CN105265059A - Method for promoting seed germination of lithocarpus oleifolius A. camus - Google Patents
Method for promoting seed germination of lithocarpus oleifolius A. camus Download PDFInfo
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- CN105265059A CN105265059A CN201510739397.0A CN201510739397A CN105265059A CN 105265059 A CN105265059 A CN 105265059A CN 201510739397 A CN201510739397 A CN 201510739397A CN 105265059 A CN105265059 A CN 105265059A
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Abstract
The invention discloses a method for promoting the seed germination of lithocarpus oleifolius A. camus. The method is characterized by comprising the following steps of collecting seeds, pre-treating the seeds, storing the seeds in sand to promote sprouting, breeding, and managing and protecting the seeds. The mature lithocarpus oleifolius A. camus seeds are collected, and after being selected, sterilized, soaked and treated with gibberellin, the seeds are stored in the sand to promote sprouting, and the sprouting seeds are obtained by breeding, and managing and protecting the seeds. According to the method for promoting the seed germination of the lithocarpus oleifolius A. camus, seed dormancy can be effectively broken only by soaking the seeds in a plant hormone of proper concentration and storing the seeds in the sand, the seed germination is quickened, the germination rate is improved, the process operation is simple, the cost is low, and the method is convenient to operate and suitable for popularization, and has better economic benefit, social benefit and ecological benefit.
Description
Technical field
The present invention relates to the germination accelerating method of seed, specifically a kind of method promoting olive leaf Ke seed germination.
Background technology
Lan Yeke (
lithocarpusoleaefoliusa.Camus) olive leaf lithocarpus glaber, olive leaf C.-glauca is had another name called, it is Fagaceae (Fagaceae) Lithocarpus (Lithocarpus) arbor, high 8 ~ 15 meters, perula is several without hair, annual branch, petiole, blade back and rhachis are by caducous brown rust or the long pubescence of brown color, the dun black of biennial branch, hole skin is very little, not easily examines and sees.Bark dull gray, to grey black, does not ftracture, and thick about 8 millimeters of the bark that the height of tree is about 13 meters, endothelium reddish tan, ridge rib is significantly outstanding.Fruit is starch-containing is 50% ~ 60%.Be distributed in Jiangxi, Fujian, Hunan, Guizhou Si Sheng south, Guangdong, Guangxi.Be born in height above sea level 500 ~ 1200 meters of mountain region shaws.
At present, olive leaf Ke resource is still in wild distribution, but along with keeping the demand of Plant Diversity, large-scale artificial cultivation olive leaf Ke resource is imperative.Because olive leaf Ke seed belongs to hardseed, have several factors to cause seed germination success rate low in natural germination process, the reduction of resource is not supported in the sprouting naturally of seed far away.And the olive leaf Ke percentage of seedgermination without any hormone agents process in traditional mode of production often only has about 50%, and germinating time evening, emerge irregular, limit the large-scale planting of Lan Yeke.
Summary of the invention
Object of the present invention is the deficiency overcoming existing olive leaf Ke Seed germination techniques, provides a kind of germination rate and the quickening germination physiology that can improve olive leaf Ke seed, workable, practical simplicity, the method for promotion olive leaf Ke seed germination that cost is low.
Technical scheme of the present invention is as follows:
A kind of method promoting olive leaf Ke seed germination, comprise the operation that seed collection, Seeds preprocess, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe olive leaf Ke seed, carry out choosing seeds, sterilize, soak seed and implement after gibberellin process sand and hide vernalization, managed and protected the seed obtaining germinateing by breeding, main operational steps is as follows:
(1) seed collection: autumn gathers ripe olive leaf Ke seed, is placed in airtight container 2 ~ 3 days;
(2) Seeds preprocess:
1. choose seeds: by the olive leaf Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small;
2. sterilize: the liquor potassic permanganate clean olive leaf Ke seed being put into volumetric concentration 0.5% soaks 20 ~ 30min, then pulls out, dries in the shade after rinsing well with clear water;
3. soak seed: the clear water of the olive leaf Ke seed after sterilization is soaked 2 ~ 3d, makes its abundant imbibition; In clear water, fully stir olive leaf Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, olive leaf Ke seed is pulled out and drain;
4. gibberellin process: the Gibberellins solution olive leaf Ke seed after seed soaking being put into 400 ~ 600mg/L soaks 2 ~ 3d;
(3) husky Tibetan vernalization: the baking oven first river sand cleaned up being placed in 100 ~ 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, olive leaf Ke seed is shared equally on sand, flowerpot is placed in nursery, covers clean fine sand thin layer, and use covered rearing with plastic film moisturizing;
(4) breeding is managed and protected: to flowerpot periodically sprinkle bactericide during seed sand Tibetan, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity.
Preferably, the olive leaf Ke seed in above step (3) and sand are share equally after the ratio of 1:4 is puddled by volume.
Preferably, the even stand thickness described in above step (3) is no more than 20cm; The thickness of the clean fine sand of described covering is 2 ~ 3cm.
Preferably, the periodically sprinkle bactericide described in above step (4) be with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 8 ~ 10d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d; Described suitable humidity is that agglomerating, the humidity of scattering of loosing one's grip held by sand.
The present invention relative to prior art, the advantage had and good effect as follows:
1, the method for promotion olive leaf Ke seed germination of the present invention, do not need to divide the not easy-operating modes such as cotyledon through mechanical damage removal base portion seed coat, removal unit, only need soak and Sand culture by the Gibberellins solution of suitable concentration, just effectively seed dormancy can be broken, accelerate seed germination and improve germination rate, sprout nearly 30 days in advance than the olive leaf Ke seed without any hormone agents process in traditional mode of production, germination rate improves more than 30%.
2, the method for promotion olive leaf Ke seed germination of the present invention, olive leaf Ke seed is through gibberellin process and bury vernalization with fine sand, and sprout and shorten perdurabgility, germination peak period Relatively centralized, bud seedling high-quality, whole structure is good.
3, the present invention is placed in nursery by being equipped with the husky flowerpot hiding seed, and with covered rearing with plastic film, and moisturizing is carried out in timing watering, allows seed sprout in its natural state, is applicable to producing.
4, the present invention's potassium permanganate carries out disinfection to seed, and with the mode process sand of high-temperature sterilization, effectively prevents olive leaf Ke seed to produce and go mouldy, improve the germination rate of olive leaf Ke seed
5, the method for promotion olive leaf Ke seed germination of the present invention, technological operation is simple, with low cost, handled easily, is applicable to promoting, and has good economic benefit, social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1:
Autumn gathers ripe olive leaf Ke seed, is placed in airtight container 2 days.By the olive leaf Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean olive leaf Ke seed puts into volumetric concentration 0.5% is soaked 20min, then pulls out, dry in the shade after rinsing well with clear water.Olive leaf Ke seed clear water after sterilization is soaked 2d, makes its abundant imbibition; In clear water, fully stir olive leaf Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, olive leaf Ke seed is pulled out and drain.The Gibberellins solution that olive leaf Ke seed after seed soaking puts into 400mg/L is soaked 3d.
The baking oven first river sand cleaned up being placed in 100 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:5 by volume by olive leaf Ke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 20cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 8d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.
Embodiment 2:
Autumn gathers ripe olive leaf Ke seed, is placed in airtight container 2 days.By the olive leaf Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean olive leaf Ke seed puts into volumetric concentration 0.5% is soaked 25min, then pulls out, dry in the shade after rinsing well with clear water.Olive leaf Ke seed clear water after sterilization is soaked 2d, makes its abundant imbibition; In clear water, fully stir olive leaf Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, olive leaf Ke seed is pulled out and drain.The Gibberellins solution that olive leaf Ke seed after seed soaking puts into 500mg/L is soaked 2d.
The baking oven first river sand cleaned up being placed in 120 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:5 by volume by olive leaf Ke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 20cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 9d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.
Embodiment 3:
Autumn gathers ripe olive leaf Ke seed, is placed in airtight container 3 days.By the olive leaf Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The liquor potassic permanganate that clean olive leaf Ke seed puts into volumetric concentration 0.5% is soaked 30min, then pulls out, dry in the shade after rinsing well with clear water.Olive leaf Ke seed clear water after sterilization is soaked 3d, makes its abundant imbibition; In clear water, fully stir olive leaf Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, olive leaf Ke seed is pulled out and drain.The Gibberellins solution that olive leaf Ke seed after seed soaking puts into 600mg/L is soaked 2d.
The baking oven first river sand cleaned up being placed in 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, the ratio being 1:5 by volume by olive leaf Ke seed and sand is shared equally on sand after puddling, and even stand thickness is no more than 20cm.Flowerpot is placed in nursery, and cladding thickness is the clean fine sand thin layer of 2 ~ 3cm, and uses covered rearing with plastic film moisturizing.Seed sand hide during, with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 10d spray once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand holding agglomerating, the humidity of scattering of loosing one's grip.
Claims (4)
1. one kind promotes the method for olive leaf Ke seed germination, it is characterized in that: comprise the operation that seed collection, Seeds preprocess, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe olive leaf Ke seed, carry out choosing seeds, sterilize, soak seed and implement after gibberellin process sand and hide vernalization, managed and protected the seed obtaining germinateing by breeding, main operational steps is as follows:
(1) seed collection: autumn gathers ripe olive leaf Ke seed, is placed in airtight container 2 ~ 3 days;
(2) Seeds preprocess:
1. choose seeds: by the olive leaf Ke seed clean water after encapsulation process, then spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small;
2. sterilize: the liquor potassic permanganate clean olive leaf Ke seed being put into volumetric concentration 0.5% soaks 20 ~ 30min, then pulls out, dries in the shade after rinsing well with clear water;
3. soak seed: the clear water of the olive leaf Ke seed after sterilization is soaked 2 ~ 3d, makes its abundant imbibition; In clear water, fully stir olive leaf Ke seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, olive leaf Ke seed is pulled out and drain;
4. gibberellin process: the Gibberellins solution olive leaf Ke seed after seed soaking being put into 400 ~ 600mg/L soaks 2 ~ 3d;
(3) husky Tibetan vernalization: the baking oven first river sand cleaned up being placed in 100 ~ 140 DEG C is dried, naturally cool to room temperature, then sand is loaded in flowerpot, with the moistening sand of clear water, olive leaf Ke seed is shared equally on sand, flowerpot is placed in nursery, covers clean fine sand thin layer, and use covered rearing with plastic film moisturizing;
(4) breeding is managed and protected: to flowerpot periodically sprinkle bactericide during seed sand Tibetan, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity.
2. the method for promotion olive leaf Ke seed germination according to claim 1, is characterized in that: the olive leaf Ke seed in step (3) and sand are share equally after the ratio of 1:4 is puddled by volume.
3. the method for promotion olive leaf Ke seed germination according to claim 1, is characterized in that: the even stand thickness described in step (3) is no more than 20cm; The thickness of the clean fine sand of described covering is 2 ~ 3cm.
4. the method for promotion olive leaf Ke seed germination according to claim 1, is characterized in that: the periodically sprinkle bactericide described in step (4) be with volumetric concentration be 1 ‰ Baijunling solution to sterilization in the flowerpot of breeding bed, every 8 ~ 10d sprinkling once; Every day to spray water in the flowerpot of breeding bed once, sprays once husky bed every 10d; Described suitable humidity is that agglomerating, the humidity of scattering of loosing one's grip held by sand.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258457A (en) * | 2016-10-17 | 2017-01-04 | 柳州三农科技有限公司 | Implant acquisition methods outside a kind of Ke Lan Ye tissue culture |
| CN106258063A (en) * | 2016-07-29 | 2017-01-04 | 柳州创宇科技有限公司 | A kind of processing method improving seed of bitter gourd germination rate |
| CN106359106A (en) * | 2016-10-17 | 2017-02-01 | 柳州玲通科技有限责任公司 | Lithocarpus oleaefolius tissue culture seedling rooting inducing method |
| CN109005744A (en) * | 2018-09-15 | 2018-12-18 | 烟台市林业科学研究所 | A method of Callicarpa bodinieri Levl. seed germination rate is improved using gibberellin |
| CN109832179A (en) * | 2017-11-24 | 2019-06-04 | 黄钱英 | A kind of Chinese herbaceous peony soilless cultivation method for culturing seedlings |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103988607A (en) * | 2014-05-23 | 2014-08-20 | 广西壮族自治区林业科学研究院 | Method for promoting germination of lithocarpus polystachyus rehd seeds |
| CN104081980A (en) * | 2014-07-03 | 2014-10-08 | 广西壮族自治区林业科学研究院 | Method for lithocarpus polysachyus rehd container seedling culturing |
-
2015
- 2015-11-04 CN CN201510739397.0A patent/CN105265059A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103988607A (en) * | 2014-05-23 | 2014-08-20 | 广西壮族自治区林业科学研究院 | Method for promoting germination of lithocarpus polystachyus rehd seeds |
| CN104081980A (en) * | 2014-07-03 | 2014-10-08 | 广西壮族自治区林业科学研究院 | Method for lithocarpus polysachyus rehd container seedling culturing |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258063A (en) * | 2016-07-29 | 2017-01-04 | 柳州创宇科技有限公司 | A kind of processing method improving seed of bitter gourd germination rate |
| CN106258457A (en) * | 2016-10-17 | 2017-01-04 | 柳州三农科技有限公司 | Implant acquisition methods outside a kind of Ke Lan Ye tissue culture |
| CN106359106A (en) * | 2016-10-17 | 2017-02-01 | 柳州玲通科技有限责任公司 | Lithocarpus oleaefolius tissue culture seedling rooting inducing method |
| CN109832179A (en) * | 2017-11-24 | 2019-06-04 | 黄钱英 | A kind of Chinese herbaceous peony soilless cultivation method for culturing seedlings |
| CN109005744A (en) * | 2018-09-15 | 2018-12-18 | 烟台市林业科学研究所 | A method of Callicarpa bodinieri Levl. seed germination rate is improved using gibberellin |
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Application publication date: 20160127 |