CN103053346A - Micrografting method for tetraploid acacia - Google Patents
Micrografting method for tetraploid acacia Download PDFInfo
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- CN103053346A CN103053346A CN201310030244XA CN201310030244A CN103053346A CN 103053346 A CN103053346 A CN 103053346A CN 201310030244X A CN201310030244X A CN 201310030244XA CN 201310030244 A CN201310030244 A CN 201310030244A CN 103053346 A CN103053346 A CN 103053346A
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Abstract
The invention relates to a micrografting method for a tetraploid acacia. The method includes the steps of selecting a seedling of a diploid acacia seed as a rootstock, selecting a tissue culture seedling of the tetraploid acacia as a scion, subjecting the scion and the rootstock to cambium alignment by using a cleft grafting method, twining the interface area with silver paper slips, fixing the silver paper twining area with a grafting clamp, planting the grafted seedling into a potted tray containing matrix, then processing a routine management for 20 - 30 days, and acquiring the plant regeneration. By means of the micrografting method, an integral plant is obtained after cultivating the grafted seedling for 20 - 30 days, and accordingly the high efficiency of rapid planting is achieved. The plant regeneration cultivated by the micrografting method has the advantages of stable genetic character, healthy plant, strong growth, short seedling period, fast plant growing and high survival rate ( with a survival rate over 90% in a field planting production land).
Description
Technical field
The invention belongs to plant micro-grafting method technical field, be specifically related to a kind of micro-grafting method that is applicable to tetraploid locust.
Background technology
Extensive plantation along with tetraploid locust, its fast-growing that has, drought resisting, barren-resistant, Salt And Alkali Tolerance, fertility are strong etc., and good characteristic is not fully exerted in China's forestry ecological benefit, having obtained good ecological benefits, economic benefit and social benefit, is the first-selected fine tree species such as forests for windbreak and sand-fixation, field safeguarding forest, green barren hill and conceding the land to forestry.The development and utilization of tetraploid locust is played positive facilitation to the development of forest industry.Yet the propagation method of tetraploid locust is mostly because there being the defectives such as processing step is numerous and diverse, survival rate is low, growing-seedling period is long, so that restricted applying of tetraploid locust at present.
Summary of the invention
The object of the invention is to provide a kind of tetraploid locust micro-grafting method that is applicable to, and the plant strain growth that the method is cultivated is vigorous, and growing-seedling period is short, and field planting production land for growing field crops survival rate is high.
For achieving the above object, the present invention adopts following technical scheme:
A kind of tetraploid locust micro-grafting method, the method selects dliploid Robinia pseudoacacia Seeds seedling to make stock, tetraploid locust group training seedling is done scion, adopt cleft graft that scion and stock are formed layer alignment, twine interface with the tinfoil bar, fixedly tinfoil winding place of Grafting clip is planted grafting in the cave dish that matrix is housed and then to be carried out Routine Management, obtains regeneration plant after 20-30 days.
Concrete, described dliploid Robinia pseudoacacia Seeds seedling as stock obtains through following preliminary treatment: selection full grains, disease-free dliploid Robinia pseudoacacia Seeds, after 70-75 ℃ water soaking 3-5 minute, take out, then be seeded in vernalization on the seedbed of fine sand with behind 20-30 ℃ emerge in worm water 1-2 days, keep 25-28 ℃ of seedling bed temperature, humidity 75-90% to be beneficial to seed sprouting, seed sprouting after 3-7 days when treating that seedling grows to true leaf and is about to launch, namely can be used as stock.
Described matrix is preferably turfy soil and the perlite of weight 1:1.Matrix in the dish of cave is in advance with the aqueous solution disinfection that contains 0.2% potassium permanganate and 0.01% carbendazim.Matrix adopts turfy soil and perlite to mix and uses, and humidity is agglomerating to hold, fall into ground distributor after unclamping scatters and be advisable.
Described Routine Management is specially: grafting plant water immediately in the cave dish that matrix is housed once permeable, then change in the booth with automatically control spraying management, one all spraying concentration 0.01-0.03%(percentage by weights) carbendazol (morbidity of avoiding infection) and the MS liquid nutrient medium (without sucrose) of dilution after 100 times each 2-3 time, wound healing is finished after 10-15 days, remove Grafting clip and tinfoil after 20-30 days, obtain regeneration plant.
Carry out in the following manner when spraying the MS liquid nutrient medium: after the macroelement mother liquor in the medium and micro-mother liquor are respectively diluted 100 times, sooner or later alternately spray, week spray gets final product for 2-3 time.
Compare with existing method, micro-grafting method of the present invention has following advantage:
1) adopt the inventive method to need not to consider the plant resources shortage.Utilize tetraploid locust group training seedling and dliploid Robinia pseudoacacia Seeds seedling to carry out grafting, solved the plant resources shortage phenomenon that causes because of scion or stock deficiency fully, widen the new varieties of knowing clearly and breed approach, utilizability is stronger, seedling grafting survival rate high (graft survival rate is more than 70%).
2) utilize the inventive method grafting to cultivate and to obtain whole plant in 20-30 days, really realize high efficiency quick breeding.The regeneration plant stabilization characteristics of genetics that this method is cultivated, plant is healthy, and it is vigorous to grow, and growing-seedling period is short, and seedling is fast, motility rate high (field planting production land for growing field crops survival rate is more than 90%).The tetraploid locust of grafting becomes to have higher feeding value and economic worth, is beneficial to its Multiplication and extension.
3) be not subjected to the restriction in season.The maximum characteristics of the inventive method are that numerous seedling cycle is fast, and traditional grafting is harsh to the temperature requirement in grafting season, and season limit is stronger.And the micrografting technology adopts the equipment control of full exposure auto spraying, and is not enough such as illumination, and light-supplementing system is arranged; But the excess Temperature automatic cooling provides good growing environment to grafting, improves survival rate, and is not subject to seasonal restrictions, and Zhou Nianjun can realize that grafting breeds.In addition, because the method adopts the automatic management pattern aspect water spray, fertilising, irrigation, temperature control, the convenient operation management is beneficial to the input that reduces labour and management cost, saves production cost.
4) the present invention adopts loose matrix (perlite and turfy soil, fine sand) to cultivate and hardening, can not exert an influence to root system during transplanting, is beneficial to improve the survival rate of transplanting seedlings.
Embodiment
Below by specific embodiment technical scheme of the present invention is described, but protection scope of the present invention is not limited to this.
Embodiment 1
A kind of tetraploid locust micro-grafting method, the method comprises the steps:
1) obtains dliploid Robinia pseudoacacia Seeds seedling: select full grains, disease-free dliploid Robinia pseudoacacia Seeds, take out after 5 minutes with 70 ℃ water soakings, then be seeded in after 2 days with vernalization on the seedbed of fine sand with 20 ℃ emerge in worm water, keep 25 ℃ of seedling bed temperatures, humidity 80-85% to be beneficial to seed sprouting, seed sprouting after 7 days, when treating that seedling grows to true leaf and is about to launch, namely get the seedling that can be used as stock.
2) make stock with dliploid Robinia pseudoacacia Seeds seedling, tetraploid locust group training seedling is done scion, adopt cleft graft (to be specially: dliploid Robinia pseudoacacia Seeds seedling is taken out from husky bed, from terminal bud below 1cm revolving knife, in that incision vertical cutting 1.5cm is for subsequent use again, the selection plant development is normal, about height 3cm, rugosity and rootstock seedling adapt tetraploid locust group training seedling do scion, its base portion is cut into about the long 1.0cm of wedge surface, adopt cleft graft that the scion of handling well is inserted in the ready stock longitudinal incision) scion and stock are formed layer alignment, twine interface with the tinfoil bar, fixedly tinfoil winding place of Grafting clip is planted grafting in the cave dish that matrix (described matrix is mixed by turfy soil and the perlite of weight 1:1) is housed and then to be carried out Routine Management.
3) Routine Management: grafting is planted and waters immediately once permeablely in the cave dish that matrix is housed, and then changes (the control environment that keeps 25 ℃ of temperature, humidity 70-80% in the booth in the booth with automatically control spraying management over to.Humidity is excessively low, easily cause the plant dehydration here to wither and cause death), all spraying concentration 0.01%(percentage by weights) carbendazol and the MS liquid nutrient medium after 100 times of the dilutions each 3 times, wound healings are finished after two weeks, remove Grafting clip and tinfoil after 20 days, obtain regeneration plant.
Embodiment 2
A kind of tetraploid locust micro-grafting method, the method comprises the steps:
1) obtains dliploid Robinia pseudoacacia Seeds seedling: select full grains, disease-free dliploid Robinia pseudoacacia Seeds, take out after 3 minutes with 75 ℃ water soakings, then be seeded in after 1 day with vernalization on the seedbed of fine sand with 28 ℃ emerge in worm water, keep 27 ℃ of seedling bed temperatures, humidity 80-85% to be beneficial to seed sprouting, seed sprouting after 5 days, when treating that seedling grows to true leaf and is about to launch, namely get the seedling that can be used as stock.
2) make stock with dliploid Robinia pseudoacacia Seeds seedling, tetraploid locust group training seedling is done scion, adopt cleft graft (to be specially: dliploid Robinia pseudoacacia Seeds seedling is taken out from husky bed, from terminal bud below 1cm revolving knife, in that incision vertical cutting 1.5cm is for subsequent use again, the selection plant development is normal, about height 3cm, rugosity and rootstock seedling adapt tetraploid locust group training seedling do scion, its base portion is cut into about the long 1.0cm of wedge surface, adopt cleft graft that the scion of handling well is inserted in the ready stock longitudinal incision) scion and stock are formed layer alignment, twine interface with the tinfoil bar, fixedly tinfoil winding place of Grafting clip is planted grafting in the cave dish that matrix (described matrix is mixed by turfy soil and the perlite of weight 1:1) is housed and then to be carried out Routine Management.
3) Routine Management: grafting plant water immediately in the cave dish that matrix is housed once permeable, then change over to (control environment that keeps 25 ℃ of temperature, humidity 70-80% in the booth) in the booth with automatically control spraying management, one all spraying concentration 0.03%(percentage by weights) carbendazol and the MS liquid nutrient medium of dilution after 100 times each 2 times, wound healing is finished after 12 days, remove Grafting clip and tinfoil after 28 days, obtain regeneration plant.
Claims (4)
1. tetraploid locust micro-grafting method, it is characterized in that, the method selects dliploid Robinia pseudoacacia Seeds seedling to make stock, tetraploid locust group training seedling is done scion, adopt cleft graft that scion and stock are formed layer alignment, twine interface, fixedly tinfoil winding place of Grafting clip with the tinfoil bar, grafting planted in the cave dish that matrix is housed then carry out Routine Management, obtain regeneration plant after 20-30 days.
2. tetraploid locust micro-grafting method as claimed in claim 1, it is characterized in that, described dliploid Robinia pseudoacacia Seeds seedling as stock obtains through following preliminary treatment: selection full grains, disease-free dliploid Robinia pseudoacacia Seeds, after 70-75 ℃ water soaking 3-5 minute, take out, then be seeded in vernalization on the seedbed of fine sand with behind 20-30 ℃ emerge in worm water 1-2 days, keep 25-28 ℃ of seedling bed temperature, humidity 75-90%, seed sprouting after 3-7 days, when treating that seedling grows to true leaf and is about to launch, namely can be used as stock.
3. tetraploid locust micro-grafting method as claimed in claim 1 is characterized in that, described matrix is turfy soil and the perlite of weight 1:1.
4. tetraploid locust micro-grafting method as claimed in claim 1, it is characterized in that, described Routine Management is specially: grafting plant water immediately in the cave dish that matrix is housed once permeable, then change in the booth with automatically control spraying management, MS liquid nutrient medium after spraying the carbendazol of weight percent concentration 0.01-0.03% one week and diluting 100 times each 2-3 time, wound healing is finished after 10-15 days, removes Grafting clip and tinfoil after 20-30 days, obtains regeneration plant.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103392407A (en) * | 2013-07-30 | 2013-11-20 | 山西五洲昕宇农林科技发展有限公司 | Method for promoting germination and sowing by quickly treating Chinese scholartree seeds |
| CN103749158A (en) * | 2014-01-07 | 2014-04-30 | 四川胜泽源农业投资有限公司 | Micro-grafting method for acer palmatum and acer paimatum under tissue culture condition |
| CN104012305A (en) * | 2014-06-18 | 2014-09-03 | 永州市力群木业有限公司 | Locust tree breeding and grafting method for improving quality and fruiting rate |
| CN105052665A (en) * | 2015-09-15 | 2015-11-18 | 管天球 | Method for cultivating improved varieties of paraphlomis javanica var. coronata (Vaniot) big seedlings |
| CN105900702A (en) * | 2016-04-25 | 2016-08-31 | 广西大学 | Method for improving fertility of tetraploid cucumis melo |
| CN106613363A (en) * | 2016-09-26 | 2017-05-10 | 山东省林业科学研究院 | Cultural method for umbrella-type locust transplanted to street tree |
| CN106718883A (en) * | 2016-11-28 | 2017-05-31 | 华中农业大学 | A kind of Yunnan Chinese catalpa and the miniature engrafting method of test tube seedling of Chinese catalpa |
| CN108834604A (en) * | 2018-08-20 | 2018-11-20 | 河北北方学院 | The method of potato tetraploid cultivar and wild diploid species distant hybridization |
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| CN101530050A (en) * | 2009-03-12 | 2009-09-16 | 新疆大学 | Thin-skin walnut indoor micro-branch engrafting method |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392407A (en) * | 2013-07-30 | 2013-11-20 | 山西五洲昕宇农林科技发展有限公司 | Method for promoting germination and sowing by quickly treating Chinese scholartree seeds |
| CN103749158A (en) * | 2014-01-07 | 2014-04-30 | 四川胜泽源农业投资有限公司 | Micro-grafting method for acer palmatum and acer paimatum under tissue culture condition |
| CN103749158B (en) * | 2014-01-07 | 2015-07-01 | 四川胜泽源农业投资有限公司 | Micro-grafting method for acer palmatum and acer paimatum under tissue culture condition |
| CN104012305A (en) * | 2014-06-18 | 2014-09-03 | 永州市力群木业有限公司 | Locust tree breeding and grafting method for improving quality and fruiting rate |
| CN105052665A (en) * | 2015-09-15 | 2015-11-18 | 管天球 | Method for cultivating improved varieties of paraphlomis javanica var. coronata (Vaniot) big seedlings |
| CN105900702A (en) * | 2016-04-25 | 2016-08-31 | 广西大学 | Method for improving fertility of tetraploid cucumis melo |
| CN105900702B (en) * | 2016-04-25 | 2017-04-26 | 广西大学 | Method for improving fertility of tetraploid cucumis melo |
| CN106613363A (en) * | 2016-09-26 | 2017-05-10 | 山东省林业科学研究院 | Cultural method for umbrella-type locust transplanted to street tree |
| CN106718883A (en) * | 2016-11-28 | 2017-05-31 | 华中农业大学 | A kind of Yunnan Chinese catalpa and the miniature engrafting method of test tube seedling of Chinese catalpa |
| CN108834604A (en) * | 2018-08-20 | 2018-11-20 | 河北北方学院 | The method of potato tetraploid cultivar and wild diploid species distant hybridization |
| CN108834604B (en) * | 2018-08-20 | 2021-01-15 | 河北北方学院 | Distant hybridization method for potato tetraploid cultivars and diploid wild species |
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Application publication date: 20130424 |