CN107567761A - A kind of method for improving iris seed and sprouting - Google Patents
A kind of method for improving iris seed and sprouting Download PDFInfo
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- CN107567761A CN107567761A CN201710969033.0A CN201710969033A CN107567761A CN 107567761 A CN107567761 A CN 107567761A CN 201710969033 A CN201710969033 A CN 201710969033A CN 107567761 A CN107567761 A CN 107567761A
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Abstract
The invention discloses a kind of method for improving iris seed and sprouting, pass through 6 BA, KNO3、H2O2In a kind of medicament to iris seed-soaking processing after sow, substantially increase the germination rate of seed, for the germ plasm resource of China iris, crossbreeding, expansion numerous and Landscape Application theoretical direction is provided.After testing, 6 BA, KNO3And H2O2Influence, and positive effect are remarkably promoted on improving chinensis seed sprouting, optimum concentration is respectively 300mg/L, 0.4%, 0.1%, and its germination rate is respectively 78.89%, 90.00%, 86.67%；6 BA and H2O2Raising sword-like iris seed seed, which is sprouted, to be had extremely notable or remarkably promotes influence, and optimum concentration is respectively 200mg/L, 0.4%, and its germination rate is respectively 93.33%, 50.00%.
Description
Technical field
The present invention relates to plant species field, and in particular to a kind of method for improving iris seed and sprouting.
Background technology
Iris is perennial herb plant, and China's germ plasm resource is enriched.Because its is various in style, adaptability
By force, large flower and brilliant color, flower pattern is peculiar, is one of excellent material of green planted city, it may also be used for medicine and feed, in cut-flower and gardens
Had a extensive future in greening.
Wild Iris plant mainly uses division propagation, but breeding coefficient is low, also using seed propagation, but most of kite
Tail seed has dormancy, emergence difficult, certain difficulty is brought to work such as iris resource introducing and planting, breedings, resistance
Its application in horticultural gardening, crossbreeding is hindered.A large amount of scholars are studied its seed dormancy, however, different kites
The Dormancy Cause of tail platymiscium seed is different, be broadly divided into the reason for seed dormancy kind of skin machinery hinder, a germination inhibitor
In the presence of, kind embryo after-ripening do not complete and external environmental condition limitation etc., mainly using illumination, temperature, lamination, medicament, In vitro Embryo
Its dormancy is broken in the processing such as culture and radiation treatment.
This research is with afforestation application prospectIt is wideIris species-Chinese small iris (I.lactea
Pall.var.chinensis Kodiz.), small stream sweet-smelling grass (I.sanguinea Donn.ex Horn.) and sword-like iris seed (I.ensata
Thunb. it is) examination material, chemicals treatment is carried out to it, it is intended to find out rational medicament kind and concentration, and then improve its seed
Germination and the effect for promoting growth of seedling, break the bottleneck for hindering Chinese Wild iris resource to introduce a fine variety utilization, for China
The plasm resource protection of iris, crossbreeding, seed expand numerous and Landscape Application and provide theoretical direction and technical support.
The content of the invention
To solve the above problems, the invention provides a kind of method for improving iris seed and sprouting, greatly improve
The germination rate of seed.
To achieve the above object, the technical scheme taken of the present invention is：
A kind of method for improving iris seed and sprouting, passes through 6-BA, KNO3、H2O2In a kind of medicament to iris
Sowed after the processing of platymiscium seed-soaking；Described 6-BA, KNO3、H2O2In a kind of medicament to iris seed soak
The dormancy of iris seed can directly be broken after kind processing, shorten its time required for sprouting and remarkably promote it and sprout
Hair.
Preferably, the iris seed is chinensis seed, is that 300mg/L 6-BA seed soaking 48h is laggard by concentration
Row sowing.
Preferably, the iris seed is chinensis seed, passes through the KNO that concentration of volume percent is 0.4%3Leaching
Sowed after kind 48h.
Preferably, the iris seed is chinensis seed, passes through the H that concentration of volume percent is 0.1%2O2Leaching
Sowed after kind 24h.
Preferably, the 6-BA that it is 200mg/L by concentration that the iris seed, which is sword-like iris seed seed, soaks seed after 48h
Sowed.
Preferably, the iris seed is the H that sword-like iris seed seed is 0.4% by concentration of volume percent2O2Leaching
Sowed after kind 24h.
The present invention have selected preferable iris method for treating seeds by testing sieve, substantially increase sprouting for seed
Hair rate, theoretical direction is provided for the germ plasm resource of China iris, crossbreeding, the numerous and Landscape Application of expansion.After testing, 6-
BA、KNO3And H2O2Influence, and positive effect are remarkably promoted on improving chinensis seed sprouting, optimum concentration is respectively 300mg/
L, 0.4%, 0.1%, its germination rate is respectively 78.89%, 90.00%, 86.67%；6-BA and H2O2To improving sword-like iris seed seed
Sprouting, which has, extremely significantly or remarkably promotes influence, and optimum concentration is respectively 200mg/L, 0.4%, its germination rate is respectively 93.33%,
50.00%.
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
Embodiment
Harvesting seed ripe then is test material.Chinese small iris and small stream sweet-smelling grass pick up from Agricultural University Of Shenyang flower and plant base, beautiful cicada
Flower picks up from Antu, Jilin Province Erdaobaihe River.
Experimental method
(1)GA3Processing：First fully soaked seed with distilled water, with concentration be respectively again then 50mg/L, 100mg/L, 200mg/
L, 300mg/L, 400mg/L, 500mg/L GA3Processing seed soaking 48h.Using distilled water seed soaking as control；
(2) 6-BA handles 100mg/L, 200mg/L, 300mg/L 6-BA seed soaking 48h；
(3)KNO3The KNO of processing 0.1%, 0.2%, 0.4%3Soak seed 48h；
(4)H2O2The H of processing 0.1%, 0.2%, 0.4%2O2Soak seed 24h；
Data determination and processing method：The temperature 30 (8h illumination) that seed is sprouted/20 DEG C (16h is dark), intensity of illumination
2400-2600lx.Kind of skin 1mm is exposed as sprouting standard using radicle.Counting germination rate, (Chinese small iris cultivates 90d, the training of remaining iris species
Support 30d, similarly hereinafter), vigor, germination index.Each 3 repetitions of processing, often repeatedly 30 seeds.Calculation formula is as follows：
Germination rate (%)=seed sprouting number/total seed number × sub- sprouting number
Sprouting number when vigor (%)=germination seed number peaks/seed sum × son sum ×
Germination index=count × peak (Gt is sprouting number in the t times, and Dt sprouts number of days to be corresponding) of bristling with anger
Experimental data application EXCEL softwares and the professional version softwares of SPSS 17.0 carry out variance analysis.
As a result with analysis
As it can be seen from table 1 various concentrations GA3Handle to the germination rate of small stream sweet-smelling grass seed, germination index influence difference not
Significantly, germination rate can reach more than 94.44% between each processing, and germination index can reach more than 2.32.But vigor is everywhere
There is some difference between reason, wherein GA3When concentration is 100mg/L, its vigor is maximum, is 80.00%.
Various concentrations GA3It is not notable to handle the germination rate to chinensis seed, the influence difference of germination index, is sprouted between each processing
Hair rate can reach more than 10.00%, and germination index can reach more than 0.06.But vigor exists certain poor between respectively handling
It is different, wherein GA3When concentration is 100mg/L, its vigor is maximum, is 32.22%.It can then suppress to plant when concentration is more than 300mg/L
Son is sprouted, and shows GA3Concentration is excessive, can suppress seed sprouting, illustrate GA3Unobvious are sprouted to improving chinensis seed.
Various concentrations GA3It is not notable to handle the germination rate to sword-like iris seed seed, the influence difference of vigor, is sprouted between each processing
Hair rate can reach more than 38.89%, and vigor can reach more than 26.67%.But germination index exists certain between respectively handling
Difference, wherein GA3When concentration is 300mg/L, its vigor is maximum, is 2.49, illustrates GA3Fail to understand improving chinensis seed and sprouting
It is aobvious.
1 different GA of table3The influence that concentration processing is sprouted to seed
DIG：Date of initial germination；GR：Germination rate；GE：Germination
energy；GI：Germination index.The same below.
Note：Different capitalizations represent difference extremely significantly (P ＜ 0.01), different lowercase letter indication differences significantly (P ＜
0.05), n=3, similarly hereinafter.
As known from Table 2, influence of the various concentrations 6-BA processing to the germination rate of small stream sweet-smelling grass seed, vigor, germination index is poor
Different not notable, each germination rate that handles can reach more than 95.56%, and vigor can reach more than 47.78%, and germination index is equal
More than 2.31 are can reach, illustrates that 6-BA is not notable to improving small stream sweet-smelling grass Seed Germination.
Chinensis seed after various concentrations 6-BA processing, germination rate, vigor between concentration 300mg/L and other processing,
Germination index significant difference is extremely notable, and concentration 300mg/L germination rate, vigor, germination index is maximum, is respectively
78.89%th, 44.45%, 0.40.As can be seen here, 6-BA remarkably promotes influence to improving chinensis seed sprouting, and optimum concentration is
300mg/L。
Various concentrations 6-BA processing is not notable to the influence difference of sword-like iris seed seed vigor, and each processing vigor is reachable
To more than 37.78%.But it is extremely notable to the influence difference of germination rate and germination index, when concentration is 100mg/L~300mg/L
When, germination rate and germination index all pole are significantly higher than control, and germination rate reaches more than 78.89%, and germination index reaches
More than 3.50.It can be seen that 6-BA, which produces pole to breaking sword-like iris seed seed dormancy, remarkably promotes influence, optimum concentration 200mg/L, its
Germination rate, vigor, germination index are respectively 93.33%, 50.00%, 4.36.
The influence that the various concentrations 6-BA of table 2 processing is sprouted to seed
As known from Table 3, various concentrations KNO3Handle, each processing not notable to the influence difference of small stream sweet-smelling grass germination rate, vigor
Germination rate reaches more than 90%, and vigor reaches more than 41.11%.But significant difference is influenceed on germination index, works as KNO3
When concentration is 0, its germination index is 2.39.As can be seen here, KNO3Effect is remarkably promoted to improving small stream sweet-smelling grass seed sprouting nothing.
Various concentrations KNO3Processing make chinensis seed beginning sprout number of days shorten 6~16 days, to its germination rate, vigor,
The influence difference of germination index is extremely notable, works as KNO3When concentration is 0.1%~0.4%, its germination rate, vigor, germination index
Pole is significantly higher than control, and its germination rate reaches more than 86.67%, and vigor reaches more than 62.22%, and germination index reaches
To more than 0.48, and when concentration is 0.4%, the equal highest of its germination rate, vigor, respectively 90.00%, 68.89%.Thus
It can be seen that KNO3There is pole to remarkably promote effect, optimum concentration 0.4% to improving chinensis seed and sprouting.
Various concentrations KNO3Processing is not notable to the influence difference of sword-like iris seed germination rate and germination index, and its germination rate reaches
To more than 37.78%, germination index reaches more than 1.91.But the influence difference to vigor is not notable, when concentration is 0.4%
When, the equal highest of its germination rate, vigor, germination index, respectively 55.56%, 48.89%, 2.66.As can be seen here, KNO3It is right
Improve sword-like iris seed seed sprouting without remarkably promote effect, optimum concentration 0.4%.
3 different KNO of table3The influence that concentration processing is sprouted to seed
As known from Table 4, various concentrations H2O2Handle to the germination rate of small stream sweet-smelling grass seed, vigor, germination index influence difference
Significantly, its germination rate does not reach more than 88.89%, and vigor reaches more than 30.0%, germination index reach 2.16 with
On, and when concentration is 0.2%, the equal highest of its germination rate, germination index, respectively 98.89%, 2.31.As can be seen here, H2O2
Effect is remarkably promoted to improving small stream sweet-smelling grass seed sprouting nothing.
Various concentrations H2O2Processing makes the beginning of chinensis seed sprout number of days delay 10~16 days.When concentration be 0.1%~
When 0.4%, its germination rate pole is significantly higher than control, and germination rate reaches more than 85.56%.As can be seen here, H2O2To improving Chinese small iris
Seed, which is sprouted, has pole to remarkably promote effect, optimum concentration 0.1%, and its germination rate, vigor, germination index are respectively
86.67%th, 61.11%, 0.50.
Various concentrations H2O2Processing makes the beginning of sword-like iris seed seed sprout number of days to shorten 3 days, to germination rate, germination index
Significant difference or extremely notable is influenceed, but the influence difference to its seed vigor is not notable.When concentration is 0.4%, it is sprouted
The equal highest of rate, vigor, germination index, respectively 50.00%, 30.00%, 2.66.As can be seen here, H2O2To improving sword-like iris seed
Seed, which is sprouted, remarkably promotes effect, optimum concentration 0.4%.
4 different H of table2O2The influence that concentration processing is sprouted to seed
In summary, 6-BA, KNO3、H2O2Effect is remarkably promoted to improving small stream sweet-smelling grass seed sprouting nothing；6-BA、KNO3And H2O2
Sprout on improving chinensis seed and remarkably promote influence, and positive effect, optimum concentration be respectively 300mg/L, 0.4%,
0.1%, its germination rate, vigor, germination index are respectively 78.89%, 44.45%, 0.40；90.00%th, 68.89%,
0.49；86.67%th, 61.11%, 0.50.6-BA and H2O2Raising sword-like iris seed seed, which is sprouted, to be had extremely notable or remarkably promotes shadow
Ring, optimum concentration is respectively 200mg/L, 0.4%, its germination rate, vigor, germination index are respectively 93.33%, 50.00%,
4.36；50.00%th, 30.00%, 2.66.But KNO3To improve sword-like iris seed seed sprouting without remarkably promote effect.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
- A kind of 1. method for improving iris seed and sprouting, it is characterised in that pass through 6-BA, KNO3、H2O2In a kind of medicine Agent after the processing of iris seed-soaking to sowing.
- A kind of 2. method for improving iris seed and sprouting as claimed in claim 1, it is characterised in that the 6-BA, KNO3、H2O2In a kind of medicament to can directly break the dormancy of iris seed after the processing of iris seed-soaking, Shorten its time required for sprouting and remarkably promote its sprouting.
- A kind of 3. method for improving iris seed and sprouting as claimed in claim 1, it is characterised in that the Jris Vegetable seeds is chinensis seed, is sowed after being 300mg/L 6-BA seed soaking 48h by concentration.
- A kind of 4. method for improving iris seed and sprouting as claimed in claim 1, it is characterised in that the Jris Vegetable seeds is chinensis seed, passes through the KNO that concentration of volume percent is 0.4%3Sowed after seed soaking 48h.
- A kind of 5. method for improving iris seed and sprouting as claimed in claim 1, it is characterised in that the Jris Vegetable seeds is chinensis seed, passes through the H that concentration of volume percent is 0.1%2O2Sowed after seed soaking 24h.
- A kind of 6. method for improving iris seed and sprouting as claimed in claim 1, it is characterised in that the Jris Vegetable seeds is sword-like iris seed seed, by concentration be 200mg/L 6-BA soak seed 48h after sowed.
- A kind of 7. method for improving iris seed and sprouting as claimed in claim 1, it is characterised in that the Jris Vegetable seeds is sword-like iris seed seed, passes through the H that concentration of volume percent is 0.4%2O2Sowed after seed soaking 24h.
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| CN201710969033.0A CN107567761B (en) | 2017-09-27 | 2017-09-27 | Method for improving seed germination of iris plants |
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| CN201710969033.0A CN107567761B (en) | 2017-09-27 | 2017-09-27 | Method for improving seed germination of iris plants |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110073760A (en) * | 2019-05-31 | 2019-08-02 | 兰州大学 | A kind of prewetting liquid quickly measured for Iris tenuifolia seed vigor, dyeing liquor and method |
| CN113099769A (en) * | 2021-03-30 | 2021-07-13 | 武汉市园林科学研究院 | Method for improving germination rate of acorus calamus |
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| CN101099422A (en) * | 2007-07-25 | 2008-01-09 | 毕慧滨 | Method for improving iris lactea seed germination rate |
| CN105638026A (en) * | 2016-01-11 | 2016-06-08 | 河北省林业科学研究院 | Method for breaking dormancy of iris barbata hybrid seeds |
| CN105684595A (en) * | 2016-01-19 | 2016-06-22 | 河北省林业科学研究院 | Method for breaking dormancy of hybrid seeds of bearded iris |
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2017
- 2017-09-27 CN CN201710969033.0A patent/CN107567761B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099422A (en) * | 2007-07-25 | 2008-01-09 | 毕慧滨 | Method for improving iris lactea seed germination rate |
| CN105638026A (en) * | 2016-01-11 | 2016-06-08 | 河北省林业科学研究院 | Method for breaking dormancy of iris barbata hybrid seeds |
| CN105684595A (en) * | 2016-01-19 | 2016-06-22 | 河北省林业科学研究院 | Method for breaking dormancy of hybrid seeds of bearded iris |
Non-Patent Citations (1)
| Title |
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| DAVID WEES 等: ""Stratification and priming may improve seed germination of purple coneflower，blue-flag Iris and evening primrose"", 《ACTA HORTICULTURAE 》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110073760A (en) * | 2019-05-31 | 2019-08-02 | 兰州大学 | A kind of prewetting liquid quickly measured for Iris tenuifolia seed vigor, dyeing liquor and method |
| CN110073760B (en) * | 2019-05-31 | 2020-07-24 | 兰州大学 | Pre-wetting solution, staining solution and method for rapidly determining vitality of Iris tenuifolia seeds |
| CN113099769A (en) * | 2021-03-30 | 2021-07-13 | 武汉市园林科学研究院 | Method for improving germination rate of acorus calamus |
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