CN103766040A - Method for improving seed germination rate of wild aconitum gymnandrum maxim - Google Patents
Method for improving seed germination rate of wild aconitum gymnandrum maxim Download PDFInfo
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- CN103766040A CN103766040A CN201410020367.XA CN201410020367A CN103766040A CN 103766040 A CN103766040 A CN 103766040A CN 201410020367 A CN201410020367 A CN 201410020367A CN 103766040 A CN103766040 A CN 103766040A
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- nakedstamen
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Abstract
The invention discloses a method for improving seed germination rate of wild aconitum gymnandrum maxim. The method comprises the steps of removing shells of seeds of the wild aconitum gymnandrum maxim, disinfecting, and soaking the seeds in 1000mg/ L gibberellin solution for 24h; respectively carrying out variable temperature cultivation at 18-25 DEG C for 12h in the light environment and dark environment to promote seed germination. The method has the beneficial effects that plant hormone GA3 is used for treating the seeds of the wild aconitum gymnandrum maxim, so that in the light environment, the seed germination rate of the aconitum gymnandrum maxim is increased to 91.7% from 7%, and in the dark environment, the seed germination rate of the aconitum gymnandrum maxim is increased to 95.7%. The method has the advantages that the method is simple and economical, is not influenced by factors such as seasons, climate and natural disasters; after the technology is utilized, the problems that the seeds of the aconitum gymnandrum maxim are low in reproductive capacity and short of resource can be solved, the demand for the seeds of the aconitum gymnandrum maxim can be met by artificial domestication cultivation, and the method has important significance for protecting the natural resource and natural ecological environment of the wild aconitum gymnandrum maxim.
Description
Technical field
The present invention relates to improve the method for seed germination rate, be specifically related to a kind of method that improves wild Root of Nakedstamen Monkshood seed germination rate.
Background technology
Root of Nakedstamen Monkshood
(Aconitum gymnandrum Maxim.)it is Ranunculaceae (Ranunculaceae) delphinium grandiflorum family (tribe Delphinieae) Aconitum
(Aconitum L.)unique species of Root of Nakedstamen Monkshood subgenus (subgen. Gymnaconitum (Stapf) Rapaics), only be distributed in China-Himalaya plant subprovince system and neighbour area, mainly be distributed in Tibet, western Sichuan, Qinghai, SOUTH OF GANSU, grow in mountain region Cao Po, meadow, limit, field and river bank sandy ground between height above sea level 1550-3800 m.The rare traditional Chinese medicine affiliations such as Root of Nakedstamen Monkshood and monkshood, radix aconiti agrestis approach, and its leaf, flower, root all can be used as medicine, and taste is pungent, warm in nature, and the tool effect that maggot is killed in calmness, expelling parasite of dispeling the wind, can be used for treating the disease such as arthralgia, rheumatism, and herb is poisonous.
But in recent years; because the medicinal and economic worth of Root of Nakedstamen Monkshood is excessively excavated or the exploitation of predation formula; it has faced resource atrophy gradually; grassland degeneration; the situation that grassland ecology balance is destroyed; therefore, the protection of reinforcement Wild Medicinal Plants Resources has become current important subject with reasonable and artificial domesticating cultivation.We study discovery, the very low primary factor that limits its procreation and cultivation that become of wild Root of Nakedstamen Monkshood seed germination rate, at present in wild Root of Nakedstamen Monkshood research, the overwhelming majority concentrates on the aspect such as active ingredient and pharmacological effect, and little with the research report of abolishing method aspect about the broken dormancy mechanism of wild Root of Nakedstamen Monkshood seed.Gibberellin GA
3as a Plant Hormone, there is the effect of the dormancy of abolishing, in the research of other plant and seed germination, there is relevant research report, and in the broken dormancy mechanism of wild Root of Nakedstamen Monkshood with abolish aspect method and also rarely have research.
Summary of the invention
The object of the invention is for above-mentioned defect of the prior art, provide a kind of by gibberellin processing, thereby improve the method for wild Root of Nakedstamen Monkshood seed germination rate.
To achieve these goals, technical scheme provided by the invention is: a kind of method that improves wild Root of Nakedstamen Monkshood seed germination rate is that wild Root of Nakedstamen Monkshood seed is shelled after sterilization.Be gibberellin (GA3) the solution immersion Root of Nakedstamen Monkshood seed 24h of 1000mg/ L by concentration, respectively under illumination and dark condition, 18 ℃ of (12h)～25 (12h) ℃ Fluctuation temperature culture is extremely sprouted, thereby improves the method for wild Root of Nakedstamen Monkshood seed germination rate.
Further, a kind of above-mentioned method that improves wild Root of Nakedstamen Monkshood seed germination rate, comprises the following steps:
1) get the seed of wild Root of Nakedstamen Monkshood, seed is air-dry, manually remove shell, under low temperature, dry environment, preserve, Root of Nakedstamen Monkshood seed obtains shelling;
2) step 1) is obtained shelling Root of Nakedstamen Monkshood seed is selected, and selects Root of Nakedstamen Monkshood seeds in the same size, full grains, first uses liquor natrii hypochloritis's sterilization 5-10min of 5%, then with distilled water flushing 2-3 time, Root of Nakedstamen Monkshood seed after being sterilized;
3) by step 2) Root of Nakedstamen Monkshood seed soaks 24h in 1000mg/L Gibberellins solution after the sterilization that obtains, is then placed in the culture dish that is lined with two layers of filter paper, and every ware is placed 100, is provided with three repetitions, Root of Nakedstamen Monkshood seed after obtaining gibberellin and processing;
4) gibberellin step 3) being obtained is processed rear Root of Nakedstamen Monkshood seed and is placed in two incubators, respectively in 3000LX illumination and dark, under ℃ temperature match curing conditions of 18 ℃ of (12h)～25 (12h), cultivates, and can obtain sprouting Root of Nakedstamen Monkshood germination seed.
Second object of the present invention has been to provide gibberellin in the application improving in wild Root of Nakedstamen Monkshood seed germination rate.
Beneficial effect of the present invention is: the present invention utilizes plant hormone GA
3process the method breaking dormancy of wild Root of Nakedstamen Monkshood seed; its advantage is: under illumination; can make Root of Nakedstamen Monkshood seed germination rate bring up to 91.7% from original 7%; under dark condition, bring up to 95.7%; method is simple; economical; be not subject to the impact of the factors such as season, weather, natural calamity; utilize that this technology can solve that Root of Nakedstamen Monkshood seminal propagation ability is low, the kind Geological Problems of scarcity of resources, artificial domesticating cultivation, to preserving the ecological environment, protect the aspect such as germplasm and artificial cultivation significant.
Embodiment
embodiment 1:
A method that improves wild Root of Nakedstamen Monkshood seed germination rate, comprises the following steps:
1, test material is wild Root of Nakedstamen Monkshood seed, adopts the Zhua Xi grassland, Tianzhu, Gansu Province in height above sea level 2960-2970m, on the plant of Root of Nakedstamen Monkshood, directly gather, after seed is air-dry, manually remove shell, under low temperature, dry environment, save backup;
2, select in the same size, full Root of Nakedstamen Monkshood seed, first use liquor natrii hypochloritis's sterilization 5min of 5%, then use distilled water flushing 2 times, by Root of Nakedstamen Monkshood seed at 1000 mg/L GA
3in solution, soak 24h, be then placed in the culture dish (φ 9cm) that is lined with two layers of filter paper, every ware is placed 100, and each concentration is established three repetitions;
3, all culture dishes are placed in to two incubators, respectively in 3000LX illumination and dark, under ℃ temperature match curing conditions of 18 ℃ of (12h)～25 (12h), cultivate, show money or valuables one carries unintentionally as sprouting mark using seed, add up germination rate every day, add up altogether 8d, 5d measures germination vigor, and 8d measures germination rate, germination index and vitality index;
4, it is as follows that seed germination is measured formula:
Germination rate (%)=(subnumber is planted experimentally in chitting piece number/confession) × 100%;
Germination vigor (%)=(seed grain number/confession chitting piece grain number × 100% germinateing in 5d);
Germination index (Gi)=∑ Gt/Dt; The germinative number that wherein Gt is 8d, Dt is for germinateing day;
Vitality index (VI)=germination index × seedling mean fresh (g).
5, GA
3process extremely significantly promoted Root of Nakedstamen Monkshood seed sprouting (
p﹤ 0.01), table 1 and 2 shows, the germination rate impinging upon under illumination and dark condition is only respectively to 7% and 24.3%, at variable concentrations GA
3in processing, the most remarkable with the raising of 1000mg/L germination rate, GA
3when concentration 1000mg/L+ illumination, germination rate can be brought up to germination rate under 91.7%, 1000mg/L+ dark condition and reaches 95.7%, through the GA of 1000mg/L
3its germination vigor of Root of Nakedstamen Monkshood seed, germination index of processing all the utmost point be significantly higher than contrast (
p﹤ 0.01), under illumination condition, exceed than contrast respectively: 48.7%, 16.3%; Under dark condition, exceed respectively: 74.3%, 17.6%.Wherein, table 1 is variable concentrations GA
3the impact of solution on Root of Nakedstamen Monkshood seed germination rate under illumination condition, table 2 is the impact of variable concentrations GA3 solution on Root of Nakedstamen Monkshood seed germination rate under dark condition.
Table 1
Table 2
。
Note: the different capitalizations of same column represent difference extremely significantly (
p﹤ 0.01), different lowercase alphabets show significant difference (
p﹤ 0.05).
embodiment 2:
Embodiment is identical with embodiment 1, and institute's difference is, in step 2, with liquor natrii hypochloritis's sterilization 10min of 5%, then uses distilled water flushing 3 times.
embodiment 3:
Embodiment is identical with embodiment 1, and institute's difference is, in step 2, with liquor natrii hypochloritis's sterilization 8min of 5%, then uses distilled water flushing 3 times.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (3)
1. a method that improves wild Root of Nakedstamen Monkshood seed germination rate, is characterized in that, is that wild Root of Nakedstamen Monkshood seed is shelled after sterilization, with Gibberellins solution processing, sprouts processing respectively in illumination and dark condition.
2. a kind of method that improves wild Root of Nakedstamen Monkshood seed germination rate according to claim 1, is characterized in that, comprises the following steps:
1) get the seed of wild Root of Nakedstamen Monkshood, seed is air-dry, manually remove shell, under low temperature, dry environment, preserve, Root of Nakedstamen Monkshood seed obtains shelling;
2) step 1) is obtained shelling Root of Nakedstamen Monkshood seed is selected, and selects Root of Nakedstamen Monkshood seeds in the same size, full grains, first uses liquor natrii hypochloritis's sterilization 5-10min of 5%, then with distilled water flushing 2-3 time, Root of Nakedstamen Monkshood seed after being sterilized;
3) by step 2) Root of Nakedstamen Monkshood seed soaks 24h in 1000mg/L Gibberellins solution after the sterilization that obtains, is then placed in the culture dish that is lined with two layers of filter paper, and every ware is placed 100, is provided with three repetitions, Root of Nakedstamen Monkshood seed after obtaining gibberellin and processing;
4) the rear Root of Nakedstamen Monkshood seed of gibberellin processing step 3) being obtained is placed in two illumination boxs and cultivates, respectively in 3000LX illumination and dark, under ℃ temperature match curing conditions of 18 ℃ of (12h)～25 (12h), cultivate, can obtain sprouting Root of Nakedstamen Monkshood germination seed.
3. gibberellin is in the application improving in wild Root of Nakedstamen Monkshood seed germination rate.
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| CN201410020367.XA CN103766040B (en) | 2014-01-17 | 2014-01-17 | A kind of method improving wild Root of Nakedstamen Monkshood seed germination rate |
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| CN103766040B CN103766040B (en) | 2016-04-06 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365212A (en) * | 2014-09-12 | 2015-02-25 | 甘肃省治沙研究所 | Method for breaking psammophyte Agriophyllum squarrosum seed dormancy and improving germination rate |
| CN104541665A (en) * | 2015-01-23 | 2015-04-29 | 中国农业科学院特产研究所 | Method for breaking dormancy of aconitum kusnezoffii reichb seeds |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09220002A (en) * | 1996-02-16 | 1997-08-26 | T S Shokubutsu Kenkyusho:Kk | Method for improving germination of seed |
| CN101099422A (en) * | 2007-07-25 | 2008-01-09 | 毕慧滨 | Method for improving iris lactea seed germination rate |
-
2014
- 2014-01-17 CN CN201410020367.XA patent/CN103766040B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09220002A (en) * | 1996-02-16 | 1997-08-26 | T S Shokubutsu Kenkyusho:Kk | Method for improving germination of seed |
| CN101099422A (en) * | 2007-07-25 | 2008-01-09 | 毕慧滨 | Method for improving iris lactea seed germination rate |
Non-Patent Citations (1)
| Title |
|---|
| 周丽霞: ""乌头属部分植物的资源调查及引种栽培研究"", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365212A (en) * | 2014-09-12 | 2015-02-25 | 甘肃省治沙研究所 | Method for breaking psammophyte Agriophyllum squarrosum seed dormancy and improving germination rate |
| CN104541665A (en) * | 2015-01-23 | 2015-04-29 | 中国农业科学院特产研究所 | Method for breaking dormancy of aconitum kusnezoffii reichb seeds |
| CN104541665B (en) * | 2015-01-23 | 2017-04-12 | 中国农业科学院特产研究所 | Method for breaking dormancy of aconitum kusnezoffii reichb seeds |
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