CN110140472A - A method of promote paris polyphylla seed to sprout - Google Patents
A method of promote paris polyphylla seed to sprout Download PDFInfo
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- CN110140472A CN110140472A CN201910435343.3A CN201910435343A CN110140472A CN 110140472 A CN110140472 A CN 110140472A CN 201910435343 A CN201910435343 A CN 201910435343A CN 110140472 A CN110140472 A CN 110140472A
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- Prior art keywords
- paris polyphylla
- seed
- polyphylla seed
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- peg
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of methods that promotion paris polyphylla seed is sprouted, comprising the following steps: step 1: chooses the mature and plump seed of paris polyphylla natural cracking；Step 2: the paris polyphylla seed dried is dipped into 24-84h in the mixed liquor of gibberellin and PEG (polyethylene glycol), the concentration of gibberellin is 400-600 μM in the mixed liquor, the mass concentration of PEG is 15-40% in the mixed liquor, and soaking process is in the paris polyphylla seed under natural lighting；Step 3: the paris polyphylla seed after immersion is placed in sand, and watering carries out constant temperature dark culture.This method passes through GA compared with the reproduction technique of existing paris polyphylla3With the synergistic effect of PEG, its suspend mode can be broken completely, the germination rate of fresh seeds only needs 4 months up to 85% or more from seed treatment to sprouting, greatly shortens the period of seed sprouting, and seed germination rate height, robust seedling, neat, transplanting survival rate height.
Description
Technical field
The invention belongs to technical field of plant propagation, and in particular to a method of promote paris polyphylla seed to sprout.
Background technique
Paris polyphylla (Paris polyphylla var.chinensi) also known as paris polyphylla belong to Liliaceae Paris, mainly
The areas such as Hubei, Hunan, Jiangxi, Zhejiang, Yunnan, Guizhou, Sichuan are distributed in, the certified products medicine source as Paris polyphylla is by " the Chinese people
Republic's pharmacopeia " it includes, it is the important of a variety of Chinese patent drugs such as Yunnan Baiyao, tablet for curing menorrhagia, metrorrhagia and chronic ipelvic inflammation, analgesic anticarcinogen ball and antivirus powder
One of raw material, annual requirement are more than 10,000 tons.Medicinal paris polyphylla is to acquire based on wild resource, with the evil of ecological environment
Change under the dual-pressure for blindly pursuing economic interests with people, there is a serious shortage in the supply due to robbing the exploitation of formula for wild resource, far from
It is able to satisfy the market demand, and artificial propagation cultivation is the weight protected paris polyphylla resource and meet related industry sustainable development
Want approach.In the prior art, paris polyphylla artificial propagation is mainly to excavate based on wild resource transplanting, but its breeding coefficient
Low, dosage is big, at high cost, and the period is long (2~4 years), is difficult to realize large-scale production.And seminal propagation is used, in natural conditions
Under, there is " secondary dormancy " phenomenon in seed, the sprouting period is long, and germination rate is low, and it is irregular to emerge.And use low-temperature stratification,
The methods of alternating temperature stratification can accelerate the sprouting of seed to a certain extent, but also can not really realize paris polyphylla (Hua Chonglou)
The problems such as seed current year is handled, second year Germination And Seedling, and sprouting is irregular, and emergence rate is lower.
Summary of the invention
For paris polyphylla seed, in the presence of sprouting, the period is long, and the low problem of germination rate, the invention discloses a kind of promotions
The method that paris polyphylla seed is sprouted, this method have the advantages that easy to operate, fast and effective, can be realized and acquire seed the
2 years seedlings.
The invention discloses a kind of methods that promotion paris polyphylla seed is sprouted, comprising the following steps:
Step 1: the seed of paris polyphylla is chosen；
Step 2: paris polyphylla seed is dipped into 24-84h in the mixed liquor of gibberellin and PEG, in the mixed liquor
The concentration of gibberellin is 400-600 μM, and the mass concentration of PEG is 15-40% in the mixed liquor, and soaking process makes seven leaf
A flower seed is under natural lighting；
Step 3: the paris polyphylla seed after immersion is placed in sand, and watering carries out dark culture.
Further, in the step 1, the mature and plump seed of paris polyphylla natural cracking is chosen, color is dark red
Color.
Further, in the step 1, paris polyphylla seed is peelled off into glume, at room temperature natural air drying 2-3 days.
Further, in the step 1, the paris polyphylla seed of 4~-20 DEG C of cryo-conservations is chosen.
Further, in the step 2, PEG is selected from PEG4000-10000, concentration 25%.
Further, in the step 3 by the paris polyphylla seed after immersion with aseptic water washing 3-5 times.
Further, in the step 3, sand is through 121 DEG C, and sterilize 30min under 0.1KPa, naturally dry.
Further, in the step 3, the paris polyphylla seed after immersion is placed on sand, sand upper layer again
One layer of sand of lid, lower layer's sand with a thickness of 1.5-2cm, upper layer sand with a thickness of 0.5-1.0cm, watering.
Further, in the step 3, when carrying out dark culture, the cultivation temperature of constant incubator is 20 ± 2 DEG C.
Inventor has found that Gibberellins solution and PEG solution have the rudiment of paris polyphylla seed through a large number of experiments
There are collaboration facilitation, the especially mixed liquor of 400-600 μM of gibberellin and 15-40%PEG, in combination with natural lighting and secretly
Culture combines, and can break the suspend mode of paris polyphylla seed completely, the germination rate of fresh seeds is up to 85% or more, from kind
Subprocessing is sprouted to seed and only needs 4 months, and the period of seed sprouting is greatly shortened, and is sprouted using the seed that the method is handled
Hair rate height, robust seedling, neat, transplanting survival rate is high.
Specific embodiment
The invention discloses a kind of methods that promotion paris polyphylla seed is sprouted, and this method has easy to operate, quick
Effective advantage can be realized acquisition seed second year seedling.
The technical solution in the present invention will be clearly and completely described below, it is clear that described is only this hair
Bright a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not having
Every other embodiment obtained under the premise of creative work is made, shall fall within the protection scope of the present invention.
The invention discloses a kind of methods that promotion paris polyphylla seed is sprouted, comprising the following steps:
Step 1: the seed of paris polyphylla is chosen；
Step 2: paris polyphylla seed is dipped into 24-84h in the mixed liquor of gibberellin and PEG, in the mixed liquor
The concentration of gibberellin is 400-600 μM, and the mass concentration of PEG is 15-40% in the mixed liquor, and soaking process makes seven leaf
A flower seed is under natural lighting；
Step 3: the paris polyphylla seed after immersion is placed in sand, and watering carries out dark culture.
Inventor has found that Gibberellins solution and PEG solution have the rudiment of paris polyphylla seed through a large number of experiments
There are collaboration facilitation, the especially mixed liquor of 400-600 μM of gibberellin and 15-40%PEG, in combination with natural lighting and secretly
Culture combines, and can break the suspend mode of paris polyphylla seed completely, the germination rate of fresh seeds is up to 85% or more, from kind
Subprocessing is sprouted to seed and only needs 4 months, and the period of seed sprouting is greatly shortened, and is sprouted using the seed that the method is handled
Hair rate height, robust seedling, transplanting survival rate are high.
In the step 1, the mature and plump seed of paris polyphylla natural cracking is chosen, color is peony.
In the step 1, paris polyphylla seed is peelled off into glume, at room temperature natural air drying 2-3 days.
Peel off the immersion infiltration that glume is conducive to subsequent Gibberellins solution and PEG solution to paris polyphylla seed.
In some embodiments, in the step 1, the glume of paris polyphylla seed can not also be peelled off.
In some embodiments, in the step 1, the paris polyphylla seed of 4~-20 DEG C of cryo-conservations is chosen, it is described
The paris polyphylla seed of 4~-20 DEG C of cryo-conservations is the seed of harvest upper one year.
In the step 2, PEG is selected from PEG4000-10000, concentration 25%.In conjunction with 400-600 μM of gibberellin, warp
The paris polyphylla seed germination rate highest of the processing is crossed, sprouting seedling is sturdy, and color is bottle green.
By the paris polyphylla seed after immersion with aseptic water washing 3-5 times in the step 3, by paris polyphylla
The soak of the surface of the seed removes.
In the step 3, sand is through 121 DEG C, and sterilize 30min under 0.1KPa, naturally dry.
In the step 3, the paris polyphylla seed after immersion is placed on sand, in sand upper layer one layer of sand of lid again
Son, lower layer's sand with a thickness of 1.5-2cm, upper layer sand with a thickness of 0.5-1.0cm, watering just observes sprouting seedling after 90d
(cotyledon has been up to 1cm, radicle 2-3cm at this time).
Sand surface be not easy it is mouldy, be conducive to seed sprouting.
It is 20 ± 2 DEG C by the cultivation temperature of constant incubator when carrying out dark culture in the step 3.
Before not observing that sprouting seedling breaks through sand, after on seed upper layer, sand parches completely, by the sand in the box that germinates
It irrigates, after sprouting seedling and breaking through sand, to keep the sand in germination box wet.
Below by way of specific embodiment, the present invention will be described:
Embodiment 1:
Present embodiment discloses a kind of methods that promotion paris polyphylla seed is sprouted, comprising the following steps:
Step 1: the mature and plump seed of paris polyphylla natural cracking is chosen, peels off glume, at room temperature natural air drying
2-3 days；
Step 2: being dipped into 84h in the mixed liquor of gibberellin and PEG for paris polyphylla seed, red in the mixed liquor
The concentration of mycin is 400 μM, and the mass concentration of PEG is 25% in the mixed liquor, and soaking process makes the paris polyphylla kind
Son is under natural lighting；
Step 3: by the paris polyphylla seed after immersion with aseptic water washing 3-5 times, by the paris polyphylla after immersion
Seed is placed on sand, in sand upper layer one layer of sand of lid again, lower layer's sand with a thickness of 1.5-2cm, the thickness of upper layer sand
For 0.5-1.0cm, watering carries out dark culture, and the cultivation temperature of constant incubator is 20 ± 2 DEG C.
Embodiment 2-12:
Embodiment 2-12 discloses a kind of method that promotion paris polyphylla seed is sprouted, including as embodiment 1 is most of
Operating procedure, the difference is that:
Using gibberellin and PEG concentration shown in embodiment 2-12 in table 1 and processing time.
Comparative example 1-2:
Comparative example 1-2 discloses a kind of method that promotion paris polyphylla seed is sprouted, including such as the most of behaviour of embodiment 1
Make step, the difference is that:
Using gibberellin and PEG concentration shown in comparative example 1-2 in table 1 and processing time.
As a result it records
The germination rate of the processed paris polyphylla seed of embodiment 1-12 and comparative example 1-2 is reported in Table 1 below.
Table 1
As shown in Table 1, it is individually handled relative to using gibberellin or PEG, at method provided by the invention
The germination rate of the paris polyphylla seed of reason has biggish promotion.
The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be said that
Specific implementation of the invention is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, exist
Under the premise of not departing from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to of the invention
Protection scope.
Claims (9)
1. a kind of method for promoting paris polyphylla seed to sprout, which comprises the following steps:
Step 1: the seed of paris polyphylla is chosen；
Step 2: being dipped into 24-84h in the mixed liquor of gibberellin and PEG for paris polyphylla seed, red mould in the mixed liquor
The concentration of element is 400-600 μM, and the mass concentration of PEG is 15-40% in the mixed liquor, and soaking process makes seven Ye Yizhi
Flower seed is under natural lighting；
Step 3: the paris polyphylla seed after immersion is placed in sand, and watering carries out dark culture.
2. a kind of method for promoting paris polyphylla seed to sprout according to claim 1, which is characterized in that the step
In one, the mature and plump seed of paris polyphylla natural cracking is chosen, color is peony.
3. a kind of method for promoting paris polyphylla seed to sprout according to claim 1, which is characterized in that the step
In one, paris polyphylla seed is peelled off into glume, at room temperature natural air drying 2-3 days.
4. a kind of method for promoting paris polyphylla seed to sprout according to claim 1, which is characterized in that the step
In one, the paris polyphylla seed of 4~-20 DEG C of cryo-conservations is chosen.
5. a kind of method for promoting paris polyphylla seed to sprout according to claim 1, which is characterized in that the step
In two, PEG is selected from PEG4000-10000, concentration 25%.
6. a kind of method for promoting paris polyphylla seed to sprout according to claim 1, which is characterized in that the step
By the paris polyphylla seed after immersion with aseptic water washing 3-5 times in three.
7. a kind of method for promoting paris polyphylla seed to sprout according to claim 1, which is characterized in that the step
In three, sand is through 121 DEG C, and sterilize 30min under 0.1KPa, naturally dry.
8. a kind of method for promoting paris polyphylla seed to sprout according to claim 1, which is characterized in that the step
In three, the paris polyphylla seed after immersion is placed on sand, in sand upper layer one layer of sand of lid again, the thickness of lower layer's sand
For 1.5-2cm, upper layer sand with a thickness of 0.5-1.0cm, watering.
9. a kind of method for promoting paris polyphylla seed to sprout according to claim 1, which is characterized in that the step
In three, when carrying out dark culture, the cultivation temperature of constant incubator is 20 ± 2 DEG C.
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| CN201910435343.3A CN110140472B (en) | 2019-05-23 | 2019-05-23 | Method for promoting germination of paris polyphylla seeds |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114223471A (en) * | 2021-10-19 | 2022-03-25 | 重庆师范大学 | Rapid seedling method of paris polyphylla |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114223471A (en) * | 2021-10-19 | 2022-03-25 | 重庆师范大学 | Rapid seedling method of paris polyphylla |
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