CN106472309B - A kind of asparagus fern tissue-cultured seedling culture medium for improving asparagine - Google Patents
A kind of asparagus fern tissue-cultured seedling culture medium for improving asparagine Download PDFInfo
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- CN106472309B CN106472309B CN201610877881.4A CN201610877881A CN106472309B CN 106472309 B CN106472309 B CN 106472309B CN 201610877881 A CN201610877881 A CN 201610877881A CN 106472309 B CN106472309 B CN 106472309B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention discloses a kind of asparagus fern tissue-cultured seedling culture medium for improving asparagine, including following raw material：Fruit syrup, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salts, salt compounded of iodine, boron salt, molybdenum salt, zinc salt, manganese salt, mantoquita, molysite, vitamin, amino acid, indolebutyric acid, gibberellin, 6 benzyl gland fat purines, methyl α-naphthyl acetate, kinetin, conditioner, cosolvent, dispersant, compatilizer, stabilizer, antiseptic, water.The seedling quality that the asparagus fern tissue-cultured seedling culture medium tissue culture asparagus fern using the present invention for improving asparagine is obtained is good, and survival rate is high and active ingredient asparagine is high.
Description
【Technical field】
The invention belongs to asparagus fern planting technology field, and in particular to a kind of asparagus fern tissue-cultured seedling culture for improving asparagine
Base.
【Background technology】
Asparagus fern is a kind of common Chinese medicinal material, has the effect of nourishing Yin and promoting production of body fluid, moistening lung clears away heart-fire.In recent years, in the market pair
The demand of wild asparagus fern significantly increases, cause wild asparagus fern by destructive therefore asparagus fern artificial cultivation technique also progressively
Grow up.Since most asparagus fern is all that scattered plantation is carried out by peasant currently on the market, kind is more miscellaneous, and asparagus fern has
Effect component asparagine content is difficult to be protected.
【The content of the invention】
The present invention provides a kind of asparagus fern tissue-cultured seedling culture medium for improving asparagine, how to solve existing tissue culture medium (TCM)
The content of the principle active component asparagine of asparagus fern is improved, the problems such as increasing economic efficiency.Raising Tianmen using the present invention
The seedling quality that the asparagus fern tissue-cultured seedling culture medium tissue culture asparagus fern of winter element is obtained is good, survival rate height and active ingredient asparagine
It is high.
To solve above technical problem, the present invention uses following technical scheme：
A kind of asparagus fern tissue-cultured seedling culture medium for improving asparagine, in units of parts by weight, including following raw material：Fruit syrup
122-348 parts, 2.3-3.7 parts of sylvite, 1.4-3.4 parts of microcosmic salt, 1.2-2.3 parts of nitrogen salt, 0.7-1 parts of calcium salt, magnesium salts 0.6-0.9
Part, 0.3-0.5 parts of salt compounded of iodine, 0.2-0.4 parts of boron salt, 0.2-0.3 parts of molybdenum salt, 0.3-0.4 parts of zinc salt, 0.1-0.3 parts of manganese salt, mantoquita
0.1-0.2 parts, 0.1-0.2 parts of molysite, 0.09-0.25 parts of vitamin, 0.08-0.2 parts of amino acid, indolebutyric acid 0.05-0.1
Part, 0.06-0.09 parts of gibberellin, 0.04-0.08 parts of 6- benzyl glands fat purine, 0.06-0.09 parts of methyl α-naphthyl acetate, kinetin 0.03-
0.06 part, 0.17-0.22 parts of conditioner, 1.4-2.8 parts of cosolvent, 1.2-2.3 parts of dispersant, 1-1.9 parts of compatilizer, stabilizer
0.9-1.8 parts, 1.5-2.8 parts of antiseptic, 2500-3500 parts of water；
The fruit syrup is lemon juice；
The sylvite is potassium nitrate；
The microcosmic salt is calcium phosphate；
The nitrogen salt is ammonium carbonate；
The calcium salt is calcium sulfite；
The magnesium salts is magnesium sulfite；
The salt compounded of iodine is Potassiumiodate；
The boron salt is potassium borate；
The molybdenum salt is sodium molybdate；
The zinc salt is zinc chloride；
The manganese salt is manganese sulfate；
The mantoquita is copper sulphate；
The molysite is iron chloride；
The vitamin is vitamin E；
The amino acid is proline；
The asparagus fern tissue-cultured seedling culture medium for improving asparagine, its preparation method comprise the following steps：
S1：Fruit will be blended with pulper after lemon peeling, gained was squeezed the juice 3000-5000 mesh sieves, and fruit syrup is made；
S2：By fruit syrup made from step S1, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salts, salt compounded of iodine, boron salt, molybdenum salt, zinc salt,
Manganese salt, mantoquita, molysite, vitamin, amino acid, indolebutyric acid, gibberellin, 6- benzyl gland fats purine, methyl α-naphthyl acetate, kinetin, hydrotropy
Agent, dispersant, compatilizer, antiseptic, water mixing, are 95-135W in microwave power, temperature is 64-70 DEG C, rotating speed 200-
28-52min is stirred under 400r/min, mixed liquor A is made；
S3：Conditioner, stabilizer are added into mixed liquor A made from step S2, is 47-53 DEG C in temperature, rotating speed is
14-24min is stirred under 100-300r/min, mixed liquid B is made；
S4：The pH value of mixed liquid B made from step S3 is adjusted to 5.7-6.4, is then 134-136 DEG C in temperature, pressure
Power is under 210-230KPa, and sterilize 4-6min, and the asparagus fern tissue-cultured seedling culture medium for improving asparagine is made.
Further, the conditioner is humic acid.
Further, the cosolvent is polyvinylpyrrolidone.
Further, the dispersant is in units of parts by weight, including following raw material：3-7 parts of potassium carboxymethylcellulose, fine jade
2-4 parts of fat, 3-5 parts of modified starch, 2-3 parts of Sodium Polyacrylate, 1-2 parts of sodium alginate.
Further, the compatilizer is Dupont EEA2112AC.
Further, the stabilizer is carragheen.
Further, the antiseptic is methyl-furfuryl fumarate.
The effect of medium component of the present invention is as follows：
The accumulation being added with beneficial to polyoses content of fruit syrup.
Apple polyphenol can make cell growth slow, can also promote accumulation of flavonoids as a kind of antioxidant.
The nutritive effect of potassium element can improve photosynthetic intensity, promote the formation of starch and sugar in crop body, increase
Pretend the resistance and disease resistance of thing, moreover it is possible to improve crop and nitrogen is absorbed.
Phosphorus is to form the indispensable element such as Nuclear extract, lecithin.P elements can accelerate cell division, promote root
System and overground part accelerate growth, promote bud differentiation, ahead of time maturation, improve fruit quality.
Nitrogen is the essential element that protein is formed, and protein is the base substance in cellular plasm composition.Nitrogen energy
Promote the formation of protein and chlorophyll, make dark green leaf color, leaf area increase, promotes the assimilation of carbon, is conducive to yield increase, product
Matter improves.
It is slack-off to lack the growth of calcium plant meristem, calcium deficiency and disease are just in growing point and spire, when serious, leaf deformation
And chlorosis, there is necrotic spot at the edge of leaf.
Magnesium is the constituent of chlorophyll.If lacking magnesium, blade loses green, it is impossible to manufactures nutriment.Iodine is
The indispensable element of plant growth, is known as biological element, wisdom element.
Boron compares in plant to be concentrated in stem apex, the tip of a root, blade and floral organ, and the content in dicotyledon is usually
Higher than monocotyledon.Boron can improve the nitrogenase activity of legume rhizobium, increase amount of nitrogen fixation, and during boron deficiency, root, which stays, not to be sent out
Reach, influence amount of nitrogen fixation.Boron can also strengthen crop anti-adversity.
Molybdenum is nitrogen-fixing microorganism, is particularly fixed with the rhizobium of legume symbiosis necessary during air nitrogen.Together
When can promote leaf photosynthesis intensity again.Plant is short and small when lacking molybdenum, and chlorosis between blade arteries and veins, leaf margin is dried-up, inward curl, into withering
Listless state.
Zinc can promote crop to carry out photosynthesis, it is the constituent of a variety of enzymes, and crop growth occurs during zinc-deficiency
Stagnate.
Contain manganese in plant chloroplast, manganese can promote germination and seedling period to grow, and when manganese deficiency influences the light of crop
The accumulation of cooperation use, respiration, nitrate nitrogen in vivo.Chlorosis yellow between blade arteries and veins is shown as, there is brown spot.
The photosynthesis of copper participation plant, respiration, nitrogen metabolism, the resistance for strengthening plant.Plant strain growth is thin when lacking copper
Weak, young leaves jaundice is wilting withered, and blade tip curling is turned white, and has necrotic spot.
Iron is that chlorophyll formation is indispensable, is difficult transfer in plant body, so blade " chlorosis " is that plant lacks
The performance of iron, and this chlorosis is shown on young leaflet tablet first.
Vitamin has effect to taking root for plant, can promote plant establishment.
Amino acid is nutriment necessary to plant growth, special to plant growth due to the characteristic of amino acid in itself
It is that photosynthesis has unique facilitation, it can increase chlorophyll content of plant, improve the activity of enzyme, promote titanium dioxide
The infiltration of carbon, makes photosynthesis more vigorous, and to improving crop quality, increase Vc and sugared content suffer from important function.
Indolebutyric acid is mainly used for rooting of cuttings, can induce the formation of root substance, promotes cell differentiation and division, is conducive to
New root generates and the differentiation of fibrovascular system, promotes the formation of cutting adventitious root.
Gibberellin, is a kind of plant hormone being widely present.Its chemical constitution belongs to Diterpenes acid, is derived by four ring skeletons
And obtain, the growth of leaf and bud can be stimulated.
6-benzyladenine, Chinese nickname are 2- benayl aminopurines, N- benzyl adenosines, n-butyl acrylate, white or class
White crystal, is insoluble in water, is slightly soluble in ethanol, stablizes in acid, alkali, belongs to broad spectrum activity plant growth regulator, can promote plant
Cell growth, suppresses the degraded of plant chlorophyll, improves the content of amino acid, Delaying Leaf-Senescence etc..
Methyl α-naphthyl acetate (1-Naphthaleneacetic acid), abbreviation NAA, is a kind of organic compound, is a kind of readily soluble
In the colorless solid of organic solvent, its structure is substituted for No. 1 position of naphthalene with carboxymethyl, it is Plant Hormone growth
Element, is usually used in the root of hair powder or rooting agent of commercialization, is used when plant is using cuttage breeding.It can also be used for plant tissue
Culture.
Kinetin is a kind of non-natural basic element of cell division, and chemical name is 6- glycosyls adenine phosphate (or N6- furfuryls
Adenine).It is not soluble in water, it is dissolved in strong acid, alkali and glacial acetic acid；In addition to fissional effect is promoted, also have and delay
Excised leaf and cut flower withering, induced bud differentiation and development and the effect of increase stomatal aperture.
Conditioner can promote the development and beneficial microorganism activity of root system.
The solubility of cosolvent increase culture medium in a solvent.
Dispersant can prevent from flocculating, and prevent from settling.
Compatilizer refers to by means of intermolecular bonding force, promote incompatible two kinds of polymer to be incorporated in one, and then
Obtain the auxiliary agent of stable blend.
Stabilizer can increase the stability of solution, colloid, solid, mixture.
Antiseptic is can to make certain micro-organisms (bacterium, fungi, saccharomycete, algae and virus etc.) within a certain period of time
Growth or breeding be maintained at necessary chemical substance below horizontal.
The invention has the advantages that：
(1) seedling quality that the asparagus fern tissue-cultured seedling culture medium tissue culture asparagus fern using the present invention for improving asparagine is obtained
Good, survival rate is high and active ingredient asparagine is high；
(2) asparagus fern culture medium preparation method using the present invention, can shorten disinfecting time, improve sterilization effect reliability
With industrialization production efficiency, production cost is reduced；
(3) asparagus fern culture medium preparation method of the invention, it is easy to operate, it is easy to accomplish industrialization, standardization and standardization
Production.
【Embodiment】
For ease of more fully understanding the present invention, it is illustrated by following embodiments, these embodiments belong to the present invention's
Protection domain, but do not limit the scope of the invention.
In embodiment, the asparagus fern tissue-cultured seedling culture medium for improving asparagine, in units of parts by weight, including it is following
Raw material：122-348 parts of fruit syrup, 2.3-3.7 parts of sylvite, 1.4-3.4 parts of microcosmic salt, 1.2-2.3 parts of nitrogen salt, 0.7-1 parts of calcium salt, magnesium
0.6-0.9 parts of salt, 0.3-0.5 parts of salt compounded of iodine, 0.2-0.4 parts of boron salt, 0.2-0.3 parts of molybdenum salt, 0.3-0.4 parts of zinc salt, manganese salt 0.1-
0.3 part, 0.1-0.2 parts of mantoquita, 0.1-0.2 parts of molysite, 0.09-0.25 parts of vitamin, 0.08-0.2 parts of amino acid, indolebutyric acid
0.05-0.1 parts, 0.06-0.09 parts of gibberellin, 0.04-0.08 parts of 6- benzyl glands fat purine, 0.06-0.09 parts of methyl α-naphthyl acetate, kinetin
0.03-0.06 parts, 0.17-0.22 parts of conditioner, 1.4-2.8 parts of cosolvent, 1.2-2.3 parts of dispersant, 1-1.9 parts of compatilizer,
0.9-1.8 parts of stabilizer, 1.5-2.8 parts of antiseptic, 2500-3500 parts of water；
The fruit syrup is lemon juice；
The sylvite is potassium nitrate；
The microcosmic salt is calcium phosphate；
The nitrogen salt is ammonium carbonate；
The calcium salt is calcium sulfite；
The magnesium salts is magnesium sulfite；
The salt compounded of iodine is Potassiumiodate；
The boron salt is potassium borate；
The molybdenum salt is sodium molybdate；
The zinc salt is zinc chloride；
The manganese salt is manganese sulfate；
The mantoquita is copper sulphate；
The molysite is iron chloride；
The vitamin is vitamin E；
The amino acid is proline；
The conditioner is humic acid；
The cosolvent is polyvinylpyrrolidone；
The dispersant is in units of parts by weight, including following raw material：3-7 parts of potassium carboxymethylcellulose, 2-4 parts of agar,
3-5 parts of modified starch, 2-3 parts of Sodium Polyacrylate, 1-2 parts of sodium alginate；
The compatilizer is Dupont EEA2112AC；
The stabilizer is carragheen；
The antiseptic is methyl-furfuryl fumarate；
The asparagus fern tissue-cultured seedling culture medium for improving asparagine, its preparation method comprise the following steps：
S1：Fruit will be blended with pulper after lemon peeling, gained was squeezed the juice 3000-5000 mesh sieves, and fruit syrup is made；
S2：By fruit syrup made from step S1, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salts, salt compounded of iodine, boron salt, molybdenum salt, zinc salt,
Manganese salt, mantoquita, molysite, vitamin, amino acid, indolebutyric acid, gibberellin, 6- benzyl gland fats purine, methyl α-naphthyl acetate, kinetin, hydrotropy
Agent, dispersant, compatilizer, antiseptic, water mixing, are 95-135W in microwave power, temperature is 64-70 DEG C, rotating speed 200-
28-52min is stirred under 400r/min, mixed liquor A is made；
S3：Conditioner, stabilizer are added into mixed liquor A made from step S2, is 47-53 DEG C in temperature, rotating speed is
14-24min is stirred under 100-300r/min, mixed liquid B is made；
S4：The pH value of mixed liquid B made from step S3 is adjusted to 5.7-6.4, is then 134-136 DEG C in temperature, pressure
Power is under 210-230KPa, and sterilize 4-6min, and the asparagus fern tissue-cultured seedling culture medium for improving asparagine is made.
Embodiment 1
A kind of asparagus fern tissue-cultured seedling culture medium for improving asparagine, in units of parts by weight, including following raw material：Fruit syrup
250 parts, 3 parts of sylvite, 2.5 parts of microcosmic salt, 1.8 parts of nitrogen salt, 0.9 part of calcium salt, 0.8 part of magnesium salts, 0.4 part of salt compounded of iodine, 0.3 part of boron salt, molybdenum
0.2 part of salt, 0.3 part of zinc salt, 0.2 part of manganese salt, 0.1 part of mantoquita, 0.1 part of molysite, 0.13 part of vitamin, 0.14 part of amino acid, Yin
0.08 part of diindyl butyric acid, 0.08 part of gibberellin, 0.07 part of 6- benzyl gland fats purine, 0.08 part of methyl α-naphthyl acetate, 0.05 part of kinetin, conditioner
0.15 part, 2.2 parts of cosolvent, 1.8 parts of dispersant, 1.5 parts of compatilizer, 1.2 parts of stabilizer, 2.2 parts of antiseptic, 3000 parts of water；
The fruit syrup is lemon juice；
The sylvite is potassium nitrate；
The microcosmic salt is calcium phosphate；
The nitrogen salt is ammonium carbonate；
The calcium salt is calcium sulfite；
The magnesium salts is magnesium sulfite；
The salt compounded of iodine is Potassiumiodate；
The boron salt is potassium borate；
The molybdenum salt is sodium molybdate；
The zinc salt is zinc chloride；
The manganese salt is manganese sulfate；
The mantoquita is copper sulphate；
The molysite is iron chloride；
The vitamin is vitamin E；
The amino acid is proline；
The conditioner is humic acid；
The cosolvent is polyvinylpyrrolidone；
The dispersant is in units of parts by weight, including following raw material：6 parts of potassium carboxymethylcellulose, 3 parts of agar, be modified
4 parts of starch, 2 parts of Sodium Polyacrylate, 1 part of sodium alginate；
The compatilizer is Dupont EEA2112AC；
The stabilizer is carragheen；
The antiseptic is methyl-furfuryl fumarate；
The asparagus fern tissue-cultured seedling culture medium for improving asparagine, its preparation method comprise the following steps：
S1：Fruit will be blended with pulper after lemon peeling, gained was squeezed the juice 4000 mesh sieves, and fruit syrup is made；
S2：By fruit syrup made from step S1, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salts, salt compounded of iodine, boron salt, molybdenum salt, zinc salt,
Manganese salt, mantoquita, molysite, vitamin, amino acid, indolebutyric acid, gibberellin, 6- benzyl gland fats purine, methyl α-naphthyl acetate, kinetin, hydrotropy
Agent, dispersant, compatilizer, antiseptic, water mixing, are 120W in microwave power, temperature is 67 DEG C, and rotating speed is to be stirred under 300r/min
40min is mixed, mixed liquor A is made；
S3：Conditioner, stabilizer are added into mixed liquor A made from step S2, is 49 DEG C in temperature, rotating speed 200r/
18min is stirred under min, mixed liquid B is made；
S4：The pH value of mixed liquid B made from step S3 is adjusted to 6, is then 135 DEG C in temperature, pressure 220KPa
Under, sterilize 5min, and the asparagus fern tissue-cultured seedling culture medium for improving asparagine is made.
Embodiment 2
A kind of asparagus fern tissue-cultured seedling culture medium for improving asparagine, in units of parts by weight, including following raw material：Fruit syrup
122 parts, 2.3 parts of sylvite, 1.4 parts of microcosmic salt, 1.2 parts of nitrogen salt, 0.7 part of calcium salt, 0.6 part of magnesium salts, 0.3 part of salt compounded of iodine, 0.2 part of boron salt,
0.2 part of molybdenum salt, 0.3 part of zinc salt, 0.1 part of manganese salt, 0.1 part of mantoquita, 0.1 part of molysite, 0.09 part of vitamin, 0.08 part of amino acid,
0.05 part of indolebutyric acid, 0.06 part of gibberellin, 0.04 part of 6- benzyl gland fats purine, 0.06 part of methyl α-naphthyl acetate, 0.03 part of kinetin, conditioning
0.17 part of agent, 1.4 parts of cosolvent, 1.2 parts of dispersant, 1 part of compatilizer, 0.9 part of stabilizer, 1.5 parts of antiseptic, 2500 parts of water；
The fruit syrup is lemon juice；
The sylvite is potassium nitrate；
The microcosmic salt is calcium phosphate；
The nitrogen salt is ammonium carbonate；
The calcium salt is calcium sulfite；
The magnesium salts is magnesium sulfite；
The salt compounded of iodine is Potassiumiodate；
The boron salt is potassium borate；
The molybdenum salt is sodium molybdate；
The zinc salt is zinc chloride；
The manganese salt is manganese sulfate；
The mantoquita is copper sulphate；
The molysite is iron chloride；
The vitamin is vitamin E；
The amino acid is proline；
The conditioner is humic acid；
The cosolvent is polyvinylpyrrolidone；
The dispersant is in units of parts by weight, including following raw material：3 parts of potassium carboxymethylcellulose, 2 parts of agar, be modified
3 parts of starch, 2 parts of Sodium Polyacrylate, 1 part of sodium alginate；
The compatilizer is Dupont EEA2112AC；
The stabilizer is carragheen；
The antiseptic is methyl-furfuryl fumarate；
The asparagus fern tissue-cultured seedling culture medium for improving asparagine, its preparation method comprise the following steps：
S1：Fruit will be blended with pulper after lemon peeling, gained was squeezed the juice 3000 mesh sieves, and fruit syrup is made；
S2：By fruit syrup made from step S1, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salts, salt compounded of iodine, boron salt, molybdenum salt, zinc salt,
Manganese salt, mantoquita, molysite, vitamin, amino acid, indolebutyric acid, gibberellin, 6- benzyl gland fats purine, methyl α-naphthyl acetate, kinetin, hydrotropy
Agent, dispersant, compatilizer, antiseptic, water mixing, are 95W in microwave power, temperature is 64 DEG C, and rotating speed is to be stirred under 200r/min
52min is mixed, mixed liquor A is made；
S3：Conditioner, stabilizer are added into mixed liquor A made from step S2, is 47 DEG C in temperature, rotating speed 100r/
24min is stirred under min, mixed liquid B is made；
S4：The pH value of mixed liquid B made from step S3 is adjusted to 5.7, is then 134 DEG C in temperature, pressure is
Under 210KPa, sterilize 6min, and the asparagus fern tissue-cultured seedling culture medium for improving asparagine is made.
Embodiment 3
A kind of asparagus fern tissue-cultured seedling culture medium for improving asparagine, in units of parts by weight, including following raw material：Fruit syrup
348 parts, 3.7 parts of sylvite, 3.4 parts of microcosmic salt, 2.3 parts of nitrogen salt, 1 part of calcium salt, 0.9 part of magnesium salts, 0.5 part of salt compounded of iodine, 0.4 part of boron salt, molybdenum
0.3 part of salt, 0.4 part of zinc salt, 0.3 part of manganese salt, 0.2 part of mantoquita, 0.2 part of molysite, 0.25 part of vitamin, 0.2 part of amino acid, indoles
0.1 part of butyric acid, 0.09 part of gibberellin, 0.08 part of 6- benzyl gland fats purine, 0.09 part of methyl α-naphthyl acetate, 0.06 part of kinetin, conditioner
0.22 part, 2.8 parts of cosolvent, 2.3 parts of dispersant, 1.9 parts of compatilizer, 1.8 parts of stabilizer, 2.8 parts of antiseptic, 3500 parts of water；
The fruit syrup is lemon juice；
The sylvite is potassium nitrate；
The microcosmic salt is calcium phosphate；
The nitrogen salt is ammonium carbonate；
The calcium salt is calcium sulfite；
The magnesium salts is magnesium sulfite；
The salt compounded of iodine is Potassiumiodate；
The boron salt is potassium borate；
The molybdenum salt is sodium molybdate；
The zinc salt is zinc chloride；
The manganese salt is manganese sulfate；
The mantoquita is copper sulphate；
The molysite is iron chloride；
The vitamin is vitamin E；
The amino acid is proline；
The conditioner is humic acid；
The cosolvent is polyvinylpyrrolidone；
The dispersant is in units of parts by weight, including following raw material：7 parts of potassium carboxymethylcellulose, 4 parts of agar, be modified
5 parts of starch, 3 parts of Sodium Polyacrylate, 2 parts of sodium alginate；
The compatilizer is Dupont EEA2112AC；
The stabilizer is carragheen；
The antiseptic is methyl-furfuryl fumarate；
The asparagus fern tissue-cultured seedling culture medium for improving asparagine, its preparation method comprise the following steps：
S1：Fruit will be blended with pulper after lemon peeling, gained was squeezed the juice 5000 mesh sieves, and fruit syrup is made；
S2：By fruit syrup made from step S1, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salts, salt compounded of iodine, boron salt, molybdenum salt, zinc salt,
Manganese salt, mantoquita, molysite, vitamin, amino acid, indolebutyric acid, gibberellin, 6- benzyl gland fats purine, methyl α-naphthyl acetate, kinetin, hydrotropy
Agent, dispersant, compatilizer, antiseptic, water mixing, are 135W in microwave power, temperature is 70 DEG C, and rotating speed is to be stirred under 400r/min
28min is mixed, mixed liquor A is made；
S3：Conditioner, stabilizer are added into mixed liquor A made from step S2, is 53 DEG C in temperature, rotating speed 300r/
14min is stirred under min, mixed liquid B is made；
S4：The pH value of mixed liquid B made from step S3 is adjusted to 6.4, is then 136 DEG C in temperature, pressure is
Under 230KPa, sterilize 4min, and the asparagus fern tissue-cultured seedling culture medium for improving asparagine is made.
The asparagus fern tissue-cultured seedling culture medium using the present invention for improving asparagine improves the asparagus fern group of asparagine with tradition
Seedling culture medium (control group) tissue culture asparagus fern seedling is trained, condition of tissue culture is basically identical, 100 plants of each embodiment or control group tissue culture, knot
Fruit is as shown in the table.
As seen from table, the kind that the asparagus fern tissue-cultured seedling culture medium tissue culture asparagus fern using the present invention for improving asparagine is obtained
Seedling quality is good, and survival rate is high and active ingredient asparagine is high.
The specific implementation of the invention is not to be limited to these illustrations for above content, is led for technology belonging to the present invention
For the those of ordinary skill in domain, without departing from the inventive concept of the premise, some simple deduction or replace can also be made,
The scope of patent protection that the present invention is determined by the claims submitted should be all considered as belonging to.
Claims (6)
- A kind of 1. asparagus fern tissue-cultured seedling culture medium for improving asparagine, it is characterised in that in units of parts by weight, including following original Material：122-348 parts of fruit syrup, 2.3-3.7 parts of sylvite, 1.4-3.4 parts of microcosmic salt, 1.2-2.3 parts of nitrogen salt, 0.7-1 parts of calcium salt, magnesium salts 0.6-0.9 parts, 0.3-0.5 parts of salt compounded of iodine, 0.2-0.4 parts of boron salt, 0.2-0.3 parts of molybdenum salt, 0.3-0.4 parts of zinc salt, manganese salt 0.1-0.3 Part, 0.1-0.2 parts of mantoquita, 0.1-0.2 parts of molysite, 0.09-0.25 parts of vitamin, 0.08-0.2 parts of amino acid, indolebutyric acid 0.05-0.1 parts, 0.06-0.09 parts of gibberellin, 0.04-0.08 parts of 6- benzyl glands fat purine, 0.06-0.09 parts of methyl α-naphthyl acetate, kinetin 0.03-0.06 parts, 0.17-0.22 parts of conditioner, 1.4-2.8 parts of cosolvent, 1.2-2.3 parts of dispersant, 1-1.9 parts of compatilizer, 0.9-1.8 parts of stabilizer, 1.5-2.8 parts of antiseptic, 2500-3500 parts of water；The fruit syrup is lemon juice；The sylvite is potassium nitrate；The microcosmic salt is calcium phosphate；The nitrogen salt is ammonium carbonate；The calcium salt is calcium sulfite；The magnesium salts is magnesium sulfite；The salt compounded of iodine is Potassiumiodate；The boron salt is potassium borate；The molybdenum salt is sodium molybdate；The zinc salt is zinc chloride；The manganese salt is manganese sulfate；The mantoquita is copper sulphate；The molysite is iron chloride；The vitamin is vitamin E；The amino acid is proline；The dispersant is in units of parts by weight, including following raw material：3-7 parts of potassium carboxymethylcellulose, 2-4 parts of agar, be modified 3-5 parts of starch, 2-3 parts of Sodium Polyacrylate, 1-2 parts of sodium alginate；The asparagus fern tissue-cultured seedling culture medium for improving asparagine, its preparation method comprise the following steps：S1：Fruit will be blended with pulper after lemon peeling, gained was squeezed the juice 3000-5000 mesh sieves, and fruit syrup is made；S2：By fruit syrup, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salts, salt compounded of iodine, boron salt, molybdenum salt, zinc salt, manganese made from step S1 Salt, mantoquita, molysite, vitamin, amino acid, indolebutyric acid, gibberellin, 6- benzyl gland fats purine, methyl α-naphthyl acetate, kinetin, cosolvent, Dispersant, compatilizer, antiseptic, water mixing, are 95-135W in microwave power, temperature is 64-70 DEG C, rotating speed 200-400r/ 28-52min is stirred under min, mixed liquor A is made；S3：Conditioner, stabilizer are added into mixed liquor A made from step S2, is 47-53 DEG C in temperature, rotating speed 100- 14-24min is stirred under 300r/min, mixed liquid B is made；S4：The pH value of mixed liquid B made from step S3 is adjusted to 5.7-6.4, is then 134-136 DEG C in temperature, pressure is Under 210-230KPa, sterilize 4-6min, and the asparagus fern tissue-cultured seedling culture medium for improving asparagine is made.
- 2. the asparagus fern tissue-cultured seedling culture medium of asparagine is improved according to claim 1, it is characterised in that the conditioner is Humic acid.
- 3. the asparagus fern tissue-cultured seedling culture medium of asparagine is improved according to claim 1, it is characterised in that the cosolvent is Polyvinylpyrrolidone.
- 4. the asparagus fern tissue-cultured seedling culture medium of asparagine is improved according to claim 1, it is characterised in that the compatilizer is Dupont EEA2112AC.
- 5. the asparagus fern tissue-cultured seedling culture medium of asparagine is improved according to claim 1, it is characterised in that the stabilizer is Carragheen.
- 6. the asparagus fern tissue-cultured seedling culture medium of asparagine is improved according to claim 1, it is characterised in that the antiseptic is Methyl-furfuryl fumarate.
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