CN108243657A - The method of Rapid reversal mountain fourth seed dormancy - Google Patents
The method of Rapid reversal mountain fourth seed dormancy Download PDFInfo
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- CN108243657A CN108243657A CN201810155326.XA CN201810155326A CN108243657A CN 108243657 A CN108243657 A CN 108243657A CN 201810155326 A CN201810155326 A CN 201810155326A CN 108243657 A CN108243657 A CN 108243657A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The present invention provides a kind of methods of Rapid reversal mountain fourth seed dormancy, include the following steps：Mountain fourth fruit is put into the mixed aqueous solution of potassium hydroxide, sodium hydroxide and ethylene glycol and is impregnated, it is then placed in the acetum added with saccharomycete and ferments, high pressure water washing is used again, until pericarp, pulp and seed are kept completely separate, mountain fourth seed is obtained, mountain fourth seed is put into the mixed liquor of pectase and water and is impregnated, is filtered, it blots, mountain fourth seed must be blotted；Mountain fourth seed will be blotted and be put into the mixture of mountain sand, sodium chloride and zeolite and rotate stir-frying, then impregnated with liquor potassic permanganate；Mountain fourth seed is put into Gibberellins solution again and is impregnated；Cold stratification finally is carried out to mountain fourth seed.The present invention can fast and effeciently release the suspend mode of mountain fourth seed, greatly shorten mountain fourth seed and sprout the required time, so as to improve the nursery rate of mountain fourth.
Description
Technical field
The present invention relates to a kind of methods for releasing seed dormancy.It is more particularly related to a kind of Rapid reversal mountain
The method of the sub- seed dormancy of fourth, belongs to field of plant growing technology.
Background technology
Mountain fourth is also known as dwarf apple, be rose family Malus deciduous tree, up to 14m, be mainly distributed on Liaoning, Jilin,
In the mountain forest or shrubbery in the provinces such as Heilungkiang, the Inner Mongol, Hebei, Shanxi, Shandong, Shaanxi, Gansu.Mountain fourth is grey, Ye Lv, branch
Red, three is mutually set off, and is had high ornamental value, is a kind of important Landscape Trees, and containing rich in fruit
Rich vitamin, amino acid and trace element etc., available for making wine, manufacturing the food such as fruit juice, jam；Mountain fourth can be additionally used in
Fruit-tree grafting (such as grafting apple), is a kind of good graft stock.Since mountain fourth has high ornamental value and economy
Value, it is increasing to its demand in the market in recent years, the phenomenon that there is mountain fourth seedling supply falls short of demand.Mountain fourth
Seed there are apparent dormancy, in its natural state breaking dormancy, the time needed be up to 6 months.At present, mountain fourth children
The major way that the cultivation of seedling uses is seminal propagation, and Yin Haibin once delivered a paper about mountain fourth nursery, in paper
Described method for culturing seedlings takes too long, can not meet the needs in market.How the suspend mode of Rapid reversal mountain fourth seed, shorten
Time needed for nursery is still the problem of current urgent need to resolve.
Invention content
It is an object of the present invention to provide a kind of methods of Rapid reversal mountain fourth seed dormancy, can be fast and effeciently
The suspend mode of mountain fourth seed is released, mountain fourth seed is greatly shortened and sprouts the required time.
In order to realize these purposes and other advantages according to the present invention, provide a kind of Rapid reversal mountain fourth seed and stop
The method of dormancy, includes the following steps：
Step 1：By mountain fourth fruit be put into pH be 10, mass ratio 2:2:1 potassium hydroxide, sodium hydroxide and second two
It in the mixed aqueous solution of alcohol, impregnates for 24 hours, takes out at 40 DEG C, rinsed 3 times with pure water, it is molten to be then placed in the acetic acid that pH is 5.0
In liquid, while saccharomycete is added in, ferment 18h at 25 DEG C, takes out, and obtains the mountain fourth fruit of fermentation, then with pressure is 6MPa's
Flow rinses the mountain fourth fruit of fermentation, until pericarp, pulp and seed are kept completely separate, mountain fourth seed is obtained, by mountain fourth kind
It is 1 that son, which is put into mass ratio,:It in 15 pectase and the mixed liquor of water, is stirred at 40 DEG C for 24 hours, filtering was washed out with pure water
The remaining impurity of mountain fourth the surface of the seed after filter, and the pure water of remained on surface is blotted with filter paper, mountain fourth seed must be blotted,
Wherein, pH is first adjusted to 3.5 by the mixed liquor of pectase and water using preceding with hydrochloric acid；
Step 2：The mountain fourth seed that blots that step 1 obtains is added to mass ratio as 2:2:1 mountain sand, sodium chloride and
It in the mixture of zeolite, stirs evenly, is placed in 40 DEG C, rotation stir-frying 1h under conditions of 2000r/min, cooling, separation is come out of retirement and taken up an official post fourth
The mountain fourth seed isolated is placed in the liquor potassic permanganate that mass fraction is 0.1% and impregnates 1h by sub- seed, after taking-up,
It is In Shade to dry with aseptic water washing 3~5 times；
Step 3：By step 2 treated mountain fourth seed is put into a concentration of 100~500mg/L and pretreated red
12~48h is impregnated in mycin solution, aseptic water washing is used after taking-up 3~5 times, and the sterile water of remained on surface is blotted with filter paper,
Wherein, entire soaking process carries out under 15~25 DEG C, dark conditions, and the pretreatment of Gibberellins solution specifically includes：By quality
Than 1:2:4 weigh acetic acid, ammonium nitrate and ammonium tartrate, and mixing stirs evenly, is then added drop-wise to Gibberellins solution while stirring
In, 2h is persistently stirred after dripping again, the total addition level of acetic acid, ammonium nitrate and ammonium tartrate is the 4% of Gibberellins solution quality；
Step 4：River sand is taken, then sterile water is added dropwise in cooled to room temperature after the 1h that sterilizes at 121 DEG C while stirring,
It is 50%~60% to water content, obtains wet sand, be in mass ratio 1:3 ratio by step 3 treated mountain fourth seed with it is wet
Sand mixing, stirs evenly, and cold stratification is then carried out at 0~5 DEG C, Stratification time is 15~30 days, in lamination process
The water content for keeping wet sand is constant.
Preferably, in step 2, the mixture of mountain sand, sodium chloride and zeolite is first pre-processed before use, in advance
Processing specifically includes：Mountain sand is weighed, is cleaned with pure water, is drained, dry 3h, is sieved after cooling, grain size is taken to be less than at 90 DEG C
The mountain sand of 2mm, weighs sodium chloride, and dry 3h, crushes, sieve with 100 mesh sieve, obtain sodium chloride fine powder, weigh zeolite, use is pure at 90 DEG C
Water purification is cleaned, and is drained, and at 100 DEG C after dry 3h, it is 2 to be immediately placed in mass ratio:1 corn oil and the miscella of sunflower oil
Middle immersion 3h takes out, and mixes with mountain sand, sodium chloride fine powder of the grain size less than 2mm, is rotated under conditions of 110 DEG C, 800r/min
Stir-frying is taken out, cooling to Zeolite dehydration.
Preferably, in step 2, what is obtained blots mountain fourth seed after rotation stir-frying processing, further includes：It presses
Mass ratio is 1:10 ratio adds in cleaning agent into mountain fourth seed, after stirring 1h, takes out mountain fourth seed, is rushed with pure water
It washes, removes the cleaning agent of remained on surface, then dry at 40 DEG C, cooling, wherein, cleaning agent is 2 by mass ratio:1:The ten of 10
Dialkyl benzene sulfonic acids sodium, ethoxylated dodecyl alcohol sodium sulphate and deionized water are mixed.
Preferably, a concentration of 300mg/L of Gibberellins solution.
Preferably, the time that mountain fourth seed impregnates in Gibberellins solution is 18h.
Preferably, the temperature of cold stratification is 5 DEG C.
Preferably, the time of cold stratification is 20 days.
The present invention includes at least following advantageous effect：
(1) method provided by the invention is used, the suspend mode of mountain fourth seed can be fast and effeciently released, greatly shorten mountain fourth
Sub- seed sprouts the required time, improves the nursery rate of mountain fourth, supply falls short of demand so as to solve the problems, such as mountain fourth seedling.
(2) method provided by the invention is used, while the time needed for the sprouting of mountain fourth seed is shortened, moreover it is possible to ensure
The germination rate of mountain fourth seed, after treatment, the germination rate of mountain fourth seed are up to 85%.
(3) in method provided by the invention, mountain fourth fruit passes through the mixing water of potassium hydroxide, sodium hydroxide and ethylene glycol
After solution impregnates, it can effectively soften, decompose pericarp, then ferment under the action of saccharomycete, pulp can be decomposed, so as to
The pericarp and pulp for making mountain fourth fruit are degenerated completely, finally with high pressure water washing, so that it may so that pericarp and pulp are completely from kind
It comes off on skin, and the mixed liquor of pectase and water can decompose the pectin that seed coat surface is sticked, and handle mountain fourth in this way
During sub- fruit, compared to the mode of traditional direct mountain fourth fruit is soaked in water corruption or accumulation corruption, have higher
Processing speed and treatment effect, while manpower burden can be mitigated.
(4) in method provided by the invention, mountain fourth seed rotates stir-frying in the mixture of mountain sand, sodium chloride and zeolite
When, can effectively grind off kind of a skin, benevolence is made fully to be exposed, enhance the permeability of seed, enable gibberellin effectively into
Enter and play a role inside benevolence, the suspend mode of mountain fourth seed is released, in addition, the mixture of mountain sand, sodium chloride and zeolite is using
Preceding first to be pre-processed, the miscella adsorbed in the sodium chloride fine powder and zeolite obtained after pretreatment can function well as
Lubricating action protects benevolence, prevents benevolence from being damaged during seed grinds off kind of skin.
(5) in method provided by the invention, acetic acid, ammonium nitrate and tartaric acid are also added in Gibberellins solution used
Ammonium, three have synergistic effect, can enhance the effect that gibberellin releases mountain fourth seed dormancy, so as to Rapid reversal mountain fourth
The sprouting of mountain fourth seed is accelerated in the suspend mode of seed.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
With reference to example, the present invention is described in further detail, to enable those skilled in the art with reference to specification text
Word can be implemented according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other element or combinations.
<Embodiment 1>
The method of Rapid reversal mountain fourth seed dormancy includes：By mountain fourth fruit be put into pH be 10, mass ratio 2:2:1
Potassium hydroxide, sodium hydroxide and ethylene glycol mixed aqueous solution in, impregnate for 24 hours, take out at 40 DEG C, 3 are rinsed with pure water
It is secondary, it is then placed in the acetum that pH is 5.0, while add in saccharomycete, ferment 18h at 25 DEG C, takes out, and obtains the mountain of fermentation
The sub- fruit of fourth, then the flow for being 6MPa with pressure rinse the mountain fourth fruit of fermentation, until pericarp, pulp and seed divide completely
From obtaining mountain fourth seed, mountain fourth seed be put into mass ratio for 1:In 15 pectase and the mixed liquor of water, stirred at 40 DEG C
It mixes for 24 hours, filtering, the remaining impurity of mountain fourth the surface of the seed after washing out filtering with pure water, and remained on surface is blotted with filter paper
Pure water, mountain fourth seed must be blotted, wherein, pH is first adjusted to 3.5 by the mixed liquor of pectase and water using preceding with hydrochloric acid；It will
The obtained mountain fourth seed that blots is added to mass ratio as 2:2:In the mixture of 1 mountain sand, sodium chloride and zeolite, stirring is equal
It is even, 40 DEG C, rotation stir-frying 1h under conditions of 2000r/min are placed in, cooling isolates mountain fourth seed, the mountain fourth that will be isolated
Sub- seed, which is placed in the liquor potassic permanganate that mass fraction is 0.1%, impregnates 1h, after taking-up, with aseptic water washing 3 times, is placed in the moon
Liang Chu dries；Treated mountain fourth seed is put into a concentration of 100mg/L and pretreated Gibberellins solution and is soaked
48h is steeped, aseptic water washing is used after taking-up 3 times, and the sterile water of remained on surface is blotted with filter paper, wherein, entire soaking process exists
15 DEG C, carry out under dark conditions, the pretreatment of Gibberellins solution specifically includes：In mass ratio 1:2:4 weigh acetic acid, ammonium nitrate and
Ammonium tartrate, mixing, stirs evenly, is then added drop-wise in Gibberellins solution while stirring, persistently stir 2h, vinegar after dripping again
The total addition level of acid, ammonium nitrate and ammonium tartrate is the 4% of Gibberellins solution quality；River sand is taken, after the 1h that sterilizes at 121 DEG C certainly
It is so cooled to room temperature, sterile water is then added dropwise while stirring, until water content is 50%, obtains wet sand, be in mass ratio 1:3 ratio
By step 3, treated that mountain fourth seed is mixed with wet sand, stirs evenly, cold stratification, layer are then carried out at 0 DEG C
The product time is 15 days, keeps the water content of wet sand constant in lamination process.
Wherein, the mixture of mountain sand, sodium chloride and zeolite is first pre-processed before use, and pretreatment specifically includes：Claim
Mountain sand is taken, is cleaned with pure water, is drained, dry 3h, is sieved after cooling at 90 DEG C, and the mountain sand that grain size is taken to be less than 2mm weighs chlorine
Changing sodium, dry 3h, crushes, sieves with 100 mesh sieve, obtain sodium chloride fine powder, weigh zeolite, cleaned with pure water, drained at 90 DEG C, in
At 100 DEG C after dry 3h, it is 2 to be immediately placed in mass ratio:3h is impregnated in 1 corn oil and the miscella of sunflower oil, is taken out, with
Mountain sand of the grain size less than 2mm, the mixing of sodium chloride fine powder, rotate under conditions of 110 DEG C, 800r/min and stir-fry to Zeolite dehydration,
It takes out, cooling.What is obtained blots mountain fourth seed after rotation stir-frying processing, further includes：It is 1 in mass ratio:10 ratio
Cleaning agent is added in into mountain fourth seed, after stirring 1h, mountain fourth seed is taken out, is rinsed with pure water, remove remained on surface
Cleaning agent, then dry at 40 DEG C, cooling, wherein, cleaning agent is 2 by mass ratio:1:10 neopelex, the moon
Cinnamic alcohol polyethenoxy ether sodium sulfate and deionized water are mixed.
Mountain fourth seed sprouting processing includes：First the mountain fourth seed after cold stratification is rinsed 3 times with sterile water,
It is subsequently placed in 25 DEG C of constant temperature illumination box, sprouting culture is carried out with filter paper germinating bed, wherein, mountain fourth seed amount is
300, it is divided into 3 parts, repeats the germination rate of statistics mountain fourth seed after experiment 3 times, 100 every time, 30 days.
<Embodiment 2>
The method of Rapid reversal mountain fourth seed dormancy includes：By mountain fourth fruit be put into pH be 10, mass ratio 2:2:1
Potassium hydroxide, sodium hydroxide and ethylene glycol mixed aqueous solution in, impregnate for 24 hours, take out at 40 DEG C, 3 are rinsed with pure water
It is secondary, it is then placed in the acetum that pH is 5.0, while add in saccharomycete, ferment 18h at 25 DEG C, takes out, and obtains the mountain of fermentation
The sub- fruit of fourth, then the flow for being 6MPa with pressure rinse the mountain fourth fruit of fermentation, until pericarp, pulp and seed divide completely
From obtaining mountain fourth seed, mountain fourth seed be put into mass ratio for 1:In 15 pectase and the mixed liquor of water, stirred at 40 DEG C
It mixes for 24 hours, filtering, the remaining impurity of mountain fourth the surface of the seed after washing out filtering with pure water, and remained on surface is blotted with filter paper
Pure water, mountain fourth seed must be blotted, wherein, pH is first adjusted to 3.5 by the mixed liquor of pectase and water using preceding with hydrochloric acid；It will
The obtained mountain fourth seed that blots is added to mass ratio as 2:2:In the mixture of 1 mountain sand, sodium chloride and zeolite, stirring is equal
It is even, 40 DEG C, rotation stir-frying 1h under conditions of 2000r/min are placed in, cooling isolates mountain fourth seed, the mountain fourth that will be isolated
Sub- seed, which is placed in the liquor potassic permanganate that mass fraction is 0.1%, impregnates 1h, after taking-up, with aseptic water washing 5 times, is placed in the moon
Liang Chu dries；Treated mountain fourth seed is put into a concentration of 300mg/L and pretreated Gibberellins solution and is soaked
18h is steeped, aseptic water washing is used after taking-up 5 times, and the sterile water of remained on surface is blotted with filter paper, wherein, entire soaking process exists
20 DEG C, carry out under dark conditions, the pretreatment of Gibberellins solution specifically includes：In mass ratio 1:2:4 weigh acetic acid, ammonium nitrate and
Ammonium tartrate, mixing, stirs evenly, is then added drop-wise in Gibberellins solution while stirring, persistently stir 2h, vinegar after dripping again
The total addition level of acid, ammonium nitrate and ammonium tartrate is the 4% of Gibberellins solution quality；River sand is taken, after the 1h that sterilizes at 121 DEG C certainly
It is so cooled to room temperature, sterile water is then added dropwise while stirring, until water content is 60%, obtains wet sand, be in mass ratio 1:3 ratio
By step 3, treated that mountain fourth seed is mixed with wet sand, stirs evenly, cold stratification, layer are then carried out at 5 DEG C
The product time is 20 days, keeps the water content of wet sand constant in lamination process.
Wherein, the mixture of mountain sand, sodium chloride and zeolite is first pre-processed before use, and pretreatment specifically includes：Claim
Mountain sand is taken, is cleaned with pure water, is drained, dry 3h, is sieved after cooling at 90 DEG C, and the mountain sand that grain size is taken to be less than 2mm weighs chlorine
Changing sodium, dry 3h, crushes, sieves with 100 mesh sieve, obtain sodium chloride fine powder, weigh zeolite, cleaned with pure water, drained at 90 DEG C, in
At 100 DEG C after dry 3h, it is 2 to be immediately placed in mass ratio:3h is impregnated in 1 corn oil and the miscella of sunflower oil, is taken out, with
Mountain sand of the grain size less than 2mm, the mixing of sodium chloride fine powder, rotate under conditions of 110 DEG C, 800r/min and stir-fry to Zeolite dehydration,
It takes out, cooling.What is obtained blots mountain fourth seed after rotation stir-frying processing, further includes：It is 1 in mass ratio:10 ratio
Cleaning agent is added in into mountain fourth seed, after stirring 1h, mountain fourth seed is taken out, is rinsed with pure water, remove remained on surface
Cleaning agent, then dry at 40 DEG C, cooling, wherein, cleaning agent is 2 by mass ratio:1:10 neopelex, the moon
Cinnamic alcohol polyethenoxy ether sodium sulfate and deionized water are mixed.
Mountain fourth seed sprouting processing includes：First the mountain fourth seed after cold stratification is rinsed 3 times with sterile water,
It is subsequently placed in 25 DEG C of constant temperature illumination box, sprouting culture is carried out with filter paper germinating bed, wherein, mountain fourth seed amount is
300, it is divided into 3 parts, repeats the germination rate of statistics mountain fourth seed after experiment 3 times, 100 every time, 30 days.
<Embodiment 3>
The method of Rapid reversal mountain fourth seed dormancy includes：By mountain fourth fruit be put into pH be 10, mass ratio 2:2:1
Potassium hydroxide, sodium hydroxide and ethylene glycol mixed aqueous solution in, impregnate for 24 hours, take out at 40 DEG C, 3 are rinsed with pure water
It is secondary, it is then placed in the acetum that pH is 5.0, while add in saccharomycete, ferment 18h at 25 DEG C, takes out, and obtains the mountain of fermentation
The sub- fruit of fourth, then the flow for being 6MPa with pressure rinse the mountain fourth fruit of fermentation, until pericarp, pulp and seed divide completely
From obtaining mountain fourth seed, mountain fourth seed be put into mass ratio for 1:In 15 pectase and the mixed liquor of water, stirred at 40 DEG C
It mixes for 24 hours, filtering, the remaining impurity of mountain fourth the surface of the seed after washing out filtering with pure water, and remained on surface is blotted with filter paper
Pure water, mountain fourth seed must be blotted, wherein, pH is first adjusted to 3.5 by the mixed liquor of pectase and water using preceding with hydrochloric acid；It will
The obtained mountain fourth seed that blots is added to mass ratio as 2:2:In the mixture of 1 mountain sand, sodium chloride and zeolite, stirring is equal
It is even, 40 DEG C, rotation stir-frying 1h under conditions of 2000r/min are placed in, cooling isolates mountain fourth seed, the mountain fourth that will be isolated
Sub- seed, which is placed in the liquor potassic permanganate that mass fraction is 0.1%, impregnates 1h, after taking-up, with aseptic water washing 4 times, is placed in the moon
Liang Chu dries；Treated mountain fourth seed is put into a concentration of 500mg/L and pretreated Gibberellins solution and is soaked
12h is steeped, aseptic water washing is used after taking-up 4 times, and the sterile water of remained on surface is blotted with filter paper, wherein, entire soaking process exists
25 DEG C, carry out under dark conditions, the pretreatment of Gibberellins solution specifically includes：In mass ratio 1:2:4 weigh acetic acid, ammonium nitrate and
Ammonium tartrate, mixing, stirs evenly, is then added drop-wise in Gibberellins solution while stirring, persistently stir 2h, vinegar after dripping again
The total addition level of acid, ammonium nitrate and ammonium tartrate is the 4% of Gibberellins solution quality；River sand is taken, after the 1h that sterilizes at 121 DEG C certainly
It is so cooled to room temperature, sterile water is then added dropwise while stirring, until water content is 55%, obtains wet sand, be in mass ratio 1:3 ratio
By step 3, treated that mountain fourth seed is mixed with wet sand, stirs evenly, cold stratification, layer are then carried out at 3 DEG C
The product time is 30 days, keeps the water content of wet sand constant in lamination process.
Wherein, the mixture of mountain sand, sodium chloride and zeolite is first pre-processed before use, and pretreatment specifically includes：Claim
Mountain sand is taken, is cleaned with pure water, is drained, dry 3h, is sieved after cooling at 90 DEG C, and the mountain sand that grain size is taken to be less than 2mm weighs chlorine
Changing sodium, dry 3h, crushes, sieves with 100 mesh sieve, obtain sodium chloride fine powder, weigh zeolite, cleaned with pure water, drained at 90 DEG C, in
At 100 DEG C after dry 3h, it is 2 to be immediately placed in mass ratio:3h is impregnated in 1 corn oil and the miscella of sunflower oil, is taken out, with
Mountain sand of the grain size less than 2mm, the mixing of sodium chloride fine powder, rotate under conditions of 110 DEG C, 800r/min and stir-fry to Zeolite dehydration,
It takes out, cooling.What is obtained blots mountain fourth seed after rotation stir-frying processing, further includes：It is 1 in mass ratio:10 ratio
Cleaning agent is added in into mountain fourth seed, after stirring 1h, mountain fourth seed is taken out, is rinsed with pure water, remove remained on surface
Cleaning agent, then dry at 40 DEG C, cooling, wherein, cleaning agent is 2 by mass ratio:1:10 neopelex, the moon
Cinnamic alcohol polyethenoxy ether sodium sulfate and deionized water are mixed.
Mountain fourth seed sprouting processing includes：First the mountain fourth seed after cold stratification is rinsed 3 times with sterile water,
It is subsequently placed in 25 DEG C of constant temperature illumination box, sprouting culture is carried out with filter paper germinating bed, wherein, mountain fourth seed amount is
300, it is divided into 3 parts, repeats the germination rate of statistics mountain fourth seed after experiment 3 times, 100 every time, 30 days.
In order to illustrate the effect of the present invention, it is as follows that inventor provides comparative experiments：
<Comparative example 1>
It obtains blotting mountain fourth from mountain fourth fruit according to the method for Rapid reversal mountain fourth seed dormancy in embodiment 2
After seed, subsequent processing is no longer carried out, sprouting processing is directly carried out to mountain fourth seed, the sprouting processing of mountain fourth seed is the same as real
Apply example 2.
<Comparative example 2>
After mountain fourth fruit directly is impregnated 123h into the water, after removing pericarp and pulp with hand rubbing, pure water is used
The impurity on surface is washed out, and the pure water of remained on surface is blotted with filter paper, obtains blotting mountain fourth seed, then to blotting mountain fourth
Sub- seed carries out subsequent processing, and method for subsequent processing is the same as embodiment 2.
The sprouting of mountain fourth seed is handled with embodiment 2.
<Comparative example 3>
The method of Rapid reversal mountain fourth seed dormancy with embodiment 2, the difference lies in：Gibberellins solution is not carried out
Pretreatment.
The sprouting of mountain fourth seed is handled with embodiment 2.
<Comparative example 4>
The method of Rapid reversal mountain fourth seed dormancy with embodiment 2, the difference lies in：Not to mountain sand, sodium chloride and
The mixture of zeolite is pre-processed, and is directly used.
The sprouting of mountain fourth seed is handled with embodiment 2.
After measured, the germination rate of mountain fourth seed such as the following table 1：
[table 1]
From upper table 1, it can be seen that the germination rate of Examples 1 to 3 is more than 75%, illustrate the side that Examples 1 to 3 provides
Method can fast and effeciently release the suspend mode of mountain fourth seed, make mountain fourth seed that can just sprout in a short time, and implement
The method that example 1~3 provides will not cause to damage to mountain fourth seed, can guarantee that mountain fourth seed remains to normally sprout.Wherein, it is real
The germination rate highest of example 2 is applied, is most preferred embodiment.
Compared with Example 2, the sprouting of mountain fourth seed processing is identical for comparative example 1, only according to quickly being solved in embodiment 2
Except the method for mountain fourth seed dormancy is obtained from mountain fourth fruit after blotting mountain fourth seed, subsequent processing is no longer carried out, directly
It connects and sprouting processing is carried out to mountain fourth seed.From upper table 1, it can be seen that the germination rate of comparative example 1 is 0, illustrate without releasing
The mountain fourth seed of suspend mode processing can not be sprouted in a short time.This is because mountain fourth seed is there are apparent dormancy,
Breaking dormancy in its natural state, the time needed are up to 6 months, and after breaking dormancy is handled, it can be by mountain fourth seed
Rest period shorten to 20 days or so, therefore can make mountain fourth seed fast germination after carrying out sprouting processing.
Comparative example 2 compared with Example 2, after mountain fourth fruit directly is impregnated 123h into the water, is removed with hand rubbing
After pericarp and pulp, the impurity on surface is washed out with pure water, and the pure water of remained on surface is blotted with filter paper, obtains blotting mountain fourth
Sub- seed, then carries out subsequent processing to blotting mountain fourth seed, and method for subsequent processing is same as Example 2.Mountain fourth seed
Sprouting processing it is identical with embodiment 2.From upper table 1, it can be seen that the germination rate of comparative example 2 compared with Example 2
It reduces.This is because when directly rubbing fruit with hand, seed can be made by a degree of damage and pollution, and cannot remove
The pectin sticked on kind skin, pectin remain on kind of skin the effect that gibberellin releases mountain fourth seed dormancy that can reduce, simultaneously also
The sprouting of mountain fourth seed can be hindered, is born in addition, manpower can be greatly increased with hand rubbing fruit, pollution working environment, and mountain
The sub- fruit of fourth can effectively soften after the mixed aqueous solution of potassium hydroxide, sodium hydroxide and ethylene glycol impregnates, decompose pericarp,
Then it ferments under the action of saccharomycete, pulp can be decomposed, so as to make the pericarp of mountain fourth fruit and pulp completely rotten
Change, finally with high pressure water washing, so that it may so that pericarp and pulp come off from kind of skin completely, and the mixed liquor of pectase and water
The pectin that seed coat surface is sticked can be decomposed, when handling mountain fourth fruit in this way, compared to directly by mountain fourth fruit
Be soaked in water the mode of corruption, has higher processing speed and treatment effect, and can also purify working environment, mitigates manpower
Burden.
Compared with Example 2, the method for Rapid reversal mountain fourth seed dormancy is identical for comparative example 3, the difference lies in：Not yet
Have and Gibberellins solution is pre-processed.The sprouting processing of mountain fourth seed is identical with embodiment 2.It can from upper table 1
Go out, the germination rate of comparative example 3 compared with Example 2, there is larger reduction, illustrates that the method that comparative example 3 is provided is releasing
Effect in mountain fourth seed dormancy decreases.This is because be added in the pretreatment of gibberellin acetic acid, ammonium nitrate and
Ammonium tartrate, three have synergistic effect, can enhance the effect that gibberellin inducement generates hydrolase, make to store in seed has
Machine object is decomposed into small molecule by macromolecular, is utilized by embryo, promotes embryo after-ripening, stops so as to enhance gibberellin releasing mountain fourth seed
The effect of dormancy fast and effeciently releases the suspend mode of mountain fourth seed, accelerates the sprouting of mountain fourth seed.
Compared with Example 2, the method for Rapid reversal mountain fourth seed dormancy is identical for comparative example 4, the difference lies in：Not yet
Have and the mixture of mountain sand, sodium chloride and zeolite is pre-processed, and directly use.The sprouting processing and implementation of mountain fourth seed
Example 2 is identical.From upper table 1, it can be seen that the germination rate of comparative example 4 compared with Example 2, has and is greatly lowered, explanation
Effect of the method that comparative example 4 is provided in mountain fourth seed dormancy is released, which has, to be greatly lowered.This is because on the one hand,
The mountain sand that zeolite and the grain size chosen by pretreatment are less than 2mm can grind off kind of a skin well, enhance the permeability of seed, make
Gibberellin can be efficiently entering inside benevolence and play a role, and shorten the rest period of mountain fourth seed；On the other hand, after pretreatment
To sodium chloride fine powder and zeolite in the miscella that adsorbs can function well as lubricating action, protect benevolence, prevent benevolence
It is damaged during seed grinds off kind of skin, so as to ensure that mountain fourth seed can normally be sprouted.
Although the embodiments of the present invention have been disclosed as above, but its be not restricted in specification and embodiment it is listed
With it can be applied to various suitable the field of the invention completely.It for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, it is of the invention and unlimited
In specific details and example shown and described herein.
Claims (7)
1. the method for Rapid reversal mountain fourth seed dormancy, which is characterized in that include the following steps：
Step 1：By mountain fourth fruit be put into pH be 10, mass ratio 2:2:1 potassium hydroxide, sodium hydroxide and ethylene glycol
It in mixed aqueous solution, impregnates for 24 hours, takes out at 40 DEG C, rinsed 3 times with pure water, be then placed in the acetum that pH is 5.0
In, while saccharomycete is added in, ferment 18h at 25 DEG C, takes out, and obtains the mountain fourth fruit of fermentation, then the water for being 6MPa with pressure
Stream rinses the mountain fourth fruit of fermentation, until pericarp, pulp and seed are kept completely separate, mountain fourth seed is obtained, by mountain fourth seed
It is 1 to be put into mass ratio:It in 15 pectase and the mixed liquor of water, is stirred at 40 DEG C for 24 hours, filtering washes out filtering with pure water
The remaining impurity of mountain fourth the surface of the seed afterwards, and the pure water of remained on surface is blotted with filter paper, mountain fourth seed must be blotted,
In, pH is first adjusted to 3.5 by the mixed liquor of pectase and water using preceding with hydrochloric acid；
Step 2：The mountain fourth seed that blots that step 1 obtains is added to mass ratio as 2:2:1 mountain sand, sodium chloride and zeolite
Mixture in, stir evenly, be placed in 40 DEG C, rotation stir-frying 1h under conditions of 2000r/min, mountain fourth kind is isolated in cooling
The mountain fourth seed isolated is placed in the liquor potassic permanganate that mass fraction is 0.1% and impregnates 1h, after taking-up, with nothing by son
Bacterium water rinses 3~5 times, In Shade to dry；
Step 3：By step 2, treated that mountain fourth seed is put into a concentration of 100~500mg/L and pretreated gibberellin
12~48h is impregnated in solution, aseptic water washing is used after taking-up 3~5 times, and the sterile water of remained on surface is blotted with filter paper, wherein,
Entire soaking process carries out under 15~25 DEG C, dark conditions, and the pretreatment of Gibberellins solution specifically includes：In mass ratio 1:2:
4 weigh acetic acid, ammonium nitrate and ammonium tartrate, and mixing is stirred evenly, is then added drop-wise in Gibberellins solution while stirring, is added dropwise
2h is persistently stirred after complete again, the total addition level of acetic acid, ammonium nitrate and ammonium tartrate is the 4% of Gibberellins solution quality；
Step 4：River sand is taken, then sterile water is added dropwise, until containing in cooled to room temperature after the 1h that sterilizes at 121 DEG C while stirring
Water is 50%~60%, obtains wet sand, is in mass ratio 1:Treated that mountain fourth seed is mixed with wet sand by step 3 for 3 ratio
It closes, stirs evenly, cold stratification is then carried out at 0~5 DEG C, Stratification time is 15~30 days, is kept in lamination process
The water content of wet sand is constant.
2. the method for Rapid reversal mountain fourth seed dormancy as described in claim 1, which is characterized in that in step 2, mountain
The mixture of sand, sodium chloride and zeolite is first pre-processed before use, and pretreatment specifically includes：Mountain sand is weighed, uses pure water
It cleans, drains, dry 3h, is sieved after cooling at 90 DEG C, and the mountain sand that grain size is taken to be less than 2mm weighs sodium chloride, is done at 90 DEG C
Dry 3h is crushed, is sieved with 100 mesh sieve, obtain sodium chloride fine powder, weigh zeolite, cleaned with pure water, drained, the dry 3h at 100 DEG C
Afterwards, it is 2 to be immediately placed in mass ratio:3h is impregnated in 1 corn oil and the miscella of sunflower oil, is taken out, with grain size less than 2mm's
Mountain sand, the mixing of sodium chloride fine powder, rotation stir-frying to Zeolite dehydration, taking-up cools down under conditions of 110 DEG C, 800r/min.
3. the method for Rapid reversal mountain fourth seed dormancy as claimed in claim 2, which is characterized in that in step 2, obtain
To blot mountain fourth seed by rotation stir-frying processing after, further include：It is 1 in mass ratio:10 ratio is to mountain fourth seed
Middle addition cleaning agent after stirring 1h, takes out mountain fourth seed, is rinsed with pure water, remove the cleaning agent of remained on surface, Ran Houyu
It is dry at 40 DEG C, cooling, wherein, cleaning agent is 2 by mass ratio:1:10 neopelex, lauryl alcohol polyethylene glycol oxide
Ether sodium sulfate and deionized water are mixed.
4. the method for Rapid reversal mountain fourth seed dormancy as described in claim 1, which is characterized in that Gibberellins solution it is dense
It spends for 300mg/L.
5. the method for Rapid reversal mountain fourth seed dormancy as claimed in claim 4, which is characterized in that mountain fourth seed is red
The time impregnated in mycin solution is 18h.
6. the method for Rapid reversal mountain fourth seed dormancy as claimed in claim 5, which is characterized in that cold stratification
Temperature is 5 DEG C.
7. the method for Rapid reversal mountain fourth seed dormancy as claimed in claim 6, which is characterized in that cold stratification
Time is 20 days.
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Cited By (1)
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| CN111528072A (en) * | 2020-06-08 | 2020-08-14 | 武汉市农业科学院 | Kiwi fruit sowing and seedling raising method |
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