CN107155844A - A kind of method of Tissue Culture of Trees seedling greenhouse hardening domestication - Google Patents
A kind of method of Tissue Culture of Trees seedling greenhouse hardening domestication Download PDFInfo
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- CN107155844A CN107155844A CN201710345382.5A CN201710345382A CN107155844A CN 107155844 A CN107155844 A CN 107155844A CN 201710345382 A CN201710345382 A CN 201710345382A CN 107155844 A CN107155844 A CN 107155844A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The present invention relates to a kind of method of Tissue Culture of Trees seedling greenhouse hardening domestication, it is characterised in that comprises the steps of：(1) hardening greenhouse prepares；(2) hardening is managed before tissue-cultured seedling bottle outlet；(3) cultivation matrix prepares with container；(4) tissue culture transplantation of seedlings；(5) management is tamed after transplanting；(6) time control is tamed after transplanting.Tissue Culture of Trees seedling greenhouse hardening acclimation method of the present invention; iris production used forms the domestication environment of more uniform stabilization with white clear nutritive cube and correspondingly sized hole tray; compared with prior art; simple and efficient; and hole tray and nutritive cube recoverable; production cost is greatly reduced, can large-scale promotion；Measure resultant effect before hardening of the present invention, in hardening and after hardening, hardening domestication time at least one month after being transplanted in Tissue Culture of Trees seedling greenhouse can be reduced, hardening survival rate improves 5 15 percentage points, artificial and Material Cost averagely reduces by 30% or so, the more conventional hardening off method of tissue-cultured seedling aerial growth amount improves 30 50%, and plant strain growth is healthy and strong.
Description
Technical field
The invention belongs to forest quick breeding technology field, it is related to a kind of simple height of Tissue Culture of Trees seedling greenhouse hardening domestication
Efficacious prescriptions method.
Background technology
Tissue Culture of Trees nursery is that aseptically, artificial create is adapted to nutrition and environment bar needed for tree growth development
Part, cultivates the tissue or organ of xylophyta, makes an independent plants.The mode of Tissue Culture of Trees nursery is particularly suitable for use in numerous
Rare tree species or the excellent garden-variety of maternal character are grown, and because it can obtain virus-elimination seedlingses, the preservation available for germ plasm resource
With the production of high-quality virus-elimination seedlingses, there is important meaning to the fine-variety breeding for promoting forest tree.Tree Organization culture is de-
Malicious seedling is cultivated and rapid propagation in factory aspect will be widely used.
Many forest trees have been successfully established tissue culture rapid propagation system, but forest tree has the characteristics of growth cycle is long,
Browning, vitrifying in some problems, such as incubation are still suffered from tissue culture, pollute and take root difficulty the problem of, hardening
Domestication and transplanting survival rate are low, and tissue culture cost is excessively high.These factors constrain Tissue Culture of Trees nursery production in realize scale,
Industrialization.
Research for Tissue Culture of Trees seedling hardening and transplanting is deepening continuously, in terms of hardening domestication, first by group of having taken root
Training seedling is placed on culturing room or greenhouse is uncapped 1~7d of hardening, tissue-cultured seedling is gradually adapted to the external world collarium border；Then root is not being hindered
On the premise of clean root with clear water；By tissue-cultured seedling plant into the container equipped with matrix, matrix often for turf, perlite, vermiculite,
Several in garden mould, river sand etc. are well mixed by a certain percentage, and with carbendazim, Mancozeb, Bravo or potassium permanganate etc.
Sterilizing in advance, according to tissue-cultured seedling size is transplanted, container commonly uses different size of hole tray, nutritive cube and double-colored basin etc.；It will move
The tissue-cultured seedling planted into container is placed on hardening in intelligent greenhouse or plastic canopy and tamed, according to different forest species, control
Suitable humiture and illumination condition, are controlled between 20-30 DEG C temperature, humidity range is generally 50-80%, natural striation more
Shading treatment under part；Transplant after domestication tens days to some months, you can reach that field planting or sale are required.Deposited in current hardening domestication
The problem of have:(1) to ensure survival rate, in-bottle seeding overlong time influences seedling efficiency；(2) environmental condition control is cumbersome,
Cost is too high.Transplanting the tissue-cultured seedling of hardening needs to carry out in the facility such as intelligent greenhouse or shed, temperature, moisture, illumination etc.
Control must be fine, otherwise easy rotten, and survival rate declines.
The content of the invention
Not enough in order to solve prior art, the invention provides a kind of refining for realizing simple and efficient in Tissue Culture of Trees seedling greenhouse
Seedling acclimation method.Comprise the steps of：
(1) hardening greenhouse prepares：The conventional manageable glasshouse of epidemic disaster is selected, there is inside and outside sunshade net, seedbed
Arrange, seedbed and surrounding environment spray 300-500 times of carbendazim solution sterilization；
(2) hardening is managed before tissue-cultured seedling bottle outlet：Robust plant, and neat and consistent of taking root are selected, the group for the standard of emerging is reached
Seedling is trained, tissue culture bottle where the tissue-cultured seedling is moved on hardening greenhouse seedbed, intensity of illumination is controlled in the range of 3000-5000lx,
Temperature is generally 18-30 DEG C, humidity 60-80%, after hardening 1-3d, tissue culture bottle cap is turned on but not taken down, and continues hardening 4-
6d；
Tissue culture bottle cap is turned on but not taken down, can so stablize the humiture of training tissue culture seedling environment, preceding place is allowed to
Tissue-cultured seedling under constant temperature and humidity sterile culture environment progressively adapts to external environment, improves hardening success rate；
(3) cultivation matrix prepares with container：The turfy soil and perlite of routine is selected to be used as cultivation matrix, turf and pearl
Rock volume ratio is 1:1~3:2, suitable hole tray, generally 15 holes, 21 holes, 32 holes, 50 holes are selected according to tissue-cultured seedling size
Disk, loads matrix, floating, with 800-1000 times of carbendazim solution spray disinfectant, selects iris special white according to hole tray size
The transparent nutritive cube of color, with 1000 times of carbendazim solution soaking disinfections, specification is generally 1.5 cun~2.5 cun；
Scale application in the production of iris special white clear nutritive cube, it is complete in specifications, it is readily available, and can be with
Recycle；
(4) tissue culture transplantation of seedlings：The tissue-cultured seedling that step (2) is obtained is taken out with tweezers, and clear water is used on the premise of root is not hindered
Culture medium is rinsed well, tissue-cultured seedling root is put into 1000 times of carbendazim solutions and soaks 2min, takes out fixed after somewhat airing
Plant in the hole tray after step (3) processing, root system is unfolded during field planting, and matrix is compacted, matrix is away from edge about 1- in hole tray after compacting
2cm, plant root does not expose, and base portion exposes 0.5-1cm, pours permeable with spray mode, by correspondingly sized white clear nutritive cube
Tip upside down in the hole tray after field planting；
(5) management is tamed after transplanting：
Water management：Warm indoor environment moisture management refers to prior art, typically in the range of 60-80%, and hole tray is with falling
Metastable subenvironment is formed between the nutrition cup of button, without water spray during domestication, permeable aperture, back-off are arranged at nutritive cube bottom
While maintaining matrix with ambient humidity in hole tray, the humidity in subenvironment is unlikely to excessive, cause rotten, and it is unlikely
Illumination is influenceed in formation water smoke；
Illumination management：7-15d before after transplanting, light intensity is controlled in 5000-8000lx by the use of sunshade net inside and outside greenhouse
Between, the domestication of external sunshade net is opened afterwards；
Temperature treatment：Different according to the forest species transplanted, warm indoor temperature general control is between 18-30 DEG C；
(6) the domestication time is generally 25-45d after transplanting, and afterwards removes the transparent nutritive cube of back-off, water management is changed to
Periodically spray water, you can reach the requirement for changing basin production nutrition pot seedling or direct field-transplanting.
Carbendazim solution use herein is formulated by active ingredient for 50% carbendazol wettable powder,
Such as 1000 times of carbendazim solutions are the 1000g carbendazim being formulated by the carbendazol wettable powder that 1g active ingredients are 50%
Solution；
The invention provides a kind of Tissue Culture of Trees seedling greenhouse hardening acclimation method of simple and efficient, with advantages below：
(1) seedbed and surrounding environment are sterilized with high concentration carbendazim before hardening of the present invention, compared with prior art, reduction
The probability of tissue-cultured seedling sense bacterium, improves the survival rate and seedling quality of nursery stock；
(2) present invention forms taming and dociling for more uniform stabilization with white clear nutritive cube and correspondingly sized hole tray with iris production
Change environment, compared with prior art, the present invention is simpler easy, and hole tray and nutritive cube recoverable, greatly reduces
Production cost, can large-scale promotion；
(3) the measure resultant effect before hardening of the present invention, in hardening and after hardening, can reduce Tissue Culture of Trees seedling greenhouse
Hardening tames time at least one month after interior transplanting, and hardening survival rate improves 5-15 percentage points, and artificial and Material Cost is flat
30% or so is reduced, the more conventional hardening off method of tissue-cultured seedling increment improves 30-50%, plant strain growth is healthy and strong.
Brief description of the drawings
Fig. 1 is that the special white clear nutritive cube of iris of the present invention uses schematic diagram.
Reference
1 is nutritive cube in figure, and 2 be hole tray.
Embodiment
Following examples further illustrate the specific implementation process of the present invention, but do not limit the invention in any way.
The black fruit gland rib of embodiment 1 spends the hardening domestication of autumn tissue-cultured seedling greenhouse
(1) hardening greenhouse prepares：Ground point selection company flower planting glass attached-greenhouse, external sunshade net index of refraction is
75%, internal sunshade net index of refraction is 45%, preparing seedbed, the 300 times of carbendazim solution sterilizations in seedbed and surrounding sprinkling；
(2) hardening is managed before tissue-cultured seedling bottle outlet：Select neat and consistent of taking root, the black fruit gland rib of robust growth spends autumn tissue culture
Seedling, tissue culture bottle where the part seedling is neatly placed on attached-greenhouse seedbed, opens inside and outside sunshade net, and the built on seedbed
One layer of sunshade net of lid, makes intensity of illumination control in the range of 3000-4000lx, temperature range is controlled in 24-28 DEG C, humidity 75%
After left and right, hardening 3d, tissue culture bottle cap is turned on but not taken down, continue hardening 4d；
(3) cultivation matrix prepares with container：Using turfy soil and perlite, uniformly mixing is used as cultivation matrix, turf and pearl
Rock volume ratio V turfs：V perlite=1:1, black fruit gland rib spends autumn tissue-cultured seedling average height about 5cm, from 21 hole disks (3 ×
7), matrix is loaded, it is floating, with 1000 times of carbendazim solution spray disinfectants, select the special white clear nutrition of 1.5 cun of irises
Alms bowl (the high * bottoms footpath=4.8*4.8*3 of bore *, recycling), is sterilized with 1000 times of carbendazim solutions；
(4) tissue culture transplantation of seedlings：The tissue-cultured seedling that step (2) will be obtained is taken out with tweezers, with clear on the premise of root is not hindered
Water rinses culture medium well, and tissue-cultured seedling root is put into 1000 times of carbendazim solutions and soaks 2min, takes out after somewhat airing
It is colonized among hole tray 2, notices that root system is unfolded, matrix is compacted, pour permeable with spray mode, plant base portion exposes matrix about
0.5cm, matrix tips upside down on 1.5 cun of white clear nutritive cubes 1 in the hole tray after field planting away from edge about 1cm in hole tray；
(5) management is tamed after transplanting：
Water management：The warm indoor environment humidity of control is formed relatively between 75% or so, hole tray and the nutrition cup of back-off
Stable subenvironment, without water spray during domestication；
Illumination management：After transplanting before 15d, by inside and outside greenhouse sunshade net use control light intensity 5000-6000lx it
Between, the domestication of external sunshade net is opened afterwards；
Temperature treatment：Temperature control is between 24-28 DEG C in greenhouse；
(6) black fruit gland rib, which is spent, tames the time for 45d after autumn tissue culture transplantation of seedlings, afterwards remove the transparent nutritive cube of back-off,
Water management is changed to regular water spray, you can reach the requirement for changing basin production nutrition pot seedling or direct field-transplanting；
(7) black fruit gland rib spends autumn tissue-cultured seedling survival rate after hardening of the present invention domestication to be up to 97%, is survived than conventional hardening
Rate improves about 10% (research of high bright China etc., Black Box Tracing tissue-cultured seedling culture of rootage and domestication, northern gardening 2013
(09)：105-108), after hardening aerial growth amount than contrast test (unused white clear nutritive cube back-off, water management
Periodically water spray is changed to, other controls are consistent) improve about 50%.
The black fruit fructus lycii tissue-cultured seedling greenhouse hardening domestication of embodiment 2
(1) hardening greenhouse prepares：Ground point selection is same to be sprayed in glass attached-greenhouse described in embodiment 1, seedbed and surrounding
400 times of carbendazim solution sterilizations；
(2) hardening is managed before tissue-cultured seedling bottle outlet：The black fruit fructus lycii tissue-cultured seedling of take root at least 2 and neat and consistent are selected, will
Tissue culture bottle where the part seedling is neatly placed on attached-greenhouse seedbed, opens inside and outside sunshade net, and the built lid one on seedbed
Layer sunshade net, makes intensity of illumination control exist, in the range of 4000-5000lx, and temperature range control is at 18-24 DEG C, humidity about 70%,
After hardening 2d, tissue culture bottle cap is turned on but not taken down, continue hardening 5d；
(3) cultivation matrix prepares with container：Using turfy soil and perlite, uniformly mixing is used as cultivation matrix, turf and pearl
Rock volume ratio V turfs：V perlite=5:4, black fruit fructus lycii tissue-cultured seedling average height about 7cm, from 21 (3 × 7) hole disks, are filled out
Matrix is filled, it is floating, with 1000 times of carbendazim solution spray disinfectants, select the special white clear nutritive cube (mouth of 1.8 cun of irises
The high * bottoms footpath=4.8*4.8*3 of footpath *), with 1000 times of carbendazim solution soaking disinfections；
(4) tissue culture transplantation of seedlings：Tissue-cultured seedling is taken out with tweezers, rinsed well culture medium with clear water, notes protecting tissue culture
Seedling root is preserved from, and tissue-cultured seedling root is put into 1000 times of carbendazim solutions and soaks 2min, is taken out and is colonized after somewhat airing
Among hole tray 2, notice that root system is unfolded, matrix is compacted, pour permeable with spray mode, plant base portion exposes matrix about 1cm, base
Matter tips upside down on 1.8 cun of white clear nutritive cubes 1 in the hole tray after field planting away from edge about 1cm in hole tray；
(5) management is tamed after transplanting：
Water management：The warm indoor environment humidity of control is formed relatively between 70% or so, hole tray and the nutrition cup of back-off
Stable subenvironment, without water spray during domestication；
Illumination management：After transplanting before 10d, by inside and outside greenhouse sunshade net use control light intensity 7000-8000lx it
Between, the domestication of external sunshade net is opened afterwards；
Temperature treatment：Temperature control is between 18-24 DEG C in greenhouse；
(6) tame 35d after the transplantation of seedlings of black fruit fructus lycii tissue culture, afterwards remove the transparent nutritive cube of back-off, moisture, temperature,
Illumination management refers to normal greenhouse administrative skill, you can reach the requirement for changing basin production nutrition pot seedling or direct field-transplanting；
(7) survival rate is (unused whiter than contrast test to 100% after black fruit fructus lycii tissue-cultured seedling is tamed through hardening of the present invention
The transparent nutritive cube back-off of color, water management is changed to regular water spray, and other controls are consistent) improve about 15%.After hardening on the ground
Some growth amount is than contrast test increase about 30%.
The elder tissue-cultured seedling greenhouse hardening domestication of embodiment 3
(1) hardening greenhouse prepares：Ground point selection is in glass attached-greenhouse described in embodiment 1,2, seedbed and surrounding sprinkling 500
The sterilization of times carbendazim solution；
(2) hardening is managed before tissue-cultured seedling bottle outlet：The elder tissue-cultured seedling of robust growth, neat and consistent of taking root is selected, by this
Tissue culture bottle where the seedling of part is neatly placed on attached-greenhouse seedbed, opens inside and outside sunshade net, and one layer of built lid on seedbed
Sunshade net, makes intensity of illumination control exist, in the range of 3500-4500lx, and temperature range control is at 25-30 DEG C, humidity 60-70%,
After hardening 1d, tissue culture bottle cap is turned on but not taken down, continue hardening 6d；
(3) cultivation matrix prepares with container：Using turfy soil and perlite, uniformly mixing is used as cultivation matrix, turf and pearl
Rock volume ratio V turfs：V perlite=3:2, elder tissue-cultured seedling average height reaches 10cm, from 15 (3 × 5) hole disks, fills out
Matrix is filled, it is floating, with 1000 times of carbendazim solution spray disinfectants, select the special white clear nutritive cube (mouth of 2.5 cun of irises
The high * bottoms footpath=7.5*8*5 of footpath *), with 1000 times of carbendazim solution soaking disinfections；
(4) tissue culture transplantation of seedlings：Tissue-cultured seedling is taken out with tweezers, rinsed well culture medium with clear water, notes protecting tissue culture
Seedling root preserves from, and by tissue-cultured seedling root with 1000 times of carbendazim solution soaking disinfection 2min, takes out and is colonized after somewhat airing
Among hole tray 2, notice that root system is unfolded, matrix be compacted, pour permeable with spray mode, plant base portion exposes matrix about 0.5cm,
Matrix tips upside down on 2.5 cun of white clear nutritive cubes 1 in the hole tray after field planting away from edge about 2cm in hole tray；
(5) management is tamed after transplanting：
Water management：The warm indoor environment humidity of control is formed in the range of 60-70% between hole tray and the nutrition cup of back-off
Metastable subenvironment, without water spray during domestication；
Illumination management：After transplanting before 7d, by inside and outside greenhouse sunshade net use control light intensity 6500-7500lx it
Between, the domestication of external sunshade net is opened afterwards；
Temperature treatment：Temperature control is between 25-30 DEG C in greenhouse；
(6) 25d is tamed after the transplantation of seedlings of elder tissue culture, afterwards removed the transparent nutritive cube of back-off, moisture, temperature, light
Management refers to normal greenhouse administrative skill, you can reach the requirement for changing basin production nutrition pot seedling or direct field-transplanting；
(7) survival rate is to 100% after elder tissue-cultured seedling is tamed through hardening of the present invention, aerial growth amount after hardening
Improved than contrast test (unused white clear nutritive cube back-off, water management is changed to regular water spray, and other controls are consistent)
About 40%.
The pumila tissue-cultured seedling greenhouse hardening domestication of embodiment 4
(1) hardening greenhouse prepares：The same previous embodiment in place, the 300 times of carbendazim solution sterilizations in seedbed and surrounding sprinkling；
(2) hardening is managed before tissue-cultured seedling bottle outlet：The pumila tissue-cultured seedling of robust growth, neat and consistent of taking root is selected, by this
Tissue culture bottle where the seedling of part is neatly placed on attached-greenhouse seedbed, opens inside and outside sunshade net, and one layer of built lid on seedbed
Sunshade net, makes intensity of illumination control exist, in the range of 3000-3500lx, and temperature range control is in 22-28 DEG C, humidity about 75%, refining
After seedling 2d, tissue culture bottle cap is turned on but not taken down, continue hardening 5d；
(3) cultivation matrix prepares with container：Using turfy soil and perlite, uniformly mixing is used as cultivation matrix, turf and pearl
Rock volume ratio V turfs：V perlite=1:1, pumila tissue-cultured seedling average height reaches 7cm, and hole tray, nutrition cup are selected and sterilized
Handle the domestication of the black fruit fructus lycii training tissue culture seedling of be the same as Example 2；
(4) tissue culture transplantation of seedlings：Tissue-cultured seedling is taken out with tweezers, rinsed well culture medium with clear water, notes protecting tissue culture
Seedling root preserves from, and by tissue-cultured seedling root with 1000 times of carbendazim solution soaking disinfection 5min, takes out and is colonized after somewhat airing
Among hole tray 2, notice that root system is unfolded, matrix be compacted, pour permeable with spray mode, poured after water matrix away from hole tray along about
2cm, plant base portion exposes about 1cm slightly, and 1.8 cun of white clear nutritive cubes 1 are tipped upside down in the hole tray after field planting；
(5) management is tamed after transplanting：
Water management：The warm indoor environment humidity of control is formed relatively between 75% or so, hole tray and the nutrition cup of back-off
Stable subenvironment, without water spray during domestication；
Illumination management：After transplanting before 15d, by inside and outside greenhouse sunshade net use control light intensity 5500-6500lx it
Between, the domestication of external sunshade net is opened afterwards；
Temperature treatment：Temperature control is between 22-28 DEG C in greenhouse；
(6) 35d is tamed after the transplantation of seedlings of pumila tissue culture, afterwards removed the transparent nutritive cube of back-off, moisture, temperature, light
Management refers to normal greenhouse administrative skill, you can reach the requirement for changing basin production nutrition pot seedling or direct field-transplanting；
(7) survival rate is to 98% after pumila tissue-cultured seedling is tamed through hardening of the present invention, aerial growth amount after hardening
Improved than contrast test (unused white clear nutritive cube back-off, water management is changed to regular water spray, and other controls are consistent)
About 20%, survival rate improves about 15%.
The hall crabapple flower tissue-cultured seedling greenhouse hardening domestication of embodiment 5
(1) hardening greenhouse prepares：The same previous embodiment in place, the 300 times of carbendazim solution sterilizations in seedbed and surrounding sprinkling；
(2) hardening is managed before tissue-cultured seedling bottle outlet：The hall crabapple flower tissue-cultured seedling of robust growth, neat and consistent of taking root is selected, will
Tissue culture bottle where the part seedling is neatly placed on attached-greenhouse seedbed, opens inside and outside sunshade net, and the built lid one on seedbed
Layer sunshade net, makes intensity of illumination control exist, and in the range of 4500-5000lx, temperature range is controlled in 24-30 DEG C, humidity 75-
After 80%, hardening 3d, tissue culture bottle cap is turned on but not taken down, continue hardening 4d；
(3) cultivation matrix prepares with container：Cultivation matrix turf and perlite volume ratio V turfs：V perlite=3:2, hang down
Silk Malus spectabilis tissue-cultured seedling average height is about 5cm, and hole tray, nutrition cup, which are selected and disinfect the black fruit gland rib of be the same as Example 1, spends autumn group
Train the domestication of seedling hardening；
(4) tissue culture transplantation of seedlings：Tissue-cultured seedling is taken out with tweezers, rinsed well culture medium with clear water, notes protecting tissue culture
Seedling root preserves from, and by tissue-cultured seedling root with 1000 times of carbendazim solution soaking disinfection 5min, takes out and is colonized after somewhat airing
Among hole tray 2, notice that root system is unfolded, matrix be compacted, pour permeable with spray mode, poured after water matrix away from hole tray along about
1.5cm, plant base portion exposes about 0.5cm slightly, and 1.5 cun of white clear nutritive cubes 1 are tipped upside down in the hole tray after field planting；
(5) management is tamed after transplanting：
Water management：The warm indoor environment humidity of control is formed relatively between 80% or so, hole tray and the nutrition cup of back-off
Stable subenvironment, without water spray during domestication；
Illumination management：After transplanting before 10d, by inside and outside greenhouse sunshade net use control light intensity 7000-8000lx it
Between, the domestication of external sunshade net is opened afterwards；
Temperature treatment：Temperature control is between 24-30 DEG C in greenhouse；
(6) tame 45d after the transplantation of seedlings of hall crabapple flower tissue culture, afterwards remove the transparent nutritive cube of back-off, moisture, temperature,
Illumination management refers to normal greenhouse administrative skill, you can reach the requirement for changing basin production nutrition pot seedling or direct field-transplanting；
(7) survival rate is to 95% after hall crabapple flower tissue-cultured seedling is tamed through hardening of the present invention, and it is left that more conventional method improves 5%
After right (Zhang Lingling, hall crabapple flower tissue culture technique system, Xibei Univ. of Agricultural ＆ Forest Science ＆ Technology, Master's thesis, 2012), hardening on the ground
Than contrast test, (unused white clear nutritive cube back-off, water management is changed to regular water spray to some growth amount, and other controls are kept
About 20% unanimously) is improved, survival rate improves about 10%.
Claims (5)
1. a kind of method of Tissue Culture of Trees seedling greenhouse hardening domestication, it is characterised in that comprise the steps of：
(1) hardening greenhouse prepares：The conventional manageable glasshouse of epidemic disaster is selected, there is inside and outside sunshade net, seedbed is arranged,
Seedbed and surrounding environment sprinkling carbendazim solution sterilization；
(2) hardening is managed before tissue-cultured seedling bottle outlet：Robust plant, and the tissue-cultured seedling for neat and consistent of taking root are selected, by the tissue-cultured seedling institute
Moved in tissue culture bottle on hardening greenhouse seedbed, control is looked after after intensity, temperature, humidity, hardening 1-3d, and tissue culture bottle cap is turned on
But do not take down, continue hardening 4-6d；
(3) cultivation matrix prepares with container：The turfy soil and perlite of routine is selected to be used as cultivation matrix, turf and pearl rock mass
Product is than being 1:1~3:2, suitable hole tray is selected according to tissue-cultured seedling size, matrix is loaded, it is floating, with 800-1000 times of carbendazim
Spray solution is sterilized, and the special white clear nutritive cube of iris is selected according to hole tray size, is soaked with 1000 times of carbendazim solutions
Sterilization；
(4) tissue culture transplantation of seedlings：The tissue-cultured seedling that step (2) is obtained is taken out with tweezers, will be trained with clear water on the premise of root is not hindered
Foster base is rinsed well, and tissue-cultured seedling root is put into 1000 times of carbendazim solutions and soaks 2min, is colonized in after taking out somewhat airing
In hole tray after step (3) processing, root system is unfolded during field planting, and matrix is compacted, after compacting matrix away from hole tray along about 1-2cm,
Plant root does not expose, and base portion exposes 0.5-1cm, pours permeable with spray mode, by correspondingly sized white clear nutritive cube back-off
In hole tray after field planting；
(5) management is tamed after transplanting：
Water management：Warm indoor environment humidity is in the range of 60-80%；
Illumination management：7-15d before after transplanting, light intensity is controlled between 5000-8000lx by the use of sunshade net inside and outside greenhouse,
The domestication of external sunshade net is opened afterwards；
Temperature treatment：Different according to the forest species transplanted, warm indoor temperature general control is between 18-30 DEG C；
(6) the domestication time is generally 25-45d after transplanting, and afterwards removes the transparent nutritive cube of back-off, water management is changed to periodically
Water spray, you can reach the requirement for changing basin production nutrition pot seedling or direct field-transplanting.
2. the method for hardening domestication in Tissue Culture of Trees seedling greenhouse as claimed in claim 1, it is characterised in that many in step (1)
Bacterium spirit solution is 300-500 times of carbendazim solution.
3. the method for hardening domestication in Tissue Culture of Trees seedling greenhouse as claimed in claim 1, it is characterised in that described in step (2)
Intensity of illumination control in the range of 3000-5000lx, temperature be 18-30 DEG C, humidity 60-80%.
4. the method for hardening domestication in Tissue Culture of Trees seedling greenhouse as claimed in claim 1, it is characterised in that described in step (3)
Hole tray be 15 holes, 21 holes, 32 holes, 50 hole disks.
5. the method for hardening domestication in Tissue Culture of Trees seedling greenhouse as claimed in claim 1, it is characterised in that described in step (3)
's
The special white clear nutritive cube specification of iris is 1.5 cun~2.5 cun.
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| CN115211359A (en) * | 2022-08-22 | 2022-10-21 | 中国林业科学研究院林业研究所 | Seedling hardening method for tissue culture seedlings of poplar |
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