CN105230181A - Technology utilizing hydrogen peroxide to improve germination rate of cinnamomum camphora - Google Patents
Technology utilizing hydrogen peroxide to improve germination rate of cinnamomum camphora Download PDFInfo
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- CN105230181A CN105230181A CN201510635768.0A CN201510635768A CN105230181A CN 105230181 A CN105230181 A CN 105230181A CN 201510635768 A CN201510635768 A CN 201510635768A CN 105230181 A CN105230181 A CN 105230181A
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- seed
- seeds
- germination rate
- hydrogen peroxide
- camphor tree
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Abstract
The invention discloses a technology utilizing hydrogen peroxide to improve the germination rate of cinnamomum camphora. The technology mainly comprises the following steps: (1) harvesting seeds and processing seeds; (2) performing seed stratification; (3) accelerating seed germination; (4) sowing the seeds on a seed bed; (5) carrying out seedling stage management; (6) transplanting seedlings and performing field planting; (7) managing and nursing nursery stocks. Through a hydrogen peroxide treatment, the seed permeability is improved, and thus water and oxygen gas can enter the seed embryo more easily. The seeds, which are processed by H2O2 (5%) for 4 to 6 hours, can germinate 7 to 10 days before the unprocessed seeds, the germination potential is increased by 13.2%, and the germination rate is increased by 20.1%.
Description
Technical field
The present invention relates to plant seed germination technical field, particularly one utilizes hydrogen peroxide to improve fragrant camphor tree seed germination rate technology.
Technical background
Fragrant camphor tree is aiphyllium, and tree crown is extensively opened up, and is thick with leaves, spectacular, is excellent street tree and shade tree, have that very strong smoking lays the dust, water conservation, solid native sand prevention and the ability of beautifying the environment.Camphor tree happiness light, slightly resistance to shade; Happiness warm and moist weather, cold resistance is not strong, requires not tight to soil, more water-fast wet, but not drought-resistant, barren and saline-alkali soil.The florescence 4-5 month, the fruit phase 10-11 month; Spherical fruitlet maturation is black purple afterwards, diameter about 0.5 centimetre.Fragrant camphor tree seed is because of containing sprouting inhibitor material with plant the hard and germination rate reduction of shell, and rudiment is irregular.Especially some northern territory spring temperatures are on the low side, and soil moisture content is low, inclined saline alkali, poor nutritional, are unfavorable for fragrant camphor tree large area seed propagation.
There is pertinent literature to disclose some at present and improve fragrant camphor tree seed germination rate and the technology of doing sth. in advance rudiment, but applicant carries out actual verification repeatedly through published technology, fragrant camphor tree seed can not obtain the germination rate of describe in as public technology more than 80% at China's northern territory germination rate, germination rate can only be made by prior art to reach 55%-65% to presprouting of seeds process.
Summary of the invention
The present invention is directed to the problem in background technology, have studied one and utilize hydrogen peroxide to improve fragrant camphor tree seed to sprout
Bud rate technology, effectively removes the rudiment inhibiting substances of Interior Seed by cool stratification, fragrant camphor tree seed germination rate can be made to reach 80%-85%.
The technology of the present invention solution:
One utilizes hydrogen peroxide to improve fragrant camphor tree seed germination rate technology, mainly comprise the following steps: (1), seed collecting and the production of hybrid seeds, (2), earthing of seeds process, (3), presprouting of seeds process, (4), seeding in nursery bed, (5), seedling management, (6), to transplant seedlings and field planting, (7), nursery stock management and maintenance, it is characterized in that: described presprouting of seeds process comprises the following steps: first by the emerge in worm water 2h of seed with 50 DEG C during vernalization, make the moisture of neutron absorption abundance, then put into container, by the H of the standard 1%-5% of 1000ml-1200ml/kg
2o
2process, temperature is 25-28 DEG C, and humidity is 55-65%, cleans afterwards, for sowing with clear water.
Further, described seeding in nursery bed comprises the following steps: sanding gravel and fine sand under nursery pond, upper strata paving 15cm thickness seedling medium, and seedling medium presses fertilizer: the standard of vermiculite=3:1, the wide 1.2-1.5m in general seedbed, and length is how many depending on seed; Seed drilling is watered permeable the previous day, and first on furrow face, first open a ditch during sowing, minor groove width is generally 10-12cm, the degree of depth is 4-5cm, and bar ditch spacing 15-20cm, carries out seed program request, plant spacing and be generally 4-5cm, often row broadcasts 30-40 grain, after every bar ditch seed finishes, and earthing leveling also Shi Dang Hong water, with lose seed not ponding be advisable, use with good conditionsi ground temperature heating wire, maintenances substrate temperature is 30-35 DEG C, the initial stage for being incubated, moisturizing can take Small plastic shed or lid straw screen or mat.General 15-20 days can start rudiment, and a large amount of rudiment in 28-32 days, when 60 days, germination rate reaches more than 80%.
Existing type of seeding general 22-30 days starts rudiment, a large amount of rudiment in 40-45 days, and when 60 days, germination rate is 55-65%.Adopt that seedbed+fertilizer+controllably temperature heating wire+automatic sprinkling system pattern carries out seeding and seedling raising can provide enough temperature and moisture for seed germination, enough nutritional conditions can be provided for the seedling after rudiment.
As preferably, described H
2o
2concentration is 5%, and the processing time is 4-6h.The concentration of 5% is optimal selection, and Some seeds then can be made to lose vigor higher than 5% concentration, effect is lower than not using H
2o
2time effect; And lower than the H of 5% concentration
2o
2processing time is then longer than 4-6h, but effect is all better than and does not use H
2o
2time situation.
Beneficial effect of the present invention:
This technology increases seed permeability by hydrogen peroxide treatment, promotes that moisture and oxygen enter embryo, utilizes 5%H
2o
2process 4-6h shifts to an earlier date rudiment 7-10 days than traditional seedling-cultivating method, improves rudiment gesture 13.2%, improves germination rate 20.1%.
Embodiment
Specifically the present invention is set forth below in conjunction with embodiment.
The present embodiment is on the low side for northern territory spring temperature, and soil moisture content is low, inclined saline alkali, and this technology that the factors such as poor nutritional draw through repetition test specifically comprises the following steps:
(1), seed collecting and the production of hybrid seeds
Fragrant camphor tree is collected seed and should choose the normal adult healthy tree of growth, the annual the first tenday period of a month in November, when fruit maturation becomes atropurpureus, gather in time, wash by rubbing with the hands with after the emerge in worm water 2d of 35-40 DEG C, remove pericarp, exosper and natant seed are removed in drift, after being rinsed well by the full seed water sunk under water, mix ash degreasing 12h, clean shady dry.
(2), earthing of seeds process
Seed is rinsed well with water after first soaking 2h with 0.5% liquor potassic permanganate again, by clear water sand (wet husky): seed is that the standard of 3:1 (water content about 30%) is mixed thoroughly, is contained in Polywoven Bag.Select leeward area without shade in outdoor, dig the storage of dark 100-120cm heatable adobe sleeping platform, stay the careless handle of ventilation, carry out mark, writing time.Check once depending on the every 10-15d of weather condition, and suitably water, prevent from going mouldy, dehydration and the plague of rats.
(3), presprouting of seeds process
Wet seed of hiding takes out after the 90-100d time preserves, and after screening out fine sand, is chosen by seed, remove the seed that goes rotten, carry out vernalization process with sieve.First by the emerge in worm water 2h of seed with 50 DEG C during vernalization, make the moisture of neutron absorption abundance.Put into container (how much select glass container or Plastic Drum depending on seed, container can be placed in illumination box) again, by 1000ml-1200ml/kg standard with 5% H
2o
2process 4-6h, temperature is 25-28 DEG C, and humidity is 55-65%, cleans afterwards, for sowing with clear water.
(4), seeding in nursery bed
Sanding gravel and fine sand under nursery pond, upper strata paving 15cm thickness seedling medium, seedling medium presses fertilizer: the standard of vermiculite=3:1, the wide 1.2-1.5m in general seedbed, and length is how many depending on seed.Seed drilling is watered permeable the previous day, and first on furrow face, first open a ditch during sowing, minor groove width is generally 10-12cm, the degree of depth is 4-5cm, and bar ditch spacing 15-20cm, carries out seed program request, plant spacing and be generally 4-5cm, often row broadcasts 30-40 grain, after every bar ditch seed finishes, and earthing leveling also Shi Dang Hong water, with lose seed not ponding be advisable, use with good conditionsi ground temperature heating wire, maintenances substrate temperature is 30-35 DEG C, the initial stage for being incubated, moisturizing can take Small plastic shed or lid straw screen or mat.General 15-20 days can start rudiment, and a large amount of rudiment in 28-32 days, germination rate reaches more than 80%.
(5), seedling management
After having a small amount of kind bud to be unearthed, when cloudy day or dusk, remove straw screen or mat or canopy film.Strengthen weeding in the process that comes up, loosen the soil and water, be strictly on guard against ponding.Should note during uprooting weed not taken out of by seedling, the initial stage of loosening the soil should be shallow, and the later stage is slightly dark, is advisable not hinder root.For the anti-damping-off of seedling and broken disease, spray 1 time, continuous 3-4 time with carbendazim 800-1000 times liquid weekly.Period successively can use 0.5% urea and each 1 time of 0.5% biphosphate potassium foliage fertilizer.
(6), to transplant seedlings and field planting
Fragrant camphor tree main root is flourishing, and side root is few, when seedling grows to 10-15cm(4-5 sheet true leaf) time, main root must be cut off, carry out first time and transplant seedlings.North Jiangsu Area soil is comparatively barren, inclined saline alkali, thus should use compost 8-10 ton/mu (being advisable with the chicken manure become thoroughly decomposed) before fragrant camphor tree is transplanted, then loosen the soil smooth, field planting, because fragrant camphor tree branching-point is lower, be applicable to dense planting, seeding row spacing is advisable with the standard of 20cm × 25cm.Transplanting time should be selected in afternoon without strong wind time, with good conditionsily build sunshade net, or carry out automatic irrigation.
(7), nursery stock management and maintenance
Easily there is iron chlorosis phenomenon in fragrant camphor tree seedling, thus after field planting one week, (now completely slow seedling) can execute a small amount of thin liquid dung, can spray 1% chelated iron 2 times/month from second week, regularly weed with a hoe, water in good time, and wipe out low level bud or strong prosperous side shoot in time, to promote trunk grow.Ditch spread in October base manure, late November to early December scaffolding antifreeze, upper cover film or add a cover straw screen or mat, also can build duplicature.The height of canopy is advisable not weigh seedling wounded, simultaneously mulching straw between seedling, with safe overwintering.Can to reach 50-60cm high for raw seedling then.
In March, the 3rd seedling is carried out third time to transplant, seeding row spacing is advisable with 50cm × 60cm, will be with sufficient soil during transplanting, goes to force to stay on weak during pruning, ensures end advantage, its rapid shaping short.Management will note insect protected diseases prevention, fertilising, excision lateral bud.
Experimental data:
Medicament: H
2o
2
Table 1: variable concentrations H
2o
2process 4h is on the impact of fragrant camphor tree seed germination rate and rudiment gesture
Note: CK is not for use H
2o
2seed sprouting situation during process
Table 2 different disposal H
2o
2time is on the impact of fragrant camphor tree rudiment gesture
Table 3 different disposal H
2o
2time is on the impact of fragrant camphor tree germination rate
This research removes the rudiment inhibiting substances of Interior Seed by cool stratification, and innovation is to increase seed permeability by hydrogen peroxide treatment, promotes that moisture and oxygen enter embryo, utilizes 5%H
2o
2process 4-6h shifts to an earlier date rudiment 7-10 days than traditional seedling-cultivating method, improves rudiment gesture 13.2%, improves germination rate 20.1%.
The above; be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; the change that can expect easily or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should define with claim is as the criterion.
Claims (3)
1. utilize hydrogen peroxide to improve fragrant camphor tree seed germination rate technology, mainly comprise the following steps: (1), seed collecting and the production of hybrid seeds, (2), earthing of seeds process, (3), presprouting of seeds process, (4), seeding in nursery bed, (5), seedling management, (6), to transplant seedlings and field planting, (7), nursery stock management and maintenance, it is characterized in that, described presprouting of seeds process comprises the following steps: first by the emerge in worm water 2h of seed with 50 DEG C during vernalization, make the moisture of neutron absorption abundance, put into container again, by the H of the standard 1%-5% of 1000ml-1200ml/kg
2o
2process, temperature is 25-28 DEG C, and humidity is 55-65%, cleans afterwards, for sowing with clear water.
2. utilize hydrogen peroxide to improve fragrant camphor tree seed germination rate technology as claimed in claim 1, it is characterized in that, described seeding in nursery bed comprises the following steps: sanding gravel and fine sand under nursery pond, upper strata paving 15cm thickness seedling medium, seedling medium presses fertilizer: the standard of vermiculite=3:1, the wide 1.2-1.5m in general seedbed, length is how many depending on seed; Seed drilling is watered permeable the previous day, and first on furrow face, first open a ditch during sowing, minor groove width is generally 10-12cm, the degree of depth is 4-5cm, and bar ditch spacing 15-20cm, carries out seed program request, plant spacing and be generally 4-5cm, often row broadcasts 30-40 grain, after every bar ditch seed finishes, and earthing leveling also Shi Dang Hong water, with lose seed not ponding be advisable, use with good conditionsi ground temperature heating wire, maintenances substrate temperature is 30-35 DEG C, the initial stage for being incubated, moisturizing can take Small plastic shed or lid straw screen or mat.
3. utilize hydrogen peroxide to improve fragrant camphor tree seed germination rate technology as claimed in claim 1, it is characterized in that, described H
2o
2concentration is 5%, and the processing time is 4-6h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510635768.0A CN105230181B (en) | 2015-09-30 | 2015-09-30 | The method that cinnamomum camphora seed germination rate is improved using hydrogen peroxide |
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| CN201510635768.0A CN105230181B (en) | 2015-09-30 | 2015-09-30 | The method that cinnamomum camphora seed germination rate is improved using hydrogen peroxide |
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| CN105230181B CN105230181B (en) | 2017-07-28 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106358734A (en) * | 2016-09-13 | 2017-02-01 | 山西省农业科学院高粱研究所 | Method for increasing germination rate of sorghum seeds |
| CN106688778A (en) * | 2016-11-28 | 2017-05-24 | 全椒凤巢植保专业合作社 | Method of cultivating Cinnamomum camphora seedlings |
| CN113367018A (en) * | 2021-07-07 | 2021-09-10 | 河南农业大学 | Method for rapidly and efficiently improving survival rate of nursery stock afforestation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
| CN103718693A (en) * | 2012-10-15 | 2014-04-16 | 重庆市南川区南双科技有限公司 | Germination method of cinnamomum camphora seeds |
| US20140130409A1 (en) * | 2012-11-14 | 2014-05-15 | Jennifer Vollmer | Method to Arrest Growth of Invasive Species' Seeds While in Non-Natural Environment Prior to Transport |
| CN104429511A (en) * | 2014-11-19 | 2015-03-25 | 安顺市西秀区春实绿化苗木有限公司 | Seedling cultivation method of camphor trees |
-
2015
- 2015-09-30 CN CN201510635768.0A patent/CN105230181B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
| CN103718693A (en) * | 2012-10-15 | 2014-04-16 | 重庆市南川区南双科技有限公司 | Germination method of cinnamomum camphora seeds |
| US20140130409A1 (en) * | 2012-11-14 | 2014-05-15 | Jennifer Vollmer | Method to Arrest Growth of Invasive Species' Seeds While in Non-Natural Environment Prior to Transport |
| CN104429511A (en) * | 2014-11-19 | 2015-03-25 | 安顺市西秀区春实绿化苗木有限公司 | Seedling cultivation method of camphor trees |
Non-Patent Citations (2)
| Title |
|---|
| 王三根: "《高级植物生理教程》", 31 August 2010, 西南师范大学出版社 * |
| 郭江雪: "香樟育苗技术", 《现代农业科技》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106358734A (en) * | 2016-09-13 | 2017-02-01 | 山西省农业科学院高粱研究所 | Method for increasing germination rate of sorghum seeds |
| CN106358734B (en) * | 2016-09-13 | 2019-12-17 | 山西省农业科学院高粱研究所 | Method for improving germination rate of sorghum seeds |
| CN106688778A (en) * | 2016-11-28 | 2017-05-24 | 全椒凤巢植保专业合作社 | Method of cultivating Cinnamomum camphora seedlings |
| CN113367018A (en) * | 2021-07-07 | 2021-09-10 | 河南农业大学 | Method for rapidly and efficiently improving survival rate of nursery stock afforestation |
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| CN105230181B (en) | 2017-07-28 |
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Granted publication date: 20170728 Termination date: 20190930 |