CN104255450A - Rapid propagation method of temstroemia gymnanthe - Google Patents
Rapid propagation method of temstroemia gymnanthe Download PDFInfo
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- CN104255450A CN104255450A CN201410445989.7A CN201410445989A CN104255450A CN 104255450 A CN104255450 A CN 104255450A CN 201410445989 A CN201410445989 A CN 201410445989A CN 104255450 A CN104255450 A CN 104255450A
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- ternstroemia gymnanthera
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Abstract
The invention relates to a rapid propagation method of temstroemia gymnanthe. The rapid propagation method comprises disinfection of explants, bud induction culture, multiple shoot proliferation, rooting culture and acclimatization and transplanting. A tissue culture method of temstroemia gymnanthe, disclosed by the invention, is simple and convenient to operate, is short in growth cycle, high in survival rate and stable in genetic character, and can obtain a great number of temstroemia gymnanthe seedlings in a short period.
Description
Technical field
The present invention relates to the quick-breeding method of Ternstroemia gymnanthera tissue cultures, belong to plant technology field.
Background technology
Ternstroemia gymnanthera,
ternstroemia gymnanthera(Wight et Arn.) Beddome., Theaceae, shrub or dungarunga, have another name called nakedanther ternstroemia leaf flower and fruit, and weighing lever is red, and Mo Hong cuts, and camellia is set, pig blood bavin, qi and blood rattan.Be distributed in the provinces such as Hubei, Hunan, Guizhou, Yunnan, Guangxi, Fujian, Guangdong, Taiwan.Also there are distribution in Japan, Cambodia, India.Be born in the evergreen broad-leaved forest of acid yellow soil, yellowish soil or border, between vertical distribution height above sea level 700 ~ 3500m more.Ternstroemia gymnanthera strong adaptability, again resistance to the moon, tree crown is perfectly round, and branches and leaves stereovision is strong, and leaf plumpness enters the winter and turns bright red, is more excellent lower wood, suitable planting at the place such as sylvan life, border, based on planting stock.Like dark and damp environment, grow vigorous under broad-leaved evergreen.Also light is liked, more cold-resistant ,-10 DEG C of low temperature can be stood.Happiness acid soil, also can adapt to neutral soil and alkalescence soil.Well developed root system, wind resistance power is strong, and rudiment power is weak, poor growth, not resistance to intensity is pruned, but still can carry out light pruning, the growth of antipollution power strong Ternstroemia gymnanthera is comparatively slow, and raw hide perfume (or spice) is half heliophilous species, and anti-harmful gas is strong, be again the green tree species in factories and miness district, be also a kind of traditional Chinese medicine, flower can be used as medicine, can be clearing heat and detoxicating, eliminating stasis to subdue swelling, cures mainly mange itch, the diseases such as flu.Propagation method main is at present sowing and cottage propagation, and germination rate is lower, only has 50% ~ 60%.Utilize tissue culture method effectively can improve the reproductive efficiency of Ternstroemia gymnanthera, but there is no research both at home and abroad at present.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for quickly breeding of Ternstroemia gymnanthera, and adopt the Ternstroemia gymnanthera tissue culture method of the inventive method breeding easy, growth cycle is short, and survival rate is high, stabilization characteristics of genetics, can obtain a large amount of Ternstroemia gymnanthera seedling in a short time.
Technical problem to be solved by this invention is realized by following scheme:
Get Ternstroemia gymnanthera stem with bud, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 25min, 75% ethanol postincubation 30s on superclean bench, clorox process 12min, aseptic water washing 5-7 time, the Ternstroemia gymnanthera stem with bud access culture medium prescription that sterilization treatment is crossed is B
5carry out bud inducement cultivation in+IAA0.2mg/L+IBA0.5mg/l inducing culture, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx, the bud derived puts into proliferated culture medium B
5+ 2, 4-D0.2mg/L+TDZ0.3mg/L+ABA1mg/L, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx, photophase 10h, dark phase 14h, Multiplying culture Multiple Buds out puts into root media White+NAA0.2mg/L, culture of rootage condition is sucrose 20g/L, agar 6.5g/L, PH5, illumination 1000lx, humidity 60-70%, then test-tube plantlet is carried out acclimatization and transplants, careful taking-up is about the test-tube plantlet of 5cm, clean root medium, plant in perlite: cultivate in the matrix of peat soil=1:1, place greenhouse, use liquor potassic permanganate periodically sprinkle, after cultivating 20d, be transplanted into land for growing field crops, 30d adds up survival rate.
The Ternstroemia gymnanthera survival rate adopting the present invention to prepare is 95%, and the cycle is short, and output is large, and less energy consumption, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment 1
Get Ternstroemia gymnanthera stem with bud, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 25min, 75% ethanol postincubation 30s on superclean bench, clorox process 12min, aseptic water washing 5-7 time, the Ternstroemia gymnanthera stem with bud access culture medium prescription that sterilization treatment is crossed is carry out bud inducement cultivation in MS+IAA0.2mg/L+IBA0.5mg/l inducing culture, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx, the bud derived puts into proliferated culture medium B
5+ 2,4-D0.1mg/L+TDZ0.2mg/L+ABA1mg/L, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx, photophase 10h, dark phase 14h, Multiplying culture Multiple Buds out puts into root media White+NAA0.1mg/L, culture of rootage condition is sucrose 20g/L, agar 6.5g/L, PH5, illumination 1000lx, humidity 60-70%, then carries out acclimatization and transplants by test-tube plantlet, and Ternstroemia gymnanthera survival rate is 90%.
Embodiment 2
Get Ternstroemia gymnanthera stem with bud, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 25min, 75% ethanol postincubation 30s on superclean bench, clorox process 12min, aseptic water washing 5-7 time, the Ternstroemia gymnanthera stem with bud access culture medium prescription that sterilization treatment is crossed is carry out bud inducement cultivation in White+IAA0.2mg/L+IBA0.5mg/l inducing culture, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx, the bud derived puts into proliferated culture medium B
5+ 2,4-D0.2mg/L+TDZ0.5mg/L+ABA1mg/L, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx, photophase 10h, dark phase 14h, Multiplying culture Multiple Buds out puts into root media White+NAA0.2mg/L, culture of rootage condition is sucrose 20g/L, agar 6.5g/L, PH5, illumination 1000lx, humidity 60-70%, then carries out acclimatization and transplants by test-tube plantlet, and Ternstroemia gymnanthera survival rate is 91%.
Embodiment 3
Get Ternstroemia gymnanthera stem with bud, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 25min, 75% ethanol postincubation 30s on superclean bench, clorox process 12min, aseptic water washing 5-7 time, the Ternstroemia gymnanthera stem with bud access culture medium prescription that sterilization treatment is crossed is carry out bud inducement cultivation in White+IAA0.2mg/L+IBA0.5mg/l inducing culture, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx, the bud derived puts into proliferated culture medium B
5+ 2,4-D0.2mg/L+TDZ0.2mg/L+ABA1mg/L, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx, photophase 10h, dark phase 14h, Multiplying culture Multiple Buds out puts into root media White+NAA0.1mg/L, culture of rootage condition is sucrose 20g/L, agar 6.5g/L, PH5, illumination 1000lx, humidity 60-70%, then carries out acclimatization and transplants by test-tube plantlet, and Ternstroemia gymnanthera survival rate is 92%.
Embodiment 4
Get Ternstroemia gymnanthera stem with bud, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 25min, 75% ethanol postincubation 30s on superclean bench, clorox process 12min, aseptic water washing 5-7 time, the Ternstroemia gymnanthera stem with bud access culture medium prescription that sterilization treatment is crossed is carry out bud inducement cultivation in MS+IAA0.2mg/L+IBA0.5mg/l inducing culture, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx, the bud derived puts into proliferated culture medium B
5+ 2,4-D0.1mg/L+TDZ0.5mg/L+ABA1mg/L, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx, photophase 10h, dark phase 14h, Multiplying culture Multiple Buds out puts into root media White+NAA0.1mg/L, culture of rootage condition is sucrose 20g/L, agar 6.5g/L, PH5, illumination 1000lx, humidity 60-70%, then carries out acclimatization and transplants by test-tube plantlet, and Ternstroemia gymnanthera survival rate is 93%.
Claims (6)
1. a method for quickly breeding for Ternstroemia gymnanthera, comprise the acquisition of aseptic explant, the induction of bud, the propagation of Multiple Buds, root induction, field acclimatization and transplants, its key step is as follows:
(1) get Ternstroemia gymnanthera stem with bud, sterilization method conveniently carries out sterilization treatment to it;
(2) Ternstroemia gymnanthera stem with bud access culture medium prescription step (1) sterilization treatment crossed is MS/White/B
5bud inducement cultivation is carried out, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx in+IAA0.2mg/L+IBA0.5mg/l inducing culture;
(3) get the bud that step (2) derives and put into proliferated culture medium B
5+ 2,4-D0.1-0.2mg/L+TDZ0.2-0.5mg/L+ABA1mg/L, additional saccharose 30g/L, agar 8g/L, PH5, illumination 1000lx, photophase 10h, dark phase 14h;
(4) get step (3) Multiplying culture Multiple Buds out and put into root media White+NAA0.1-0.2mg/L;
(5) get step (4) take root after Ternstroemia gymnanthera test-tube plantlet first carry out indoor hardening cultivation, be then transplanted to land for growing field crops and cultivate.
2. according to the method for quickly breeding of a kind of Ternstroemia gymnanthera according to claim 1, it is characterized in that: the acquisition of the aseptic bud of Ternstroemia gymnanthera described in step (1) is, get Ternstroemia gymnanthera stem with bud, first in bleaching powder, soak 3min, surface blot removed by hairbrush, running water 25min, 75% ethanol postincubation 30s on superclean bench, clorox process 12min, aseptic water washing 5-7 time.
3. according to the method for quickly breeding of a kind of Ternstroemia gymnanthera according to claim 1, it is characterized in that: step (2) inducing culture have employed three kinds of medium MS, White, B
5screen its inducing effect.
4. according to the method for quickly breeding of a kind of Ternstroemia gymnanthera according to claim 1, it is characterized in that: addition of ABA1mg/L in step (3) proliferated culture medium, the propagation of Multiple Buds can be promoted.
5. according to the method for quickly breeding of a kind of Ternstroemia gymnanthera according to claim 1, it is characterized in that: the culture of rootage condition in step (4) is sucrose 20g/L, agar 6.5g/L, PH5, illumination 1000lx, humidity 60-70%.
6. according to the method for quickly breeding of a kind of Ternstroemia gymnanthera according to claim 1, it is characterized in that: in step (5), the hardening cultural method of Ternstroemia gymnanthera is the test-tube plantlet that careful taking-up is about 5cm, clean root medium, plant in perlite: cultivate in the matrix of peat soil=1:1, place greenhouse, use liquor potassic permanganate periodically sprinkle, after cultivating 20d, be transplanted into land for growing field crops, 30d adds up survival rate.
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| CN201410445989.7A CN104255450A (en) | 2014-09-04 | 2014-09-04 | Rapid propagation method of temstroemia gymnanthe |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111684977A (en) * | 2020-06-15 | 2020-09-22 | 上饶市林业科学研究所 | Method for cultivating landscape seedlings of thick-skinned fragrant garden forest |
| CN116158351A (en) * | 2023-03-27 | 2023-05-26 | 广东省林业科学研究院 | Tissue culture seedling method of excellent family of nux vomica |
-
2014
- 2014-09-04 CN CN201410445989.7A patent/CN104255450A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111684977A (en) * | 2020-06-15 | 2020-09-22 | 上饶市林业科学研究所 | Method for cultivating landscape seedlings of thick-skinned fragrant garden forest |
| CN116158351A (en) * | 2023-03-27 | 2023-05-26 | 广东省林业科学研究院 | Tissue culture seedling method of excellent family of nux vomica |
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Application publication date: 20150107 |