CN103371093A - Azalea tissue culture seedling out-bottle rooting method - Google Patents

Azalea tissue culture seedling out-bottle rooting method Download PDF

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CN103371093A
CN103371093A CN2013103243301A CN201310324330A CN103371093A CN 103371093 A CN103371093 A CN 103371093A CN 2013103243301 A CN2013103243301 A CN 2013103243301A CN 201310324330 A CN201310324330 A CN 201310324330A CN 103371093 A CN103371093 A CN 103371093A
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bottle
seedling
tissue culture
azalea
group training
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CN103371093B (en
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朱春艳
顾宏辉
盛小光
朱丹华
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HANGZHOU BOTANICAL GARDEN
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Abstract

The invention relates to an azalea tissue culture seedling out-bottle rooting method which includes the steps of 1) selecting out-bottle rooting time and site; 2) hardening bottle seedlings; 3) preparing substrate; 4) cutting tissue culture seedlings; 5) seedling hardening after cutting and light and water management. An effective azalea tissue culture seedling out-bottle rooting generic technology is provided, the bottleneck that some azalea subgenus tissue culture seedlings are difficult in rooting is solved, and the method is applicable to most azalea subgenus and varieties. By the method, an azalea tissue culture rapid propagation system is completed, tissue culture in-bottle rooting is unnecessary, tissue culture space is saved by more than 50%, cost of tissue culture seedlings is saved by more than 30%, tissue cycle is shortened greatly, tissue culture seedling out-bottle rooting rate can be reach to 80-95%, and great significance is provided for improving tissue culture industrialization level.

Description

A kind of method of azalea group training outside sprout-cultivating-bottle radication
Technical field
The present invention relates to field of plant tissue culture technique, particularly relate to a kind of method of azalea group training outside sprout-cultivating-bottle radication.
Background technology
Azalea ( Rhododendron) be world-renowned ornamental flower, nearly thousand kinds approximately of whole world azalea initial specieses, China accounts for about 60%, is azalea cradle and distribution center.The whole world surpasses 10,000 by the kind that hybridization obtains, and germ plasm resource is very abundant.Of a great variety, the large flower and brilliant color of azalea has high ornamental value, is described as to spend middle Xisi, famous beauty in the late Spring and Autumn Period.China's azalea resource various places that spread all over the country, wherein Yunnan, Guizhou, Sichuan, the Tibet resource with the southwest is the abundantest especially.In gardens utilized, it was the azalea industrial base of representative that China has formed Fujian, Zhejiang, Liaoning, and its industry is very huge, has critical role in the industry of flowers and plants.
In the azalea industry, the breeding of new germ plasm new varieties is important steps.Except traditional seed and cuttage breeding method, the extensive use in the azalea breeding of modern biotechnology group culturation rapid propagating technology has promoted the fast development of cuckoo industrialization.Cuckoo group culturation rapid propagating technology system is made of numerous sport technique segments, and wherein group training seedling rooting technology is one of important composition.In recent years, Chinese scholars has proposed group training outside sprout-cultivating-bottle radication technology, and this technology group can be trained taking root of seedling and domestication combines, and energy simplification group training program, the shortening group training seedling production cycle, improves the effects such as reproduction coefficient.
Domestic research to the outside sprout-cultivating-bottle technology is more.Wu Lijun etc. have reported photinia glabra group training outside sprout-cultivating-bottle radication technology (Fujian Forestry science and technology, 2007,34(4): 4-7), Cheng Shuyun etc. have reported (the agricultural science and technology communication of blueberry tissue culture outside sprout-cultivating-bottle radication technology, 2009(4): 49-50), Huang Zhuozhong has summarized Advance in non-tube rootage technology (Guangxi Agricultural science, 2007,38(2): 124-126), studies show that more than the application of outside sprout-cultivating-bottle technology is very extensive, but document shows, (Zhu Chunyan etc. in our research, mentioning, China's agronomy circular, 2006,22:335-337; Gardens science and technology, 2012,2:13-14), the domestic report that azalea outside sprout-cultivating-bottle technology is not all arranged.Deng Guowen etc. have declared the method patent of invention (201010589834.2) of culture of rootage in a kind of alpine rose group training seedling greenhouse and acclimatization and transplants, its main method comprises that a bottle seedling is emerged, water planting is taken root, the steps such as transplantation of seedlings and the rear management of transplanting of taking root, core content is the water planting technology of taking root, and this and the used technology of this invention have larger difference.
Patent of the present invention is through 10 years of researches, sum up the azalea outside sprout-cultivating-bottle technology that succeeds more than 30 kind of cuckoo of a plurality of azalea subgenus, this technology can reduce production costs more than 30%, saving group training space is more than 50%, the shortening group training cycle is more than 1 month, and azalea tissue-culturing rapid propagation system perfect had the important technology meaning.
Summary of the invention
In order to solve above-mentioned technical problem, the purpose of this invention is to provide the method for a kind of azalea group training outside sprout-cultivating-bottle radication, breeding for the batch production of the foundation of azalea tissue-culturing rapid propagation system, group training seedling provides technical support.
In order to realize above-mentioned purpose, the present invention has adopted following technical scheme:
A kind of method of azalea group training outside sprout-cultivating-bottle radication, the method comprises the steps:
1) outside sprout-cultivating-bottle time and place are selected: do not having under the artificial temperature controlled condition, mean temperature of air is not higher than 20 ℃, carries out the outside sprout-cultivating-bottle operation March in October to the next year that selection is annual; Taking root needs booth or greenhouse by solar heat condition, and needs little shed to place the cave dish;
2) bottle seedling hardening: after the cultivation of strong seedling culture base, the long bottle seedling to 3-4cm of selection group training seedling plant height; The bottle seedling is moved on to the group training from group training chamber outdoor, placed first under the natural daylight condition 2-3 days; After bottle cap is opened twice every day, in afternoon in the morning each once, each opening time is 1 hour, continues 3-5 days;
3) matrix is prepared: selecting the pH value is 5-6, contains peat, perlite, vermiculite, sterile mixing commodity matrix, or the autogamy volume ratio is peat: the mixed-matrix of perlite: vermiculite=2:1:1; Every cube of the matrix of autogamy is with the sterilization that carries out disinfection of 20 grams, 50% carbendazol wettable powder; Matrix is sub-packed in the standard cave dish in 105 holes or 128 holes, waters permeable for subsequent use;
4) group training seedling cuttage: in booth, the group training seedling of finishing hardening is taken out from bottle, clean the medium that is bonded at root, pluck the blade than the bottom; Select of uniform size group of training seedling, with the bamboo let punching seed is planted in the dish of cave; Behind the full dish of kind the seedling water is coiled in the cave and irrigate, be positioned over subsequently in the little shed of sunshade net of film and 50%-70% shading rate; Behind whole cuttage end-of-jobs, use the broad-spectrum germicide spray disinfection;
5) hardening and light water management after the cuttage: after cuttage, keep sealing and sunshade in 5-7 days; Then remove the sunshade net on the little shed, film is taken off at the film two ends, and afternoon every morning, each 1 time ventilation was 1 hour, 5-7 days altogether; In the phase of taking root, evening and whole winter generally need not be opened little shed, only have daytime and carry out two ends during high temperature to ventilate; Group is trained packing of seedling after cuttage 1-2 month; During this time, continue to use broad-spectrum germicide spray disinfection 1-2 time; Moisture management is accomplished not do and is watered, and in the phase of taking root, watering is no more than 2 times.
The invention has the beneficial effects as follows:
1, set up effective cuckoo group flower training outside sprout-cultivating-bottle radication common technology, solved the technical bottleneck of part azalea subgenus group training seedling rooting difficulty, can be applied in the various and kind of most of azalea subgenus.
2, azalea tissue-culturing rapid propagation system that the present invention is perfect, need be through the program of taking root in the tissue culture bottle, saving group training space, chamber reaches more than 50%, saving group training seedling cost is more than 30%, greatly shortened the group training cycle, and realization group training outside sprout-cultivating-bottle radication rate reaches 80%-95%, promotes significant to group training industrialization level.
Embodiment
The present invention is described in further detail by following examples, but content of the present invention is not limited to this.
Embodiment 1:Evergreen broad-leaved cuckoo initial species and kind group training outside sprout-cultivating-bottle radication method are such as initial species Rhododendron fortuneilindl., crossbreed Lord Robert etc.
The method is carried out as follows:
1) outside sprout-cultivating-bottle time and place are selected: do not having under the artificial temperature controlled condition, mean temperature of air is not higher than 20 ℃, carries out the outside sprout-cultivating-bottle operation October to November as selecting; Utilize the booth of southern area, consolidation is smooth, and is equipped with little shed placement cave dish;
2) bottle seedling hardening: after the cultivation of strong seedling culture base, the long bottle seedling to 3-4cm of selection group training seedling plant height.The bottle seedling is moved on to the group training from group training chamber outdoor, placed first under the natural daylight condition 2 days; After bottle cap is opened twice every day, in afternoon in the morning each once, each opening time is 1 hour, continues 3 days;
3) matrix is prepared: selecting the pH value is 5-6, contains peat, perlite, vermiculite, and sterile mixing is acid commodity matrix; Matrix is sub-packed in the standard cave dish in 128 holes, waters permeable for subsequent use;
4) group training seedling cuttage: in booth, will from bottle, take out through the group training seedling of hardening, clean the medium that is bonded at root, pluck the blade than the bottom.Select of uniform size group of training seedling, with the bamboo let punching seed is planted in the dish of cave; Behind the full dish of kind the seedling water is coiled in the cave and irrigate, be positioned over subsequently in the little shed of sunshade net of film and 70% shading rate; Behind whole cuttage end-of-jobs, use broad-spectrum germicide carbendazim spray disinfection;
5) hardening and light water management after the cuttage: after cuttage, keep sealing and sunshade in 5 days; Then remove the sunshade net on the little shed, film is taken off at the film two ends, and in afternoon in the morning ventilated 1 hour twice of every day (each 1 time), and totally 5; In the phase of taking root, evening and whole winter generally need not be opened little shed, only have daytime and carry out two ends during high temperature to ventilate; Organize packing of training seedling after 1 month in cuttage; During this time, continue to use broad-spectrum germicide carbendazim spray disinfection 1-2 time; Moisture management is accomplished not do and is watered, and generally in the phase of taking root, waters 2 times.
Embodiment 2:Azalea initial species and kind group training outside sprout-cultivating-bottle radication method are such as the blue English of kind, large cinnabar etc.
The method is carried out as follows:
1) outside sprout-cultivating-bottle time and place are selected: do not having under the artificial temperature controlled condition, mean temperature of air is not higher than 20 ℃, carries out the outside sprout-cultivating-bottle operation December to January as selecting; Utilize the booth of southern area, consolidation is smooth, and is equipped with little shed placement cave dish;
2) bottle seedling hardening: after the cultivation of strong seedling culture base, the long bottle seedling to 3-4cm of selection group training seedling plant height; The bottle seedling is moved on to the group training from group training chamber outdoor, placed first under the natural daylight condition 3 days; After bottle cap is opened twice every day, in afternoon in the morning each once, each opening time is 1 hour, continues 5 days;
3) matrix is prepared: the autogamy volume ratio is peat: the mixed-matrix of perlite: vermiculite=2:1:1, and every cube of matrix is with the sterilization that carries out disinfection of 20 grams, 50% carbendazol wettable powder; Matrix is sub-packed in the standard cave dish in 128 holes, waters permeable for subsequent use;
4) group training seedling cuttage: in booth, the group training seedling of finishing hardening is taken out from bottle, clean the medium that is bonded at root, pluck the blade than the bottom; Select of uniform size group of training seedling, with the bamboo let punching seed is planted in the dish of cave; Behind the full dish of kind the seedling water is coiled in the cave and irrigate, be positioned over subsequently in the little shed of sunshade net of film and 50% shading rate; Behind whole cuttage end-of-jobs, use broad-spectrum germicide tpn spray disinfection;
5) hardening and light water management after the cuttage: after cuttage, keep sealing and sunshade in 7 days; Then remove the sunshade net on the little shed, film is taken off at the film two ends, and ventilated totally 7 days every day twice (each 1 time) 1 hour in afternoon in the morning; In the phase of taking root, evening and whole winter are not generally opened little shed, only have daytime and carry out two ends during high temperature to ventilate; Organize packing of training seedling after 2 months in cuttage; During this time, continue to use broad-spectrum germicide tpn spray disinfection 1-2 time; Moisture management is accomplished not do and is watered, and generally in the phase of taking root, waters 1 time.
Embodiment 3:Fallen leaves cuckoo initial species and kind group training outside sprout-cultivating-bottle radication method are such as initial species Chinese azalea, the red maple cuckoo of kind etc.;
Transplanting time is chosen in the 2-3 month, and the cave dish is selected 105 holes, and little shed shading rate selects 70%, the spraying of broad-spectrum germicide selection topsin, and very fast because transplanting the rising of rear 4-5 month temperature, little shed wants timely two ends to take off the film ventilation when higher to daytime temperature; Use during this time broad-spectrum germicide topsin spray disinfection 2 times, water during taking root 2 times; All the other measures and method are with embodiment 1.

Claims (1)

1. the method for an azalea group training outside sprout-cultivating-bottle radication is characterized in that the method comprises the steps:
1) outside sprout-cultivating-bottle time and place are selected: do not having under the artificial temperature controlled condition, mean temperature of air is not higher than 20 ℃, carries out the outside sprout-cultivating-bottle operation March in October to the next year that selection is annual; Taking root needs booth or greenhouse by solar heat condition, and needs little shed to place the cave dish;
2) bottle seedling hardening: after the cultivation of strong seedling culture base, the long bottle seedling to 3-4cm of selection group training seedling plant height; The bottle seedling is moved on to the group training from group training chamber outdoor, placed first under the natural daylight condition 2-3 days; After bottle cap is opened twice every day, in afternoon in the morning each once, each opening time is 1 hour, continues 3-5 days;
3) matrix is prepared: selecting the pH value is 5-6, contains peat, perlite, vermiculite, sterile mixing commodity matrix, or the autogamy volume ratio is peat: the mixed-matrix of perlite: vermiculite=2:1:1; Every cube of the matrix of autogamy is with the sterilization that carries out disinfection of 20 grams, 50% carbendazol wettable powder; Matrix is sub-packed in the standard cave dish in 105 holes or 128 holes, waters permeable for subsequent use;
4) group training seedling cuttage: in booth, the group training seedling of finishing hardening is taken out from bottle, clean the medium that is bonded at root, pluck the blade than the bottom; Select of uniform size group of training seedling, with the bamboo let punching seed is planted in the dish of cave; Behind the full dish of kind the seedling water is coiled in the cave and irrigate, be positioned over subsequently in the little shed of sunshade net of film and 50%-70% shading rate; Behind whole cuttage end-of-jobs, use the broad-spectrum germicide spray disinfection;
5) hardening and light water management after the cuttage: after cuttage, keep sealing and sunshade in 5-7 days; Then remove the sunshade net on the little shed, film is taken off at the film two ends, and afternoon every morning, each 1 time ventilation was 1 hour, 5-7 days altogether; In the phase of taking root, evening and whole winter generally need not be opened little shed, only have daytime and carry out two ends during high temperature to ventilate; Group is trained packing of seedling after cuttage 1-2 month; During this time, continue to use broad-spectrum germicide spray disinfection 1-2 time; Moisture management is accomplished not do and is watered, and in the phase of taking root, watering is no more than 2 times.
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CN103548653A (en) * 2013-11-01 2014-02-05 重庆文理学院 Honeysuckle tissue culture seedling non-tube rootage method
CN103880564A (en) * 2014-03-11 2014-06-25 何国森 Preparation method of rhododendron culture medium
CN104541838A (en) * 2014-09-30 2015-04-29 余大春 Azalea cuttage breeding technique
CN104542284A (en) * 2014-12-30 2015-04-29 北京林业大学 Tissue culture rapid propagation method for rhododendron irroratum
CN104541839A (en) * 2014-09-30 2015-04-29 余大春 Azalea wild-imitated cultivation technique
CN105794476A (en) * 2016-04-08 2016-07-27 石家庄市神州花卉研究所有限公司 One-film double-greenhouse seed seedling culture method for wild alpine roses
CN106258959A (en) * 2016-08-08 2017-01-04 广州华苑园林股份有限公司 A kind of Folium Rhododendri Simsii quick breeding method for tissue culture
CN107182784A (en) * 2017-06-12 2017-09-22 云南省热带作物科学研究所 A kind of efficient acclimatization and transplantses method of rubber tree tissue-cultured seedling and rubber tree seedling culture method
CN107278686A (en) * 2017-06-06 2017-10-24 山东大丰园农业有限公司 A kind of American red-maple tissue culture outside sprout-cultivating-bottle radication agent and method for culturing seedlings
CN107494264A (en) * 2017-08-31 2017-12-22 地缘(厦门)生物科技有限公司 A kind of method of Pterospermi Heterophylli tissue culture outside sprout-cultivating-bottle radication
CN108848977A (en) * 2017-12-20 2018-11-23 江苏省林业科学研究院 A kind of promotion blueberry tissue-cultured seedling rooting method
CN109997537A (en) * 2019-05-21 2019-07-12 黑龙江伊蓝生物科技有限公司 The micro- plug bottle ex vitro rooting technique of indigo fruit tissue culture subculture unrooted test tube seedling

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Cited By (14)

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Publication number Priority date Publication date Assignee Title
CN103548653A (en) * 2013-11-01 2014-02-05 重庆文理学院 Honeysuckle tissue culture seedling non-tube rootage method
CN103880564A (en) * 2014-03-11 2014-06-25 何国森 Preparation method of rhododendron culture medium
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CN104541838A (en) * 2014-09-30 2015-04-29 余大春 Azalea cuttage breeding technique
CN104541839A (en) * 2014-09-30 2015-04-29 余大春 Azalea wild-imitated cultivation technique
CN104542284A (en) * 2014-12-30 2015-04-29 北京林业大学 Tissue culture rapid propagation method for rhododendron irroratum
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CN106258959A (en) * 2016-08-08 2017-01-04 广州华苑园林股份有限公司 A kind of Folium Rhododendri Simsii quick breeding method for tissue culture
CN106258959B (en) * 2016-08-08 2018-10-23 广州华苑园林股份有限公司 A kind of azalea quick breeding method for tissue culture
CN107278686A (en) * 2017-06-06 2017-10-24 山东大丰园农业有限公司 A kind of American red-maple tissue culture outside sprout-cultivating-bottle radication agent and method for culturing seedlings
CN107182784A (en) * 2017-06-12 2017-09-22 云南省热带作物科学研究所 A kind of efficient acclimatization and transplantses method of rubber tree tissue-cultured seedling and rubber tree seedling culture method
CN107494264A (en) * 2017-08-31 2017-12-22 地缘(厦门)生物科技有限公司 A kind of method of Pterospermi Heterophylli tissue culture outside sprout-cultivating-bottle radication
CN108848977A (en) * 2017-12-20 2018-11-23 江苏省林业科学研究院 A kind of promotion blueberry tissue-cultured seedling rooting method
CN109997537A (en) * 2019-05-21 2019-07-12 黑龙江伊蓝生物科技有限公司 The micro- plug bottle ex vitro rooting technique of indigo fruit tissue culture subculture unrooted test tube seedling

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