CN103081607A - Preservation method of horseradish seeds - Google Patents
Preservation method of horseradish seeds Download PDFInfo
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- CN103081607A CN103081607A CN201310059779XA CN201310059779A CN103081607A CN 103081607 A CN103081607 A CN 103081607A CN 201310059779X A CN201310059779X A CN 201310059779XA CN 201310059779 A CN201310059779 A CN 201310059779A CN 103081607 A CN103081607 A CN 103081607A
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000004321 preservation Methods 0.000 title claims abstract description 16
- 240000003291 Armoracia rusticana Species 0.000 title abstract description 13
- 235000011330 Armoracia rusticana Nutrition 0.000 title abstract description 10
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 22
- 238000007710 freezing Methods 0.000 claims abstract description 5
- 230000008014 freezing Effects 0.000 claims abstract description 5
- 235000000760 Wasabia japonica Nutrition 0.000 claims description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000004677 Nylon Substances 0.000 claims description 10
- 229920001778 nylon Polymers 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 7
- 229930182764 Polyoxin Natural products 0.000 claims description 7
- YEBIHIICWDDQOL-YBHNRIQQSA-N polyoxin Polymers O[C@@H]1[C@H](O)[C@@H](C(C=O)N)O[C@H]1N1C(=O)NC(=O)C(C(O)=O)=C1 YEBIHIICWDDQOL-YBHNRIQQSA-N 0.000 claims description 7
- 229920006395 saturated elastomer Polymers 0.000 claims description 7
- 235000002639 sodium chloride Nutrition 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 230000007613 environmental effect Effects 0.000 claims description 5
- 238000012856 packing Methods 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 239000008223 sterile water Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 241001293164 Eutrema japonicum Species 0.000 claims 7
- 230000035784 germination Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 3
- 230000007774 longterm Effects 0.000 abstract 1
- 244000195452 Wasabia japonica Species 0.000 description 29
- 239000004576 sand Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000011010 flushing procedure Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- -1 isosulfocyanate compound Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 240000003945 Cochlearia officinalis Species 0.000 description 1
- 235000007463 Cochlearia officinalis Nutrition 0.000 description 1
- 241000390128 Eutrema Species 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- ZPUCINDJVBIVPJ-LJISPDSOSA-N cocaine Chemical compound O([C@H]1C[C@@H]2CC[C@@H](N2C)[C@H]1C(=O)OC)C(=O)C1=CC=CC=C1 ZPUCINDJVBIVPJ-LJISPDSOSA-N 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 235000018927 edible plant Nutrition 0.000 description 1
- 229910001254 electrum Inorganic materials 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 239000010940 green gold Substances 0.000 description 1
- 235000019604 hot taste sensations Nutrition 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 235000019508 mustard seed Nutrition 0.000 description 1
- WCEMVDXZPVFHAD-UHFFFAOYSA-N prop-2-enoyl isothiocyanate Chemical compound C=CC(=O)N=C=S WCEMVDXZPVFHAD-UHFFFAOYSA-N 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000010129 solution processing Methods 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 108010058651 thioglucosidase Proteins 0.000 description 1
Landscapes
- Storage Of Fruits Or Vegetables (AREA)
Abstract
The invention discloses a preservation method of horseradish seeds, and the method comprises the following steps of: fruit drying-quick hulling-seed sterilization-seed air-drying-seed freezing and preservation. The method disclosed by the invention not only can be used for realizing the long-term preservation of the horseradish seeds but also ensures that the horseradish seeds can not be mildewed within the preservation period, and retains the high activity and the germination rate.
Description
Technical field
The present invention relates to a kind of method for preserving of wasabi seed.
Background technology
Horseradish (Wasabi japonica Matsum) has another name called scurvy grass, mountain dish, is perennial half cloudy this plant of sward of Cruciferae Eutrema.
Contain a kind of material of mustard seed glycoside in the horseradish plant, when plant corpus was grated, this material is hydrolyzed under the effect of myrosinase and forms isosulfocyanate compound, and was wherein especially the highest with the acrylic isothiocyanate content, accounts for 89%～94%; In addition, the horseradish plant also contains the trace element of a large amount of amino acid and various human body needs.The amino acid and the trace element that contain abundant isosulfocyanate compound and a large amount of human body needs due to horseradish, therefore horseradish not only has strong hot taste and special fragrant hot flavor, but also have the multiple effects such as immunological regulation, antibiotic, anticancer, anti-oxidant, platelet aggregation-against, be the very rare and precious medicinal and edible plant of generally acknowledging in the world, be called as " green gold ".
Division propagation and seminal propagation are adopted in the breeding of horseradish plant more.Not only there is the serious problem of kind of sexual involution of nourishing and generating of many generations in division propagation, and reproduction coefficient is low, seedling carries disease germs seriously, very easily causes the generation of disease large tracts of land.Adopting seed to carry out the horseradish sapling multiplication has that speed is fast, quantity is many, can reduce the advantage such as disease generation.But because wasabi seed has stupid stubborn seed, dehydration too much very easily causes the forfeiture of its vigor, so wasabi seed is difficult to long preservation, must take under certain damp condition low temperature to preserve.Before this, Cai Guangze is in the research of " collection of wasabi seed, processing and storage ", adopt in the nylon net bag of packing into after under fresh fruit, then mesh bag being placed in mountain spring flowing water soaks, need through 25 days, pericarp could all rot, so the time that the clean wasabi seed of the method acquisition spends is longer.The people such as Mo Huiji are in the research of " blossom and bear fruit characteristic and the seedling growing process of reserving seed for planting of Japanese horseradish ", and the wasabi seed that employing will be dried after sterilizing adds the ratio mixing of 3 parts of fine sands in 1 part of seed, then bury the high mountain limit in about height above sea level 1300 m.Bury dark approximately 1 m in cave of seed, cobble and the rough sand of 30～40 cm is put in the bottom in cave, and the seed that is mixed with fine sand is put into the clean fine sand covering of rear use, and fine sand will be taped against ground always, and this storage link is more, step is also more loaded down with trivial details.
Summary of the invention
The object of the present invention is to provide the method for preserving of short, the simple wasabi seed of preservation process of a kind of processing time, the method can not only realize the long preservation of wasabi seed, and can also make wasabi seed be difficult for going mouldy, and keep higher vigor and germination rate in storage life.
The method for preserving of wasabi seed provided by the invention comprises the following steps:
(1) fruit is dry: the fruit of gathering is sub-packed in nylon net bag, is placed in and is not subjected to that direct sunlight, ventilation condition are good, temperature is that the environment of 18～25 ℃ dries in the shade, frequently stir therebetween;
(2) fast except pericarp: dry in the shade when easily separating with seed to pericarp until fruit, fruit is put into hydrogen peroxide solution to be soaked, then use the flowing water continuous flushing, constantly remove the pericarp residue that comes off in bag therebetween, until pericarp with after seed separates fully, remove the pericarp residue, namely obtain clean wasabi seed;
(3) seed disinfection: after the wasabi seed that obtains is blotted the moisture on surface with filter paper, put into Polyoxin solution and soak;
(4) seed dries: the wasabi seed after sterilizing with sterile water wash after, be placed under the environmental condition of 15～25 ℃ and dry in the shade, measure the variation of seed moisture content;
(5) the freezing preservation of seed: when the water content that records seed is 15～35%, in its nylon net bag of having sterilized of packing into, put into again the refrigerator-freezer that is built-in with the multilayer shelf, every bag of seed is tiled on shelf equably, the refrigerator-freezer temperature remains on 3～8 ℃, be placed with saturated aqueous common salt in refrigerator-freezer, the bottom shelf of described multilayer shelf should be apart from the certain distance in refrigerator-freezer bottom.
Further, in step (2), hydrogen peroxide solution concentration is 1%～5%, and soak time is 4～6 hours.
Further, in step (3), the concentration of Polyoxin solution is 0.1%～0.5%, and soak time is 2～8 hours.
Further, in step (5) every layer of shelf at a distance of 5～10 centimetres, 10～15 centimetres apart from the refrigerator-freezer bottom of bottom shelf.
The present invention has following advantage:
(1) owing to adopting, the horseradish fruit is dried in the shade when easily separate with seed to pericarp, then with certain density hydrogen peroxide solution processing, so can remove fast pericarp;
(2) adopted the disinfection of certain density Polyoxin solution due to wasabi seed before putting into refrigerator-freezer, therefore can prevent effectively that seed from going mouldy in preserving process, and make seed still keep high germination rate after preservation;
(3) owing to adopting, every bag of seed is tiled on the refrigerator-freezer shelf that keeps certain distance equably, and adopt and place saturated aqueous common salt in refrigerator-freezer, thereby moisture stable when seed is preserved, thereby can guarantee that seed still maintains higher germination rate after long-time the preservation in refrigerator-freezer.
Embodiment
Embodiment 1
(1) fruit is dry: the fruit of gathering is sub-packed in nylon net bag, every bag of volume of fruits accounts for 1/5～1/3 of mesh bag volume, then be placed in and be not subjected to that direct sunlight, ventilation condition are good, temperature is that the environment of 18～25 ℃ dried in the shade 3～6 days, stir 2-4 every day;
(2) fast except pericarp: dry in the shade when easily separating with seed to pericarp until fruit, fruit is put into 3% hydrogen peroxide solution to be soaked 5 hours, then use the flowing water continuous flushing, constantly remove the pericarp residue that comes off in bag therebetween, until pericarp with after seed separates fully, further clean out the pericarp residue, namely obtain clean wasabi seed;
(3) seed disinfection: after the wasabi seed that obtains was blotted the moisture on surface with filter paper, the Polyoxin solution of putting into 0.3 % soaked 6 hours;
(4) seed dries: wasabi seed after sterilizing with sterile water wash 2～5 times after, be placed under the environmental condition of 22 ℃ and dry in the shade, measure the variation of seed moisture content;
(5) the freezing preservation of seed: when the water content that records seed is 25%, in its nylon net bag of having sterilized of packing into, every packed 1/3 left and right seed, then put into the refrigerator-freezer that is built-in with the multilayer shelf, every bag of seed is tiled on shelf equably, the refrigerator-freezer temperature remains on 5 ℃, the saturated aqueous common salt that the refrigerator-freezer bottom is placed with one glass of 250mL (will add after the saturated common salt water consumption, remain saturated aqueous common salt is arranged in refrigerator-freezer), 12 centimetres apart from refrigerator-freezer bottom of the bottom shelf of described multilayer shelf, every layer of shelf is at a distance of 8 centimetres.Wasabi seed can be preserved more than 1 year and be mildewproof, and the Seed germination rate can reach 90.1%.
Embodiment 2
(1) fruit is dry: the fruit of gathering is sub-packed in nylon net bag, every bag of volume of fruits accounts for 1/5～1/3 of mesh bag volume, then be placed in and be not subjected to that direct sunlight, ventilation condition are good, temperature is that the environment of 18～25 ℃ dried in the shade 3～6 days, stir 2-4 every day;
(2) fast except pericarp: dry in the shade when easily separating with seed to pericarp until fruit, fruit is put into 5% hydrogen peroxide solution to be soaked 4 hours, then use the flowing water continuous flushing, constantly remove the pericarp residue that comes off in bag therebetween, until pericarp with after seed separates fully, further clean out the pericarp residue, namely obtain clean wasabi seed;
(3) seed disinfection: after the wasabi seed that obtains is blotted the moisture on surface with filter paper, put into 0.5% Polyoxin solution and soaked 3 hours;
(4) seed dries: wasabi seed after sterilizing with sterile water wash 2～5 times after, be placed under the environmental condition of 22 ℃ and dry in the shade, measure the variation of seed moisture content;
(5) the freezing preservation of seed: when the water content that records seed is 15%, in its nylon net bag of having sterilized of packing into, every packed 1/3 left and right seed, then put into the refrigerator-freezer that is built-in with the multilayer shelf, every bag of seed is tiled on shelf equably, and the refrigerator-freezer temperature remains on 5 ℃, and refrigerator-freezer bottom is placed with the saturated aqueous common salt of one glass of 250mL, 12 centimetres apart from refrigerator-freezer bottom of the bottom shelf of described multilayer shelf, every layer of shelf is at a distance of 8 centimetres.Wasabi seed can be preserved more than 1 year and be mildewproof, and the Seed germination rate is 65.4%.
Comparing embodiment 1
Embodiment 1 step (1) is changed into: the horseradish fruit of gathering is sub-packed in nylon net bag, is placed in 40 ℃, the environmental condition Xiayang of direct sunlight and did 5 days, other steps are with embodiment 1, and wasabi seed is after preserving 1 year, and germination rate only has 30.2%.
Comparing embodiment 2
Cancel embodiment 1 step (3), other steps are with embodiment 1, and preservation is after 4 months in refrigerator-freezer for wasabi seed, and mouldy phenomenon appears in the part seed, explanation thus, and seed disinfection before preservation is very important.
Comparing embodiment 3
Change the water content of seed in embodiment 1 step (5) into 45% by 25%, other steps are with embodiment 1, and wasabi seed has situation mouldy and that sprout in advance and produces in preserving process.
Comparing embodiment 4
In step (5), saturated aqueous common salt is not placed in the refrigerator-freezer bottom, and other steps are with embodiment 1, and the germination rate of wasabi seed after preserving 1 year is 73.1%.
Claims (4)
1. the method for preserving of a wasabi seed, is characterized in that comprising the steps:
(1) fruit is dry: the fruit of gathering is sub-packed in nylon net bag, is placed in and is not subjected to that direct sunlight, ventilation condition are good, temperature is that the environment of 18～25 ℃ dries in the shade, frequently stir therebetween;
(2) fast except pericarp: dry in the shade when easily separating with seed to pericarp until fruit, fruit is put into hydrogen peroxide solution soak, then rinse with flowing water, constantly remove the pericarp residue that comes off in bag therebetween, with after seed separates fully, remove the pericarp residue until pericarp, namely obtain clean wasabi seed;
(3) seed disinfection: after the wasabi seed that obtains is blotted the moisture on surface with filter paper, put into Polyoxin solution and soak;
(4) seed dries: the wasabi seed after sterilizing with sterile water wash after, be placed under the environmental condition of 15～25 ℃ and dry in the shade, measure the variation of seed moisture content;
(5) the freezing preservation of seed: when the water content that records seed is 15～35%, in its nylon net bag of having sterilized of packing into, put into again the refrigerator-freezer that is built-in with the multilayer shelf, every bag of seed is tiled on shelf equably, the refrigerator-freezer temperature remains on 3～8 ℃, be placed with saturated aqueous common salt in refrigerator-freezer, the bottom shelf of described multilayer shelf should be apart from the certain distance in refrigerator-freezer bottom.
2. the method for preserving of a kind of wasabi seed according to claim 1 is characterized in that: in step (2), hydrogen peroxide solution concentration is 1%～5%, and soak time is 4～6 hours.
3. the method for preserving of a kind of wasabi seed according to claim 1 is characterized in that: in step (3), the concentration of Polyoxin solution is 0.1%～0.5%, and soak time is 2～8 hours.
4. the method for preserving of a kind of wasabi seed according to claim 1 is characterized in that: in step (5) every layer of shelf at a distance of 5～10 centimetres, 10～15 centimetres apart from the refrigerator-freezer bottom of bottom shelf.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310059779.XA CN103081607B (en) | 2013-02-26 | 2013-02-26 | Preservation method of horseradish seeds |
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| CN201310059779.XA CN103081607B (en) | 2013-02-26 | 2013-02-26 | Preservation method of horseradish seeds |
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| CN103081607A true CN103081607A (en) | 2013-05-08 |
| CN103081607B CN103081607B (en) | 2014-12-03 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103829347A (en) * | 2014-03-05 | 2014-06-04 | 大姚家和天然食品开发有限责任公司 | Freshness retaining technology for Chinese prickly ash |
| CN111466285A (en) * | 2020-04-20 | 2020-07-31 | 成都大学 | Method for planting horseradish plants in water area environment |
| CN112514751A (en) * | 2020-11-30 | 2021-03-19 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Long-term storage and recovery method of cassava crossbreeding capsule |
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| WO2010090517A1 (en) * | 2009-02-03 | 2010-08-12 | Monsanto Holland B.V. | Enriching the seed quality of a batch of seeds |
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
-
2013
- 2013-02-26 CN CN201310059779.XA patent/CN103081607B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010090517A1 (en) * | 2009-02-03 | 2010-08-12 | Monsanto Holland B.V. | Enriching the seed quality of a batch of seeds |
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
Non-Patent Citations (4)
| Title |
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| 吴明根 等: "山葵生物生态特性的研究", 《延边大学农学学报》, vol. 22, no. 3, 30 September 2000 (2000-09-30), pages 211 - 215 * |
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| 莫慧机 等: "日本山葵的开花结实特性及留种育苗技术", 《广东农业科学》, no. 5, 25 October 2004 (2004-10-25), pages 23 - 25 * |
| 蔡光泽: "山葵种子的采集、处理及贮藏", 《种子》, no. 6, 30 December 2002 (2002-12-30), pages 106 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103829347A (en) * | 2014-03-05 | 2014-06-04 | 大姚家和天然食品开发有限责任公司 | Freshness retaining technology for Chinese prickly ash |
| CN103829347B (en) * | 2014-03-05 | 2015-11-25 | 大姚家和天然食品开发有限责任公司 | A kind of antistaling process of Chinese prickly ash |
| CN111466285A (en) * | 2020-04-20 | 2020-07-31 | 成都大学 | Method for planting horseradish plants in water area environment |
| CN111466285B (en) * | 2020-04-20 | 2022-01-11 | 成都大学 | Method for planting horseradish plants in water area environment |
| CN112514751A (en) * | 2020-11-30 | 2021-03-19 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Long-term storage and recovery method of cassava crossbreeding capsule |
| CN112514751B (en) * | 2020-11-30 | 2022-06-17 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Long-term storage and recovery method of cassava crossbreeding capsule |
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