CN102487627A - Method for effectively breaking dormancy of Paris polyphylla seeds - Google Patents

Method for effectively breaking dormancy of Paris polyphylla seeds Download PDF

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Publication number
CN102487627A
CN102487627A CN2011104085217A CN201110408521A CN102487627A CN 102487627 A CN102487627 A CN 102487627A CN 2011104085217 A CN2011104085217 A CN 2011104085217A CN 201110408521 A CN201110408521 A CN 201110408521A CN 102487627 A CN102487627 A CN 102487627A
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Prior art keywords
paris polyphylla
seed
hydrogen peroxide
remove
exosper
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CN2011104085217A
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CN102487627B (en
Inventor
田梦良
王跃华
刘勇
周杰
江明殊
张珏
李文光
陈燕
张红玉
薛惠玲
藏薇
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Sichuan Agricultural University
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Sichuan Agricultural University
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Abstract

The invention discloses a method for effectively breaking the dormancy of Paris polyphylla seeds. By adopting a treatment way of combining hydrogen peroxide and the Gibberellins solution with suitable concentrations, the dormancy of the Paris polyphylla seeds is effectively broken, the after ripening period of the seeds is shortened, the germination time of about 2 years for the Paris polyphylla seeds in natural conditions is shortened to about 3 months, and the cultivation time of the Paris polyphylla seeds is greatly shortened.

Description

The seed dormant method of a kind of effective releasing Paris polyphylla
Technical field
The present invention relates to the seed dormant method of a kind of effective releasing Paris polyphylla.
Background technology
The Paris polyphylla plant is subordinate to the Liliaceae Paris, the primary raw material that is that multiple Chinese patent drug such as Yunnan baiyao, palace blood are peaceful, the heat poison is clear etc.Along with constantly widening of its purposes, the demand of Paris polyphylla resource also increases sharply.Show that according to statistics at present, available Paris polyphylla resource 80% has been developed totally nearly, remaining 20% resource possibly kept 3~5 years according to present international and domestic demand analysis.Therefore, the plantation of carrying out the Paris polyphylla medicinal plant on a large scale has very important meaning.
The subject matter that the plantation of restriction Paris polyphylla plant scale is at present produced is the shortage of Paris polyphylla seedling; Because the embryo of Paris polyphylla plant seed when fruit maturation is hypoplasia still; Plumule, plumular axis, radicle and cotyledon are not all also grown and are formed in its embryo; The characteristic of form after-ripening such as and endosperm is hard, secundine top layer keratinization and physiological dormancy makes the Paris polyphylla seed behind conventional field sowing, need could sprout experience one summer of two winters usually and emerges; The needed time of seed germination is longer, is typical " biennial seed "; And under nature, even it is also lower to have experienced its emergence rate of Paris polyphylla seed of long-time dormancy.
Patent that at present existing people has just broken the seed dormant art applications of Paris polyphylla is that 98118955.5 patent application document discloses a kind of " Yunnan Rhizoma Paridis seed sprouting and quick propagating technology thereof " like number of patent application, and this technology is earlier with the gibberellin immersion of Paris polyphylla seed with 20-80ppm; And then adopt 4 ℃ alternately to cultivate with 22 ℃ of alternating temperatures; In medium, add hormone and Yunnan Rhizoma Paridis oligosaccharide simultaneously, so that seed the earliest can be sprouting in 2 months, though this technology can effectively be broken the Paris polyphylla Seed Dormancy phase; Shorten the seed germination time; But the alternating temperature complicated operating process, and need at last to insert to contain in the medium of hormone and Yunnan Rhizoma Paridis oligosaccharide, production cost is high.
Summary of the invention
The object of the present invention is to provide a kind of simple to operate, production cost is lower can effectively remove the seed dormant method of Paris polyphylla, this method can shorten the time of seed morphology after-ripening and physiological dormancy greatly, and the Paris polyphylla seed is sprouted within a short period of time.
For achieving the above object, the solution that the present invention adopts comprises the following steps:
(1) remove exosper: the seed of gathering when being chosen in Paris polyphylla fruit maturation, pericarp cracking, fresh Paris polyphylla seed is mixed with wet sand, in time remove the exosper of Paris polyphylla seed with the mode of rubbing each other, and clean with flushing with clean water;
(2) hydrogen peroxide solution is handled: the Paris polyphylla seed that will remove exosper with the filter paper suck dry moisture after, put into 1~3% hydrogen peroxide solution and soak 5~10h, immersion process carries out under 0~8 ℃ of shading condition;
(3) gibberellin treatment: the Paris polyphylla seed after the hydrogen peroxide solution processing is blotted the surface with filter paper earlier; Placing 12~24h at 18~25 ℃ then dries; Seed after will drying is again put into the Gibberellins solution of 200~400mg/L and is soaked 12~48h, and immersion process carries out under 18~25 ℃ of shading conditions;
(4) sprout cultivation: take out the seed after in Gibberellins solution, soaking; Behind the filter paper suck dry moisture; Be placed on the super-clean bench earlier ethanol disinfection 30s with 70%~75%; Again with 0.1%~0.2% mercuric chloride sterilization, 4~10min, be inoculated in the 1/2MS medium with after the sterile water wash three times then, be that 1000~1800lx, illumination every day 8~12h, temperature cultivate under 16~25 ℃ the condition of culture in intensity of illumination.
The present invention is directed to the design feature that the Paris polyphylla seed is had; At first will suppress the exosper of Paris polyphylla seed germination removes totally in time; Then handle higher, the scleroid secundine of Paris polyphylla seed keratinization degree, to strengthen the permeability of secundine with the hydrogen peroxide solution of suitable concentration; And then seed put into the Gibberellins solution immersion treatment of suitable concentration; With the morphogenesis of quickening each organ in the Paris polyphylla embryo with reach maturity; Promote further that simultaneously the reserve substance endosperm is converted into state capable of using in the Paris polyphylla seed; Supply with the needs that blastocyte division of seed germination initial stage and organ form in time, accomplish the form and the physiology after-ripening of Paris polyphylla seed, finally break the Paris polyphylla Seed Dormancy effectively; Make the Paris polyphylla seed need under the natural conditions could sprout in about 2 years and shorten to about 3 months time of the fastest need, shortened the sprout time of Paris polyphylla seed widely.In addition, the inventive method is simple to operate, production cost is lower, is easy to promote.
Below in conjunction with embodiment the present invention is explained further details, but content of the present invention is not limited in this.
Embodiment
Embodiment 1
(1) remove exosper: the seed of gathering when being chosen in Paris polyphylla fruit maturation, pericarp cracking, fresh Paris polyphylla seed is mixed with wet sand, in time remove the exosper of red succulence outside the Paris polyphylla seed with the mode of rubbing each other, and clean with flushing with clean water;
(2) hydrogen peroxide solution is handled: the Paris polyphylla seed that will remove exosper with the filter paper suck dry moisture after, put into 2% hydrogen peroxide solution and soak 8h, whole immersion process carries out under 4 ℃ of shading conditions;
(3) gibberellin treatment: after the Paris polyphylla seed after hydrogen peroxide solution handled blots surface moisture with filter paper earlier; Placing 18h at 20 ℃ then dries; Seed after will drying is again put into the Gibberellins solution of 300mg/L and is soaked 36h, and immersion process carries out under 20 ℃ of shading conditions;
(4) sprout to cultivate: take out the seed after in Gibberellins solution, soaking, with the filter paper suck dry moisture after, be placed on elder generation on the super-clean bench with 70% ethanol disinfection 30s; Again with 0.1% mercuric chloride sterilization 8min; Being inoculated in the 1/2MS medium with after the sterile water wash three times then, is that 1500lx, illumination every day 10h, temperature cultivate in 20 ℃ the culturing room in intensity of illumination, cultivates after about 3 months; The Paris polyphylla seed grows radicle, begins to sprout.
Embodiment 2
Change the concentration of hydrogen peroxide solution in embodiment 1 step (2) into employing 1%, other step is with embodiment 1, and the Paris polyphylla seed grows radicle after cultivating 5 months, begin to sprout.
Embodiment 3
Change the concentration of Gibberellins solution in embodiment 1 step (3) into 400mg/L, other step is with embodiment 1, and the Paris polyphylla seed grows radicle after cultivating 4 months, begin to sprout.

Claims (1)

1. effectively remove the seed dormant method of Paris polyphylla for one kind, it is characterized in that comprising the steps:
(1) remove exosper: the seed of gathering when being chosen in Paris polyphylla fruit maturation, pericarp cracking, fresh Paris polyphylla seed is mixed with wet sand, in time remove the exosper of Paris polyphylla seed with the mode of rubbing each other, and clean with flushing with clean water;
(2) hydrogen peroxide solution is handled: the Paris polyphylla seed that will remove exosper with the filter paper suck dry moisture after, put into 1~3% hydrogen peroxide solution and soak 5~10h, immersion process carries out under 0~8 ℃ of shading condition;
(3) gibberellin treatment: after the Paris polyphylla seed after hydrogen peroxide solution handled blots surface moisture with filter paper earlier; Placing 12~24h at 18~25 ℃ then dries; Seed after will drying is again put into the Gibberellins solution of 200~400mg/L and is soaked 12~48h, and immersion process carries out under 18~25 ℃ of shading conditions;
(4) sprout cultivation: take out the seed after in Gibberellins solution, soaking; Behind the filter paper suck dry moisture; Be placed on the super-clean bench earlier ethanol disinfection 30s,, be inoculated in the 1/2MS medium with after the sterile water wash three times then again with 0.1~0.2% mercuric chloride, the 4~10min that sterilizes with 70~75%; In intensity of illumination is 1000~1800lx, illumination every day 8~12h, and temperature is to cultivate under 16~25 ℃ the condition of culture.
CN2011104085217A 2011-12-09 2011-12-09 Method for effectively breaking dormancy of Paris polyphylla seeds Expired - Fee Related CN102487627B (en)

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Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103081607A (en) * 2013-02-26 2013-05-08 成都大学 Preservation method of horseradish seeds
CN103222362A (en) * 2013-04-27 2013-07-31 云南农业大学 Method for improving paris polyphylla var. yunnanensis seed germination rate
CN103621557A (en) * 2013-12-20 2014-03-12 贵州大学 Special treatment agent for polyphylla seed germination
CN104472062A (en) * 2014-12-31 2015-04-01 包头医学院 Method for promoting cymbariae seeds to germinate
CN104541655A (en) * 2013-10-15 2015-04-29 云南白药集团中药材优质种源繁育有限责任公司 Method for breaking paris polyphylla seed dormancy
CN104620718A (en) * 2015-02-13 2015-05-20 湖南农业大学 Method for breaking dormancy of digitaria sanguinalis seeds
CN105230181A (en) * 2015-09-30 2016-01-13 宿迁学院 Technology utilizing hydrogen peroxide to improve germination rate of cinnamomum camphora
CN105309180A (en) * 2015-08-06 2016-02-10 董唯书 A rhizoma paridis planting method
CN105453752A (en) * 2015-11-23 2016-04-06 文山壮族苗族自治州农业科学院 Method for promoting rapid germination of rhizoma paridis seeds
CN106069093A (en) * 2016-06-29 2016-11-09 中国科学院西双版纳热带植物园 A kind of polygerm breeding method of Rhizoma Paridis
CN106105481A (en) * 2016-07-01 2016-11-16 文山苗乡三七科技有限公司 A kind of germination accelerating method of Rhizoma Paridis seed
CN106342598A (en) * 2016-08-22 2017-01-25 兰坪易慧农业开发有限公司 Planting method promoting growth of paris polyphylla
CN106358500A (en) * 2016-08-29 2017-02-01 青川县蜀道地中药材发展有限公司 Paris polyphylla var Chinensis seed germination method
CN106508349A (en) * 2016-10-21 2017-03-22 兴义市庆庆种植有限责任公司 Rapid cultivation method for polyphylla seedlings
CN107006162A (en) * 2017-02-24 2017-08-04 安徽榈源农业科技有限公司 A kind of method for releasing fresh vetch seed dormancy
CN107027624A (en) * 2017-04-28 2017-08-11 云南农业大学 A kind of method for obtaining polygerm Yunnan Paris polyphylla seedling
CN107980634A (en) * 2017-12-15 2018-05-04 贵州大学 A kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation
CN108040530A (en) * 2017-12-01 2018-05-18 亳州学院 The wild Paris polyphylla seed dormancy in Dabie Mountain breaks method
CN108156880A (en) * 2018-01-11 2018-06-15 四川农业大学 A kind of method for culturing seedlings for improving greenleaf piptanthus seed seeding ratio
CN108401826A (en) * 2018-06-04 2018-08-17 安徽俊祺生物科技有限公司 A kind of seedling-raising technique of Paris polyphylla
CN108811584A (en) * 2018-06-26 2018-11-16 中国科学院成都生物研究所 A method of promoting Paris polyphylla seed fast germination
CN108901237A (en) * 2018-06-06 2018-11-30 武汉叶动力生物科技有限公司 A method of quickly breaking Paris polyphylla top-wall effect
CN110140472A (en) * 2019-05-23 2019-08-20 中国林业科学研究院亚热带林业实验中心 A method of promote paris polyphylla seed to sprout
CN111869362A (en) * 2020-08-01 2020-11-03 刘泽洋 Method for breaking dormancy of hard seeds
CN111937525A (en) * 2020-08-11 2020-11-17 湖南农佳好伙计农业科技发展有限公司 Paris polyphylla seed sand storage, temperature change and medicament treatment germination accelerating method

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CN1210663A (en) * 1998-09-08 1999-03-17 中国科学院昆明植物研究所 Seed sprouting and quick propagating technology of Yunnan Rhizoma Paridis
CN1602656A (en) * 2004-11-01 2005-04-06 成都中康光大药业有限公司 Wild Paris chinensis Franch artificial domestication grow seedling and cultivation method
US20060147569A1 (en) * 2005-01-03 2006-07-06 Hsiang-Fu Kung Anti-depression formulations
CN101278612A (en) * 2008-05-20 2008-10-08 云南大学 Method for breaking seed dormancy of Paris polyphylla var. yunnanensis
CN101796899A (en) * 2009-05-14 2010-08-11 北京师范大学 Method for sprouting seeds of polyphylla Smith var.ynynanensis (Franch.) Hand-Mazz

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1210663A (en) * 1998-09-08 1999-03-17 中国科学院昆明植物研究所 Seed sprouting and quick propagating technology of Yunnan Rhizoma Paridis
CN1602656A (en) * 2004-11-01 2005-04-06 成都中康光大药业有限公司 Wild Paris chinensis Franch artificial domestication grow seedling and cultivation method
US20060147569A1 (en) * 2005-01-03 2006-07-06 Hsiang-Fu Kung Anti-depression formulations
CN101278612A (en) * 2008-05-20 2008-10-08 云南大学 Method for breaking seed dormancy of Paris polyphylla var. yunnanensis
CN101796899A (en) * 2009-05-14 2010-08-11 北京师范大学 Method for sprouting seeds of polyphylla Smith var.ynynanensis (Franch.) Hand-Mazz

Cited By (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103081607A (en) * 2013-02-26 2013-05-08 成都大学 Preservation method of horseradish seeds
CN103081607B (en) * 2013-02-26 2014-12-03 成都大学 Preservation method of horseradish seeds
CN103222362A (en) * 2013-04-27 2013-07-31 云南农业大学 Method for improving paris polyphylla var. yunnanensis seed germination rate
CN104541655A (en) * 2013-10-15 2015-04-29 云南白药集团中药材优质种源繁育有限责任公司 Method for breaking paris polyphylla seed dormancy
CN103621557A (en) * 2013-12-20 2014-03-12 贵州大学 Special treatment agent for polyphylla seed germination
CN104472062A (en) * 2014-12-31 2015-04-01 包头医学院 Method for promoting cymbariae seeds to germinate
CN104620718A (en) * 2015-02-13 2015-05-20 湖南农业大学 Method for breaking dormancy of digitaria sanguinalis seeds
CN104620718B (en) * 2015-02-13 2016-08-24 湖南农业大学 One breaks the seed dormant method of Herba Digitariae
CN105309180A (en) * 2015-08-06 2016-02-10 董唯书 A rhizoma paridis planting method
CN105230181A (en) * 2015-09-30 2016-01-13 宿迁学院 Technology utilizing hydrogen peroxide to improve germination rate of cinnamomum camphora
CN105453752A (en) * 2015-11-23 2016-04-06 文山壮族苗族自治州农业科学院 Method for promoting rapid germination of rhizoma paridis seeds
CN106069093A (en) * 2016-06-29 2016-11-09 中国科学院西双版纳热带植物园 A kind of polygerm breeding method of Rhizoma Paridis
CN106105481A (en) * 2016-07-01 2016-11-16 文山苗乡三七科技有限公司 A kind of germination accelerating method of Rhizoma Paridis seed
CN106342598A (en) * 2016-08-22 2017-01-25 兰坪易慧农业开发有限公司 Planting method promoting growth of paris polyphylla
CN106358500A (en) * 2016-08-29 2017-02-01 青川县蜀道地中药材发展有限公司 Paris polyphylla var Chinensis seed germination method
CN106508349A (en) * 2016-10-21 2017-03-22 兴义市庆庆种植有限责任公司 Rapid cultivation method for polyphylla seedlings
CN107006162A (en) * 2017-02-24 2017-08-04 安徽榈源农业科技有限公司 A kind of method for releasing fresh vetch seed dormancy
CN107027624A (en) * 2017-04-28 2017-08-11 云南农业大学 A kind of method for obtaining polygerm Yunnan Paris polyphylla seedling
CN108040530A (en) * 2017-12-01 2018-05-18 亳州学院 The wild Paris polyphylla seed dormancy in Dabie Mountain breaks method
CN107980634A (en) * 2017-12-15 2018-05-04 贵州大学 A kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation
CN108156880A (en) * 2018-01-11 2018-06-15 四川农业大学 A kind of method for culturing seedlings for improving greenleaf piptanthus seed seeding ratio
CN108401826A (en) * 2018-06-04 2018-08-17 安徽俊祺生物科技有限公司 A kind of seedling-raising technique of Paris polyphylla
CN108901237A (en) * 2018-06-06 2018-11-30 武汉叶动力生物科技有限公司 A method of quickly breaking Paris polyphylla top-wall effect
CN108811584A (en) * 2018-06-26 2018-11-16 中国科学院成都生物研究所 A method of promoting Paris polyphylla seed fast germination
CN110140472A (en) * 2019-05-23 2019-08-20 中国林业科学研究院亚热带林业实验中心 A method of promote paris polyphylla seed to sprout
CN110140472B (en) * 2019-05-23 2021-03-23 中国林业科学研究院亚热带林业实验中心 Method for promoting germination of paris polyphylla seeds
CN111869362A (en) * 2020-08-01 2020-11-03 刘泽洋 Method for breaking dormancy of hard seeds
CN111869362B (en) * 2020-08-01 2022-05-03 黑龙江省仓皓农业发展有限公司 Method for improving seed coat permeability and seed embryo respiration of hard seed
CN111937525A (en) * 2020-08-11 2020-11-17 湖南农佳好伙计农业科技发展有限公司 Paris polyphylla seed sand storage, temperature change and medicament treatment germination accelerating method

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