CN101530026B - Method for improving rate of emergence of Yunnan gentian seed - Google Patents
Method for improving rate of emergence of Yunnan gentian seed Download PDFInfo
- Publication number
- CN101530026B CN101530026B CN2009100941771A CN200910094177A CN101530026B CN 101530026 B CN101530026 B CN 101530026B CN 2009100941771 A CN2009100941771 A CN 2009100941771A CN 200910094177 A CN200910094177 A CN 200910094177A CN 101530026 B CN101530026 B CN 101530026B
- Authority
- CN
- China
- Prior art keywords
- seed
- fruit
- yunnan
- ovary
- emergence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a method for improving rate of emergence of Yunnan gentian seed, belonging to the technical field of plant cultivation. The method comprises the following concrete steps: a. collecting Yunnan gentian fruit with cracked or uncracked ovary extending out of withered corolla and seed coat becoming hardened in the late January of the current year; burying the Yunnan gentian fruit which is not fully mature with the ovary not extending out of the withered corolla and the seed being green, the seed skin being not hardened in dry sand for a month at the temperature of 0-5 DEG C after the fruit is collected and waiting for the fruit to become mature; b. taking the seed out of the green fruit and cleaning the seed; c. placing the washed seed in gibberellin with a concentration of 50-1000mg/L and soaking the seed for 24 hours under the condition of natural lighting at the temperature of 25 DEG C for facilitating the seed to germinate. The method of the invention has the advantages of simple operation, low cost, easy promotion and a rate of emergence over 84%, thus laying a good foundation for artificial propagation of the Yunnan gentian and enjoying very good social and economic benefits.
Description
Technical field:
The present invention relates to a kind of method that improves rate of emergence of Yunnan gentian seed seed, platymiscium culture technique field.
Background technology:
Yunnan rough gentian (Gentiana rigescens) has another name called hard rough gentian, is Gentianaceae, Gentiana herbaceos perennial.Originate in Yunnan, Sichuan, Guizhou, Hunan, Guangxi.Be born in patana, the shrubbery, in sylvan life and the mountain valley, height above sea level 1100-3000 rice.Rhizome and root are used as medicine, and are one of former plant of Chinese medicine rough gentian, for large traditional Chinese medicine commonly used, find a good sale in domestic each department, have effects such as heat-clearing and damp-drying drug, purging the liver of pathogenic fire courage fire.
Yunnan rough gentian rhizome is extremely short, and root system is undeveloped, and natural crude drugs are difficult to meet the need of market.Long-term unordered excavating also makes resource be on the verge of exhaustion.The endogenous inhibiting substances that contains certain activity in the rough gentian seed of Yunnan, sprouting has obvious inhibitory action to seed self, under normal circumstances difficult the germination, survival rate is low, brings very big difficulty for planting experiment and demonstration work.
Through the document retrieval, do not see the public reported identical with the present invention.
Summary of the invention:
The object of the present invention is to provide a kind of germinating method that improves Yunnan rough gentian seed, for good basis is established in the artificial propagation of Yunnan rough gentian.
The object of the invention is achieved through following technical proposals:
A. gather ovary in late January then and from withered corolla, stretch out, ovary cracking or cracking are as yet planted the Yunnan rough gentian fruit of skin hardening; Ovary is not stretched out from withered corolla as yet, and seed be green, plants the skin not full ripe fruit of hardening not as yet, and collection was buried one month in 0-5 ℃ of dried sand after returning, and treated its maturation;
B. seed is taken out from mellow fruit, clean;
C. with the seed of cleaning put into the gibberellin of 50-1000mg/L concentration, after soaking 24 hours under 25 ℃ of natural lighting conditions, carry out seed sprouting.
The present invention has simple to operate, and cost is low, is easy to promote, and percentage of seedgermination is established good basis up to the advantage more than 84% for the artificial propagation of Yunnan rough gentian, has good economic benefit and social benefit.
Embodiment:
1, confirming of picking time:
The rough gentian seed maturity is taked the fruit of waiter township, Lincang, Yunnan differing maturity respectively in Yunnan; Randomly draw 100 seeds and repeat indoor germination naturally for three times; Place and measure percentage of seedgermination after 30 days, measure the result and show: late January is the reasonable picking time of Yunnan rough gentian seed then.
2, measure percentage of seedgermination:
I handles ovary and from withered corolla, does not stretch out as yet, and seed be green, plants skin not hardening as yet, is divided into promptly sowing, adopting the back after adopting and place and did sprouting test after the drying in 30 days, detects once mensuration percentage of seedgermination and germination vigor in per 24 hours;
II handles ovary and from withered corolla, stretches out, ovary cracking or cracking as yet, and the hardening of seed kind skin is divided into promptly sowing, placing after adopting and did sprouting test after the drying in 30 days, detects once in per 24 hours, measures percentage of seedgermination and germination vigor.
The relation of table 1 kernel maturing and seed germination rate
Can find out by table 1, i.e. sowing after immature seed is gathered, germination rate is extremely low, but after placing January, germination rate rises to 52.33%; The germination rate of promptly sowing after mature seed is gathered is 66.00%, has descended 6 percentage points and place the germination rate of sowing again January after adopting.
3, seed treatment
A. seed soaking disinfection
Gibberellin concentration is set at A (CK), B 50mg/L, and C 100mg/L, D 150mg/L, E 200mg/L, F 300mg/L, G 400mg/L, H 500mg/L, I 1000mg/L places Gibberellins solution to soak sterilization in 24 hours in seed.The result is as shown in table 2.
Table 2 gibberellin concentration is to the influence of percentage of seedgermination
Can find out that by table 2 gibberellin in the 50-1000mg/L concentration range has obvious facilitation to seed sprouting, and Yunnan rough gentian seed germination rate is brought up to more than 84%.
B. illumination condition confirms
Gather mature seed then, establish 2 processing of illumination and dark (covering culture dish with dark cover) behind the soaking disinfection, place 25 ℃ constant temperature illumination box, add up its percentage of seedgermination and germination vigor, the result is as shown in table 3.
Table 3 illumination is to the influence of seed germination
Can find out by table 3, Yunnan rough gentian seed germination rate under dark condition extremely low (6.33%), deficiency has 1/10 of illumination percentage of seedgermination, explains that 25 ℃ constant temperature illumination helps Yunnan rough gentian seed germination.
Seed carries out seed sprouting after soaking 24 hours under 25 ℃ of natural lighting conditions.
Claims (1)
1. method that improves rate of emergence of Yunnan gentian seed seed is characterized in that steps of the method are:
A. gather ovary in late January then and from withered corolla, stretch out, ovary cracking or cracking are as yet planted the Yunnan rough gentian fruit of skin hardening; Ovary is not stretched out from withered corolla as yet, and seed be green, plants the skin not full ripe fruit of hardening not as yet, and collection was buried one month in 0-5 ℃ of dried sand after returning, and treated its maturation;
B. seed is taken out from mellow fruit, clean;
C. with the seed of cleaning put into the gibberellin of 50-1000mg/L concentration, after soaking 24 hours under 25 ℃ of natural lighting conditions, carry out seed sprouting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100941771A CN101530026B (en) | 2009-03-10 | 2009-03-10 | Method for improving rate of emergence of Yunnan gentian seed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100941771A CN101530026B (en) | 2009-03-10 | 2009-03-10 | Method for improving rate of emergence of Yunnan gentian seed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101530026A CN101530026A (en) | 2009-09-16 |
| CN101530026B true CN101530026B (en) | 2012-02-15 |
Family
ID=41101042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100941771A Expired - Fee Related CN101530026B (en) | 2009-03-10 | 2009-03-10 | Method for improving rate of emergence of Yunnan gentian seed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101530026B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102626023B (en) * | 2012-04-01 | 2014-02-12 | 广西壮族自治区药用植物园 | Gentiana loureiri introduction and cultivation method and substrate |
| CN105532245A (en) * | 2016-01-14 | 2016-05-04 | 六枝特区道地种养殖农民专业合作社 | Seedling culture method for gentiana rigescens |
| CN106717260B (en) * | 2016-11-29 | 2021-01-08 | 青岛松良基因科技有限公司 | Gentiana scabra seedling growing method and Gentiana scabra planting method |
| CN115612708B (en) * | 2022-12-19 | 2023-06-27 | 云南省农业科学院药用植物研究所 | Method for producing gentiopicroside by utilizing gentiana rigescens calli |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1047499C (en) * | 1993-11-02 | 1999-12-22 | 杨友仁 | Man-controlled hastening germination liquid state sowing method for rough gentian |
-
2009
- 2009-03-10 CN CN2009100941771A patent/CN101530026B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1047499C (en) * | 1993-11-02 | 1999-12-22 | 杨友仁 | Man-controlled hastening germination liquid state sowing method for rough gentian |
Non-Patent Citations (3)
| Title |
|---|
| 关家声.赤霉素处理龙胆种子发芽率高.《自然资源研究》.1987,(第1期),26-28. * |
| 申志英等.提高龙胆种子发芽率试验.《中药材》.2004,第27卷(第11期),801-802. * |
| 赵敏.龙胆种子萌发与水溶性内源抑制物质.《植物生理学通讯》.2004,第40卷(第6期),677-679. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101530026A (en) | 2009-09-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102870564B (en) | Fast efficient propagation method of Paris polyphylla | |
| CN103609223B (en) | Gibberellin and wet sand bed amass the method that paris polyphylla seed quick-speed germination is bred | |
| CN104380942B (en) | A kind of processing method making long of Rhizoma Paridis seed four months, coming into leaves for eight months | |
| CN104737665A (en) | Method for germinating polygonatum kingianum seeds | |
| Li et al. | Responses of plant development, biomass and seed production of direct sown oilseed rape (Brassica napus) to nitrogen application at different stages in Yangtze River Basin | |
| CN107258294A (en) | A kind of breeding method of Yunnan Paris polyphylla seed | |
| CN105900771A (en) | Cyclocarya paliurus propagating method | |
| CN101530026B (en) | Method for improving rate of emergence of Yunnan gentian seed | |
| CN105409514A (en) | Fast seedling culture method of paris polyphylla | |
| CN106171504A (en) | A kind of Radix Scutellariae artificial cultivation method | |
| Dyksterhuis | Axillary cleistogenes in Stipa leucotricha and their role in nature | |
| CN100594773C (en) | Method for treating spring sowing seeds of bulbus fritillariae cirrhosae | |
| Rhie et al. | Seed dormancy and germination of Epimedium koreanum Nakai | |
| CN102972118A (en) | Magnolia biloba seed treatment method | |
| CN103340046A (en) | Method for promoting halogeton sativus seeds to rapidly germinate | |
| CN104429224B (en) | One is broken the seed dormant method of radix tetrastigme | |
| CN103444415B (en) | Breeding seedling-growing method for polygonum capitatum of Relinqing granule medicine materials | |
| CN102577698A (en) | Treatment method of white flower loropetalum chinense seeds | |
| CN102197775A (en) | Method for culturing cephalataxus fortunei | |
| CN102090179B (en) | Method for processing aconitum seeds | |
| CN103766040B (en) | A kind of method improving wild Root of Nakedstamen Monkshood seed germination rate | |
| CN103843638A (en) | Method for increasing afforestation survival rate of bare-rooted seedlings of apocaryas | |
| CN110140472B (en) | Method for promoting germination of paris polyphylla seeds | |
| CN103843545A (en) | Method for domesticating and cultivating high-quality rootstock seedlings by utilizing wild eggplant seeds | |
| Na et al. | Changes of seed quality of Chinese milk vetch (Astragalus sinicus L.) during seed developmental stages |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120215 Termination date: 20140310 |