CN104285542B - A kind of magnetic treatment promotes the method that Nanjing linden seeds germinates - Google Patents
A kind of magnetic treatment promotes the method that Nanjing linden seeds germinates Download PDFInfo
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- CN104285542B CN104285542B CN201410611943.8A CN201410611943A CN104285542B CN 104285542 B CN104285542 B CN 104285542B CN 201410611943 A CN201410611943 A CN 201410611943A CN 104285542 B CN104285542 B CN 104285542B
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- 238000000034 method Methods 0.000 title claims abstract description 36
- 240000007313 Tilia cordata Species 0.000 title claims abstract description 18
- 239000003651 drinking water Substances 0.000 claims abstract description 29
- 235000020188 drinking water Nutrition 0.000 claims abstract description 29
- 238000005530 etching Methods 0.000 claims abstract description 19
- 230000005059 dormancy Effects 0.000 claims abstract description 18
- 230000035784 germination Effects 0.000 claims abstract description 17
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000003475 lamination Methods 0.000 claims abstract description 16
- 239000002253 acid Substances 0.000 claims abstract description 15
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 14
- 239000003448 gibberellin Substances 0.000 claims abstract description 14
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims abstract description 13
- 238000002791 soaking Methods 0.000 claims abstract description 13
- 230000007226 seed germination Effects 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 9
- 239000011521 glass Substances 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 239000004576 sand Substances 0.000 claims description 6
- 238000013517 stratification Methods 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000002054 transplantation Methods 0.000 claims description 3
- 238000003672 processing method Methods 0.000 claims description 2
- 241000328973 Tilia miqueliana Species 0.000 abstract description 11
- 238000011161 development Methods 0.000 abstract description 3
- 230000018109 developmental process Effects 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 2
- 230000004071 biological effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000219071 Malvaceae Species 0.000 description 2
- 241000907897 Tilia Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 238000009738 saturating Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 244000292839 Ficus religiosa Species 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000005058 diapause Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 230000005415 magnetization Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 230000014284 seed dormancy process Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a kind of method that magnetic treatment promotes that Nanjing linden seeds germinates, to Nanjing linden seeds concentrated sulfuric acid etching time, Gibberellins solution seed soaking time, in low temperature lamination process, magnetized drinking water is processed the conditions such as intensity and frequency and has been carried out strict control, shell by seed, concentrated sulfuric acid acid etching, Gibberellins solution seed soaking, magnetized drinking water processing, low temperature lamination, germination environment control step realizes, due to the control of having strengthened key link in Nanjing linden seeds breaking dormancy and germination process, Biological Effect of Magnetic Fields is applied to seed breaking dormancy and germination process, impel seed breaking dormancy and sprouting rapidly, reach sprouting speed fast, sprout neat, the high texts of planting percent, solve a Nanjing linden seeds reproduction technique difficult problem, full seed germination percentage reaches more than 90%, planting percent exceedes 85%, establish good seedling basis for realizing the industrialized development of tilia miqueliana cultivating seedlings.
Description
Technical field
The present invention relates to achievements in forest-tree seedling breeding field, be specifically related to a kind of method that magnetic treatment promotes that Nanjing linden seeds germinates.
Background technology
Tilia miqueliana (TiliamiquelianaMaxim.) has another name called close gram of linden, white passwood, is commonly called as " bodhi tree ", is Tiliaceae (Tiliaceae)Tilia (TiliaL.) seeds, China's endemic tree. The strong fragrance of the tilia miqueliana fragrance of a flower, the large shade of leaf is dense, trunk is tall and straight, collection afforestation,Medicinal, material with, nectariferous plant, because of its mysterious religion culture color, once by a large amount of introducing and plantings of Japan. Tilia miquelianaOnce for East China indigenous tree extensively distributes, but due to breeding difficulty and habitat destruction, its population quantity and individual amount areCarrying out reproduction technique research becomes the task of top priority of tilia miqueliana protection of resources, development and utilization through decay gradually!
Tilia miqueliana reproduction technique is taking seminal propagation as main, but Nanjing linden seeds plumpness is low and deep dormancy characteristic to have a strong impact on nursery stock numerousGrow, become the bottleneck problem that suppresses tilia miqueliana seeding propagation. At present, be also applied to without Nanjing linden seeds reproduction technique both at home and abroadThe report of nursery stock production, its main cause is that Nanjing linden seeds plumpness is low, seed breaking dormancy difficulty, seed germination seedlingRate is low, often causes growing seedlings unsuccessfully.
Nanjing Forestry University, from 2003, carries out systematicness, continuation to contents such as tilia miqueliana germ plasm resource, reproduction techniquesScientific research, the exploitation of study emphasis reproduction technique, has successively carried out the repeatedly research of tilia miqueliana seed propagation technology. Section for many yearsMajor in and close very difficulty of confirmation tilia miqueliana seedling propagation, obtain high quality seedling and also realize very difficulty of Industrialization of seeds and seedlings.
Through repeatedly attempting, and improve gradually experimental technique, Nanjing linden seeds reproduction technique makes a breakthrough, and succeeds. MagneticChange the method that water treatment promotes that Nanjing linden seeds germinates, comprise to seed selections, acid etching seed, Seeds Treated with Magnetic Field, hormone soak seed,The strict controls of condition such as lamination processing, germination, by wet concentration full seed, concentrated sulfuric acid acid etching seed, magnetized drinking water processingThe management process such as seed, Gibberellins solution seed soaking, cold stratification, intensification germination are realized, and have strengthened Nanjing linden seedsThe control of key link in termination of diapause and germination process, wet concentration seed has improved seed quality, and acid etching seed is conducive to increase plantsThe saturating property of skin and minimizing mechanical obstacles, gibberellin seed soaking is conducive to break seed dormancy, and magnetic treatment is conducive to seed breaking dormancy and shortEnter to sprout, integrated treatment optimized seed breaking dormancy, promote the environment sprouted, ensure seed can be fast, neatly sprout,Establish good seedling basis for realizing the industrialized development of tilia miqueliana cultivating seedlings.
Summary of the invention
The invention provides a kind of method that magnetic treatment promotes that Nanjing linden seeds germinates, to wet concentration seed, acid etching seed, magnetic treatmentSeed, hormone seed soaking, low temperature lamination and intensification germination condition have carried out strict control, have obtained splendid effect.
The present invention realizes by following technical solution:
Magnetic treatment promotes the method that Nanjing linden seeds germinates, and concrete steps comprise:
(1) wet concentration full seed: use hawkbill to be caught broken shell, seed is taken out, note not clipping broken seed coat, some are empty grain obviouslyOr damaged seed should be rejected in time. The seed that will shell is put into washbowl or bulk container, and glass bar fully stirs; After 30min,The seed that floats on the water surface is removed, be sunken to bottom seed and take out and drain moisture, this part seed is full seed; By fullSeed is placed on 3～5 layers of newspaper or blotting paper, and paper using blots the surface of the seed moisture.
(2) concentrated sulfuric acid acid etching seed: 100～150g seed is placed in to 1000ml beaker, adds the concentrated sulfuric acid of 250～300ml;Concentrated sulfuric acid etching time is 40～50min, and during this time, glass bar continues slowly to stir; The concentrated sulfuric acid in beaker is slowly inclined and, acidSeed after erosion uses running water fully to clean, and flowing water rinses 24h.
(3) magnetized drinking water is processed seed: in seed breaking dormancy and germination process, all use magnetized drinking water to process seed.
Affiliated magnetized drinking water is processed seed: 1) Gibberellins solution is used the preparation of 10000～15000GS magnetized drinking water; 2) lamination mistakeIn journey, moisture is provided by 10000～15000GS magnetized drinking water; 3) in germination process, moisture magnetizes water extraction by 10000～15000GSSupply.
(4) Gibberellins solution seed soaking: the seed 48～60h that uses the Gibberellins solution of 1000～1500mg/L to soak seed after acid etching;
(5) cold stratification: the seed after seed soaking is placed in to wet rough sand, and planting husky volume ratio is 1: 3, puts into 0～5 DEG C of environmentIn carry out cold stratification; Rough sand adds magnetized drinking water, and addition is advisable with " hold agglomerating, unclamp loose ". In lamination process,Stir seed once every 5 days, and according to circumstances suitably add magnetized drinking water. After lamination 50～60 days, seed is that breaking dormancy entersEnter germinating.
(6) heat up and germinate: the seed that enters germinating is transferred in 23 ± 2 DEG C of illumination boxs to intensity of illumination by low temperature environment10000～15000LX, light application time 10～12h/ days, observes sprouting situation next day of duration of germination, add in time magnetized drinking water, straightAfter launching completely to cotyledon, carry out bud transplantation of seedlings.
With this processing method, seed germination rate can reach 90% level, transplants planting percent and exceedes 85%.
The advantage of technical solution of the present invention is:
(1) wet concentration full seed has improved seed quality, by sky grain and grow imperfect seed and reject, has reduced seed subsequent treatmentQuantity, has improved seed germination rate, meanwhile, has reduced in earthing of seeds process rotten probability occurs.
(2) concentrated sulfuric acid acid etching seed, has increased the saturating property of seed coat, has reduced the mechanical obstacles of seed coat, is conducive to seed breaking dormancy and sproutsSend out, meanwhile, concentrated sulfuric acid acid etching can also play to seed coat surface the effect of sterilization.
(3) Gibberellins solution seed soaking, has improved Interior Seed GA/ABA ratio, can indirectly shorten the low temperature lamination time, favourableSprout in seed breaking dormancy.
(4) low temperature lamination, can promote seed to remove physiological dormancy, promotes seed germination, improves seed germination regularity.
(5) Seeds Treated with Magnetic Field, multiple links of seed treatment are used magnetized drinking water, make all to make in seed breaking dormancy and germination processWith magnetic treatment, magnetic treatment be conducive to seed absorb moisture, increase respiratory intensity, promote Interior Seed physiological activator to increase andImprove related enzyme activity, promote seed breaking dormancy and sprouting.
Detailed description of the invention
Now adopt following embodiment to be further described the present invention, be not restricted to following reality but invent related scopeExecute example.
Embodiment 1
Magnetic treatment promotes the method that Nanjing linden seeds germinates, and concrete steps comprise:
1, seed shells
Use hawkbill to be caught broken shell, seed taken out, note not clipping broken seed coat, some obviously empty grain or damaged seed should andIn time, rejects.
2, wet concentration full seed
The seed that will shell is put into washbowl or bulk container, and glass bar fully stirs; After 30min, the seed that floats on the water surface is goneRemove, be sunken to bottom seed and take out and drain moisture, this part seed is full seed; By full seed be placed in 3～5 layers of newspaper orOn blotting paper, and paper using blots the surface of the seed moisture.
3, concentrated sulfuric acid acid etching seed
100～150g seed is placed in to 1000ml beaker, adds the concentrated sulfuric acid of 250～300ml; Concentrated sulfuric acid etching time is40～50min, during this time, glass bar continues slowly to stir; The concentrated sulfuric acid in beaker is slowly inclined and, and the seed after acid etching uses from the beginningWater fully cleans, and flowing water rinses 24h.
4, magnetized drinking water is processed seed
In seed breaking dormancy and germination process, all use magnetized drinking water to process seed. Magnetized drinking water is processed seed and is divided into three links:Gibberellins solution is used the preparation of 10000～15000GS magnetized drinking water; In lamination process, moisture is by 10000～15000GS magnetization water extractionSupply; In germination process, moisture is provided by 10000～15000GS magnetized drinking water.
5, Gibberellins solution seed soaking
Seed 48～the 60h that uses the Gibberellins solution of 1000～1500mg/L to soak seed after acid etching, stirs in seed soaking process frequently,Make the abundant absorbent solution of seed.
6, cold stratification
Seed after seed soaking is placed in to wet rough sand, and planting husky volume ratio is 1: 3, puts into 0～5 DEG C of environment and carries out low temperature lamination placeReason; Rough sand adds magnetized drinking water, and addition is advisable with " hold agglomerating, unclamp loose ". In lamination process, stirred and plant every 5 daysSon once, and is according to circumstances suitably added magnetized drinking water. After lamination 50～60 days, seed is that breaking dormancy enters germinating.
7, heat up and germinate
The seed that enters germinating is transferred in 23 ± 2 DEG C of illumination boxs by low temperature environment, intensity of illumination 10000～15000LX, light application time 10～12h/ days, observes sprouting situation next day of duration of germination, add in time magnetized drinking water, until cotyledonAfter launching completely, carry out bud transplantation of seedlings.
Claims (1)
1. magnetic treatment promotes the method that Nanjing linden seeds germinates, and it is characterized in that, adopts following steps:
(1) wet concentration full seed: use hawkbill to be caught broken shell, seed is taken out, note not clipping broken seed coat, some obvious empty grains or damaged seed should be rejected in time, and the seed that will shell is put into washbowl or bulk container, and glass bar fully stirs; After 30min, the seed that floats on the water surface is removed, be sunken to bottom seed and take out and drain moisture, this part seed is full seed; Full seed is placed on 3～5 layers of newspaper or blotting paper, and paper using blots the surface of the seed moisture;
(2) concentrated sulfuric acid acid etching seed: 100～150g seed is placed in to 1000ml beaker, adds the concentrated sulfuric acid of 250～300ml; Concentrated sulfuric acid etching time is 40～50min, and during this time, glass bar continues slowly to stir; The concentrated sulfuric acid in beaker is slowly inclined and, and the seed after acid etching uses running water fully to clean, and flowing water rinses 24h;
(3) magnetized drinking water is processed seed: in seed breaking dormancy and germination process, all use magnetized drinking water to process seed;
Affiliated magnetized drinking water is processed seed: 1) Gibberellins solution is used the preparation of 10000～15000GS magnetized drinking water; 2) in lamination process, moisture is provided by 10000～15000GS magnetized drinking water; 3) in germination process, moisture is provided by 10000～15000GS magnetized drinking water;
(4) Gibberellins solution seed soaking: the seed 48～60h that uses the Gibberellins solution of 1000～1500mg/L to soak seed after acid etching;
(5) cold stratification: the seed after seed soaking is placed in to wet rough sand, and planting husky volume ratio is 1: 3, puts into 0～5 DEG C of environment and carries out cold stratification; Rough sand adds magnetized drinking water, and addition is advisable with " hold agglomerating, unclamp loose ", in lamination process, stirs seed once, and according to circumstances suitably add magnetized drinking water every 5 days, and lamination is after 50～60 days, and seed is that breaking dormancy enters germinating;
(6) heat up and germinate: the seed that enters germinating is transferred in 23 ± 2 DEG C of illumination boxs by low temperature environment, intensity of illumination 10000～15000LX, light application time 10～12h/ days, the next day of duration of germination, observe sprouting situation, add in time magnetized drinking water, until after cotyledon launches completely, carry out bud transplantation of seedlings;
With this processing method, seed germination rate can reach 90% level, transplants planting percent and exceedes 85%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105284233B (en) * | 2015-02-04 | 2017-12-29 | 王振月 | A kind of Northeastern crowberry seed accelerating germination method |
| CN108834473A (en) * | 2018-07-18 | 2018-11-20 | 南京林业大学 | A method of releasing seed dormancy |
| CN110583146B (en) * | 2019-10-14 | 2024-01-19 | 安徽格瑞恩园林工程有限公司 | Treatment method for improving germination rate of Tilia miqueliana seeds |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101120634A (en) * | 2007-09-11 | 2008-02-13 | 南京林业大学 | Method for rapid relieving dormant state of nanjing linden seed |
| CN102599026A (en) * | 2012-03-30 | 2012-07-25 | 常熟市常福有机复合肥有限公司 | Tilia miqueliana seedling raising method |
| CN102668851A (en) * | 2012-05-29 | 2012-09-19 | 承德市神洲药业开发有限公司 | Linden cultivation method |
| RU2492625C2 (en) * | 2011-10-27 | 2013-09-20 | Государственное научное учреждение Поволжский научно-исследовательский институт производства и переработки мясомолочной продукции Российской академии сельскохозяйственных наук | Method of stimulation of seeds germination of agricultural crops |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101120634A (en) * | 2007-09-11 | 2008-02-13 | 南京林业大学 | Method for rapid relieving dormant state of nanjing linden seed |
| RU2492625C2 (en) * | 2011-10-27 | 2013-09-20 | Государственное научное учреждение Поволжский научно-исследовательский институт производства и переработки мясомолочной продукции Российской академии сельскохозяйственных наук | Method of stimulation of seeds germination of agricultural crops |
| CN102599026A (en) * | 2012-03-30 | 2012-07-25 | 常熟市常福有机复合肥有限公司 | Tilia miqueliana seedling raising method |
| CN102668851A (en) * | 2012-05-29 | 2012-09-19 | 承德市神洲药业开发有限公司 | Linden cultivation method |
Non-Patent Citations (3)
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| 椴树属植物研究进展;史锋厚等;《林业科技开发》;20061231;第20卷(第1期);12-15 * |
| 磁化浸种促进林木种子发芽生长的初步研究;程昌明等;《四川林勘设计》;20021231(第4期);16-18 * |
| 紫椴种子快速催芽育苗试验研究;罗丽芬等;《东北林业大学学报》;19900331;第18卷(第2期);103-108 * |
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