CN102972276A - Method for rapidly exercising field melasma herb tissue culture seedlings - Google Patents
Method for rapidly exercising field melasma herb tissue culture seedlings Download PDFInfo
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- 206010008570 Chloasma Diseases 0.000 title claims abstract description 44
- 208000003351 Melanosis Diseases 0.000 title claims abstract description 44
- 230000001954 sterilising effect Effects 0.000 claims abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 12
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 38
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- 230000008569 process Effects 0.000 claims description 6
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- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 4
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Abstract
The invention relates to a method for rapidly exercising field melasma herb tissue culture seedlings and belongs to the technical field of field melasma herb artificial cultivation. The method comprises six technical links of substrate formula selection, substrate sterilization, tissue culture seedling exercising management before seedlings come out of a bottle, bottle opening and seedling cleaning and sterilization, seedling exercising and planting method and management in a seedling exercising stage after planting; the field melasma herb tissue culture seedlings can be exercised and domesticated for 15-20 d; after the exercised and domesticated tissue culture seedlings are transplanted to big fields, the survival rate of the tissue culture seedlings is more than 95 %; the method provided by the invention has the advantages of high seedling exercising and domesticating speed, high seedling percentage and so on; and problems of lower field melasma herb tissue culture seedling transplanting survival rate, slow growth and the like are solved. The method provided by the invention has the advantages of simplicity, lower cost and convenience in operation and is suitable for being applied to actual production.
Description
Technical field:
The present invention relates to a kind of method of method, particularly a kind of Wild Medicinal field melasma herb ［ Melasma arvense (Benth.) Hand.-Mazz. ］ tissue culture sprout quick speed hardening of field melasma herb tissue culture sprout quick speed hardening.Belong to Wild Medicinal artificial planting technique field.
Background technology:
Field melasma herb is the root of the black capsule [Melasma arvense (Benth.) Hand.-Mazz.] of goatweed, it is herbaceos perennial, mainly be distributed in the southern areas, Yunnan Province, be the rare medicinal materials of national folk such as Yunnan Province Miao ethnic group, the Yi nationality, distributed over Yunnan, Sichuan and Guizhou, the Lahu, Duo Jia hospital in Yunnan is mainly used in treating leukemia, tumour, cardiovascular and cerebrovascular diseases, icteric hepatitis, hepatomegaly, falls the diseases such as the stasis of blood is swollen, dysmenorrhoea of injuring.Field melasma herb has can be developed as the early stage leukemia for the treatment of, antitumor and change the potentiality of blood, haemolysis special effect medicine.For a long time, the field melasma herb crude drug source all depends on wild plant resource, and along with the demand in natural medicine market grows with each passing day, the field melasma herb wild resource is subject to heavy damage, and wild resource has been difficult to find at present.
The field melasma herb Regional Distribution is narrow, seed nature germination rate extremely low (less than 0.3%), group training and group training transplantation of seedlings domestication difficulty, it is large that difficulty is planted by wild change man, the formula of in addition robbing is in recent years excessively excavated, the herb fresh goods surpasses 4000 yuan/kilogram at present, is in out of stock in spite of rising price, extremely rare, endangered.It is carried out protection of resources to urgent need and research is planted by wild change man.
By literature search, yet there are no any relevant field melasma herb seedling Fast-propagation, wild resource investigation, seed germination, wild change man and the content of publishing such as plant.
Summary of the invention:
The method that the object of the present invention is to provide a kind of field melasma herb tissue culture sprout quick speed hardening to survive.This method is washed the method that environment conditioning combines after seedling sterilization, field planting method and the field planting by management, the uncork before the training seedling bottle outlet disinfected, organize of matrix rational formula, matrix, can realize effectively that field melasma herb tissue culture sprout quick speed hardening survives.
The method of a kind of field melasma herb tissue culture sprout quick speed of the present invention hardening domestication is achieved through the following technical solutions, and comprises the following step:
A. matrix is selected: after mixing according to peat, ash, fine sand 3:1:1, excessively behind 120 mesh sieves, be prepared into field planting matrix;
B. matrix is disinfected: ready matrix is spread out, to 2000 times of carbendazim solutions of surface sprinkling, amount by matrix and liquid 50-80:1 is sprayed, mix while spraying, after having sprayed matrix piled heap, cover the vexed heap of sealing with Polypropylence Sheet, for subsequent use in after 10-15 days the matrix after sterilization packed into nutritious bag or the seedling culture hole plate;
C. the hardening management before the group training seedling bottle outlet: when the height of seedling degree reaches more than the 5cm, 4-5 bar root is arranged, root reaches 0.5cm when above, reaches the seedling standard, can carry out hardening; Be transferred to room temperature with the bottle seedling this moment, and intensity of illumination 2000～3000lx cultivates under the condition of light application time 10h/d about 20d and can uncork draw seedling;
D. seedling sterilization is washed in uncork: 2d before the seedling is drawn in uncork, opens the cultivation bottle cap, can draw seedling after allowing seedling fully adapt to Indoor Temperature, humidity; Carefully seedling in the tissue culture bottle is drawn out, cleaned medium with 15-18 ℃ of clear water, then with behind the 0.001% liquor potassic permanganate bubble root 15min, again with the clean liquid of 15-18 ℃ of clear water, wait for field planting after drying steam;
E, hardening field planting method: will install the nutritious bag of matrix or cave dish and move into the greenhouse fixedly in the seedbed, water permeablely, with field planting after the tweezers punching, use the clear water repeated spray after the field planting, spray saturating till; After field planting is complete, take bow member at nutritious bag or cave dish, covered with sunshade net shelters from heat or light;
Hardening period management after F, the field planting: the peripheral environment of hardening is greenhouse or booth, shelter from heat or light about 85% during the hardening, keep illuminance between 3000-4000Lx, temperature 24-26 ℃, between the air humidity 75-85%, 1-3 the moisturizing of spraying water every day can be transplanted to large Tanaka after finishing hardening domestication process behind the 10-20d.
Described matrix is selected, matrix is disinfected, seedling is washed in uncork, hardening period management after hardening field planting method and the field planting.
The invention has the advantages that: wash six sport technique segments operations of management after seedling sterilization, field planting method and the field planting by management, uncork before the disinfecting of matrix rational formula, matrix, the bottle outlet, can realize field melasma herb group training seedling after through 10-20d left and right sides hardening domestication, be transplanted to the land for growing field crops and can be held in motility rate and reach more than 90%.The invention belongs to field melasma herb artificial cultivation technique field, the technology of the present invention method is simple, and cost is lower, and convenient operation is suitable for production application.
Embodiment
In order to make purpose of the present invention, technical scheme clearer, the present invention tames management method, uncork before Screening matrix, the uncork of group training seedling with instantiation to hardening and washes the research screening process of the technology such as light warm water regulation and control after seedling sterilization, the field planting and carried out sufficient explanation, proving the correctness of this achievement in research, but be not that explanation the present invention is only for being used for these examples.
The method of a kind of field melasma herb ［ Melasma arvense (Benth.) Hand.-Mazz. ］ tissue culture sprout quick speed hardening comprises the following step:
A. matrix is selected: after mixing according to peat, ash, fine sand 3:1:1, excessively behind 120 mesh sieves, be prepared into field planting matrix.
B. matrix is disinfected: ready matrix is spread out, to 2000 times of carbendazim solutions of surface sprinkling, amount by matrix and liquid 50-80:1 is sprayed, mix while spraying, after having sprayed matrix piled heap, cover the vexed heap of sealing with Polypropylence Sheet, for subsequent use in after 10-15 days the matrix after sterilization packed into nutritious bag or the seedling culture hole plate.
C. the hardening management before the group training seedling bottle outlet: when the height of seedling degree reaches more than the 5cm, 4-5 bar root is arranged, root reaches 0.5cm when above, reaches the seedling standard, can carry out hardening.Be transferred to room temperature with the bottle seedling this moment, and intensity of illumination 2000～3000lx cultivates under the condition of light application time 10h/d about 20d and can uncork draw seedling.
D. seedling sterilization is washed in uncork: 2d before the seedling is drawn in uncork, opens the cultivation bottle cap, can draw seedling after allowing seedling fully adapt to Indoor Temperature, humidity; Carefully seedling in the tissue culture bottle is drawn out, cleaned medium with 15-18 ℃ of clear water, then with behind the 0.001% liquor potassic permanganate bubble root 15min, again with the clean liquid of 15-18 ℃ of clear water, wait for field planting after drying steam.
E, hardening field planting method: will install the nutritious bag of matrix or cave dish and move into the greenhouse fixedly in the seedbed, water permeablely, with field planting after the tweezers punching, use the clear water repeated spray after the field planting, spray saturating till; After field planting is complete, take bow member at nutritious bag or cave dish, covered with sunshade net shelters from heat or light.
Hardening period management after F, the field planting: the peripheral environment of hardening is greenhouse or booth.Shelter from heat or light about 85% during the hardening, keep illuminance between 3000-4000Lx, temperature 24-26 ℃, between the air humidity 75-85%, 1-3 the moisturizing of spraying water every day can be transplanted to large Tanaka after finishing hardening domestication process behind the 10-20d.
The experimental study of field melasma herb training tissue culture seedling domestication preference temperature
In order to explore the suitable temperature condition of field melasma herb training tissue culture seedling domestication.This research is carried out hardening domestication comparative test research by 4 kinds of different rooting culture temperature (20 ± 2,24 ± 2,28 ± 2,30 ± 2) are set under the identical condition of other method of operating.Study all processing and choose the similar seedling of growing way (height 6-7cm, 4-5 bar root, the long 0.4-0.5cm of root, 4-6 leaf) and divide into groups, every group of 10 bottles of 100 seedlings are established 5 repetitions, and every repetition 20 seedlings are numbered respectively.20d before preparing hardening is transferred to room temperature with the bottle seedling after the numbering, at intensity of illumination 2000～3000lx, cultivates 20d under the condition of illumination 12h/d and can uncork transplant; 2d before the seedling is drawn in uncork, opens the cultivation bottle cap, can draw seedling after allowing seedling fully adapt to Indoor Temperature, humidity, the seedling of drawing out is cleaned medium with 15-18 ℃ of clear water, then with behind the 0.001% liquor potassic permanganate bubble root 15min, clean liquid with 15-18 ℃ of clear water again, wait for field planting after drying steam; Little molehill stone and humus soil 1:1 mixing is rear as domestication matrix, water permeable rear field planting in the hardening of packing into the dish.All placing the artificial intelligence illumination cultivation of different temperatures to carry out hardening the seedling after the field planting cultivates, hardening phase intensity of illumination is 2000～3000Lx, illumination 12h/d, air humidity 75-85%, 1-3 the moisturizing of spraying water every day after 10 days, observed statistics domestication survival rate and growth situation in field planting, get after 20 days and be transplanted to the land for growing field crops after seedling is measured root length, plant height and blade, add up transplant survival behind the 45d.
Result of study shows that growth and the transplanting situation of processing 2 seedlings are best, is embodied in domestication survival rate, growing state, average root length, blade increase number and field-transplanting and all significantly is better than other processing; It is the poorest to process 1 effect, and its domestication survival rate, growing state, average root length, plant height value added, blade increase number and field-transplanting all significantly are lower than other processing.By above presentation of results, temperature is very large to the hardening domestication influential effect of field melasma herb test-tube plantlet, and the temperature that its hardening domestication suits is at 22-26 ℃.
The growth of seedling and transplanting situation under table 1 different temperatures
Annotate: samely list different letters, be illustrated respectively in the significance of difference on P＜0.05 level; Data are the mean of 5 repetitions in the table.
The suitable intensity of illumination experimental study of field melasma herb training tissue culture seedling domestication
In order to explore the suitable intensity of illumination of field melasma herb training tissue culture seedling domestication.This research is carried out hardening domestication comparative test research by 4 kinds of different intensities of illumination (1000-2000lx, 3000-4000lx, 5000-6000lx, 8000-10000lx) are set under the identical condition of other method of operating.Study all processing and choose the basically identical seedling of growing way (height 6-7cm, 4-5 bar root, the long 0.4-0.5cm of root, the 4-6 leaf) field planting of dividing into groups, every group of 10 bottles of 100 seedlings, numbering.20d before the preparation hardening is transferred to room temperature with the bottle seedling after the selection numbering, at intensity of illumination 2000～3000lx, cultivates 20d under the condition of illumination 12h/d and gets final product the uncork transplanting; The front 2d of seedling is drawn in uncork, open the cultivation bottle cap, can draw seedling after allowing seedling fully adapt to Indoor Temperature, humidity, 2d can draw out seedling after the uncork, the seedling of drawing out is cleaned medium with 15-18 ℃ of clear water, then with behind the 0.001% liquor potassic permanganate bubble root 15min, clean liquid with 15-18 ℃ of clear water again, wait for field planting after drying steam; Little molehill stone and humus soil 1:1 mixing is rear as domestication matrix, water permeable rear field planting in the hardening of packing into the dish.
All placing the artificial intelligence illumination box of different illumination intensity to carry out hardening the seedling after the field planting cultivates, illumination 12h/d, air humidity 75-85%, temperature 22-26 ℃, 1-3 the moisturizing of spraying water every day after 10 days, observed statistics domestication survival rate and growth situation in field planting, get after 20 days and be transplanted to the land for growing field crops after seedling is measured root length, plant height and blade, add up transplant survival behind the 45d.
Result of study shows that growth and the transplanting situation of processing 2 seedlings are best, is embodied in domestication survival rate, growing state, average root length, blade increase number and field-transplanting and all significantly is better than other processing; It is the poorest to process 4 effects, and its domestication survival rate, growing state, average root length, plant height value added, blade increase number and field-transplanting all significantly are lower than other processing; Intensity of illumination is very large to the hardening of field melasma herb test-tube plantlet domestication influential effect, although that the strong low hardening domestication of illumination survives efficient is higher, excessive growth, seedlings root undergrowth, blade can occur increases slow, the false problem such as live; Intensity of illumination is excessive, survival rate reduction, seedling, seedlings root undergrowth, blade increase can occur and wait slowly problem; Be 3000-4000lx by optimum intensity of illumination between above presentation of results field melasma herb hardening domestication.
The growth of seedling and transplanting situation under table 2 different illumination intensity
Annotate: samely list different letters, be illustrated respectively in the significance of difference on P＜0.05 level; Data are the mean of 5 repetitions in the table.
The experimental study of field melasma herb training tissue culture seedling domestication Screening matrix
In order to explore the suitable matrix of field melasma herb training tissue culture seedling domestication.This research is under the identical condition of other method of operating, by 5 kinds of different matrix (earth: fertilizer 4:1, humus soil, humus soil: little molehill stone 4:1, ash: earth 5:1, peat: ash: fine sand 3:1:1) carry out hardening domestication comparative test research is set.Study all processing and choose the basically identical seedling of growing way (height 6-7cm, 4-5 bar root, the long 0.5-0.7cm of root, the 4-6 leaf) field planting of dividing into groups, every group of 10 bottles of 100 seedlings, numbering.20d before the preparation hardening is transferred to room temperature with the bottle seedling after the selection numbering, at intensity of illumination 2000～3000lx, cultivates 20d under the condition of illumination 12h/d and gets final product the uncork transplanting; The front 2d of seedling is drawn in uncork, open the cultivation bottle cap, can draw seedling after allowing seedling fully adapt to Indoor Temperature, humidity, 2d can draw out seedling after the uncork, the seedling of drawing out is cleaned medium with 15-18 ℃ of clear water, then with behind the 0.001% liquor potassic permanganate bubble root 15min, clean liquid with 15-18 ℃ of clear water again, wait for field planting after drying steam; Different matrix packed into water permeable rear field planting in the hardening dish.
All placing the artificial intelligence illumination box of different illumination intensity to carry out hardening the seedling after the field planting cultivates, intensity of illumination 3000-4000lx, illumination 12h/d, temperature 22-26 ℃, air humidity 75-85%, 1-3 the moisturizing of spraying water every day, in field planting after 10 days, observe statistics domestication survival rate and growth situation, get after 20 days and be transplanted to the land for growing field crops after seedling is measured root length, plant height and blade, add up transplant survival behind the 45d.
Result of study shows that growth and the transplanting situation of processing 4 and processing 5 seedlings are best, is embodied in domestication survival rate, growing state, average root length, blade increase number and field-transplanting and all significantly is better than other processing, and the two is without significant difference; It is the poorest to process 1 effect, and its domestication survival rate, growing state, average root length, plant height value added, blade increase number and field-transplanting survival rate all significantly are lower than other processing; Matrix formulations is very large to the hardening domestication influential effect of field melasma herb test-tube plantlet, domestication survival rate, growth of seedling situation, average root length, plant height value added, blade are increased number to different matrix and the impact of field-transplanting survival rate is remarkable, should add the ash of some by matrix between above presentation of results field melasma herb hardening domestication, its suitable matrix formulations is peat: ash: fine sand 3:1:1.
Growth and the transplanting situation of the lower seedling of table 3 different substrates hardening domestication
Annotate: samely list different letters, be illustrated respectively in the significance of difference on P＜0.05 level; Data are the mean of 5 repetitions in the table.
The preprocess method research of field melasma herb training tissue culture seedling domestication seedling
In order to explore the preprocess method of seedling in the domestication of field melasma herb training tissue culture seedling, successfully set up the preprocess method of field melasma herb training tissue culture seedling domestication kind of the seedling of standard.This research by the pretreatment mode of 4 kinds of different seedlings is set, is carried out hardening domestication comparative test research in matrix formulations, the identical situation of hardening domestication environment conditioning (temperature, intensity of illumination, light application time, water management).Shown in 4 kinds of different seedling preprocess methods are specific as follows:
Method 1: directly from culturing room seedling is taken out, draw immediately seedling, behind the clean medium, directly field planting;
Method 2: before drawing seedling the bottle seedling is transferred under the room temperature from culturing room, at intensity of illumination 1200～2000lx, illumination 10h/d cultivates 20d; Then draw seedling, field planting behind the clean medium;
Method 3: before drawing seedling the bottle seedling is transferred under the room temperature from culturing room, at intensity of illumination 2000～3000lx, illumination 12h/d cultivates 18d; Then draw seedling after opening bottle cap 2d, will clean field planting behind the medium;
Method 4: before drawing seedling the bottle seedling is transferred under the room temperature from culturing room, at intensity of illumination 2000～3000lx, illumination 12h/d cultivates 18d; Then draw seedling after opening bottle cap 2d, clean medium with 15-18 ℃ of clear water after, again with 0.001% liquor potassic permanganate bubble root 15min, at last again with the clean liquid of 15-18 ℃ of clear water, dry field planting behind the steam.
Study all processing and all choose the basically identical seedling of growing way (height 6-7cm, 4-5 bar root, the long 0.5-0.7cm of root, the 4-6 leaf) field planting of dividing into groups, every group of 10 bottles of 100 seedlings, numbering.With peat: ash: fine sand (3:1:1) mixes as domestication matrix, waters permeable rear field planting in the hardening of packing into the dish.
All placing the artificial intelligence illumination box of different illumination intensity to carry out hardening the seedling after the field planting cultivates, during the hardening temperature 22-26 ℃, intensity of illumination 3000-4000lx, illumination 12h/d, air humidity 75-85%, 1-3 the moisturizing of spraying water every day, in field planting after 10 days, observe statistics domestication survival rate and growth situation, get after 20 days and be transplanted to the land for growing field crops after seedling is measured root length, plant height and blade, add up transplant survival behind the 45d.
Result of study shows that growth and the transplanting situation of processing 4 seedlings are best, and it is high to be embodied in the domestication survival rate, growth of seedling is normal, without going rotten, occuring without the phenomenon such as wilting, the domestication seedling to be analyzed, its average root is long, blade increase number and field-transplanting all significantly are better than other processing; Other the three kinds of methods all phenomenon such as mildew and rot, the wilting and introduced disease of in various degree appearance occur.Pretreated program by seedling between above presentation of results field melasma herb hardening domestication is: draw Miao Qianxian the bottle seedling is transferred under the room temperature from culturing room, at intensity of illumination 2000～3000lx, illumination 10-12h/d cultivates 18d; Then draw seedling after opening bottle cap 2d, clean medium with 15-18 ℃ of clear water after, with 0.001% liquor potassic permanganate bubble root 15min, clean liquid with 15-18 ℃ of clear water more at last again, dry ability field planting behind the steam.
Growth and the transplanting situation of different seedling preprocess methods before the domestication of table 4 hardening
Annotate: samely list different letters, be illustrated respectively in the significance of difference on P＜0.05 level; Data are the mean of 5 repetitions in the table.
The fast hardening Optimum Experiment research in booth of field melasma herb group training seedling
In order further to optimize the method for field melasma herb training tissue culture seedling domestication, outstanding land for growing field crops operability.This research is regulated and not to be coexisted seedling pre-treatment, matrix formulations, temperature adjusting all in the constant situation, in the booth of temperature controllable, humidity, utilize natural daylight, change shading rate (85%, 75%, 60%, 45%) by sunshade net, under the identical condition of other method of operating, carry out hardening domestication comparative test research.
This is studied all processing and chooses the basically identical seedling of growing way (height 6-7cm, 4-5 bar root, the long 0.5-0.7cm of root, the 4-6 leaf) field planting of dividing into groups, every group of 10 bottles of 100 seedlings, numbering.20d before the preparation hardening is transferred to room temperature with the bottle seedling after the selection numbering, at intensity of illumination 2000～3000lx, cultivates 20d under the condition of illumination 12h/d and gets final product the uncork transplanting; The front 2d of seedling is drawn in uncork, open the cultivation bottle cap, can draw seedling after allowing seedling fully adapt to Indoor Temperature, humidity, 2d can draw out seedling after the uncork, the seedling of drawing out is cleaned medium with 15-18 ℃ of clear water, then with behind the 0.001% liquor potassic permanganate bubble root 15min, clean liquid with 15-18 ℃ of clear water again, wait for field planting after drying steam; Before hardening operation 10-15 days, with peat: ash: fine sand (3:1:1) mixes as matrix, ready matrix is spread out, to 2000 times of carbendazim solutions of surface sprinkling, spray by the amount of matrix and liquid 50-80:1, mix while spraying, after having sprayed matrix piled heap, cover the vexed heap of sealing with Polypropylence Sheet, in the seedling culture hole plate of after 10-15 days the matrix after sterilization being packed into, water permeable rear wait field planting.
Carrying out hardening when the seedling after the field planting is transferred in the booth cultivates, take different shading rates, greenhouse temperature control is 22-26 ℃ during the hardening, 1-3 the moisturizing of spraying water every day, keep air humidity 75-85%, after 10 days, observe statistics domestication survival rate and growth situation in field planting, get after 20 days and be transplanted to the land for growing field crops after seedling is measured root length, plant height and blade, add up transplant survival behind the 45d.
Result of study shows, growth and the transplanting situation of processing 1 seedling are best, it is high to be embodied in the domestication survival rate, the leaf look dark green, stem more slightly is aubergine, leaf is open and flat, and a small amount of branch occurs, and growth of seedling is healthy and strong, the domestication seedling is analyzed, and its average root is long, blade increases number and field-transplanting all significantly is better than other processing; It is the poorest to process 4 effects, and its domestication survival rate, growing state, average root length, plant height value added, blade increase number and all significantly be lower than other processing; By several processing are compared analysis, the difference of the growth of seedling between each is processed be mainly manifested in hardening domestication survival rate and strong sprout the rate variance heteropole large, significant variation has also appearred in the every growth indexes of seedling.In the booth of controllable temperature, carry out hardening domestication operation by above presentation of results field melasma herb, require suitable shading rate about 85%.
Growth and the transplanting situation of the different shading rate condition of table 5 lower refining seedling domestication seedling
Annotate: samely list different letters, be illustrated respectively in the significance of difference on P＜0.05 level; Data are the mean of 5 repetitions in the table.
The method that adopts field melasma herb tissue culture sprout quick speed hardening of the present invention to survive, through hardening domestication in 15-20 days, the survival rate after the domestication after the group training transplantation of seedlings reached more than 95%, has produced great economic benefit and social benefit.
Claims (2)
1. the method for field melasma herb tissue culture sprout quick speed hardening is characterized in that comprising the following step:
A. matrix is selected: after mixing according to peat, ash, fine sand 3:1:1, excessively behind 120 mesh sieves, be prepared into field planting matrix;
B. matrix is disinfected: ready matrix is spread out, to 2000 times of carbendazim solutions of surface sprinkling, amount by matrix and liquid 50-80:1 is sprayed, mix while spraying, after having sprayed matrix piled heap, cover the vexed heap of sealing with Polypropylence Sheet, for subsequent use in after 10-15 days the matrix after sterilization packed into nutritious bag or the seedling culture hole plate;
C. the hardening management before the group training seedling bottle outlet: when the height of seedling degree reaches more than the 5cm, 4-5 bar root is arranged, root reaches 0.5cm when above, reaches the seedling standard, can carry out hardening; Be transferred to room temperature with the bottle seedling this moment, and intensity of illumination 2000～3000lx cultivates under the condition of light application time 10h/d about 20d and can uncork draw seedling;
D. seedling sterilization is washed in uncork: 2d before the seedling is drawn in uncork, opens the cultivation bottle cap, can draw seedling after allowing seedling fully adapt to Indoor Temperature, humidity; Carefully seedling in the tissue culture bottle is drawn out, cleaned medium with 15-18 ℃ of clear water, then with behind the 0.001% liquor potassic permanganate bubble root 15min, again with the clean liquid of 15-18 ℃ of clear water, wait for field planting after drying steam;
E, hardening field planting method: will install the nutritious bag of matrix or cave dish and move into the greenhouse fixedly in the seedbed, water permeablely, with field planting after the tweezers punching, use the clear water repeated spray after the field planting, spray saturating till; After field planting is complete, take bow member at nutritious bag or cave dish, covered with sunshade net shelters from heat or light;
Hardening period management after F, the field planting: the peripheral environment of hardening is greenhouse or booth, shelter from heat or light about 85% during the hardening, keep illuminance between 3000-4000Lx, temperature 24-26 ℃, between the air humidity 75-85%, 1-3 the moisturizing of spraying water every day can be transplanted to large Tanaka after finishing hardening domestication process behind the 10-20d.
2. according to the method for field melasma herb tissue culture sprout quick claimed in claim 1 speed hardening, it is characterized in that described matrix is selected, matrix is disinfected, seedling is washed in uncork, hardening period management after hardening field planting method and the field planting.
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| CN107155844A (en) * | 2017-05-16 | 2017-09-15 | 山东博华高效生态农业科技有限公司 | A kind of method of Tissue Culture of Trees seedling greenhouse hardening domestication |
| CN107864865A (en) * | 2017-12-26 | 2018-04-03 | 丽江市古城区秋成种养殖有限公司 | A kind of efficient hardening technology and domesticating cultivation method of haw ginseng tissue-cultured seedling |
| CN108207553A (en) * | 2017-12-29 | 2018-06-29 | 西安文理学院 | A kind of dedicated soilless culture substrate of ornamental sunflower nursery and preparation method thereof |
| CN108371030A (en) * | 2017-12-27 | 2018-08-07 | 康美药业（文山）药材种植管理有限公司 | A kind of method for transplanting of red root wild silkworm beans tissue-cultured seedling |
| CN108575670A (en) * | 2018-02-02 | 2018-09-28 | 青岛市农业科学研究院 | A kind of apple rootstock tissue culture method for transplanting |
| CN110352804A (en) * | 2019-08-15 | 2019-10-22 | 江西环境工程职业学院 | A kind of acclimatization and transplants method of savatier monochasma herb |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107155844A (en) * | 2017-05-16 | 2017-09-15 | 山东博华高效生态农业科技有限公司 | A kind of method of Tissue Culture of Trees seedling greenhouse hardening domestication |
| CN107864865A (en) * | 2017-12-26 | 2018-04-03 | 丽江市古城区秋成种养殖有限公司 | A kind of efficient hardening technology and domesticating cultivation method of haw ginseng tissue-cultured seedling |
| CN108371030A (en) * | 2017-12-27 | 2018-08-07 | 康美药业（文山）药材种植管理有限公司 | A kind of method for transplanting of red root wild silkworm beans tissue-cultured seedling |
| CN108207553A (en) * | 2017-12-29 | 2018-06-29 | 西安文理学院 | A kind of dedicated soilless culture substrate of ornamental sunflower nursery and preparation method thereof |
| CN108575670A (en) * | 2018-02-02 | 2018-09-28 | 青岛市农业科学研究院 | A kind of apple rootstock tissue culture method for transplanting |
| CN110352804A (en) * | 2019-08-15 | 2019-10-22 | 江西环境工程职业学院 | A kind of acclimatization and transplants method of savatier monochasma herb |
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