CN103766040B - A kind of method improving wild Root of Nakedstamen Monkshood seed germination rate - Google Patents
A kind of method improving wild Root of Nakedstamen Monkshood seed germination rate Download PDFInfo
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- CN103766040B CN103766040B CN201410020367.XA CN201410020367A CN103766040B CN 103766040 B CN103766040 B CN 103766040B CN 201410020367 A CN201410020367 A CN 201410020367A CN 103766040 B CN103766040 B CN 103766040B
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Abstract
The invention discloses a kind of method improving wild Root of Nakedstamen Monkshood seed germination rate, be wild Root of Nakedstamen Monkshood seed is shelled sterilization after, 24h is soaked by the Gibberellins solution of 1000mg/L concentration, respectively in illumination and dark, 18 DEG C of (12h) ~ 25 (12h) DEG C Fluctuation temperature cultures impel the method for seed germination.Beneficial effect of the present invention is: utilize plant hormone GA
3process wild Root of Nakedstamen Monkshood seed; under light illumination; Root of Nakedstamen Monkshood seed germination rate can be made to bring up to 91.7% from original 7%; 95.7% is brought up under dark condition; its advantage is: method is simple, economical, not by the impact of the factors such as season, weather, natural calamity; utilize this technology to solve Root of Nakedstamen Monkshood seminal propagation ability is low, scarcity of resources, artificial domesticating cultivation to the demand of Root of Nakedstamen Monkshood seed, to protection wild Root of Nakedstamen Monkshood natural resources and natural ecological environment significant.
Description
Technical field
The present invention relates to the method improving seed germination rate, be specifically related to a kind of method improving wild Root of Nakedstamen Monkshood seed germination rate.
Background technology
Root of Nakedstamen Monkshood (AconitumgymnandrumMaxim.) is unique species of Ranunculaceae (Ranunculaceae) delphinium grandiflorum race (tribeDelphinieae) Aconitum (AconitumL.) Root of Nakedstamen Monkshood subgenus (subgen.Gymnaconitum (Stapf) Rapaics), only be distributed in China-Himalaya floristic subregion system and neighborhood, mainly be distributed in Tibet, western Sichuan, Qinghai, SOUTH OF GANSU, be grown on the mountain region Cao Po between height above sea level 1550-3800m, meadow, limit, field and river bank sandy ground.Root of Nakedstamen Monkshood and the rare traditional Chinese medicine such as monkshood, radix aconiti agrestis affiliation close, its leaf, flower, root all can be used as medicine, and taste is pungent, warm in nature, and effect of maggot is killed in dispel the wind calmness, expelling parasite of tool, and can be used for disease such as treatment arthralgia, rheumatism etc., herb is poisonous.
But in recent years; because the medicinal of Root of Nakedstamen Monkshood and economic worth are excessively excavated or the exploitation of predation formula; it has faced resource atrophy gradually; grassland degeneration; the situation that grassland ecology balance is destroyed; therefore, the protection strengthening Wild Medicinal Plants Resources has become current important subject with reasonable and artificial domesticating cultivation.We study discovery, wild Root of Nakedstamen Monkshood seed germination rate is very low has become the primary factor limiting its procreation and cultivation, at present in wild Root of Nakedstamen Monkshood research, the overwhelming majority concentrates on the aspect such as active ingredient and pharmacological effect, and reports seldom about the research that wild Root of Nakedstamen Monkshood seed breaks in dormancy mechanism and restructuring bankrupt.Gibberellin GA
3as a Plant Hormone, there is the effect of Dormancy breaking, in other plant and seed germination research, have relevant research report, and break in dormancy mechanism and restructuring bankrupt at wild Root of Nakedstamen Monkshood and also rarely have research.
Summary of the invention
The object of the invention is for above-mentioned defect of the prior art, provide a kind of by gibberellin process, thus improve the method for wild Root of Nakedstamen Monkshood seed germination rate.
To achieve these goals, technical scheme provided by the invention is: a kind of method improving wild Root of Nakedstamen Monkshood seed germination rate, be wild Root of Nakedstamen Monkshood seed is shelled sterilization after.Root of Nakedstamen Monkshood seed 24h is soaked with gibberellin (GA3) solution that concentration is 1000mg/L, respectively under illumination and dark condition, 18 DEG C of (12h) ~ 25 (12h) DEG C Fluctuation temperature cultures to sprouting, thus improve the method for wild Root of Nakedstamen Monkshood seed germination rate.
Further, a kind of above-mentioned method improving wild Root of Nakedstamen Monkshood seed germination rate, comprises the following steps:
1) seed of wild Root of Nakedstamen Monkshood is got, seed is air-dry, manually remove shell, preserve under low temperature, dry environment, obtain the Root of Nakedstamen Monkshood seed that shells;
2) the Root of Nakedstamen Monkshood seed that step 1) obtained shelling is selected, and selects Root of Nakedstamen Monkshood seed that is in the same size, full grains, first uses liquor natrii hypochloritis's sterilization 5-10min of 5%, then with distilled water flushing 2-3 time, Root of Nakedstamen Monkshood seed after being sterilized;
3) by step 2) Root of Nakedstamen Monkshood seed soaks 24h in 1000mg/L Gibberellins solution after the sterilization that obtains, and be then placed in the culture dish being lined with two layers of filter paper, every ware places 100, is provided with three repetitions, obtains Root of Nakedstamen Monkshood seed after gibberellin process;
4) after gibberellin process step 3) obtained, Root of Nakedstamen Monkshood seed is placed in two incubators, respectively in 3000LX illumination and dark, cultivates under 18 DEG C of (12h) ~ 25 (12h) DEG C temperature match curing conditions, can obtain sprouting Root of Nakedstamen Monkshood germination seed.
Second object of the present invention there is provided gibberellin and is improving the application in wild Root of Nakedstamen Monkshood seed germination rate.
Beneficial effect of the present invention is: the present invention utilizes plant hormone GA
3process the method breaking dormancy of wild Root of Nakedstamen Monkshood seed; its advantage is: under light illumination; Root of Nakedstamen Monkshood seed germination rate can be made to bring up to 91.7% from original 7%; 95.7% is brought up under dark condition; method is simple; economical; not by the impact of the factors such as season, weather, natural calamity; Root of Nakedstamen Monkshood seminal propagation ability is low, the kind Geological Problems of scarcity of resources, artificial domesticating cultivation to utilize this technology to solve, and to preserving the ecological environment, protecting, kind of the aspect such as matter and artificial cultivation is significant.
Embodiment
Embodiment 1:
Improve a method for wild Root of Nakedstamen Monkshood seed germination rate, comprise the following steps:
1, test material is wild Root of Nakedstamen Monkshood seed, adopts the Zhua Xi grassland, Tianzhu, Gansu Province in height above sea level 2960-2970m, the plant of Root of Nakedstamen Monkshood directly gathers, after seed is air-dry, manually remove shell, save backup under low temperature, dry environment;
2, select in the same size, full Root of Nakedstamen Monkshood seed, first use liquor natrii hypochloritis's sterilization 5min of 5%, then use distilled water flushing 2 times, by Root of Nakedstamen Monkshood seed at 1000mg/LGA
3soak 24h in solution, be then placed in the culture dish (φ 9cm) being lined with two layers of filter paper, every ware places 100, and each concentration establishes three repetitions;
3, all culture dishes are placed in two incubators, respectively in 3000LX illumination and dark, cultivate under 18 DEG C of (12h) ~ 25 (12h) DEG C temperature match curing conditions, show money or valuables one carries unintentionally as sprouting mark using seed, every day adds up germination rate, add up 8d altogether, 5d measures germination vigor, and 8d measures germination rate, germination index and vitality index;
4, seed germination mensuration formula is as follows:
Germination rate (%)=(subnumber is planted experimentally in chitting piece number/confession) × 100%;
Germination vigor (%)=(seed grain number/confession chitting piece grain number × 100% germinateed in 5d);
Germination index (Gi)=∑ Gt/Dt; Wherein Gt is the germinative number of 8d, and Dt is for germinateing day;
Vitality index (VI)=germination index × seedling mean fresh (g).
5, GA
3process pole significantly promotes the sprouting (P ﹤ 0.01) of Root of Nakedstamen Monkshood seed, and table 1 and 2 displays, be only respectively 7% and 24.3% to the germination rate impinged upon under illumination and dark condition, at variable concentrations GA
3in process, improve the most remarkable with 1000mg/L germination rate, GA
3under during concentration 1000mg/L+ illumination, germination rate can bring up to 91.7%, 1000mg/L+ dark condition, germination rate reaches 95.7%, through the GA of 1000mg/L
3its germination vigor of Root of Nakedstamen Monkshood seed, the germination index of process are all significantly higher than contrast (P ﹤ 0.01) in pole, under illumination condition, exceed respectively than contrast: 48.7%, 16.3%; Under dark condition, exceed respectively: 74.3%, 17.6%.Wherein, table 1 is variable concentrations GA
3solution is on the impact of Root of Nakedstamen Monkshood seed germination rate under illumination condition, and table 2 is that variable concentrations GA3 solution is on the impact of Root of Nakedstamen Monkshood seed germination rate under dark condition.
Table 1
。
Table 2
。
Note: the different capitalization of same column represents that difference extremely significantly (P ﹤ 0.01), and different lowercase alphabet shows significant difference (P ﹤ 0.05).
Embodiment 2:
Embodiment is identical with embodiment 1, and institute's difference is, in step 2, with liquor natrii hypochloritis's sterilization 10min of 5%, then uses distilled water flushing 3 times.
Embodiment 3:
Embodiment is identical with embodiment 1, and institute's difference is, in step 2, with liquor natrii hypochloritis's sterilization 8min of 5%, then uses distilled water flushing 3 times.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (1)
1. improve a method for wild Root of Nakedstamen Monkshood seed germination rate, it is characterized in that, comprise the following steps:
1) seed of wild Root of Nakedstamen Monkshood is got, seed is air-dry, manually remove shell, preserve under low temperature, dry environment, obtain the Root of Nakedstamen Monkshood seed that shells;
2) the Root of Nakedstamen Monkshood seed that step 1) obtained shelling is selected, and selects Root of Nakedstamen Monkshood seed that is in the same size, full grains, first uses liquor natrii hypochloritis's sterilization 5-10min of 5%, then with distilled water flushing 2-3 time, Root of Nakedstamen Monkshood seed after being sterilized;
3) by step 2) Root of Nakedstamen Monkshood seed soaks 24h in 1000mg/L Gibberellins solution after the sterilization that obtains, and be then placed in the culture dish being lined with two layers of filter paper, every ware places 100, is provided with three repetitions, obtains Root of Nakedstamen Monkshood seed after gibberellin process;
4) after gibberellin process step 3) obtained, Root of Nakedstamen Monkshood seed is placed in two illumination boxs and cultivates, respectively in 3000LX illumination and dark, temperature 18 DEG C is cultivated under keeping 12h and temperature 25 DEG C to keep the alternately temperature match curing conditions of 12h, can obtain sprouting Root of Nakedstamen Monkshood germination seed.
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| CN104541665B (en) * | 2015-01-23 | 2017-04-12 | 中国农业科学院特产研究所 | Method for breaking dormancy of aconitum kusnezoffii reichb seeds |
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| CN101099422A (en) * | 2007-07-25 | 2008-01-09 | 毕慧滨 | Method for improving iris lactea seed germination rate |
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| CN101099422A (en) * | 2007-07-25 | 2008-01-09 | 毕慧滨 | Method for improving iris lactea seed germination rate |
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| Title |
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| 周丽霞."乌头属部分植物的资源调查及引种栽培研究".《中国优秀硕士学位论文全文数据库》.2009,(第1期),第26页第3.1.2.4、3.1.2.5节. * |
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