CN105917792A - Method for increasing seedling emergence rate of paris polyphylla seeds - Google Patents
Method for increasing seedling emergence rate of paris polyphylla seeds Download PDFInfo
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- CN105917792A CN105917792A CN201610363884.6A CN201610363884A CN105917792A CN 105917792 A CN105917792 A CN 105917792A CN 201610363884 A CN201610363884 A CN 201610363884A CN 105917792 A CN105917792 A CN 105917792A
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- seed
- rhizoma paridis
- episperm
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- paris polyphylla
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a method for increasing seedling emergence rate of paris polyphylla seeds, and relates to the technical field of seed biology. The method is characterized by comprising the following steps: placing the collected mature paris polyphylla fruits until the episperm is rotted; rubbing to remove the episperm; soaking the seeds for 30 minutes through 75% of alcohol in which gibberellin is dissolved, wherein the concentration of the gibberellin is 1000mg/L; soaking for 10 hours in water at the temperature of 40 to 45 DEG C; treating the seeds in sand under diurnal changed temperature; transferring the seeds to a seedbed after budding; covering with soil; covering with pine needles; arranging a sunshade net. With the adoption of the method, the germination rate of the paris polyphylla seeds is more than 99%, and the seedling emergence rate is more than 85%; compared with an existing comprehensive seedling emergence method of the paris polyphylla seeds, the method has the advantage that the seedling emergence rate of the paris polyphylla seeds is increased by 76%.
Description
Technical field
The present invention relates to seed biology techniques field, be specifically related to use different disposal condition to increase Rhizoma Paridis seed sprouting
The method of rate.
Background technology
Rhizoma Paridis (Paris polyphylla var.yunnanensis) is Liliaceae Paris Linnaeus(Paris L.) herbaceos perennial,
It it is the former plant species of medicinal Rhizoma Paridis included of pharmacopeia.Its rhizome has heat-clearing and toxic substances removing, the function of relieving convulsion pain relieving, can be used for laryngopharynx swelling and pain,
Infantile convulsion, venom, furuncle swelling toxin, external treatment furuncle and phyma, parotitis, is the Chinese patent medicines such as YUNNAN BAIYAO, GONGXUENING, sheet son Huang
Primary raw material.For a long time, people rely on wild resource to the utilization of Rhizoma Paridis always, due to the most excessive predation formula
Excavate, wild Rhizoma Paridis has suffered destructive destruction, has been on the verge of exhaustion, artificial growth become solution Rhizoma Paridis resource deficient
Weary inevitable choice.
Rhizoma Paridis seed has hibernation feature, as allowed it naturally sprout, then needs within 15 months, just can emerge, and emergence rate is difficult to
Reach 50%.And using cool stratification, the method for secondary alternating temperature, though can emerge in 1 year, but emergence rate is only 60%.Its reason
It is differentiation not exclusively (form after-ripening) and the plumular axis after-ripening (physiology after-ripening) of Rhizoma Paridis embryo.
Form after-ripening i.e. refers to the stage ripe from seed maturity to embryo, and embryo is slowly growth and sizing in seed.Yunnan weight
The seed gathered after building fruit maturation, is seed maturity.The embryo of mature seed is the most immature, and at this moment the form composition of embryo is the most not
Perfect, it is impossible to germinate.Under suitable epidemic disaster and air conditions, embryo starts differentiation and growth, differentiates cotyledon, plumule, embryo
Axle and radicle, be basically completed the construction of idiosome, and this phase is form after-ripening.After embryo completion morphology after-ripening, give to be suitable for sending out
The environmental condition of bud, seed still can not be unearthed sprouting at once, needs the cryogenic conditions of certain time, and plumular axis is by after its physiology
Ripe, seed could be sprouted.The seed completion morphology after-ripening of Rhizoma Paridis and two stages of physiology after-ripening under field conditions (factors), i.e. from fruit
The real ripe time needing 15 months to seed germination.
Breeding about Rhizoma Paridis, domestic scholars done numerous studies, Li Yunchang (Li Yunchang, Rhizoma Paridis asexual numerous
Growing, Yunnan plant is studied, 1986,8 (4): 429-435) numerous studies have been done in the seminal propagation of Rhizoma Paridis, Rhizoma Paridis seed from
So sprouting, the most treated and be directly sowed at cool canopy seedbed, within 15 months, emergence rate is 46.2%.Li Heng, Yang Xinghua are by Rhizoma Paridis
The Morphological And Anatomical of seed germination is observed: during Rhizoma Paridis seed shedding, embryonic pole aplasia, but one the least, the most completely break up many
Cell ellipsoid, albuminous cell thickness about and keratinization, inner face is filled with starch grain and other nutrient substance, makes endosperm group
Knitting hardening, and be unfavorable for the entrance of air and moisture, the nutrient substance in seed also can not soften the dissolving material for easily absorbing
As nutrition during embryonic development, therefore Rhizoma Paridis seed has been that the differentiation of embryo has latter stage of ripening.Li Yunchang analyzes Rhizoma Paridis
The hibernation feature of seed, after using low temperature, moist preliminary treatment, high-temperature cultivation, although radicle continues to increase, rhizome, plumular axis are also
Develop, but seed still can not break ground and emerge.Being primarily due to the poor growth of plumular axis, there is physiology after ripening in plumular axis, then
Through K cryogenic treatment, then high-temperature cultivation, the growth of plumular axis can be effectively facilitated, seed breaks ground and emerges soon, makes the seed germination time shorten
It is 10～12 months.This research shows, cool stratification can make Rhizoma Paridis embryo preferably grow, and temperature is to affect Rhizoma Paridis Seed Layer
The key factor of long-pending effect.In terms of application exogeneous growth regulator promotes Rhizoma Paridis seed afterripening, most researchs still think suitable
The germination rate processing beneficially Rhizoma Paridis seed when the gibberellins of concentration.
Although many about the research how improving Rhizoma Paridis seed germination rate, but researcher many sight is concentrated on single
Change external condition in the impact of Rhizoma Paridis seed germination rate, and germination efficiency is the most very good, and in terms of emergence rate
Researches.
The present inventor is by the comprehensive mechanism analyzing suppression Rhizoma Paridis seed germination, in conjunction with the feature of seed maturity, invention
A kind of method that can substantially increase Rhizoma Paridis seeding ratio.By twice update search in 2013 to 2015 years, mesh
Before at home and abroad find no the relevant report of this kind of method.
Summary of the invention
For solving the low technical problem of Rhizoma Paridis seeding ratio, what the present invention provided a kind of other method of difference can be significantly
Improve the new method of Rhizoma Paridis seeding ratio (more than 85%).
A kind of method improving Rhizoma Paridis seeding ratio provided by the present invention, its step is as follows:
(1) the ripe Rhizoma Paridis fruit gathered placement is rotted to episperm, then episperm is removed in stranding；
(2) stranding is gone the seed 75%v/v alcohol-pickled 30 that the concentration containing gibberellins is 1000mg/L of episperm
Minute；
(3) the 40～45 DEG C of water soakings 10 hours of the seed after step (2) being processed；
(4) alternating temperature Sand culture, the seed after step (3) being processed, by the ratio that the volume ratio of seed with wet sand is 1:3
Example, mixs homogeneously seed with wet sand, puts into and be placed in growth cabinet in basin, uses 30 DEG C 12h/25 DEG C under dark condition
12h intermittent warming 40～after 50 days, is sown into seedbed by seed, blinding, covers Folium Pini, takes the sunshade net that shading rate is 80%, wet sand
Humid control is 68%～78%.
The present invention is on the basis of the physiological property and summary former achievements of further investigation Rhizoma Paridis seed, by uniqueness
Other people undiscovered method that may advantageously facilitate seed germination and emerge and traditional method promoting Rhizoma Paridis seed germination
Organically integrate, make Rhizoma Paridis seeding ratio reach more than 85%, compared with existing comprehensive seed germination method, seed
Emergence rate improves 76%, creates unforeseeable technique effect.
The innovative point of the inventive method is:
1, placing stranding after episperm rots before episperm is removed in stranding goes episperm, conventional method to be that the ripe Yunnan that will gather is heavy
Building fruit directly rubs episperm with the hands, carries out alternating temperature stratification process after then soaking with gibberellins；The inventive method goes outer kind in stranding
Before skin place episperm rot after rub with the hands remove episperm, it is therefore an objective to allow seed have peel supply nutrient under conditions of, in fruit
Reaching maturity further, this step gathers, with aforementioned routine, the method directly removing episperm after seed, at seed germination rate with go out
Seedling rate raising aspect is much higher, and its seeding ratio improves 31%.
2, use the alcohol-pickled measure containing gibberellins, the seed of episperm will be rubbed with the hands with red mould containing 1000mg/L
75% alcohol-pickled seed half an hour of element, after invade bubble again 10 hours with warm water, add kind of a transdermally, beneficially Exogenous gibberellic acid
Entrance.This measure makes seeding ratio improve 60%.
3, carrying out alternating temperature Sand culture, under dark condition, 30 DEG C 12h/25 DEG C 12h intermittent warming, after 40-50 days, is sown into
Seedbed.This measure makes seed germination rate bring up to 99%, and the emergence rate in later stage reaches more than 85%.
The inventive method instrument is simple, easy to operate, it is easy to carry out batch production Rhizoma Paridis seeling industry.
Detailed description of the invention
Test material used by following example is commercially available.
Embodiment 1 the inventive method
Key instrument: growth cabinet (RGX-250)
Step is as follows:
(1) the ripe Rhizoma Paridis fruit gathered placement is rotted to episperm, then episperm is removed in stranding；
(2) stranding is gone the seed 75%v/v alcohol-pickled 30 that the concentration containing gibberellins is 1000mg/L of episperm
Minute；
(3) by step (2) after the 75%v/v that the concentration containing gibberellins is 1000mg/L processes for alcohol-pickled 30 minutes
Seed 45 DEG C of water soakings 10 hours；
(4) alternating temperature Sand culture, the seed after step (3) being processed for 10 hours with 45 DEG C of water soakings, by seed and wet sand
The ratio that volume ratio is 1:3, seed is mixed homogeneously with wet sand, puts into and basin is placed in growth cabinet, under dark condition
Using the every day of 30 DEG C 12h/25 DEG C 12h intermittent warming after 50 days, seed is sown into seedbed, blinding, cover Folium Pini, taking shading rate is
The sunshade net of 80%, the humid control of described wet sand is 68%.Emergence rate is added up after seed sprouting.Embodiment 1 (side of the present invention
Method) make Rhizoma Paridis seed germination rate reach more than 99%, Rhizoma Paridis seeding ratio reaches more than 85%.
Embodiment 2-embodiment 4 is the comparative experiments by changing monofactor and embodiment 1.
Embodiment 2
Episperm monofactor is directly gone to place to outer kind with ripe Rhizoma Paridis fruit by changing ripe Rhizoma Paridis fruit
Skin rots to rub with the hands to go episperm to compare its impact on Rhizoma Paridis seeding ratio again.
Embodiment 2: in addition to ripe Rhizoma Paridis fruit step (1) gathered directly rubs episperm with the hands, remaining step and reality
Execute example 1 identical.
Test result indicate that (referring to table 1): embodiment 2: ripe Rhizoma Paridis fruit directly rubs episperm with the hands, its Rhizoma Paridis kind
Sub-emergence rate is 67% ± 2%.
The inventive method is than embodiment 2, and its Rhizoma Paridis seeding ratio improves 31.34%, i.e. [(88-67)/76] ×
100%=31.34%.
Embodiment 3
By changing with 1000mg/L gibberellins aqueous solution soaking monofactor and with containing 1000mg/L gibberellins
75% alcohol-pickled compares its impact on Rhizoma Paridis seeding ratio.
Embodiment 3 in addition to the water soaking 30 minutes that step (2) is with 1000mg/L gibberellins, remaining step and embodiment 1
Identical.
Test result indicate that (referring to table 1): the Rhizoma Paridis seeding ratio of embodiment 3 is 58% ± 4%.
The inventive method is than embodiment 3, and its Rhizoma Paridis seeding ratio improves 51%, i.e. [(88-58)/58] ×
100%=51%.
Embodiment 4
It is compared to Rhizoma Paridis seed sprouting with embodiment 1 by Temperature Treatment monofactor during changing seed sand Tibetan
The impact of rate
Embodiment 4 except in step (4) alternating temperature Sand culture temperature be 4 DEG C and process process 60 in 60 days and 20 DEG C beyond the highest heavens, remaining
Step is same as in Example 1.
Test result indicate that (referring to table 1): the Rhizoma Paridis seeding ratio of embodiment 4 is 62% ± 5%.
The inventive method is than embodiment 4, and its Rhizoma Paridis seeding ratio improves 41%, i.e. [(88-62)/62=41%.
Embodiment 5 embodiment 1 the inventive method compares with existing comprehensive Rhizoma Paridis seed germination method
The existing comprehensive Rhizoma Paridis seed sprouting method step of embodiment 5 is as follows:
(1) episperm is removed in stranding, is rubbed with the hands by the ripe Rhizoma Paridis fruit gathered and removes episperm；
(2) by the gibberellins aqueous solution soaking that concentration is 1000mg/L 30 minutes；
(3) alternating temperature Sand culture, presses the body of seed and wet sand by the seed of the gibberellins aqueous solution soaking through 1000mg/L
The long-pending ratio ratio for 1:3, mixs homogeneously seed with wet sand, puts into and be placed in growth cabinet in basin, employing intermittent warming mode:
After 4 DEG C process process in 60 days and 20 DEG C 60 days, seed is sown into seedbed, blinding, covers Folium Pini, take the sunshade that shading rate is 80%
Net, sand humid control 68%.Emergence rate is added up after seed sprouting.
Experiment effect is shown in Table 1: the existing comprehensive Rhizoma Paridis seed sprouting method of embodiment 5, its Rhizoma Paridis seeding ratio is
50% ± 4%.
The inventive method improves 76% than embodiment 5, Rhizoma Paridis seeding ratio, i.e. [(88-50)/50] × 100%
=76%.
Rhizoma Paridis seed germination rate comparable situation under table 1 different disposal method
Claims (1)
1. the method improving Rhizoma Paridis seeding ratio, is characterized in that:
(1) the ripe Rhizoma Paridis fruit gathered placement is rotted to episperm, then episperm is removed in stranding；
(2) stranding is gone the seed of episperm with the 75%v/v that the concentration containing gibberellins is 1000mg/L alcohol-pickled 30 minutes；
(3) the 40～45 DEG C of water soakings 10 hours of the seed after step (2) being processed；
(4) alternating temperature Sand culture, the seed after step (3) is processed, in the ratio that volume ratio is 1:3 of seed Yu wet sand, will
Seed is mixed homogeneously with wet sand, puts into and is placed in growth cabinet in basin, uses 30 DEG C 12h/25 DEG C 12h alternating temperature under dark condition
After processing 40～50 days, seed is sown into seedbed, blinding, covers Folium Pini, take the sunshade net that shading rate is 80%, wet husky humid control
68%～78%.
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| CN201610363884.6A CN105917792B (en) | 2016-05-28 | 2016-05-28 | A method of improving paris polyphylla seeding ratio |
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| CN201610363884.6A CN105917792B (en) | 2016-05-28 | 2016-05-28 | A method of improving paris polyphylla seeding ratio |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107027624A (en) * | 2017-04-28 | 2017-08-11 | 云南农业大学 | A kind of method for obtaining polygerm Yunnan Paris polyphylla seedling |
| CN108040530A (en) * | 2017-12-01 | 2018-05-18 | 亳州学院 | The wild Paris polyphylla seed dormancy in Dabie Mountain breaks method |
| CN111937525A (en) * | 2020-08-11 | 2020-11-17 | 湖南农佳好伙计农业科技发展有限公司 | Paris polyphylla seed sand storage, temperature change and medicament treatment germination accelerating method |
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| CN103222362A (en) * | 2013-04-27 | 2013-07-31 | 云南农业大学 | Method for improving paris polyphylla var. yunnanensis seed germination rate |
| CN104521515A (en) * | 2014-12-29 | 2015-04-22 | 兴安盟京蒙中药材科技开发有限公司 | Method for increasing bud ratio of radix paeoniae rubra seeds |
| CN105210647A (en) * | 2015-10-28 | 2016-01-06 | 云南祥仁生物科技有限公司 | The method for massively culturing of a kind of Yunnan Paris polyphylla seedling |
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| CN104521515A (en) * | 2014-12-29 | 2015-04-22 | 兴安盟京蒙中药材科技开发有限公司 | Method for increasing bud ratio of radix paeoniae rubra seeds |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107027624A (en) * | 2017-04-28 | 2017-08-11 | 云南农业大学 | A kind of method for obtaining polygerm Yunnan Paris polyphylla seedling |
| CN108040530A (en) * | 2017-12-01 | 2018-05-18 | 亳州学院 | The wild Paris polyphylla seed dormancy in Dabie Mountain breaks method |
| CN111937525A (en) * | 2020-08-11 | 2020-11-17 | 湖南农佳好伙计农业科技发展有限公司 | Paris polyphylla seed sand storage, temperature change and medicament treatment germination accelerating method |
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| CN105917792B (en) | 2018-09-21 |
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