CN103597928A - Dormancy after-ripening treatment method for seeds of bulbus fritillariae cirrhosae - Google Patents

Dormancy after-ripening treatment method for seeds of bulbus fritillariae cirrhosae Download PDF

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CN103597928A
CN103597928A CN201310602145.4A CN201310602145A CN103597928A CN 103597928 A CN103597928 A CN 103597928A CN 201310602145 A CN201310602145 A CN 201310602145A CN 103597928 A CN103597928 A CN 103597928A
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dormancy
seeds
bag
fritillaria cirrhosa
seed
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CN103597928B (en
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薛刚
韩开华
余强
王强
王晓蓉
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薛刚
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Abstract

The invention provides a dormancy after-ripening treatment method for seeds of bulbus fritillariae cirrhosae. The dormancy after-ripening treatment method comprises the following steps: mixing seeds of bulbus fritillariae cirrhosae and soil for the seeds, filling into a plastic packaging bag with air pumped out, and sealing an opening of the plastic packaging bag; filling the sealed packaging bag into an air blocking protection bag with air pumped out, sealing the opening of the air blocking protection bag, and preparing the bag filled with the seeds of the bulbus fritillariae cirrhosae; placing the bag filed with the seeds of the bulbus fritillariae cirrhosae into a storage chamber, carrying out treatment away from light for 6-12 months, taking out the seeds, and soaking for 8-2 hours in water at 30-40 DEG C; filtering out the seeds, and exposing the filtered seeds at a place with illumination intensity of 4000-8000Lx for 12-24 hours, thus the seeds can be sowed. Due to the adoption of the dormancy after-ripening treatment method, the problems that in the prior art, after dormancy after-ripening treatment, the development rate of the seeds of the bulbus fritillariae cirrhosae is low, seedlings are out of order, and chemical medicine gibberellin is adopted for dormancy stopping treatment, are solved; the dormancy after-ripening treatment method is environment-friendly, no chemical medicine is needed for carrying out dormancy stopping treatment on the seeds, so that the rate of emergence of the bulbus fritillariae cirrhosae is increased, the growing condition of the bulbus fritillariae cirrhosae at the later stage is improved, and the yield of the bulbus fritillariae cirrhosae is increased.

Description

A kind of dormancy after-ripening processing method of fritillaria cirrhosa seeds
Technical field
The invention belongs to drug cultivation technical field, be specifically related to a kind of dormancy after-ripening processing method of fritillaria cirrhosa seeds.
Background technology
Bulbus Fritillariae Cirrhosae is the dry bulb of the Bulbus Fritillariae Cirrhosae of Liliaceae Fritillaria, the dark violet bulb of fritillary, the Gansu bulb of fritillary, Bulbus Fritillariae cirrhosae, taipei fritillary bulb or watt cloth bulb of fritillary.Mainly be distributed in China's Southeastern Tibet edge, western Sichuan, Zhou, macromycetes resources inLonglan area of Gansu, Deqen in Yunnan Province and some areas, Qinghai.Bulbus Fritillariae Cirrhosae is bitter, sweet, is slightly cold, and returns lung the heart channel of Hang-Shaoyin, and there is the hot phlegm in Qinghua, moisten the lung and relieve the cough, the effect of mass dissipating and swelling eliminating, for lung-heat type cough, the few phlegm of dry cough, deficiency of Yin labor is coughed, and coughs sputum streaked with blood, is a herb material of comparatively commonly using clinically.
Bulbus Fritillariae Cirrhosae is herbaceos perennial, likes the weather conditions that cool, have cold-resistant, happiness is wet, be afraid of high temperature, characteristic that happiness covers.High l5~the 50cm of plant, bulb is comprised of 2 pieces of scales, diameter l~1.5cm.Poor growth under Bulbus Fritillariae Cirrhosae wild state, in seminal propagation process, sequentially passes through seedling after seed germination, nourish and grow, reproductive growth and reaching maturity, the 1st year leaf of only growing of planting seed, elongated, be needle-like, therefore be called a pin, it within the 2nd year, is still a leaf, be long and narrow lanceolar, shape, as chicken tongue, is commonly called as chicken tongue, these 2 years is the seedling stage, and bulb growth is slow; Within the 3rd year, acrial part great majority are a leaf, are lanceolar, claim a leaf, occasionally have two leaves to claim two ribbons, and the 4th year starts to put forth, practise and claim tree son, but generally do not bloom, the 3rd year and the 4th year underground bulb growth rapid, for the bulb of fritillary nourishes and grows the more new stage; The 5th year germinate scape and flower, practise and claim Chinese enkianthus, and the solid reproductive growth that carries out.
Fritillaria cirrhosa seeds has dormancy, is difficult to breaking dormancy under wild state, make its emergence rate high, emerges neat, must must study dormancy characteristics of different and after-ripening processing method thereof.Dormancy refers to that plant corpus or its organ, in the phenomenon that certain period, metabolism and growth temporarily seized up of growing, conventionally refer in particular to by internal physiological reason and determine, even ambient temperature, the suitable phenomenon that can not sprout and grow of humidity.Bud on seed, stem (comprising bulb, stem tuber), piece root can be in resting state.Modern study has shown, after numerous seed fruit maturations, to run into rainwater or high temperature, and seed will rudiment, and this phenomenon is called tire bud.The appearance of tire bud not only affects the storage of seed, also affects growing of seed coming year, even can not sow as next year.About the after-ripening of Bulbus Fritillariae Cirrhosae, process research more, but less about its hibernation feature research.Patent 200610172188.3 discloses a kind of method of processing fall seeding seed of Sichuan fritillary bulb, the method mixes the full seed of gathering in the crops then with soil, be placed under lucifuge wet condition and store 3-15 week, then be that 10-80PPM Gibberellins solution is soaked by concentration, the seed after soaking is dried to sowing.Patent 200910164338.X discloses a kind of employing gibberellin and has hidden lamination processing method in conjunction with low temperature sand, method adopts gibberellin and hides lamination processing method in conjunction with low temperature sand, make fritillaria cirrhosa seeds energy complete physiology and modal after-ripening within a short period of time, adopt again colchicine solution to carry out multiploid induction processing to completing the fritillaria cirrhosa seeds of afterripening, cultivate the Bulbus Fritillariae Cirrhosae New MS Polyploid Variety that active constituent content is high.These two kinds of methods all have higher emergence rate and neat seedling is also comparatively neat, but all used gibberellin in these two kinds of methods, it is on the dormancy mechanism of fritillaria cirrhosa seeds that gibberellin belongs to chemicals for what impact, whether there are the problems such as lamination effect not all to be confirmed, and then are not used widely in industrialized production.Therefore, in industrialized production, need a kind ofly new can improve percentage of seedgermination, the regularity of emerging, and drug residue free, the fritillaria cirrhosa seeds processing method of environmental protection.
Summary of the invention
For solve prior art fritillaria cirrhosa seeds dormancy after-ripening process after seed development rate low, the irregular and prior art of Qi Miao adopts chemicals gibberellin break to sleep the problem of processing.The invention provides a kind of without adopting chemicals or adopting conventional natural lamination method to carry out the broken method of sleeping and processing of after-ripening to seed, the method environmental protection, can improve the emergence rate of Bulbus Fritillariae Cirrhosae, improve the upgrowth situation in Bulbus Fritillariae Cirrhosae later stage, improve the output of Bulbus Fritillariae Cirrhosae.
A dormancy after-ripening processing method for fritillaria cirrhosa seeds, it comprises the following steps:
S1: fritillaria cirrhosa seeds is mixed with soil with seed, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 6~12 months;
S4: seed after S3 step process is taken out, and is to soak 8~12h in the water of 30~40 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 4000~8000Lx irradiates 12~24h, can sow.
Preferably, the seed described in S1 step is 2~3 times of fritillaria cirrhosa seeds volume by native usage amount, described plantation by native humidity lower than 20%.
Preferably, the seed described in S1 step is at least one of humus soil, loess or sandy soil with soil.
Preferably, in S2 step, in pumping choke protective bag, after air, in bag, be filled with carbon dioxide, and then sealing sack is prepared into fritillaria cirrhosa seeds bag;
Preferably, the lucifuge processing time described in S3 step is 8~10 months;
Preferably, the lucifuge described in S3 step is processed indoor temperature and is remained on 6~15 ℃, and humidity remains on 20~40%.
Preferably, the temperature of the water described in S4 step is 30~35 ℃, soak time 8~10h.
Preferably, the intensity of illumination described in S5 step is 4000~6000Lx, and described irradiation time is 12~16h.
Bulbus Fritillariae Cirrhosae has hibernation feature, and seed its germination rate when not carrying out after-ripening processing is very low.Prove by experiment seed is placed under vacuum condition and is stored, reduce seed and the external world and carry out gas exchange, reduce seed self metabolic activity, make seed in metastable dormancy environment.After packaging bag is vacuumized, then packaging bag is packed in choke protective bag, pump air in choke protective bag, then in choke protective bag, be full of carbonic acid gas, hermetically storing.Prove by experiment, Bulbus Fritillariae Cirrhosae is meeting release of carbon dioxide in the process of metabolism, in seed storage outer bag, be filled with carbon dioxide, while making outer bag gas concentration lwevel store the concentration of inner bag higher than seed, the biologically active that can not only keep Bulbus Fritillariae Cirrhosae, also be conducive to fritillaria cirrhosa seeds epidermis and the release of inner growth mortifier, and then improve the germination rate of seed simultaneously.
It is that the water of 30~40 ℃ soaks 8~12h that seed is put into temperature, main purpose is further to discharge fritillaria cirrhosa seeds epidermis and inner germination inhicbitor by emerge in worm water, further fritillaria cirrhosa seeds is broken to sleep and process, the water temperature that shows by experiment 30~35 ℃ is processed 8~10h, and its immersion effect is the most remarkable.
It is that 4000~8000Lx irradiates 12~24h that seed is placed on to intensity of illumination, and main purpose is further to play brokenly dormancy effect by illumination.
The invention has the beneficial effects as follows: processing method of the present invention is not used chemicals or other organic solvent, nontoxic to soil, also do not have the problem of Bulbus Fritillariae Cirrhosae medicament residue.The present invention adopts vacuum condition to add carbon dioxide sequestration method storage seed, keeping the bioactive while of Bulbus Fritillariae Cirrhosae, can promote fritillaria cirrhosa seeds epidermis and inner germination inhicbitor to discharge, make fully broken dormancy of seed, improve germination rate and the neat seedling regularity of seed.The inventive method step is simple, with low cost, is convenient to industrialization promotion on a large scale.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, and protection scope of the present invention is not limited to the following stated:
Embodiment 1: the dormancy after-ripening processing method of fritillaria cirrhosa seeds of the present invention, and it comprises the following steps:
S1: fritillaria cirrhosa seeds is mixed with soil with seed, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 6~12 months;
S4: seed after S3 step process is taken out, and is to soak 8~12h in the water of 30~40 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 4000~8000Lx irradiates 12~24h, can sow.
Wherein the temperature of ,S3 step locker room remains on 6~15 ℃, and humidity remains on 20~40%.
Embodiment 2: the dormancy after-ripening processing method of fritillaria cirrhosa seeds of the present invention, and it comprises the following steps:
S1: fritillaria cirrhosa seeds is mixed with soil with seed, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, be filled with carbon dioxide in bag, and then sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 6~12 months, lucifuge is processed indoor temperature and remained on 6~15 ℃, and humidity remains on 20~40%;
S4: seed after S3 step process is taken out, and is to soak 8~12h in the water of 30~40 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 4000~8000Lx irradiates 12~24h, can sow.
Embodiment 3: the dormancy after-ripening processing method of fritillaria cirrhosa seeds of the present invention, and it comprises the following steps:
S1: 2~3 times of humidity of fritillaria cirrhosa seeds and fritillaria cirrhosa seeds volume are mixed lower than 20% humus soil, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, be filled with carbon dioxide in bag, and then sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 6~12 months, lucifuge is processed indoor temperature and remained on 6~15 ℃, and humidity remains on 20~40%;
S4: seed after S3 step process is taken out, and is to soak 8~12h in the water of 30~40 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 4000~8000Lx irradiates 12~24h, can sow.
Embodiment 4: the dormancy after-ripening processing method of fritillaria cirrhosa seeds of the present invention, and it comprises the following steps:
S1: 2 times of humidity of fritillaria cirrhosa seeds and fritillaria cirrhosa seeds volume are mixed lower than 20% sandy soil, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, be filled with carbon dioxide in bag, and then sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 6 months, lucifuge is processed indoor temperature and remained on 6 ℃, and humidity remains on 20~25%;
S4: seed after S3 step process is taken out, and is to soak 8~12h in the water of 30~35 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 4000~6000Lx irradiates 12h, can sow.
Embodiment 5: the dormancy after-ripening processing method of fritillaria cirrhosa seeds of the present invention, and it comprises the following steps:
S1: 2 times of humidity of fritillaria cirrhosa seeds and fritillaria cirrhosa seeds volume are mixed lower than 20% humus soil, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, be filled with carbon dioxide in bag, and then sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 8 months, lucifuge is processed indoor temperature and remained on 8 ℃, and humidity remains on 20~30%;
S4: seed after S3 step process is taken out, and is to soak 10h in the water of 30~35 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 4000~8000Lx irradiates 16h, can sow.
Embodiment 6: the dormancy after-ripening processing method of fritillaria cirrhosa seeds of the present invention, and it comprises the following steps:
S1: 3 times of humidity of fritillaria cirrhosa seeds and fritillaria cirrhosa seeds volume are mixed lower than 20% loess, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, be filled with carbon dioxide in bag, and then sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 10 months, lucifuge is processed indoor temperature and remained on 10 ℃, and humidity remains on 30~40%;
S4: seed after S3 step process is taken out, and is to soak 12h in the water of 30~40 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 4000~6000Lx irradiates 20h, can sow.
Embodiment 7: the dormancy after-ripening processing method of fritillaria cirrhosa seeds of the present invention, and it comprises the following steps:
S1: 2 times of humidity of fritillaria cirrhosa seeds and fritillaria cirrhosa seeds volume are mixed lower than 20% humus soil, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, be filled with carbon dioxide in bag, and then sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 12 months, lucifuge is processed indoor temperature and remained on 12 ℃, and humidity remains on 30~40%;
S4: seed after S3 step process is taken out, and is to soak 10h in the water of 35~40 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 6000~8000Lx irradiates 24h, can sow.
Embodiment 8: the dormancy after-ripening processing method of fritillaria cirrhosa seeds of the present invention, and it comprises the following steps:
S1: 3 times of humidity of fritillaria cirrhosa seeds and fritillaria cirrhosa seeds volume are mixed lower than 20% humus soil, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, be filled with carbon dioxide in bag, and then sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 8 months, lucifuge is processed indoor temperature and remained on 15 ℃, and humidity remains on 20~30%;
S4: seed after S3 step process is taken out, and is to soak 8h in the water of 30~40 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 6000~8000Lx irradiates 24h, can sow.
Embodiment 9: the dormancy after-ripening processing method of fritillaria cirrhosa seeds of the present invention, and it comprises the following steps:
S1: 2~3 times of humidity of fritillaria cirrhosa seeds and fritillaria cirrhosa seeds volume are mixed lower than 20% humus soil, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, be filled with carbon dioxide in bag, and then sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 10 months, lucifuge is processed indoor temperature and remained on 8 ℃, and humidity remains on 20~30%;
S4: seed after S3 step process is taken out, and is to soak 12h in the water of 30~40 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 6000~8000Lx irradiates 12h, can sow.
Embodiment 10: the dormancy after-ripening processing method of fritillaria cirrhosa seeds of the present invention, and it comprises the following steps:
S1: 3 times of humidity of fritillaria cirrhosa seeds and fritillaria cirrhosa seeds volume are mixed lower than 20% humus soil, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, be filled with carbon dioxide in bag, and then sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 6~12 months, lucifuge is processed indoor temperature and remained on 6~15 ℃, and humidity remains on 30~40%;
S4: seed after S3 step process is taken out, and is to soak 8~12h in the water of 30~40 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 6000~8000Lx irradiates 24h, can sow.
Below by experiment, further illustrate effect of the present invention:
One, Bulbus Fritillariae Cirrhosae germination inhicbitor experiment
1, experiment overview
The medicinal material wild base of fostering in En Weigao source, Kangding is positioned at Dardo County Xin Douqiao town, Tibetan Autonomous Prefecture of Garze, and the about 3500m of height above sea level belongs to Hilly Plateau area, is the continental monsoon climate of plateau type, 6.2 ℃ of average annual temperature.Seed picks up from this wild proving ground of fostering, and gathers tendril-leaved fritillary bulb fruit, kept dry annual mid-August to the last ten-days period.
2, experiment grouping and method
Whole experiment is divided into 5 processed group, lixiviate A group, lixiviate B group, lixiviate C group, lixiviate D group, distilled water group, front 4 processed group are 1.0g/20ml by treatment concentration, and the 5th processed group is by concentrated 4 times of leaching liquor, and extracting method is for to be placed in 60 ℃ of water-bath lixiviate 24h by fritillaria cirrhosa seeds.Observe and respectively organize the inhibitory action of Bulbus Fritillariae Cirrhosae leaching liquor to Chinese cabbage seed.Broadcast Chinese cabbage seed 5O grain, be placed in 25 ℃ of constant incubators and cultivate, after 24h, observe the situation that Chinese cabbage seed germinates, after 48h, measure radicle length, computational short cut vitality index [vitality index=final radicel length (cm) * germination rate (%) germinates in simplification], every extract germination test repeats for 3 times.In fritillaria cirrhosa seeds, the leach extraction method of germination inhicbitor is in Table 1.
The leach extraction method of germination inhicbitor in table 1 fritillaria cirrhosa seeds
3, experimental result
Contrast and compare with distilled water, the water extract of fritillaria cirrhosa seeds and alcohol leaching liquor dialogue dish seed sprouting all have inhibitory action, and the growth of seed base-root (simplification vitality index) is had to extremely significantly inhibitory action.Pulverize fritillaria cirrhosa seeds water extract the inhibitory action (extraction B group) of Chinese cabbage seed germination is significantly better than to complete fritillaria cirrhosa seeds leaching liquor (extraction A, D), show that fritillaria cirrhosa seeds germination inhicbitor does not exist only in seed epidermis, the Interior Seeds that are present in [referring to Li Rong more, Ye Yong. seed dormancy and broken dormancy Progress in Mechanism [J]. northwest Botany Gazette, 2005,25 (1): 2350-2355.].Under the pulverizing fritillaria cirrhosa seeds leaching liquor of 80% methyl alcohol, Chinese cabbage seed germination rate, only lower than distilled water contrast 5.7%, does not reach significance level, so fritillaria cirrhosa seeds germination inhicbitor is mainly water soluble compound but not alcohol soluble compound.The results are shown in Table 2.
The impact that the different fritillaria cirrhosa seeds leaching liquors of table 2 are sprouted Chinese cabbage seed
Experiment group Fresh not Germinating Seeds (%) Germination rate (%) Simplify vitality index (cm%)
Distilled water contrast 0.0 99.0a* 108.5A
Lixiviate A group 1.3 94.7ab 77.3B
Lixiviate B group 3.3 52.0c 25.7C
Lixiviate C group 1.7 93.3ab 76.4D
Lixiviate D group 0.7 92.7b 60.4F
Lixiviate E group 1.7 12.0d 4.3G
* adopt correction least significant difference method (LSD) to carry out test of significance, the different lowercase alphabets of same column show the significance of difference, P >=5%, and the different capitalizations of same column represent the significance of difference, P >=1%.
Two, the control experiment of different after-ripening processing methods
1, experiment overview
The medicinal material wild base of fostering in En Weigao source, Kangding is positioned at Dardo County Xin Douqiao town, Tibetan Autonomous Prefecture of Garze, and the about 3500m of height above sea level belongs to Hilly Plateau area, is the continental monsoon climate of plateau type, 6.2 ℃ of average annual temperature.
2, experiment grouping
Whole test is divided into 10 experimental group, seed prepared by dormancy after-ripening processing method of the present invention is divided into experiment A group, experiment B group, experiment C group, experiment D group, experiment E group, experiment F group, the standby seed of lamination legal system is divided into experiment G group, experiment H group, experiment I group, sets up in addition J group for blank group.
3, experimental technique
The present invention tests A group dormancy after-ripening processing method: S1: 2 times of humidity of fritillaria cirrhosa seeds and fritillaria cirrhosa seeds volume are mixed lower than 20% humus soil, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack; S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, be filled with carbon dioxide in bag, and then sealing sack is prepared into fritillaria cirrhosa seeds bag; S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 1 month, lucifuge is processed indoor temperature and remained on 8 ℃, and humidity remains on 20~30%; S4: seed after S3 step process is taken out, and is to soak 10h in the water of 30~35 ℃ by temperature; S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 4000~8000Lx irradiates 16h.Seed after processing, with pulverizing, is added to 8 times of amount distilled water lixiviate 12h, leaching liquor is concentrated and is formulated as the leaching liquor that concentration is 0.1g/ml.
Experiment B group dormancy after-ripening processing method is: except S3 step, put into locker room and carry out lucifuge processing in 3 months, all the other treatment steps and condition are all with experiment A group.
Experiment C group dormancy after-ripening processing method is: except S3 step, put into locker room and carry out lucifuge processing in 5 months, all the other treatment steps and condition are all with experiment A group.
Experiment D group dormancy after-ripening processing method is: except S3 step, put into locker room and carry out lucifuge processing in 6 months, all the other treatment steps and condition are all with experiment A group.
Experiment E group dormancy after-ripening processing method is: except S3 step, put into locker room and carry out lucifuge processing in 7 months, all the other treatment steps and condition are all with experiment A group.
Experiment F group dormancy after-ripening processing method is: except S3 step, put into locker room and carry out lucifuge processing in 8 months, all the other treatment steps and condition are all with experiment A group.
Experiment G group: take from 3 months seeds of right lamination, wash, be dried and process rear directly pulverizing, add 8 times of amount distilled water lixiviate 12h, leaching liquor is concentrated and is formulated as the leaching liquor that concentration is 0.1g/ml.
Experiment H group: take from 5 months seeds of right lamination, wash, be dried and process rear directly pulverizing, add 8 times of amount distilled water lixiviate 12h, leaching liquor is concentrated and is formulated as the leaching liquor that concentration is 0.1g/ml.
Experiment I group: take from 8 months seeds of right lamination, wash, be dried and process rear directly pulverizing, add 8 times of amount distilled water lixiviate 12h, leaching liquor is concentrated and is formulated as the leaching liquor that concentration is 0.1g/ml.
After above-mentioned each group experiment leaching liquor is ready to, observes and respectively organize the inhibitory action of leaching liquor to Chinese cabbage seed.Experiment J group: splash in germination box with distilled water.Every group is divided into and joins 20 Chinese cabbage seeds, packs in germination box every group of 1 germination box into.Be placed in 25 ℃ of constant incubators and cultivate, after 24h, observe Chinese cabbage seed germination, calculate germination rate.
4, experimental result
Experimental result shows that vacuum of the present invention adds carbon dioxide sequestration method and processes after seed, prolongation along with shelf time, germination inhicbitor in Bulbus Fritillariae Cirrhosae epidermis and fruit discharges and increases gradually, and after 6 months storages are processed, the germination inhicbitor in Bulbus Fritillariae Cirrhosae epidermis and fruit is tending towards constant.Control group also can discharge the germination inhicbitor in Bulbus Fritillariae Cirrhosae epidermis and fruit after lamination is processed.In same treatment, in the time, the germination inhicbitor that the inventive method discharges is apparently higher than the fritillaria cirrhosa seeds of lamination method processing, and then the fritillaria cirrhosa seeds that explanation the present invention processes breaks the broken dormancy effect that dormancy successful is better than traditional lamination processing method.The results are shown in Table 3.
The impact that each experimental group seed leaching liquor of table 3 is sprouted Chinese cabbage seed
Group Sample number (grain) Germination seed (grain) not Chinese cabbage seed germination rate (%)
Experiment A group 50 42 16
Experiment B group 50 24 52
Experiment C group 50 10 80
Experiment D group 50 5 90
Experiment E group 50 4 92
Experiment F group 50 4 92
Experiment G group 50 30 40
Experiment H group 50 23 54
Experiment I group 50 9 82
Experiment J group 50 1 98
As can be seen from Table 3, each leaching liquor group of Bulbus Fritillariae Cirrhosae is germinateed and is all had inhibitory action in various degree Chinese cabbage seed.When Bulbus Fritillariae Cirrhosae is processed 1 month according to the inventive method, the germination rate of Chinese cabbage seed is only 16%; While processing 3 months, the germination rate of Chinese cabbage seed is only 52%; While processing 5 months, the germination rate of Chinese cabbage seed is 80%; While processing 6 months, the germination rate of Chinese cabbage seed is only 90%; While processing 7 months, the germination rate of Chinese cabbage seed is 92%; While processing 8 months, the germination rate of Chinese cabbage seed is 92%.Show that according to vacuum of the present invention, adding carbon dioxide sequestration method processes after seed, prolongation along with shelf time, germination inhicbitor in Bulbus Fritillariae Cirrhosae epidermis and fruit discharges more, and after 6 months storages are processed, the germination inhicbitor in Bulbus Fritillariae Cirrhosae epidermis and fruit is tending towards constant.When control group is processed 3 months in lamination, the germination rate of Chinese cabbage seed is only 40%; While processing 5 months, the germination rate of Chinese cabbage seed is 54%; While processing 8 months, the germination rate of Chinese cabbage seed is 82%; Show that the processing of lamination method also can discharge the germination inhicbitor in Bulbus Fritillariae Cirrhosae epidermis and fruit.
Four, the comparative study of processing method of the present invention and gibberellin processing method
1, experiment grouping
Whole test is divided into 6 experimental group, and seed prepared by dormancy after-ripening processing method of the present invention is divided into experiment A group, experiment B group, experiment C group, D group, and seed prepared by gibberellin is divided into experiment E group, experiment F group, experiment G group.
2, experimental technique
The present invention tests A group dormancy after-ripening processing method: S1: 2 times of humidity of fritillaria cirrhosa seeds and fritillaria cirrhosa seeds volume are mixed lower than 20% humus soil, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack; S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, be filled with carbon dioxide in bag, and then sealing sack is prepared into fritillaria cirrhosa seeds bag; S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 5 months, lucifuge storage indoor temperature remains on 8 ℃, and humidity remains on 20~30%; S4: seed after S3 step process is taken out, and is to soak 10h in the water of 30~35 ℃ by temperature; S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 4000~8000Lx irradiates 16h.Getting 120 seeds tests.
Experiment B group dormancy after-ripening processing method is: except S3 step, put into locker room and carry out lucifuge processing in 6 months, all the other treatment steps and condition are all with experiment A group.Getting 120 seeds tests.
Experiment C group dormancy after-ripening processing method is: except S3 step, put into locker room and carry out lucifuge processing in 7 months, all the other treatment steps and condition are all with experiment A group.Getting 120 seeds tests.
Experiment D group dormancy after-ripening processing method is: except S3 step, put into locker room and carry out lucifuge processing in 8 months, all the other treatment steps and condition are all with experiment A group.Getting 120 seeds tests.
Experiment E group processing method: seed is done dipping pretreatment with gibberellin to fritillaria cirrhosa seeds after adopting conventional after-ripening to process, and processes 32h with low concentration 20ug/ml.Concrete steps are participated in: Song Tingjie, Xiao Jieyi, Li Xiangzhou. and fritillaria cirrhosa seeds gibberellin is processed experimental study [J]. the Chinese medicine magazine .1994 of basic unit, 8 (I): 14~15. get 120 seeds tests.
Experiment F group processing method: except gibberellin concentration is that 30ug/ml processes 32h, all the other condition steps are all with experiment E group.Getting 120 seeds tests.
Experiment G group processing method: except gibberellin concentration is that 40ug/ml processes 32h, all the other condition steps are all with experiment E group.Getting 120 seeds tests.
According to the whole ground of the method (referring to Bulbus Fritillariae Cirrhosae production technology regulation DB51/T900-2009) of conventional Bulbus Fritillariae Cirrhosae plantation, open railway carriage or compartment, ditching, cultivation, according to identical method, plant, after plantation according to same daily management mode.
3, detect index
In experimentation, record the indexs such as whether germination rate (%), neat seedling time, seedling healthy and strong.Experimental result is in Table 4.
4, experimental result
The comparative study of each experimental group germination rate of table 4, neat seedling time
Experimental result shows, by the inventive method, process the neat seedling time shorten of seed after fritillaria cirrhosa seeds, germination rate obviously increases, show that the broken dormancy effect of processing fritillaria cirrhosa seeds with broken dormancy effect and the gibberellin of the inventive method processing fritillaria cirrhosa seeds is comparatively approaching, in actual production, can substitute the application of gibberellin, to reach, reduce the pollution of soil and stop the problem of medicinal material residue of pesticide.

Claims (8)

1. a dormancy after-ripening processing method for fritillaria cirrhosa seeds, is characterized in that: it comprises the following steps:
S1: fritillaria cirrhosa seeds is mixed with soil with seed, pack in plastic packaging bag, then pump the air of packaging bag inside, sealing sack;
S2: S1 step has been sealed to complete packaging bag and reinstalled in choke protective bag, pumped air in choke protective bag, sealing sack is prepared into fritillaria cirrhosa seeds bag;
S3: fritillaria cirrhosa seeds bag is put into locker room and carry out lucifuge processing in 6~12 months;
S4: seed after S3 step process is taken out, and is to soak 8~12h in the water of 30~40 ℃ by temperature;
S5: the seed after 4 step process is leached, and being placed on intensity of illumination is that 4000~8000Lx irradiates 12~24h, can sow.
2. the dormancy after-ripening processing method of a kind of fritillaria cirrhosa seeds according to claim 1, is characterized in that: the seed described in S1 step is 2~3 times of fritillaria cirrhosa seeds volume by native usage amount, described plantation by native humidity lower than 20%.
3. the dormancy after-ripening processing method of a kind of fritillaria cirrhosa seeds according to claim 1, is characterized in that: the seed described in S1 step is at least one of humus soil, loess or sandy soil with soil.
4. the dormancy after-ripening processing method of a kind of fritillaria cirrhosa seeds according to claim 1; it is characterized in that: in S2 step; in pumping choke protective bag, after air, in bag, be filled with carbon dioxide, and then sealing sack is prepared into fritillaria cirrhosa seeds bag.
5. the dormancy after-ripening processing method of a kind of fritillaria cirrhosa seeds according to claim 1, is characterized in that: the lucifuge processing time described in S3 step is 8~10 months.
6. the dormancy after-ripening processing method of a kind of fritillaria cirrhosa seeds according to claim 1, is characterized in that: the lucifuge described in S3 step is processed indoor temperature and remained on 6~15 ℃, and humidity remains on 20~40%.
7. the dormancy after-ripening processing method of a kind of fritillaria cirrhosa seeds according to claim 1, is characterized in that: the temperature of the water described in S4 step is 30~35 ℃, soak time 8~10h.
8. the dormancy after-ripening processing method of a kind of fritillaria cirrhosa seeds according to claim 1, is characterized in that: the intensity of illumination described in S5 step is 4000~6000Lx, and described irradiation time is 12~16h.
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CN104285748A (en) * 2014-09-10 2015-01-21 吴晖 Method for improving survival rate of pachira macrocarpa seed seedling
CN105103853A (en) * 2015-08-11 2015-12-02 玉龙县凤翔旺生态养殖有限责任公司 Method of cultivating fritillaria cirrhosa
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CN104285748A (en) * 2014-09-10 2015-01-21 吴晖 Method for improving survival rate of pachira macrocarpa seed seedling
CN105103853A (en) * 2015-08-11 2015-12-02 玉龙县凤翔旺生态养殖有限责任公司 Method of cultivating fritillaria cirrhosa
CN105325151A (en) * 2015-11-04 2016-02-17 巫溪县瑞雪药材种植有限责任公司 Tendril-leaved fritillary bulb planting method
CN105453753A (en) * 2015-11-26 2016-04-06 丽江云鑫绿色生物开发有限公司 Processing method of fritillaria praewalskii maxim ex batal seeds and fritillaria praewalskii maxim ex batal culture method
CN105453753B (en) * 2015-11-26 2021-04-13 丽江云鑫绿色生物开发有限公司 Method for treating fritillaria cirrhosa seeds and fritillaria cirrhosa cultivation method
CN111295968A (en) * 2020-03-13 2020-06-19 重庆市药物种植研究所 After-ripening method of fritillaria taipaiensis seeds
CN112243631A (en) * 2020-09-14 2021-01-22 云南省农业科学院花卉研究所 Method for rapidly breaking dormancy of green flower lily seed bulbs
CN112020933A (en) * 2020-09-18 2020-12-04 昭通市农业科学院 Method for layering fritillaria cirrhosa seeds

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