CN107980634A - A kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation - Google Patents
A kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation Download PDFInfo
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- CN107980634A CN107980634A CN201711350938.6A CN201711350938A CN107980634A CN 107980634 A CN107980634 A CN 107980634A CN 201711350938 A CN201711350938 A CN 201711350938A CN 107980634 A CN107980634 A CN 107980634A
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- lucid asparagus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- Biotechnology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation, using lucid asparagus mature seed as material, immersion treatment is carried out with the concentrated sulfuric acid and warm water respectively, it is placed in being sprouted under suitable Thermo-Photo sensitive on the minimal medium of addition plant growth regulating substance, until the lucid asparagus sprout of stalwartness is grown, in this, as the suitable explant material of lucid asparagus tissue-culturing rapid propagation.The method of the present invention can effectively provide the suitable explant material needed for lucid asparagus tissue-culturing rapid propagation, so as to provide safeguard to be successfully established lucid asparagus tissue-cultured seedling large-scale production technology.
Description
Technical field
The present invention relates to a kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation, particularly lucid asparagus seed to sprout
The releasing of inhibiting factor and lucid asparagus seed tissue culture technique.
Technical background
Lucid asparagus [Asparagus cochinchinensis (Lour.) Merr] is Liliaceae (Liliaceae) lucid asparagus
Belong to (AsparagusL.) climb up by holding on to shape herbaceos perennial, be one of parts of generic medicinal plants kind, its root tuber is used as medicine, have enriching yin,
Moisturize, clearing lung-heat, fall fire and other effects, medicinal history is long, and medicinal material market demand is big.
Though lucid asparagus is distributed widely in Central China, northwest, the Yangtze river basin and southern various regions, because of its natural seed production
Low, kind skin hard and compact, sprout difficulty, therefore its Natural Population is minimum, more in fragmentary distribution.In addition long-term artificial predation formula is adopted
Dig, cause the species resource increasingly rare, far from medicinal material market demand is met, be badly in need of carrying out artificial growth.Due to high-quality kind
Sub- seedling breeding is the key link of Chinese medicine scaled artificial planting, and lucid asparagus natural propagation rate is very low, for many years much
Researcher is attempted by tissue culture technique batch breeding lucid asparagus seedling, but is adapted to the outer of its tissue cultures because failing to find eventually
Implant material and be difficult to success, main cause be root, stem, leaf of lucid asparagus etc. be difficult to dedifferentiation formed callus.
The content of the invention：
It is an object of the present invention to provide a kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation, present invention side
Method can effectively provide the suitable explant material needed for lucid asparagus tissue-culturing rapid propagation, so as to be successfully established lucid asparagus tissue-cultured seedling
Large-scale production technology provides safeguard.
In order to solve the above technical problems, the present invention is realized using following technical scheme：One kind obtains lucid asparagus tissue-culturing rapid propagation
The method of suitable explant, using lucid asparagus mature seed as material, carries out immersion treatment with the concentrated sulfuric acid and warm water respectively, is adding
It is placed in being sprouted under suitable Thermo-Photo sensitive on the minimal medium of plant growth regulating substance, until growing the Tianmen of stalwartness
Hibernaculum seedling, in this, as the suitable explant material of lucid asparagus tissue-culturing rapid propagation.
In the method for the foregoing acquisition suitable explant of lucid asparagus tissue-culturing rapid propagation, following steps are specifically included：
1. the lucid asparagus seed of mature and plump is placed in the concentrated sulfuric acid after pre-processing 2~20min, A product are obtained；
2. A product are transferred to 2~8h of seed soaking in 25~55 DEG C of warm water, B product are obtained；
3. the intact seed of step B product mesosperms is selected successively with 75% alcohol sterilizing, 0.5~2min, 0.1% mercuric chloride sterilizing 1
~5min, sterile water clean seed, obtain C product, spare；
4. C product, which are inoculated on MS culture mediums, carries out sprouting culture, until the lucid asparagus sprout of stalwartness is grown, in this, as day
The suitable explant material of door winter tissue-culturing rapid propagation.
In the method for the foregoing acquisition suitable explant of lucid asparagus tissue-culturing rapid propagation, the MS culture mediums content is：6-BA
1.5~2.5mgL-1、GA30~1mgL-1, sucrose 25-35gL-1With 5.8 agar 4-10gL of pH-1。
In the method for the foregoing acquisition suitable explant of lucid asparagus tissue-culturing rapid propagation, the MS culture mediums content is：6-BA
2.0mg·L-1、GA30.5mg·L-1, sucrose 30gL-1With 5.8 agar 7gL of pH-1。
In the method for the foregoing acquisition suitable explant of lucid asparagus tissue-culturing rapid propagation, the C product are inoculated on MS culture mediums
Sprouting culture is carried out, until growing the lucid asparagus sprout of stalwartness；It is lucid asparagus seed elder generation 1~5d of dark culturing, treats that seed is grown
Move under 1000~4000lx intensities of illumination and cultivate after white bud point, light application time 12hd-1, cultivation temperature is (25 ± 2)
℃。
Applicant has carried out present invention process long-term numerous studies, and part research is as follows：
Experimental example 1：The concentrated sulfuric acid pre-processes the screening of effective time
Vegetable material lucid asparagus [Asparagus cochinchinensis (Lour.) Merr] in the present embodiment is picked up from
Wuchuan Guizhou.
By the lucid asparagus seed of mature and plump be put into 98% concentrated sulfuric acid pre-process 2min, 4min, 6min, 8min,
10min, 15min, 20min, clear water, which rinses to be placed at (25 ± 2) DEG C, sprouts culture, using distilled water immersion to compare (CK),
The result (table 1) that seed is sprouted is counted after 15d.As seen from Table 1, lucid asparagus 4~8min of seed is pre-processed with the concentrated sulfuric acid, it is sprouted
Hair has preferable action effect.
The influence that 1 concentrated sulfuric acid pretreatment time of table sprouts lucid asparagus seed
Experimental example 2：The suitable water temperature of water seed soaking and time screening
By the above-mentioned lucid asparagus seed by concentrated sulfuric acid pretreatment be immediately placed in 25 DEG C, 35 DEG C, 45 DEG C, in 55 DEG C of warm water
Soak seed 2h, 4h, 6h, 8h respectively, and after treatment, which is placed at (25 ± 2) DEG C, sprouts culture, to be compareed without water seed soaking
(CK), the result (table 2) that seed is sprouted is counted after 15d.As known from Table 2, with 35 DEG C of 4~6h of water seed soaking, to lucid asparagus seed
Sprouting has preferable action effect, and than individually more preferable with the effect of dense sulfuric acid treatment.
The influence that the different water temperatures of table 2 and seed soaking time sprout lucid asparagus seed
Experimental example 3：Seed sterilization proper method is screened
By it is above-mentioned pre-processed by the concentrated sulfuric acid and lucid asparagus seed through water seed soaking with 75% alcohol sterilize 0.5min,
MS minimal mediums are inoculated in after cleaning seed with 0.1% mercuric chloride sterilizing 1min, 3min, 5min, sterile water again after 1min, 2min
On put culture sprouted at (25 ± 2) DEG C, with unsterilised to compare (CK), statistics pollution and germination result (table 3) after 15d.From
Table 3 as can be seen that with 75% alcohol sterilizing 0.5min after with 0.1% mercuric chloride sterilizing 3min, to lucid asparagus seed asepsis sprouting
Action effect is preferable.
The sterilization effect of 3 different disposal of table
Experimental example 4：The screening of sprout suitable culture conditions
Lucid asparagus seed after sterilizing is inoculated in and adds the MS culture mediums of different plant growth regulating substances and (contains sucrose
30g·L-1, agar 7gL-1, pH 5.8) on, after 1d, 3d, 5d light culture grow white bud point after kind of a hole, then it is placed in
1000lx, 2000lx, 3000lx, 4000lx illumination cultivation, light application time 12hd-1, not add Plant growth regulators
Matter, without photo-irradiation treatment be control (CK), cultivation temperature is (25 ± 2) DEG C, and bud ratio is counted after 15d, is observed and recorded after 20d
Sprout growth potential.The result shows that lucid asparagus seed is grown by 3d light cultures after 2000lx illumination cultivations, its budding and sprout
Preferably (table 4)；6-BA 2.0mgL are added in culture medium-1And GA30.5mg·L-1, that is, with MS+6-BA 2mgL-1+
GA30.5mg·L-1+ sucrose 30gL-1+ agar 7gL-1For culture medium (pH 5.8), it sprouts and the optimal (table of sprout growth
5)。
The influence that 4 illumination condition of table grows lucid asparagus seed germination and sprout
The influence that the different plant growth regulating substance combinations of table 5 grow seed germination and sprout
Explant material of the lucid asparagus stalwartness sprout obtained using this method culture as lucid asparagus tissue-culturing rapid propagation, is easy to
The proliferative induction of its callus and being differentiated to form for test tube seedling.
Compared with prior art, the present invention is using lucid asparagus mature seed as material, after it is soaked with the concentrated sulfuric acid and warm water
It is placed on the minimal medium for adding certain plant growth regulating substance, is sprouted under suitable Thermo-Photo sensitive and obtain healthy and strong sprout,
Using this sprout segment as the explant of tissue cultures, so as to be successfully established the clone of lucid asparagus tissue-culturing rapid propagation.Namely
Say, the sterile sprout from lucid asparagus seed is the preferable explant material of its tissue-culturing rapid propagation.This method can be carried effectively
Suitable explant material needed for for lucid asparagus tissue-culturing rapid propagation, so as to be successfully established lucid asparagus tissue-cultured seedling large-scale production technology
Provide safeguard.
Embodiment：
Invention is further described with reference to embodiment, but is not intended as the foundation limited the present invention.
Embodiment 1.
A kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation, specifically includes following steps：
1. the lucid asparagus seed of mature and plump is placed in the concentrated sulfuric acid that mass fraction is 98% after pre-processing 15min, A is obtained
Product；
2. A product are transferred in 25~55 DEG C of warm water the 5h that soaks seed, B product are obtained；
3. the intact seed of step B product mesosperms is selected successively with 75% alcohol sterilizing 1min, 0.1% mercuric chloride sterilizing 3min,
Sterile water cleans seed, obtains C product, spare；
4. C product are inoculated on MS culture mediums, first dark culturing 3d, move to 1000 after seed grows white bud point~
Cultivated under 4000lx intensities of illumination, light application time 12hd-1, cultivation temperature sprouts culture for (25 ± 2) DEG C, until growing strong
Strong lucid asparagus sprout, in this, as the suitable explant material of lucid asparagus tissue-culturing rapid propagation；
The MS culture mediums content is：6-BA2.0mg·L-1、GA30.5mg·L-1, sucrose 30gL-1With 5.8 fine jades of pH
Fat 7gL-1。
Embodiment 2.
A kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation, specifically includes following steps：
1. the lucid asparagus seed of mature and plump is placed in the concentrated sulfuric acid that mass fraction is 98% after pre-processing 20min, A is obtained
Product；
2. A product are transferred in 25~55 DEG C of warm water the 8h that soaks seed, B product are obtained；
3. the intact seed of step B product mesosperms is selected successively with 75% alcohol sterilizing 2min, 0.1% mercuric chloride sterilizing 5min,
Sterile water cleans seed, obtains C product, spare；
4. C product are inoculated on MS culture mediums, first dark culturing 5d, move to 1000 after seed grows white bud point~
Cultivated under 4000lx intensities of illumination, light application time 12hd-1, cultivation temperature sprouts culture for (25 ± 2) DEG C, until growing strong
Strong lucid asparagus sprout, in this, as the suitable explant material of lucid asparagus tissue-culturing rapid propagation；
The MS culture mediums content is：6-BA 2.5mg·L-1、GA31mg·L-1, sucrose 35gL-1With 5.8 agar of pH
10g·L-1。
Embodiment 3.
A kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation, specifically includes following steps：
1. the lucid asparagus seed of mature and plump is placed in the concentrated sulfuric acid that mass fraction is 98% after pre-processing 2min, A is obtained
Product；
2. A product are transferred in 25~55 DEG C of warm water the 2h that soaks seed, B product are obtained；
3. select the intact seed of step B product mesosperms to be sterilized with 75% alcohol sterilizing 0.5min, 0.1% mercuric chloride successively
1min, sterile water clean seed, obtain C product, spare；
4. C product are inoculated on MS culture mediums, first dark culturing 1d, move to 1000 after seed grows white bud point~
Cultivated under 4000lx intensities of illumination, light application time 12hd-1, cultivation temperature sprouts culture for (25 ± 2) DEG C, until growing strong
Strong lucid asparagus sprout, in this, as the suitable explant material of lucid asparagus tissue-culturing rapid propagation；
The MS culture mediums content is：6-BA 1.5mg·L-1, sucrose 25gL-1With 5.8 agar 4gL of pH-1。
Claims (5)
- A kind of 1. method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation, it is characterised in that：Using lucid asparagus mature seed as material Material, carries out immersion treatment with the concentrated sulfuric acid and warm water respectively, is placed on the minimal medium of addition plant growth regulating substance Sprouted under suitable Thermo-Photo sensitive, until the lucid asparagus sprout of stalwartness is grown, in this, as the suitable of lucid asparagus tissue-culturing rapid propagation Explant material.
- 2. the method for the suitable explant of lucid asparagus tissue-culturing rapid propagation is obtained as claimed in claim 1, it is characterised in that：Specifically include Following steps：1. the lucid asparagus seed of mature and plump is placed in the concentrated sulfuric acid after pre-processing 2~20 min, A product are obtained；2. A product are transferred to 2~8 h of seed soaking in 25~55 DEG C of warm water, B product are obtained；3. the intact seed of step B product mesosperms is selected successively with 75% alcohol sterilizing, 0.5~2 min, 0.1% mercuric chloride sterilizing 1~5 Min, sterile water clean seed, obtain C product, spare；4. C product, which are inoculated on MS culture mediums, carries out sprouting culture, until the lucid asparagus sprout of stalwartness is grown, in this, as Tianmen The suitable explant material of winter tissue-culturing rapid propagation.
- 3. the method for the suitable explant of lucid asparagus tissue-culturing rapid propagation is obtained as claimed in claim 2, it is characterised in that：The MS trainings Supporting base content is：1.5~2.5 mgL of 6-BA-1 、GA30~1 mgL-1, sucrose 25-35 gL-1With 5.8 fine jades of pH Fat 4-10 gL-1。
- 4. the method for the suitable explant of lucid asparagus tissue-culturing rapid propagation is obtained as claimed in claim 3, it is characterised in that：The MS trainings Supporting base content is：6-BA 2.0 mg·L-1 、GA3 0.5mg·L-1, 30 gL of sucrose-1With 5.8 agar of pH, 7 gL-1。
- 5. the method for the suitable explant of lucid asparagus tissue-culturing rapid propagation is obtained as claimed in claim 2, it is characterised in that：Described sprouts Hair culture, until growing the lucid asparagus sprout of stalwartness；It is 1~5 d of C product elder generation dark culturing, is moved after seed grows white bud point Cultivated under to 1000~4000 lx intensities of illumination, light application time is 12 hd-1, cultivation temperature is（25±2）DEG C, until growing Lucid asparagus sprout.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548428A (en) * | 2019-01-09 | 2019-04-02 | 三亚市南繁科学技术研究院 | A kind of sterile germination accelerating method of monkey-bread tree and vernalization culture medium |
| CN110521607A (en) * | 2019-10-15 | 2019-12-03 | 玉林师范学院 | A kind of asparagus fern inducing clumping bud method |
| CN112273231A (en) * | 2020-10-30 | 2021-01-29 | 贵州大学 | Method for inducing proliferation of asparagus cochinchinensis cluster buds and plant regeneration |
| CN116458427A (en) * | 2023-04-14 | 2023-07-21 | 广西壮族自治区农业科学院 | Method for producing root-knot potatoes in asparagus bottle |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210663A (en) * | 1998-09-08 | 1999-03-17 | 中国科学院昆明植物研究所 | Seed sprouting and quick propagating technology of Yunnan Rhizoma Paridis |
| US6905876B2 (en) * | 2001-11-16 | 2005-06-14 | Council Of Scientific And Industrial Research | Methods and compositions for in vitro germination and propagation of polygonatum cirrhifolium royle |
| CN101451119A (en) * | 2008-12-26 | 2009-06-10 | 南京师范大学 | Phytolacca americana seed quick-speed germination method and its application in aseptic seedlings culture |
| CN101946705A (en) * | 2010-08-18 | 2011-01-19 | 怀化学院 | Method for propagating cochinchnese asparagus root simply, efficiently and quickly |
| CN102172218A (en) * | 2011-01-26 | 2011-09-07 | 浙江大学 | Method for restoring activity of inactive lycoris seed |
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
| CN103858769A (en) * | 2014-04-03 | 2014-06-18 | 张旺凡 | Rapid rhizoma polygonati propagation technology method |
| CN103931502A (en) * | 2014-04-29 | 2014-07-23 | 中南民族大学 | Two-step seedling growing method for propagating paris polyphylla seeds |
| CN104304013A (en) * | 2014-10-14 | 2015-01-28 | 南京帝道农业科技有限公司 | Rapid propagation method for asparagus fern mutagenesis breeding |
| CN105052737A (en) * | 2015-07-17 | 2015-11-18 | 云南省农业科学院药用植物研究所 | Tissue culture method for culturing Polygonatum kingianum seeds into seedlings in one step |
| CN105230484A (en) * | 2015-09-25 | 2016-01-13 | 马龙县兴裕农业生物科技开发有限公司 | Rapid breeding method of rhizoma polygonati |
-
2017
- 2017-12-15 CN CN201711350938.6A patent/CN107980634A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210663A (en) * | 1998-09-08 | 1999-03-17 | 中国科学院昆明植物研究所 | Seed sprouting and quick propagating technology of Yunnan Rhizoma Paridis |
| US6905876B2 (en) * | 2001-11-16 | 2005-06-14 | Council Of Scientific And Industrial Research | Methods and compositions for in vitro germination and propagation of polygonatum cirrhifolium royle |
| CN101451119A (en) * | 2008-12-26 | 2009-06-10 | 南京师范大学 | Phytolacca americana seed quick-speed germination method and its application in aseptic seedlings culture |
| CN101946705A (en) * | 2010-08-18 | 2011-01-19 | 怀化学院 | Method for propagating cochinchnese asparagus root simply, efficiently and quickly |
| CN102172218A (en) * | 2011-01-26 | 2011-09-07 | 浙江大学 | Method for restoring activity of inactive lycoris seed |
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
| CN103858769A (en) * | 2014-04-03 | 2014-06-18 | 张旺凡 | Rapid rhizoma polygonati propagation technology method |
| CN103931502A (en) * | 2014-04-29 | 2014-07-23 | 中南民族大学 | Two-step seedling growing method for propagating paris polyphylla seeds |
| CN104304013A (en) * | 2014-10-14 | 2015-01-28 | 南京帝道农业科技有限公司 | Rapid propagation method for asparagus fern mutagenesis breeding |
| CN105052737A (en) * | 2015-07-17 | 2015-11-18 | 云南省农业科学院药用植物研究所 | Tissue culture method for culturing Polygonatum kingianum seeds into seedlings in one step |
| CN105230484A (en) * | 2015-09-25 | 2016-01-13 | 马龙县兴裕农业生物科技开发有限公司 | Rapid breeding method of rhizoma polygonati |
Non-Patent Citations (8)
| Title |
|---|
| S.GUPTA: "Improvement of seed germination", 《JOURNAL OF HERBS, SPICES & MEDICINAL PLANTS》 * |
| 刘玉艳等: "天门冬种子萌发特性研究 ", 《种子》 * |
| 曲春香等: "雉隐天冬的根培养 ", 《牡丹江师范学院学报(自然科学版)》 * |
| 樊君等: "GA对伊贝母休眠外植体诱导愈伤组织的作用 ", 《中药材》 * |
| 葛锦华等: "天冬愈伤诱导培养基的筛选 ", 《贵州农业科学》 * |
| 韩晶宏等: "6-BA和GA_3浸种对麦冬种子萌发及幼苗生长的影响 ", 《江苏农业科学》 * |
| 马怡迪等: "巨球百合无菌播种影响因素的研究 ", 《湖南农业大学学报(自然科学版)》 * |
| 黄宗华: "七叶一枝花种子萌发和组织培养 ", 《安徽农业科学》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548428A (en) * | 2019-01-09 | 2019-04-02 | 三亚市南繁科学技术研究院 | A kind of sterile germination accelerating method of monkey-bread tree and vernalization culture medium |
| CN110521607A (en) * | 2019-10-15 | 2019-12-03 | 玉林师范学院 | A kind of asparagus fern inducing clumping bud method |
| CN110521607B (en) * | 2019-10-15 | 2022-07-15 | 玉林师范学院 | Asparagus fern cluster bud induction method |
| CN112273231A (en) * | 2020-10-30 | 2021-01-29 | 贵州大学 | Method for inducing proliferation of asparagus cochinchinensis cluster buds and plant regeneration |
| CN116458427A (en) * | 2023-04-14 | 2023-07-21 | 广西壮族自治区农业科学院 | Method for producing root-knot potatoes in asparagus bottle |
| CN116458427B (en) * | 2023-04-14 | 2024-01-26 | 广西壮族自治区农业科学院 | Method for producing root-knot potatoes in asparagus bottle |
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Application publication date: 20180504 |