CN107810958B - Method for promoting paris polyphylla seed germination - Google Patents
Method for promoting paris polyphylla seed germination Download PDFInfo
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- CN107810958B CN107810958B CN201710977313.6A CN201710977313A CN107810958B CN 107810958 B CN107810958 B CN 107810958B CN 201710977313 A CN201710977313 A CN 201710977313A CN 107810958 B CN107810958 B CN 107810958B
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- fluazinone
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
Abstract
The invention discloses a method for promoting paris polyphylla seed germination. The invention provides a paris polyphylla seed germination method, which comprises the following steps: 1) treating the paris polyphylla seeds with a plant growth regulator or a plant growth regulator solution to obtain treated seeds; the solute of the plant growth regulator solution or the plant growth regulator is gibberellin and fluazinone; 2) and placing the treated seeds in a water-containing river sand germination box, and performing germination culture to realize the germination of the paris polyphylla seeds. The invention completes the morphological after-ripening process under the condition of high temperature (20 ℃) by using temperature stratification combined with hormone treatment, and further uses gibberellin and fluazinone to break physiological dormancy, so that the germination rate of the paris polyphylla reaches more than 95 percent in 60 days; compared with the existing paris polyphylla seed germination method, the seed germination time is obviously shortened, and the germination rate is obviously improved.
Description
Technical Field
The invention relates to the technical field of seed biology, in particular to a method for promoting paris polyphylla seed germination.
Background
Parisphyllum rhizome [ Parispyphyllia Smith var. yunnanensis (Franch.) hand. -Mzt. ] is a perennial herb of Paris genus of Liliaceae family. The rootstock is thick and obvious in nodulation, and has scaly leaves and numerous fibrous roots. The stem is 20-100cm high, has no hair, and is purple red, and the base has 1-3 pieces of tunica blade sheath phimosis stem. 5-11 rotarily-grown leaves, which are oval-shaped and long or needle-shaped, sharp or tapered at the tip, wedge-shaped to round at the base, complete edge, and have 3 obvious base pulse, the length of the leaf is 7-17cm, and the width is 2.2-6 cm. The flower single stem top is in amphiprotic state, the flower quilt is made of two wheels, the outer wheel quilt sheet is 4-7 in green, the number of the inner wheel quilt sheet and the outer wheel quilt sheet is the same, and the flower single stem top is yellow or yellow-green, linear and 2-5mm wide. 2-4 rounds of stamens, obvious drug separation, and 1 chamber of ovary. The fruits are nearly spherical green, are irregularly cracked, have most seeds and are egg-spherical, and have bright red episperms. The medicinal part is rhizome, has the efficacies of clearing away heat and toxic material, relieving swelling and pain, and cooling liver and arresting convulsion, and is recorded in Shen nong's herbal Jing. The main medicinal component of Yunnan Baiyao, Jideshe medicine and Gongxuening is Yunnan manyleaf Paris rhizome. In addition, the extract has obvious effects in the aspects of resisting tumors, regulating immunity, inhibiting bacteria, diminishing inflammation and the like.
Under natural conditions, the breeding mode of the paris polyphylla is seed breeding and tuber division breeding, the seedling rate of the tuber division breeding is limited, the breeding coefficient is small, the seed breeding has the physiological characteristic of secondary dormancy, the seedling can be grown in 2 years under natural conditions, the resource regeneration period is long, and the utilization of medicinal materials is greatly restricted.
For a long time, the raw materials of the paris polyphylla almost all depend on wild resources, and along with the continuous and rapid increase of domestic and foreign requirements, the wild resources are rapidly reduced due to the random excavation, and the seedling raising technology of the paris polyphylla becomes a problem to be solved urgently.
The paris polyphylla seeds belong to a morphological physiological shape dormancy type, the embryo of the seeds which are just harvested is not completely developed, the seeds can not germinate after being subjected to morphological post-maturation for a period of time and developed into mature embryos, the seeds can germinate after being subjected to physiological post-maturation by cold (0-10 ℃) or warm (15 ℃) lamination for a period of time.
The Yunnan Paris polyphylla seedling raising method is characterized in that a great amount of research is carried out on seedling raising of Yunnan Paris polyphylla at present by scholars at home, Li Yunchang considers that the Yunnan Paris polyphylla can sprout only after fifteen months under natural conditions and the rate of emergence is only 46.2%, the germination rate of the Yunnan Paris polyphylla seeds can reach more than 60% within 12 months by using two low-temperature stratification combined with middle-stage high-temperature treatment, Yuanliangchun and other people consider that the temperature is the most important factor for secondary development of the Yunnan Paris seeds and the optimal temperature is 18-20 ℃ and 18 ℃, the initial emergence time of radicle is 43 days and the rooting rate of germination is 80% is 61 days.
Combining the research results, the dormancy of the paris polyphylla seeds is broken by mainly applying low-temperature stratification combined hormone treatment. The germination of the paris polyphylla seeds is basically over 60 days, and the germination rate is not ideal.
Disclosure of Invention
The invention aims to provide a paris polyphylla seed germination method.
The method provided by the invention comprises the following steps:
1) treating the paris polyphylla seeds with gibberellin and fluazinone or with a solution containing the gibberellin and the fluazinone to obtain treated seeds;
2) and (4) germinating and culturing the treated seeds to realize the germination of the paris polyphylla seeds.
In the above-mentioned method, the first step of the method,
the mass ratio of the gibberellin to the fluazinone is 50-200: 1-10;
or the mass ratio of the gibberellin to the fluazinone is 100: 5 or 100: 1.
In the above-mentioned method, the first step of the method,
in the solution containing gibberellin and fluazinone, the concentration of the gibberellin is 50-200 mg/L, and the concentration of the fluazinone is 1-10 mg/L;
or in the solution containing gibberellin and fluazinone, the concentration of the gibberellin is 100 mg/L, and the concentration of the fluazinone is 5 mg/L or 1 mg/L;
or the solvent in the solution containing gibberellin and fluazinone is water.
In the above-mentioned method, the first step of the method,
the treatment is to soak the paris polyphylla seeds in a solution containing gibberellin and fluazinone.
In the above-mentioned method, the first step of the method,
the time of the treatment is 6-48h,
or the treatment time is specifically 24 h.
In the above method, the temperature of the germination culture is 20 ℃.
In the method, the humidity of the germination culture is 60-80%,
or the germination culture mode is to smash seeds once every 5-8 days.
The germination culture is to place the treated seeds in a water-containing river sand germination box, and specifically comprises the following steps: firstly, paving a layer of water-containing river sand at the bottom of a germination box, and then uniformly mixing and paving the treated seeds and the water-containing river sand; finally, paving a layer of the water-containing river sand; the mass ratio of the treated seeds to the river sand is 1:10 when the seeds and the river sand are uniformly mixed and paved.
The method also comprises the following steps before the treatment: and sequentially removing the testa of the paris polyphylla seeds and sterilizing.
The application of gibberellin and fluazinone in promoting the germination of paris polyphylla seeds is also within the protection scope of the invention.
In the application, the use concentration of the gibberellin for promoting the germination of the paris polyphylla seeds is 50-200 mg/L, and the use concentration of the fluazinone for promoting the germination of the paris polyphylla seeds is 1-10 mg/L;
or the use concentration of the gibberellin for promoting the germination of the paris polyphylla seeds is 100 mg/L, and the use concentration of the fluazinone for promoting the germination of the paris polyphylla seeds is 1 mg/L or 5 mg/L.
The invention also aims to provide a paris polyphylla seed germination product.
The product provided by the invention comprises the gibberellin and the fluazinone treatment or the solution containing the gibberellin and the fluazinone.
Aiming at the characteristics of the morphological physiological dormancy of the paris polyphylla, and combining the research results of predecessors, the morphological after-ripening process is completed under the condition of high temperature (20 ℃) by using temperature stratification and hormone treatment, and then the physiological dormancy is broken through gibberellin and fluazinone, so that the germination rate of the paris polyphylla reaches more than 95 percent in 60 days; compared with the existing paris polyphylla seed germination method, the seed germination time is obviously shortened, the germination rate is obviously improved, and the method has the advantages of simple operation and rapid and orderly germination.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1 Paris polyphylla seed germination method
Seed germination of rhizoma paridis Yunnanensis
1. Selecting seeds with uniform seed size and maturity
The mature and well-developed paris polyphylla seed episperm is dark red and has plump berry-shaped juice.
Removing the testa of the collected ripe Yunnan manyleaf Paris rhizome by kneading, thoroughly washing off the testa and the residual color on the testa with flowing water to make the testa appear white or milky white of the ripe seeds, drying in the shade, and selecting seeds with uniform size and maturity for later use.
2. Disinfection
And (3) disinfecting the seeds treated by the step (1) by using a sodium hypochlorite aqueous solution with the concentration of 1% for 20min, and washing the seeds with sterile distilled water for 5 times to obtain the disinfected seeds.
3. Break the physiological dormancy of seeds
The disinfected seeds obtained in the step 2 are respectively treated according to the following groups, and the seeds are stirred for a plurality of times during the treatment, so that the normal breathing of the seeds is ensured:
and (3) CK group: soaking the disinfected seeds in distilled water (CK) at normal temperature for 24 h;
flu1 group, the disinfected seeds are soaked in 1 mg/L aqueous solution of fluazinone for 24 hours at normal temperature;
flu5 group, the disinfected seeds are soaked in 5 mg/L aqueous solution of fluazinone for 24 hours at normal temperature;
flu10 group, the disinfected seeds are soaked in 10 mg/L aqueous solution of fluazinone for 24 hours at normal temperature;
GA100 group, sterile seed 100 mg/L gibberellin GA3Soaking the water solution for 24h at normal temperature;
flu1+ GA100 group, sterilized seeds were treated with gibberellin GA at a final concentration of 100 mg/L3And a final concentration of 1 mg/L Fluazinone (gibberellin GA)3And an aqueous solution of fluazinone dissolved in water, wherein the solute content is 100 mg/L gibberellin GA3And 1 mg/L of fluazinone), and soaking for 24 hours at normal temperature;
flu5+ GA100 group, sterilized seeds were treated with gibberellin GA at a final concentration of 100 mg/L3And a final concentration of 5 mg/L of fluazinone (gibberellin GA)3And an aqueous solution of fluazinone dissolved in water, wherein the solute content is 100 mg/L gibberellin GA3And 5 mg/L fluazinone), soaking for 24h at normal temperature;
flu10+ GA100 group, sterilized seeds were treated with gibberellin GA at a final concentration of 100 mg/L3And a final concentration of 10 mg/L of fluazinone (gibberellin GA)3And an aqueous solution of fluazinone dissolved in water, wherein the solute content is 100 mg/L gibberellin GA3And 10 mg/L fluazinone), and soaking for 24h at normal temperature.
The total volume of the seeds should be no greater than 1/5 of the total volume of the solution.
Three biological repetitions are carried out respectively to obtain the soaked seeds.
3. Germinating and germinating
1) Preparing water-containing river sand germination box
Drying the river sand at 121 ℃ for 3h, and preparing the water-containing river sand according to the mass ratio of the river sand to the sterilized distilled water of 5: 1.
2) Germinating box with seeds
Spreading a layer of water-containing river sand with the thickness of about 2cm at the bottom of the germination box, uniformly mixing 300 soaked seeds of the group obtained in the step 2 according to the mass ratio of the seeds to the water-containing river sand of 1:10, spreading the mixture on the water-containing river sand with the thickness of about 2cm, and finally spreading a layer of water-containing river sand with the thickness of about 2cm to obtain the germination box containing the seeds.
In the treatment process, the hydrous river sand is not required to be compacted, the good ventilation state of the sand is kept to ensure the normal respiration of the seeds, and the germination box cover is covered. The germination box can not be sealed by a sealing film, and the humidity in the germination box can be rapidly increased after the germination box is sealed, so that the seed respiration is not facilitated.
3) Germination culture
And (3) placing the germination boxes with the seeds in each group into an artificial constant temperature incubator at 20 ℃ for germination and culture.
The humidity is kept at 60-80% in the culture, and the seeds are pounded once every five days, so that the normal water holding capacity and oxygen content of the wet sand are ensured.
Second, detecting
1. Method for determining optimal hormone treatment mode by detecting germination rate of paris polyphylla seeds
After the above-mentioned one was cultured in an artificial incubator for 45 days and 60 days, the germination rates of the respective groups were counted.
Germination rate ═ 100% (number of germinated seeds/total number of seeds)%
The results of germination rates at 20 ℃ with different hormone treatments are shown in table 1.
Table 1 shows the germination percentage (%) -of Paris polyphylla processed with different hormones at 20 deg.C
From the above, it can be seen that 45 days after the treatment, the germination rate of the control group is only 62%, while in Flu5+ GA100, the germination rate reaches 85%, and in Flu1+ GA100, the germination rate reaches 83%, and the germination rate after the regulator treatment is obviously improved, and the difference is obvious, which indicates that the plant growth regulator treatment has obvious effect of relieving dormancy. After 60 days of treatment, the germination rate of the control group was about 81%, the germination rate was as high as 97% in Flu5+ GA100, and the germination rate was 96% in Flu1+ GA 100. The optimal hormone treatment regimes were indicated as Flu5+ GA100 and Flu1+ GA 100.
2. Detecting germination rate at different temperatures and searching for optimal germination temperature
Placing the germination boxes with seeds of the CK group, the germination boxes with seeds of the Flu5+ GA100 group and the germination boxes with seeds of the Flu1+ GA100 group obtained in the step 3) into artificial constant temperature incubators at 15 ℃ for 12h/25 ℃ for 12h, 4 ℃ and 20 ℃ respectively for germination culture at different temperatures.
The results of measuring the germination rates of the control groups at different temperatures are shown in table 2.
Table 2 shows the germination rates of Paris polyphylla at different temperatures
As can be seen from the germination rate, the germination rate is highest at 20 ℃.
The embryo rate (embryo rate/endosperm length) of the control group at different temperatures was counted, using centrally longitudinally cut paris polyphylla seeds, photographed by observation with a stereomicroscope, and measured for length with image processing software, and the results of 40-50 seeds for each treatment are shown in table 3.
TABLE 3 embryo rate of Paris polyphylla in 30 days at different temperatures
The results show that the embryo culture rate is highest at 20 ℃.
Therefore, the temperature of 20 ℃ is the optimal germination temperature of the paris polyphylla seeds.
The above results indicate that the germination rate was significantly higher in 45 days than in the control group by culturing at 20 ℃ after adding the plant growth regulator (Flu1+ GA100 or Flu5+ GA 100), the physiological dormancy was completely released, and the Flu1+ GA100 and Flu5+ GA100 were treated and then cultured at 20 ℃ until they germinated substantially completely at 60 days, which were the optimal germination conditions.
Claims (3)
1. A paris polyphylla seed germination method comprises the following steps:
1) treating the paris polyphylla seeds with a solution containing gibberellin and fluopyridone to obtain treated seeds;
2) germinating and culturing the treated seeds to realize the germination of the paris polyphylla seeds;
the temperature of the germination culture is 20 ℃;
in the solution containing gibberellin and fluazinone, the concentration of the gibberellin is 100 mg/L, and the concentration of the fluazinone is 5 mg/L or 1 mg/L;
the solvent in the solution containing gibberellin and fluazinone is water;
the treatment is to soak the paris polyphylla seeds in a solution containing gibberellin and fluazinone;
the treatment time is 6-48 h;
the humidity of the germination culture is 60-80%,
the germination culture mode is that the seeds are poured once every 5 to 8 days.
2. The method of claim 1, further comprising:
the treatment time was 24 h.
3. Application of gibberellin and fluazinone in promoting germination of paris polyphylla seeds;
the application comprises the following steps:
1) treating the paris polyphylla seeds with a solution containing gibberellin and fluopyridone to obtain treated seeds;
2) germinating and culturing the treated seeds to realize the germination of the paris polyphylla seeds;
the temperature of the germination culture is 20 ℃;
in the solution containing gibberellin and fluazinone, the concentration of the gibberellin is 100 mg/L, and the concentration of the fluazinone is 5 mg/L or 1 mg/L;
the solvent in the solution containing gibberellin and fluazinone is water;
the treatment is to soak the paris polyphylla seeds in a solution containing gibberellin and fluazinone;
the treatment time is 6-48 h;
the humidity of the germination culture is 60-80%,
the germination culture mode is that the seeds are poured once every 5 to 8 days.
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| CN108811584A (en) * | 2018-06-26 | 2018-11-16 | 中国科学院成都生物研究所 | A method of promoting Paris polyphylla seed fast germination |
| CN111436437A (en) * | 2020-04-24 | 2020-07-24 | 中国科学院昆明植物研究所 | Reagent for antagonizing physiological action of n-butyl alcohol |
| CN112840971A (en) * | 2021-01-15 | 2021-05-28 | 甘肃省治沙研究所 | Method for rapidly improving seedling emergence of pioneer plant sand rice seeds of moving dune |
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| CN104472491B (en) * | 2014-12-10 | 2017-06-16 | 新疆林科院造林治沙研究所 | A kind of seed-soaking liquid for promoting Cistanche deserticola seed to sprout and preparation method thereof |
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