CN109169016A - A kind of mating system that paris polyphylla seed quickly comes into leaves - Google Patents
A kind of mating system that paris polyphylla seed quickly comes into leaves Download PDFInfo
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- CN109169016A CN109169016A CN201811116626.3A CN201811116626A CN109169016A CN 109169016 A CN109169016 A CN 109169016A CN 201811116626 A CN201811116626 A CN 201811116626A CN 109169016 A CN109169016 A CN 109169016A
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- 241000244987 Daiswa polyphylla Species 0.000 title claims abstract description 109
- 230000013011 mating Effects 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000003375 plant hormone Substances 0.000 claims abstract description 9
- 230000000694 effects Effects 0.000 claims abstract description 7
- 239000004576 sand Substances 0.000 claims description 36
- 239000002689 soil Substances 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 229930191978 Gibberellin Natural products 0.000 claims description 20
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 20
- 239000003448 gibberellin Substances 0.000 claims description 20
- 230000001737 promoting effect Effects 0.000 claims description 13
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 12
- 235000011613 Pinus brutia Nutrition 0.000 claims description 12
- 241000018646 Pinus brutia Species 0.000 claims description 12
- 239000002775 capsule Substances 0.000 claims description 12
- 238000005336 cracking Methods 0.000 claims description 12
- 239000012535 impurity Substances 0.000 claims description 12
- 239000011159 matrix material Substances 0.000 claims description 12
- 230000003020 moisturizing effect Effects 0.000 claims description 12
- 238000010899 nucleation Methods 0.000 claims description 12
- 239000005416 organic matter Substances 0.000 claims description 12
- 230000035699 permeability Effects 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 238000002834 transmittance Methods 0.000 claims description 12
- 230000005540 biological transmission Effects 0.000 claims description 11
- 238000005286 illumination Methods 0.000 claims description 4
- 230000000638 stimulation Effects 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 6
- 241000411851 herbal medicine Species 0.000 abstract description 2
- 230000001850 reproductive effect Effects 0.000 abstract description 2
- 238000009790 rate-determining step (RDS) Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 15
- 239000003337 fertilizer Substances 0.000 description 11
- 239000000758 substrate Substances 0.000 description 11
- 238000009331 sowing Methods 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 238000009412 basement excavation Methods 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000005059 dormancy Effects 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 230000014284 seed dormancy process Effects 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- VNFPBHJOKIVQEB-UHFFFAOYSA-N clotrimazole Chemical compound ClC1=CC=CC=C1C(N1C=NC=C1)(C=1C=CC=CC=1)C1=CC=CC=C1 VNFPBHJOKIVQEB-UHFFFAOYSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention provides a kind of mating systems that paris polyphylla seed quickly comes into leaves, and belong to Chinese herbal medicine planting technology field, and the mating system is to promote seed to sprout by the collective effect for the plant hormone that relents；Then by the variation of temperature from low to high, the seed sprouted is promoted to come into leaves.Including low-temperature treatment seed, shell is peelled off, removes red skin, promotees bud processing, seed program request, growth promotion rate-determining steps.The seedling-growing time that paris polyphylla seed is substantially reduced using method of the invention improves the reproductive efficiency of paris polyphylla, is conducive to the large-scale plantation of paris polyphylla.
Description
Technical field
The invention belongs to Chinese herbal medicine cultivation technology fields, and in particular to a kind of breeding that paris polyphylla seed quickly comes into leaves
Method.
Background technique
Paris polyphylla ParispolyphyllaSmithvar.chinensis (Franch.) Hara is liliaceous plant
Parts of generic medicinal plants is recorded together with paris polyphylla in version over the years " Chinese Pharmacopoeia ", is one of Paris polyphylla certified products medicine source.It is that " Yunnan is white
One of primary raw material of famous Chinese patent drug such as medicine ", " Pien Tze Huang ", " jidesheng sheyao tablets ", " Gong Xue Ning ", " heat toxin is clear ", year need
16000 tons of the amount of asking or so, herb resource all rob formula excavation year by year, cause sternly to wild resource from wild by excavation at present
The destruction of weight, resource has been on the verge of in imminent danger at present.Carry out the research of artificial sapling multiplication technology, develop artificial growth, provides effectively
Resource be protection the most effective measure of wild resource.
Paris polyphylla growth and development is very slow, and harvesting needs 8-10 from seed to medicinal material, and seed dormancy phase is long, nursery
Technology is difficult.Seed needs to be unearthed by 2 winters under field conditions (factors), and emergence rate is very low.One, seven leaf in recent years
It spends equal Paris polyphylla to develop area to expand rapidly, source of seedling based on wild excavation transplanting, do not break through also by artificial breeding technique, though
There are many documents, patent to have an open paris polyphylla fast breeding technology, but germination rate often to sprout, long root as index, seven leaves
Conventional that seedling can be also just come into leaves into after 1 year after the long root of a flower seed, therefore, paris polyphylla seed, which is unearthed, to come into leaves
It just can guarantee emergence rate afterwards, it is ensured that paris polyphylla has provenance to carry out large-scale plantation.
Summary of the invention
For this reason, it may be necessary to a kind of method that paris polyphylla seed quickly comes into leaves be provided, to improve the plantation of paris polyphylla
Efficiency.Solve the problems such as paris polyphylla seed seedling-raising time is long, emergence rate is low.
To achieve the above object, following technical solution is inventor provided:
A kind of mating system that paris polyphylla seed quickly comes into leaves, the mating system are swashed by the plant that relents
The collective effect of element promotes seed to sprout；Then by the variation of temperature from low to high, the seed sprouted is promoted to come into leaves.
The mating system is the following steps are included: low-temperature treatment paris polyphylla seed；Shell is peelled off, red skin is removed；
Moisture-heat preservation promotees bud and handles seed；It is sprouted with plant hormone stimulation seed；Seed is put be sowed at nursery Tanaka at low temperature；Control
Illumination waits emerging.
Wherein, the plant hormone includes gibberellin.
Further, specific step is as follows for the mating system:
The first step selects pericarp cracking, pericarp turns yellow, mature paris polyphylla seed and is put into 2-8 DEG C, low-temperature treatment
10-15 days；Low-temperature treatment seed breaks seed first time athermobiosis.
The paris polyphylla seed capsules for completing low-temperature treatment are peelled off shell by second step, and fine sand and seed are pressed 1-3:1
Red skin is removed in mixing, stranding；Remove kind of a skin, removes kind of a skin mortifier, be conducive to emerge.
Third step, fine sand reduce seed infection and rotting rate with 4-8 hour of 100-120 DEG C of high-temperature sterilization, by fine sand and
The seed 4-6:1 for removing kind of skin is mixed thoroughly, moisturizing, water content 40-60%, sets 18-22 DEG C, is promoted bud and is handled 40-50 days；
4th step is mixed seed after promoting bud with 400-800mg/L gibberellin, is slightly dried；Seed after promoting bud is mixed with gibberellin, is beaten
Broken second of suspend mode of seed, is tested by the variation of Seed Germination enzyme, adds gibberellin to promote starch in the Seed sprouting later period
It is more preferable to promote bud effect for the hydrolysis of enzyme.
5th step turns over whole nursery field, selects abundant, loose, draining good permeability the sandy loam of the content of organic matter, soil pH
Value is advisable with 5.2-6.8.30cm or more is ploughed deeply, impurity in furrow, careful site preparation, the wide 1.0m-1.2m of furrow, the high 20cm- of furrow are rejected
25cm, furrow face are slightly tilted, and avoid ponding.Paris polyphylla seedling dedicated substrate 1000kg is applied per acre as base fertilizer, in furrow face
One layer of matrix uniformly is sprinkled, then is flattened plot with spike-tooth harrow, wait plant.
6th step is burrowed in whole good nursery field with the whole plate punch of deep 1-3cm, seeding row spacing 3cm х 3cm, will
Treated that the program request under natural low temperature of paris polyphylla seed is covered in accomplishing fluently cave with fertile soil for 4th step, not reveal
Seed is advisable, and is no more than 1-3cm on seed face, then cover one layer of 1-2cm thickness pine needle, irrigates root water.Wherein, it is described from
Right low temperature refers to environment temperature between 0 DEG C -5 DEG C.In order to guarantee that First Year can emerge, it should be sowing in January before the beginning of spring, allow kind
Son tides over natural low temperature in nursery, breaks dormancy of embryonic root.If too late sowing, do not pass through natural low temperature, then First Year
It will not emerge.
7th step, column draw sunshade net, carry out light transmission and sunshade work, keep light transmittance 30-50%, seed is allowed to educate
0 DEG C of -22 DEG C of natural temperatures variation is tided in seedling ground, with the raising of ground temperature, paris polyphylla seed starts to come into leaves after 1-2 months
Emergence.Paris polyphylla seed passes through illumination and moisture, and kind skin easily softens, and also with paris polyphylla habit, likes certain dissipate
Illumination is related, is easier to prominent emergence of coming into leaves of breaking in the seed coat.
The beneficial effects of the present invention are:
(1) low temperature and Plant hormone treatment seed are used, seed dormancy phase is broken, improves and promotees bud effect.Using from low temperature
It is gradually warmed up, breaks dormancy of embryonic root, be more advantageous to seed cotyledons growth, emergence.
(2) paris polyphylla seed is sowed before the beginning of spring, First Year emergence can be effectively ensured, and highest emergence rate can
Up to 80%, is conducive to paris polyphylla and carries out large-scale plantation.
(3) seedling-growing time that paris polyphylla seed is substantially reduced using method of the invention, improves one, seven leaf
Colored reproductive efficiency.
Detailed description of the invention
Fig. 1 is the growing state that paris polyphylla seed difference described in specific embodiment sprouts the stage, in figure, from a left side
To the right sprouting stage is followed successively by original seed, an appearance of sprouting, sprout prominent, exposing radicle, fibrous root occur, root system development is complete
It sprouts at, cotyledon, plant that transdermally is bright, cotyledon is prominent breaks in the seed coat；
Fig. 2 is that paris polyphylla described in specific embodiment sows 3 months field emergence situations；
Fig. 3 is growing way after paris polyphylla described in specific embodiment emerges 4 months.
Specific embodiment
For technology contents, the objects and the effects that technical solution is described in detail, below in conjunction with specific embodiment and match
Attached drawing is closed to be explained in detail.
Embodiment 1
The first step, pericarp cracking is selected October, pericarp turns yellow, mature paris polyphylla seed and is put into 5-8 DEG C, low
Temperature processing 10-15 days；
Second step will complete low-temperature treatment paris polyphylla seed capsules and peel off shell, fine sand and seed are mixed by 2:1
It closes, red skin is removed in stranding, sees original seed of the first stage of Fig. 1；
Third step, fine sand were mixed thoroughly, moisturizing by fine sand with the seed 5:1 for removing kind of skin with 6 hours of 105 DEG C of high-temperature sterilizations,
Water content is about 50%, sets 22 DEG C, promotees bud and handles 40-50 days, sees the seed outstanding of sprouting of the phase III of Fig. 1；
4th step is mixed seed after promoting bud with 600mg/L gibberellin, is slightly dried；Seed after promoting bud is mixed with gibberellin, breaks kind
Sub second of suspend mode, is tested by the variation of Seed Germination enzyme, adds gibberellin to promote amylase in the Seed sprouting later period
It is more preferable to promote bud effect for hydrolysis.
5th step turns over whole nursery field, selects abundant, loose, draining good permeability the sandy loam of the content of organic matter, soil pH
Value is advisable with 5.2-6.8.It ploughs deeply 30cm or more, rejects impurity in furrow, careful site preparation, the wide 1.0m-1.2m of furrow, the high 20-25cm of furrow,
Furrow face is slightly tilted, and avoids ponding.Paris polyphylla seedling dedicated substrate 1000kg is applied per acre as base fertilizer, is uniformly spread in furrow face
Upper one layer of matrix, then flattened plot with spike-tooth harrow, wait plant.
6th step is burrowed, with the whole plate punch of deep 2cm, seeding row spacing 3cm х 3cm January in whole good nursery field
Part by the 4th step, treated that paris polyphylla seed seed program request is being accomplished fluently in cave, is then covered with fertile soil, not reveal
Seed is advisable, and it is thick to be no more than seed face 1cm, then cover one layer of 1-2cm thickness pine needle, irrigates root water.
7th step, column draw sunshade net, keep light transmittance 40%, mid-February, paris polyphylla seed started to come into leaves out
Seedling, from seed collection to come into leaves emerge when it is 4 months a length of.
The entire germination process of paris polyphylla seed is as shown in Figure 1, paris polyphylla sows 3 months field emergence situations such as
Shown in Fig. 2, growing way is as shown in Figure 3 after paris polyphylla emerges 4 months.Experimental result is shown, until late March paris polyphylla kind
The emergence rate of son is 90%.Wherein, emergence rate refer to paris polyphylla seed send out roots, leaf and unearthed number and total number seeds
Percentage.
Constantly changed by temperature and gibberellin Co stituation paris polyphylla seed is sprouted, and gibberellin must be in low temperature
It handles seed and room temperature promotees to be added after bud processing, i.e., be added in the Seed sprouting later period, gibberellin be added too early, seed is sprouted
There is no facilitation, seed mildew rate can be increased instead.
Embodiment 2
The first step, pericarp cracking is selected October, pericarp turns yellow, mature paris polyphylla seed and is put into 2-4 DEG C, low
Temperature processing 10-15 days；
Second step will complete low-temperature treatment paris polyphylla seed capsules and peel off shell, fine sand and seed are mixed by 2:1
It closes, red skin is removed in stranding；
Third step, fine sand were mixed thoroughly, moisturizing by fine sand with the seed 5:1 for removing kind of skin with 6 hours of 105 DEG C of high-temperature sterilizations,
Water content is about 50%, sets 22 DEG C, promotees bud and handles 40-50 days；
4th step is mixed seed after promoting bud with 600mg/L gibberellin, is slightly dried；
5th step turns over whole nursery field, selects abundant, loose, draining good permeability the sandy loam of the content of organic matter, soil pH
Value is advisable with 5.2-6.8.40cm is ploughed deeply, rejects impurity in furrow, careful site preparation, the wide 1.0m-1.2m of furrow, the high 25cm of furrow, furrow face is slightly
Low dip avoids ponding.Paris polyphylla seedling dedicated substrate 1000kg is applied per acre as base fertilizer, uniformly sprinkles one layer in furrow face
Matrix, then flattened plot with spike-tooth harrow, wait plant.
6th step is burrowed, with the whole plate punch of deep 2cm, seeding row spacing 3cm х 3cm January in whole good nursery field
Part by the 4th step, treated that the program request of paris polyphylla seed is being accomplished fluently in cave, is then covered with fertile soil, not reveal seed
It is advisable, it is thick is no more than seed face 1cm, then cover one layer of 1-2cm thickness pine needle, irrigates root water.
7th step carries out light transmission and sunshade work, keeps light transmittance 40%, mid-February, paris polyphylla seed started to grow
Leaf emergence, from seed collection to come into leaves emerge when it is 4 months a length of.
Experimental result is shown, until the emergence rate of late March paris polyphylla seed is 76%.
Embodiment 3
The first step, pericarp cracking is selected October, pericarp turns yellow, mature paris polyphylla seed and is put into 5-8 DEG C, low
Temperature processing 20-25 days；
Second step will complete low-temperature treatment paris polyphylla seed capsules and peel off shell, fine sand and seed are mixed by 2:1
It closes, red skin is removed in stranding；
Third step, fine sand were mixed thoroughly, moisturizing by fine sand with the seed 5:1 for removing kind of skin with 6 hours of 105 DEG C of high-temperature sterilizations,
Water content is about 50%, sets 22 DEG C, promotees bud and handles 30-40 days；
4th step is mixed seed after promoting bud with 600mg/L gibberellin, is slightly dried；
5th step turns over whole nursery field, selects abundant, loose, draining good permeability the sandy loam of the content of organic matter, soil pH
Value is advisable with 5.2-6.8.35cm is ploughed deeply, rejects impurity in furrow, careful site preparation, the wide 1.0m-1.2m of furrow, the high 20cm of furrow, furrow face is slightly
Low dip avoids ponding.Paris polyphylla seedling dedicated substrate 1000kg is applied per acre as base fertilizer, uniformly sprinkles one layer in furrow face
Matrix, then flattened plot with spike-tooth harrow, wait plant.
6th step is burrowed, with the whole plate punch of deep 2cm, seeding row spacing 3cm х 3cm January in whole good nursery field
Part by the 4th step, treated that the program request of paris polyphylla seed is being accomplished fluently in cave, is then covered with fertile soil, not reveal seed
It is advisable, it is thick is no more than seed face 1cm, then cover one layer of 1-2cm thickness pine needle, irrigates root water.
7th step carries out light transmission and sunshade work, keeps light transmittance 40%, mid-February, paris polyphylla seed started to grow
Leaf emergence, from seed collection to come into leaves emerge when it is 4 months a length of.
Experimental result is shown, until the emergence rate of late March paris polyphylla seed is 63%.
Embodiment 4
The first step, pericarp cracking is selected October, pericarp turns yellow, mature paris polyphylla seed and is put into 5-8 DEG C, low
Temperature processing 10-15 days；
Second step will complete low-temperature treatment paris polyphylla seed capsules and peel off shell, fine sand and seed are mixed by 2:1
It closes, red skin is removed in stranding；
Third step, fine sand were mixed thoroughly, moisturizing by fine sand with the seed 5:1 for removing kind of skin with 8 hours of 100 DEG C of high-temperature sterilizations,
Water content is about 50%, sets 18-20 DEG C, promotees bud and handles 40-50 days；
4th step is mixed seed after promoting bud with 600mg/L gibberellin, is slightly dried；
5th step turns over whole nursery field, selects abundant, loose, draining good permeability the sandy loam of the content of organic matter, soil pH
Value is advisable with 5.2-6.8.50cm is ploughed deeply, rejects impurity in furrow, careful site preparation, the wide 1.0m-1.2m of furrow, the high 23cm of furrow, furrow face is slightly
Low dip avoids ponding.Paris polyphylla seedling dedicated substrate 1000kg is applied per acre as base fertilizer, uniformly sprinkles one layer in furrow face
Matrix, then flattened plot with spike-tooth harrow, wait plant.
6th step is burrowed, with the whole plate punch of deep 3cm, seeding row spacing 3cm х 3cm January in whole good nursery field
Part by the 4th step, treated that the program request of paris polyphylla seed is being accomplished fluently in cave, is then covered with fertile soil, not reveal seed
It is advisable, it is thick is no more than seed face 1cm, then cover one layer of 1-2cm thickness pine needle, irrigates root water.
7th step carries out light transmission and sunshade work, keeps light transmittance 50%, mid-February, paris polyphylla seed started to grow
Leaf emergence, from seed collection to come into leaves emerge when it is 4 months a length of.
Experimental result is shown, until the emergence rate of late March paris polyphylla seed is 66%.
Embodiment 5
The first step, pericarp cracking is selected October, pericarp turns yellow, mature paris polyphylla seed and is put into 5-8 DEG C, low
Temperature processing 10-15 days；
Second step will complete low-temperature treatment paris polyphylla seed capsules and peel off shell, fine sand and seed are mixed by 2:1
It closes, red skin is removed in stranding；
Third step, fine sand were mixed thoroughly, moisturizing by fine sand with the seed 5:1 for removing kind of skin with 4 hours of 120 DEG C of high-temperature sterilizations,
Water content is about 50%, sets 22 DEG C, promotees bud and handles 60-70 days；
4th step is mixed seed after promoting bud with 600mg/L gibberellin, is slightly dried；
5th step turns over whole nursery field, selects abundant, loose, draining good permeability the sandy loam of the content of organic matter, soil pH
Value is advisable with 5.2-6.8.30cm is ploughed deeply, impurity in furrow, careful site preparation, the wide 1.0m-1.2m of furrow, the high 20cm-25cm of furrow, furrow are rejected
Face is slightly tilted, and avoids ponding.Paris polyphylla seedling dedicated substrate 1000kg is applied per acre as base fertilizer, is uniformly sprinkled in furrow face
One layer of matrix, then flattened plot with spike-tooth harrow, wait plant.
6th step is burrowed, with the whole plate punch of deep 1cm, seeding row spacing 3cm х 3cm January in whole good nursery field
The middle ten days, treated that the program request of paris polyphylla seed is being accomplished fluently in cave by the 4th step, is then covered with fertile soil, not reveal kind
Son is advisable, and it is thick to be no more than seed face 1cm, then cover one layer of 1-2cm thickness pine needle, irrigates root water.
7th step carries out light transmission and sunshade work, keeps light transmittance 60%, mid-February, paris polyphylla seed started to grow
Leaf emergence, from seed collection to come into leaves emerge when it is 3 months a length of.
Experimental result is shown, until the emergence rate of late March paris polyphylla seed is 58%.
Embodiment 6
The first step, pericarp cracking is selected October, pericarp turns yellow, mature paris polyphylla seed and is put into 5-8 DEG C, low
Temperature processing 10-15 days；
Second step will complete low-temperature treatment paris polyphylla seed capsules and peel off shell, fine sand and seed are mixed by 2:1
It closes, red skin is removed in stranding；
Third step, fine sand were mixed thoroughly, moisturizing by fine sand with the seed 5:1 for removing kind of skin with 6 hours of 105 DEG C of high-temperature sterilizations,
Water content is about 50%, sets 22 DEG C, promotees bud and handles 40-50 days；
4th step turns over whole nursery field, selects abundant, loose, draining good permeability the sandy loam of the content of organic matter, soil pH
Value is advisable with 5.2-6.8.30cm or more is ploughed deeply, impurity in furrow, careful site preparation, the wide 1.0m-1.2m of furrow, the high 20cm- of furrow are rejected
25cm, furrow face are slightly tilted, and avoid ponding.Paris polyphylla seedling dedicated substrate 1000kg is applied per acre as base fertilizer, in furrow face
One layer of matrix uniformly is sprinkled, then is flattened plot with spike-tooth harrow, wait plant.
5th step is burrowed, with the whole plate punch of deep 2cm, seeding row spacing 3cm х 3cm January in whole good nursery field
Part by the 4th step, treated that the program request of paris polyphylla seed is being accomplished fluently in cave, is then covered with fertile soil, not reveal seed
It is advisable, it is thick is no more than seed face 1cm, then cover one layer of 1-2cm thickness pine needle, irrigates root water.
6th step carries out light transmission and sunshade work, keeps light transmittance 40%, mid-February, paris polyphylla seed started to grow
Leaf emergence, from seed collection to come into leaves emerge when it is 4 months a length of.
Experimental result is shown, until the emergence rate of late March paris polyphylla seed is 69%.It can be seen that seed does not have
It is stimulated by plant hormone, emergence rate is lower.
Embodiment 7
The first step, pericarp cracking is selected October, pericarp turns yellow, mature paris polyphylla seed and is put into 5-8 DEG C, low
Temperature processing 10-15 days；
Second step will complete low-temperature treatment paris polyphylla seed capsules and peel off shell, fine sand and seed are mixed by 2:1
It closes, red skin is removed in stranding；
Third step, fine sand were mixed thoroughly, moisturizing by fine sand with the seed 5:1 for removing kind of skin with 6 hours of 105 DEG C of high-temperature sterilizations,
Water content is about 50%, sets 22 DEG C, promotees bud and handles 40-50 days；
4th step is mixed seed after promoting bud with 400mg/L gibberellin, is slightly dried；
5th step turns over whole nursery field, selects abundant, loose, draining good permeability the sandy loam of the content of organic matter, soil pH
Value is advisable with 5.2-6.8.30cm or more is ploughed deeply, impurity in furrow, careful site preparation, the wide 1.0m-1.2m of furrow, the high 20cm- of furrow are rejected
25cm, furrow face are slightly tilted, and avoid ponding.Paris polyphylla seedling dedicated substrate 1000kg is applied per acre as base fertilizer, in furrow face
One layer of matrix uniformly is sprinkled, then is flattened plot with spike-tooth harrow, wait plant.
6th step is burrowed, with the whole plate punch of deep 2cm, seeding row spacing 3cm х 3cm January in whole good nursery field
Part by the 4th step, treated that the program request of paris polyphylla seed is being accomplished fluently in cave, is then covered with fertile soil, not reveal seed
It is advisable, it is thick is no more than seed face 1cm, then cover one layer of 1-2cm thickness pine needle, irrigates root water.
7th step carries out light transmission and sunshade work, keeps light transmittance 40%, mid-February, paris polyphylla seed started to grow
Leaf emergence, from seed collection to come into leaves emerge when it is 4 months a length of.
Experimental result is shown, until the emergence rate of late March paris polyphylla seed is 75%.
Embodiment 8
The first step, pericarp cracking is selected October, pericarp turns yellow, mature paris polyphylla seed and is put into 5-8 DEG C, low
Temperature processing 10-15 days；
Second step will complete low-temperature treatment paris polyphylla seed capsules and peel off shell, fine sand and seed are mixed by 2:1
It closes, red skin is removed in stranding；
Third step, fine sand were mixed thoroughly, moisturizing by fine sand with the seed 5:1 for removing kind of skin with 6 hours of 105 DEG C of high-temperature sterilizations,
Water content is about 50%, sets 22 DEG C, promotees bud and handles 40-50 days；
4th step is mixed seed after promoting bud with 800mg/L gibberellin, is slightly dried；
5th step turns over whole nursery field, selects abundant, loose, draining good permeability the sandy loam of the content of organic matter, soil pH
Value is advisable with 5.2-6.8.30cm or more is ploughed deeply, impurity in furrow, careful site preparation, the wide 1.0m-1.2m of furrow, the high 20cm- of furrow are rejected
25cm, furrow face are slightly tilted, and avoid ponding.Paris polyphylla seedling dedicated substrate 1000kg is applied per acre as base fertilizer, in furrow face
One layer of matrix uniformly is sprinkled, then is flattened plot with spike-tooth harrow, wait plant.
6th step is burrowed, with the whole plate punch of deep 2cm, seeding row spacing 3cm х 3cm January in whole good nursery field
Part by the 4th step, treated that the program request of paris polyphylla seed is being accomplished fluently in cave, is then covered with fertile soil, not reveal seed
It is advisable, it is thick is no more than seed face 1cm, then cover one layer of 1-2cm thickness pine needle, irrigates root water.
7th step carries out light transmission and sunshade work, keeps light transmittance 40%, mid-February, paris polyphylla seed started to grow
Leaf emergence, from seed collection to come into leaves emerge when it is 4 months a length of.
Experimental result is shown, until the emergence rate of late March paris polyphylla seed is 80%.
Embodiment 9
The first step, pericarp cracking is selected October, pericarp turns yellow, mature paris polyphylla seed and is put into 5-8 DEG C, low
Temperature processing 10-15 days；
Second step will complete low-temperature treatment paris polyphylla seed capsules and peel off shell, fine sand and seed are mixed by 2:1
It closes, red skin is removed in stranding；
Third step, fine sand were mixed thoroughly, moisturizing by fine sand with the seed 5:1 for removing kind of skin with 6 hours of 105 DEG C of high-temperature sterilizations,
Water content is about 50%, sets 22 DEG C, promotees bud and handles 40-50 days；
4th step is mixed seed after promoting bud with 600mg/L gibberellin, is slightly dried；
5th step turns over whole nursery field, selects abundant, loose, draining good permeability the sandy loam of the content of organic matter, soil pH
Value is advisable with 5.2-6.8.30cm or more is ploughed deeply, impurity in furrow, careful site preparation, the wide 1.0m-1.2m of furrow, the high 20cm- of furrow are rejected
25cm, furrow face are slightly tilted, and avoid ponding.Paris polyphylla seedling dedicated substrate 1000kg is applied per acre as base fertilizer, in furrow face
One layer of matrix uniformly is sprinkled, then is flattened plot with spike-tooth harrow, wait plant.
6th step is burrowed, with the whole plate punch of deep 2cm, seeding row spacing 3cm х 3cm in 2 months in whole good nursery field
The middle ten days, treated that the program request of paris polyphylla seed is being accomplished fluently in cave by the 4th step, is then covered with fertile soil, not reveal kind
Son is advisable, and it is thick to be no more than seed face 1cm, then cover one layer of 1-2cm thickness pine needle, irrigates root water.
7th step carries out light transmission and sunshade work, keeps light transmittance 40%.
Experimental result is shown, until the emergence rate of late March paris polyphylla seed is 26%.After mid-February, that is, the beginning of spring
Sowing, seed does not pass through natural low temperature, is difficult to emerge for the year, so emergence rate is very low.
Embodiment 10
The first step, pericarp cracking is selected October, pericarp turns yellow, mature paris polyphylla seed and is put into 5-8 DEG C, low
Temperature processing 10-15 days；
Second step will complete low-temperature treatment paris polyphylla seed capsules and peel off shell, fine sand and seed are mixed by 2:1
It closes, red skin is removed in stranding；
Third step, fine sand were mixed thoroughly, moisturizing by fine sand with the seed 5:1 for removing kind of skin with 6 hours of 105 DEG C of high-temperature sterilizations,
Water content is about 50%, sets 22 DEG C, promotees bud and handles 40-50 days；
4th step is mixed seed after promoting bud with 600mg/L gibberellin, is slightly dried；
5th step turns over whole nursery field, selects abundant, loose, draining good permeability the sandy loam of the content of organic matter, soil pH
Value is advisable with 5.2-6.8.30cm or more is ploughed deeply, impurity in furrow, careful site preparation, the wide 1.0m-1.2m of furrow, the high 20cm- of furrow are rejected
25cm, furrow face are slightly tilted, and avoid ponding.Paris polyphylla seedling dedicated substrate 1000kg is applied per acre as base fertilizer, in furrow face
One layer of matrix uniformly is sprinkled, then is flattened plot with spike-tooth harrow, wait plant.
6th step is burrowed, with the whole plate punch of deep 2cm, seeding row spacing 3cm х 3cm January in whole good nursery field
Part by the 4th step, treated that the program request of paris polyphylla seed is being accomplished fluently in cave, is then covered with fertile soil, thickness of earth covering is
2cm-3cm on seed face, then one layer of 1-2cm thickness pine needle is covered, irrigate root water.
7th step carries out light transmission and sunshade work, keeps light transmittance 40%, mid-February, paris polyphylla seed started to grow
Leaf emergence, from seed collection to come into leaves emerge when it is 4 months a length of.
Experimental result is shown, until the emergence rate of late March paris polyphylla seed is 45%.
To sum up, the selection of temperature control, plant hormone stimulation and sowing time, is to guarantee paris polyphylla seed sprouting
The key emerged in rate and First Year also ensures that paris polyphylla has provenance to carry out large-scale plantation, protects in imminent danger wild
Resource.
It should be noted that being not intended to limit although the various embodiments described above have been described herein
Scope of patent protection of the invention.Therefore, it based on innovative idea of the invention, change that embodiment described herein is carried out and is repaired
Change, or using equivalent structure or equivalent flow shift made by description of the invention and accompanying drawing content, it directly or indirectly will be with
Upper technical solution is used in other related technical areas, is included within scope of patent protection of the invention.
Claims (6)
1. a kind of mating system that paris polyphylla seed quickly comes into leaves, it is characterised in that: the mating system is to pass through change
The collective effect of mild plant hormone, promotes seed to sprout；Then by the variation of temperature from low to high, promote the seed sprouted
It comes into leaves.
2. the mating system that paris polyphylla seed according to claim 1 quickly comes into leaves, it is characterised in that: described is numerous
Method is educated the following steps are included: low-temperature treatment paris polyphylla seed；Shell is peelled off, red skin is removed；Moisture-heat preservation promotees bud processing
Seed；It is sprouted with plant hormone stimulation seed；Seed is put be sowed at nursery Tanaka at low temperature；Control illumination waits emerging.
3. the mating system that paris polyphylla seed according to claim 1 or 2 quickly comes into leaves, it is characterised in that: described
Plant hormone include gibberellin.
4. the mating system that paris polyphylla seed according to claim 1 to 3 quickly comes into leaves, it is characterised in that:
Specific step is as follows for the mating system:
The first step selects pericarp cracking, pericarp turns yellow, mature paris polyphylla seed and is put into 2-8 DEG C, low-temperature treatment 10-15
It；
The paris polyphylla seed capsules for completing low-temperature treatment are peelled off shell by second step, and fine sand and seed are mixed by 1-3:1
It closes, red skin is removed in stranding；
Third step, fine sand were mixed thoroughly by fine sand with the seed 4-6:1 for removing kind of skin with 4-8 hour of 100-120 DEG C of high-temperature sterilization,
Moisturizing, water content 40-60% set 18-22 DEG C, promote bud and handle 40-50 days；
4th step is mixed seed after promoting bud with 400-800mg/L gibberellin, is slightly dried；
5th step is turning over whole good nursery field, with the whole plate punch of deep 1-3cm, seeding row spacing 3cm х 3cm, is burrowing, will be upper
Stating treated, seed program request under natural low temperature is covered in accomplishing fluently cave with fertile soil, is advisable with not revealing seed, and do not surpass
1-3cm on seed face is crossed, then covers one layer of 1-2cm thickness pine needle, irrigates root water；
6th step carries out light transmission and sunshade work, keeps light transmittance 30-50%, seed is allowed to tide over 0 DEG C -22 DEG C in nursery
Natural temperature variation, paris polyphylla seed starts emergence of coming into leaves after 1-2 months.
5. the mating system that paris polyphylla seed according to claim 4 quickly comes into leaves, it is characterised in that: described educates
The adjusting method that turns in seedling field is to select abundant, loose, draining good permeability the sandy loam of the content of organic matter, and soil pH value is with 5.2-
6.8 being advisable；30cm or more is ploughed deeply, rejects impurity in furrow, careful site preparation, the wide 1.0-1.2m of furrow, the high 20-25cm of furrow, furrow face is slightly
Inclination, avoids ponding；One layer of matrix is uniformly sprinkled in furrow face, flattens plot.
6. the mating system that paris polyphylla seed according to claim 4 quickly comes into leaves, it is characterised in that: the kind
Son program request under natural low temperature specifically: be January before the beginning of spring, environment temperature is sowed at 0 DEG C -5 DEG C.
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| CN114223471A (en) * | 2021-10-19 | 2022-03-25 | 重庆师范大学 | Rapid seedling method of paris polyphylla |
| CN114902831A (en) * | 2022-05-25 | 2022-08-16 | 湖南省农业环境生态研究所 | Rapid germination accelerating method for promoting paris polyphylla seed germination |
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| CN114902831A (en) * | 2022-05-25 | 2022-08-16 | 湖南省农业环境生态研究所 | Rapid germination accelerating method for promoting paris polyphylla seed germination |
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Application publication date: 20190111 Assignee: FUJIAN CHENGTIAN AGRICULTURE AND FORESTRY TECHNOLOGY DEVELOPMENT CO.,LTD. Assignor: AGRICULTURAL BIORESOURCES INSTITUTE OF FUJIAN ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2022350000018 Denomination of invention: A breeding method for rapid leaf growth of seven leaf and one branch flower seeds Granted publication date: 20210813 License type: Common License Record date: 20220705 |
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Effective date of registration: 20231214 Address after: Comprehensive Experimental Building (Crop Institute), No. 104 Pudang Road, Xindian Town, Jin'an District, Fuzhou City, Fujian Province, 350013 Patentee after: Crop Research Institute of Fujian Academy of Agricultural Sciences (Fujian Provincial Germplasm Resources Center) Address before: Room 1309, high tech building, Academy of Agricultural Sciences, 247 Wusi Road, Gulou District, Fuzhou, Fujian 350003 Patentee before: AGRICULTURAL BIORESOURCES INSTITUTE OF FUJIAN ACADEMY OF AGRICULTURAL SCIENCES |