CN105917792B - A method of improving paris polyphylla seeding ratio - Google Patents
A method of improving paris polyphylla seeding ratio Download PDFInfo
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- CN105917792B CN105917792B CN201610363884.6A CN201610363884A CN105917792B CN 105917792 B CN105917792 B CN 105917792B CN 201610363884 A CN201610363884 A CN 201610363884A CN 105917792 B CN105917792 B CN 105917792B
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- paris polyphylla
- exosper
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The present invention discloses a kind of method improving paris polyphylla seeding ratio, is related to seed biology techniques field.This method be characterized in by acquisition ripe paris polyphylla fruit place to exosper rot after rub exosper with the hands again, seed impregnates 30 minutes, a concentration of 1000mg/L of its gibberellin with 75% alcohol of dissolving gibberellin, it is impregnated 10 hours with 40~45 DEG C of water again, seed is then subjected to alternating temperature Sand culture round the clock, seedbed is sown into after budding, blinding covers pine needle, takes sunshade net.This method makes paris polyphylla seed germination rate reach 99% or more, and seeding ratio reaches 85% or more, and compared with existing comprehensive paris polyphylla seed sprouting method, seeding ratio improves 76%.
Description
Technical field
The present invention relates to seed biology techniques fields, and in particular to increases Paris polyphylla seed sprouting using different disposal condition
The method of rate.
Background technology
Paris polyphylla (Paris polyphylla var.yunnanensis) is Liliaceae Paris herbaceos perennial,
It is the medicinal Paris polyphylla original plant species that pharmacopeia is included.Its rhizome has the function of clearing heat and detoxicating, relieving convulsion analgesic, can be used for abscess of throat,
Child convulsion, venomous snake bite, furuncle swelling toxin, control furuncle outside, and parotitis is the Chinese patent drugs such as Yunnan Baiyao, Gong Xue Ning, piece son's Huang
Primary raw material.For a long time, people rely on always wild resource to the utilization of paris polyphylla, due to long-term excessive predation formula
Excavation, wild paris polyphylla has been on the verge of exhaustion by destructive destruction, and artificial growth becomes that solve paris polyphylla resource deficient
Weary inevitable choice.
Paris polyphylla seed has hibernation feature, such as allows it to sprout naturally, then needs could emerge within 15 months, and emergence rate is difficult to
Reach 50%.And cool stratification is used, the method for secondary alternating temperature, though can emerge in 1 year, emergence rate is only 60%.Its reason
It is paris polyphylla embryo differentiation not exclusively (form after-ripening) and plumular axis after-ripening (physiology after-ripening).
Form after-ripening refers to the ripe stage from seed maturity to embryo, and embryo slowly grows and shapes in seed.Yunnan weight
The seed harvested after building fruit maturation, as seed maturity.The embryo of mature seed is very immature, and at this moment the form composition of embryo is not still
It is perfect, it cannot germinate.Under epidemic disaster appropriate and air conditions, embryo starts differentiation and growth, differentiates cotyledon, plumule, embryo
Axis and radicle have been basically completed the construction of idiosome, this phase is form after-ripening.After embryo completion morphology after-ripening, suitable hair is given
The environmental condition of bud, seed cannot still be unearthed sprouting at once, the cryogenic conditions of certain time be needed, after plumular axis is by its physiology
Ripe, seed could be sprouted.Two stages of the after-ripening of seed completion morphology and physiology after-ripening of paris polyphylla under field conditions (factors), i.e., from fruit
The real ripe time that 15 months are needed to seed sprouting.
About the breeding of paris polyphylla, domestic scholars have done numerous studies, Li Yunchang (Li Yunchang, paris polyphylla it is asexual numerous
It grows, Yunnan plant research, 1986,8 (4):Numerous studies 429-435) are done to the seminal propagation of paris polyphylla, paris polyphylla seed is certainly
It so sprouts, cool canopy seedbed is directly sowed at without processing, 15 months emergence rates are 46.2%.Li Heng, Yang Xinghua pass through to Paris polyphylla
The Morphological And Anatomical observation that seed is sprouted：When Paris polyphylla seed shedding, embryonic pole agensis, but a very little, do not break up completely it is more
Cell ellipsoid, surrounding albuminous cell is thick and keratinization, inner face are filled with amylum body and other nutriments, make endosperm group
It knits and is hardened, and be unfavorable for the entrance of air and moisture, the nutriment in seed cannot also soften the dissolving substance easily to absorb
Nutrition when as embryonic development, therefore Paris polyphylla seed has latter stage of ripening for the differentiation for completing embryo.Li Yunchang analyzes paris polyphylla
The hibernation feature of seed, after low temperature, moist preliminary treatment, although high-temperature cultivation, radicle continues to increase, rhizome, plumular axis
Developed, but seed cannot still break ground emergence.It is primarily due to the slow-growing of plumular axis, there are physiology after ripenings for plumular axis, then
Through low-temperature treatment, then high-temperature cultivation, the growth of plumular axis can be effectively facilitated, seed breaks ground emergence soon, seed sprout time is made to shorten
It is 10~12 months., studies have shown that cool stratification can be such that paris polyphylla embryo preferably develops, temperature is to influence paris polyphylla seed layer for this
An important factor for product effect.In terms of application exogeneous growth conditioning agent promotes paris polyphylla seed afterripening, majority research still thinks suitable
When the processing of the gibberellin of concentration is conducive to the germination rate of Paris polyphylla seed.
Although many on how to the research for improving paris polyphylla seed germination rate, researcher mostly concentrates on sight single
Influence of the change external condition to Paris polyphylla seed germination rate on, and germination efficiency is not very ideal, and in terms of emergence rate
Researches.
The present inventor inhibits the mechanism that paris polyphylla seed is sprouted by comprehensive analysis, in conjunction with the characteristics of seed maturity, invention
A kind of method that can substantially increase paris polyphylla seeding ratio.Pass through update search twice in 2013 to 2015, mesh
The preceding relevant report at home and abroad finding no such method.
Invention content
To solve the low technical problem of paris polyphylla seeding ratio, what the present invention provided a kind of other methods of difference can be significantly
Improve the new method of paris polyphylla seeding ratio (85% or more).
A kind of method improving paris polyphylla seeding ratio provided by the present invention, its step are as follows：
(1) the ripe paris polyphylla fruit of acquisition is placed to exosper and is rotted, then rub exosper with the hands；
(2) the seed 75%v/v alcohol of a concentration of 1000mg/L containing gibberellin for removing exosper will be rubbed with the hands and impregnates 30
Minute；
(3) 40~45 DEG C of water of step (2) treated seed are impregnated 10 hours；
(4) step (3) treated seed is 1 by the volume ratio of seed and wet sand by alternating temperature Sand culture:3 ratio
Example, seed is uniformly mixed with wet sand, puts into and is placed in growth cabinet in basin, and 30 DEG C 12h/25 DEG C is used under dark condition
After 12h intermittent warmings 40~50 days, seed is sown into seedbed, blinding covers pine needle, takes the sunshade net that shading rate is 80%, wet sand
Humid control is 68%~78%.
The present invention, will be unique on the basis of furtheing investigate the physiological property of paris polyphylla seed and summarizing former achievements
Other people the not found seeds that may advantageously facilitate sprout method with the method for emergence and traditional promotions paris polyphylla seed sprouting
It is organically integrated, paris polyphylla seeding ratio is made to reach 85% or more, compared with existing comprehensive seed germination method, seed
Emergence rate improves 76%, produces unexpected technique effect.
The innovative point of the method for the present invention is：
1, it rubs with the hands to go before exosper to place to rub with the hands after exosper rots and removes exosper, conventional method is the ripe Yunnan weight that will be acquired
Building fruit is directly rubbed with the hands and removes exosper, and alternating temperature stratification processing is carried out after then being impregnated with gibberellin；The method of the present invention goes outer kind in stranding
It places to rub with the hands after exosper rots before skin and removes exosper, it is therefore an objective to allow seed under conditions of thering is pericarp to supply nutrient, in fruit
It further reaches maturity, the method that exosper is directly removed after this step and aforementioned conventional acquisition seed in seed germination rate and goes out
Want much higher in terms of the raising of seedling rate, seeding ratio improves 31%.
2, measure is impregnated using the alcohol containing gibberellin, i.e., will rubs with the hands and goes the seed of exosper red mould with 1000mg/L is contained
Element 75% alcohol impregnate seed half an hour, after with warm water again invade bubble 10 hours, increase kind of a transdermally, be conducive to Exogenous gibberellic acid
Entrance.This measure makes seeding ratio improve 60%.
3, alternating temperature Sand culture is carried out, under dark condition, 30 DEG C of 12h/25 DEG C of 12h intermittent warmings are sown into after 40-50 days
Seedbed.This measure makes seed germination rate be increased to 99%, and the emergence rate in later stage reaches 85% or more.
The method of the present invention instrument is simple, easy to operate, is easy to carry out batch production Paris polyphylla seeling industry.
Specific implementation mode
Test material used in following embodiment is commercially available.
1 the method for the present invention of embodiment
Key instrument：Growth cabinet (RGX-250)
Steps are as follows：
(1) the ripe paris polyphylla fruit of acquisition is placed to exosper and is rotted, then rub exosper with the hands；
(2) the seed 75%v/v alcohol of a concentration of 1000mg/L containing gibberellin for removing exosper will be rubbed with the hands and impregnates 30
Minute；
(3) by step (2) after the 75%v/v alcohol of a concentration of 1000mg/L containing gibberellin impregnates processing in 30 minutes
Seed impregnated 10 hours with 45 DEG C of water；
(4) alternating temperature Sand culture, by step (3) 45 DEG C of water seed that impregnates that treated in 10 hours, by seed and wet sand
Volume ratio be 1:Seed is uniformly mixed with wet sand, puts into and be placed in growth cabinet in basin, under dark condition by 3 ratio
After daily 30 DEG C 12h/25 DEG C of 12h intermittent warmings 50 days, seed is sown into seedbed, blinding covers pine needle, takes shading rate and is
80% sunshade net, the humid control of the wet sand is 68%.Emergence rate is counted after seed sprouting.(the side of the present invention of embodiment 1
Method) so that paris polyphylla seed germination rate is reached 99% or more, paris polyphylla seeding ratio reaches 85% or more.
Embodiment 2- embodiments 4 are the comparative experiments by changing monofactor and embodiment 1.
Embodiment 2
Exosper monofactor is directly gone to be placed to outer kind with ripe paris polyphylla fruit by changing ripe paris polyphylla fruit
Skin rots to rub exosper with the hands again and compare its influence to paris polyphylla seeding ratio.
Embodiment 2：In addition to except the ripe paris polyphylla fruit for acquiring step (1), directly exosper is removed in stranding, remaining step and reality
It is identical to apply example 1.
The experimental results showed that (referring to table 1)：Embodiment 2：Ripe paris polyphylla fruit is directly rubbed with the hands and goes exosper, paris polyphylla kind
Sub- emergence rate is 67% ± 2%.
The method of the present invention improves 31.34% than embodiment 2, paris polyphylla seeding ratio, i.e., [(88-67)/76] ×
100%=31.34%.
Embodiment 3
By change with 1000mg/L gibberellin aqueous solution soaking monofactors with containing 1000mg/L gibberellin
Its influence to paris polyphylla seeding ratio is compared in the immersion of 75% alcohol.
Embodiment 3 is in addition to step (2) is to be impregnated 30 minutes with the water of 1000mg/L gibberellin, remaining step and embodiment 1
It is identical.
The experimental results showed that (referring to table 1)：The paris polyphylla seeding ratio of embodiment 3 is 58% ± 4%.
The method of the present invention improves 51% than embodiment 3, paris polyphylla seeding ratio, i.e., [(88-58)/58] ×
100%=51%.
Embodiment 4
By change seed sand storage during Temperature Treatment monofactor compared with embodiment 1 its to paris polyphylla seed sprouting
The influence of rate
It is that 4 DEG C of processing handle 60 for 60 days and 20 DEG C beyond the highest heavens that embodiment 4, which removes temperature in step (4) alternating temperature Sand culture, remaining
Step is same as Example 1.
The experimental results showed that (referring to table 1)：The paris polyphylla seeding ratio of embodiment 4 is 62% ± 5%.
The method of the present invention improves 41% than embodiment 4, paris polyphylla seeding ratio, i.e. [(88-62)/62=41%.
5 embodiment of embodiment, 1 the method for the present invention is compared with existing comprehensive paris polyphylla seed germination method
The existing comprehensive paris polyphylla seed sprouting method and step of embodiment 5 is as follows：
(1) it rubs with the hands and removes exosper, exosper is removed into the ripe paris polyphylla fruit stranding of acquisition；
(2) with the gibberellin aqueous solution soaking of a concentration of 1000mg/L 30 minutes；
(3) seed of the gibberellin aqueous solution soaking through 1000mg/L is pressed seed and the body of wet sand by alternating temperature Sand culture
Product is than being 1:Seed is uniformly mixed with wet sand, puts into and be placed in growth cabinet in basin, using intermittent warming mode by 3 ratio：
After 4 DEG C of processing processing in 60 days and 20 DEG C 60 days, seed is sown into seedbed, blinding covers pine needle, takes the sunshade that shading rate is 80%
Net, sand humid control 68%.Emergence rate is counted after seed sprouting.
Experiment effect is shown in Table 1：The existing comprehensive paris polyphylla seed sprouting method of embodiment 5, paris polyphylla seeding ratio are
50% ± 4%.
The method of the present invention improves 76% than embodiment 5, paris polyphylla seeding ratio, i.e. [(88-50)/50] × 100%
=76%.
Paris polyphylla seed germination rate comparable situation under 1 different disposal method of table
Claims (1)
1. a kind of method improving paris polyphylla seeding ratio, it is characterized in that：
(1) the ripe paris polyphylla fruit of acquisition is placed to exosper and is rotted, then rub exosper with the hands；
(2) the seed 75%v/v alcohol of a concentration of 1000mg/L containing gibberellin for removing exosper will be rubbed with the hands to impregnate 30 minutes；
(3) 40~45 DEG C of water of step (2) treated seed are impregnated 10 hours；
(4) step (3) treated seed is 1 by the volume ratio of seed and wet sand by alternating temperature Sand culture:3 ratio, will
Seed is uniformly mixed with wet sand, is put into and is placed in growth cabinet in basin, and 30 DEG C of 12h/25 DEG C of 12h alternating temperatures are used under dark condition
After processing 40~50 days, seed is sown into seedbed, blinding covers pine needle, takes the sunshade net that shading rate is 80%, wet sand humid control
68%~78%.
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107027624B (en) * | 2017-04-28 | 2019-07-23 | 云南农业大学 | A method of obtaining polygerm paris polyphylla seedling |
| CN108040530A (en) * | 2017-12-01 | 2018-05-18 | 亳州学院 | The wild Paris polyphylla seed dormancy in Dabie Mountain breaks method |
| CN111937525A (en) * | 2020-08-11 | 2020-11-17 | 湖南农佳好伙计农业科技发展有限公司 | Paris polyphylla seed sand storage, temperature change and medicament treatment germination accelerating method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222362A (en) * | 2013-04-27 | 2013-07-31 | 云南农业大学 | Method for improving paris polyphylla var. yunnanensis seed germination rate |
| CN104521515A (en) * | 2014-12-29 | 2015-04-22 | 兴安盟京蒙中药材科技开发有限公司 | Method for increasing bud ratio of radix paeoniae rubra seeds |
| CN105210647A (en) * | 2015-10-28 | 2016-01-06 | 云南祥仁生物科技有限公司 | The method for massively culturing of a kind of Yunnan Paris polyphylla seedling |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222362A (en) * | 2013-04-27 | 2013-07-31 | 云南农业大学 | Method for improving paris polyphylla var. yunnanensis seed germination rate |
| CN104521515A (en) * | 2014-12-29 | 2015-04-22 | 兴安盟京蒙中药材科技开发有限公司 | Method for increasing bud ratio of radix paeoniae rubra seeds |
| CN105210647A (en) * | 2015-10-28 | 2016-01-06 | 云南祥仁生物科技有限公司 | The method for massively culturing of a kind of Yunnan Paris polyphylla seedling |
Non-Patent Citations (2)
| Title |
|---|
| 变温层积对解除滇重楼种子休眠及其内源激素变化的研究;陈疏影 等;《中草药》;20110430;第42卷(第4期);793-795 * |
| 滇重楼种子无菌萌发及植株形态发生的研究;杨淋 等;《中国实验方剂学杂志》;20130228;第19卷(第3期);151-154 * |
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