CN103749158A - Micro-grafting method for acer palmatum and acer paimatum under tissue culture condition - Google Patents
Micro-grafting method for acer palmatum and acer paimatum under tissue culture condition Download PDFInfo
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- CN103749158A CN103749158A CN201410006369.3A CN201410006369A CN103749158A CN 103749158 A CN103749158 A CN 103749158A CN 201410006369 A CN201410006369 A CN 201410006369A CN 103749158 A CN103749158 A CN 103749158A
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Abstract
The invention discloses a micro-grafting method for acer palmatum and acer paimatum under a tissue culture condition. The method includes sowing acer palmatum seeds in greenhouses to culture the acer palmatum; selecting acer palmatum which grows healthily as rootstock; manufacturing explants by acer paimatum branches; performing induction culture on auxiliary buds and exercising seedlings; cutting the exercised acer paimatum auxiliary buds to be used as scions and performing micro-grafting on the scions; culturing, exercising and transplanting micro-grafted seedlings to obtain micro-grafted acer paimatum seedlings which grow healthily. The method has the advantages that operation is simple, large quantities of acer paimatum micro-grafted seedlings can be acquired in a short period of time without constraints of environmental factors such as the seasons, the temperatures and the climate, regenerated plants cultured by the method are stable in genetic character and short in nursery period, are healthy and grow vigorously, and the survival rate of the regenerated plants can reach 85% at least.
Description
Technical field
The invention belongs to plant micro-grafting method technical field, be specifically related to your micro-grafting method of Acer palmatum gold under a kind of condition of tissue culture.
Background technology
Aceraceae (
aceraceae) maple genus (
acer) the common general term maple of plant, custom claims again " maple " or " maple ".Whole world maple has kind more than 200, is distributed in Asia, Europe, North America and Africa northern.China has more than 160 to plant, and accounts for the more than 75% of whole world sum, and all there is distribution all parts of the country, the main product Yangtze river basin and on the south each provinces and regions.Maple can be used as construction timber, paper making raw material, medicine extraction, iundustrial oil and nectariferous plant.Tree-like abundant, the attitude of some maple graceful, leaf abundant, leaf look changeable, is described as world-renowned ornamental foliage plant.
Acer palmatum (Acer paimatum) is called refined maple, is that Aceraceae maple belongs to, and is ornamental tree species famous and precious in urban look.Originate in Yangtze River in China basin, also there is distribution in Japan, the Korea peninsula.China is mainly distributed in each province, the Yangtze river basin, is born in the border or sparse woods on the following mountain region of height above sea level 1200m, hills more.Shank Acer defoliation small arbor, high about 8m, bark Dark grey, annual shoot purple or pale purple green; Perennial branch light gray, purple or darkviolet.Acer palmatum happiness is warm, humid climate and half cloudy environment, and intolerant to waterlogging is more drought-enduring, in the isolated planting of direct sunlight place, subjects to a day bright evil summer; Fit and be born in soil fertile, moistening and that draining is good, cold resistance is not strong, acidity, neutrality and calcareous soil all can adapt to, because it has high ornamental value, the performance of urban look is had to considerable effect, the places such as Middle Isolated Grassland of Highway, park, square, greenbelt in little residential region its graceful figure that is seen everywhere is the good ornamental tree species of competitively cultivating in recent years.Countries in the world are introducing and planting already, and mutation and modification are a lot.Japan's Acer palmatum is cultivated more than 450 reward leaf kinds at present.
Jin Gui (Acer palmatum ' katsura ') has another name called: " card Soviet Union " is that the variation of Acer palmatum gardens one of is planted.Spring young leaves glassy yellow, edge is orange red, summer, blade became light greenly to yellowish green, autumn, leaf color became again bright orange to orange.Blade is mostly 5 and splits, and splits deeply, and there is shallow tooth at edge.Its vertical growth, plant type is compact.Jin Gui is a very attracting maple kind, but it is very difficult for breeding, and cuttage root-taking rate is low, and tissue culture propagation technical system is not also set up at present, and seed germination rate is low, and production quantity does not far reach the market demand.
Micrografting refers under condition of tissue culture the technology that stock and scion are carried out to grafting, if can set up your micrografting system of Acer palmatum gold under condition of tissue culture, can reach the object of carrying out throughout the year the expensive seedling of the fast numerous gold of short-term.By By consulting literatures, aspect the golden expensive group training of Acer palmatum micrografting, have not been reported.
Summary of the invention
object of the present invention is to overcome the deficiencies in the prior art, provide the Acer palmatum gold under a kind of condition of tissue culture your micro-grafting method, the method is simple to operate, can within short-term, obtain the expensive grafting of a large amount of gold, and be not subject to the restriction of season, temperature, weather, the expensive plant stabilization characteristics of genetics of gold that the method is cultivated, plant be healthy, it is vigorous to grow, growing-seedling period is short, survival rate is high.
The object of the invention is to be achieved through the following technical solutions: your micro-grafting method of Acer palmatum gold under a kind of condition of tissue culture, it comprises the following steps:
S1. obtaining of stock: sow Acer palmatum seed and cultivate in booth seedbed, when Acer palmatum plant strain growth to 30～40cm height, choose the plant of robust growth as stock;
S2. obtaining of scion: gather the expensive branch of gold that diameter is 2.5～3.5mm in fine day, being cut into length is the explant that 3.5～4.5cm trains for group with the stem section of 2 buds, explant is seeded on inducing culture and is cultivated, until golden your explant bud, moved into booth hardening 3～4d during in half germinating, the axillalry bud cutting after hardening is scion, while noting cutting, scion needs band portion xylem, and scion length is 0.8～1.2cm; Wherein, described inducing culture is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L; Under the bud of described explant bud, position retains the stem that length is 4.5～5.5mm;
S3. micrografting: the stock that step S1 is obtained is blocking apart from root 4.5～5.5cm place, with micrografting cutter, at stock truncation surface place, vertically split out the otch that the degree of depth is 1.2～1.8cm, by the formation layer alignment stock of step S2 gained scion, in insertion otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, be placed in canopy and cultivate; Keeping humidity in canopy is >=90%, and temperature is 23～28 ℃, cultivates 18～22d;
S5. acclimatization and transplants: the micrografting seedling that above-mentioned cultivation is survived moves in another canopy, and slowly reduce humidity and shift out golden your micrografting seedling to outer humidity.
The invention has the beneficial effects as follows:
1. the present invention utilizes propagation by grafiting know-why, by methods such as your axillalry bud of tissue culture and inducement gold, Multiple Buds, within compared with short-term, obtained the expensive scion of a large amount of gold and carried out micrografting, obtained a large amount of Jin Guimiao, set up golden your group training micrografting propagation technique system, for the expensive breeding of gold provides a new way.
2. utilize the inventive method graft seedling growth can obtain whole plant in 20～30 days, really realize high efficiency quick breeding.The regeneration plant stabilization characteristics of genetics that the method is cultivated, plant be healthy, it is vigorous to grow, growing-seedling period is short, survival rate can reach more than 85%.
3. because the obtaining of the obtaining of the inventive method stock, scion, micrografting, cultivation and acclimatization and transplants are to realize, be not therefore subject to the restriction of the envirment factors such as season, weather and temperature under artificial control.
Accompanying drawing explanation
Fig. 1 is for cultivating the expensive micrografting seedling of the gold schematic diagram of 15 days;
Fig. 2 is for cultivating the expensive micrografting seedling of the gold schematic diagram of 20 days;
Fig. 3 is for cultivating the expensive micrografting seedling of the gold schematic diagram of 30 days;
Fig. 4 is the expensive micrografting seedling of the gold after acclimatization and transplants schematic diagram.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, and protection scope of the present invention is not limited to the following stated.
embodiment 1:your micro-grafting method of Acer palmatum gold under condition of tissue culture, it comprises the following steps:
S1. obtaining of stock: sow Acer palmatum seed and cultivate in booth seedbed, during to 30cm height, choose the plant of robust growth as stock until Acer palmatum plant strain growth;
S2. obtaining of scion: gather the expensive branch of gold that diameter is 2.5mm in fine day, being cut into length is the explant that 3.5cm trains for group with the stem section of 2 buds, explant is seeded on inducing culture and is cultivated, until golden your explant bud, moved into booth hardening 3d during in half germinating, the axillalry bud cutting after hardening is scion, while noting cutting, scion needs band portion xylem, and scion length is 0.8cm; Wherein, described inducing culture is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L; Under the bud of described explant bud, position retains the stem that length is 4.5mm;
S3. micrografting: the stock that step S1 is obtained is blocking apart from root 4.5cm place, with micrografting cutter, at stock truncation surface place, vertically split out the otch that the degree of depth is 1.2cm, by the formation layer alignment stock of step S2 gained scion, in insertion otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, be placed in canopy and cultivate, as shown in Figure 1, Figure 2, Figure 3 shows; Keeping humidity in canopy is 90%, and temperature is 23 ℃, cultivates 18d;
S5. acclimatization and transplants: the micrografting seedling that above-mentioned cultivation is survived moves in another canopy, and slowly reduce humidity in canopy and shift out golden your micrografting seedling to extraneous humidity, as shown in Figure 4.
embodiment 2:your micro-grafting method of Acer palmatum gold under condition of tissue culture, it comprises the following steps:
S1. obtaining of stock: sow Acer palmatum seed and cultivate in booth seedbed, during to 40cm height, choose the plant of robust growth as stock until Acer palmatum plant strain growth;
S2. obtaining of scion: gather the expensive branch of gold that diameter is 3.5mm in fine day, being cut into length is the explant that 4.5cm trains for group with the stem section of 2 buds, explant is seeded on inducing culture and is cultivated, until golden your explant bud, moved into booth hardening 4d during in half germinating, under gnotobasis, the axillalry bud cutting after hardening is scion, and while noting cutting, scion needs band portion xylem, and scion length is 1.2cm; Wherein, described inducing culture is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L; Under the bud of described explant bud, position retains the stem that length is 5.5mm;
S3. micrografting: the stock that step S1 is obtained is blocking apart from root 5.5cm place, with micrografting cutter, at stock truncation surface place, vertically split out the otch that the degree of depth is 1.8cm, by the formation layer alignment stock of step S2 gained scion, in insertion otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, be placed in canopy and cultivate, as shown in Figure 1, Figure 2, Figure 3 shows; Keeping humidity in canopy is 92%, and temperature is 28 ℃, cultivates 22d;
S5. acclimatization and transplants: the micrografting seedling that above-mentioned cultivation is survived moves in another canopy, and slowly reduce humidity in canopy and transplant golden your micrografting seedling to extraneous humidity, as shown in Figure 4.
embodiment 3:your micro-grafting method of Acer palmatum gold under condition of tissue culture, it comprises the following steps:
S1. obtaining of stock: sow Acer palmatum seed and cultivate in booth seedbed, during to 35cm height, choose the plant of robust growth as stock until Acer palmatum plant strain growth;
S2. obtaining of scion: gather the expensive branch of gold that diameter is 3mm in fine day, being cut into length is the explant that 4cm trains for group with the stem section of 2 buds, explant is seeded on inducing culture and is cultivated, until golden your explant bud, moved into booth hardening 3.5d during in half germinating, the axillalry bud cutting after hardening is scion, while noting cutting, scion needs band portion xylem, and scion length is 1cm; Wherein, described inducing culture is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L; Under the bud of described explant bud, position retains the stem that length is 5mm;
S3. micrografting: the stock that step S1 is obtained is blocking apart from root 5cm place, with micrografting cutter, at stock truncation surface place, vertically split out the otch that the degree of depth is 1.5cm, the formation layer alignment stock by the scion of step S2 gained, inserts in otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, be placed in canopy and cultivate, as shown in Figure 1, Figure 2, Figure 3 shows; Keeping humidity in canopy is 95%, and temperature is 25 ℃, cultivates 18～22d;
S5. acclimatization and transplants: the micrografting seedling that above-mentioned cultivation is survived moves in another canopy, and slowly reduce humidity in canopy and shift out golden your micrografting seedling to extraneous humidity, as shown in Figure 4.
embodiment 4:your micro-grafting method of Acer palmatum gold under condition of tissue culture, it comprises the following steps:
S1. obtaining of stock: sow Acer palmatum seed and cultivate in booth seedbed, during to 38cm height, choose the plant of robust growth as stock until Acer palmatum plant strain growth;
S2. obtaining of scion: gather the expensive branch of gold that diameter is 3.5mm in fine day, being cut into length is the explant that 3.5cm trains for group with the stem section of 2 buds, explant is seeded on inducing culture and is cultivated, until golden your explant bud, moved into booth hardening 3d during in half germinating, under gnotobasis, the axillalry bud cutting after hardening is scion, and while noting cutting, scion needs band portion xylem, and scion length is 0.9cm; Wherein, described inducing culture is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L; Under the bud of described explant bud, position retains the stem that length is 4.8mm;
S3. micrografting: the stock that step S1 is obtained is blocking apart from root 5.2cm place, with micrografting cutter, at stock truncation surface place, vertically split out the otch that the degree of depth is 1.7cm, by the formation layer alignment stock of step S2 gained scion, in insertion otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, be placed in canopy and cultivate, as shown in Figure 1, Figure 2, Figure 3 shows; Keeping humidity in canopy is 93%, and temperature is 26 ℃, cultivates 22d;
S5. acclimatization and transplants: the micrografting seedling that above-mentioned cultivation is survived moves in another canopy, and slowly reduce temperature of shed and humidity is transplanted golden your micrografting seedling to ambient temperature and humidity, as shown in Figure 4.
Claims (3)
1. your micro-grafting method of the gold of the Acer palmatum under condition of tissue culture, is characterized in that, it comprises the following steps:
S1. obtaining of stock: sow Acer palmatum seed and cultivate in booth seedbed, when Acer palmatum plant strain growth to 30～40cm height, choose the plant of robust growth as stock;
S2. obtaining of scion: gather the expensive branch of gold that diameter is 2.5～3.5mm in fine day, being cut into length is the explant that 3.5～4.5cm trains for group with the stem section of 2 buds, explant is seeded on inducing culture and is cultivated, until golden your explant bud, moved into booth hardening 3～4d during in half germinating, the axillalry bud cutting after hardening is scion, while noting cutting, scion needs band portion xylem, and scion length is 0.8～1.2cm;
S3. micrografting: the stock that step S1 is obtained is blocking apart from root 4.5～5.5cm place, with micrografting cutter, at stock truncation surface place, vertically split out the otch that the degree of depth is 1.2～1.8cm, by the formation layer alignment stock of step S2 gained scion, in insertion otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, be placed in canopy and cultivate; Keeping humidity in canopy is >=90%, and temperature is 23～28 ℃, cultivates 18～22d;
S5. acclimatization and transplants: the micrografting seedling that above-mentioned cultivation is survived moves in another canopy, and slowly reduce humidity in canopy and shift out golden your micrografting seedling to extraneous humidity.
2. your micro-grafting method of the gold of the Acer palmatum under a kind of condition of tissue culture according to claim 1, is characterized in that, inducing culture described in step S2 is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L.
3. your micro-grafting method of the gold of the Acer palmatum under a kind of condition of tissue culture according to claim 1, is characterized in that, under the bud of the bud of explant described in step S2, position retains the stem that length is 4.5～5.5mm.
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Cited By (3)
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| CN104429635A (en) * | 2014-11-17 | 2015-03-25 | 苏州市新巷农艺科技园 | Grafting propagation method for albo-purpurascens |
| CN105519434A (en) * | 2015-12-30 | 2016-04-27 | 四川禾木本业农林科技有限公司 | A tissue cultured seedling micrografting method for acer palmatum 'orange dream' |
| CN106417025A (en) * | 2016-10-14 | 2017-02-22 | 山西农业大学 | Test tube micro-grafting method for seedless grape embryo-rescued malformed plantlets |
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| CN106417025A (en) * | 2016-10-14 | 2017-02-22 | 山西农业大学 | Test tube micro-grafting method for seedless grape embryo-rescued malformed plantlets |
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Address after: 610041 B 12F, Air China Century Center, No. 1, air route, Sichuan, Chengdu, Wuhou District Patentee after: VICFARM MANUFACTURE ASSOCIATION Address before: 610041 B 12F, Air China Century Center, No. 1, air route, Sichuan, Chengdu, Wuhou District Patentee before: VIMA MANUFACTURE ASSOCIATION |
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Effective date of registration: 20190621 Address after: 635600 Tissue Culture Workshop Building of Rare and Colorful Leaf Plant Varieties in Guangwu Mountain, Sanshe, Changtan Village, Zhengzheng Town, Nanjiang County, Bazhong City, Sichuan Province Patentee after: Sichuan Qicai Forestry Co., Ltd. Address before: 610041 Block 12F, Air Century Center B, No. 1 Aviation Road, Wuhou District, Chengdu City, Sichuan Province Patentee before: VICFARM MANUFACTURE ASSOCIATION |