CN106472309A - A kind of Radix Asparagi tissue cultured seedling culture medium improving asparagine - Google Patents
A kind of Radix Asparagi tissue cultured seedling culture medium improving asparagine Download PDFInfo
- Publication number
- CN106472309A CN106472309A CN201610877881.4A CN201610877881A CN106472309A CN 106472309 A CN106472309 A CN 106472309A CN 201610877881 A CN201610877881 A CN 201610877881A CN 106472309 A CN106472309 A CN 106472309A
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- CN
- China
- Prior art keywords
- salt
- asparagine
- radix asparagi
- culture medium
- seedling culture
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- UFWIBTONFRDIAS-UHFFFAOYSA-N naphthalene-acid Natural products C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 1
- 125000001624 naphthyl group Chemical group 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of Radix Asparagi tissue cultured seedling culture medium improving asparagine, including following raw material：Fruice, potassium salt, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese salt, mantoquita, iron salt, vitamin, aminoacid, indolebutyric acid, gibberellins, 6 benzyl gland fat purines, naphthalene acetic acid, kinetins, conditioner, cosolvent, dispersant, compatilizer, stabilizer, antibacterial, water.The seedling quality being obtained using the Radix Asparagi tissue cultured seedling culture medium tissue culture Radix Asparagi of the raising asparagine of the present invention is good, and survival rate is high and effective ingredient asparagine is high.
Description
Technical field
The invention belongs to Radix Asparagi planting technology field is and in particular to a kind of Radix Asparagi tissue cultured seedling of raising asparagine is cultivated
Base.
Background technology
Radix Asparagi is a kind of conventional Chinese medicinal material, has YIN nourishing and the production of body fluid promoting, effect of moistening lung clears away heart-fire.In recent years, right on market
The demand of wild Radix Asparagi significantly increases, and causes wild Radix Asparagi to suffer the artificial cultivation technique of destructive therefore Radix Asparagi also progressively
Grow up.Because most Radix Asparagi is all to carry out scattered plantation by peasant in the market, kind is more miscellaneous, the having of Radix Asparagi
Effect composition asparagine content is difficult to be protected.
Content of the invention
The present invention provides a kind of Radix Asparagi tissue cultured seedling culture medium improving asparagine, how to solve existing tissue culture medium (TCM)
Improve the content of the principle active component asparagine of Radix Asparagi, the problems such as increasing economic efficiency.Raising Tianmen using the present invention
The seedling quality that obtained of Radix Asparagi tissue cultured seedling culture medium tissue culture Radix Asparagi of winter element is good, and survival rate is high and effective ingredient asparagine
High.
For solving above technical problem, the present invention employs the following technical solutions：
A kind of Radix Asparagi tissue cultured seedling culture medium improving asparagine, in units of weight portion, including following raw material：Fruice 122-
348 parts, potassium salt 2.3-3.7 part, microcosmic salt 1.4-3.4 part, nitrogen salt 1.2-2.3 part, calcium salt 0.7-1 part, magnesium salt 0.6-0.9 part, iodine
Salt 0.3-0.5 part, boron salt 0.2-0.4 part, molybdenum salt 0.2-0.3 part, zinc salt 0.3-0.4 part, manganese salt 0.1-0.3 part, mantoquita 0.1-
0.2 part, iron salt 0.1-0.2 part, vitamin 0.09-0.25 part, aminoacid 0.08-0.2 part, indolebutyric acid 0.05-0.1 part, red
Mycin 0.06-0.09 part, 6- benzyl gland fat purine 0.04-0.08 part, naphthalene acetic acid 0.06-0.09 part, kinetins 0.03-0.06 part,
Conditioner 0.17-0.22 part, cosolvent 1.4-2.8 part, dispersant 1.2-2.3 part, compatilizer 1-1.9 part, stabilizer 0.9-1.8
Part, antibacterial 1.5-2.8 part, water 2500-3500 part；
Described fruice is Fructus Citri Limoniae juice；
Described potassium salt is potassium nitrate；
Described microcosmic salt is calcium phosphate；
Described nitrogen salt is ammonium carbonate；
Described calcium salt is sulphuric acid Asia calcium；
Described magnesium salt is sulphuric acid Asia magnesium；
Described iodine salt is potassium iodate；
Described boron salt is potassium borate；
Described molybdenum salt is sodium molybdate；
Described zinc salt is zinc chloride；
Described manganese salt is manganous sulphate；
Described mantoquita is copper sulfate；
Described iron salt is iron chloride；
Described vitamin is Vitamin E；
Described aminoacid is proline；
The described Radix Asparagi tissue cultured seedling culture medium improving asparagine, its preparation method comprises the following steps：
S1：Fruit is blended after Fructus Citri Limoniae peeling with pulper, gained was squeezed the juice 3000-5000 mesh sieve, prepared fruice；
S2：By the prepared fruice of step S1, potassium salt, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese
Salt, mantoquita, iron salt, vitamin, aminoacid, indolebutyric acid, gibberellins, 6- benzyl gland fat purine, naphthalene acetic acid, kinetins, cosolvent,
Dispersant, compatilizer, antibacterial, water mixing, are 95-135W in microwave power, temperature is 64-70 DEG C, and rotating speed is 200-400r/
28-52min, prepared mixed liquor A is stirred under min；
S3：Add conditioner, stabilizer in the mixed liquor A being obtained to step S2, be 47-53 DEG C in temperature, rotating speed is 100-
14-24min, prepared mixed liquid B is stirred under 300r/min；
S4：The pH value of the mixed liquid B that step S3 is obtained is adjusted to 5.7-6.4, is then 134-136 DEG C in temperature, pressure is
Under 210-230KPa, sterilize 4-6min, and the Radix Asparagi tissue cultured seedling culture medium that improve asparagine is obtained.
Further, described conditioner is humic acid.
Further, described cosolvent is Polyvinylpyrrolidone.
Further, described dispersant is in units of weight portion, including following raw material：Potassium carboxymethylcellulose 3-7 part, fine jade
Fat 2-4 part, modified starch 3-5 part, sodium polyacrylate 2-3 part, sodium alginate 1-2 part.
Further, described compatilizer is Dupont EEA2112AC.
Further, described stabilizer is carrageenan.
Further, described antibacterial is methyl-furfuryl fumarate.
The effect of medium component of the present invention is as follows：
The accumulation being added with beneficial to polyoses content of fruice.
Apple polyphenol, as a kind of antioxidant, can make cell growth slow it is also possible to promote accumulation of flavonoids.
The nutritive effect of potassium element can improve photosynthetic intensity, promotes the formation of starch and sugar in crop body, increases
Pretend the resistance of thing and resistances against diseases absorb to nitrogen moreover it is possible to improve crop.
Phosphorus is to form the indispensable elements such as Nuclear extract, lecithin.P elements can accelerate cell division, promotes root
System and overground part accelerate growth, promote bud differentiation, ripe ahead of time, improve fruit quality.
Nitrogen is the essential element that protein is constituted, and protein is the base substance in cellular plasm composition.Nitrogen energy
Promote protein and chlorophyllous formation, make dark green leaf color, leaf area increases, promote the assimilation of carbon, be conducive to yield to increase, product
Matter is improved.
The growth lacking calcium plant meristem is slack-off, and, just in growing point and spire, when serious, leaf deforms for calcium deficiency and disease
And chlorosis, at the edge of leaf, necrotic spot occurs.
Magnesium is chlorophyllous constituent.If lacking magnesium, blade loses green it is impossible to manufacture nutrient substance.
Iodine is the indispensable element of plant growing, is described as biological element, wisdom element.
Boron compares in plant and concentrates in stem apex, the tip of a root, blade and floral organ, and the content in dicotyledon is usually
Higher than monocotyledon.Boron can improve the nitrogenase activity of legume root nodule bacteria, increases amount of nitrogen fixation, during boron deficiency, root stays not to be sent out
Reach, affect amount of nitrogen fixation.Boron can also strengthen crop anti-adversity.Molybdenum is nitrogen-fixing microorganism, particularly the root nodule with legume symbiosis
Necessary during the fixing air nitrogen of bacterium.Leaf photosynthesis intensity can be promoted simultaneously again.During scarce molybdenum, plant is short and small, between blade arteries and veins
Chlorosis, leaf margin is dried-up, inward curl, becomes wilting state.
Zinc can promote crop to carry out photosynthesis, and it is the constituent of multiple enzymes, and during zinc deficiency, stagnating occurs in crop growth.
Manganese is contained, manganese can promote germination and the growth of seedling period, affect the photosynthesis of crop, exhale during manganese deficiency in plant chloroplast
The accumulation in vivo of suction effect, nitrate nitrogen.Show as chlorosis yellow between blade arteries and veins, have brown spot.Copper participates in the photosynthetic of plant
Effect, Repiration, nitrogen metabolism, the resistance of enhancing plant.During scarce copper, plant strain growth is thin and weak, and young leaves turns to be yellow, wilting withered, blade tip
Curling is turned white, and has necrotic spot.Ferrum is that chlorophyll formation is indispensable, is difficult to transfer in plant body, so blade " chlorosis
Disease " is the performance of plant iron deficiency, and this chlorosis shows on young leaflet tablet first.Vitamin taking root effectively to plant
Should, plant establishment can be promoted.
Aminoacid is nutrient substance necessary to plant growing, due to the characteristic of aminoacid itself, special to plant growing
It is the facilitation that photosynthesis have uniqueness, it can increase chlorophyll content of plant, improves the activity of enzyme, promotes titanium dioxide
The infiltration of carbon, makes photosynthesis more vigorous, to improving crop quality, increases Vc and the content of sugar suffers from important function.
Indolebutyric acid is mainly used in rooting of cuttings, the formation of inducible root substance, promotes cell differentiation and division, is conducive to
New root generates the differentiation with fibrovascular system, promotes the formation of cutting adventitious root.
Gibberellins, are the class phytohormone being widely present.Its chemical constitution belongs to Diterpeneses acid, is derived by four ring skeletons
And obtain, the growth of leaf and bud can be stimulated.
6-benzyladenine, Chinese nickname be 2- benayl aminopurine, N- benzyl adenosine, n-butyl acrylate, white or
Off-white color crystal, is insoluble in water, is slightly soluble in ethanol, stable in acid, alkali, belongs to broad spectrum activity plant growth regulator, can promote to plant
Thing cell growth, the degraded of suppression plant chlorophyll, improves the content of aminoacid, Delaying Leaf-Senescence etc..
Naphthalene acetic acid (1-Naphthaleneacetic acid), abbreviation NAA, is a kind of organic compound, is a kind of readily soluble
In the colorless solid of organic solvent, its structure is that No. 1 position of naphthalene is replaced with carboxymethyl, and it is a Plant Hormone growth
Element, is usually used in root of hair powder or the rooting agent of commercialization, uses when plant uses cuttage breeding.It can also be used for plant tissue
Culture.
Kinetins are a kind of non-natural basic elements of cell division, and chemical name is 6- glycosyl amidopurin (or N6- furfuryl
Adenine).Water insoluble, it is dissolved in strong acid, alkali and glacial acetic acid；In addition to having and promoting fissional effect, also have and delay
Excised leaf and cutting flower withering, induced bud differentiation and the effect developing and increasing stomatal aperture.
Conditioner can promote growth and the beneficial microorganism activity of root system.
Cosolvent increases culture medium dissolubility in a solvent.
Dispersant can prevent from flocculating, and prevents from settling.
Compatilizer refers to by means of intermolecular bonding force, promotes incompatible two kinds of polymer to combine in one, and then
Obtain the auxiliary agent of stable blend.
Stabilizer can increase the stability of solution, colloid, solid, mixture.
Antibacterial is can to make certain micro-organisms within a certain period of time（Antibacterial, funguses, yeast, algae and virus etc.）
Growth or breeding be maintained at necessary chemical substance below horizontal.
The invention has the advantages that：
（1）The seedling quality being obtained using the Radix Asparagi tissue cultured seedling culture medium tissue culture Radix Asparagi of the raising asparagine of the present invention is good,
Survival rate is high and effective ingredient asparagine is high；
（2）Using the Radix Asparagi culture medium preparation method of the present invention, disinfecting time can be shortened, improve sterilization effect reliability and product
Industry production efficiency, reduces production cost；
（3）The Radix Asparagi culture medium preparation method of the present invention, simple to operate, it is easy to accomplish industrialization, standardization and standardized production.
Specific embodiment
For ease of more fully understanding the present invention, it is illustrated by following examples, these embodiments belong to the present invention's
Protection domain, but do not limit the scope of the invention.
In an embodiment, the described Radix Asparagi tissue cultured seedling culture medium improving asparagine, in units of weight portion, including following
Raw material：Fruice 122-348 part, potassium salt 2.3-3.7 part, microcosmic salt 1.4-3.4 part, nitrogen salt 1.2-2.3 part, calcium salt 0.7-1 part, magnesium
Salt 0.6-0.9 part, iodine salt 0.3-0.5 part, boron salt 0.2-0.4 part, molybdenum salt 0.2-0.3 part, zinc salt 0.3-0.4 part, manganese salt 0.1-
0.3 part, mantoquita 0.1-0.2 part, iron salt 0.1-0.2 part, vitamin 0.09-0.25 part, aminoacid 0.08-0.2 part, indolebutyric acid
0.05-0.1 part, gibberellins 0.06-0.09 part, 6- benzyl gland fat purine 0.04-0.08 part, naphthalene acetic acid 0.06-0.09 part, kinetins
0.03-0.06 part, conditioner 0.17-0.22 part, cosolvent 1.4-2.8 part, dispersant 1.2-2.3 part, compatilizer 1-1.9 part,
Stabilizer 0.9-1.8 part, antibacterial 1.5-2.8 part, water 2500-3500 part；
Described fruice is Fructus Citri Limoniae juice；
Described potassium salt is potassium nitrate；
Described microcosmic salt is calcium phosphate；
Described nitrogen salt is ammonium carbonate；
Described calcium salt is sulphuric acid Asia calcium；
Described magnesium salt is sulphuric acid Asia magnesium；
Described iodine salt is potassium iodate；
Described boron salt is potassium borate；
Described molybdenum salt is sodium molybdate；
Described zinc salt is zinc chloride；
Described manganese salt is manganous sulphate；
Described mantoquita is copper sulfate；
Described iron salt is iron chloride；
Described vitamin is Vitamin E；
Described aminoacid is proline；
Described conditioner is humic acid；
Described cosolvent is Polyvinylpyrrolidone；
Described dispersant in units of weight portion, including following raw material：Potassium carboxymethylcellulose 3-7 part, agar 2-4 part, modification
Starch 3-5 part, sodium polyacrylate 2-3 part, sodium alginate 1-2 part；
Described compatilizer is Dupont EEA2112AC；
Described stabilizer is carrageenan；
Described antibacterial is methyl-furfuryl fumarate；
The described Radix Asparagi tissue cultured seedling culture medium improving asparagine, its preparation method comprises the following steps：
S1：Fruit is blended after Fructus Citri Limoniae peeling with pulper, gained was squeezed the juice 3000-5000 mesh sieve, prepared fruice；
S2：By the prepared fruice of step S1, potassium salt, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese
Salt, mantoquita, iron salt, vitamin, aminoacid, indolebutyric acid, gibberellins, 6- benzyl gland fat purine, naphthalene acetic acid, kinetins, cosolvent,
Dispersant, compatilizer, antibacterial, water mixing, are 95-135W in microwave power, temperature is 64-70 DEG C, and rotating speed is 200-400r/
28-52min, prepared mixed liquor A is stirred under min；
S3：Add conditioner, stabilizer in the mixed liquor A being obtained to step S2, be 47-53 DEG C in temperature, rotating speed is 100-
14-24min, prepared mixed liquid B is stirred under 300r/min；
S4：The pH value of the mixed liquid B that step S3 is obtained is adjusted to 5.7-6.4, is then 134-136 DEG C in temperature, pressure is
Under 210-230KPa, sterilize 4-6min, and the Radix Asparagi tissue cultured seedling culture medium that improve asparagine is obtained.
Embodiment 1
A kind of Radix Asparagi tissue cultured seedling culture medium improving asparagine, in units of weight portion, including following raw material：Fruice 250
Part, 3 parts of potassium salt, 2.5 parts of microcosmic salt, 1.8 parts of nitrogen salt, 0.9 part of calcium salt, 0.8 part of magnesium salt, 0.4 part of iodine salt, 0.3 part of boron salt, molybdenum salt
0.2 part, 0.3 part of zinc salt, 0.2 part of manganese salt, 0.1 part of mantoquita, 0.1 part of iron salt, 0.13 part of vitamin, 0.14 part of aminoacid, indole
0.08 part of butanoic acid, 0.08 part of gibberellins, 0.07 part of 6- benzyl gland fat purine, 0.08 part of naphthalene acetic acid, 0.05 part of kinetins, conditioner
0.15 part, 2.2 parts of cosolvent, 1.8 parts of dispersant, 1.5 parts of compatilizer, 1.2 parts of stabilizer, 2.2 parts of antibacterial, 3000 parts of water；
Described fruice is Fructus Citri Limoniae juice；
Described potassium salt is potassium nitrate；
Described microcosmic salt is calcium phosphate；
Described nitrogen salt is ammonium carbonate；
Described calcium salt is sulphuric acid Asia calcium；
Described magnesium salt is sulphuric acid Asia magnesium；
Described iodine salt is potassium iodate；
Described boron salt is potassium borate；
Described molybdenum salt is sodium molybdate；
Described zinc salt is zinc chloride；
Described manganese salt is manganous sulphate；
Described mantoquita is copper sulfate；
Described iron salt is iron chloride；
Described vitamin is Vitamin E；
Described aminoacid is proline；
Described conditioner is humic acid；
Described cosolvent is Polyvinylpyrrolidone；
Described dispersant in units of weight portion, including following raw material：6 parts of potassium carboxymethylcellulose, 3 parts of agar, modified starch 4
Part, 2 parts of sodium polyacrylate, 1 part of sodium alginate；
Described compatilizer is Dupont EEA2112AC；
Described stabilizer is carrageenan；
Described antibacterial is methyl-furfuryl fumarate；
The described Radix Asparagi tissue cultured seedling culture medium improving asparagine, its preparation method comprises the following steps：
S1：Fruit is blended after Fructus Citri Limoniae peeling with pulper, gained was squeezed the juice 4000 mesh sieves, prepared fruice；
S2：By the prepared fruice of step S1, potassium salt, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese
Salt, mantoquita, iron salt, vitamin, aminoacid, indolebutyric acid, gibberellins, 6- benzyl gland fat purine, naphthalene acetic acid, kinetins, cosolvent,
Dispersant, compatilizer, antibacterial, water mixing, are 120W in microwave power, temperature is 67 DEG C, and rotating speed is to stir under 300r/min
40min, prepared mixed liquor A；
S3：Add conditioner, stabilizer in the mixed liquor A being obtained to step S2, be 49 DEG C in temperature, rotating speed is under 200r/min
Stirring 18min, prepared mixed liquid B；
S4：The pH value of the mixed liquid B that step S3 is obtained is adjusted to 6, is then 135 DEG C in temperature, and pressure is under 220KPa, goes out
Bacterium 5min, is obtained the Radix Asparagi tissue cultured seedling culture medium improving asparagine.
Embodiment 2
A kind of Radix Asparagi tissue cultured seedling culture medium improving asparagine, in units of weight portion, including following raw material：Fruice 122
Part, 2.3 parts of potassium salt, 1.4 parts of microcosmic salt, 1.2 parts of nitrogen salt, 0.7 part of calcium salt, 0.6 part of magnesium salt, 0.3 part of iodine salt, 0.2 part of boron salt, molybdenum salt
0.2 part, 0.3 part of zinc salt, 0.1 part of manganese salt, 0.1 part of mantoquita, 0.1 part of iron salt, 0.09 part of vitamin, 0.08 part of aminoacid, indole
0.05 part of butanoic acid, 0.06 part of gibberellins, 0.04 part of 6- benzyl gland fat purine, 0.06 part of naphthalene acetic acid, 0.03 part of kinetins, conditioner
0.17 part, 1.4 parts of cosolvent, 1.2 parts of dispersant, 1 part of compatilizer, 0.9 part of stabilizer, 1.5 parts of antibacterial, 2500 parts of water；
Described fruice is Fructus Citri Limoniae juice；
Described potassium salt is potassium nitrate；
Described microcosmic salt is calcium phosphate；
Described nitrogen salt is ammonium carbonate；
Described calcium salt is sulphuric acid Asia calcium；
Described magnesium salt is sulphuric acid Asia magnesium；
Described iodine salt is potassium iodate；
Described boron salt is potassium borate；
Described molybdenum salt is sodium molybdate；
Described zinc salt is zinc chloride；
Described manganese salt is manganous sulphate；
Described mantoquita is copper sulfate；
Described iron salt is iron chloride；
Described vitamin is Vitamin E；
Described aminoacid is proline；
Described conditioner is humic acid；
Described cosolvent is Polyvinylpyrrolidone；
Described dispersant in units of weight portion, including following raw material：3 parts of potassium carboxymethylcellulose, 2 parts of agar, modified starch 3
Part, 2 parts of sodium polyacrylate, 1 part of sodium alginate；
Described compatilizer is Dupont EEA2112AC；
Described stabilizer is carrageenan；
Described antibacterial is methyl-furfuryl fumarate；
The described Radix Asparagi tissue cultured seedling culture medium improving asparagine, its preparation method comprises the following steps：
S1：Fruit is blended after Fructus Citri Limoniae peeling with pulper, gained was squeezed the juice 3000 mesh sieves, prepared fruice；
S2：By the prepared fruice of step S1, potassium salt, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese
Salt, mantoquita, iron salt, vitamin, aminoacid, indolebutyric acid, gibberellins, 6- benzyl gland fat purine, naphthalene acetic acid, kinetins, cosolvent,
Dispersant, compatilizer, antibacterial, water mixing, are 95W in microwave power, temperature is 64 DEG C, and rotating speed is to stir under 200r/min
52min, prepared mixed liquor A；
S3：Add conditioner, stabilizer in the mixed liquor A being obtained to step S2, be 47 DEG C in temperature, rotating speed is under 100r/min
Stirring 24min, prepared mixed liquid B；
S4：The pH value of the mixed liquid B that step S3 is obtained is adjusted to 5.7, is then 134 DEG C in temperature, and pressure is under 210KPa,
Sterilizing 6min, is obtained the Radix Asparagi tissue cultured seedling culture medium improving asparagine.
Embodiment 3
A kind of Radix Asparagi tissue cultured seedling culture medium improving asparagine, in units of weight portion, including following raw material：Fruice 348
Part, 3.7 parts of potassium salt, 3.4 parts of microcosmic salt, 2.3 parts of nitrogen salt, 1 part of calcium salt, 0.9 part of magnesium salt, 0.5 part of iodine salt, 0.4 part of boron salt, molybdenum salt
0.3 part, 0.4 part of zinc salt, 0.3 part of manganese salt, 0.2 part of mantoquita, 0.2 part of iron salt, 0.25 part of vitamin, 0.2 part of aminoacid, indole fourth
0.1 part of acid, 0.09 part of gibberellins, 0.08 part of 6- benzyl gland fat purine, 0.09 part of naphthalene acetic acid, 0.06 part of kinetins, conditioner 0.22
Part, 2.8 parts of cosolvent, 2.3 parts of dispersant, 1.9 parts of compatilizer, 1.8 parts of stabilizer, 2.8 parts of antibacterial, 3500 parts of water；
Described fruice is Fructus Citri Limoniae juice；
Described potassium salt is potassium nitrate；
Described microcosmic salt is calcium phosphate；
Described nitrogen salt is ammonium carbonate；
Described calcium salt is sulphuric acid Asia calcium；
Described magnesium salt is sulphuric acid Asia magnesium；
Described iodine salt is potassium iodate；
Described boron salt is potassium borate；
Described molybdenum salt is sodium molybdate；
Described zinc salt is zinc chloride；
Described manganese salt is manganous sulphate；
Described mantoquita is copper sulfate；
Described iron salt is iron chloride；
Described vitamin is Vitamin E；
Described aminoacid is proline；
Described conditioner is humic acid；
Described cosolvent is Polyvinylpyrrolidone；
Described dispersant in units of weight portion, including following raw material：7 parts of potassium carboxymethylcellulose, 4 parts of agar, modified starch 5
Part, 3 parts of sodium polyacrylate, 2 parts of sodium alginate；
Described compatilizer is Dupont EEA2112AC；
Described stabilizer is carrageenan；
Described antibacterial is methyl-furfuryl fumarate；
The described Radix Asparagi tissue cultured seedling culture medium improving asparagine, its preparation method comprises the following steps：
S1：Fruit is blended after Fructus Citri Limoniae peeling with pulper, gained was squeezed the juice 5000 mesh sieves, prepared fruice；
S2：By the prepared fruice of step S1, potassium salt, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese
Salt, mantoquita, iron salt, vitamin, aminoacid, indolebutyric acid, gibberellins, 6- benzyl gland fat purine, naphthalene acetic acid, kinetins, cosolvent,
Dispersant, compatilizer, antibacterial, water mixing, are 135W in microwave power, temperature is 70 DEG C, and rotating speed is to stir under 400r/min
28min, prepared mixed liquor A；
S3：Add conditioner, stabilizer in the mixed liquor A being obtained to step S2, be 53 DEG C in temperature, rotating speed is under 300r/min
Stirring 14min, prepared mixed liquid B；
S4：The pH value of the mixed liquid B that step S3 is obtained is adjusted to 6.4, is then 136 DEG C in temperature, and pressure is under 230KPa,
Sterilizing 4min, is obtained the Radix Asparagi tissue cultured seedling culture medium improving asparagine.
Radix Asparagi tissue cultured seedling culture medium and traditional Radix Asparagi group improving asparagine using the raising asparagine of the present invention
Seedlings cultivating culture medium（Matched group）Tissue culture Radix Asparagi Seedling, condition of tissue culture is basically identical, each embodiment or 100 plants of matched group tissue culture, knot
Really as shown in the table.
As seen from table, the kind being obtained using the Radix Asparagi tissue cultured seedling culture medium tissue culture Radix Asparagi of the raising asparagine of the present invention
Seedling quality is good, and survival rate is high and effective ingredient asparagine is high.
Above content it cannot be assumed that the present invention be embodied as be confined to these explanations, the affiliated technology neck for the present invention
For the those of ordinary skill in domain, without departing from the inventive concept of the premise, some simple deduction or replace can also be made,
The scope of patent protection that the present invention is determined all should be considered as belonging to by the claims submitted to.
Claims (7)
1. a kind of Radix Asparagi tissue cultured seedling culture medium improving asparagine it is characterised in that in units of weight portion, includes former below
Material：Fruice 122-348 part, potassium salt 2.3-3.7 part, microcosmic salt 1.4-3.4 part, nitrogen salt 1.2-2.3 part, calcium salt 0.7-1 part, magnesium salt
0.6-0.9 part, iodine salt 0.3-0.5 part, boron salt 0.2-0.4 part, molybdenum salt 0.2-0.3 part, zinc salt 0.3-0.4 part, manganese salt 0.1-0.3
Part, mantoquita 0.1-0.2 part, iron salt 0.1-0.2 part, vitamin 0.09-0.25 part, aminoacid 0.08-0.2 part, indolebutyric acid
0.05-0.1 part, gibberellins 0.06-0.09 part, 6- benzyl gland fat purine 0.04-0.08 part, naphthalene acetic acid 0.06-0.09 part, kinetins
0.03-0.06 part, conditioner 0.17-0.22 part, cosolvent 1.4-2.8 part, dispersant 1.2-2.3 part, compatilizer 1-1.9 part,
Stabilizer 0.9-1.8 part, antibacterial 1.5-2.8 part, water 2500-3500 part；
Described fruice is Fructus Citri Limoniae juice；
Described potassium salt is potassium nitrate；
Described microcosmic salt is calcium phosphate；
Described nitrogen salt is ammonium carbonate；
Described calcium salt is sulphuric acid Asia calcium；
Described magnesium salt is sulphuric acid Asia magnesium；
Described iodine salt is potassium iodate；
Described boron salt is potassium borate；
Described molybdenum salt is sodium molybdate；
Described zinc salt is zinc chloride；
Described manganese salt is manganous sulphate；
Described mantoquita is copper sulfate；
Described iron salt is iron chloride；
Described vitamin is Vitamin E；
Described aminoacid is proline；
The described Radix Asparagi tissue cultured seedling culture medium improving asparagine, its preparation method comprises the following steps：
S1：Fruit is blended after Fructus Citri Limoniae peeling with pulper, gained was squeezed the juice 3000-5000 mesh sieve, prepared fruice；
S2：By the prepared fruice of step S1, potassium salt, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodine salt, boron salt, molybdenum salt, zinc salt, manganese
Salt, mantoquita, iron salt, vitamin, aminoacid, indolebutyric acid, gibberellins, 6- benzyl gland fat purine, naphthalene acetic acid, kinetins, cosolvent,
Dispersant, compatilizer, antibacterial, water mixing, are 95-135W in microwave power, temperature is 64-70 DEG C, and rotating speed is 200-400r/
28-52min, prepared mixed liquor A is stirred under min；
S3：Add conditioner, stabilizer in the mixed liquor A being obtained to step S2, be 47-53 DEG C in temperature, rotating speed is 100-
14-24min, prepared mixed liquid B is stirred under 300r/min；
S4：The pH value of the mixed liquid B that step S3 is obtained is adjusted to 5.7-6.4, is then 134-136 DEG C in temperature, pressure is
Under 210-230KPa, sterilize 4-6min, and the Radix Asparagi tissue cultured seedling culture medium that improve asparagine is obtained.
2. improve the Radix Asparagi tissue cultured seedling culture medium of asparagine according to claim 1 it is characterised in that described conditioner is
Humic acid.
3. improve the Radix Asparagi tissue cultured seedling culture medium of asparagine according to claim 1 it is characterised in that described cosolvent is
Polyvinylpyrrolidone.
4. according to claim 1 improve asparagine Radix Asparagi tissue cultured seedling culture medium it is characterised in that described dispersant with
Weight portion is unit, including following raw material：Potassium carboxymethylcellulose 3-7 part, agar 2-4 part, modified starch 3-5 part, polypropylene
Sour sodium 2-3 part, sodium alginate 1-2 part.
5. improve the Radix Asparagi tissue cultured seedling culture medium of asparagine according to claim 1 it is characterised in that described compatilizer is
Dupont EEA2112AC.
6. improve the Radix Asparagi tissue cultured seedling culture medium of asparagine according to claim 1 it is characterised in that described stabilizer is
Carrageenan.
7. improve the Radix Asparagi tissue cultured seedling culture medium of asparagine according to claim 1 it is characterised in that described antibacterial is
Methyl-furfuryl fumarate.
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