CN104737665A - Method for germinating polygonatum kingianum seeds - Google Patents
Method for germinating polygonatum kingianum seeds Download PDFInfo
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- CN104737665A CN104737665A CN201510097497.8A CN201510097497A CN104737665A CN 104737665 A CN104737665 A CN 104737665A CN 201510097497 A CN201510097497 A CN 201510097497A CN 104737665 A CN104737665 A CN 104737665A
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Abstract
The invention discloses a method for germinating polygonatum kingianum seeds, and belongs to the technical field of planting of traditional Chinese medicinal materials. The method comprises the following steps that (1) the polygonatum kingianum seeds are cleaned and peeled; (2) in a step of performing sand-storage treatment of a plastic basket, the plastic basket with grids is adopted, a layer of screen is cushioned at the bottom of the plastic basket, 5cm fine sands are placed on the screen, the polygonatum kingianum seeds are put on the sands and the 5cm sands cover the seeds, and such a process is repeatedly carried out for three times, namely, four layers of sands cover the three layers of polygonatum kingianum seeds at intervals; (3) in a greenhouse culture step, the plastic basket is subjected to sand storage treatment and put into a solar greenhouse, and the polygonatum kingianum seeds in plastic basket are cultured for 6 months by using a temperature and humidity control instrument under a condition that the day and night temperature in the greenhouse is kept at 30-5 DEG C and the indoor air relative humidity everyday is 40-80 percent. The method is easy, feasible, and low in cost; the germination period of the polygonatum kingianum is shortened from 2 years into 6 months, and the germination rate is increased from 30-50 percent to 90-95 percent. The method provides technical guarantee for reproduction and scale planting of the polygonatum kingianum.
Description
Technical field
The invention belongs to traditional Chinese medicine planting technology field, particularly a kind of method of P. kingianum seed germination.
Background technology
P. kingianum (Polygonatum kingianum Coll.et Hemsl.) is conventional Chinese medicine, has the medication history of more than 2000 year in China.Nature and flavor are sweet flat, enter lung, spleen, kidney channel.Effect tonifying middle-Jiao and Qi, profit cardiopulmonary, strengthening the bones and muscles, controls deficient fever and chills, and tuberculosis is spat blood, and body void food is few after being ill, and muscles and bones is weak, rheumatalgia pain, and wind favus disease is the product of tonifying yin; Pharmacological research shows that sealwort also has antibacterial action except above-mentioned effect in recent years, especially has significant effect to tubercle bacillus and fungi, has again the effect reduced blood pressure simultaneously; Recent research result also shows that sealwort is to increase coronary blood flow, alleviates atherosclerosis, and improving superoxide dismutase from liver (SOD) activity has obvious effect.For a long time, because artificial cultivation amount is less, P. kingianum crude drug source is mainly based on wild collection.Along with the increase of market to medicinal material P. kingianum demand and the sharply minimizing of wild resource, gather wild P. kingianum and not only can not meet the need of market, more destroy ecotope and P. kingianum wild resource.Due to P. kingianum seed, germination rate is lower under field conditions (factors), and under natural conditions, P. kingianum seed just can grow a slice true leaf after will experiencing 2 winter dormancies, and in P. kingianum artificial cultivation, the stem tuber that adopts carries out vegetative propagation more at present.Long-term vegetative propagation causes P. kingianum quality deterioration, and damage by disease and insect problem is day by day serious.
Document " different plant growth regulator break David's-harp seed dormancy test ", Zhang Wangfan is published in " traditional Chinese medicine journal " 06 phase in 2008, is specially: by the David's-harp seed adopted back, adopt respectively 6-BA300,200,100,50PPm; GA3:400,200,100,50PPm tetra-concentration immersion treatment 12h, 24h, clear water compares.Result of study shows to break seed dormancy, with GA
324h is best in 200PPm process, and germination rate reaches 70%; Break bud dormancy best with 6-BA200PPm process 12h, emergence rate can reach 90%.
Document " different Sand culture is on the impact of reserve substance and sprouting in sealwort seed ", Wang Zhanhong etc. are published in " seed " 02 phase in 2012, are specially: germination rate, the germination vigor of the change of soluble protein, fat, starch and soluble sugar content and seed in seed before measuring husky Tibetan and after different Sand culture.Result of the test shows: the impact of different Sand culture on reserve substance content each in sealwort seed all reaches significance level, show as soluble protein and soluble sugar content increase, fat and content of starch reduce, reserve substance constantly katabolism in the process of sand Tibetan is described, the sprouting for sealwort seed is done matter and energy and is prepared.Draw in 4 kinds of process by germination test, husky Tibetan effect is it is preferred that No. 2 (going to the outdoor husky Tibetan of pericarp) after gathering, next is No. 3 (outdoor husky Tibetan of band pericarp shifts to an earlier date rinsing peeling), and the poorest is No. 4 (fermentation peeling refrigerator sand Tibetan).Tentatively think, Sha Zangke breaks sealwort seed dormancy, and adopts the outdoor husky effect of hiding of peeling best.
Document " medicinal crop sealwort seminal propagation technical research ", Zhao to cause etc. and is published in " seed " the 24th volume 03 phase in 2005, be specially: have studied fermentation rinsing and rub rinsing sealwort fruit to the impact of seed important character, Different Harvesting Time sealwort fruit fermentation rinsing obtains the thousand kernel weight of seed and germination characteristic and seed grading, and different storage method is on the impact of sealwort Germination Characteristics.Result shows: sealwort (P.sibiricum Red.) the fruit harvesting time is generally in mid-November, David's-harp (P.cyrtonema Hua) and P. kingianum (P.kingianumColl.et Hemsl.) picking time are in late October, recommend to adopt fermentation fulling process sealwort fruit to obtain seed, recommendation adopts sand at low temperature to hide and freezing sand hides preservation seed.
Application number is: 201410130683.2, the Chinese patent that denomination of invention is " a kind of quick propagating technology method of sealwort " discloses a kind of method of sealwort Fast-propagation, be specially: adopt intermittent warming to add HORMONE TREATMENT and break seed dormancy, and utilize the seed of breaking dormancy to carry out in-vitro inducing cultivation callus as material, cultivate and root induction through differentiation, vegetative propagation sealwort seedling.Adopt this technology can greatly improve sealwort breeding rate, shorten the production cycle, reduce sowing quantity, reduce production cost.
Above-mentioned patented technology, employs ethanol, gibberellin, GA
3deng compound, not only add production cost, may chemical residue be there is, and be unfavorable for P. kingianum standardized cultivation.
By literature search, the open report identical with the present invention is had no.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, there is provided a kind of method of new P. kingianum seed germination, the method can break the P. kingianum seed dormancy phase, shortens the budding period of P. kingianum seed, accelerate the sprouting of P. kingianum seed, improve the germination rate of P. kingianum seed.
Object of the present invention is achieved through the following technical solutions:
A method for P. kingianum seed germination, comprises the following steps:
(1) P. kingianum wash seeds decortication: ripe P. kingianum green seed of gathering 9 ~ November, puts shady and cool place's after-ripening 10 ~ 15 days, then puts into water and wash pericarp by rubbing with the hands, and impurity is removed in rinsing, takes out the seed of sinking, dries moisture for subsequent use;
(2) plastic crate Sand culture: adopt the plastic crate having grid, pad one deck screen cloth, screen cloth is placed the thick river sand of 5cm bottom basket, river sand is placed the P. kingianum seed of ground floor through step (1) cleaning decortication, seed covers the thick river sand of 5cm; Then place the Yunnan yellow race of the second layer through step (1) cleaning decortication, then cover the thick river sand of 5cm; Place the Yunnan yellow race of third layer through step (1) cleaning decortication afterwards again, finally cover the thick river sand of 5cm;
(3) hot-house culture: by the plastic crate through Sand culture, adopt temperature and humidity control instrument keep diurnal temperature 30 DEG C ~ 5 DEG C in greenhouse, every day indoor air relative humidity 40 ~ 80% condition under cultivate 6 months.
Preferably:
Adopting in step (2) plastic crate Sand culture has the specification of the plastic crate of grid to be: and 35cm length × 27cm is wide × and 20cm is high, and the specification of one deck screen cloth padded bottom basket is 100 orders.
In step (2), the placement density of every layer of P. kingianum seed is preferably 20 ~ 30/cm
2.
Build one deck white film in top of greenhouse in step (3) hot-house culture, then in white film, take the black sunshade net that one deck shading rate is 50%.
Compared with prior art, innovative point of the present invention and beneficial effect are:
The present invention is simple and easy to do, with low cost, and the budding period of P. kingianum is shortened to 4 ~ 5 months from two winters of naturally sprouting, and germination rate brings up to 90 ~ 95% from naturally sprout 10 ~ 20%.Present invention employs modern installations condition and solve P. kingianum seed germination rate, problem that germination rate is lower under field conditions (factors).The present invention is that the breeding of P. kingianum and large-scale planting provide technical guarantee.
Embodiment
Embodiment 1
The present embodiment is in the Yunnan Province Agriculture Academy of Science Medicine Plant Institute laboratory implementation of Kunming, Yunnan Province.
(1) P. kingianum wash seeds decortication: ripe P. kingianum yellow green seed of gathering October, puts shady and cool place's after-ripening 15 days, then puts into water and wash pericarp by rubbing with the hands, and impurity is removed in rinsing, takes out the clean seed of sinking, dries moisture for subsequent use.
(2) plastic crate Sand culture: having the frame of plastic of grid, frame of plastic be 35cm length × 27cm wide × 20cm is high, padding one deck specification bottom frame of plastic is 100 object screen clothes, screen cloth is placed the thick river sand of 5cm, river sand is placed the P. kingianum seed of ground floor through step (1) cleaning decortication, seed covers the thick river sand of 5cm; Then place the Yunnan yellow race of the second layer through step (1) cleaning decortication, then cover the thick river sand of 5cm; Place the Yunnan yellow race of third layer through step (1) cleaning decortication afterwards again, finally cover the thick river sand of 55cm; The placement density of every layer of P. kingianum seed is 30/cm
2.
(3) hot-house culture: by the plastic crate through Sand culture, put into greenhouse, outside greenhouse, one deck white film is built at top, then in white film, take the black sunshade net that one deck shading rate is 50%.Adopt temperature and humidity control instrument keep diurnal temperature 30 DEG C ~ 5 DEG C in greenhouse, every day indoor air relative humidity 40 ~ 80% condition under cultivate 6 months.
When being cultured to mid-December, the present invention adopts plastic crate Sand culture method to occur rudiment or bud point in conjunction with the P. kingianum seed more than 90% of hot-house culture condition; After P. kingianum seed germination, be transplanted to seedbed management in time.
The present embodiment shows that the inventive method is simple and easy to do, with low cost, and the seed germination time is short, only needs 6 months, and P. kingianum seed germination rate is apparently higher than traditional earth culture method (2 winters), and seed germination rate reaches more than 90%.The method is used in the cultivation of P. kingianum, the dark welcome by P. kingianum plantation family.
Claims (4)
1. a method for P. kingianum seed germination, is characterized in that step is as follows:
(1) P. kingianum wash seeds decortication: ripe P. kingianum green seed of gathering 9 ~ November, puts shady and cool place's after-ripening 10 ~ 15 days, then puts into water and wash pericarp by rubbing with the hands, and impurity is removed in rinsing, takes out the seed of sinking, dries moisture for subsequent use;
(2) plastic crate Sand culture: adopt the plastic crate having grid, basket bottom pad one deck screen cloth, screen cloth is placed the thick river sand of 5cm, river sand is placed the P. kingianum seed of ground floor through step (1) cleaning decortication, seed covers the thick river sand of 5cm, then place the Yunnan yellow race of the second layer through step (1) cleaning decortication, then cover the thick river sand of 5cm, place the Yunnan yellow race of third layer through step (1) cleaning decortication afterwards again, finally cover the thick river sand of 55cm;
(3) hot-house culture: by the plastic crate through Sand culture, put into greenhouse, adopt temperature and humidity control instrument keep diurnal temperature 30 DEG C ~ 5 DEG C in greenhouse, every day indoor air relative humidity 40 ~ 80% condition under cultivate 6 months.
2. the method for a kind of P. kingianum seed germination according to claim 1, is characterized in that: in step (2), the placement density of every layer of P. kingianum seed is 20 ~ 30/cm
2.
3. the method for a kind of P. kingianum seed germination according to claim 1 and 2, it is characterized in that: in step (3) hot-house culture, build one deck white film in top of greenhouse, then in white film, take the black sunshade net that one deck shading rate is 50%.
4. the method for a kind of P. kingianum seed germination according to claim 1, it is characterized in that: adopting in step (2) plastic crate Sand culture has the specification of the plastic crate of grid to be: 35cm length × 27cm is wide × and 20cm is high, and the specification of one deck screen cloth padded bottom basket is 100 orders.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920198A (en) * | 2015-07-14 | 2015-09-23 | 安徽省应用技术研究院 | Seed germination acceleration seedling culturing method for polygonatum cyrtonema |
| CN105165321A (en) * | 2015-08-11 | 2015-12-23 | 玉龙县凤翔旺生态养殖有限责任公司 | Polygonatum kingianum cultivation method |
| CN105284410A (en) * | 2015-12-01 | 2016-02-03 | 普洱南药庄园科技有限公司 | Polygonatum kingianum tuber propagating and growing method |
| CN106922248A (en) * | 2017-03-24 | 2017-07-07 | 红河道地中药材开发有限公司 | The processing method of P. kingianum seed |
| CN107155591A (en) * | 2017-05-18 | 2017-09-15 | 新化县绿源农林科技有限公司 | A kind of mating system of David's-harp seed |
| CN109548426A (en) * | 2019-01-18 | 2019-04-02 | 杭州职业技术学院 | A kind of the presprouting of seeds method for culturing seedlings and device of polygonatum cyrtonema |
| CN110012804A (en) * | 2017-11-08 | 2019-07-16 | 马永升 | Rhizoma polygonati hayashishita semi-wild efficiency cultivation technology for high |
| CN110402637A (en) * | 2019-05-20 | 2019-11-05 | 重庆春京药业有限责任公司 | A kind of germination accelerating method of rhizoma polygonati seed |
| CN110521325A (en) * | 2019-09-04 | 2019-12-03 | 昆明坤地农业开发有限公司 | A kind of method of polygonatum kingianurn seed fast seedling growing |
| CN111543271A (en) * | 2020-06-08 | 2020-08-18 | 普洱淞茂谷林下中药材开发有限公司 | Polygonatum sibiricum seedling breeding method |
| CN112514594A (en) * | 2020-10-27 | 2021-03-19 | 云南省热带作物科学研究所 | Method for efficiently screening macadimia nut germinating seeds |
| CN114830867A (en) * | 2022-04-26 | 2022-08-02 | 云南省农业科学院药用植物研究所 | Method for improving germination rate of polygonatum kingianum seeds |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920198A (en) * | 2015-07-14 | 2015-09-23 | 安徽省应用技术研究院 | Seed germination acceleration seedling culturing method for polygonatum cyrtonema |
| CN105165321A (en) * | 2015-08-11 | 2015-12-23 | 玉龙县凤翔旺生态养殖有限责任公司 | Polygonatum kingianum cultivation method |
| CN105284410A (en) * | 2015-12-01 | 2016-02-03 | 普洱南药庄园科技有限公司 | Polygonatum kingianum tuber propagating and growing method |
| CN106922248A (en) * | 2017-03-24 | 2017-07-07 | 红河道地中药材开发有限公司 | The processing method of P. kingianum seed |
| CN107155591A (en) * | 2017-05-18 | 2017-09-15 | 新化县绿源农林科技有限公司 | A kind of mating system of David's-harp seed |
| CN110012804A (en) * | 2017-11-08 | 2019-07-16 | 马永升 | Rhizoma polygonati hayashishita semi-wild efficiency cultivation technology for high |
| CN109548426A (en) * | 2019-01-18 | 2019-04-02 | 杭州职业技术学院 | A kind of the presprouting of seeds method for culturing seedlings and device of polygonatum cyrtonema |
| CN110402637A (en) * | 2019-05-20 | 2019-11-05 | 重庆春京药业有限责任公司 | A kind of germination accelerating method of rhizoma polygonati seed |
| CN110521325A (en) * | 2019-09-04 | 2019-12-03 | 昆明坤地农业开发有限公司 | A kind of method of polygonatum kingianurn seed fast seedling growing |
| CN111543271A (en) * | 2020-06-08 | 2020-08-18 | 普洱淞茂谷林下中药材开发有限公司 | Polygonatum sibiricum seedling breeding method |
| CN112514594A (en) * | 2020-10-27 | 2021-03-19 | 云南省热带作物科学研究所 | Method for efficiently screening macadimia nut germinating seeds |
| CN114830867A (en) * | 2022-04-26 | 2022-08-02 | 云南省农业科学院药用植物研究所 | Method for improving germination rate of polygonatum kingianum seeds |
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Application publication date: 20150701 |