CN103988607B - A kind of method promoting Pasania cuspidata seed germination - Google Patents
A kind of method promoting Pasania cuspidata seed germination Download PDFInfo
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- CN103988607B CN103988607B CN201410220346.2A CN201410220346A CN103988607B CN 103988607 B CN103988607 B CN 103988607B CN 201410220346 A CN201410220346 A CN 201410220346A CN 103988607 B CN103988607 B CN 103988607B
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Abstract
The invention discloses a kind of method promoting Pasania cuspidata seed germination, comprise the operation that seed collection, Seeds preprocess, the preparation of husky bed, husky Tibetan vernalization and breeding are managed and protected, 10 ~ November by gathering ripe Pasania cuspidata seed, carry out choosing seeds, to wash kind, implement after sterilization and Gibberellins solution seed soaking huskyly to hide vernalization, managed and protected the Pasania cuspidata seed obtaining germinateing by breeding.Present invention uses the resting stage that Gibberellins solution breaks Pasania cuspidata seed, obviously shorten the germination period of seed, improve the germination rate of Pasania cuspidata seed, Pasania cuspidata seed sprouts nearly 20 days in advance, and germination rate improves more than 20%.Present invention process is simple to operate, and cost is low, Pasania cuspidata seed can be impelled to sprout fast, neatly, efficiently, have good economic benefit, social benefit and ecological benefits.
Description
Technical field
The present invention relates to the germination accelerating method of seed, specifically a kind of method promoting Pasania cuspidata seed germination.
Background technology
Pasania cuspidata (
lithocarpuspolystachyusRehd) have another name called sweet tea, being Fagaceae (Fagaceae) Lithocarpus (Lithocarpus) aiphyllium, is China's Traditional Folk herbal medicine and health drink plant.Its leaf contains sweet theine, is Gao Tian, low-heat, avirulent natural sweetener.Research in recent years confirms that Pasania cuspidata leaf is also rich in 17 seed amino acids, various trace elements, the vitamin of needed by human, phloridzin, trifloroside and multiple flavonoids and the chemical composition such as polyphenol, triterpene class, have and remove human free radical, anti-ageing, antiallergy, hemangiectasis, regulate vascular permeability, prevent arteriosclerosis, hypotensive, lipopenicillinase, diuresis, anti-inflammation, the pharmacological action such as inhibition tumor cell.All certain effect is had for prevention of obesity disease, diabetes, cardiovascular disease, high blood pressure, arteriosclerosis, carious tooth etc.
At present, Pasania cuspidata resource is still in wild distribution, but along with growing Chinese medicine consumption demand, large-scale artificial cultivation Pasania cuspidata resource is imperative.Because Pasania cuspidata seed belongs to hardseed, have several factors to cause seed germination success rate low in natural germination process, the reduction of resource is not supported in the sprouting naturally of seed far away.And often only have about 50% without the Pasania cuspidata percentage of seedgermination of any hormone agents process in traditional mode of production, and germinating time evening, emerge irregular, limit the large-scale production of Pasania cuspidata seedling.
Summary of the invention
Object of the present invention is the deficiency overcoming existing Pasania cuspidata Seed germination techniques, provides a kind of germination rate and the quickening germination physiology that can improve Pasania cuspidata seed, workable, practical simplicity, the method for the promotion Pasania cuspidata seed germination that cost is low.
The present invention is achieved by the following technical solutions:
A kind of method promoting Pasania cuspidata seed germination, comprise the operation that seed collection, Seeds preprocess, the preparation of husky bed, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe Pasania cuspidata seed, carry out choosing seeds, to wash kind, implement after sterilization and seed soaking huskyly to hide vernalization, managed and protected the seed obtaining germinateing by breeding, main operational steps is as follows:
(1) seed collection: 10 ~ November, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard bright time implement collect seed, obtain fresh Pasania cuspidata seed;
(2) Seeds preprocess:
1. choose seeds: the fresh Pasania cuspidata seed collected is spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small;
2. kind is washed: the Pasania cuspidata seed clear water chosen is soaked 40 ~ 50h, makes its abundant imbibition; In clear water, fully stir Pasania cuspidata seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drain;
3. sterilize: putting into cleaning the Pasania cuspidata seed after draining liquor potassic permanganate immersion 50 ~ 70min that volumetric concentration is 0.5%, then pulling out, drying in the shade after rinsing well with clear water;
4. soak seed: the Gibberellins solution Pasania cuspidata seed after sterilization being put into 100 ~ 600mg/L soaks 40 ~ 50h;
(3) husky bed prepares: in husky bed, first spread the thin river sand of the thick fresh clean of 8 ~ 12cm, sowing the previous day to thin river sand fully spray volumetric concentration be 0.5 ~ 1% liquor potassic permanganate carry out disinfection;
(4) sand hides vernalization: the ratio being 1:4 by volume by the Pasania cuspidata seed after seed soaking and sand is puddled, evenly be spread out on husky bed, then cover clean thin river sand again, water of then fully drenching, finally cover husky bed surface carry out moisturizing with being clear to translucent clean White plastic film;
(5) breeding is managed and protected: to husky bed periodically sprinkle bactericide during seed sand Tibetan, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity.
Preferably, the husky bed described in above step (3) is selected at and is leewardly built into fragment of brick on the sunny side, on well-drained level land, and the height of husky bed is 30 ~ 40cm.
Preferably, the described even stand thickness in above step (4) is no more than 20cm; The thickness of the clean thin river sand of described covering is 4 ~ 5cm.
Preferably, the periodically sprinkle bactericide described in above step (5) is 1 ‰ carbendazim solutions by volumetric concentration, sprays once husky bed every 12 ~ 18d; Described suitable humidity is that agglomerating, the humidity of scattering of loosing one's grip held by sand.
The present invention relative to prior art, the advantage had and good effect as follows:
1, the method for promotion Pasania cuspidata seed germination of the present invention, do not need to divide the not easy-operating modes such as cotyledon through mechanical damage removal base portion seed coat, removal unit, only need soak and Sand culture by the Gibberellins solution of suitable concentration, just effectively seed dormancy can be broken, accelerate seed germination and improve germination rate, sprout nearly 20 days than in traditional mode of production in advance without the Pasania cuspidata seed of any hormone agents process, germination rate improves more than 20%.
2, the method for promotion Pasania cuspidata seed germination of the present invention, Pasania cuspidata seed is through gibberellin process and bury vernalization with fine sand, and sprout and shorten perdurabgility, germination peak period Relatively centralized, bud seedling high-quality, whole structure is good.
3, the method for promotion Pasania cuspidata seed germination of the present invention, technological operation is simple, with low cost, handled easily, is applicable to promoting, and has good economic benefit, social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1:
In early October, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard bright time implement collect seed, obtain fresh Pasania cuspidata seed.The fresh Pasania cuspidata seed collected is spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The Pasania cuspidata seed clear water chosen is soaked 40h, makes its abundant imbibition; In clear water, fully stir Pasania cuspidata seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drain.By clean drain after Pasania cuspidata seed put into volumetric concentration be 0.5% liquor potassic permanganate soak 50min, then pull out, dry in the shade after rinsing well with clear water.The Gibberellins solution that Pasania cuspidata seed after sterilization puts into 100mg/L is soaked 40h and impels germination with the resting stage of breaking seed; Get husky bed in advance ready during seed soaking, husky bed is selected at and is leewardly built into fragment of brick on the sunny side, on well-drained level land, and the height of husky bed is 30cm.In husky bed, first spread the thin river sand of the thick fresh clean of 8cm, sowing the previous day to thin river sand fully spray volumetric concentration be 0.5% liquor potassic permanganate carry out disinfection.By the Pasania cuspidata seed after seed soaking and husky by volume for the ratio of 1:4 is puddled, be evenly spread out on husky bed, even stand thickness is no more than 20cm; Then cover clean thin river sand again, the thickness covering clean thin river sand is 4cm, water of then fully drenching, and finally covers husky bed surface carry out moisturizing with being clear to translucent clean White plastic film.It is 1 ‰ carbendazim solution bactericide that seed sand regularly sprays a volumetric concentration to husky bed every 12d during hiding, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity; Sand be suitable for humidity be sand holding agglomerating, the humidity of scattering of loosing one's grip, until seed sprouting, the germination rate of seed is 81.7%.
Embodiment 2:
Mid-October, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard bright time implement collect seed, obtain fresh Pasania cuspidata seed.The fresh Pasania cuspidata seed collected is spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The Pasania cuspidata seed clear water chosen is soaked 48h, makes its abundant imbibition; In clear water, fully stir Pasania cuspidata seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drain.By clean drain after Pasania cuspidata seed put into volumetric concentration be 0.5% liquor potassic permanganate soak 60min, then pull out, dry in the shade after rinsing well with clear water.The Gibberellins solution that Pasania cuspidata seed after sterilization puts into 200mg/L is soaked 48h and impels germination with the resting stage of breaking seed; Get husky bed in advance ready during seed soaking, husky bed is selected at and is leewardly built into fragment of brick on the sunny side, on well-drained level land, and the height of husky bed is 35cm.In husky bed, first spread the thin river sand of the thick fresh clean of 9cm, sowing the previous day to thin river sand fully spray volumetric concentration be 1% liquor potassic permanganate carry out disinfection.By the Pasania cuspidata seed after seed soaking and husky by volume for the ratio of 1:4 is puddled, be evenly spread out on husky bed, even stand thickness 10cm; Then cover clean thin river sand again, the thickness covering clean thin river sand is 4cm, water of then fully drenching, and finally covers husky bed surface carry out moisturizing with being clear to translucent clean White plastic film.It is 1 ‰ carbendazim solution bactericide that seed sand regularly sprays a volumetric concentration to husky bed every 14d during hiding, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity; Sand be suitable for humidity be sand holding agglomerating, the humidity of scattering of loosing one's grip, until seed sprouting, the germination rate of seed is 84.7%.
Embodiment 3:
In early November, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard bright time implement collect seed, obtain fresh Pasania cuspidata seed.The fresh Pasania cuspidata seed collected is spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The Pasania cuspidata seed clear water chosen is soaked 48h, makes its abundant imbibition; In clear water, fully stir Pasania cuspidata seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drain.By clean drain after Pasania cuspidata seed put into volumetric concentration be 0.5% liquor potassic permanganate soak 60min, then pull out, dry in the shade after rinsing well with clear water.The Gibberellins solution that Pasania cuspidata seed after sterilization puts into 400mg/L is soaked 48h and impels germination with the resting stage of breaking seed; Get husky bed in advance ready during seed soaking, husky bed is selected at and is leewardly built into fragment of brick on the sunny side, on well-drained level land, and the height of husky bed is 35cm.In husky bed, first spread the thin river sand of the thick fresh clean of 10cm, sowing the previous day to thin river sand fully spray volumetric concentration be 1% liquor potassic permanganate carry out disinfection.By the Pasania cuspidata seed after seed soaking and husky by volume for the ratio of 1:4 is puddled, be evenly spread out on husky bed, even stand thickness is 15cm; Then cover clean thin river sand again, the thickness covering clean thin river sand is 5cm, water of then fully drenching, and finally covers husky bed surface carry out moisturizing with being clear to translucent clean White plastic film.It is 1 ‰ carbendazim solution bactericide that seed sand regularly sprays a volumetric concentration to husky bed every 15d during hiding, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity; Sand be suitable for humidity be sand holding agglomerating, the humidity of scattering of loosing one's grip, until seed sprouting, the germination rate of seed is 76.7%.
Embodiment 4:
In late October, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard bright time implement collect seed, obtain fresh Pasania cuspidata seed.The fresh Pasania cuspidata seed collected is spread ventilating, without the indoor of direct sunlight, the impurity such as artificial removal's acorn-cup, sprig, blade, and removing have worm channel, form not just, have and damage and seed that form is too small.The Pasania cuspidata seed clear water chosen is soaked 50h, makes its abundant imbibition; In clear water, fully stir Pasania cuspidata seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drain.By clean drain after Pasania cuspidata seed put into volumetric concentration be 0.5% liquor potassic permanganate soak 70min, then pull out, dry in the shade after rinsing well with clear water.The Gibberellins solution that Pasania cuspidata seed after sterilization puts into 600mg/L is soaked 50h and impels germination with the resting stage of breaking seed; Get husky bed in advance ready during seed soaking, husky bed is selected at and is leewardly built into fragment of brick on the sunny side, on well-drained level land, and the height of husky bed is 40cm.In husky bed, first spread the thin river sand of the thick fresh clean of 12cm, sowing the previous day to thin river sand fully spray volumetric concentration be 1% liquor potassic permanganate carry out disinfection.By the Pasania cuspidata seed after seed soaking and husky by volume for the ratio of 1:4 is puddled, be evenly spread out on husky bed, even stand thickness is 20cm; Then cover clean thin river sand again, the thickness covering clean thin river sand is 5cm, water of then fully drenching, and finally covers husky bed surface carry out moisturizing with being clear to translucent clean White plastic film.It is 1 ‰ carbendazim solution bactericide that seed sand regularly sprays a volumetric concentration to husky bed every 18d during hiding, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity; Sand be suitable for humidity be sand holding agglomerating, the humidity of scattering of loosing one's grip, until seed sprouting, the germination rate of seed is 78.3%.
Claims (3)
1. one kind promotes the method for Pasania cuspidata seed germination, it is characterized in that: comprise the operation that seed collection, Seeds preprocess, the preparation of husky bed, husky Tibetan vernalization and breeding are managed and protected, by gathering ripe Pasania cuspidata seed, carry out choosing seeds, to wash kind, implement after sterilization and seed soaking huskyly to hide vernalization, managed and protected the seed obtaining germinateing by breeding, main operational steps is as follows:
(1) seed collection: 10 ~ November, when the kind shell color of Pasania cuspidata seed becomes yellowish-brown or Chestnut from green, hard bright time implement collect seed, obtain fresh Pasania cuspidata seed;
(2) Seeds preprocess:
1. choose seeds: the fresh Pasania cuspidata seed collected is spread ventilating, without the indoor of direct sunlight, artificial removal's acorn-cup, sprig, blade impurity, and removing have worm channel, form not just, have and damage and seed that form is too small;
2. kind is washed: the Pasania cuspidata seed clear water chosen is soaked 40 ~ 50h, makes its abundant imbibition; In clear water, fully stir Pasania cuspidata seed again, then leave standstill, remove the floater or ghost seed that swim on the water surface, finally repeatedly wash remaining seed by rubbing with the hands, after cleaning, Pasania cuspidata seed is pulled out and drain;
3. sterilize: putting into cleaning the Pasania cuspidata seed after draining liquor potassic permanganate immersion 50 ~ 70min that volumetric concentration is 0.5%, then pulling out, drying in the shade after rinsing well with clear water;
4. soak seed: the Gibberellins solution Pasania cuspidata seed after sterilization being put into 100 ~ 600mg/L soaks 40 ~ 50h;
(3) husky bed prepares: in husky bed, first spread the thin river sand of the thick fresh clean of 8 ~ 12cm, sowing the previous day to thin river sand fully spray volumetric concentration be 0.5 ~ 1% liquor potassic permanganate carry out disinfection;
(4) sand hides vernalization: the ratio being 1:4 by volume by the Pasania cuspidata seed after seed soaking and sand is puddled, evenly be spread out on husky bed, then cover clean thin river sand again, water of then fully drenching, finally cover husky bed surface carry out moisturizing with being clear to translucent clean White plastic film; Described even stand thickness is no more than 20cm; The thickness of the clean thin river sand of described covering is 4 ~ 5cm;
(5) breeding is managed and protected: to husky bed periodically sprinkle bactericide during seed sand Tibetan, and according to moisture evaporation situation in sand, sprays a small amount of clear water in good time, keep sand to be suitable for humidity.
2. a kind of method promoting Pasania cuspidata seed germination according to claim 1, is characterized in that: the husky bed described in step (3) is selected at and is leewardly built into fragment of brick on the sunny side, on well-drained level land, and the height of husky bed is 30 ~ 40cm.
3. a kind of method promoting Pasania cuspidata seed germination according to claim 1, is characterized in that: the periodically sprinkle bactericide described in step (5) is 1 ‰ carbendazim solutions by volumetric concentration, sprays once husky bed every 12 ~ 18d; Described suitable humidity is that agglomerating, the humidity of scattering of loosing one's grip held by sand.
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| CN105325097A (en) * | 2015-11-04 | 2016-02-17 | 张莘蔓 | Method for promoting seed germination of lithocarpus calophyllus |
| CN105265062A (en) * | 2015-11-04 | 2016-01-27 | 张莘蔓 | Method for promoting seed germination of lithocarpus elmerrillii |
| CN105265059A (en) * | 2015-11-04 | 2016-01-27 | 张莘蔓 | Method for promoting seed germination of lithocarpus oleifolius A. camus |
| CN105265058A (en) * | 2015-11-04 | 2016-01-27 | 张莘蔓 | Method for improving germination rate of Lithocarpus craibianus seeds |
| CN105325096A (en) * | 2015-11-04 | 2016-02-17 | 张莘蔓 | Method for promoting germination of lithocarpus amygdalifolius seeds |
| CN105284236A (en) * | 2015-11-04 | 2016-02-03 | 张莘蔓 | Method for promoting germination of quercus acutissima seeds |
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| CN107231911A (en) * | 2017-05-24 | 2017-10-10 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of method for improving extremely frigid zones grass vetch yield |
| CN107306533A (en) * | 2017-06-19 | 2017-11-03 | 四川森迪科技发展股份有限公司 | A kind of Idesia polycarpa method for treating seeds of high bud ratio |
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| CN102487679A (en) * | 2011-11-17 | 2012-06-13 | 赵声驰 | Cutting propagation method for lithocarpus polystachyus rehd |
| CN103004323A (en) * | 2013-01-16 | 2013-04-03 | 广西壮族自治区林业科学研究院 | Method for promoting germination of cinnamon seed |
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