CN106171983A - A kind of cultural method of sword-leaved cymbidium test tube flowering - Google Patents
A kind of cultural method of sword-leaved cymbidium test tube flowering Download PDFInfo
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- CN106171983A CN106171983A CN201610545441.9A CN201610545441A CN106171983A CN 106171983 A CN106171983 A CN 106171983A CN 201610545441 A CN201610545441 A CN 201610545441A CN 106171983 A CN106171983 A CN 106171983A
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- sword
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract
The present invention relates to technical field of plant culture; it is specifically related to the cultural method of a kind of sword-leaved cymbidium test tube flowering; the method comprises the following steps: material primary election, preculture, Flower induction pretreatment and induced flowering; the sword-leaved cymbidium Seedling strain flower induction rate cultivated by this method is high, grow fine; pass through animal nutrition simultaneously; meet the demand commercially produced, cultivate the sword-leaved cymbidium that blooms provide technical support for enterprise scale, industrialization, meet the needs in sword-leaved cymbidium market of blooming simultaneously.
Description
Technical field
The present invention relates to technical field of plant culture, be specifically related to the cultural method of a kind of sword-leaved cymbidium test tube flowering.
Background technology
Sword-leaved cymbidium be raw plant, be born under sparse woods, in shrubbery, mountain valley is other or in thick grass, height above sea level 600-1800 rice.Produce China
Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Hunan, Guangdong, Hainan, Guangxi, South-west Sichuan, Guizhou and Yunnan.It is distributed widely in
Southeast Asia and South Asia various countries, north is to Japan.Cymbidium ensifolium (L.) Sw. breeding potential under field conditions (factors) is extremely low and blooms more difficult, therefore test tube flowering
Applying to the most more and more in Cymbidium ensifolium (L.) Sw. cultivation, described test tube flowering is bloomed the most in vitro, is the means of utilization group training, makes plant
This physiological status of blooming can be realized during isolated culture.The test tube flowering research of orchid, not only to Cymbidium ensifolium (L.) Sw.
Bloom process, bloom mechanism research significant, the also breeding work to Cymbidium ensifolium (L.) Sw., in advance understand educated new varieties character
And the technical support useful to the commercial value offer of orchid production.But have not been reported in terms of sword-leaved cymbidium test tube flowering and
Use.
Summary of the invention
It is an object of the invention to provide the cultivation side of the sword-leaved cymbidium test tube flowering that a kind of flower induction rate is high, grow fine
Method.
In order to achieve the above object, the technical solution used in the present invention is: the cultural method of a kind of sword-leaved cymbidium test tube flowering, bag
Include following steps:
(1) material primary election: choose the sword-leaved cymbidium test tube Seedling grown fine without pest and disease damage, test tube height of seedling is 3～6cm, root 3～6
Bar, leaf 2～3；
(2) preculture: the sword-leaved cymbidium test tube Seedling after choosing is placed in initial culture base to be cultivated, and incubation time is 50
～60d, cultivation temperature is 22～25 DEG C, and light application time is 14h/d, and intensity of illumination is 1600～2000lx, within every 10 days, changes one
Secondary initial culture base, described initial culture base is: MS+1～3mg/L6-BA+0.1～0.2mg/L NAA+28～32g/L sucrose+
5～9g/L agar+0.1～0.2g/L activated carbons；
(3) Flower induction pretreatment: grow after choosing preculture unanimously, growing way is healthy, sword-leaved cymbidium Seedling strain that is that pollute without insect pest
Being placed in pre-culture and carry out preculture, incubation time is 22～27d, and cultivation temperature is 22～25 DEG C, and light application time is
14h/d, intensity of illumination is 1600～2000lx, and described pre-culture is: MS+2～4mg/L PP333+ 28～32g/L sucrose+5
～9g/L agar+0.1～0.2g/L activated carbon；
(4) induced flowering: Seedling strain root pretreated to Flower induction is pruned, is then placed within induced flowering training
Supporting in base and cultivate, incubation time is 100～120d, and cultivation temperature is 22～25 DEG C, and light application time is 14h/d, and illumination is strong
Degree is 1600～2000lx, every 30 days change an induced flowering culture medium, described induced flowering culture medium is: MS+0.3～
0.5mg/L TDZ+0.1～0.2mg/L 2,4-D+0.8～1.2mg/L ZT+28～32g/L sucrose+5～9g/L agar+0.1
～0.2g/L activated carbon.
The cultural method of a kind of sword-leaved cymbidium test tube flowering as above, further illustrate into, described initial culture base is: MS
+ 1.5mg/L 6-BA+0.15mg/L NAA+30g/L sucrose+6g/L agar+0.15g/L activated carbon.
The cultural method of a kind of sword-leaved cymbidium test tube flowering as above, further illustrate into, described pre-culture is: MS+
3mg/L PP333+ 30g/L sucrose+6g/L agar+0.15g/L activated carbon.
The cultural method of a kind of sword-leaved cymbidium test tube flowering as above, further illustrates as, described induced flowering culture medium
For: MS+0.4mg/L TDZ+0.1mg/L 2,4-D+1.0mg/L ZT+30g/L sucrose+6g/L agar+0.15g/L activated carbon.
The invention has the beneficial effects as follows: this method can be greatly improved the flower induction rate of sword-leaved cymbidium, wherein by adding
PP333The Flower induction rate of Seedling strain can be increased so that the quantity showed increased of flower, by TDZ, 2, the group between 4-D and ZT
Closing, it is possible to promote the formation of bud, flower_bud formation is very fast and bud is open normally, the florescence is long, grows fine.Simultaneously by biology
Technological means, meets the demand commercially produced, and cultivates the sword-leaved cymbidium that blooms and provides technology for enterprise scale, industrialization and prop up
Hold, meet the needs in sword-leaved cymbidium market of blooming simultaneously.
Detailed description of the invention
Below the specific embodiment of the invention is further elaborated.
The invention provides the cultural method of a kind of sword-leaved cymbidium test tube flowering, it is adaptable to sword-leaved cymbidium uses.The method specifically includes
Following steps:
(1) material primary election: choose the sword-leaved cymbidium test tube Seedling grown fine without pest and disease damage, test tube height of seedling is 3～6cm, root 3～6
Bar, leaf 2～3；Test tube Seedling does not limits, and can be that tissue culture comes, it is also possible to cultivate for plant division；Choose without disease pest
The sword-leaved cymbidium that evil grows fine, primarily to improve the flower induction rate of sword-leaved cymbidium, thus reduces cost, improves efficiency.
(2) preculture: the sword-leaved cymbidium test tube Seedling after choosing is placed in initial culture base to be cultivated, and incubation time is 50
～60d, cultivation temperature is 22～25 DEG C, and light application time is 14h/d, and intensity of illumination is 1600～2000lx, within every 10 days, changes one
Secondary initial culture base, described initial culture base is: MS+1～3mg/L 6-BA+0.1～0.2mg/L NAA+28～32g/L sucrose
+ 5～9g/L agar+0.1～0.2g/L activated carbons；Make to again filter out underproof sword-leaved cymbidium Seedling strain by preculture, from
And improve efficiency and the success rate of this method, simultaneously by the preculture of a period of time, it is possible to make Seedling strain more healthy and stronger, improve Seedling
The survival rate of strain；
Table 1 is the various embodiments of initial culture base
Table 1 gives the embodiment of 6 kinds of different initial culture bases, in the amount ranges of this initial culture base, also may be used
There to be other embodiments, it is illustrated the most one by one；As preferably, described initial culture base is: MS+1.5mg/L 6-BA+
0.15mg/L NAA+30g/L sucrose+6g/L agar+0.15g/L activated carbon, is composition and the use of initial culture base 1 in table 1
Amount.
(3) Flower induction pretreatment: grow after choosing preculture unanimously, growing way is healthy, sword-leaved cymbidium Seedling strain that is that pollute without insect pest
Being placed in pre-culture and carry out preculture, incubation time is 22～27d, and cultivation temperature is 22～25 DEG C, and light application time is
14h/d, intensity of illumination is 1600～2000lx, and described pre-culture is: MS+2～4mg/L PP333+ 28～32g/L sucrose+5
～9g/L agar+0.1～0.2g/L activated carbon；Pass through PP333Seedling strain apical growth advantage can be stoped, promote that lateral bud grows, delay
Plant growing, suppression cane elongation, promote bud differentiation, thus increase the quantity of differentiation of bud, make the test tube flowering rate of sword-leaved cymbidium
Significantly improve.
Table 2 is the various embodiments of pre-culture
Table 2 gives the embodiment of 6 kinds of different pre-culture, in the amount ranges of this pre-culture, it is also possible to have
Other embodiments, are illustrated the most one by one；As preferably, described pre-culture is: MS+3mg/L PP333+ 30g/L sucrose
+ 6g/L agar+0.15g/L activated carbon, is composition and the consumption of pre-culture 1 in table 2.
Table 3 is the PP of variable concentrations333The pretreatment impact on test tube Seedling Flower induction
| Process numbering | PP333(mg/L) | Result |
| 1 | 1 | Single bud, bud is little |
| 2 | 2 | Some Flos Lonicerae bud, bud is bigger |
| 3 | 3 | Flos Lonicerae bud occupies the majority, and bud is bigger |
| 4 | 4 | Some Flos Lonicerae bud, bud is bigger |
| 5 | 5 | Plant is downgraded, bud deformity |
Table 3 use same pre-culture cultivate, simply PP333Concentration is different, so that it is guaranteed that the standard of controlled trial
Really property.From table 3 it is observed that PP333The too small induction to bud of concentration is without obvious effect, PP333Concentration is excessive, most test tube
There is dwarfism in Seedling, it is suppressed that the growth of Seedling strain, described PP333It is preferably 2～4mg/L；
(4) induced flowering: Seedling strain root pretreated to Flower induction is pruned, mainly stripling strain root
Rotten and unnecessary side root, be then placed within induced flowering culture medium cultivating, and incubation time is 100～120d, cultivates
Temperature is 22～25 DEG C, and light application time is 14h/d, and intensity of illumination is 1600～2000lx, within every 30 days, changes an induced flowering
Culture medium, described induced flowering culture medium is: MS+0.3～0.5mg/L TDZ+0.1～0.2mg/L 2,4-D+0.8～
1.2mg/L ZT+28～32g/L sucrose+5～9g/L agar+0.1～0.2g/L activated carbon；By adding TDZ, it is possible to increase flower
Bud induction rate, make sword-leaved cymbidium grow fine simultaneously, leaf dark green, aerial root less, in addition, TDZ can make Seedling strain base portion continuous
Differentiation generates protocorms, continues to cultivate and can bear intensive differentiation plantlet, increases the expanding propagation coefficient of sword-leaved cymbidium；2,4-D and
ZT can make flower_bud formation very fast, and the open compared with normal of bud, florescence are long, the more conducively test tube flower induction of sword-leaved cymbidium；
Table 4 is the various embodiments of induced flowering culture medium
Table 4 gives the embodiment of 6 kinds of different induced flowering culture medium, in the amount ranges of this pre-culture, also
Can there be other embodiments, be illustrated the most one by one；As preferably, described induced flowering culture medium is: MS+0.4mg/L
TDZ+0.1mg/L 2,4-D+1.0mg/L ZT+30g/L sucrose+6g/L agar+0.15g/L activated carbon, is in table 4 induction and opens
The composition of spent culture medium 1 and consumption.
By the animal nutrition of this sword-leaved cymbidium test tube flowering, meet the demand commercially produced, for enterprise scale,
Industrialization is cultivated the sword-leaved cymbidium that blooms and is provided technical support, meets the needs in sword-leaved cymbidium market of blooming simultaneously.
The present invention is not limited to examples detailed above, in claims of the present invention limited range, and art technology
Various deformation or amendment that personnel can make without creative work are all protected by this patent.
Claims (4)
1. the cultural method of a sword-leaved cymbidium test tube flowering, it is characterised in that comprise the following steps:
(1) material primary election: choose the sword-leaved cymbidium test tube Seedling grown fine without pest and disease damage, test tube height of seedling is 3～6cm, root 3～6, leaf
2～3；
(2) preculture: the sword-leaved cymbidium test tube Seedling after choosing is placed in initial culture base to be cultivated, incubation time be 50～
60d, cultivation temperature is 22～25 DEG C, and light application time is 14h/d, and intensity of illumination is 1600～2000lx, within every 10 days, changes once
Initial culture base, described initial culture base is: MS+1～3mg/L 6-BA+0.1～0.2mg/L NAA+28～32g/L sucrose+5
～9g/L agar+0.1～0.2g/L activated carbon；
(3) Flower induction pretreatment: choose the sword-leaved cymbidium Seedling strain placement that growth after preculture is consistent, growing way is healthy, pollute without insect pest
Carrying out preculture in pre-culture, incubation time is 22～27d, and cultivation temperature is 22～25 DEG C, and light application time is 14h/d,
Intensity of illumination is 1600～2000lx, and described pre-culture is: MS+2～4mg/LPP333+ 28～32g/L sucrose+5～9g/L fine jades
Fat+0.1～0.2g/L activated carbon；
(4) induced flowering: Seedling strain root pretreated to Flower induction is pruned, and is then placed within induced flowering culture medium
In cultivate, incubation time is 100～120d, and cultivation temperature is 22～25 DEG C, and light application time is 14h/d, and intensity of illumination is
1600～2000lx, within every 30 days, change an induced flowering culture medium, described induced flowering culture medium is: MS+0.3～0.5mg/
L TDZ+0.1～0.2mg/L 2,4-D+0.8～1.2mg/L ZT+28～32g/L sucrose+5～9g/L agar+0.1～0.2g/
L activated carbon.
The cultural method of a kind of sword-leaved cymbidium test tube flowering the most according to claim 1, it is characterised in that: described initial culture base
For: MS+1.5mg/L 6-BA+0.15mg/L NAA+30g/L sucrose+6g/L agar+0.15g/L activated carbon.
The cultural method of a kind of sword-leaved cymbidium test tube flowering the most according to claim 1, it is characterised in that: described pre-culture
For: MS+3mg/L PP333+ 30g/L sucrose+6g/L agar+0.15g/L activated carbon.
The cultural method of a kind of sword-leaved cymbidium test tube flowering the most according to claim 1, it is characterised in that: described induced flowering is trained
Foster base is: MS+0.4mg/L TDZ+0.1mg/L 2,4-D+1.0mg/L ZT+30g/L sucrose+6g/L agar+0.15g/L activity
Charcoal.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108849504A (en) * | 2018-06-20 | 2018-11-23 | 福建农林大学 | A method of hybridization sword-leaved cymbidium rhizomes floral bud induction is at colored |
| CN109644872A (en) * | 2019-01-29 | 2019-04-19 | 福建省林业科技试验中心 | It is a kind of to hybridize the cultural method bloomed in sword-leaved cymbidium bottle |
| CN111066655A (en) * | 2019-12-24 | 2020-04-28 | 浙江海丰花卉有限公司 | Method for culturing flowering in multi-head cut chrysanthemum bottle |
| CN111642393A (en) * | 2020-04-30 | 2020-09-11 | 广西壮族自治区农业科学院花卉研究所 | Tissue culture method for inducing flowering in cymbidium sinense bottle |
-
2016
- 2016-07-12 CN CN201610545441.9A patent/CN106171983A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108849504A (en) * | 2018-06-20 | 2018-11-23 | 福建农林大学 | A method of hybridization sword-leaved cymbidium rhizomes floral bud induction is at colored |
| CN109644872A (en) * | 2019-01-29 | 2019-04-19 | 福建省林业科技试验中心 | It is a kind of to hybridize the cultural method bloomed in sword-leaved cymbidium bottle |
| CN111066655A (en) * | 2019-12-24 | 2020-04-28 | 浙江海丰花卉有限公司 | Method for culturing flowering in multi-head cut chrysanthemum bottle |
| CN111642393A (en) * | 2020-04-30 | 2020-09-11 | 广西壮族自治区农业科学院花卉研究所 | Tissue culture method for inducing flowering in cymbidium sinense bottle |
| CN111642393B (en) * | 2020-04-30 | 2023-08-29 | 广西壮族自治区农业科学院 | Tissue culture method for inducing flowering in cymbidium bottle |
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Application publication date: 20161207 |