CN107567761B - Method for improving seed germination of iris plants - Google Patents
Method for improving seed germination of iris plants Download PDFInfo
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Abstract
The invention discloses a method for improving iris plant seed germination by 6-BA and KNO3、H2O2The medicament in the iridaceae plant seed soaking. Detected 6-BA, KNO3And H2O2The Chinese iris seed germination can be obviously promoted, the effect is obvious, the optimal concentrations are respectively 300mg/L, 0.4% and 0.1%, and the germination rates are respectively 78.89%, 90.00% and 86.67%; 6-BA and H2O2Has extremely obvious or obvious promotion influence on improving the germination of the cordyceps sobolifera seeds, the optimal concentrations are respectively 200mg/L and 0.4 percent, and the germination rates are respectively 93.33 percent and 50.00 percent.
Description
Technical Field
The invention relates to the field of plant species, in particular to a method for improving seed germination of iris plants.
Background
The iris plants are perennial herbaceous plants, and the germplasm resources in China are rich. The flower is one of excellent materials for greening cities, can be used for medicines and feeds, and has wide prospects in cut flower and landscaping due to various varieties, strong adaptability, large and bright flowers and unique patterns.
The wild iris plants are mainly propagated by plants, but the propagation coefficient is low, and the wild iris plants can also be propagated by sowing, but most iris seeds have dormancy phenomenon and difficult seedling emergence, which brings certain difficulties for iris resource introduction cultivation, breeding and other works, and hinders the application of the wild iris plants in gardening cultivation and cross breeding. However, different iris plant seeds have different dormancy reasons, the dormancy reasons are mainly divided into mechanical obstruction of seed coats, existence of germination inhibitors, incomplete after-ripening of seed embryos, limitation of external environmental conditions and the like, and the dormancy is broken mainly by adopting treatments such as illumination, temperature, lamination, medicaments, in-vitro embryo culture, radiation treatment and the like.
According to the research, iris species, namely Chinese iris (I.lactea pall. var. chinensis Kodiz.), rabi (I.sanguinea donn. ex Horn.) and cicada flower (I.ensata Thunb.) which have wide landscaping application prospects are used as test materials, and the test materials are subjected to medicament treatment, so that reasonable medicament species and concentration are searched, the seed germination capacity of the iris species is improved, the seedling growth promoting effect of the iris is improved, the bottleneck of hindering the development and utilization of wild iris resource introduction of China is broken through, and theoretical guidance and technical support are provided for germplasm resource protection, cross breeding, seed propagation and garden application of iris plants of China.
Disclosure of Invention
In order to solve the problems, the invention provides a method for improving the germination of iris seeds, which greatly improves the germination rate of the seeds.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for improving seed germination of Iris plants by using 6-BA and KNO3、H2O2The preparation is prepared by soaking Iris plant seed in the preparation, and sowing; the 6-BA and KNO3、H2O2The one agent can directly break dormancy of iris seeds after the iris seeds are soaked, shorten the time required by the iris seeds to germinate and obviously promote the iris seeds to germinate.
Preferably, the iris plant seeds are Chinese iris seeds, and the seeds are sown after being soaked in 6-BA with the concentration of 300mg/L for 48 hours.
Preferably, the iris plant seeds are iris lactea seeds, and are obtained by passing through KNO with the volume percentage concentration of 0.4%3And (5) soaking the seeds for 48h, and then sowing.
Preferably, the iris plant seeds are iris lactea seeds, and the iris plant seeds are prepared by using 0.1% H by volume percentage concentration2O2And (5) soaking the seeds for 24h and then sowing.
Preferably, the Iris plant seeds are Isaria cicadae Miquel seeds, and the Iris plant seeds are sown after being soaked in 6-BA with the concentration of 200mg/L for 48 hours.
Preferably, the Iris plant seeds are Isaria cicadae Miq seeds with a volume percentage concentration of 0.4% of H2O2And (5) soaking the seeds for 24h and then sowing.
According to the invention, a better iris seed treatment method is screened out through tests, the germination rate of the seeds is greatly improved, and theoretical guidance is provided for germplasm resources, crossbreeding, propagation and garden application of iris plants in China. Detected 6-BA, KNO3And H2O2For improving the germination of Chinese iris seedHas obvious promotion effect, the optimal concentration is respectively 300mg/L, 0.4 percent and 0.1 percent, and the germination rate is respectively 78.89 percent, 90.00 percent and 86.67 percent; 6-BA and H2O2Has extremely obvious or obvious promotion influence on improving the germination of the cordyceps sobolifera seeds, the optimal concentrations are respectively 200mg/L and 0.4 percent, and the germination rates are respectively 93.33 percent and 50.00 percent.
Detailed Description
In order that the objects and advantages of the invention will be more clearly understood, the invention is further described in detail below with reference to examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Examples
Seeds that matured the year were harvested as the test material. Iris lactea and Lineara venosa are collected from Shenyang agriculture university flower base, and Iris cicada flower is collected from the second-channel Baihe of Antu county in Jilin province.
Experimental methods
(1)GA3And (3) treatment: soaking seeds in distilled water, and then respectively soaking with GA at concentrations of 50mg/L, 100mg/L, 200mg/L, 300mg/L, 400mg/L, and 500mg/L3And treating the soaked seeds for 48 hours. Soaking seeds in distilled water as a control;
(2)6-BA treating 100mg/L, 200mg/L and 300mg/L of 6-BA seed soaking for 48 hours;
(3)KNO3treating KNO of 0.1%, 0.2%, 0.4%3Soaking seeds for 48 h;
(4)H2O2treating 0.1%, 0.2%, 0.4% H2O2Soaking seeds for 24 hours;
the data measuring and processing method comprises the following steps: the temperature for seed germination was 30(8h light)/20 ℃ (16h dark), and the light intensity was 2400 and 2600 lx. The radicle is exposed 1mm of the seed coat as the standard of germination. And (4) counting the germination rate (culturing the iris lactea for 90 days, culturing the rest iris varieties for 30 days, and carrying out the same method below), the germination potential and the germination index. Each treatment was repeated 3 times, 30 seeds per repetition. The calculation formula is as follows:
the germination rate (%) is equal to the number of seeds germinated/total number of seeds × 100%
Germination potential (%) - (number of germinated seeds at the time when the number of germinated seeds reached the peak/total number of seeds X100%
Germination index ═ sigma (Gt/Dt) (Gt is germination number in t time, Dt is corresponding germination day number)
Experimental data analysis of variance was performed using EXCEL software and SPSS 17.0 professional version software.
Results and analysis
As can be seen from Table 1, the GA concentration varied3The difference of the influence of the treatment on the germination rate and the germination index of the rabdosia lophanthide seeds is not significant, the germination rate of each treatment interval can reach more than 94.44%, and the germination index can reach more than 2.32. However, there was some difference between treatments in germination vigour, where GA3The maximum germination potential is 80.00% at a concentration of 100 mg/L.
GA of different concentrations3The difference of the influence of the treatment on the germination rate and the germination index of the iris lactea seeds is not obvious, the germination rate can reach more than 10.00% in each treatment, and the germination index can reach more than 0.06. However, there was some difference between treatments in germination vigour, where GA3The maximum germination potential was 32.22% at a concentration of 100 mg/L. When the concentration is more than 300mg/L, the seed germination can be inhibited, which indicates that GA3The excessive concentration can inhibit seed germination, indicating that GA3The method is not obvious for improving the germination of the iris lactea seeds.
GA of different concentrations3The difference of the treatment on the germination rate and the germination vigor of the cordyceps sobolifera seeds is not obvious, the germination rate can reach over 38.89% and the germination vigor can reach over 26.67% in each treatment. However, there was some difference between treatments of germination index, where GA3The maximum germination potential was 2.49 at a concentration of 300mg/L, indicating GA3The method is not obvious for improving the germination of the iris lactea seeds.
TABLE 1 different GAs3Effect of concentration treatment on seed Germination
DIG：Date of initial germination；GR：Germination rate；GE：Germination energy；GI：Germination index.The same below.
Note: different upper case letters indicate significant difference (P < 0.01), different lower case letters indicate significant difference (P < 0.05), and n is 3, the same below.
As can be seen from Table 2, the differences of the germination rates, germination potentials and germination indexes of the rabdosia lophanthide seeds caused by the 6-BA treatment with different concentrations are not significant, the germination rates of all the treatments can reach over 95.56%, the germination potentials can reach over 47.78%, and the germination indexes can reach over 2.31, which indicates that the 6-BA does not significantly affect the germination improvement of the rabdosia lophanthide seeds.
After the iris lactea seeds are treated by 6-BA with different concentrations, the difference of the germination rate, the germination potential and the germination index between 300mg/L and other treatments is obvious or extremely obvious, and the germination rate, the germination potential and the germination index of 300mg/L are respectively 78.89%, 44.45% and 0.40, which are the maximum. Therefore, the 6-BA has obvious promotion influence on improving the iris lactea seed germination, and the optimal concentration is 300 mg/L.
The influence difference of the 6-BA treatment with different concentrations on the germination vigor of the cordyceps sobolifera seeds is not obvious, and the germination vigor of each treatment can reach more than 37.78 percent. But the influence difference on the germination rate and the germination index is extremely obvious, when the concentration is 100 mg/L-300 mg/L, the germination rate and the germination index are both extremely higher than those of a control, the germination rate reaches more than 78.89%, and the germination index reaches more than 3.50. As can be seen, the 6-BA has a very significant promoting effect on the dormancy of the broken cordyceps sobolifera seeds, the optimal concentration is 200mg/L, and the germination rate, the germination vigor and the germination index are 93.33%, 50.00% and 4.36 respectively.
TABLE 2 Effect of different concentrations of 6-BA treatment on seed Germination
As can be seen from Table 3, KNO was used at different concentrations3The difference of the treatment on the germination rate and the germination potential of the rabdosia lophanthide is not obvious, the germination rate of each treatment reaches over 90 percent, and the germination potential is not obviousAll reach more than 41.11 percent. But has obvious influence difference on germination indexes when KNO is used3At a concentration of 0, the germination index was 2.39. Thus, KNO3Has no obvious promotion effect on improving the seed germination of the rabdosia lophanthide.
Different concentrations of KNO3The treatment shortens the initial germination days of the iris lactea seeds by 6-16 days, has obvious influence difference on the germination rate, the germination vigor and the germination index, and when KNO is adopted3When the concentration is 0.1-0.4%, the germination rate, germination vigor and germination index are remarkably higher than those of a control, the germination rate is more than 86.67%, the germination vigor is more than 62.22%, the germination index is more than 0.48, and when the concentration is 0.4%, the germination rate and the germination vigor are the highest and are respectively 90.00% and 68.89%. Thus, KNO3Has extremely obvious promotion effect on improving the germination of the iris lactea seeds, and the optimal concentration is 0.4 percent.
Different concentrations of KNO3The difference of the treatment on the germination rate and the germination index of the cordyceps sobolifera is not obvious, the germination rate reaches over 37.78 percent, and the germination index reaches over 1.91. But the difference of the influence on the germination vigor is not significant, and when the concentration is 0.4%, the germination rate, the germination vigor and the germination index are respectively 55.56%, 48.89% and 2.66. Thus, KNO3Has no obvious promotion effect on improving germination of the cordyceps sobolifera seeds, and the optimal concentration is 0.4%.
TABLE 3 different KNO3Effect of concentration treatment on seed Germination
As can be seen from Table 4, the concentrations H were varied2O2The difference of the influence of the treatment on the germination rate, the germination vigour and the germination index of the rabbitia seed is not significant, the germination rate reaches over 88.89 percent, the germination vigour reaches over 30.0 percent, the germination index reaches over 2.16 percent, and when the concentration is 0.2 percent, the germination rate and the germination index are highest and are respectively 98.89 percent and 2.31 percent. It can be seen from this that H2O2Has no obvious promotion effect on improving the seed germination of the rabdosia lophanthide.
Different concentrations of H2O2The initial germination days of the iris lactea seeds are delayed by 10-16 days by treatment. When the concentration is 0.1-0.4%, the germination rate is extremely higher than that of a control, and the germination rate reaches above 85.56%. It can be seen from this that H2O2Has extremely obvious promotion function on improving the germination of the iris lactea seeds, the optimal concentration is 0.1 percent, and the germination rate, the germination vigor and the germination index are respectively 86.67 percent, 61.11 percent and 0.50 percent.
Different concentrations of H2O2The treatment shortens the initial germination days of the cordyceps sobolifera seeds by 3 days, and the influence difference on the germination rate and the germination index is obvious or extremely obvious, but the influence difference on the germination vigor of the seeds is not obvious. When the concentration is 0.4%, the germination rate, germination vigor and germination index are all the highest and are respectively 50.00%, 30.00% and 2.66%. It can be seen from this that H2O2Has obvious promotion effect on improving the germination of the cordyceps sobolifera seeds, and the optimal concentration is 0.4%.
TABLE 4 different H2O2Effect of concentration treatment on seed Germination
In summary, 6-BA, KNO3、H2O2The seed germination of the rabdosia lophanthide is not obviously promoted; 6-BA, KNO3And H2O2The Chinese iris seed germination can be obviously promoted, the effect is obvious, the optimal concentrations are respectively 300mg/L, 0.4% and 0.1%, and the germination rate, the germination vigor and the germination index are respectively 78.89%, 44.45% and 0.40; 90.00%, 68.89%, 0.49; 86.67%, 61.11%, 0.50%. 6-BA and H2O2Has extremely obvious or obvious promotion influence on improving the germination of the cordyceps sobolifera seeds, the optimal concentrations are respectively 200mg/L and 0.4 percent, and the germination rate, the germination vigor and the germination index are respectively 93.33 percent, 50.00 percent and 4.36 percent; 50.00%, 30.00%, 2.66%. But KNO3Has no obvious promotion effect on improving the germination of the cordyceps sobolifera seeds.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (4)
1. The method for improving iris seed germination is characterized in that iris seed is soaked in 6-BA with the concentration of 300mg/L for 48 hours and then sown, the seed germination temperature is 30 ℃, the seed germination temperature is 8 hours, the seed germination temperature is 20 ℃, the seed germination temperature is 16 hours, and the illumination intensity is 2400-2600 lx.
2. A method for improving iris seed germination is characterized in that iris seed is processed by using H with volume percentage concentration of 0.1%2O2Seed soaking is carried out for 24 hours, and then seeding is carried out; the seed germination temperature is 30 ℃, the illumination is carried out for 8 hours, the temperature is 20 ℃, the darkness is carried out for 16 hours, and the illumination intensity is 2400-2600 lx.
3. A method for improving iris plant seed germination is characterized in that iris plant seeds are cordyceps sobolifera seeds, and seeds are sown after being soaked in 6-BA with the concentration of 200mg/L for 48 hours; the seed germination temperature is 30 ℃, the illumination is carried out for 8 hours, the temperature is 20 ℃, the darkness is carried out for 16 hours, and the illumination intensity is 2400-2600 lx.
4. A method for improving seed germination of Iris plants is characterized in that the Iris plants are Iris pepo seeds, and the Iris plants are treated by H with the volume percentage concentration of 0.4%2O2Seed soaking is carried out for 24 hours, and then seeding is carried out; the seed germination temperature is 30 ℃, the illumination is carried out for 8 hours, the temperature is 20 ℃, the darkness is carried out for 16 hours, and the illumination intensity is 2400-2600 lx.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099422A (en) * | 2007-07-25 | 2008-01-09 | 毕慧滨 | Method for improving iris lactea seed germination rate |
| CN105638026A (en) * | 2016-01-11 | 2016-06-08 | 河北省林业科学研究院 | Method for breaking dormancy of iris barbata hybrid seeds |
| CN105684595A (en) * | 2016-01-19 | 2016-06-22 | 河北省林业科学研究院 | Method for breaking dormancy of hybrid seeds of bearded iris |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099422A (en) * | 2007-07-25 | 2008-01-09 | 毕慧滨 | Method for improving iris lactea seed germination rate |
| CN105638026A (en) * | 2016-01-11 | 2016-06-08 | 河北省林业科学研究院 | Method for breaking dormancy of iris barbata hybrid seeds |
| CN105684595A (en) * | 2016-01-19 | 2016-06-22 | 河北省林业科学研究院 | Method for breaking dormancy of hybrid seeds of bearded iris |
Non-Patent Citations (1)
| Title |
|---|
| "Stratification and priming may improve seed germination of purple coneflower，blue-flag Iris and evening primrose";David Wees 等;《ACTA HORTICULTURAE 》;20041231(第629期);摘要 * |
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