CN103749158B - Micro-grafting method for acer palmatum and acer paimatum under tissue culture condition - Google Patents
Micro-grafting method for acer palmatum and acer paimatum under tissue culture condition Download PDFInfo
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- CN103749158B CN103749158B CN201410006369.3A CN201410006369A CN103749158B CN 103749158 B CN103749158 B CN 103749158B CN 201410006369 A CN201410006369 A CN 201410006369A CN 103749158 B CN103749158 B CN 103749158B
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Abstract
The invention discloses a micro-grafting method for acer palmatum and acer paimatum under a tissue culture condition. The method includes sowing acer palmatum seeds in greenhouses to culture the acer palmatum; selecting acer palmatum which grows healthily as rootstock; manufacturing explants by acer paimatum branches; performing induction culture on auxiliary buds and exercising seedlings; cutting the exercised acer paimatum auxiliary buds to be used as scions and performing micro-grafting on the scions; culturing, exercising and transplanting micro-grafted seedlings to obtain micro-grafted acer paimatum seedlings which grow healthily. The method has the advantages that operation is simple, large quantities of acer paimatum micro-grafted seedlings can be acquired in a short period of time without constraints of environmental factors such as the seasons, the temperatures and the climate, regenerated plants cultured by the method are stable in genetic character and short in nursery period, are healthy and grow vigorously, and the survival rate of the regenerated plants can reach 85% at least.
Description
Technical field
The invention belongs to plant micro-grafting method technical field, be specifically related to your micro-grafting method of Acer palmatum gold under a kind of condition of tissue culture.
Background technology
Aceraceae (
aceraceae) Acer L (
acer) the usual general term maple of plant, be accustomed to also known as " maple " or " maple ".Whole world maple has kind more than 200, is distributed in Asia, Europe, North America and Africa northern.China has more than 160 to plant, and account for more than 75% of whole world sum, all there is distribution all parts of the country, the main product Yangtze river basin and on the south each provinces and regions.Maple can be used as construction timber, paper making raw material, medicine extraction, iundustrial oil and nectariferous plant.Tree-like abundant, the attitude of some maple graceful, leaf abundant, leaf look changeable, is described as world-renowned ornamental foliage plant.
Acer palmatum (Acer paimatum) calls refined maple, is Aceraceae Acer L, is ornamental tree species famous and precious in urban look.Originate in Yangtze River in China basin, also there is distribution in Japan, the Korea peninsula.China is mainly distributed in Yangtze river basin each province, is born in below height above sea level 1200m mountain region, the border on hills or sparse woods more.Shank Acer defoliation small arbor, height is 8m about, bark Dark grey, annual shoot purple or pale purple green; Perennial branch light gray, purple or darkviolet.Acer palmatum warm, the humid climate of happiness and half cloudy environment, intolerant to waterlogging, more drought-enduring, to subject to day bright evil summer in the isolated planting of direct sunlight place; Suitable be born in fertile, moistening and draining is good soil, cold resistance is not strong, acid, neutral and calcareous soil all can adapt to, because it has high ornamental value, to the performance of urban look, there is considerable effect, the figure of local its grace that is seen everywhere such as Middle Isolated Grassland of Highway, park, square, greenbelt in little residential region is the excellent ornamental tree species competitively cultivated in recent years.Countries in the world are introducing and planting already, mutation and modification a lot.Japan's Acer palmatum cultivates more than 450 reward leaf kinds at present.
Jin Gui (Acer palmatum ' katsura ') has another name called: " card Soviet Union ", is that the variation of Acer palmatum gardens one of is planted.Spring young leaves glassy yellow, edge is orange red, and summer, blade became light green to yellowish green, and autumn, leaf color became again bright orange to orange.Blade is mostly 5 and splits, and split dark, there is shallow tooth at edge.Its vertical growth, plant type is compact.Jin Gui is a very attracting maple kind, but it is very not easily bred, and cutting plantation is low, and tissue culture propagation technical system is not also set up at present, and seed germination rate is low, and production quantity does not far reach the market demand.
Micrografting refers to the technology of stock and scion being carried out grafting under condition of tissue culture, if can set up your micrografting system of Acer palmatum gold under condition of tissue culture, can reach the object of carrying out the expensive seedling of the fast numerous gold of short-term throughout the year.By By consulting literatures, have not been reported in the group training micrografting that Acer palmatum gold is expensive.
Summary of the invention
namely object of the present invention is to overcome the deficiencies in the prior art, there is provided the Acer palmatum gold under a kind of condition of tissue culture your micro-grafting method, the method is simple to operate, the expensive grafting of a large amount of gold can be obtained within short-term, and not by the restriction of season, temperature, weather, gold expensive plant stabilization characteristics of genetics, plant that the method is cultivated be healthy, it is vigorous to grow, growing-seedling period is short, survival rate is high.
The object of the invention is to be achieved through the following technical solutions: your micro-grafting method of Acer palmatum gold under a kind of condition of tissue culture, it comprises the following steps:
S1. the acquisition of stock: sow Acer palmatum seed and cultivate in booth seedbed, when Acer palmatum plant strain growth to 30 ~ 40cm height, chooses the plant of robust growth as stock;
S2. the acquisition of scion: gather the expensive branch of gold that diameter is 2.5 ~ 3.5mm in fine day, to be cut into length be the stem section of 3.5 ~ 4.5cm with 2 buds is the explant of group training, explant is seeded on inducing culture and cultivates, booth hardening 3 ~ 4d is moved into when your explant bud golden is in half germinating, cutting the axillalry bud after hardening is scion, when noting cutting, scion needs band portion xylem, and scion length is 0.8 ~ 1.2cm; Wherein, described inducing culture is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L; It is the stem of 4.5 ~ 5.5mm that the bud lower portion of described explant bud retains length;
S3. micrografting: the stock obtained by step S1 blocks at distance root 4.5 ~ 5.5cm place, vertically split out at stock truncation surface place the otch that the degree of depth is 1.2 ~ 1.8cm with micrografting cutter, by the formation layer of step S2 gained scion alignment stock, in insertion otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, is placed in canopy and cultivates; Keep canopy humidity to be >=90%, temperature is 23 ~ 28 DEG C, cultivates 18 ~ 22d;
S5. acclimatization and transplants: the micrografting seedling above-mentioned cultivation survived moves in another canopy, and shift out your micrografting seedling golden after slowly reducing humidity to outer humidity.
The invention has the beneficial effects as follows:
1. the present invention utilizes propagation by grafiting know-why, by your method such as axillalry bud, Multiple Buds of tissue culture and inducement gold, within comparatively short-term, obtain a large amount of your scion golden and carry out micrografting, obtain a large amount of Jin Guimiao, establish Jin Gui group training micrografting propagation technique system, for the breeding that gold is expensive provides a new way.
2. utilize the inventive method graft seedling growth can obtain whole plant in 20 ~ 30 days, really realize high efficiency quick breeding.Regeneration plant stabilization characteristics of genetics, plant that the method is cultivated be healthy, it is vigorous to grow, growing-seedling period is short, survival rate can reach more than 85%.
3. because the acquisition of the acquisition of the inventive method stock, scion, micrografting, cultivation and acclimatization and transplants realize under artificial control, therefore not by the restriction of the envirment factors such as season, weather and temperature.
Accompanying drawing explanation
Fig. 1 is the cultivation gold of 15 days expensive micrografting seedling schematic diagram;
Fig. 2 is the cultivation gold of 20 days expensive micrografting seedling schematic diagram;
Fig. 3 is the cultivation gold of 30 days expensive micrografting seedling schematic diagram;
Fig. 4 is the gold expensive micrografting seedling schematic diagram after acclimatization and transplants.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, and protection scope of the present invention is not limited to the following stated.
embodiment 1:your micro-grafting method of Acer palmatum gold under condition of tissue culture, it comprises the following steps:
S1. the acquisition of stock: sow Acer palmatum seed and cultivate in booth seedbed, when Acer palmatum plant strain growth is to 30cm height, chooses the plant of robust growth as stock;
S2. the acquisition of scion: gather the expensive branch of gold that diameter is 2.5mm in fine day, to be cut into length be the stem section of 3.5cm with 2 buds is the explant of group training, explant is seeded on inducing culture and cultivates, booth hardening 3d is moved into when your explant bud golden is in half germinating, cutting the axillalry bud after hardening is scion, when noting cutting, scion needs band portion xylem, and scion length is 0.8cm; Wherein, described inducing culture is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L; It is the stem of 4.5mm that the bud lower portion of described explant bud retains length;
S3. micrografting: the stock obtained by step S1 blocks at distance root 4.5cm place, vertically split out at stock truncation surface place the otch that the degree of depth is 1.2cm with micrografting cutter, by the formation layer of step S2 gained scion alignment stock, in insertion otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, is placed in canopy and cultivates, as shown in Figure 1, Figure 2, Figure 3 shows; Keep canopy humidity to be 90%, temperature is 23 DEG C, cultivates 18d;
S5. acclimatization and transplants: the micrografting seedling above-mentioned cultivation survived moves in another canopy, and shift out your micrografting seedling golden after slowly reducing canopy humidity to ambient humidity, as shown in Figure 4.
embodiment 2:your micro-grafting method of Acer palmatum gold under condition of tissue culture, it comprises the following steps:
S1. the acquisition of stock: sow Acer palmatum seed and cultivate in booth seedbed, when Acer palmatum plant strain growth is to 40cm height, chooses the plant of robust growth as stock;
S2. the acquisition of scion: gather the expensive branch of gold that diameter is 3.5mm in fine day, to be cut into length be the stem section of 4.5cm with 2 buds is the explant of group training, explant is seeded on inducing culture and cultivates, booth hardening 4d is moved into when your explant bud golden is in half germinating, in an aseptic environment, cutting the axillalry bud after hardening is scion, and when noting cutting, scion needs band portion xylem, and scion length is 1.2cm; Wherein, described inducing culture is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L; It is the stem of 5.5mm that the bud lower portion of described explant bud retains length;
S3. micrografting: the stock obtained by step S1 blocks at distance root 5.5cm place, vertically split out at stock truncation surface place the otch that the degree of depth is 1.8cm with micrografting cutter, by the formation layer of step S2 gained scion alignment stock, in insertion otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, is placed in canopy and cultivates, as shown in Figure 1, Figure 2, Figure 3 shows; Keep canopy humidity to be 92%, temperature is 28 DEG C, cultivates 22d;
S5. acclimatization and transplants: the micrografting seedling above-mentioned cultivation survived moves in another canopy, and transplant your micrografting seedling golden after slowly reducing canopy humidity to ambient humidity, as shown in Figure 4.
embodiment 3:your micro-grafting method of Acer palmatum gold under condition of tissue culture, it comprises the following steps:
S1. the acquisition of stock: sow Acer palmatum seed and cultivate in booth seedbed, when Acer palmatum plant strain growth is to 35cm height, chooses the plant of robust growth as stock;
S2. the acquisition of scion: gather the expensive branch of gold that diameter is 3mm in fine day, to be cut into length be the stem section of 4cm with 2 buds is the explant of group training, explant is seeded on inducing culture and cultivates, booth hardening 3.5d is moved into when your explant bud golden is in half germinating, cutting the axillalry bud after hardening is scion, when noting cutting, scion needs band portion xylem, and scion length is 1cm; Wherein, described inducing culture is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L; It is the stem of 5mm that the bud lower portion of described explant bud retains length;
S3. micrografting: the stock obtained by step S1 blocks at distance root 5cm place, vertically split out at stock truncation surface place the otch that the degree of depth is 1.5cm with micrografting cutter, by the formation layer of step S2 gained scion alignment stock, insert in otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, is placed in canopy and cultivates, as shown in Figure 1, Figure 2, Figure 3 shows; Keep canopy humidity to be 95%, temperature is 25 DEG C, cultivates 18 ~ 22d;
S5. acclimatization and transplants: the micrografting seedling above-mentioned cultivation survived moves in another canopy, and shift out your micrografting seedling golden after slowly reducing canopy humidity to ambient humidity, as shown in Figure 4.
embodiment 4:your micro-grafting method of Acer palmatum gold under condition of tissue culture, it comprises the following steps:
S1. the acquisition of stock: sow Acer palmatum seed and cultivate in booth seedbed, when Acer palmatum plant strain growth is to 38cm height, chooses the plant of robust growth as stock;
S2. the acquisition of scion: gather the expensive branch of gold that diameter is 3.5mm in fine day, to be cut into length be the stem section of 3.5cm with 2 buds is the explant of group training, explant is seeded on inducing culture and cultivates, booth hardening 3d is moved into when your explant bud golden is in half germinating, in an aseptic environment, cutting the axillalry bud after hardening is scion, and when noting cutting, scion needs band portion xylem, and scion length is 0.9cm; Wherein, described inducing culture is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L; It is the stem of 4.8mm that the bud lower portion of described explant bud retains length;
S3. micrografting: the stock obtained by step S1 blocks at distance root 5.2cm place, vertically split out at stock truncation surface place the otch that the degree of depth is 1.7cm with micrografting cutter, by the formation layer of step S2 gained scion alignment stock, in insertion otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, is placed in canopy and cultivates, as shown in Figure 1, Figure 2, Figure 3 shows; Keep canopy humidity to be 93%, temperature is 26 DEG C, cultivates 22d;
S5. acclimatization and transplants: the micrografting seedling above-mentioned cultivation survived moves in another canopy, and transplant your micrografting seedling golden after slowly reducing temperature of shed and humidity to ambient temperature and humidity, as shown in Figure 4.
Claims (2)
1. your micro-grafting method of Acer palmatum gold under condition of tissue culture, it is characterized in that, it comprises the following steps:
S1. the acquisition of stock: sow Acer palmatum seed and cultivate in booth seedbed, when Acer palmatum plant strain growth to 30 ~ 40cm height, chooses the plant of robust growth as stock;
S2. the acquisition of scion: gather the expensive branch of gold that diameter is 2.5 ~ 3.5mm in fine day, to be cut into length be the stem section of 3.5 ~ 4.5cm with 2 buds is the explant of group training, explant is seeded on inducing culture and cultivates, booth hardening 3 ~ 4d is moved into when your explant bud golden is in half germinating, cutting the axillalry bud after hardening is scion, when noting cutting, scion needs band portion xylem, and scion length is 0.8 ~ 1.2cm; Described inducing culture is MS+6-BA 0.5mg/L+IAA 0.8mg/L+ sucrose 20g/L+ agar 6g/L;
S3. micrografting: the stock obtained by step S1 blocks at distance root 4.5 ~ 5.5cm place, vertically split out at stock truncation surface place the otch that the degree of depth is 1.2 ~ 1.8cm with micrografting cutter, by the formation layer of step S2 gained scion alignment stock, in insertion otch, with plastic seal membrana oralis bale packing interface, obtain micrografting seedling;
S4. cultivate: micrografting seedling is planted in basin, is placed in canopy and cultivates; Keep canopy humidity to be >=90%, temperature is 23 ~ 28 DEG C, cultivates 18 ~ 22d;
S5. acclimatization and transplants: the micrografting seedling above-mentioned cultivation survived moves in another canopy, and shift out your micrografting seedling golden after slowly reducing canopy humidity to ambient humidity.
2. your micro-grafting method of Acer palmatum gold under a kind of condition of tissue culture according to claim 1, is characterized in that, in step S2, the bud lower portion of explant bud retains length is the stem of 4.5 ~ 5.5mm.
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| CN104429635A (en) * | 2014-11-17 | 2015-03-25 | 苏州市新巷农艺科技园 | Grafting propagation method for albo-purpurascens |
| CN105519434B (en) * | 2015-12-30 | 2017-11-28 | 四川七彩林业开发有限公司 | A kind of tissue-cultured seedling micro-grafting method of Japanese maple orange dream |
| CN106417025A (en) * | 2016-10-14 | 2017-02-22 | 山西农业大学 | Test tube micro-grafting method for seedless grape embryo-rescued malformed plantlets |
| CN117814034A (en) * | 2024-01-31 | 2024-04-05 | 中国热带农业科学院香料饮料研究所 | Seedling raising method for bread fruits |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2080780C1 (en) * | 1994-05-11 | 1997-06-10 | Всероссийский научно-исследовательский институт сельскохозяйственной биотехнологии РАСХН | Clonal plant micro propagation method |
| CN102792857A (en) * | 2012-08-16 | 2012-11-28 | 江苏省林业科学研究院 | Acer palmatum twig grafting propagation method |
| CN103004593A (en) * | 2012-12-11 | 2013-04-03 | 浙江农林大学 | Thin-shell pecan tissue culture bud micro grafting method |
| CN103053346A (en) * | 2013-01-25 | 2013-04-24 | 河南省木本饲料工程技术研究中心 | Micrografting method for tetraploid acacia |
| CN103477983A (en) * | 2013-09-06 | 2014-01-01 | 巴中市光雾山植物研究所 | Acer palmatum Sangokaku tissue culture propagation process |
-
2014
- 2014-01-07 CN CN201410006369.3A patent/CN103749158B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2080780C1 (en) * | 1994-05-11 | 1997-06-10 | Всероссийский научно-исследовательский институт сельскохозяйственной биотехнологии РАСХН | Clonal plant micro propagation method |
| CN102792857A (en) * | 2012-08-16 | 2012-11-28 | 江苏省林业科学研究院 | Acer palmatum twig grafting propagation method |
| CN103004593A (en) * | 2012-12-11 | 2013-04-03 | 浙江农林大学 | Thin-shell pecan tissue culture bud micro grafting method |
| CN103053346A (en) * | 2013-01-25 | 2013-04-24 | 河南省木本饲料工程技术研究中心 | Micrografting method for tetraploid acacia |
| CN103477983A (en) * | 2013-09-06 | 2014-01-01 | 巴中市光雾山植物研究所 | Acer palmatum Sangokaku tissue culture propagation process |
Non-Patent Citations (3)
| Title |
|---|
| 《植物微嫁接研究进展》;周艳等;《贵州科学》;20130415(第2期);第84-88页 * |
| 《美国红枫组织培养与快繁技术的研究》;曹受金等;《湖北农业科学》;20101105(第11期);第2643-2645页 * |
| 巴中市林业局.《七彩林业,槭树研究硕果累累》.《http://www.bzsly.gov.cn/show.asp?id=4270》.2013,第7段. * |
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Address after: 610041 B 12F, Air China Century Center, No. 1, air route, Sichuan, Chengdu, Wuhou District Patentee after: VICFARM MANUFACTURE ASSOCIATION Address before: 610041 B 12F, Air China Century Center, No. 1, air route, Sichuan, Chengdu, Wuhou District Patentee before: VIMA MANUFACTURE ASSOCIATION |
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Effective date of registration: 20190621 Address after: 635600 Tissue Culture Workshop Building of Rare and Colorful Leaf Plant Varieties in Guangwu Mountain, Sanshe, Changtan Village, Zhengzheng Town, Nanjiang County, Bazhong City, Sichuan Province Patentee after: Sichuan Qicai Forestry Co., Ltd. Address before: 610041 Block 12F, Air Century Center B, No. 1 Aviation Road, Wuhou District, Chengdu City, Sichuan Province Patentee before: VICFARM MANUFACTURE ASSOCIATION |