CN104304013A - Rapid propagation method for asparagus fern mutagenesis breeding - Google Patents
Rapid propagation method for asparagus fern mutagenesis breeding Download PDFInfo
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- CN104304013A CN104304013A CN201410540526.9A CN201410540526A CN104304013A CN 104304013 A CN104304013 A CN 104304013A CN 201410540526 A CN201410540526 A CN 201410540526A CN 104304013 A CN104304013 A CN 104304013A
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- asparagus fern
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Abstract
The invention discloses a rapid propagation method for asparagus fern mutagenesis breeding. The rapid propagation method comprises the following steps: seed disinfection, seed mutagenesis, cluster bud proliferation, rooting induction and the like. According to the method disclosed by the invention, metabolic pathways of cells are changed by processing through a mutagenic reagent so as to obtain high-yield strains with genetic stability.
Description
Technical field
The present invention relates to the quick-breeding method of asparagus fern tissue cultures, belong to field of plant growing technology.
Background technology
Asparagus fern, Liliaceae, climbs up by holding on to shape perennial herb.In being born in dark and damp hill woods limit, thick grass or shrub, also there is cultivation.Be distributed in East China, Central-South, the southwestern and ground such as Hebei, Shanxi, Shaanxi, Gansu, Taiwan.Cold in nature, taste is sweet, micro-hardship.Containing multiple spiral steroid glycoside compound asparagus fern glucoside IV ~ VII, nearly 20 seed amino acids such as asparagine, citrulling, serine, and oligosaccharide I ~ VII; And containing 5-methoxy-methylfurfural, there is replenishing the vital essence and removing heat, effect of moistening lung nourshing kidney.For fever due to yin deficiency, lung carbuncle, quench one's thirst, the effect of hemocytes increasing, enhancing reticuloendothelial system phagocytic function and prolongation antibody life period.Main employing seed and division propagation at present, there is not been reported for tissue culture method.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for quickly breeding of asparagus fern, by the process of the method by mutagen, changes the metabolic pathway of cell, obtains the superior strain of inheritance stability.
Technical problem to be solved by this invention is realized by following scheme:
Choose the seed that asparagus fern is full, 25min is soaked in the alcohol of 70%, the liquor natrii hypochloritis of 1% disinfects 18min, then clean with aseptic water washing, the asparagus fern seed of disinfecting is inoculated into not containing in the modified MS medium of any hormone, additional saccharose 45g/L, agar 6.5g/L, pH6.0, temperature 25 DEG C, nitrous acid process 10-30min, the aseptic seedling grown, cut away radicle and be inoculated into MT+3.0mg/L6-BA+0.5mg/L2, adventitious buds proliferation cultivation is carried out in 4-D medium, additional saccharose 30g/L, agar 6.5g/L, pH6.0, temperature 25 DEG C, intensity of illumination 35 μm of olm
-2s
-1, light application time 10h/d, the Multiple Buds after propagation three clump is inoculated in the root media of MT+NAA0.4mg/L+ active carbon 3g/L and carries out root induction, additional saccharose 20g/L, agar 5.5g/L, pH6.0, temperature 27 DEG C, intensity of illumination 65 μm of olm
-2s
-1, light application time 17h/d.
The asparagus fern regeneration rate adopting the present invention to prepare is high, and the cycle is short, and output is large, pollutes little, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Choose the seed that asparagus fern is full, 25min is soaked in the alcohol of 70%, the liquor natrii hypochloritis of 1% disinfects 18min, then clean with aseptic water washing, the asparagus fern seed of disinfecting is inoculated into not containing in the modified MS medium of any hormone, additional saccharose 45g/L, agar 6.5g/L, pH6.0, temperature 25 DEG C, nitrous acid process 10min, the aseptic seedling grown, cut away radicle and be inoculated into MT+3.0mg/L6-BA+0.5mg/L2, adventitious buds proliferation cultivation is carried out in 4-D medium, additional saccharose 30g/L, agar 6.5g/L, pH6.0, temperature 25 DEG C, intensity of illumination 35 μm of olm
-2s
-1, light application time 10h/d, the Multiple Buds after propagation three clump is inoculated in the root media of MT+NAA0.4mg/L+ active carbon 3g/L and carries out root induction, additional saccharose 20g/L, agar 5.5g/L, pH6.0, temperature 27 DEG C, intensity of illumination 65 μm of olm
-2s
-1, light application time 17h/d, mutagenesis survival rate 91%.
Embodiment 2
Choose the seed that asparagus fern is full, 25min is soaked in the alcohol of 70%, the liquor natrii hypochloritis of 1% disinfects 18min, then clean with aseptic water washing, the asparagus fern seed of disinfecting is inoculated into not containing in the modified MS medium of any hormone, additional saccharose 45g/L, agar 6.5g/L, pH6.0, temperature 25 DEG C, nitrous acid process 20min, the aseptic seedling grown, cut away radicle and be inoculated into MT+3.0mg/L6-BA+0.5mg/L2, adventitious buds proliferation cultivation is carried out in 4-D medium, additional saccharose 30g/L, agar 6.5g/L, pH6.0, temperature 25 DEG C, intensity of illumination 35 μm of olm
-2s
-1, light application time 10h/d, the Multiple Buds after propagation three clump is inoculated in the root media of MT+NAA0.4mg/L+ active carbon 3g/L and carries out root induction, additional saccharose 20g/L, agar 5.5g/L, pH6.0, temperature 27 DEG C, intensity of illumination 65 μm of olm
-2s
-1, light application time 17h/d, mutagenesis survival rate 92%.
Embodiment 3
Choose the seed that asparagus fern is full, 25min is soaked in the alcohol of 70%, the liquor natrii hypochloritis of 1% disinfects 18min, then clean with aseptic water washing, the asparagus fern seed of disinfecting is inoculated into not containing in the modified MS medium of any hormone, additional saccharose 45g/L, agar 6.5g/L, pH6.0, temperature 25 DEG C, nitrous acid process 30min, the aseptic seedling grown, cut away radicle and be inoculated into MT+3.0mg/L6-BA+0.5mg/L2, adventitious buds proliferation cultivation is carried out in 4-D medium, additional saccharose 30g/L, agar 6.5g/L, pH6.0, temperature 25 DEG C, intensity of illumination 35 μm of olm
-2s
-1, light application time 10h/d, the Multiple Buds after propagation three clump is inoculated in the root media of MT+NAA0.4mg/L+ active carbon 3g/L and carries out root induction, additional saccharose 20g/L, agar 5.5g/L, pH6.0, temperature 27 DEG C, intensity of illumination 65 μm of olm
-2s
-1, light application time 17h/d, mutagenesis survival rate 94%.
Claims (2)
1. a method for quickly breeding for asparagus fern mutation breeding, comprise the sterilization of seed, the mutagenesis of seed, the propagation of Multiple Buds, root induction, its key step is as follows:
(1) seed of asparagus fern is got, disinfection;
(2) getting the asparagus fern seed that step (1) disinfected is inoculated into not containing in the modified MS medium of any hormone, additional saccharose 45g/L, agar 6.5g/L, pH6.0, temperature 25 DEG C, nitrous acid process 10-30min;
(3) get the aseptic seedling that step (2) grows, cut away radicle and be inoculated into MT+3.0mg/L6-BA+0.5mg/L2, in 4-D medium, carry out adventitious buds proliferation cultivation, additional saccharose 30g/L, agar 6.5g/L, pH6.0, temperature 25 DEG C, intensity of illumination 35 μm of olm
-2s
-1, light application time 10h/d;
(4) Multiple Buds three clump got after step (3) propagation is inoculated in the root media of MT+NAA0.4mg/L+ active carbon 3g/L and carries out root induction, additional saccharose 20g/L, agar 5.5g/L, pH6.0, temperature 27 DEG C, intensity of illumination 65 μm of olm
-2s
-1, light application time 17h/d.
2. according to the method for quickly breeding of a kind of asparagus fern mutation breeding according to claim 1, it is characterized in that: in step (1), the sterilization method of asparagus fern seed is choose the full seed of asparagus fern, 25min is soaked in the alcohol of 70%, the liquor natrii hypochloritis of 1% disinfects 18min, then clean with aseptic water washing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410540526.9A CN104304013A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for asparagus fern mutagenesis breeding |
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| CN201410540526.9A CN104304013A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for asparagus fern mutagenesis breeding |
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| CN104304013A true CN104304013A (en) | 2015-01-28 |
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| CN201410540526.9A Pending CN104304013A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for asparagus fern mutagenesis breeding |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145359A (en) * | 2015-09-15 | 2015-12-16 | 广西壮族自治区药用植物园 | Tissue culture and rapid propagation method for asparagus filicinus |
| CN106472309A (en) * | 2016-10-09 | 2017-03-08 | 韦波 | A kind of Radix Asparagi tissue cultured seedling culture medium improving asparagine |
| CN107980634A (en) * | 2017-12-15 | 2018-05-04 | 贵州大学 | A kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation |
| CN110521607A (en) * | 2019-10-15 | 2019-12-03 | 玉林师范学院 | A kind of asparagus fern inducing clumping bud method |
-
2014
- 2014-10-14 CN CN201410540526.9A patent/CN104304013A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145359A (en) * | 2015-09-15 | 2015-12-16 | 广西壮族自治区药用植物园 | Tissue culture and rapid propagation method for asparagus filicinus |
| CN106472309A (en) * | 2016-10-09 | 2017-03-08 | 韦波 | A kind of Radix Asparagi tissue cultured seedling culture medium improving asparagine |
| CN107980634A (en) * | 2017-12-15 | 2018-05-04 | 贵州大学 | A kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation |
| CN110521607A (en) * | 2019-10-15 | 2019-12-03 | 玉林师范学院 | A kind of asparagus fern inducing clumping bud method |
| CN110521607B (en) * | 2019-10-15 | 2022-07-15 | 玉林师范学院 | Asparagus fern cluster bud induction method |
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Application publication date: 20150128 |
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