CN106258388A - A kind of plantation technique of Rhizoma Gastrodiae - Google Patents
A kind of plantation technique of Rhizoma Gastrodiae Download PDFInfo
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- CN106258388A CN106258388A CN201610663695.0A CN201610663695A CN106258388A CN 106258388 A CN106258388 A CN 106258388A CN 201610663695 A CN201610663695 A CN 201610663695A CN 106258388 A CN106258388 A CN 106258388A
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- rhizoma gastrodiae
- wooden stick
- bacterial strain
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses the plantation technique of a kind of Rhizoma Gastrodiae, described sterilization treatment that wooden stick is carried out disinfection, the efficiency of inoculation strain can be improved, thus the plantation of beneficially Rhizoma Gastrodiae, described fertile soil and fallen leaves are built rear top and are piled unrestrained circle, be conducive to draining, carry out mixing bacterium before the sowing of described seeds of Gastrodia elata to process with detoxification bacterial strain, the sprouting of seeds of Gastrodia elata and growth can there be is more significant raising, the purification and rejuvenation of described detoxification bacterial strain, can promote effectively to carry out with what seeds of Gastrodia elata was dressed seed, the composition of described Nutrition Soil and proportioning, the stem that can make Rhizoma Gastrodiae slightly increases slightly, improve the resistance of Rhizoma Gastrodiae, the Rhizoma Gastrodiae that this kind of implantation methods technique plants out has the advantage that Biomass is big and productivity is high, market potential is huge, have a extensive future.
Description
Technical field
The invention belongs to agricultural production method field, it is more particularly related to the plantation technique of a kind of Rhizoma Gastrodiae.
Background technology
Rhizoma Gastrodiae, is one of China's rare Chinese medicine, main product in northeast, northwest, Yunnan, the ground such as Sichuan and Hubei, along with right
Deepening continuously of Rhizoma Gastrodiae research, new pharmacological action is the most revealed, and the demand of Rhizoma Gastrodiae is also continuously increased, Wild gastrodia
Yield can not meet the demand in market, and the artificial culture of Rhizoma Gastrodiae progressively instead of wild excavating, become Rhizoma Gastrodiae mainly for
The source of goods.Therefore, understand the characteristic of Rhizoma Gastrodiae, plantation feature, technological process and existing problems, to promote gastrodin cultivation scale,
Standardization, batch production and mechanization have directive function.
Summary of the invention
Problem to be solved by this invention is to provide the plantation technique of a kind of Rhizoma Gastrodiae.
To achieve these goals, the technical scheme that the present invention takes is:
The plantation technique of a kind of Rhizoma Gastrodiae, comprises the steps:
(1) base material prepares
Choose ripe Betula, then Betula is cut into diameter 3-9 cm, the wooden stick of long 40-60 cm, every 4-on each wooden stick
6cm cuts a fish scale mouth；
(2) inoculation strain
Then by wooden stick at sand ground layer, and connect Armillaria mellea kind at every layer, and build with fertile soil and fallen leaves；
(3) base material is placed
Then ground surface is flattened, sprinkle the thick fertile soil of 1-3 cm at ground surface, then sprinkle 1 layer of drenched blue or green thick stick leaf of immersion
Son, is then spaced the wooden stick of inoculation strain by 2-4 cm, then fills the space between wooden stick with fertile soil；
(4) sowing
Being leaned against by seeds of Gastrodia elata in the space of wooden stick both sides and sow, be often separated by 14-16cm and put one, a kind is respectively put at wooden stick two ends
Son；
(5) backfill
Then filling out Nutrition Soil, until exceeding wooden stick 2-4cm, then covering fertile soil 6-10cm 1 layer, the top of wooden stick,
Cover 1 layer of leaves again；
(6) daily management
Carry out after Rhizoma Gastrodiae topdressing and aerosol apparatus water process, described in the number of times that topdresses be 1-3 time；
(7) gather
After transplanting 1-2 plucking Rhizoma Gastrodiae, pluck after being carried out, dry natural drying, packaging is dispatched from the factory.
Preferably, carry out disinfection in described step (1) sterilization treatment to wooden stick.
Preferably, in described step (2), fertile soil and fallen leaves are built rear top and are piled unrestrained circle.
Preferably, described step (4) carries out mix bacterium before seeds of Gastrodia elata sowing to process with detoxification bacterial strain.
Preferably, the purification and rejuvenation step of described detoxification bacterial strain is: 1. activation being treated, detoxification Germination Strain inoculation is at dress
Have in the plate of PDA culture medium, 25 DEG C of constant temperature culture, after colony diameter length to 3-5cm, cut bacterium colony edge children tender
Mycelia tip 0.2-0.5mm transfers in another plate, puts into 25 DEG C of constant temperature culture, when colony diameter is plate 2/3
For first generation bacterial strain；2. by first generation inoculation in the plate equipped with PDA culture medium, 25 DEG C of constant temperature culture, treat that bacterium colony is straight
Path length, to after 3-5cm, cuts the bacterium colony edge tender mycelia tip 0.2-0.5mm of children and transfers in another plate, put into 25
DEG C constant temperature culture, is the second filial generation bacterial strain when colony diameter is plate 2/3,3. by above-mentioned identical method, carries out successively
3-8 time, obtain detoxification bacterial strain.
Preferably, what described step (5) Middle nutrition was native consists of rural area soil, river sand, perlite and Pericarppium arachidis hypogaeae, described nutrition
The composition and ratio of soil is 0.5:0.5:0.5:0.5-1:1:1:1.
Beneficial effect: the invention provides the plantation technique of a kind of Rhizoma Gastrodiae, described sterilization treatment that wooden stick is carried out disinfection, can
To improve the efficiency of inoculation strain, thus the plantation of beneficially Rhizoma Gastrodiae, described fertile soil and fallen leaves are built rear top and are piled unrestrained circle
Shape, beneficially draining, carries out mixing bacterium with detoxification bacterial strain before the sowing of described seeds of Gastrodia elata and processes, can to the sprouting of seeds of Gastrodia elata and
Growth has more significant raising, the purification and rejuvenation of described detoxification bacterial strain, can promote effectively to carry out with what seeds of Gastrodia elata was dressed seed, institute
State composition and the proportioning of Nutrition Soil, the stem of Rhizoma Gastrodiae can be made slightly to increase slightly, improve the resistance of Rhizoma Gastrodiae, this kind of implantation methods technique
Plantation Rhizoma Gastrodiae out has the advantage that Biomass is big and productivity is high, and market potential is huge, has a extensive future.
Detailed description of the invention
Embodiment 1:
The plantation technique of a kind of Rhizoma Gastrodiae, comprises the steps:
(1) base material prepares
Choose ripe Betula, then Betula is cut into diameter 3cm, the wooden stick of long 40cm, each wooden stick cuts a fish every 4cm
Squama mouth, then carry out disinfection sterilization treatment to wooden stick；
(2) inoculation strain
Then by wooden stick at sand ground layer, and connect Armillaria mellea kind at every layer, and build with fertile soil and fallen leaves, institute
State fertile soil and fallen leaves are built rear top and piled unrestrained circle；
(3) base material is placed
Then ground surface is flattened, sprinkles the thick fertile soil of 1 cm at ground surface, then sprinkle 1 layer of drenched blue or green thick stick leaf of immersion,
Then the wooden stick of inoculation strain is spaced by 2 cm, then fills the space between wooden stick with fertile soil；
(4) sowing
Lean against after will carrying out mixing bacterium process with detoxification bacterial strain before seeds of Gastrodia elata sowing in the space of wooden stick both sides and sow, be often separated by
14cm puts one, and a seed is respectively put at wooden stick two ends, and the purification and rejuvenation step of described detoxification bacterial strain is: 1. activation being treated, detoxification is sprouted
Send out bacteria strain to be seeded in the plate equipped with PDA culture medium, 25 DEG C of constant temperature culture, after colony diameter length to 3cm, cut
The bacterium colony edge tender mycelia tip 0.2mm of children transfers in another plate, puts into 25 DEG C of constant temperature culture, and colony diameter is flat
It it is the first generation bacterial strain during ware 2/3；2. by first generation inoculation in the plate equipped with PDA culture medium, 25 DEG C of constant temperature trainings
Support, after colony diameter length to 3-5cm, cut the bacterium colony edge tender mycelia tip 0.2mm of children and transfer in another plate,
Put into 25 DEG C of constant temperature culture, be the second filial generation bacterial strain when colony diameter is plate 2/3,3. by above-mentioned identical method, successively
Carry out the 3rd time, obtain detoxification bacterial strain；
(5) backfill
Then fill out Nutrition Soil, until exceeding wooden stick 2cm, then covering fertile soil 6cm 1 layer, the top of wooden stick, then covering
1 layer of leaves, described Nutrition Soil consist of rural area soil, river sand, perlite and Pericarppium arachidis hypogaeae, the composition and ratio of described Nutrition Soil is
0.5:0.5:0.5: 0.5；
(6) daily management
Carry out after Rhizoma Gastrodiae topdressing and aerosol apparatus water process, described in the number of times that topdresses be 1 time；
(7) gather
After transplanting 1 year plucking Rhizoma Gastrodiae, pluck after being carried out, dry natural drying, packaging is dispatched from the factory.
Embodiment 2:
The plantation technique of a kind of Rhizoma Gastrodiae, comprises the steps:
(1) base material prepares
Choose ripe Betula, then Betula is cut into diameter 6 cm, the wooden stick of long 50 cm, each wooden stick cuts one every 5cm
Fish scale mouth, then carry out disinfection sterilization treatment to wooden stick；
(2) inoculation strain
Then by wooden stick at sand ground layer, and connect Armillaria mellea kind at every layer, and build with fertile soil and fallen leaves, institute
State fertile soil and fallen leaves are built rear top and piled unrestrained circle；
(3) base material is placed
Then ground surface is flattened, sprinkles the thick fertile soil of 2 cm at ground surface, then sprinkle 1 layer of drenched blue or green thick stick leaf of immersion,
Then the wooden stick of inoculation strain is spaced by 3 cm, then fills the space between wooden stick with fertile soil；
(4) sowing
Lean against after will carrying out mixing bacterium process with detoxification bacterial strain before seeds of Gastrodia elata sowing in the space of wooden stick both sides and sow, be often separated by
15cm puts one, and a seed is respectively put at wooden stick two ends, and the purification and rejuvenation step of described detoxification bacterial strain is: 1. activation being treated, detoxification is sprouted
Send out bacteria strain to be seeded in the plate equipped with PDA culture medium, 25 DEG C of constant temperature culture, after colony diameter length to 4cm, cut
The bacterium colony edge tender mycelia tip 0.35mm of children transfers in another plate, puts into 25 DEG C of constant temperature culture, and colony diameter is
It it is the first generation bacterial strain during plate 2/3；2. by first generation inoculation in the plate equipped with PDA culture medium, 25 DEG C of constant temperature trainings
Support, after colony diameter length to 4cm, cut the bacterium colony edge tender mycelia tip 0.35mm of children and transfer in another plate,
Put into 25 DEG C of constant temperature culture, be the second filial generation bacterial strain when colony diameter is plate 2/3,3. by above-mentioned identical method, successively
Carry out the 5th time, obtain detoxification bacterial strain；
(5) backfill
Then fill out Nutrition Soil, until exceeding wooden stick 3cm, then covering fertile soil 8cm 1 layer, the top of wooden stick, then covering
1 layer of leaves, described Nutrition Soil consist of rural area soil, river sand, perlite and Pericarppium arachidis hypogaeae, the composition and ratio of described Nutrition Soil is
0.75:0.75:0.75: 0.75；
(6) daily management
Carry out after Rhizoma Gastrodiae topdressing and aerosol apparatus water process, described in the number of times that topdresses be 2 times；
(7) gather
After transplanting 1.5 years plucking Rhizoma Gastrodiae, pluck after being carried out, dry natural drying, packaging is dispatched from the factory.
Embodiment 3
The plantation technique of a kind of Rhizoma Gastrodiae, comprises the steps:
(1) base material prepares
Choose ripe Betula, then Betula is cut into diameter 9 cm, the wooden stick of long 60 cm, each wooden stick cuts one every 6cm
Fish scale mouth, then carry out disinfection sterilization treatment to wooden stick；
(2) inoculation strain
Then by wooden stick at sand ground layer, and connect Armillaria mellea kind at every layer, and build with fertile soil and fallen leaves, institute
State fertile soil and fallen leaves are built rear top and piled unrestrained circle；
(3) base material is placed
Then ground surface is flattened, sprinkles the thick fertile soil of 3 cm at ground surface, then sprinkle 1 layer of drenched blue or green thick stick leaf of immersion,
Then the wooden stick of inoculation strain is spaced by 4 cm, then fills the space between wooden stick with fertile soil；
(4) sowing
Lean against after will carrying out mixing bacterium process with detoxification bacterial strain before seeds of Gastrodia elata sowing in the space of wooden stick both sides and sow, be often separated by
16cm puts one, and a seed is respectively put at wooden stick two ends, and the purification and rejuvenation step of described detoxification bacterial strain is: 1. activation being treated, detoxification is sprouted
Send out bacteria strain to be seeded in the plate equipped with PDA culture medium, 25 DEG C of constant temperature culture, after colony diameter length to 5cm, cut
The bacterium colony edge tender mycelia tip 0.5mm of children transfers in another plate, puts into 25 DEG C of constant temperature culture, and colony diameter is flat
It it is the first generation bacterial strain during ware 2/3；2. by first generation inoculation in the plate equipped with PDA culture medium, 25 DEG C of constant temperature trainings
Support, after colony diameter length to 5cm, cut the bacterium colony edge tender mycelia tip 0.5mm of children and transfer in another plate, put
Enter 25 DEG C of constant temperature culture, be the second filial generation bacterial strain when colony diameter is plate 2/3,3. by above-mentioned identical method, enter successively
Row the 8th time, obtains detoxification bacterial strain；
(5) backfill
Then fill out Nutrition Soil, until exceeding wooden stick 4cm, then covering fertile soil 10cm 1 layer, the top of wooden stick, then covering
Upper 1 layer of leaves, described Nutrition Soil consist of rural area soil, river sand, perlite and Pericarppium arachidis hypogaeae, the composition and ratio of described Nutrition Soil is
1:1:1:1；
(6) daily management
Carry out after Rhizoma Gastrodiae topdressing and aerosol apparatus water process, described in the number of times that topdresses be 3 times；
(7) gather
After transplanting 2 years plucking Rhizoma Gastrodiae, pluck after being carried out, dry natural drying, packaging is dispatched from the factory.
After above method, taking out sample respectively, measurement result is as follows:
| Detection project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Existing index |
| Percentage of seedgermination (%) | 98 | 99 | 97 | 90 |
| Survival rate (%) | 95 | 99 | 94 | 91 |
| Average biomass (g) | 214.8 | 221.1 | 209.3 | 188.5 |
Can draw according to above table data, when embodiment 2 parameter, the Rhizoma Gastrodiae after plantation is than the sky after prior art plantation
The percentage of seedgermination of fiber crops is high, and survival rate and average biomass increase, and are now more beneficial for the plantation of Rhizoma Gastrodiae.
The invention provides the plantation technique of a kind of Rhizoma Gastrodiae, described sterilization treatment that wooden stick is carried out disinfection, can improve and connect
The efficiency of kind of strain, thus the plantation of beneficially Rhizoma Gastrodiae, described fertile soil and fallen leaves are built rear top and are piled unrestrained circle, are conducive to
Draining, carries out mixing bacterium and processes, can have the sprouting of seeds of Gastrodia elata and growth relatively with detoxification bacterial strain before the sowing of described seeds of Gastrodia elata
Significantly improve, the purification and rejuvenation of described detoxification bacterial strain, can promote effectively to carry out with what seeds of Gastrodia elata was dressed seed, described Nutrition Soil
Composition and proportioning, the stem of Rhizoma Gastrodiae can be made slightly to increase slightly, improve Rhizoma Gastrodiae resistance, this kind of implantation methods technique plants out
Rhizoma Gastrodiae there is the advantage that Biomass is big and productivity is high, market potential is huge, has a extensive future.
The foregoing is only embodiments of the invention, not thereby limit the scope of the claims of the present invention, every utilize this
Equivalent structure or equivalence flow process that bright description is made convert, or are directly or indirectly used in other relevant technology necks
Territory, is the most in like manner included in the scope of patent protection of the present invention.
Claims (6)
1. the plantation technique of a Rhizoma Gastrodiae, it is characterised in that comprise the steps:
(1) base material prepares
Choose ripe Betula, then Betula is cut into diameter 3-9 cm, the wooden stick of long 40-60 cm, every 4-on each wooden stick
6cm cuts a fish scale mouth；
(2) inoculation strain
Then by wooden stick at sand ground layer, and connect Armillaria mellea kind at every layer, and build with fertile soil and fallen leaves；
(3) base material is placed
Then ground surface is flattened, sprinkle the thick fertile soil of 1-3 cm at ground surface, then sprinkle 1 layer of drenched blue or green thick stick leaf of immersion
Son, is then spaced the wooden stick of inoculation strain by 2-4 cm, then fills the space between wooden stick with fertile soil；
(4) sowing
Being leaned against by seeds of Gastrodia elata in the space of wooden stick both sides and sow, be often separated by 14-16cm and put one, a kind is respectively put at wooden stick two ends
Son；
(5) backfill
Then filling out Nutrition Soil, until exceeding wooden stick 2-4cm, then covering fertile soil 6-10cm 1 layer, the top of wooden stick,
Cover 1 layer of leaves again；
(6) daily management
Carry out after Rhizoma Gastrodiae topdressing and aerosol apparatus water process, described in the number of times that topdresses be 1-3 time；
(7) gather
After transplanting 1-2 plucking Rhizoma Gastrodiae, pluck after being carried out, dry natural drying, packaging is dispatched from the factory.
2. according to the plantation technique of a kind of Rhizoma Gastrodiae described in claim 1, it is characterised in that: wooden stick is entered in (1) by described step
Row sterilization processes.
3. according to the plantation technique of a kind of Rhizoma Gastrodiae described in claim 1, it is characterised in that: in described step (2) fertile soil and
Fallen leaves are built rear top and are piled unrestrained circle.
4. according to the plantation technique of a kind of Rhizoma Gastrodiae described in claim 1, it is characterised in that: seeds of Gastrodia elata in described step (4)
Carry out mixing bacterium before sowing to process with detoxification bacterial strain.
5. according to the plantation technique of a kind of Rhizoma Gastrodiae described in claim 4, it is characterised in that: the purification and rejuvenation of described detoxification bacterial strain
Step is: 1. activation is treated detoxification Germination Strain inoculation in the plate equipped with PDA culture medium, 25 DEG C of constant temperature culture, treat bacterium
The diameter length that falls, to after 3-5cm, cuts the bacterium colony edge tender mycelia tip 0.2-0.5mm of children and transfers in another plate, put
Enter 25 DEG C of constant temperature culture, be the first generation bacterial strain when colony diameter is plate 2/3；2. by first generation inoculation equipped with
In the plate of PDA culture medium, 25 DEG C of constant temperature culture, after colony diameter length to 3-5cm, cut the bacterium colony edge tender bacterium of children
The most advanced and sophisticated 0.2-0.5mm of silk transfers in another plate, puts into 25 DEG C of constant temperature culture, when colony diameter is plate 2/3 is
Second filial generation bacterial strain, 3. by above-mentioned identical method, carries out the 3-8 time successively, obtains detoxification bacterial strain.
6. according to the plantation technique of a kind of Rhizoma Gastrodiae described in claim 1, it is characterised in that: described step (5) Middle nutrition soil
Consisting of rural area soil, river sand, perlite and Pericarppium arachidis hypogaeae, the composition and ratio of described Nutrition Soil is 0.5:0.5:0.5:0.5-1:1:
1:1。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610663695.0A CN106258388A (en) | 2016-08-15 | 2016-08-15 | A kind of plantation technique of Rhizoma Gastrodiae |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610663695.0A CN106258388A (en) | 2016-08-15 | 2016-08-15 | A kind of plantation technique of Rhizoma Gastrodiae |
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| CN106258388A true CN106258388A (en) | 2017-01-04 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107197668A (en) * | 2017-05-27 | 2017-09-26 | 贵州省德均农特产品开发有限公司 | Rhizoma Gastrodiae implantation methods |
| CN108812280A (en) * | 2018-06-28 | 2018-11-16 | 贵州省德均农特产品开发有限公司 | A kind of household Rhizoma Gastrodiae planting unit and the method for planting rhizoma gastrodiae |
| CN116694481A (en) * | 2023-06-06 | 2023-09-05 | 陕西理工大学 | Detoxification screening method for gastrodia elata germination bacteria |
-
2016
- 2016-08-15 CN CN201610663695.0A patent/CN106258388A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107197668A (en) * | 2017-05-27 | 2017-09-26 | 贵州省德均农特产品开发有限公司 | Rhizoma Gastrodiae implantation methods |
| CN107197668B (en) * | 2017-05-27 | 2020-04-28 | 贵州省德均农特产品开发有限公司 | Gastrodia elata planting method |
| CN108812280A (en) * | 2018-06-28 | 2018-11-16 | 贵州省德均农特产品开发有限公司 | A kind of household Rhizoma Gastrodiae planting unit and the method for planting rhizoma gastrodiae |
| CN116694481A (en) * | 2023-06-06 | 2023-09-05 | 陕西理工大学 | Detoxification screening method for gastrodia elata germination bacteria |
| CN116694481B (en) * | 2023-06-06 | 2024-04-16 | 陕西森盛菌业科技有限公司 | Detoxification screening method for gastrodia elata germination bacteria |
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