CN102283090A - Dryopteris erythrosora spore propagation method - Google Patents
Dryopteris erythrosora spore propagation method Download PDFInfo
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- CN102283090A CN102283090A CN201110174477A CN201110174477A CN102283090A CN 102283090 A CN102283090 A CN 102283090A CN 201110174477 A CN201110174477 A CN 201110174477A CN 201110174477 A CN201110174477 A CN 201110174477A CN 102283090 A CN102283090 A CN 102283090A
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- 238000000034 method Methods 0.000 title claims abstract description 26
- 241000703499 Dryopteris erythrosora Species 0.000 title abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 230000000366 juvenile effect Effects 0.000 claims abstract description 19
- 239000003415 peat Substances 0.000 claims abstract description 19
- 239000004576 sand Substances 0.000 claims abstract description 16
- 239000005357 flat glass Substances 0.000 claims abstract description 15
- 239000000725 suspension Substances 0.000 claims abstract description 15
- 230000008569 process Effects 0.000 claims abstract description 9
- 230000004720 fertilization Effects 0.000 claims abstract description 7
- 238000009331 sowing Methods 0.000 claims abstract description 5
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 239000011159 matrix material Substances 0.000 claims description 33
- 241001226158 Lastreopsis Species 0.000 claims description 31
- 230000007306 turnover Effects 0.000 claims description 14
- 235000019362 perlite Nutrition 0.000 claims description 9
- 239000010451 perlite Substances 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 7
- 230000035800 maturation Effects 0.000 claims description 6
- 230000004763 spore germination Effects 0.000 claims description 6
- 230000004083 survival effect Effects 0.000 abstract description 7
- 230000035784 germination Effects 0.000 abstract description 6
- 230000001954 sterilising effect Effects 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 2
- 229910001562 pearlite Inorganic materials 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 8
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 230000008030 elimination Effects 0.000 description 4
- 238000003379 elimination reaction Methods 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 241000167854 Bourreria succulenta Species 0.000 description 1
- 241000196133 Dryopteris Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- 229940039231 contrast media Drugs 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
Classifications
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- Y02P60/216—
Landscapes
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
A conventional Dryopteris erythrosora spore propagation method is mainly characterized in that Dryopteris erythrosora spores are prepared into suspension and the suspension is evenly poured onto mediums contained in an earthen pot by using a syringe; sowing mediums are mixtures of sand, peat and pearlite which are mixed by volume ratio of 1:1:1; the earthen pot into which the spores are sown is put into a transfer case, the height of the water-soaked bottom of the transfer case is 1.5cm and the water-soaked bottom of the transfer case is covered by plate glass; the spores are cultured in the sunshade position of a greenhouse at 25 DEG C to 35 DEG C under the condition that direct sunlight is avoided; juvenile sporophytes are obtained after culture and are respectively transplanted into transplanting mediums I which are formed by leaf mould, peat and sand in an evenly mixing way by volume ratio of 1:1:1, transplanting mediums II which are formed by sand, pearlite and peat in an evenly mixing way by volume ratio of 1:1:1, transplanting mediums III which are formed by peat and garden mould in a mixing way by volume ratio of 1:1, and reference mediums which are formed by garden mould; and the juvenile sporophytes are cultured under the same conditions. By using the characteristic that the germination and the fertilization of the spores need water and by applying pot planting, water soaking and moisture preservation to Dryopteris erythrosora spore propagation, the germination rate of the Dryopteris erythrosora spores, the seedling rate and the survival rate of the transplant sporophytes are effectively improved; in the propagation process, any sterilization process is not required, the processes are reduced, the pollution is reduced, the cost is saved, the operation is simple and convenient and the rapid propagation and the mass production of seedlings are facilitated; the survival rate of the transplanted juvenile spore seedlings reaches more than 85 percent.
Description
Technical field
The present invention relates to the sporogenesis method of pteridophyte, relate to the sporogenesis method of the red lid shield-fern of a kind of fast and convenient breeding specifically.
Background technology
Red lid shield-fern (Dryopteris erythrosora) is a Dryopteridaceae Dryopteris evergreen herbage, is medium-sized Lu Sheng fern.Be distributed widely in East China, south China and southwest, be born in acidity or the faintly acid soil, like dark and damp growing environment, regional distribution height above sea level is below 300m in Jiangsu.Red lid shield-fern rhizome is tiltedly given birth to, the blade collection stem top of taking root, and graceful elegant, plant type is attractive in appearance, and the leaf look dark green is glossy, and the cherry sporangiorus lid of blade back is beautiful in colour, and higher ornamental value is arranged, and is that pteridophyte is viewed and admired in rare evergreen, area, Nanjing.Under cultivation condition, red lid shield-fern begins to sprout the fist leaf roll at early March, and early April begins to open up leaf, sporangiorus occurs about April 5, beginning maturation about June 10, and along with the sprouting of new blade, the spore maturing stage can be extended to early November.Red lid shield-fern has higher shade tolerance, utilizes this characteristics, in dark corner or the bigger place of sylvan life crown density that other plant can't base oneself upon, plant the red lid shield-fern that is implanted with higher ornamental value, can form normal growth, the attitude grace covers the ground cover plant group on the face of land fast; Red lid shield-fern is to solve the very desirable ground cover plant of swale greening, can form the peculiar view that is different from other plant.Red lid shield-fern not only can utilize on the ground quilt, and also can be used as cut-flower flower material and use, also be a kind of medicinal plant simultaneously, red lid shield-fern is a pteridophyte that serves many purposes, and has higher popularization and application values.
Red lid shield-fern mainly rely on the breeding of spore to carry out the procreation of population, but natural propagation speed is slow under field conditions (factors), and reproduction coefficient is low, and wild resource can not satisfy the demand in development and use and market; Simultaneously, excessive collection wild resource can cause great destruction to ecotope, so be badly in need of and will manually breed in a large number, addresses the above problem.
Plurality of advantages such as sporogenesis is manually to carry out the main method that reproduction coefficient was bred and improved to extensive fern, and it has with low cost, and method is simple, and breeding amount is big.At present, also unmanned sporogenesis to red lid shield-fern routine carries out correlative study, does not also see the report of patent application.
Summary of the invention
It is less that the present invention is primarily aimed at red lid shield-fern resource, can not satisfy the Landscape Application demand, and a kind of quick, simple and easy to do red lid shield-fern sporogenesis method is provided.
The present invention adopts conventional spore seed propagation method, carries out the quick breeding of red lid shield-fern, can access a large amount of juvenile sporophytes, thereby realize purpose of the present invention.
The sporogenesis method of red lid shield-fern mainly contains following step:
(1) hot lid shield-fern sporangiorus transfers yellow to by redness, and indusium is opened, and when exposing the black spore, collects spore, and spore is placed in the container that is contained in clear water, stirs.
(2) draw the suspension that contains spore with syringe, sow equably on the matrix in earthen basin, suppress gently in the earthen basin stromal surface with the plank that circle is with handles, earthen basin is placed in the fluid-tight plastic box, water level keeps about 1.5cm at the bottom of the case, guarantee that matrix fully absorbs moisture, and guarantee spore germination and the requirement of fertilization to moisture, Turnover Box upper cover plate glass.Temperature keeps 25 ℃-35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.
(3) the earthen basin kind that has the juvenile sporophyte of 2-3 sheet leaf to be transplanted to the matrix for preparing length is cultivated, and the earthen basin temperature keeps 25 ℃-35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.Earthen basin is placed in the fluid-tight plastic box, and water level keeps about 1.5cm at the bottom of the case, the transplanting initial stage, cover plate glass on the Turnover Box, and open after the week,, carry out normal management with reference to anti-cloudy flowers seedling management flow process.
The matrix of sowing spore is the mixture of sand+peat+perlite (1: 1: 1, volume ratio).
1. transplanting medium is to be mixed by leaf mould+peat+sand (1: 1: 1, volume ratio); 2. transplanting medium is evenly to be mixed by sand+perlite+peat (1: 1: 1, volume ratio); 3. transplanting medium is mixed by peat+garden mould (1: 1, volume ratio); Contrast media is a garden mould.
The present invention adopts conventional sporogenesis method, and matrix and earthen basin are all less than sterilization; The method of this conventional seed planting spore is applied to the sporogenesis of red lid shield-fern, does not have disinfectant program, can save operation, reduces and pollutes, and saves cost, and easy and simple to handle, common peasant household can operate, and helps the fast breeding and the large-scale production of seedling.The transplanting survival rate of children's spore seedling reaches more than 85%.This The Application of Technology will provide technical guarantee for red lid shield-fern moves towards market.
Embodiment
Embodiment 1
When sporangiorus transfers yellow to by redness, indusium is opened, when exposing the black spore, the red lid shield-fern blade of spore maturation is adopted down, and court is placed down on the big blank sheet of paper vacuum side of blade, after treating 5 days, gently shake blade with have gentle hands, again blade is taken away,, installed standby with paper bag collecting good spore impurity elimination.The abundant mixing of matrix sand+peat+perlite (1: 1: 1, volume ratio); And spore is placed in the container that is contained in clear water, stir, make suspension; Draw the suspension that contains spore with syringe, water equably on the matrix in earthen basin, suppress gently in the earthen basin stromal surface with the plank that circle is with handles, spore is fully contacted with matrix, earthen basin is placed in the fluid-tight plastic box, and water level keeps about 1.5cm at the bottom of the case, guarantees that matrix fully absorbs moisture, and assurance spore germination and the requirement of fertilization to moisture, Turnover Box upper cover plate glass.Temperature keeps 25 ℃～35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.Sow after 6 days, spore begins sporoderm-broken germination, and prothallium begins to occur after 2 weeks, and after 70 days, juvenile sporophyte begins to occur, and after 120 days, sporophyte forms in a large number.
The long juvenile sporophyte that 2～3 blades are arranged was transplanted to leaf mould+peat with the earthen basin splendid attire+sand (1: 1: 1, volume ratio) on the matrix that mixes, earthen basin is placed in the fluid-tight plastic box, water level keeps about 1.5cm at the bottom of the case, initial stage, cover plate glass on the Turnover Box, open after the week, carry out normal management with reference to anti-cloudy flowers seedling management flow process.Temperature keeps 25 ℃～35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.The juvenile sporophyte survival rate reaches more than 85%.
Embodiment 2
When sporangiorus transfers yellow to by redness, indusium is opened, when exposing the black spore, the red lid shield-fern blade of spore maturation is adopted down, and court is placed down on the big blank sheet of paper vacuum side of blade, after treating 5 days, gently shake blade with have gentle hands, again blade is taken away,, installed standby with paper bag collecting good spore impurity elimination.The abundant mixing of matrix sand+peat+perlite (1: 1: 1, volume ratio), and spore is placed in the container that is contained in clear water, stirs, make suspension; Draw the suspension that contains spore with syringe, water equably on the matrix in earthen basin, suppress gently in the earthen basin stromal surface with the plank that circle is with handles, spore is fully contacted with matrix, earthen basin is placed in the fluid-tight plastic box, and water level keeps about 1.5cm at the bottom of the case, guarantees that matrix fully absorbs moisture, and assurance spore germination and the requirement of fertilization to moisture, Turnover Box upper cover plate glass.Temperature keeps 25 ℃～35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.Sow after 6 days, spore begins sporoderm-broken germination, and prothallium begins to occur after 2 weeks, and after 70 days, juvenile sporophyte begins to occur, and after 120 days, sporophyte forms in a large number.
The long juvenile sporophyte that 2-3 sheet blade arranged was transplanted to sand+perlite with the earthen basin splendid attire+peat (1: 1: 1, volume ratio) on the matrix that evenly mixes, earthen basin is placed in the fluid-tight plastic box, water level keeps about 1.5cm at the bottom of the case, initial stage, cover plate glass on the Turnover Box, open after the week, carry out normal management with reference to anti-cloudy flowers seedling management flow process.Temperature keeps 25 ℃～35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.The juvenile sporophyte survival rate reaches more than 80%.
Embodiment 3
When sporangiorus transfers yellow to by redness, indusium is opened, when exposing the black spore, the red lid shield-fern blade of spore maturation is adopted down, and court is placed down on the big blank sheet of paper vacuum side of blade, after treating 5 days, gently shake blade with have gentle hands, again blade is taken away,, installed standby with paper bag collecting good spore impurity elimination.The abundant mixing of matrix sand+peat+perlite (1: 1: 1, volume ratio), and spore is placed in the container that is contained in clear water, stirs, make suspension; Draw the suspension that contains spore with syringe, water equably on the matrix in earthen basin, suppress gently in the earthen basin stromal surface with the plank that circle is with handles, spore is fully contacted with matrix, earthen basin is placed in the fluid-tight plastic box, and water level keeps about 1.5cm at the bottom of the case, guarantees that matrix fully absorbs moisture, and assurance spore germination and the requirement of fertilization to moisture, Turnover Box upper cover plate glass.Temperature keeps 25 ℃～35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.Sow after 6 days, spore begins sporoderm-broken germination, and prothallium begins to occur after 2 weeks, and after 70 days, juvenile sporophyte begins to occur, and after 120 days, sporophyte forms in a large number.
The long juvenile sporophyte that 2～3 blades are arranged is transplanted to the peat+garden mould of earthen basin splendid attire (1: 1, volume ratio) on the matrix that evenly mixes, earthen basin is placed in the fluid-tight plastic box, water level keeps about 1.5cm at the bottom of the case, initial stage, cover plate glass on the Turnover Box, open after the week, carry out normal management with reference to anti-cloudy flowers seedling management flow process.Temperature keeps 25 ℃～35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.The juvenile sporophyte survival rate reaches more than 75%.
Embodiment 4 contrasts
When sporangiorus transfers yellow to by redness, indusium is opened, when exposing the black spore, the red lid shield-fern blade of spore maturation is adopted down, and court is placed down on the big blank sheet of paper vacuum side of blade, after treating 5 days, gently shake blade with have gentle hands, again blade is taken away,, installed standby with paper bag collecting good spore impurity elimination.The abundant mixing of matrix sand+peat+perlite (1: 1: 1, volume ratio), and spore is placed in the container that is contained in clear water, stirs, make suspension; Draw the suspension that contains spore with syringe, water equably on the matrix in earthen basin, suppress gently in the earthen basin stromal surface with the plank that circle is with handles, spore is fully contacted with matrix, earthen basin is placed in the fluid-tight plastic box, and water level keeps about 1.5cm at the bottom of the case, guarantees that matrix fully absorbs moisture, and assurance spore germination and the requirement of fertilization to moisture, Turnover Box upper cover plate glass.Broadcast temperature and keep 25 ℃～35 ℃, be placed on the place of giving shade in greenhouse, avoid the sunlight direct projection.Plant after 6 days, spore begins sporoderm-broken germination, and prothallium begin to occur after 2 weeks, and after 70 days, juvenile sporophyte begins to occur, and after 120 days, sporophyte forms in a large number.
There is the juvenile sporophyte of 2～3 blades to be transplanted on the garden mould matrix of using the earthen basin splendid attire with long, earthen basin is placed in the fluid-tight plastic box, water level keeps about 1.5cm at the bottom of the case, initial stage, cover plate glass on the Turnover Box, open after one week, carry out normal management with reference to anti-cloudy flowers seedling management flow process.Temperature keeps 25 ℃～35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.The juvenile sporophyte survival rate reaches about 40%.
Claims (5)
1. the sporogenesis method of a red lid shield-fern, its feature may further comprise the steps:
(1) spore of the red lid shield-fern of collection is placed on spore in the clear water, stirs, and makes the suspension that contains spore.
(2) draw the suspension that contains spore with syringe, water equably on the matrix in earthen basin, earthen basin is placed in the fluid-tight plastic box, and water level keeps about 1.5cm at the bottom of the case, Turnover Box upper cover plate glass.Temperature keeps 25 ℃～35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.
(3) have the juvenile sporophyte of 2-3 sheet leaf to be transplanted in the earthen basin for preparing matrix length and cultivate, earthen basin is placed in the fluid-tight plastic box, and water level keeps about 1.5cm at the bottom of the case, the initial stage, cover plate glass on the Turnover Box, and open normal management after the week.Cultivation temperature keeps 25 ℃～35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.
The matrix of sowing spore is the mixture of sand+peat+perlite (1: 1: 1, volume ratio).
Transplanting medium is to be mixed by leaf mould+peat+sand (1: 1: 1, volume ratio).
2. according to the sporogenesis method of right 1 described red lid shield-fern, it is characterized in that: hot lid shield-fern sporangiorus transfers yellow to by redness, indusium is opened, when exposing the black spore, the red lid shield-fern blade of spore maturation is adopted down, court is placed down on the big blank sheet of paper vacuum side of blade, after treating 5 days, gently shake blade with have gentle hands, again blade is taken away, collected spore; Spore is placed in the container that fills clear water, stirs and make the suspension that contains spore; Sowing media is the mixture of matrix sand+peat+perlite (1: 1: 1, volume ratio).
3. according to the sporogenesis method of right 1 described red lid shield-fern, it is characterized in that: draw the suspension that contains spore with syringe, water equably on the matrix in earthen basin, earthen basin is placed in the fluid-tight plastic box, water level keeps about 1.5cm at the bottom of the case, guarantee that matrix fully absorbs moisture, and guarantee spore germination and the requirement of fertilization to moisture, Turnover Box upper cover plate glass is preserved moisture; Spore, matrix and earthen basin all need not sterilized.
4. according to the sporogenesis method of right 1 described red lid shield-fern, it is characterized in that: temperature keeps 25 ℃～35 ℃, is placed on the place of giving shade in greenhouse, avoids the sunlight direct projection.
5. according to the sporogenesis method of right 1 described red lid shield-fern, it is characterized in that: the long juvenile sporophyte that 2～3 blades are arranged is transplanted on the matrix that the leaf mould+peat+sand (1: 1: 1, volume ratio) with the earthen basin splendid attire mixes, puts into Turnover Box, cover plate glass, preserve moisture a week; Open after one week, carry out normal management with reference to anti-cloudy flowers seedling management flow process, condition of culture is with sowing spore condition of culture.
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| CN201110174477A CN102283090A (en) | 2011-06-27 | 2011-06-27 | Dryopteris erythrosora spore propagation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110174477A CN102283090A (en) | 2011-06-27 | 2011-06-27 | Dryopteris erythrosora spore propagation method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860262A (en) * | 2012-10-25 | 2013-01-09 | 南京林业大学 | Propagation method of cyrtomium fortunei |
| CN103348846A (en) * | 2013-06-10 | 2013-10-16 | 哈尔滨师范大学 | Reproduction method of isoetes yunguiensis |
| CN103999750A (en) * | 2014-05-22 | 2014-08-27 | 丽水学院 | Method for breeding moonwort spores |
| CN104604493A (en) * | 2015-01-26 | 2015-05-13 | 河南科技学院 | Cyrtomium fortunei spore reproduction method |
| CN104904445A (en) * | 2014-03-14 | 2015-09-16 | 临安市高山珍稀药材培植研究所 | Cultivation method for ternate grape fern herb |
| CN106688842A (en) * | 2016-11-17 | 2017-05-24 | 中国长江三峡集团公司 | Seeding and propagating method of lotus leaf adiantum spore |
| CN106942052A (en) * | 2017-03-02 | 2017-07-14 | 东北农业大学 | The industrial seedling rearing and artificial cultivation method of a kind of dryopteris fragrans |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101297635A (en) * | 2008-06-30 | 2008-11-05 | 中国科学院华南植物园 | Method for breeding spore of Dryopteris varia |
-
2011
- 2011-06-27 CN CN201110174477A patent/CN102283090A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101297635A (en) * | 2008-06-30 | 2008-11-05 | 中国科学院华南植物园 | Method for breeding spore of Dryopteris varia |
Non-Patent Citations (3)
| Title |
|---|
| 《中国优秀硕士论文全文数据库农业科技辑》 20081231 吴芹 几种观赏蕨类植物的繁殖技术研究 第D048-149页 1-4 , 第2期 * |
| 《安徽农业科学》 20101231 孙起梦等 几种蕨类植物的孢子繁殖试验研究 第13571- 13572, 13576页 1-4 第38卷, 第25期 * |
| 《广西师范大学学报: 自然科学版》 20071231 罗顺元等 红盖鳞毛蕨配子体的发育 1-4 第25卷, 第3期 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860262A (en) * | 2012-10-25 | 2013-01-09 | 南京林业大学 | Propagation method of cyrtomium fortunei |
| CN102860262B (en) * | 2012-10-25 | 2013-07-10 | 南京林业大学 | Propagation method of cyrtomium fortunei |
| CN103348846A (en) * | 2013-06-10 | 2013-10-16 | 哈尔滨师范大学 | Reproduction method of isoetes yunguiensis |
| CN103348846B (en) * | 2013-06-10 | 2015-06-17 | 哈尔滨师范大学 | Reproduction method of isoetes yunguiensis |
| CN104904445A (en) * | 2014-03-14 | 2015-09-16 | 临安市高山珍稀药材培植研究所 | Cultivation method for ternate grape fern herb |
| CN103999750A (en) * | 2014-05-22 | 2014-08-27 | 丽水学院 | Method for breeding moonwort spores |
| CN104604493A (en) * | 2015-01-26 | 2015-05-13 | 河南科技学院 | Cyrtomium fortunei spore reproduction method |
| CN106688842A (en) * | 2016-11-17 | 2017-05-24 | 中国长江三峡集团公司 | Seeding and propagating method of lotus leaf adiantum spore |
| CN106942052A (en) * | 2017-03-02 | 2017-07-14 | 东北农业大学 | The industrial seedling rearing and artificial cultivation method of a kind of dryopteris fragrans |
| CN106942052B (en) * | 2017-03-02 | 2019-01-15 | 东北农业大学 | A kind of industrial seedling rearing and artificial cultivation method of dryopteris fragrans |
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Application publication date: 20111221 |