CN105519434A - A tissue cultured seedling micrografting method for acer palmatum 'orange dream' - Google Patents
A tissue cultured seedling micrografting method for acer palmatum 'orange dream' Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
Abstract
A tissue cultured seedling micrografting method for acer palmatum 'orange dream' is disclosed. The method includes (1) a step of culturing a sterile stock, namely a step of cutting an acer elegantulum branch segment with sprouts, performing tissue culture to obtain an acer elegantulum sterile seedling, and cutting the acer elegantulum sterile seedling in an inclined matter to obtain the sterile stock, (2) a step of culturing a sterile scion, namely a step of cutting an acer palmatum 'orange dream' branch segment with sprouts, performing tissue culture to obtain an acer palmatum 'orange dream' sterile seedling, and cutting the acer palmatum 'orange dream' sterile seedling in an inclined matter to obtain the sterile scion, (3) a step of tissue cultured seedling micrografting, namely a step of aligning the oblique section of the sterile stock and the oblique section of the sterile scion, fixing to obtain a micrografted seedling, inoculating the micrografted seedling to a medium and culturing, and (4) a step of seedling hardening and transplanting, namely a step of transferring the micrografted seedling obtained in the step (3) into a greenhouse, performing earlier-period seedling hardening, transferring into a seed bed, performing seedling hardening, performing mist spray treatment, sterilizing, and transplanting after the seedling hardening is completed. The method increases the grafting efficiency, increases the reproductive output, is high in survival rate and can be used for large-scale cultivation of small-size acer palmatum 'orange dream' seedlings.
Description
Technical field
The present invention relates to propagation by grafiting technical field, be specifically related to a kind of plantlet in vitro micro-grafting method of dream of Japanese maple orange.
Background technology
The ornamental value of the dream (Acerpalmatum " OrangeDream ") of Japan's maple orange is high, and kind is unique, and spring, leaf look greenish-yellow, more by solar radiation, leaf look more becomes yellow, and limb is general red, is orange gradually, become zingy orange to summer, vein is yellow, and autumn is golden yellow, and three Ji Yese changes obviously, and all the time burnt phenomenon under burning sun, be received by the market very much.
At present, the dream of Japanese maple orange is mainly through traditional grafting method, and be subject to seasonal restrictions, reproductive number is few, and survival rate is low, the defect that cost of labor is higher, and stock specification minimum be about 1cm, small dimension grafting can not be produced, micro-potted plant needs can not be met.
Summary of the invention
In view of this, the application provides a kind of plantlet in vitro micro-grafting method of dream of Japanese maple orange, and described method can improve the efficiency of grafting, improve reproductive number, environment is controlled, and survival rate is high, and grafting cultivation period is short, can not be subject to seasonal restrictions, overcome traditional grafting method to be subject to seasonal restrictions, reproductive number is few, and survival rate is low, the defect that cost of labor is higher, can carry out large-scale industrialization nursery and deep processing.
For solving above technical problem, technical scheme provided by the invention is a kind of plantlet in vitro micro-grafting method of dream of Japanese maple orange, said method comprising the steps of:
(1) aseptic stock is cultivated: cut Fructus Aceris elegantulis band bud branch section, carry out tissue cultures, obtain Fructus Aceris elegantulis aseptic seedling, Fructus Aceris elegantulis aseptic seedling cut sth. askew and block, obtain aseptic stock;
(2) aseptic scion cultivated: the dream band bud branch section cutting Japanese maple orange, carries out tissue cultures, obtains the dream aseptic seedling of Japanese maple orange, the dream aseptic seedling of Japanese maple orange cut sth. askew and block, obtain aseptic scion;
(3) plantlet in vitro micrografting: is alignd in the oblique section of the oblique section of the aseptic stock described in step (1) with the described aseptic scion of step (2), and it is fixing, obtain micrografting seedling, described grafting is inoculated on medium and cultivated for 4-5 weeks, consisting of of described medium: MS minimal medium, supplements 0.25-1.50mg/L6-BA, 0.02-0.10mg/LNAA, 5-30g/L sucrose;
(4) acclimatization and transplants: step (3) gained micrografting seedling is proceeded to greenhouse initial stage hardening 5-8 days, then proceed to hardening 4-5 week in seedbed, in described seedbed during hardening, carry out spraying process and sterilization processing, can transplant after hardening terminates.
Preferably, in described plantlet in vitro micrografting, the condition that micrografting seedling is cultivated in the medium is: temperature is 23-27 DEG C, and intensity of illumination is 2300-2600Lx, and light application time is 12h/d.
Preferably, in described plantlet in vitro micrografting, consisting of of described medium: MS minimal medium, supplements 0.5-1.25mg/L6-BA, 0.03-0.07mg/LNAA, 10-25g/L sucrose.
More preferred, in described plantlet in vitro micrografting, consisting of of described medium: MS minimal medium, supplements 1.0mg/L6-BA, 0.05mg/LNAA, 20g/L sucrose.
Preferably, in described plantlet in vitro micrografting, also supplement 3-7g/L agar in described medium, the pH value of described agar is 6.5.
Preferably, the stem of described aseptic stock is slightly more than 1.5mm, and the stem of described aseptic scion is slightly more than 1mm.
Preferably, in described acclimatization and transplants, the matrix in described seedbed is volume ratio is 2:(2-4) perlite and the mixture of humus.
Preferably, in described acclimatization and transplants, the method for described spraying process is: during fine day, at interval of 10-15min spraying, 3-4s; When rainy day or cloudy day, at interval of 18-25min spraying, 3-4s, the time of spraying process is 6-12 days.
Preferably, in described acclimatization and transplants, described bactericidal treatments is: used microbicide solution to spray grafting every 5-8 days comprehensively.
More preferred, described microbicide solution is selected from 70% thiophanate methyl, 800 times of solution, 50% carbendazim, 800 times of solution, 75% tpn, 800 times of solution for any one.
Wherein, during described aseptic stock is cultivated, the method for described tissue cultures is: cultivate being seeded on axillary bud deriving medium after Fructus Aceris elegantulis band bud branch section sterilization; The axillalry bud of sprouting is cut, is seeded on inducing clumping bud medium and cultivates; The Multiple Buds differentiated is cut into individual plant, and inoculation root media is cultivated, and obtains Fructus Aceris elegantulis aseptic seedling.
In described aseptic scion cultivated, the method for described tissue cultures is: cultivate being seeded on axillary bud deriving medium after the dream band bud branch section sterilization of Japanese maple orange; The axillalry bud of sprouting is cut, is seeded on inducing clumping bud medium and cultivates; The Multiple Buds differentiated is cut into individual plant, and inoculation root media is cultivated, and obtains the dream aseptic seedling of Japanese maple orange.
MS minimal medium described in the application has higher inorganic salt concentration, the mineral nutrition needed for tissue growth can be ensured, the growth of callus can also be accelerated, more stable ionic equilibrium solution, its nitrate content is high, and quantity and the ratio of its nutrient are suitable, can meet nutrition and the physiological requirements of plant cell, thus the scope of application is relatively wider, and most plants tissue-culturing quick-propagation uses it as the minimal medium of medium.
Described 6-BA is 6-benzyl aminoadenine, it is a kind of auxin, its Main Function is the formation promoting bud, also can occur by evoked callus, promote cell division, promote the differentiation of undifferentiated tissue, promote the accumulation of biological substance in vivo, promote that lateral bud occurs, preventing aging, is the basic element of cell division in plant tissue and cell culture.
Described NAA is methyl α-naphthyl acetate, it is a kind of auxin, use during cuttage breeding plant and use, also can be used for Plant Tissue Breeding, cell division and expansion can be promoted, induced synthesis adventive root increases setting, prevent shedding, change female, male flower ratio etc., can through the tender epidermis of blade, branch, seed enters in plant, with nutritional flow transporting to complete stool.
The application compared with prior art, it is described in detail as follows: technical scheme provides a kind of plantlet in vitro micro-grafting method of dream of Japanese maple orange, comprise aseptic stock to cultivate, aseptic scion cultivated, plantlet in vitro micrografting, the step of acclimatization and transplants, by in plantlet in vitro micrografting step, the screening of medium, obtain best nutrient media components and proportioning, adopt the medium that afore-mentioned plants somatotropin proportioning is formed, formula is simple, culture medium cost is low, the healing of micrografting wound can be accelerated, ensure that the survival rate of micrografting is more than 90%, thus improve the efficiency of grafting, improve reproductive number, because its environment is controlled, survival rate is high, grafting cultivation period is short, can not be subject to seasonal restrictions, therefore overcome traditional grafting method to be subject to seasonal restrictions, reproductive number is few, survival rate is low, the defect that cost of labor is higher, micro-potted landscape can be used for by the less grafting of production specification, micro-potted plant, the training of solution group is taken root difficult problem, large-scale industrialization nursery and deep processing can be carried out, there is good application prospect.
Embodiment
In order to make those skilled in the art understand technical scheme of the present invention better, below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, comprises the following steps:
(1) aseptic stock is cultivated: cut Fructus Aceris elegantulis band bud branch section, be seeded on axillary bud deriving medium and cultivate after sterilization; The axillalry bud of sprouting is cut, is seeded on inducing clumping bud medium and cultivates; The Multiple Buds differentiated is cut into individual plant, and inoculation root media is cultivated, and obtain Fructus Aceris elegantulis aseptic seedling, Fructus Aceris elegantulis aseptic seedling cut sth. askew and block, obtain aseptic stock, stem is slightly more than 1.5mm;
(2) aseptic scion cultivated: the dream band bud branch section cutting Japanese maple orange, is seeded to after sterilization on axillary bud deriving medium and cultivates; The axillalry bud of sprouting is cut, is seeded on inducing clumping bud medium and cultivates; The Multiple Buds differentiated is cut into individual plant, and inoculation root media is cultivated, and obtain the dream aseptic seedling of Japanese maple orange, the dream aseptic seedling of Japanese maple orange cut sth. askew and block, obtain aseptic scion, stem is slightly more than 1mm;
(3) plantlet in vitro micrografting: is alignd in the oblique section of the oblique section of the aseptic stock described in step (1) with the described aseptic scion of step (2), and adopt sterile milk sebific duct to fix, obtain micrografting seedling, described grafting is inoculated on medium and cultivated for 4-5 weeks, cultivation temperature is 23-27 DEG C, intensity of illumination is 2300-2600Lx, and light application time is 12h/d.Consisting of of described medium: MS minimal medium, supplement 1.0mg/L6-BA, 0.05mg/LNAA, 20g/L sucrose, 5g/L agar, wherein the pH value of agar is 6.5;
(4) acclimatization and transplants: step (3) gained micrografting seedling is proceeded to greenhouse initial stage hardening 7 days, proceed to hardening 4-5 week in seedbed again, seedbed was carried out disinfection in 7 days in advance, the matrix in seedbed is volume ratio is the perlite of 2:3 and the mixture of humus, in described seedbed during hardening, carry out spraying process and sterilization processing, can transplant after hardening terminates, the method of wherein said spraying process is: during fine day, at interval of 10min spraying 3s; When rainy day or cloudy day, at interval of 20min spraying 3s, the time of spraying process is 10 days, and described bactericidal treatments is: used microbicide solution to spray grafting every 7 days, described microbicide solution is 70% thiophanate methyl, 800 times of solution comprehensively.
Embodiment 2
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (3) plantlet in vitro micrografting, consisting of of described medium: MS minimal medium, supplements 1.0mg/L6-BA, 0.05mg/LNAA, 20g/L sucrose, 4g/L agar.
Embodiment 3
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (3) plantlet in vitro micrografting, consisting of of described medium: MS minimal medium, supplements 0.5mg/L6-BA, 0.03mg/LNAA, 10g/L sucrose, 4g/L agar.
Embodiment 4
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (3) plantlet in vitro micrografting, consisting of of described medium: MS minimal medium, supplements 1.25mg/L6-BA, 0.07mg/LNAA, 25g/L sucrose, 5g/L agar.
Embodiment 5
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (3) plantlet in vitro micrografting, consisting of of described medium: MS minimal medium, supplements 0.25mg/L6-BA, 0.02mg/LNAA, 5g/L sucrose, 4g/L agar.
Embodiment 6
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (3) plantlet in vitro micrografting, consisting of of described medium: MS minimal medium, supplements 1.50mg/L6-BA, 0.10mg/LNAA, 30g/L sucrose, 5g/L agar.
Embodiment 7
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (3) plantlet in vitro micrografting, MS minimal medium, supplements 0.1mg/L6-BA, 0.08mg/LNAA, 20g/L sucrose, 5g/L agar.
Embodiment 8
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (3) plantlet in vitro micrografting, MS minimal medium, supplements 0.1mg/L6-BA, 0.1mg/LNAA, 15g/L sucrose, 5g/L agar.
Embodiment 9
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (3) plantlet in vitro micrografting, MS minimal medium, supplements MS minimal medium, supplements 0.6mg/L6-BA, 0.06mg/LNAA, 20g/L sucrose, 5g/L agar.
Embodiment 10
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (3) plantlet in vitro micrografting, MS minimal medium, supplements MS minimal medium, supplements 0.6mg/L6-BA, 0.05mg/LNAA, 15g/L sucrose, 5g/L agar.
Embodiment 11
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (4) acclimatization and transplants, step (3) gained micrografting seedling is proceeded to greenhouse initial stage hardening 6 days, proceed to hardening 4-5 week in seedbed again, within 7 days, carry out disinfection to seedbed in advance, the matrix in seedbed is volume ratio is the perlite of 2:2 and the mixture of humus, in described seedbed during hardening, carry out spraying process and sterilization processing, can transplant after hardening terminates, the method for wherein said spraying process is: during fine day, at interval of 10min spraying 4s; When rainy day or cloudy day, at interval of 18min spraying 3s, the time of spraying process is 9 days, and described bactericidal treatments is: used microbicide solution to spray grafting every 6 days, described microbicide solution is 70% thiophanate methyl, 800 times of solution comprehensively.
Embodiment 12
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (4) acclimatization and transplants, step (3) gained micrografting seedling is proceeded to greenhouse initial stage hardening 8 days, proceed to hardening 4-5 week in seedbed again, within 7 days, carry out disinfection to seedbed in advance, the matrix in seedbed is volume ratio is the perlite of 1:2 and the mixture of humus, in described seedbed during hardening, carry out spraying process and sterilization processing, can transplant after hardening terminates, the method for wherein said spraying process is: during fine day, at interval of 15min spraying 3s; When rainy day or cloudy day, at interval of 25min spraying 4s, the time of spraying process is 8 days, and described bactericidal treatments is: used microbicide solution to spray grafting every 8 days, described microbicide solution is 70% thiophanate methyl, 800 times of solution comprehensively.
Embodiment 13
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (4) acclimatization and transplants, step (3) gained micrografting seedling is proceeded to greenhouse initial stage hardening 5 days, proceed to hardening 4-5 week in seedbed again, within 7 days, carry out disinfection to seedbed in advance, the matrix in seedbed is volume ratio is the perlite of 2:3 and the mixture of humus, in described seedbed during hardening, carry out spraying process and sterilization processing, can transplant after hardening terminates, the method for wherein said spraying process is: during fine day, at interval of 15min spraying 3s; When rainy day or cloudy day, at interval of 18min spraying 4s, the time of spraying process is 12 days, and described bactericidal treatments is: used microbicide solution to spray grafting every 5 days, described microbicide solution is 70% thiophanate methyl, 800 times of solution comprehensively.
Embodiment 14
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (4) acclimatization and transplants, step (3) gained micrografting seedling is proceeded to greenhouse initial stage hardening 7 days, proceed to hardening 4-5 week in seedbed again, within 7 days, carry out disinfection to seedbed in advance, the matrix in seedbed is volume ratio is the perlite of 2:3 and the mixture of humus, in described seedbed during hardening, carry out spraying process and sterilization processing, can transplant after hardening terminates, the method for wherein said spraying process is: during fine day, at interval of 10min spraying 3s; When rainy day or cloudy day, at interval of 20min spraying 3s, the time of spraying process is 10 days, and described bactericidal treatments is: used microbicide solution to spray grafting every 7 days, described microbicide solution is 50% carbendazim, 800 times of solution comprehensively.
Embodiment 15
The plantlet in vitro micro-grafting method of the dream of the Japanese maple orange described in the present embodiment, is with the difference of embodiment 1:
In step (4) acclimatization and transplants, step (3) gained micrografting seedling is proceeded to greenhouse initial stage hardening 7 days, proceed to hardening 4-5 week in seedbed again, within 7 days, carry out disinfection to seedbed in advance, the matrix in seedbed is volume ratio is the perlite of 2:3 and the mixture of humus, in described seedbed during hardening, carry out spraying process and sterilization processing, can transplant after hardening terminates, the method for wherein said spraying process is: during fine day, at interval of 10min spraying 3s; When rainy day or cloudy day, at interval of 20min spraying 3s, the time of spraying process is 6 days, and described bactericidal treatments is: used microbicide solution to spray grafting every 7 days, described microbicide solution is 75% tpn, 800 times of solution comprehensively.
Embodiment 16
Respectively grafting wound callus growth time of the grafting of Statistics Implementation example 1-15 group training micro-grafting method, grafting wound healing live time, survival rate, can grafting time, growing way, the results are shown in Table 1.
Table 1 embodiment 1-15 group training micro-grafting method result statistics
Note: in table, "+" represents grafting growing way, it is better that "+" represents growing way more.
As can be seen from the above table, the method of the plantlet in vitro micrografting of embodiment 1-15, its grafting wound healing is fast, and survival rate is higher, growing way is also better, improve the quality of grafting, and the plantlet in vitro micro-grafting method of the application all can carry out in the whole year, do not limit by environment and season, can greatly extend by grafting time, thus the output of grafting is also improved, plantlet in vitro incubation time is short, micrografting seedling incubation time is short, substantially increase the efficiency of breeding, overcome traditional grafting method by season and environmental limitations, grafting time is shorter, operating technology requires high, grafting efficiency is low, the defect that reproductive number is few.As can be seen from embodiment 1-10, in plantlet in vitro micrografting step, medium consist of auxin, sucrose, agar combination condition time, its wound healing is faster, and in MS minimal medium, supplement 1.0mg/L6-BA, 0.05mg/LNAA, 20g/L sucrose, during 5g/L agar, its grafting wound healing time and wound callus growth time are the shortest, are optimal medium condition.Comparative example 1-15, can find out, the grafting wound healing time of the grafting that the technical scheme of embodiment 1 obtains and wound callus growth time are faster, and survival rate is the highest, and growing way is better, is the preferred scheme of the application.Compared to traditional grafting method, technical scheme can be used for micro-potted landscape, micro-potted plant by the less grafting of production specification, and the training of solution group is taken root difficult problem, and its reproduction speed is faster than tradition, little by seasonal effect.
Below be only the preferred embodiment of the present invention, it should be pointed out that above-mentioned preferred embodiment should not be considered as limitation of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For those skilled in the art, without departing from the spirit and scope of the present invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. a plantlet in vitro micro-grafting method for the dream of Japanese maple orange, is characterized in that: said method comprising the steps of:
(1) aseptic stock is cultivated: cut Fructus Aceris elegantulis band bud branch section, carry out tissue cultures, obtain Fructus Aceris elegantulis aseptic seedling, Fructus Aceris elegantulis aseptic seedling cut sth. askew and block, obtain aseptic stock;
(2) aseptic scion cultivated: the dream band bud branch section cutting Japanese maple orange, carries out tissue cultures, obtains the dream aseptic seedling of Japanese maple orange, the dream aseptic seedling of Japanese maple orange cut sth. askew and block, obtain aseptic scion;
(3) plantlet in vitro micrografting: is alignd in the oblique section of the oblique section of the aseptic stock described in step (1) with the described aseptic scion of step (2), and it is fixing, obtain micrografting seedling, described grafting is inoculated on medium and cultivated for 4-5 weeks, consisting of of described medium: MS minimal medium, supplements 0.25-1.50mg/L6-BA, 0.02-0.10mg/LNAA, 5-30g/L sucrose;
(4) acclimatization and transplants: step (3) gained micrografting seedling is proceeded to greenhouse initial stage hardening 5-8 days, then proceed to hardening 4-5 week in seedbed, in described seedbed during hardening, carry out spraying process and sterilization processing, can transplant after hardening terminates.
2. the plantlet in vitro micro-grafting method of the dream of a kind of Japanese maple orange according to claim 1, it is characterized in that: in described plantlet in vitro micrografting, the condition that micrografting seedling is cultivated in the medium is: temperature is 23-27 DEG C, and intensity of illumination is 2300-2600Lx, and light application time is 16h/d.
3. the plantlet in vitro micro-grafting method of the dream of a kind of Japanese maple orange according to claim 1, it is characterized in that: in described plantlet in vitro micrografting, consisting of of described medium: MS minimal medium, supplements 0.5-1.25mg/L6-BA, 0.03-0.07mg/LNAA, 10-25g/L sucrose.
4. the plantlet in vitro micro-grafting method of the dream of a kind of Japanese maple orange according to claim 3, it is characterized in that: in described plantlet in vitro micrografting, consisting of of described medium: MS minimal medium, supplements 1.0mg/L6-BA, 0.05mg/LNAA, 20g/L sucrose.
5. the plantlet in vitro micro-grafting method of the dream of a kind of Japanese maple orange according to claim 1, is characterized in that: in described plantlet in vitro micrografting, and also supplement 3-7g/L agar in described medium, the pH value of described agar is 6.5.
6. the plantlet in vitro micro-grafting method of the dream of a kind of Japanese maple orange according to claim 1, is characterized in that: the stem of described aseptic stock is slightly more than 1.5mm, and the stem of described aseptic scion is slightly more than 1mm.
7. the plantlet in vitro micro-grafting method of the dream of a kind of Japanese maple orange according to claim 1, is characterized in that: in described acclimatization and transplants, and the matrix in described seedbed is volume ratio is 2:(2-4) perlite and the mixture of humus.
8. the plantlet in vitro micro-grafting method of the dream of a kind of Japanese maple orange according to claim 1, is characterized in that: in described acclimatization and transplants, and the method for described spraying process is: during fine day, at interval of 10-15min spraying, 3-4s; When rainy day or cloudy day, at interval of 18-25min spraying, 3-4s, the time of spraying process is 6-12 days.
9. the plantlet in vitro micro-grafting method of the dream of a kind of Japanese maple orange according to claim 1, it is characterized in that: in described acclimatization and transplants, described bactericidal treatments is: used microbicide solution to spray grafting every 5-8 days comprehensively.
10. the plantlet in vitro micro-grafting method of the dream of a kind of Japanese maple orange according to claim 9, is characterized in that: described microbicide solution is selected from 70% thiophanate methyl, 800 times of solution, 50% carbendazim, 800 times of solution, 75% tpn, 800 times of solution for any one.
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| CN108848990A (en) * | 2018-07-17 | 2018-11-23 | 北华大学 | A kind of method that micro-graft cultivates monoecism Chinese yew |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718883A (en) * | 2016-11-28 | 2017-05-31 | 华中农业大学 | A kind of Yunnan Chinese catalpa and the miniature engrafting method of test tube seedling of Chinese catalpa |
| CN108848990A (en) * | 2018-07-17 | 2018-11-23 | 北华大学 | A kind of method that micro-graft cultivates monoecism Chinese yew |
| CN108848990B (en) * | 2018-07-17 | 2020-10-09 | 北华大学 | Method for cultivating hermaphrodite taxus chinensis by micro-grafting |
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