CN103081607B - Preservation method of horseradish seeds - Google Patents
Preservation method of horseradish seeds Download PDFInfo
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- CN103081607B CN103081607B CN201310059779.XA CN201310059779A CN103081607B CN 103081607 B CN103081607 B CN 103081607B CN 201310059779 A CN201310059779 A CN 201310059779A CN 103081607 B CN103081607 B CN 103081607B
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- seed
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- bag
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- 238000004321 preservation Methods 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title claims abstract description 15
- 240000003291 Armoracia rusticana Species 0.000 title abstract description 13
- 235000011330 Armoracia rusticana Nutrition 0.000 title abstract description 10
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 23
- 238000007710 freezing Methods 0.000 claims abstract description 5
- 230000008014 freezing Effects 0.000 claims abstract description 5
- 235000000760 Wasabia japonica Nutrition 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 11
- 239000004677 Nylon Substances 0.000 claims description 10
- 229920001778 nylon Polymers 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 229920006395 saturated elastomer Polymers 0.000 claims description 7
- 235000002639 sodium chloride Nutrition 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 6
- 229930182764 Polyoxin Natural products 0.000 claims description 6
- YEBIHIICWDDQOL-YBHNRIQQSA-N polyoxin Polymers O[C@@H]1[C@H](O)[C@@H](C(C=O)N)O[C@H]1N1C(=O)NC(=O)C(C(O)=O)=C1 YEBIHIICWDDQOL-YBHNRIQQSA-N 0.000 claims description 6
- 230000007613 environmental effect Effects 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 239000008223 sterile water Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 241001293164 Eutrema japonicum Species 0.000 claims 4
- 230000035784 germination Effects 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 3
- 230000007774 longterm Effects 0.000 abstract description 3
- 244000195452 Wasabia japonica Species 0.000 description 29
- 239000004576 sand Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- -1 isosulfocyanate compound Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 240000003945 Cochlearia officinalis Species 0.000 description 1
- 235000007463 Cochlearia officinalis Nutrition 0.000 description 1
- 241000390128 Eutrema Species 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- ZPUCINDJVBIVPJ-LJISPDSOSA-N cocaine Chemical compound O([C@H]1C[C@@H]2CC[C@@H](N2C)[C@H]1C(=O)OC)C(=O)C1=CC=CC=C1 ZPUCINDJVBIVPJ-LJISPDSOSA-N 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000018927 edible plant Nutrition 0.000 description 1
- 229910001254 electrum Inorganic materials 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 239000010940 green gold Substances 0.000 description 1
- 235000019604 hot taste sensations Nutrition 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 235000019508 mustard seed Nutrition 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- WCEMVDXZPVFHAD-UHFFFAOYSA-N prop-2-enoyl isothiocyanate Chemical compound C=CC(=O)N=C=S WCEMVDXZPVFHAD-UHFFFAOYSA-N 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000010129 solution processing Methods 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 108010058651 thioglucosidase Proteins 0.000 description 1
Landscapes
- Storage Of Fruits Or Vegetables (AREA)
Abstract
The invention discloses a preservation method of horseradish seeds, and the method comprises the following steps of: fruit drying-quick hulling-seed sterilization-seed air-drying-seed freezing and preservation. The method disclosed by the invention not only can be used for realizing the long-term preservation of the horseradish seeds but also ensures that the horseradish seeds can not be mildewed within the preservation period, and retains the high activity and the germination rate.
Description
Technical field
The present invention relates to a kind of method for preserving of wasabi seed.
Background technology
Horseradish (Wasabi japonica Matsum) has another name called scurvy grass, mountain dish, is the perennial half cloudy raw herbaceous plant of Cruciferae Eutrema.
The material that contains a kind of mustard seed glycoside in horseradish plant, in the time that plant corpus is grated, this material is hydrolyzed and forms isosulfocyanate compound under the effect of myrosinase, wherein especially the highest with acrylic isothiocyanate content, accounts for 89%～94%; In addition, horseradish plant also contains the trace element of a large amount of amino acid and various human body needs.The amino acid and the trace element that contain abundant isosulfocyanate compound and a large amount of human body needs due to horseradish, therefore horseradish not only has strong hot taste and the special pungent taste of perfume (or spice), but also there is the multiple effects such as immunological regulation, antibacterial, anticancer, anti-oxidant, platelet aggregation-against, be the very rare and precious medicinal and edible plant of generally acknowledging in the world, be called as " green gold ".
The breeding of horseradish plant adopts division propagation and seminal propagation more.Division propagation not only exists many for nourishing and generating the serious problem of kind of sexual involution, and reproduction coefficient is low, seedling carries disease germs seriously, very easily causes disease large area to occur.Adopt seed to carry out horseradish sapling multiplication and have that speed is fast, quantity is many, can reduce the advantages such as disease generation.But because wasabi seed has recalcitrant seed, dehydration too much very easily causes the forfeiture of its vigor, and therefore wasabi seed is difficult to long-term preservation, must take low temperature under certain damp condition to preserve.Before this, Cai Guangze is in the research of " collection, processing and the storage of wasabi seed ", adopt and pack in nylon net bag after under fresh fruit, then mesh bag being placed in to mountain spring flowing water soaks, need be through 25 days, pericarp could all rot, and it is longer that therefore the method obtains the time that clean wasabi seed spends.The people such as Mo Huiji, in the research of " blossom and bear fruit characteristic and the seedling growing process of reserving seed for planting of Japanese horseradish ", adopt the ratio that the wasabi seed drying after sterilization is added to 3 parts of fine sands in 1 part of seed to mix, and then bury in the high mountain limit of height above sea level 1300 m left and right.Dark approximately 1 m in cave that buries seed, cobble and the rough sand of 30～40 cm is put in the bottom in cave, and the seed that is mixed with fine sand is put into the clean fine sand covering of rear use, and fine sand will be taped against ground always, and this storage link is more, step is also more loaded down with trivial details.
Summary of the invention
The object of the present invention is to provide the method for preserving of short, the simple wasabi seed of preservation process of a kind of processing time, the method can not only realize the long-term preservation of wasabi seed, and can also make wasabi seed in storage life, be difficult for going mouldy, and keep higher vigor and germination rate.
The method for preserving of wasabi seed provided by the invention comprises the following steps:
(1) fruit is dry: the fruit of gathering is sub-packed in nylon net bag, is placed in and is not subject to that direct sunlight, ventilation condition are good, temperature is that the environment of 18～25 DEG C dries in the shade, frequently stir therebetween;
(2) fast except pericarp: dry in the shade while easily separating with seed to pericarp until fruit, fruit is put into hydrogen peroxide solution to be soaked, then use flowing water continuous flushing, constantly remove the pericarp residue coming off in bag therebetween, after pericarp separates completely with seed, remove pericarp residue, obtain clean wasabi seed;
(3) seed disinfection: the wasabi seed of acquisition is blotted after surperficial moisture with filter paper, put into Polyoxin solution and soak;
(4) seed dries: the wasabi seed after sterilization, with after sterile water wash, is placed under the environmental condition of 15～25 DEG C and is dried in the shade, measure the variation of seed moisture content;
(5) the freezing preservation of seed: in the time that the water content that records seed is 15～35%, packed in the nylon net bag of having sterilized, put into again the refrigerator-freezer that is built-in with multilayer shelf, every bag of seed is laid on shelf equably, refrigerator-freezer temperature remains on 3～8 DEG C, in refrigerator-freezer, be placed with saturated aqueous common salt, the bottom shelf of described multilayer shelf should be apart from the certain distance in refrigerator-freezer bottom.
Further, in step (2), hydrogen peroxide solution concentration is 1%～5%, and soak time is 4～6 hours.
Further, in step (3), the concentration of Polyoxin solution is 0.1%～0.5%, and soak time is 2～8 hours.
Further, in step (5), every layer of shelf is at a distance of 5～10 centimetres, and bottom shelf is apart from 10～15 centimetres of refrigerator-freezer bottoms.
Tool of the present invention has the following advantages:
(1) owing to adopting, horseradish fruit is dried in the shade while easily separating with seed to pericarp, then use certain density hydrogen peroxide solution processing, therefore can remove fast pericarp;
(2) because wasabi seed has adopted the disinfection of certain density Polyoxin solution putting into before refrigerator-freezer, therefore can effectively prevent that seed from going mouldy in preserving process, and make seed after preservation, still keep high germination rate;
(3) owing to adopting, every bag of seed is laid on the refrigerator-freezer shelf that keeps certain distance equably, and saturated aqueous common salt is placed in employing in refrigerator-freezer, thereby moisture stable can make seed preserve time, thereby can guarantee that seed still maintains higher germination rate after long-time preservation in refrigerator-freezer.
Embodiment
Embodiment 1
(1) fruit is dry: the fruit of gathering is sub-packed in nylon net bag, every bag of volume of fruits accounts for 1/5～1/3 of mesh bag volume, then be placed in and be not subject to that direct sunlight, ventilation condition are good, temperature is that the environment of 18～25 DEG C dries in the shade 3～6 days, stir 2-4 every day;
(2) fast except pericarp: dry in the shade while easily separating with seed to pericarp until fruit, fruit being put into 3% hydrogen peroxide solution soaks 5 hours, then use flowing water continuous flushing, constantly remove the pericarp residue coming off in bag therebetween, after pericarp separates completely with seed, further clean out pericarp residue, obtain clean wasabi seed;
(3) seed disinfection: the wasabi seed of acquisition is blotted after surperficial moisture with filter paper, and the Polyoxin solution of putting into 0.3 % soaks 6 hours;
(4) seed dries: the wasabi seed after sterilization, with after sterile water wash 2～5 times, is placed under the environmental condition of 22 DEG C and is dried in the shade, measure the variation of seed moisture content;
(5) the freezing preservation of seed: in the time that the water content that records seed is 25%, packed in the nylon net bag of having sterilized, every packed 1/3 left and right seed, then put into the refrigerator-freezer that is built-in with multilayer shelf, every bag of seed is laid on shelf equably, refrigerator-freezer temperature remains on 5 DEG C, the saturated aqueous common salt that refrigerator-freezer bottom is placed with one glass of 250mL (will add after saturated common salt water consumption, remain and in refrigerator-freezer, have saturated aqueous common salt), the bottom shelf of described multilayer shelf is apart from 12 centimetres of refrigerator-freezer bottoms, and every layer of shelf is at a distance of 8 centimetres.Wasabi seed can be preserved 1 year above and be mildewproof, and the germination rate of seed can reach 90.1%.
Embodiment 2
(1) fruit is dry: the fruit of gathering is sub-packed in nylon net bag, every bag of volume of fruits accounts for 1/5～1/3 of mesh bag volume, then be placed in and be not subject to that direct sunlight, ventilation condition are good, temperature is that the environment of 18～25 DEG C dries in the shade 3～6 days, stir 2-4 every day;
(2) fast except pericarp: dry in the shade while easily separating with seed to pericarp until fruit, fruit being put into 5% hydrogen peroxide solution soaks 4 hours, then use flowing water continuous flushing, constantly remove the pericarp residue coming off in bag therebetween, after pericarp separates completely with seed, further clean out pericarp residue, obtain clean wasabi seed;
(3) seed disinfection: the wasabi seed of acquisition is blotted after surperficial moisture with filter paper, put into 0.5% Polyoxin solution and soak 3 hours;
(4) seed dries: the wasabi seed after sterilization, with after sterile water wash 2～5 times, is placed under the environmental condition of 22 DEG C and is dried in the shade, measure the variation of seed moisture content;
(5) the freezing preservation of seed: in the time that the water content that records seed is 15%, packed in the nylon net bag of having sterilized, every packed 1/3 left and right seed, then put into the refrigerator-freezer that is built-in with multilayer shelf, every bag of seed is laid on shelf equably, and refrigerator-freezer temperature remains on 5 DEG C, and refrigerator-freezer bottom is placed with the saturated aqueous common salt of one glass of 250mL, the bottom shelf of described multilayer shelf is apart from 12 centimetres of refrigerator-freezer bottoms, and every layer of shelf is at a distance of 8 centimetres.Wasabi seed can be preserved 1 year above and be mildewproof, and the germination rate of seed is 65.4%.
Comparing embodiment 1
Embodiment 1 step (1) is changed into: the horseradish fruit of gathering is sub-packed in nylon net bag, is placed in 40 DEG C, the environmental condition Xiayang of direct sunlight dry 5 days, other steps are with embodiment 1, and wasabi seed was preserved after 1 year, and germination rate only has 30.2%.
Comparing embodiment 2
Cancel embodiment 1 step (3), other steps are with embodiment 1, and preservation is after 4 months in refrigerator-freezer for wasabi seed, and mouldy phenomenon appears in part seed, explanation thus, and seed disinfection before preservation is extremely important.
Comparing embodiment 3
Change the water content of seed in embodiment 1 step (5) into 45% by 25%, other steps are with embodiment 1, and wasabi seed has situation mouldy and that sprout in advance and produces in preserving process.
Comparing embodiment 4
In step (5), saturated aqueous common salt is not placed in refrigerator-freezer bottom, and other steps are with embodiment 1, and wasabi seed is 73.1% at the germination rate of preserving after a year.
Claims (1)
1. a method for preserving for wasabi seed, is characterized in that comprising the steps:
(1) fruit is dry: the fruit of gathering is sub-packed in nylon net bag, every bag of volume of fruits accounts for 1/5～1/3 of mesh bag volume, then be placed in and be not subject to that direct sunlight, ventilation condition are good, temperature is that the environment of 18～25 DEG C dries in the shade 3～6 days, stir 2-4 every day;
(2) fast except pericarp: dry in the shade while easily separating with seed to pericarp until fruit, fruit being put into 3% hydrogen peroxide solution soaks 5 hours, then use flowing water continuous flushing, constantly remove the pericarp residue coming off in bag therebetween, after pericarp separates completely with seed, further clean out pericarp residue, obtain clean wasabi seed;
(3) seed disinfection: the wasabi seed of acquisition is blotted after surperficial moisture with filter paper, put into 0.3% Polyoxin solution and soak 6 hours;
(4) seed dries: the wasabi seed after sterilization, with after sterile water wash 2～5 times, is placed under the environmental condition of 22 DEG C and is dried in the shade, measure the variation of seed moisture content;
(5) the freezing preservation of seed: in the time that the water content that records seed is 25%, packed in the nylon net bag of having sterilized, every packed 1/3 left and right seed, then put into the refrigerator-freezer that is built-in with multilayer shelf, every bag of seed is laid on shelf equably, and refrigerator-freezer temperature remains on 5 DEG C, and refrigerator-freezer bottom is placed with the saturated aqueous common salt of one glass of 250mL, the bottom shelf of described multilayer shelf is apart from 12 centimetres of refrigerator-freezer bottoms, and every layer of shelf is at a distance of 8 centimetres.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310059779.XA CN103081607B (en) | 2013-02-26 | 2013-02-26 | Preservation method of horseradish seeds |
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| CN201310059779.XA CN103081607B (en) | 2013-02-26 | 2013-02-26 | Preservation method of horseradish seeds |
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| CN103081607A CN103081607A (en) | 2013-05-08 |
| CN103081607B true CN103081607B (en) | 2014-12-03 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103829347B (en) * | 2014-03-05 | 2015-11-25 | 大姚家和天然食品开发有限责任公司 | A kind of antistaling process of Chinese prickly ash |
| CN111466285B (en) * | 2020-04-20 | 2022-01-11 | 成都大学 | Method for planting horseradish plants in water area environment |
| CN112514751B (en) * | 2020-11-30 | 2022-06-17 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Long-term storage and recovery method of cassava crossbreeding capsule |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010090517A1 (en) * | 2009-02-03 | 2010-08-12 | Monsanto Holland B.V. | Enriching the seed quality of a batch of seeds |
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
-
2013
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010090517A1 (en) * | 2009-02-03 | 2010-08-12 | Monsanto Holland B.V. | Enriching the seed quality of a batch of seeds |
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
Non-Patent Citations (8)
| Title |
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| 吴明根 等.山葵生物生态特性的研究.《延边大学农学学报》.2000,第22卷(第3期),第211-215页. * |
| 吴震 等.山葵( Wasabi japonica) 种子发芽特性的研究.《园艺学报》.2003,第30卷(第3期),第287-290页. * |
| 山葵( Wasabi japonica) 种子发芽特性的研究;吴震 等;《园艺学报》;20030830;第30卷(第3期);第287-290页 * |
| 山葵生物生态特性的研究;吴明根 等;《延边大学农学学报》;20000930;第22卷(第3期);第211-215页 * |
| 山葵种子的采集、处理及贮藏;蔡光泽;《种子》;20021230(第6期);第106页 * |
| 日本山葵的开花结实特性及留种育苗技术;莫慧机 等;《广东农业科学》;20041025(第5期);第23-25页 * |
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