the lucifuge wet condition and store 3-15 week the native mixing of seeds treated;
B, taking-up seed are that the 10-80ppm Gibberellins solution is soaked with concentration;
C, the seed after will soaking dry, and can sow.
2, the method for processing fall seeding seed of Sichuan fritillary bulb according to claim 1 is characterized in that: the seed storage condition among the step a is a temperature-4 ℃～12 ℃, humidity 15%～20%.
3, the method for processing fall seeding seed of Sichuan fritillary bulb according to claim 2 is characterized in that: the seed storage condition among the step a is for to be embedded in seed in the seed bed of dark 15-60cm, Seed Layer loam cake soil layer 50cm～20cm.
4, the method for processing fall seeding seed of Sichuan fritillary bulb according to claim 3 is characterized in that: cover one deck heat and moisture preserving material on the described soil layer again.
5, the method for processing fall seeding seed of Sichuan fritillary bulb according to claim 1 is characterized in that: the usage amount of the described soil of step a is 2～6 times of volumes of seed volume, and described soil is at least a in yellow soil, detritus soil or the peat soil.
6, the method for processing fall seeding seed of Sichuan fritillary bulb according to claim 1 is characterized in that: the described Gibberellins solution concentration of step b is 10ppm～80ppm.
7, the method for processing fall seeding seed of Sichuan fritillary bulb according to claim 6 is characterized in that: the described Gibberellins solution concentration of step b is 20ppm～60ppm.
8, according to the method for claim 6 or 7 described processing fall seeding seed of Sichuan fritillary bulb, it is characterized in that: the described Gibberellins solution immersion treatment time of step b is 12 hours～72 hours.
9, the method for processing fall seeding seed of Sichuan fritillary bulb according to claim 8 is characterized in that: the described Gibberellins solution immersion treatment time of step b is 24 hours～48 hours.
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| CNB2006101721883A CN100473273C (en) | 2006-05-19 | 2006-12-31 | Method for treating autumn sowing seeds of bulbus fritillariae cirrhosae |
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| CN200610020862 | 2006-05-19 | ||
| CN200610020862.6 | 2006-05-19 | ||
| CNB2006101721883A CN100473273C (en) | 2006-05-19 | 2006-12-31 | Method for treating autumn sowing seeds of bulbus fritillariae cirrhosae |
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| CN101248730B (en) * | 2008-04-02 | 2011-01-19 | 四川新荷花中药饮片股份有限公司 | Cultivation method for completing two up-growth periodic of sichuan fritillary bulb in one year |
| CN101248746B (en) * | 2008-04-02 | 2011-01-19 | 四川新荷花中药饮片股份有限公司 | Rice seedling raising disk of sichuan fritillary bulb or sprout cultivation pocket type sexual multiplication cultivation method |
| CN101999265B (en) * | 2009-09-03 | 2012-08-08 | 薛永新 | Method for processing unibract fritillary bulb seeds |
| CN103597928A (en) * | 2013-11-21 | 2014-02-26 | 薛刚 | Dormancy after-ripening treatment method for seeds of bulbus fritillariae cirrhosae |
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| CN106489470A (en) * | 2016-09-29 | 2017-03-15 | 玉龙县金旺中药材种植有限公司 | A kind of Bulbus Fritillariae Cirrhosae method for culturing seedlings |
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| CN85108314A (en) * | 1985-11-07 | 1987-05-13 | 西北大学 | The artificial raise seedling method of fritillaria thunbergii |
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| CN101999265B (en) * | 2009-09-03 | 2012-08-08 | 薛永新 | Method for processing unibract fritillary bulb seeds |
| CN103597928A (en) * | 2013-11-21 | 2014-02-26 | 薛刚 | Dormancy after-ripening treatment method for seeds of bulbus fritillariae cirrhosae |
| CN104067790A (en) * | 2014-06-19 | 2014-10-01 | 安徽百卉园林有限公司 | Storing method for putting off seeding of stingless Chinese holly seeds |
| CN104067790B (en) * | 2014-06-19 | 2016-04-13 | 安徽百卉园林有限公司 | A kind of storage method of postponing llex cornuta lindl planting seed |
| CN105103853A (en) * | 2015-08-11 | 2015-12-02 | 玉龙县凤翔旺生态养殖有限责任公司 | Method of cultivating fritillaria cirrhosa |
| CN106489470A (en) * | 2016-09-29 | 2017-03-15 | 玉龙县金旺中药材种植有限公司 | A kind of Bulbus Fritillariae Cirrhosae method for culturing seedlings |
| CN111295968A (en) * | 2020-03-13 | 2020-06-19 | 重庆市药物种植研究所 | After-ripening method of fritillaria taipaiensis seeds |
| CN112020933A (en) * | 2020-09-18 | 2020-12-04 | 昭通市农业科学院 | Method for layering fritillaria cirrhosa seeds |
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| CN100473273C (en) | 2009-04-01 |
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