CN104160965A - Rapid propagation method for litsea rotundifolia - Google Patents
Rapid propagation method for litsea rotundifolia Download PDFInfo
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- CN104160965A CN104160965A CN201410463083.8A CN201410463083A CN104160965A CN 104160965 A CN104160965 A CN 104160965A CN 201410463083 A CN201410463083 A CN 201410463083A CN 104160965 A CN104160965 A CN 104160965A
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- camphor tree
- jackal
- skin camphor
- jackal skin
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Abstract
The invention relates to a rapid propagation method for litsea rotundifolia. The rapid propagation method comprises the steps of disinfecting stems, inducing axillary buds, and performing rooting induction and performing acclimatization and transplanting. A litsea rotundifolia tissue culture method is easy to operate and the multiplication rate is high; the problem of limited wild resources and the difficulty in collection are solved; a large quantity of litsea rotundifolia seedlings can be obtained within short period; a basis is supplied for development of the litsea rotundifolia.
Description
Technical field
The present invention relates to the cultivation of jackal skin camphor tree under condition of tissue culture, belong to plant technology field.
Background technology
Jackal skin camphor tree, Lauraceae, evergreen shrubs or dungarunga,
litsea rotundifoliavar. oblongifolia, has another name called: large ash wood, young, the white bavin of blinds, fragrant spicebush bark or branchlet and leaf are distributed in Guangdong, Guangxi, Jiangxi, Hunan, Guizhou.Be born under mountain in shrub or sparse woods.The fatty oil of seed; Fruit leaf is containing aromatic oil; Root is containing alkaloid, phenols, amino acid; Leaf is containing glycosides displayed, phenols, amino acid, carbohydrate etc.Be a kind of traditional Chinese medicine, be used as medicine with root, dispel rheumatism, promoting qi circulation and relieving pain, activating blood to promote menstruation.For rheumatic arthritis, pain in waist and lower extremities, traumatic injury, dysmenorrhoea, stomachache, diarrhoea, oedema.The collection of jackal skin camphor tree at present adopts field to gather more, the difficulty of gathering, and tissue culture method can overcome this shortcoming, and simple to operate, and genetic stability is good, for the exploitation of jackal skin camphor tree provide good foundation.
Summary of the invention
Technical problem to be solved by this invention is to provide the method for quickly breeding of jackal skin camphor tree, and tissue culture method is easy and simple to handle, the rate of increase is high, overcomes wild resource limited, gathers difficulty, can obtain in a short time a large amount of jackal skin camphor tree seedling, for the exploitation of jackal skin camphor tree provides basis.
For solving the problems of the technologies described above, the present invention adopts following technical proposal:
Get jackal skin camphor tree stem with bud, in bleaching powder, soak 3min, flowing water rinses 40min, on superclean bench, 75% alcohol is processed 30s, clorox is processed 15min, aseptic water washing 5-7 time, in the jackal skin camphor tree stem with bud access improvement WS+IAA0.2mg/L+6-BA0.8mg/L+150g/L bananas juice medium that sterilization treatment is crossed, cultivate, additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature, the jackal skin camphor tree axillalry bud growing out, puts into proliferated culture medium improvement WS+IBA0.1mg/L+6-BA1mg/L+ sodium thiosulfate 100mg/L+0.2mg/LGA
3additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1500lx, 24 DEG C of temperature, the Multiple Buds that propagation cultivates is out put into root media 1/2MS+NAA0.5mg/L, additional saccharose 2%, agar 0.55%, PH5.8-5.9, illumination 2000lx, jackal skin camphor tree test-tube seedling transplanting after taking root carries out hardening cultivation to land for growing field crops, test-tube plantlet is lifted to sealed membrane, in culturing room, cultivate after one week and take out from blake bottle, wash away root medium, plant and cultivate in field, select hillside or terraced fields better, soil can not be cold soil, select sandy soil ground, deep ploughing, before planting, execute farmyard manure, after planting, use atomizers spray 0.2% carbendazim, cultivate after 20 days, statistics survival rate.
The jackal skin camphor tree fast growth that adopts the present invention to prepare, simple to operate, energy consumption is little, pollutes little.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiment.
Embodiment
Embodiment 1
Get jackal skin camphor tree stem with bud, in bleaching powder, soak 3min, flowing water rinses 40min, on superclean bench, 75% alcohol is processed 30s, clorox is processed 15min, aseptic water washing 5-7 time, in the jackal skin camphor tree stem with bud access improvement WS+IAA0.2mg/L+6-BA0.8mg/L+150g/L bananas juice medium that sterilization treatment is crossed, cultivate, additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature, the jackal skin camphor tree axillalry bud growing out, put into proliferated culture medium improvement WS+IBA0.05mg/L+6-BA0.5mg/L+ sodium thiosulfate 100mg/L+0.2mg/LGA
3additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1500lx, 24 DEG C of temperature, the Multiple Buds that propagation cultivates is out put into root media 1/4MS+NAA0.5mg/L, additional saccharose 2%, agar 0.55%, PH5.8-5.9, illumination 2000lx, jackal skin camphor tree test-tube seedling transplanting after taking root carries out hardening cultivation to land for growing field crops, test-tube plantlet is lifted to sealed membrane, in culturing room, cultivate after one week and take out from blake bottle, wash away root medium, plant and cultivate in field, select hillside or terraced fields better, soil can not be cold soil, select sandy soil ground, deep ploughing, before planting, execute farmyard manure, after planting, use atomizers spray 0.2% carbendazim, cultivate after 20 days, statistics survival rate.
Embodiment 2
Get jackal skin camphor tree stem with bud, in bleaching powder, soak 3min, flowing water rinses 40min, on superclean bench, 75% alcohol is processed 30s, clorox is processed 15min, aseptic water washing 5-7 time, in the jackal skin camphor tree stem with bud access improvement WS+IAA0.2mg/L+6-BA0.8mg/L+150g/L bananas juice medium that sterilization treatment is crossed, cultivate, additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature, the jackal skin camphor tree axillalry bud growing out, put into proliferated culture medium improvement WS+IBA0.15mg/L+6-BA1.0mg/L+ sodium thiosulfate 100mg/L+0.2mg/LGA
3additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1500lx, 24 DEG C of temperature, the Multiple Buds that propagation cultivates is out put into root media 3/4MS+NAA0.5mg/L, additional saccharose 2%, agar 0.55%, PH5.8-5.9, illumination 2000lx, jackal skin camphor tree test-tube seedling transplanting after taking root carries out hardening cultivation to land for growing field crops, test-tube plantlet is lifted to sealed membrane, in culturing room, cultivate after one week and take out from blake bottle, wash away root medium, plant and cultivate in field, select hillside or terraced fields better, soil can not be cold soil, select sandy soil ground, deep ploughing, before planting, execute farmyard manure, after planting, use atomizers spray 0.2% carbendazim, cultivate after 20 days, statistics survival rate.
Embodiment 3
Get jackal skin camphor tree stem with bud, in bleaching powder, soak 3min, flowing water rinses 40min, on superclean bench, 75% alcohol is processed 30s, clorox is processed 15min, aseptic water washing 5-7 time, in the jackal skin camphor tree stem with bud access improvement WS+IAA0.2mg/L+6-BA0.8mg/L+150g/L bananas juice medium that sterilization treatment is crossed, cultivate, additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature, the jackal skin camphor tree axillalry bud growing out, put into proliferated culture medium improvement WS+IBA0.1mg/L+6-BA0.5mg/L+ sodium thiosulfate 100mg/L+0.2mg/LGA
3additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1500lx, 24 DEG C of temperature, the Multiple Buds that propagation cultivates is out put into root media 1/2MS+NAA0.5mg/L, additional saccharose 2%, agar 0.55%, PH5.8-5.9, illumination 2000lx, jackal skin camphor tree test-tube seedling transplanting after taking root carries out hardening cultivation to land for growing field crops, test-tube plantlet is lifted to sealed membrane, in culturing room, cultivate after one week and take out from blake bottle, wash away root medium, plant and cultivate in field, select hillside or terraced fields better, soil can not be cold soil, select sandy soil ground, deep ploughing, before planting, execute farmyard manure, after planting, use atomizers spray 0.2% carbendazim, cultivate after 20 days, statistics survival rate.
Embodiment 4
Get jackal skin camphor tree stem with bud, in bleaching powder, soak 3min, flowing water rinses 40min, on superclean bench, 75% alcohol is processed 30s, clorox is processed 15min, aseptic water washing 5-7 time, in the jackal skin camphor tree stem with bud access improvement WS+IAA0.2mg/L+6-BA0.8mg/L+150g/L bananas juice medium that sterilization treatment is crossed, cultivate, additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature, the jackal skin camphor tree axillalry bud growing out, put into proliferated culture medium improvement WS+IBA0.1mg/L+6-BA1.0mg/L+ sodium thiosulfate 100mg/L+0.2mg/LGA
3additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1500lx, 24 DEG C of temperature, the Multiple Buds that propagation cultivates is out put into root media 3/4MS+NAA0.5mg/L, additional saccharose 2%, agar 0.55%, PH5.8-5.9, illumination 2000lx, jackal skin camphor tree test-tube seedling transplanting after taking root carries out hardening cultivation to land for growing field crops, test-tube plantlet is lifted to sealed membrane, in culturing room, cultivate after one week and take out from blake bottle, wash away root medium, plant and cultivate in field, select hillside or terraced fields better, soil can not be cold soil, select sandy soil ground, deep ploughing, before planting, execute farmyard manure, after planting, use atomizers spray 0.2% carbendazim, cultivate after 20 days, statistics survival rate.
Claims (6)
1. a method for quickly breeding for jackal skin camphor tree, comprises sterilization, the induction of Multiple Buds, the propagation of Multiple Buds, the culture of rootage of axillalry bud, and its key step is as follows:
(1) get the stem section of jackal skin camphor tree with axillalry bud, it is carried out to routine disinfection processing;
(2) in the jackal skin camphor tree stem with bud access improvement WS+IAA0.2mg/L+6-BA0.8mg/L+150g/L bananas juice medium of step (1) sterilization treatment being crossed, cultivate additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1000lx, 24 DEG C of temperature;
(3) get the jackal skin camphor tree axillalry bud that step (2) grows out, put into proliferated culture medium improvement WS+IBA0.05-0.15mg/L+6-BA0.5-1.0mg/L+ sodium thiosulfate 100mg/L+0.2mg/LGA
3additional saccharose 3%, agar 0.6%, PH5.8-5.9, illumination 1500lx, 24 DEG C of temperature;
(4) getting step (3) breeds the Multiple Buds of cultivating out and puts into root media 1/4-3/4MS+NAA0.5mg/L, additional saccharose 2%, agar 0.55%, PH5.8-5.9, illumination 2000lx;
(5) the jackal skin camphor tree test-tube seedling transplanting of getting after step (4) is taken root carries out hardening cultivation to land for growing field crops.
2. according to the method for quickly breeding of a kind of jackal skin camphor tree claimed in claim 1, it is characterized in that: the sterilization treatment of the stem with bud of jackal skin camphor tree described in step (1) is: get jackal skin camphor tree stem with bud, in bleaching powder, soak 3min, flowing water rinses 40min, on superclean bench, 75% alcohol is processed 30s, clorox is processed 15min, aseptic water washing 5-7 time.
3. according to the method for quickly breeding of a kind of jackal skin camphor tree claimed in claim 1, it is characterized in that: the induction of step (2) axillalry bud has added 150g/L bananas juice in cultivating, and contributes to the differentiation of indefinite bud.
4. according to the method for quickly breeding of a kind of jackal skin camphor tree claimed in claim 1, it is characterized in that: in the proliferated culture medium in step (3), added sodium thiosulfate 100mg/L, can prevent the generation of browning.
5. according to the method for quickly breeding of a kind of jackal skin camphor tree claimed in claim 1, it is characterized in that: 1/4,1/2,3/4 pair of induction that jackal skin camphor tree takes root of screening in step (4).
6. according to the method for quickly breeding of a kind of jackal skin camphor tree claimed in claim 1, it is characterized in that: in step (5), the hardening cultural method of jackal skin camphor tree, for test-tube plantlet is lifted to sealed membrane, is cultivated after one week and taken out from blake bottle in culturing room, wash away root medium, plant and cultivate in field, select hillside or terraced fields better, soil can not be cold soil, select sandy soil ground, deep ploughing, executes farmyard manure before planting, and uses atomizers spray 0.2% carbendazim after planting, cultivate after 20 days statistics survival rate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410463083.8A CN104160965A (en) | 2014-09-12 | 2014-09-12 | Rapid propagation method for litsea rotundifolia |
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| CN201410463083.8A CN104160965A (en) | 2014-09-12 | 2014-09-12 | Rapid propagation method for litsea rotundifolia |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409779A (en) * | 2015-12-25 | 2016-03-23 | 鲁东大学 | Tissue culture rapid reproduction method for Cinnamomum kanehirae |
| CN110622861A (en) * | 2019-10-15 | 2019-12-31 | 重庆市林业科学研究院 | Sterilization method for improving tissue culture starting rate of cinnamomum camphora explants |
-
2014
- 2014-09-12 CN CN201410463083.8A patent/CN104160965A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409779A (en) * | 2015-12-25 | 2016-03-23 | 鲁东大学 | Tissue culture rapid reproduction method for Cinnamomum kanehirae |
| CN105409779B (en) * | 2015-12-25 | 2017-11-24 | 鲁东大学 | The method of cinnamomum kanehirai tissue-culturing quick-propagation |
| CN110622861A (en) * | 2019-10-15 | 2019-12-31 | 重庆市林业科学研究院 | Sterilization method for improving tissue culture starting rate of cinnamomum camphora explants |
| CN110622861B (en) * | 2019-10-15 | 2021-04-13 | 重庆市林业科学研究院 | Sterilization method for improving tissue culture starting rate of cinnamomum camphora explants |
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