CN101313661A - Multitudinous transplant method for woody flower group cultivated seedling - Google Patents
Multitudinous transplant method for woody flower group cultivated seedling Download PDFInfo
- Publication number
- CN101313661A CN101313661A CNA2007100350274A CN200710035027A CN101313661A CN 101313661 A CN101313661 A CN 101313661A CN A2007100350274 A CNA2007100350274 A CN A2007100350274A CN 200710035027 A CN200710035027 A CN 200710035027A CN 101313661 A CN101313661 A CN 101313661A
- Authority
- CN
- China
- Prior art keywords
- container
- transplanting
- matrix
- transplant
- seedling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a planting method for culturing test-tube seedlings by plant tissues, in particular to the mass transplanting technique for tissue cultured seedlings of woody flowers. The invention adopts a container which is different from conventional culture flasks for one-time transplanting. The structure of the container used for transplanting is like a cup without a handle, and the bottom of the cup is provided with at least one hole, the mouth of the cup is provided with a cover, and the cover and the upper part of the container are transparent. The transplanting method using the container comprises the following steps that: substrates are prepared, the substrates are sterilized and filled in the container; the tissue cultured seedlings are sterilized; the tissue cultured seedlings are transplanted; the substrates in which the tissue cultured seedlings are planted are drenched by thimerosal; and the mouth of the container is sealed by the cover. Due to the adoption of the method, the seedling raising time is shortened, the transplanting survival rate can be kept at more than 90 percent, and the manufacturing cost is saved by 30 percent; moreover, the method is also wide in application.
Description
Technical field
The present invention relates to the cultivation method of Plant Tissue Breeding test-tube plantlet, specifically woody flower group cultivated seedling multitudinous transplant technology.
Background technology
Plant Tissue Breeding has been widely used in the seedling production of forest, flowers and other crops as a kind of means of breeding fast.Can widely applying of the tissue cultivating seedling of plant in-vitro propagate obtain good benefit, and the transplanting of tissue cultured test-tube seedling is crucial, transplanting survival rate and survive quality and directly influence the nursery stock production cost.
In the prior art, during the cultivation in bottle of the normal blake bottle that adopts in the first generation of Plant Tissue Breeding, subculture, the process of rooting culture, tissue cultivating seedling, be in all the time under high humidity, constant temperature, the low light environment, cause tissue cultivating seedling that following characteristics are arranged: the children is tender, loosely organized for tissue, and mechanical damage takes place easily; Blade stomatal closure performance imperfection, leaf table protective tissue is undeveloped, easily is desiccation; Undeveloped and root of root system and conducting tissue are obstructed, cause the transport capacity of water and nutrient relatively poor.And compare with herbage flower, many proterties characteristics of Woody flower are complicated and unstable, it is higher that its tissue culture technology requires, it is bigger to grasp difficulty, and be difficult for surviving after transplanting or survival rate on the low side, cause production cost higher.Based on These characteristics, traditional tissue cultivating seedling transplanting method must be transplanted through hardening, bottle outlet, container is transplanted three process, just can guarantee the tissue cultivating seedling transplanting survival rate, but its complex operation, the production cost height has limited its extensive utilization.
Many improvement of the traditional group of Woody flower being trained the transplantation of seedlings method are also disclosed in the prior art.Sun Zhenyuan etc.
[1]Invented korean rhododendron tissue cultivating seedling domestication transplantation technique, tissue cultivating seedling carries out water planting earlier before transplanting tamed about January, then was transplanted to flowerpot, and through the cultivation in January, survival rate is higher.Ceng Songjun etc.
[2]With the venezuela heliconia test-tube plantlet, transfer to and practice seedling under the natural daylight after 10 days, be transplanted to peat and add in the mixed-matrix of river sand, keep suitably ventilating and enough humidity, survival rate can reach 90%.Zhang Cunxu etc.
[3]Select fragrant osmanthus test-tube plantlet to put into greenhouse 2 weeks of domestication, transplanting is placed under the plastic greenhouse, opens plastic greenhouse after two weeks gradually, and continued growth is one month in the greenhouse, is colonizated in the land for growing field crops again, and survival rate is 93%.Li Qingwei etc.
[4]After the test-tube plantlet of photinia glabra grows 2～3 roots, put into greenhouse by solar heat, open the bottleneck hardening.Transplant after 8 days, cover film, heat and moisture preserving, survival rate is 91%.The big device of Wei etc.
[5]With Radix zanthoxyli rooting tube plantlet about 20 days at the natural daylight lower refining seedling, transplant on the seedbed of sterilizing when waiting height of seedling about 2 centimetres and degree of lignification higher, water permeablely, keep certain temperature and humidity, survival rate reaches 90%.Yao Lili etc.
[6]Discover that the Siberia Sorbus alnifloria seedling by the tissue-culturing rapid propagation approach is cultivated has only by transition hardening in laboratory, outdoor plastic canopy hardening, could improve survival rate.Tissue cultivating seedling survival rate by the transition hardening reaches more than 85%.Gu Honghui etc.
[7]Take the tissue cultivating seedling of the fast numerous Chinese azalea of method of outside sprout-cultivating-bottle, choose the subculture tissue cultivating seedling and be implanted into matrix after about 1 week of room scattering optical condition lower refining seedling, have root system to form after about 1 month, the tissue cultivating seedling survival rate of taking root is about 75%.Report that in addition tissue cultivating seedling sprays antitranspirant after transplanting at once, can significantly suppress tissue cultivating seedling blade pore and open a business, and then reduce transpiration rate, improve transplanting survival rate
[8]
The document that more than retrieves shows that traditional woody flower group cultivated seedling transplanting method has three process: hardening, bottle outlet are transplanted, container is transplanted.Many, the trivial operations of its operation causes tissue cultivating seedling very easily to be lowered into motility rate because of dehydration when transplanting, also taken more artificial, thereby cause the production cost of tissue cultivating seedling higher, profit is low.Just because of woody flower group cultivated seedling is transplanted difficulty, people are more to the transplantation technique research of tissue cultivating seedling at present, also obtained certain progress, but its research often rests on breadboard conceptual phase, or be confined to the production scale of small lot, and incompatible large-scale production need, and the survival rate of multitudinous transplant has only 60%～75%, realizes that really the woody flower group cultivated seedling industrialization produces seldom.So far do not see relevant woody flower group cultivated seedling multitudinous transplant survival rate up to more than 90%, and low-cost, be applicable to the report of the efficient tissue cultivating seedling transplantation technique of numerous Woody flower kinds.If can employ new technology, simplify tissue cultivating seedling and transplant operation, improve the tissue cultivating seedling transplanting survival rate, reduce production costs, improve tissue cultivating seedling production economic benefit, undoubtedly great facilitation will be arranged to the development of woody flower group cultivated fast numerous industry.
Summary of the invention
The object of the invention provides a kind of simple, survival rate tissue cultivating seedling multitudinous transplant method high, applied widely.
Adopt a kind of container that is different from traditional blake bottle to carry out disposable transplanting among the present invention.The structure of the container that this is used to transplant is the cup as no handle, and in the bottom at least one hole is arranged, and rim of a cup has covering; The top of this covering and container is printing opacity.
The method that adopts said vesse to transplant is as follows:
1) prepares matrix, comprise in the container of packing into after the sterilization matrix;
2) sterilization tissue cultivating seedling;
3) transplant tissue cultivating seedling;
4) drench with thimerosal and planted the matrix of tissue cultivating seedling;
5) seal, covering is sealed vessel port.
The container that is used to transplant described in the present invention can use existing disposal plastic cup, and beats some apertures at the bottom of cup, helps moisture penetration.
Described matrix can be selected according to different cultivated plants, matrix that also can preferred generic.
A kind of matrix of wide accommodation is provided in the embodiment of the invention, and the consist of yellow soil, charing rice husk ash, fine sand of this matrix mixed in 1: 2: 2 by volume.
Sterilization method can adopt the sterilization method that uses in the existing cultivation method among the present invention.Preferred 58% metalaxyl-mancozeb wettable powder in the embodiment of the invention: water is that 1: 1000 solution of weight ratio is as thimerosal.Sterilization is watered pouring matrix with thimerosal during matrix, and is wetting and do not tie plate for suitable with matrix.When disinfecting tissue cultivating seedling, use medicining liquid dipping 3 minutes.
The transplanting method that uses among the present invention can adopt the transplanting method that uses in the existing cultivation method.
The covering that uses on the transplanting container among the present invention can the use group be trained discarded film, saves cost, and can use repeatedly.
Traditional woody flower group cultivated seedling transplanting method has three process: hardening, bottle outlet are transplanted, container is transplanted; The present invention combines three process together, has shortened the one-tenth seedling time, and transplanting survival rate can be stabilized in more than 90%.Thereby reduced labour cost, also saved production cost 30%, improved the economic benefit that tissue cultivating seedling is produced significantly.And the present invention almost is applicable to all Woody flower kinds, need not restudy, and saves reasearch funds.
Embodiment
Embodiment 1
The tissue cultivating seedling of largeflower-like honeysuckle flower new varieties ' silver-colored emerald green flower bud ' is transplanted
Choose culture of rootage about 25 days, largeflower-like honeysuckle flower tissue cultivating seedling about up to 3 centimetres, adopt this new technology, without hardening, directly from blake bottle, take out test-tube plantlet, clean the root medium, in 0.1% (weight) metalaxyl-MnZn (58% wetting powder) solution, soaked 3 minutes, plant then in the plastic cup that fills yellow soil, charing rice husk ash, fine sand (1: 2: 2) mixed-matrix.With 0.1% (weight) metalaxyl solution matrix of drenching, seal and preserve moisture with the discarded film that seals of group training again, place the plastic tunnel of auto spraying, after 30 days, open and seal film, move into the land for growing field crops after 40 days.Transplant tissue cultivating seedling 3.0 ten thousand strains in March, 2006 altogether, statistics after 2 months, and survival rate reaches 94.8%.
Embodiment 2
Haw gland rib Sorbus alnifloria tissue cultivating seedling is transplanted
According to method of operating shown in the embodiment 1, transplant 5.0 ten thousand strains of haw gland rib Sorbus alnifloria tissue cultivating seedling in April, 2006, statistics after 2 months, survival rate reaches 99.6%.
Embodiment 3
The white holly tissue cultivating seedling is transplanted
According to method of operating shown in the embodiment 1, transplant 2.0 ten thousand strains of white holly tissue cultivating seedling in July, 2006, statistics after 2 months, and survival rate reaches 95.8%.
List of references:
[1] a kind of korean rhododendron tissue-culturing rapid propagation and transplanting method. application (patent right) people: Forestry Institue, Chinese Inst.of Forestry Sciences. open (bulletin) number: CN1732759; Open (bulletin) day: 2006.02.15.
[2] tissue culture propagation of venezuela heliconia. application (patent right) people: South-China Inst. of Botany, Chinese Academy of Sciences. open (bulletin) number: CN1442045; Open (bulletin) day: 2003.09.17
[3] Cinnamomum subavenium tissue culture and quick reproduction method. application (patent right) people: chongqing Xidun Industry Co. Ltd.. open (bulletin) number: CN1281637; Open (bulletin) day: 2001.01.31
[4] Li Qingwei, Sun Xinzheng, Chen Shichang etc. the callus induction of the red lip of photinia glabra and the research of plant regeneration method. Zhejiang agricultural journal [J], 2006,18 (4): 264-267.
[5] Wei Daqi, Wu Hongying, He Gui is whole etc. tissue culture of Radix zanthoxyli and breeding fast. Plant Physiology Communications [J], 2006,42 (1) 73.
[6] Yao Lili, Zhao Haihong, neutral battalion etc. the inferior Sorbus alnifloria training tissue culture seedling of two Berlis technology. water and soil conservation application technology [J], 2006,3:59-60.
[7] Gu Honghui, Yuan Qunying, Zhu Chunyan etc. tissue culture of Chinese azalea and breeding fast. Plant Physiology Communications [J], 2006 (42) 4:683.
[8] Shi Suen. the application study of antitranspirant in the apple test-tube seedling transplanting. Agricultural University Of Hebei's journal [J], 1994,17 (3): 1-4.
Claims (8)
1, be used to the container of transplanting, have the structure as common no handle cup, and in the bottom at least one hole is arranged, rim of a cup has covering; The top of this covering and container is printing opacity.
2, a kind of multitudinous transplant method for woody flower group cultivated seedling is characterized in that adopting the described container of claim 1 to transplant, and method is as follows:
1) prepares matrix, comprise in the container of packing into after the sterilization matrix;
2) sterilization tissue cultivating seedling;
3) transplant tissue cultivating seedling;
4) drench with thimerosal and planted the matrix of tissue cultivating seedling
5) seal, covering is sealed vessel port.
3, transplanting method according to claim 2 is characterized in that the employed container that is used to transplant, and is disposal plastic cup, and can uses repeatedly.
4, transplanting method according to claim 2 is characterized in that the consist of yellow soil, charing rice husk ash, fine sand of employed matrix mixed in 1: 2: 2 by volume.
5, transplanting method according to claim 2, it is characterized in that employed disinfectant adopts 58% metalaxyl mancozeb wettable powder: water is 1: 1000 solution of weight ratio.
6, transplanting method according to claim 2 when it is characterized in that sterilizing matrix, waters pouring matrix with thimerosal, and is wetting and do not tie plate for suitable with matrix.
7, transplanting method according to claim 2 when it is characterized in that disinfecting tissue cultivating seedling, is used medicining liquid dipping 3 minutes.
8, transplant largeflower-like honeysuckle flower, haw gland rib Sorbus alnifloria or white holly with the method for one of claim 2-7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100350274A CN101313661A (en) | 2007-05-30 | 2007-05-30 | Multitudinous transplant method for woody flower group cultivated seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100350274A CN101313661A (en) | 2007-05-30 | 2007-05-30 | Multitudinous transplant method for woody flower group cultivated seedling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101313661A true CN101313661A (en) | 2008-12-03 |
Family
ID=40104918
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100350274A Pending CN101313661A (en) | 2007-05-30 | 2007-05-30 | Multitudinous transplant method for woody flower group cultivated seedling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101313661A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102017896A (en) * | 2010-11-15 | 2011-04-20 | 江苏省·中国科学院植物研究所 | Method for quickly breeding Ilex dabieshanensis K Yao.et M.P.Deng in vitro |
| CN103563745A (en) * | 2013-10-12 | 2014-02-12 | 杭州市园林绿化股份有限公司 | Tissue culture method of ilex verticillata |
| CN103814736A (en) * | 2014-03-07 | 2014-05-28 | 安徽理工大学 | Cuckoo greenhouse seedling thinning transplantation method |
| CN103975832A (en) * | 2014-01-28 | 2014-08-13 | 华南农业大学 | Out-of-tube rooting method for tissue culture seedlings of silver birch |
| CN107155843A (en) * | 2017-05-16 | 2017-09-15 | 江苏省农业科学院 | A kind of method that the seedling industrialized transplanting of Chinese yam tissue culture and potato seed of improvement are cultivated |
-
2007
- 2007-05-30 CN CNA2007100350274A patent/CN101313661A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102017896A (en) * | 2010-11-15 | 2011-04-20 | 江苏省·中国科学院植物研究所 | Method for quickly breeding Ilex dabieshanensis K Yao.et M.P.Deng in vitro |
| CN103563745A (en) * | 2013-10-12 | 2014-02-12 | 杭州市园林绿化股份有限公司 | Tissue culture method of ilex verticillata |
| CN103563745B (en) * | 2013-10-12 | 2015-10-21 | 杭州市园林绿化股份有限公司 | A kind of method for tissue culture of ilex verticillata |
| CN103975832A (en) * | 2014-01-28 | 2014-08-13 | 华南农业大学 | Out-of-tube rooting method for tissue culture seedlings of silver birch |
| CN103975832B (en) * | 2014-01-28 | 2016-01-13 | 华南农业大学 | A kind of white wing silvery birch plantlet in vitro outside sprout-cultivating-bottle method |
| CN103814736A (en) * | 2014-03-07 | 2014-05-28 | 安徽理工大学 | Cuckoo greenhouse seedling thinning transplantation method |
| CN107155843A (en) * | 2017-05-16 | 2017-09-15 | 江苏省农业科学院 | A kind of method that the seedling industrialized transplanting of Chinese yam tissue culture and potato seed of improvement are cultivated |
| CN107155843B (en) * | 2017-05-16 | 2020-05-08 | 江苏省农业科学院 | Improved method for industrially transplanting tissue culture seedlings of Chinese yams and cultivating potato seeds |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101849506A (en) | Tissue culture and rapid propagation method of acer palmatum | |
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| CN103931492A (en) | Tissue-culture rapid seedling growing method for apple rootstock M9 | |
| CN101263787A (en) | Louis iris tissue culture fast seedling establishment and ecological application method | |
| CN103734009B (en) | Tissue culture method of anthurium | |
| CN101116424B (en) | Highly effective lily bulblet inducement culture method | |
| CN107018896A (en) | A kind of method of facility cuttage tilia miqueliana | |
| CN104396759B (en) | The method that ash tree tissue cultures is bred fast | |
| CN104303745A (en) | Fragrant plantain lily tissue culture seedling vitro-bottle rooting method | |
| CN104186317A (en) | Tissue culture rapid propagation method for Lagerstroemia intermedia Koehne | |
| CN102283090A (en) | Dryopteris erythrosora spore propagation method | |
| CN101313661A (en) | Multitudinous transplant method for woody flower group cultivated seedling | |
| CN103548660B (en) | Plant seedling pot allowing automatic liquid absorption | |
| CN107980531A (en) | A kind of method for transplanting of sugarcane tissue-culture nursery | |
| CN107197746A (en) | A kind of mating system of China fir field excellent resources | |
| CN104770173A (en) | High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings | |
| CN105145363B (en) | It is a kind of to significantly improve the method that China fir tissue culture produces emergence rate | |
| CN103283504B (en) | Method for grafting pear polyploidy test-tube plantlet outside test tube | |
| CN105532398B (en) | A kind of hosta tissue culture seedling Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method | |
| CN102524012B (en) | Exercising and transplanting method for fruit tree tissue culture seedlings | |
| CN111149674A (en) | Culture method for improving transplanting survival rate of plant tissue culture seedlings | |
| CN108651275A (en) | A kind of method of quickly breeding bletilla striata seedling under natural light | |
| CN103039362A (en) | Subculture medium for tissue culture seedling propagation of camellia oleifera abel and propagation method thereof | |
| CN110402818A (en) | A kind of method of excellent kind of Chinese chestnut Mature Embryo Tissue culture rapid seedling cultivation | |
| CN107466852B (en) | A kind of thick stem Japanese ardisia tissue cultures and rapid propagation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20081203 |