CN110402637A - A kind of germination accelerating method of rhizoma polygonati seed - Google Patents
A kind of germination accelerating method of rhizoma polygonati seed Download PDFInfo
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- CN110402637A CN110402637A CN201910419345.3A CN201910419345A CN110402637A CN 110402637 A CN110402637 A CN 110402637A CN 201910419345 A CN201910419345 A CN 201910419345A CN 110402637 A CN110402637 A CN 110402637A
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- seed
- rhizoma polygonati
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- lamination
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- Soil Sciences (AREA)
- Environmental Sciences (AREA)
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Abstract
The present invention relates to rhizoma polygonati presprouting of seeds technical fields, it is specific to disclose a kind of germination accelerating method of rhizoma polygonati seed, there is hibernation feature for rhizoma polygonati seed, it is sprouted naturally needs could emerge within 15 months, and the shortcomings such as emergence rate is only 46.2%, and seedling-growing time is different, and uniformity is low, there is provided one kind can make rhizoma polygonati seed tidy seedlings output, emergence rate in 5-6 months can reach 80% Stratification technique method.To effectively facilitate the germination of rhizoma polygonati seed, the germination percentage and uniformity of rhizoma polygonati seed are improved.
Description
Technical field
The present invention relates to the technical methods of rhizoma polygonati presprouting of seeds to promote rhizoma polygonati more particularly to rhizoma polygonati seed dormancy is broken
Germination improves the technical method of germination percentage and uniformity.
Background technique
Seed dormancy refers to that the great-hearted seed of tool is in suitable sprouting condition and cannot normally sprout.It is plant hair
A break-off phenomenon during educating, or being known as " life hidden " phenomenon is both one critically important for plant itself
Vital movement process, and be a kind of beneficial biological characteristics, it is a kind of pair of environment item that plant obtains by long-term evolution
The biological adaptation of part and seasonal variety.
Seed dormancy is the long-term acclimatization variation of plant as a result, can sprout to avoid in unsuitable season, with
" resting seed " this form escapes the severe environmental conditions such as severe cold, heat, arid, to the life for maintaining plant, produces offspring
There is profound significance.Seed dormancy can also cause certain difficulty to introducing and planting, have the seed of suspend mode to need to take some arrange
Breaking dormancy is applied, this is just to begin sowing in good time to bring some extra works.Many traditional Chinese medicinal seeds dormant periods are up to 0.5~2 year, have
Wild characters weight traditional Chinese medicinal seeds dormant period it is different in size, germinate it is extremely irregular, this just causes certain difficulty to introducing and planting.
Therefore, how to break rhizoma polygonati seed dormancy, promote rhizoma polygonati germination, improve germination percentage and uniformity is current
One technical problem.
Summary of the invention
The technical problems to be solved by the present invention are: how to break rhizoma polygonati seed dormancy, promote rhizoma polygonati germination, improves
Germination percentage and uniformity.
Technical solution:
The present invention provides the germination accelerating method of one seed of kind of rhizoma polygonati, the germination accelerating method includes: 1) to carry out lamination to seed
Processing, Stratificated treatment condition include: that lamination sand water content is 70%, and lamination temperature is 10 ± 1 DEG C, lamination duration are as follows: 0-30 days；
2) take out seed, go it is husky clean, soaked seed 2 hours with the GA3 of 100mg/L, then place 30 in 20 ± 1 DEG C of growth chambers~
90 days；3) seed is taken out, sand is gone to clean, is soaked seed 2 hours with the IAA of concentration 25mg/L, then places 20 ± 1 DEG C of growth chambers
In 90~150 days.
This hair name is 0,30,60,90,120,150 day after lamination to the testing time, in each point in time sampling, sample warp
It is stored in low temperature refrigerator (- 70 DEG C) preservations after liquid nitrogen frozen, the measurement of embryo rate then is carried out to sample, and swash to sample is endogenous
Plain GA3, IAA are extracted and are measured.After measured, the regulation of exogenous hormone can promote the development of rhizoma polygonati embryo, while external source swashs
The regulation of element can promote the raising of rhizoma polygonati Seed Endogenous Hormones, to promote the development of rhizoma polygonati embryo, make the germinating time of rhizoma polygonati
In advance.
In summary, the present invention has effectively facilitated the germination of rhizoma polygonati seed, improves the germination percentage of rhizoma polygonati seed and neat
Degree.
Detailed description of the invention
Fig. 1 is embryo rate result.
Fig. 2 is the result of variations of endogenous hormones.
Specific embodiment
Explanation of technical terms
Heteroauxin (English name: indole-3-acetic acid, abbreviation: IAA), also known as: benzazole guanidine-acetic acid.Indoles second
Acid is the intracorporal metabolite of plant and plays regulating and controlling effect to the growth and development of plant and Dormancy And Germination.
Gibberellin (English name: Gibberellin A3, abbreviation: GA3), gibberellin be also the intracorporal metabolite of plant simultaneously
Growth and development and Dormancy And Germination to plant play regulating and controlling effect.
By the research to Dormancy Mechanism, it is found that plant hormone adjusting is a main aspect.Modern studies have found that
Gibberellin and the basic element of cell division are that germination promotes substance.In general, abscisic acid is dominant in the seed of suspend mode, when suspend mode by
Then dominances such as gibberellin when gradually releasing.During 5 DEG C of wet sand laminations, abscisic acid, gibberellin and 3 kinds of the basic element of cell division swash seed
The changes of contents situation of element: abscisic acid tends to reduce, and gibberellin and the basic element of cell division are by without to gradually increasing.
Gibberellin can promote seed to sprout, and it is by release and to increase alpha-amylase that experiment, which shows that gibberellin promotes to sprout,
Activity realize.There is the Cereal seed of germination, after seed soaking, embryo portion discharges gibberellin, it enters aleurone, promotes each
The synthesis of kind of enzyme, be hydrolyzed into reserve substance for embryonic development small molecule compound.Most compellent experiment is wheat
Kind cuts in half, and has one end of embryo is under water to can produce alpha-amylase, hydrolyzes starch saccharogenesis.One end of no embryo is under water
Then without this variation, the end of no embryo, which is immersed in gibberellin aqueous solution, can also generate this enzyme, hydrolyze starch.It is such as water-soluble in gibberellin
The amino acid of label 14C is added in liquid, then finds that 14C is mixed by polypeptide chain in the amino acid of composition amylase, illustrates red mould
Element can promote the synthesis of protein.Experiment at present, which shows at least to have in seed endosperm 10 kinds or more of enzyme, to be gibberellin institute
Induce and release, including degradation glucoside, lactose, cellobiose, fish rib albumen, peptide, glucose, maltose and
The various enzymes such as sucrose.Thus, gibberellin be referred to as capable of mobilizing and using Endosperm hormone.
The basic element of cell division can promote cell division and adjust cell differentiation.The study found that the basic element of cell division is to releasing seed
For suspend mode, the mode of action be by the interaction of the basic element of cell division/abscisic acid as a result, with regulate and control the permeability level of film and
The release of gibberellin is promoted to realize.The basic element of cell division can transfer the effect of gibberellin while " solution inhibits ", it is known that red
Mycin is the privileged site for being fixed on embryo portion cell in static seed and resting seed, and " gibberellin " to be employed of seed is necessary
It releases from " isolation ward ", is flow on position with free state, can just play due physiological function.
Experimental example 1
1, experiment purpose: seed embryo rate and the dynamic of endogenous hormones in observation rhizoma polygonati kind embryo dormancy of the present invention and growth course
Variation.
2, materials and methods
2.1 material
2.1.1 seed: the mature seed of the rhizoma polygonati of in October, 20014 harvesting.
2.1.2 kit: using enzyme-linked immune analytic method (ELISA), and kit is purchased from China Agricultural University, by reagent
Box illustrates the content for measuring GA3, IAA in sample.
2.1.3 instrument: the full-automatic multiplexing function microplate reader of the vigorous Multiskan MK3 of Finland's thunder；U.S.'s Beckman
AllegraTM 25R centrifuge refrigerated centrifuge；Switzerland's PT1600E refiner；Switzerland's plum Teller AX205 balance.
2.2 method
2.2.1 processing method:
Test kind is the fresh seeds harvested.
The fresh seeds are placed 5 days in shady place, clear water impregnates 2 hours, removes kind of a skin, cleans, dry in the shade seed coat
Water after with the clean fine sand lamination of 1:3 in seed culture ware, lamination sand water content be 70%.Lamination temperature and time: 0~
30 days, 10 ± 1 DEG C；Seed is taken out, sand is gone to clean, with 2 hours pavilions of GA3 seed soaking of 100mg/L, places 20 ± 1 DEG C of plant growths
30~90 days in case；Seed is taken out, sand is gone to clean, is soaked seed 2 hours with the IAA of concentration 25mg/L, it is raw to place 20 ± 1 DEG C of plants
90~150 days in long case.Testing time is 0,30,60,90,120,150 day after lamination.In each point in time sampling, sample is through liquid
(- 70 DEG C) preservations are stored in low temperature refrigerator after chilled nitrogen, in case test.
2.2.2 the measurement of embryo rate: in different Stratification time points, taking 30 seeds every time, cuts endosperm along embryo center
Into two.With the length (mm) of micrometer measurement embryo and endosperm under binocular anatomical lens.
Calculate embryo rate: embryo rate=embryo length/endosperm is long, seeks the average embryo rate of each time point.
2.2.3 the extraction and measurement of endogenous hormones GA3, IAA
The extraction of hormone: the sample 1.0g that precision weighs each time point adds 4ml sample extracting solution, in ice bath in mortar
In be ground into homogenate, extract 4h at 4 DEG C, 1000g is centrifuged 15min.Supernatant crosses C18 rubber column gel column, step are as follows: 80% equilibrium methanol glue
Column-loading-collection sample -, which is removed after sample to wash -100% ether of column with 100% methanol and wash -100% methanol of column, washes column-circulation.
Sample is removed the methanol in extracting solution, is settled to 1ml with sample diluting liquid with being dried with nitrogen after column will be crossed.
The measurement of hormone: using enzyme-linked immune analytic method (ELISA), and kit is purchased from China Agricultural University, by reagent
Box illustrates the content for measuring GA3, IAA in sample.
2.2.4 statistical disposition: all data are indicated with average.
3 results
3.1 embryo rate results: such as Fig. 1
The result of variations of 3.2 endogenous hormones: such as Fig. 2
The analysis of 4 data
The results show that the regulation of exogenous hormone can promote the development of rhizoma polygonati embryo, while the regulation of exogenous hormone can promote
The raising of rhizoma polygonati Seed Endogenous Hormones shifts to an earlier date the germinating time of rhizoma polygonati to promote the development of rhizoma polygonati embryo.
Claims (2)
1. a kind of germination accelerating method of rhizoma polygonati seed, which is characterized in that the germination accelerating method includes:
1) Stratificated treatment is carried out to seed, Stratificated treatment condition includes: that lamination sand water content is 70%, and lamination temperature is 10 ± 1
DEG C, lamination duration are as follows: 0-30 days；
2) seed is taken out, sand is gone to clean, is soaked seed 2 hours with the GA3 of 100mg/L, is then placed in 20 ± 1 DEG C of growth chambers
30-90 days；
3) seed is taken out, sand is gone to clean, is soaked seed 2 hours with the IAA of concentration 25mg/L, then places 20 ± 1 DEG C of growth chambers
Middle 90-150 days.
2. the germination accelerating method of rhizoma polygonati seed according to claim 1, it is characterised in that: carried out at lamination to the seed
It further include carrying out shady place to seed to place 5 days, clear water impregnates 2 hours, removes kind of a skin, cleans, dry in the shade seed coat before reason
The step of water.
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Citations (5)
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| KR20120095753A (en) * | 2011-02-21 | 2012-08-29 | 순천대학교 산학협력단 | Culture media for mushroom cultivation comprising herbal slurgies and mushroom having the activity of anticancer and immunological enhancement produced thereby |
| CN104737665A (en) * | 2015-03-05 | 2015-07-01 | 云南省农业科学院药用植物研究所 | Method for germinating polygonatum kingianum seeds |
| CN104920198A (en) * | 2015-07-14 | 2015-09-23 | 安徽省应用技术研究院 | Seed germination acceleration seedling culturing method for polygonatum cyrtonema |
| CN107864861A (en) * | 2017-12-12 | 2018-04-03 | 安徽农业大学 | A kind of tissue culture and rapid propagation method of David's-harp |
| CN109089463A (en) * | 2018-08-09 | 2018-12-28 | 重庆市银蜂四云创圆农业开发有限公司 | A kind of polygonatum cyrtonema seed growing method that the service life can be improved |
-
2019
- 2019-05-20 CN CN201910419345.3A patent/CN110402637A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120095753A (en) * | 2011-02-21 | 2012-08-29 | 순천대학교 산학협력단 | Culture media for mushroom cultivation comprising herbal slurgies and mushroom having the activity of anticancer and immunological enhancement produced thereby |
| CN104737665A (en) * | 2015-03-05 | 2015-07-01 | 云南省农业科学院药用植物研究所 | Method for germinating polygonatum kingianum seeds |
| CN104920198A (en) * | 2015-07-14 | 2015-09-23 | 安徽省应用技术研究院 | Seed germination acceleration seedling culturing method for polygonatum cyrtonema |
| CN107864861A (en) * | 2017-12-12 | 2018-04-03 | 安徽农业大学 | A kind of tissue culture and rapid propagation method of David's-harp |
| CN109089463A (en) * | 2018-08-09 | 2018-12-28 | 重庆市银蜂四云创圆农业开发有限公司 | A kind of polygonatum cyrtonema seed growing method that the service life can be improved |
Non-Patent Citations (3)
| Title |
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| 《今日黑土地》栏目组: "《农家生活百事通》", 31 January 2014, 辽宁科学技术出版社 * |
| 卢宝伟: "《药用植物种苗繁育概论》", 31 May 2018, 中国海洋大学出版社 * |
| 杨龙章等: "《山楂栽培技术问答》", 30 June 1988, 辽宁大学出版社 * |
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