CN111869362B - Method for improving seed coat permeability and seed embryo respiration of hard seed - Google Patents
Method for improving seed coat permeability and seed embryo respiration of hard seed Download PDFInfo
- Publication number
- CN111869362B CN111869362B CN202010763278.XA CN202010763278A CN111869362B CN 111869362 B CN111869362 B CN 111869362B CN 202010763278 A CN202010763278 A CN 202010763278A CN 111869362 B CN111869362 B CN 111869362B
- Authority
- CN
- China
- Prior art keywords
- seed
- soaking
- seeds
- penetrant
- hard
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
Abstract
The invention discloses a method for improving seed coat permeability and seed embryo respiration of hard seed, belonging to the technical field of seedling breeding. Alternatively treating seeds at 50 deg.C or above with hot water containing penetrant and at 0 deg.C or below by quenching to make seed coat have more and larger capillaries or pores, soaking seeds in oxygen-releasing agent, and soaking in CO2The absorbent soaking liquid is soaked alternately, and the soaking liquid contains penetrant to facilitate the solution to penetrate the seed coat to facilitate absorption and expansion. The method has low cost, is easy to operate and is suitable for popularization.
Description
Technical Field
The present invention belongs to the field of seedling breeding technology. In particular to a method for increasing permeability of seed coats and promoting oxygen and carbon dioxide exchange of seeds to release dormancy of hard seeds.
Background
Seeds which are difficult to be subjected to imbibition germination due to the hard seed coat (sometimes including the pericarp) are called hard seed, and hard seeds are widely present in plant seeds and are commonly found in leguminosae, liliaceae, malvaceae, Convolvulaceae, Theaceae, Palmaceae and the like. The degree of firmness is related to the hierarchy and arrangement of the seed coat cells, the type and content of pigments, and the deposition of impermeable substances (including cutin, colloid, wax, suberin, hemicellulose, etc.) (the poplar stage, et al. mechanism and method of elimination of dormancy of hard seed. botany report, 2006,23(1): 108-118). "the seed coat of hard seed often limits the gas exchange of the embryo and hinders O2And CO2Thereby inhibiting respiration and not ensuring the energy required for germination. Generally, the water permeability of most seeds with poor air permeability is also weak, but some hard seeds of plants have strong water permeability and can absorb water, but the air permeability is weak; even though these seeds have absorbed sufficient water, due to O2Failure to inhale, possible CO2And the gas cannot be discharged, so that the gas exchange is not smooth, and the biochemical reaction cannot be smoothly carried out (Yang Shi and, et al. mechanism and method for dormancy of hard seeds. botany report, 2006,23(1): 108-118). Some hard seed also accompany physiological dormancy of embryos, so that dormancy of hard seed is often the result of interaction of multiple factors. The hard material is treated by mechanical damage, low temperature and high temperature treatment, wet and dry treatment, radiation, high pressure treatment, etc. and chemical treatment, such as acid etching, alkali soaking, organic solvent, etc.
The cutin, wax, colloid and other hydrophobic substances of the seed coat form a low-energy surface, and the surface energy of water is large, the surface tension is higher than the critical surface tension of the seed coat, so that the seed coat is not easy to be soaked and permeated by water, and the wetting agent mainly reduces the solid-liquid interfacial tension and increases the wetting and contact of the liquid medicine on the surface of a treatment object (harmful organisms, plants and the like). The penetrant increases the penetration of the liquid medicine to the surface of the treated object and promotes the absorption of the liquid medicine (Zhang zong. application and research progress of pesticide adjuvant. pesticide science and management, 2009, 30 (1): 42-47). Osmosis refers to the phenomenon that liquid enters capillary tubes or porous solids, and osmotic agents have good wetting effect, but only a few types of wetting agents have good osmotic effect (Zhonghong. Manchu wetting and osmosis. Jiangsu silk, 1995, (2) 20-51. Wei Du, et al. discussion of pesticide-wetting osmotic agents. surfactant industry, 1994(1):19-24,14), such as nekal and osmotic agent JFC.
The hard seed coat is made of hydrophobic substances, and the seed coat does not have enough capillaries or pores, so that water is not easy to permeate into the seed coat and is absorbed by the embryo. Therefore, in order to allow the solution to penetrate the seed coat and be absorbed by the embryo, it is necessary to satisfy the two conditions of the seed coat having sufficiently large and numerous pores and the solution surface tension being reduced by the penetrating agent.
Disclosure of Invention
The invention mainly aims to provide a method for improving the permeability of seed coats and the seed embryo respiration of hard seed seeds, so that the seed coats have more capillaries or pores, the solution is easy to permeate the seed coats and be absorbed by the seed embryos, the gas exchange of the seed embryos is enhanced, the dormancy relieving is promoted, and the method is beneficial to the imbibition and the germination of seeds.
A method for improving the permeability of seed coat and the respiration of seed embryo of hard seed includes such steps as immersing the seed in hot water containing penetrant, which is favorable to the penetration of hot water into seed coat via capillary or pore space, dissolving part of wax and ester, taking out the seed, draining, low-temp freezing, and freezing.
Another problem to be solved by the present invention is that the seed soaking solution contains oxygen releasing agent, CO2The absorbent is added with the penetrating agent, the solution penetrates the seed coat, oxygen is provided for the seed embryo and carbon dioxide released by the seed is absorbed, smooth respiration of the seed embryo is ensured, and metabolism is promoted. The hydrogen peroxide solution can be slowly decomposed into water and oxygen at normal temperature, is a common oxygen release agent, stimulates a pentose phosphate pathway of endosperm substance metabolism by hydrogen peroxide with a certain concentration, can also participate in signal marks of hormones, has a synergistic effect with gibberellin, and has an important effect on relieving seed dormancy (Marangong, and the like, the hydrogen peroxide oxygenation causes the influence on the activity of tobacco seeds. Chinese tobacco science 2015, 36 (5): 8-12) and can be used as a seed germination promoter. The application of alcohol amine solution for absorbing CO currently2The main trapping method has the advantages of fast absorption, large absorption capacity, low cost, desorption and regeneration of alcohol amine, etc. in addition, the alcohol amine solution is alkaline, and the alkali liquor soaking is one of the first-choice methods for softening the wax on the seed skin, so that the moisture can easily permeate the seed coat to be absorbed by the seed embryo.
The technical scheme provided by the invention is as follows:
a method for improving the permeability of seed coat and the respiration of embryo of hard seed includes adding penetrant in hot water at 50 deg.C or higher, immersing the seeds while stirring for 10-30 min, taking out the seeds, draining water, freezing at 0 deg.C or lower for 3-30 min, immersing in hot water containing penetrant, freezing at 0 deg.C or lower, and repeating said steps for 2-8 times. Soaking seeds in oxygen releasing agent seed soaking liquid containing penetrant and CO containing penetrant2The absorbent soaking liquid is alternately soaked, and one soaking liquid is replaced every 12 or 24 hours until the seeds are sucked and swelled.
The oxygen releasing agent is hydrogen peroxide, and the concentration of the oxygen releasing agent in the seed soaking liquid is 0.05-0.5%.
Said CO2The absorbent is alcohol amine solution, ethanolamine or ethanolamine and N-methyldiethanolamine are selected to be mixed in equal volume, and the concentration of the alcohol amine in the seed soaking liquid0.5～5％。
The penetrant is stable in hard water and acid and alkali resistant, one of diisobutyl naphthalene sulfonate and polyoxyethylene lauryl ether or the mixture of the diisobutyl naphthalene sulfonate and the polyoxyethylene lauryl ether in the volume ratio of 2-8:1 is selected, and the concentration of the penetrant in the seed soaking solution is 0.03-0.3%.
The invention has the beneficial effects that: the hard seed is repeatedly frozen in hot water containing penetrant, and the seed coat is torn more and has larger pores, thus creating conditions for seed soaking liquid penetration. The penetrating agent is used as oxygen releasing agent and CO for soaking liquid2The absorbent is easy to permeate seed coat and seed embryo to absorb, promotes respiratory metabolism, and the oxygen-releasing agent hydrogen peroxide has certain physiological effect of relieving dormancy, and CO2The absorbent alcohol amine also has the functions of softening seed coats and facilitating water penetration. The method has low cost, is easy to operate and is suitable for popularization.
Detailed Description
Example one
1. Method of producing a composite material
Canna indica (Canna indica) seeds have a hard seed rate of more than 90 percent, and can germinate by scratching seed coats or soaking the seeds in concentrated sulfuric acid. Seeds are soaked in tap water for 48 hours, and the seeds with hard and non-imbibition peel are selected and dried for testing. The seeds are divided into 4 groups, the processing method is shown in the table, the temperature-changing processing refers to that the seeds are soaked in 80 ℃ hot water added with penetrant for 15 minutes, the penetrant is mixed solution of sodium diisobutylnaphthalenesulfonate and polyoxyethylene lauryl ether (the volume ratio is 4:1), the concentration is 0.2 percent of the seed soaking solution, the seeds are stirred during the period, then the seeds are fished out, the water is drained, the seeds are immediately transferred into a freezing box for freezing for 20 minutes at (-20 ℃), then the seeds are immediately transferred into 80 ℃ hot water containing the penetrant for soaking, and the quick heating and quick cooling are repeated for 5 times. "active ingredient of seed soaking liquid" refers to seed soaking liquid containing 0.1% hydrogen peroxide oxygen releasing agent and 2% ethanolamine CO2The soaking liquid of the absorbent is added with mixed liquid (volume ratio is 4:1) of sodium diisobutylnaphthalenesulfonate and polyoxyethylene lauryl ether, the concentration is 0.2% of the soaking liquid, the seeds are alternately soaked in the two soaking liquids, and one seed is replaced every 24 hours.
After the seeds are soaked for 4 days, the seeds are obviously enlarged, and the imbibition rate (the number of imbibition seeds/the number of tested seeds) multiplied by 100 percent is counted when the volume is twice of the original volume. And 4 groups of the imbibition seeds germinate in a dark incubator at the temperature of 25 ℃, wherein the germination standard is that the length of the protruded seed coat of the radicle is half of the length of the seeds, the germination vigor is counted on the 6 th day, and the germination rate is counted on the 24 th day.
2. Results
The results of the four seed treatments are shown in the table.
3. Conclusion
The seed soaking liquid contains active component and penetrant, and has no temperature varying treatment, low seed sucking rate and very low germination rate.
Secondly, the seeds are subjected to temperature change treatment, and the imbibition rate is obviously improved compared with that of the first group. The repeated shock heating and quenching (and penetrating agent) effect of the seeds is demonstrated, so that the permeability of the seed coat is increased, and the water absorption is facilitated.
The temperature-changing treatment of the seeds and the synergistic effect of the penetrating agent of the seed soaking liquid are achieved, the absorption rate is obviously improved compared with that of the seeds in the group II, the use of the penetrating agent is proved, and the water absorption capacity of the seeds is further improved.
The temperature-changing treatment, the synergistic effect of the active components of the seed soaking liquid and the penetrating agent and the further improved absorption-expansion rate compared with the third group are performed on the seed soaking liquid, and the alkaline alcohol amine contained in the seed soaking liquid is favorable for softening seed coat cutin, absorbing and expanding seeds, releasing oxygen agent and CO2The absorbent can promote metabolism (hydrolysis of nutrient components in seeds) and relieve dormancy of water swelling. The germination potential of the group IV is higher than that of the group III, which shows that the active ingredient (hydrogen peroxide) of the soak solution of the group IV enhances the vitality of the seeds and leads the seeds to germinate faster.
Example two
1. Method of producing a composite material
The seed hard seed rate of Rhus chinensis (Rhus chinensis) is about 90%. Soaking seeds in tap water for 24h, selecting seeds with hard and non-imbibition peelThe seeds were dried for testing. The seeds are divided into 4 groups, the processing method is shown in the table, the 'temperature changing processing' means that the seeds are soaked in hot water at 70 ℃ for 20 minutes, the penetrant is sodium diisobutylnaphthalenesulfonate, the concentration is 0.2%, the seeds are stirred in the period, the seeds are taken out, the water is drained, the seeds are put into liquid nitrogen (-196 ℃) for freezing for 5 minutes, the seeds are immediately soaked in the hot water at 70 ℃ containing the penetrant, and the quick heating and the quick cooling are repeated for 3 times. "active ingredient of seed soaking liquid" refers to seed soaking liquid containing 0.1% hydrogen peroxide as oxygen releasing agent and 2% ethanolamine in CO2The two seed soaking liquids of the absorbent are both added with sodium diisobutylnaphthalenesulfonate serving as a penetrant, the concentration of the sodium diisobutylnaphthalenesulfonate is 0.2 percent, the seeds are alternately soaked in the two seed soaking liquids, and one seed soaking liquid is replaced every 12 hours.
After the seeds are soaked for 2 days, the seeds are obviously enlarged, and the seeds with the volume being twice of the original volume are taken as the imbibition rate (the number of the imbibition seeds/the number of the seeds to be tested) multiplied by 100 percent. The imbibition seeds all germinate in a constant temperature incubator at 25 ℃, the illumination is carried out for 12 hours every day, the germination standard is that the length of the radicle protruding seed coat is half of the length of the seeds, the germination vigor is counted on the 5 th day, and the germination rate is counted on the 12 th day.
2. Results
The results of the four seed treatments are shown in the table.
3. Conclusion
Firstly, the seeds in the seed group are not subjected to temperature change treatment, the seed soaking liquid contains active ingredients and does not contain a penetrating agent, and the seed imbibition rate and germination are low.
And secondly, the temperature change treatment of the seeds is performed, the imbibition rate and the germination rate are obviously improved compared with the first step, the repeated sudden heating and quenching temperature change (and penetrating agent) effect of the seeds is demonstrated, the permeability of the seed coat is increased, and the water absorption is facilitated.
The temperature-changing treatment of the seeds and the synergistic effect of the penetrating agent of the seed soaking liquid improve the absorption rate compared with the seeds in the group II, which shows that the penetrating agent is used to further improve the water absorption capacity of the seeds.
The temperature change treatment, the synergistic effect of the active ingredients and the penetrating agent and the obvious increase of the imbibition rate compared with the third group indicate that the group of seed soaking liquid contains alkaline alcoholThe amine is effective in softening cutin of seed coat, promoting seed swelling, and releasing oxygen and CO2The absorbent can promote metabolism (hydrolysis of nutritional seed components) and relieve dormancy. The germination vigor of the group IV is higher than that of the group III, which shows that the active ingredient (hydrogen peroxide) of the soak solution of the group IV enhances the seed vitality and leads the seeds to germinate faster.
Claims (2)
1. A method for improving seed coat permeability and seed embryo respiration of hard seed is characterized in that: the hard seed is canna seed, the seed is soaked in 80 deg.C hot water containing penetrant for 15 min, the seed is fished out and frozen at-20 deg.C for 20 min, the soaking and freezing are repeated for 5 times, and the seed is soaked in oxygen releasing agent containing penetrant and CO containing penetrant2Alternately soaking absorbent seed soaking solutions, replacing one seed soaking solution every 24 hours, soaking for 4 days, and sucking and swelling seeds;
the penetrant is sodium diisobutylnaphthalenesulfonate and polyoxyethylene lauryl ether which are mixed according to the volume ratio of 4:1, and the concentration of the soaking liquid is 0.2%;
the oxygen release agent is hydrogen peroxide, and the concentration of the oxygen release agent in the seed soaking liquid is 0.1 percent;
said CO2The absorbent is ethanolamine, and the concentration of the absorbent in the seed soaking liquid is 2 percent.
2. A method for improving seed coat permeability and seed embryo respiration of hard seed is characterized in that: the hard seed is Rhus chinensis Mill seed, soaking in 70 deg.C hot water containing penetrant for 20 min, taking out, freezing in liquid nitrogen at-196 deg.C for 5 min, repeating the soaking and freezing for 3 times, soaking in oxygen releasing agent containing penetrant and CO containing penetrant2Alternately soaking the absorbent seed soaking liquid, replacing one seed soaking liquid every 12 hours, soaking for 2 days, and absorbing and swelling the seeds;
the penetrant is sodium diisobutylnaphthalenesulfonate, and the concentration of the penetrant in the seed soaking solution is 0.2%;
the oxygen release agent is hydrogen peroxide, and the concentration of the oxygen release agent in the seed soaking liquid is 0.1 percent;
said CO2The absorbent is ethanolamine, and the concentration of the soaking liquidThe degree was 2%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010763278.XA CN111869362B (en) | 2020-08-01 | 2020-08-01 | Method for improving seed coat permeability and seed embryo respiration of hard seed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010763278.XA CN111869362B (en) | 2020-08-01 | 2020-08-01 | Method for improving seed coat permeability and seed embryo respiration of hard seed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111869362A CN111869362A (en) | 2020-11-03 |
| CN111869362B true CN111869362B (en) | 2022-05-03 |
Family
ID=73205061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010763278.XA Active CN111869362B (en) | 2020-08-01 | 2020-08-01 | Method for improving seed coat permeability and seed embryo respiration of hard seed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111869362B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115362867A (en) * | 2022-07-19 | 2022-11-22 | 中国科学院新疆生态与地理研究所 | Saline water seedling growing method for sophora isojavanica |
| CN115316068B (en) * | 2022-08-12 | 2023-09-05 | 内蒙古天创药业科技有限公司 | Method for treating astragalus seeds |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
| CN103477754A (en) * | 2013-10-11 | 2014-01-01 | 韩庆军 | Sassafras tsumu seed dormancy breaking and germination accelerating method suitable for Shandong region |
| CN104798489A (en) * | 2015-05-12 | 2015-07-29 | 镇江鑫源达园艺科技有限公司 | Method for breaking morning glory seed dormancy |
| CN105684595A (en) * | 2016-01-19 | 2016-06-22 | 河北省林业科学研究院 | Method for breaking dormancy of hybrid seeds of bearded iris |
| CN106900208A (en) * | 2017-04-05 | 2017-06-30 | 安徽咱家田生态农业有限公司 | A kind of germination accelerating method of peppermint seed |
| CN107864713A (en) * | 2017-11-18 | 2018-04-03 | 蚌埠市徽吉星农业科技农民专业合作社 | A kind of processing method for accelerating China tree germination |
| CN107873178A (en) * | 2017-10-28 | 2018-04-06 | 蚌埠宏瑞园林有限公司 | A kind of germination accelerating method for improving canna percentage of seedgermination |
| CN110313267A (en) * | 2019-07-29 | 2019-10-11 | 佛山科学技术学院 | A method of promoting bitter buckwheat seed germination |
-
2020
- 2020-08-01 CN CN202010763278.XA patent/CN111869362B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
| CN103477754A (en) * | 2013-10-11 | 2014-01-01 | 韩庆军 | Sassafras tsumu seed dormancy breaking and germination accelerating method suitable for Shandong region |
| CN104798489A (en) * | 2015-05-12 | 2015-07-29 | 镇江鑫源达园艺科技有限公司 | Method for breaking morning glory seed dormancy |
| CN105684595A (en) * | 2016-01-19 | 2016-06-22 | 河北省林业科学研究院 | Method for breaking dormancy of hybrid seeds of bearded iris |
| CN106900208A (en) * | 2017-04-05 | 2017-06-30 | 安徽咱家田生态农业有限公司 | A kind of germination accelerating method of peppermint seed |
| CN107873178A (en) * | 2017-10-28 | 2018-04-06 | 蚌埠宏瑞园林有限公司 | A kind of germination accelerating method for improving canna percentage of seedgermination |
| CN107864713A (en) * | 2017-11-18 | 2018-04-03 | 蚌埠市徽吉星农业科技农民专业合作社 | A kind of processing method for accelerating China tree germination |
| CN110313267A (en) * | 2019-07-29 | 2019-10-11 | 佛山科学技术学院 | A method of promoting bitter buckwheat seed germination |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111869362A (en) | 2020-11-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111869362B (en) | Method for improving seed coat permeability and seed embryo respiration of hard seed | |
| Gaff | Desiccation-tolerant flowering plants in southern Africa | |
| AU6465599A (en) | A method of performing an impregnating or extracting treatment on a resin-containing wood substrate | |
| CN107414992A (en) | A kind of processing technology of handicraft bamboo chip | |
| CN105684595B (en) | There is the method for whisker iris hybrid seed breaking dormancy | |
| KR102299294B1 (en) | Improved method for seed priming | |
| CN110720411B (en) | Method for removing egg membrane in grouper hatching process | |
| CN103665437A (en) | Preparation method of bacterial cellulose/graphene composite material | |
| Visviki et al. | Growth and cytology of Chlamydomonas acidophila under acidic stress. | |
| CN107471366B (en) | A kind of redwood dehumidification process based on inhibition cell activity | |
| CN107081825A (en) | A kind of method for preventing poplar craze and transfiguration | |
| US5627132A (en) | Method and composition for plant preservation without leaf curling | |
| CN110326390B (en) | Seed treatment method | |
| CN111869370A (en) | Chinese tallow tree seed treatment and coating method | |
| CN108515596B (en) | A kind of preparation method of insect prevention timber | |
| CN107326122A (en) | A kind of leather processing inorganic agent and preparation method thereof | |
| Singh et al. | Morpho-anatomical diversity of roots of Syzygium cumini Skeels (Myrtaceae): an adaptive strategy under stress ecosystem | |
| CN108243657A (en) | The method of Rapid reversal mountain fourth seed dormancy | |
| CN102523783A (en) | Method for treating ginseng seeds by using reagent | |
| Fujisawa | Phases of water absorption in germinating Raphanus embryo | |
| CN111295971B (en) | Method for rapidly breaking dormancy of osmanthus fragrans seeds | |
| CN110001267B (en) | Pearl oyster shell without cuticle edge cracking phenomenon and preparation method thereof | |
| CN111247900A (en) | Method for rapidly releasing dormancy of quercus cyclobalanopsis seeds | |
| TANG et al. | Ecological adaptation mechanisms of roots to flooded soil and respiration characteristics of knee roots of Taxodium Ascendens | |
| JPS6211599A (en) | Sewater desalination method by mangrobe |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220415 Address after: 150000 Guangxian Road, core area of Hanan industrial new town, economic development zone, Harbin, Heilongjiang Applicant after: Heilongjiang canghao Agricultural Development Co.,Ltd. Address before: 401, 154 Huixiang garden, Qixing West Road, Siming District, Xiamen City, Fujian Province Applicant before: Liu Zeyang |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |