CN108719064A - A kind of hall crabapple flower rapid propagation method - Google Patents
A kind of hall crabapple flower rapid propagation method Download PDFInfo
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- CN108719064A CN108719064A CN201810486935.3A CN201810486935A CN108719064A CN 108719064 A CN108719064 A CN 108719064A CN 201810486935 A CN201810486935 A CN 201810486935A CN 108719064 A CN108719064 A CN 108719064A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/27—Pulp, e.g. bagasse
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
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- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of hall crabapple flower rapid propagation method, this method step includes that Initial culture, squamous subculture, culture of rootage and seedling practice transplantation of seedlings.The hall crabapple flower that culture medium provided by the present invention cultivates; planting cost is low; investment risk is small, and survival rate is high after transplanting, and the growing way of plant is strong; the period of cultivation is short; technological applicability is strong, suitable for being widely applied plantation, to improve manual scale planting, the enhancing product competitiveness of hall crabapple flower; it haves laid a good foundation, with good economic efficiency and social benefit.
Description
Technical field
The invention belongs to growing and cultivation technical fields, and in particular to a kind of hall crabapple flower rapid propagation method.
Background technology
Hall crabapple flower is rose family Malus deciduous tree, is important ornamental tree species, and there are a cultivation in various regions, lightly seasoned,
Hardship, mild-natured, flower is used as medicine, and has the effect of regulating menstruation and activating blood, is chiefly used in treating haematemesis, hematuria, have blood in stool, traumatic injury, fracture etc.
Disease, dying Malus spectabilis also have certain edible function, its fruit is sour-sweet edible, can be made into preserved fruit, in food, health products
Dosage is very big, and especially interior people's life substantially improves in recent years, and the demand of hall crabapple flower is also constantly increasing, usually
In the case of, the method for breeding hall crabapple flower mainly has the methods of a plant division, grafting, press strip, cuttage, plant division be between March in spring from
The seedling is sprouted beside maternal plant rhizosphere gently separate and transplanted, bred using the method for plant division and grafting, survival rate
It is relatively low, and the maternal plant needed and stock quantity are more, and after transplanting or grafting, the individual difference of hall crabapple flower is larger, survival rate
It is low, meanwhile, the time of cultivation is longer.
Invention content
The purpose of the present invention is to provide a kind of hall crabapple flower rapid propagation method, this method is suitble to hall crabapple flower explant
It is put into culture, the planting percent of hall crabapple flower can be greatly improved.
In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
A kind of hall crabapple flower rapid propagation method, includes the following steps：
（1）The terminal bud cut alum is impregnated 10 min, is then rushed using clear water by the terminal bud for cutting the raw healthy and strong branches of 3-4
It washes, the terminal bud after flushing dries in the shade naturally, then with hydrogen peroxide dipping 2-3 min, and 75% medicinal alcohol rinses 20 s, finally with secondary
Distill water wash；Terminal bud after elution is placed in Initial culture base, after cultivating 15-20 days, obtains hall crabapple flower terminal bud callus
Tissue；The Initial culture base is：Using MS as minimal medium, 0.1-2.0 mg/L 6- benzyl purines, 0.1-1.0 is added
Mg/L zeatin, 0.5-3.0 g/L gibberellin, 0.5-3.0 mg/L ammonium hydroxide, 30-50 g/L glucose and 3.0-5.0 g/L fine jades
Fat, the pH value for adjusting inducing culture are 5.8-6.2；
（2）Hall crabapple flower terminal bud callus is placed in subculture medium, is 20 ± 2 DEG C in temperature, 2500 Lux items of illumination
After being cultivated 30-35 days under part, callus is differentiated to form seedling of growing thickly, and culture medium is：30 g/L of Nitsch+2 mgB5+ sucrose；
（3）It will grow thickly in small transplantation of seedlings to root media, after cultivating 20-30 days, the white of 1-2 centimeter lengths is grown on root restriction
Root, is transferred under natural light irradiation culture 25-30 days, and the more sturdy hall crabapple flower of acquisition is taken root seedling；The culture of rootage
Base is：0.8-3.0 g/L indolebutyric acids, 1.0-5.0 mg/L naphthalene second is added in 1/2MS+IBA0.5 mg/L+NAA0.1 mg/L
Acid, 0.5-2.0 g/L gibberellin, 2-5% sucrose and 0.2-0.5% activated carbons；
（4）Hall crabapple flower seedling of taking root is transplanted, after planting seedbed, is poured with clear water, weeding of periodically digging.
Further, step（4）Described in medium of seedling bed be：The decomposed rice husks of 5-20, the decomposed bagasse of 5-20,10-15
Malus spectabilis leaf, 35-40 part vermiculite and 10-25 parts of perlites.
Further, the step（1）Extremely（3）In, condition of culture of the hall crabapple flower in culture medium is：Cultivation temperature
It it is 25-30 DEG C, intensity of illumination is 2000-3000 Lux, and light application time is 10-12 h/d.
The invention has the advantages that：
Method for tissue culture breeding provided by the invention is more easy, can obtain a large amount of aseptic seedling, breeding week in a short time
Phase shortens, so as to rapid expansion cultivated area, greatly accelerate the reproduction speed of hall crabapple flower, effective solution cuttage
The low problem of survival rate；And survival rate is high after transplanting, the growing way of plant is strong, and the period of cultivation is short.
Specific implementation mode
To make the objectives, technical solutions, and advantages of the present invention clearer, with reference to specific embodiment to this hair
Bright technical solution is described in further detail.
Embodiment 1
A kind of hall crabapple flower rapid propagation method, includes the following steps：
（1）The terminal bud cut alum is impregnated 10 min, is then rushed using clear water by the terminal bud for cutting the raw healthy and strong branches of 3-4
It washes, the terminal bud after flushing dries in the shade naturally, then with 2 min of hydrogen peroxide dipping, and 75% medicinal alcohol rinses 20 s, finally with secondary steaming
Distilled water elutes；Terminal bud after elution is placed in Initial culture base, after cultivating 15 days, obtains hall crabapple flower terminal bud callus；
The Initial culture base is：Using MS as minimal medium, 0.1 mg/L 6- benzyl purines, 0.1 mg/L zeatin, 0.5 is added
G/L gibberellin, 0.5 mg/L ammonium hydroxide, 30 g/L glucose and 3.0 g/L agar, the pH value for adjusting inducing culture are 5.8-
6.2；Condition of culture is：Cultivation temperature is 25 DEG C, and intensity of illumination is 2000 Lux, and light application time is 10 h/d.
（2）Hall crabapple flower terminal bud callus is placed in subculture medium, is 20 ± 2 DEG C in temperature, illumination 2500
After being cultivated 30 days under the conditions of Lux, callus is differentiated to form seedling of growing thickly, and culture medium is：30 g/ of Nitsch+2 mgB5+ sucrose
L；Condition of culture is：Cultivation temperature is 25 DEG C, and intensity of illumination is 2000 Lux, and light application time is 10 h/d.
（3）It will grow thickly in small transplantation of seedlings to root media, after cultivating 20 days, the white of 1-2 centimeter lengths is grown on root restriction
Color root is transferred under natural light irradiation culture 25 days, obtains more sturdy hall crabapple flower and takes root seedling；The root media
For：It is red that 0.8 g/L indolebutyric acids, 1.0 mg/L methyl α-naphthyl acetates, 0.5 g/L is added in 1/2MS+IBA0.5 mg/L+NAA0.1 mg/L
Mycin, 2% sucrose and 0.2% activated carbon；Condition of culture is：Cultivation temperature is 25 DEG C, and intensity of illumination is 2000 Lux, illumination
Time is 10 h/d.
（4）Hall crabapple flower seedling of taking root is transplanted, after planting seedbed, is poured with clear water, weeding of periodically digging.Seedbed
Matrix is：5 decomposed rice husks, 20 decomposed bagasse, 15 Malus spectabilis leaves, 40 parts of vermiculites and 20 parts of perlites.
Embodiment 2
A kind of hall crabapple flower rapid propagation method, includes the following steps：
（1）The terminal bud cut alum is impregnated 10 min, is then rushed using clear water by the terminal bud for cutting the raw healthy and strong branches of 3-4
It washes, the terminal bud after flushing dries in the shade naturally, then with 3 min of hydrogen peroxide dipping, and 75% medicinal alcohol rinses 20 s, finally with secondary steaming
Distilled water elutes；Terminal bud after elution is placed in Initial culture base, after cultivating 20 days, obtains hall crabapple flower terminal bud callus；
The Initial culture base is：Using MS as minimal medium, 2.0 mg/L 6- benzyl purines, 1.0 mg/L zeatin, 3.0 are added
G/L gibberellin, 3.0 mg/L ammonium hydroxide, 50 g/L glucose and 5.0 g/L agar, the pH value for adjusting inducing culture are 5.8-
6.2；Condition of culture is：Cultivation temperature is 30 DEG C, and intensity of illumination is 3000 Lux, and light application time is 12 h/d.
（2）Hall crabapple flower terminal bud callus is placed in subculture medium, is 20 ± 2 DEG C in temperature, illumination 2500
After being cultivated 35 days under the conditions of Lux, callus is differentiated to form seedling of growing thickly, and culture medium is：30 g/ of Nitsch+2 mgB5+ sucrose
L；Condition of culture is：Cultivation temperature is 30 DEG C, and intensity of illumination is 3000 Lux, and light application time is 12 h/d.
（3）It will grow thickly in small transplantation of seedlings to root media, after cultivating 30 days, the white of 1-2 centimeter lengths is grown on root restriction
Color root is transferred under natural light irradiation culture 30 days, obtains more sturdy hall crabapple flower and takes root seedling；The root media
For：It is red that 3.0 g/L indolebutyric acids, 5.0 mg/L methyl α-naphthyl acetates, 2.0 g/L are added in 1/2MS+IBA0.5 mg/L+NAA0.1 mg/L
Mycin, 5% sucrose and 0.5% activated carbon；Condition of culture is：Cultivation temperature is 30 DEG C, and intensity of illumination is 3000 Lux, illumination
Time is 12 h/d.
（4）Hall crabapple flower seedling of taking root is transplanted, after planting seedbed, is poured with clear water, weeding of periodically digging.Seedbed
Matrix is：20 decomposed rice husks, 5 decomposed bagasse, 10 Malus spectabilis leaves, 40 parts of vermiculites and 25 parts of perlites.
Embodiment 3
A kind of hall crabapple flower rapid propagation method, includes the following steps：
（1）The terminal bud cut alum is impregnated 10 min, is then rushed using clear water by the terminal bud for cutting the raw healthy and strong branches of 3-4
It washes, the terminal bud after flushing dries in the shade naturally, then with 2.5 min of hydrogen peroxide dipping, and 75% medicinal alcohol rinses 20 s, finally with secondary
Distill water wash；Terminal bud after elution is placed in Initial culture base, after cultivating 17 days, obtains hall crabapple flower terminal bud callus group
It knits；The Initial culture base is：Using MS as minimal medium, be added 1.0 mg/L 6- benzyl purines, 0.6 mg/L zeatin,
2.0 g/L gibberellin, 2.0 mg/L ammonium hydroxide, 40 g/L glucose and 4.0 g/L agar, the pH value for adjusting inducing culture are
5.8-6.2；Condition of culture is：Cultivation temperature is 27 DEG C, and intensity of illumination is 2500 Lux, and light application time is 11 h/d.
（2）Hall crabapple flower terminal bud callus is placed in subculture medium, is 20 ± 2 DEG C in temperature, illumination 2500
After being cultivated 33 days under the conditions of Lux, callus is differentiated to form seedling of growing thickly, and culture medium is：30 g/ of Nitsch+2 mgB5+ sucrose
L；Condition of culture is：Cultivation temperature is 26 DEG C, and intensity of illumination is 2800 Lux, and light application time is 11 h/d.
（3）It will grow thickly in small transplantation of seedlings to root media, after cultivating 24 days, the white of 1-2 centimeter lengths is grown on root restriction
Color root is transferred under natural light irradiation culture 28 days, obtains more sturdy hall crabapple flower and takes root seedling；The root media
For：It is red that 2.0 g/L indolebutyric acids, 3.0 mg/L methyl α-naphthyl acetates, 1.0 g/L are added in 1/2MS+IBA0.5 mg/L+NAA0.1 mg/L
Mycin, 4% sucrose and 0.4% activated carbon；Condition of culture is：Cultivation temperature is 27 DEG C, and intensity of illumination is 2600 Lux, illumination
Time is 11 h/d.
（4）Hall crabapple flower seedling of taking root is transplanted, after planting seedbed, is poured with clear water, weeding of periodically digging.Seedbed
Matrix is：15 decomposed rice husks, 15 decomposed bagasse, 13 Malus spectabilis leaves, 37 parts of vermiculites and 20 parts of perlites.
Embodiment 4
A kind of hall crabapple flower rapid propagation method, includes the following steps：
（1）The terminal bud cut alum is impregnated 10 min, is then rushed using clear water by the terminal bud for cutting the raw healthy and strong branches of 3-4
It washes, the terminal bud after flushing dries in the shade naturally, then with 2 min of hydrogen peroxide dipping, and 75% medicinal alcohol rinses 20 s, finally with secondary steaming
Distilled water elutes；Terminal bud after elution is placed in Initial culture base, after cultivating 19 days, obtains hall crabapple flower terminal bud callus；
The Initial culture base is：Using MS as minimal medium, 1.2 mg/L 6- benzyl purines, 0.7 mg/L zeatin, 2.3 are added
G/L gibberellin, 1.3 mg/L ammonium hydroxide, 35 g/L glucose and 3.5 g/L agar, the pH value for adjusting inducing culture are 5.8-
6.2；Condition of culture is：Cultivation temperature is 29 DEG C, and intensity of illumination is 2800 Lux, and light application time is 10 h/d.
（2）Hall crabapple flower terminal bud callus is placed in subculture medium, is 20 ± 2 DEG C in temperature, illumination 2500
After being cultivated 30-35 days under the conditions of Lux, callus is differentiated to form seedling of growing thickly, and culture medium is：Nitsch+2 mgB5+ sucrose 30
g/L；Condition of culture is：Cultivation temperature is 28 DEG C, and intensity of illumination is 2600 Lux, and light application time is 12 h/d.
（3）It will grow thickly in small transplantation of seedlings to root media, after cultivating 27 days, the white of 1-2 centimeter lengths is grown on root restriction
Color root is transferred under natural light irradiation culture 27 days, obtains more sturdy hall crabapple flower and takes root seedling；The root media
For：It is red that 1.3 g/L indolebutyric acids, 1.5 mg/L methyl α-naphthyl acetates, 1.2 g/L are added in 1/2MS+IBA0.5 mg/L+NAA0.1 mg/L
Mycin, 3% sucrose and 0.5% activated carbon；Condition of culture is：Cultivation temperature is 28 DEG C, and intensity of illumination is 2300 Lux, illumination
Time is 12 h/d.
（4）Hall crabapple flower seedling of taking root is transplanted, after planting seedbed, is poured with clear water, weeding of periodically digging.Seedbed
Matrix is：20 decomposed rice husks, 20 decomposed bagasse, 15 Malus spectabilis leaves, 35 parts of vermiculites and 10 parts of perlites.
Claims (3)
1. a kind of hall crabapple flower rapid propagation method, which is characterized in that include the following steps：
（1）The terminal bud cut alum is impregnated 10 min, is then rushed using clear water by the terminal bud for cutting the raw healthy and strong branches of 3-4
It washes, the terminal bud after flushing dries in the shade naturally, then with hydrogen peroxide dipping 2-3 min, and 75% medicinal alcohol rinses 20 s, finally with secondary
Distill water wash；Terminal bud after elution is placed in Initial culture base, after cultivating 15-20 days, obtains hall crabapple flower terminal bud callus
Tissue；The Initial culture base is：Using MS as minimal medium, 0.1-2.0 mg/L 6- benzyl purines, 0.1-1.0 is added
Mg/L zeatin, 0.5-3.0 g/L gibberellin, 0.5-3.0 mg/L ammonium hydroxide, 30-50 g/L glucose and 3.0-5.0 g/L fine jades
Fat, the pH value for adjusting inducing culture are 5.8-6.2；
（2）Hall crabapple flower terminal bud callus is placed in subculture medium, is 20 ± 2 DEG C in temperature, 2500 Lux items of illumination
After being cultivated 30-35 days under part, callus is differentiated to form seedling of growing thickly, and culture medium is：30 g/L of Nitsch+2 mgB5+ sucrose；
（3）It will grow thickly in small transplantation of seedlings to root media, after cultivating 20-30 days, the white of 1-2 centimeter lengths is grown on root restriction
Root, is transferred under natural light irradiation culture 25-30 days, and the more sturdy hall crabapple flower of acquisition is taken root seedling；The culture of rootage
Base is：0.8-3.0 g/L indolebutyric acids, 1.0-5.0 mg/L naphthalene second is added in 1/2MS+IBA0.5 mg/L+NAA0.1 mg/L
Acid, 0.5-2.0 g/L gibberellin, 2-5% sucrose and 0.2-0.5% activated carbons；
（4）Hall crabapple flower seedling of taking root is transplanted, after planting seedbed, is poured with clear water, weeding of periodically digging.
2. a kind of hall crabapple flower rapid propagation method according to claim 1, which is characterized in that step（4）Described in
Medium of seedling bed is：The decomposed rice husks of 5-20, the decomposed bagasse of 5-20,10-15 Malus spectabilis leaf, 35-40 parts of vermiculites and 10-25 parts of pearls
Rock.
3. a kind of hall crabapple flower rapid propagation method according to claim 2, which is characterized in that the step（1）Extremely（3）
In, condition of culture of the hall crabapple flower in culture medium is：Cultivation temperature is 25-30 DEG C, intensity of illumination 2000-3000
Lux, light application time are 10-12 h/d.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110495339A (en) * | 2019-09-23 | 2019-11-26 | 成都环美园林生态股份有限公司 | A kind of hall crabapple flower method for planting on the above plateau of four km of height above sea level |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392597A (en) * | 2013-07-24 | 2013-11-20 | 江苏绿苑园林建设有限公司 | Tissue culture method of North American begonia |
| CN104686331A (en) * | 2015-02-21 | 2015-06-10 | 杨业云 | Tissue culture and rapid propagation method for malus halliana |
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2018
- 2018-05-21 CN CN201810486935.3A patent/CN108719064A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392597A (en) * | 2013-07-24 | 2013-11-20 | 江苏绿苑园林建设有限公司 | Tissue culture method of North American begonia |
| CN104686331A (en) * | 2015-02-21 | 2015-06-10 | 杨业云 | Tissue culture and rapid propagation method for malus halliana |
Non-Patent Citations (1)
| Title |
|---|
| 张庆田等: "垂丝海棠组培再生体系建立的研究", 《生物技术》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110495339A (en) * | 2019-09-23 | 2019-11-26 | 成都环美园林生态股份有限公司 | A kind of hall crabapple flower method for planting on the above plateau of four km of height above sea level |
| CN110495339B (en) * | 2019-09-23 | 2022-04-08 | 成都环美园林生态股份有限公司 | Malus halliana planting method at altitude of more than four kilometers |
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Application publication date: 20181102 |