CN106688842A - Seeding and propagating method of lotus leaf adiantum spore - Google Patents
Seeding and propagating method of lotus leaf adiantum spore Download PDFInfo
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- CN106688842A CN106688842A CN201611021955.0A CN201611021955A CN106688842A CN 106688842 A CN106688842 A CN 106688842A CN 201611021955 A CN201611021955 A CN 201611021955A CN 106688842 A CN106688842 A CN 106688842A
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- spore
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Abstract
The invention discloses a seeding and propagating method of a lotus leaf adiantum spore, and belongs to the technical field of forest propagation. The main technical point is to use lotus leaf adiantum spore as a material, apply a series of measures such as selection and disinfection of seeding matrix, disinfection of sowing container and drug and cultivation environment of seeding and emergence periods, and moisture management, so as to promote the spore seeding and propagating efficiency. The seeding and propagating method of the lotus leaf adiantum spore is simple in method, short in seedling time, high in seedling rate, high in survival rate of transplanting, and can reduce the production cost, and is significant to protect national rare and endangered species.
Description
Technical field
The present invention relates to a kind of practical technique of Adiantum reniforme L var sinense Y. X. Lin spore seed propagation.
Background technology
Adiantum reniforme L var sinense Y. X. Lin also known as lotus leaf desmodium, iron wire grass, are the only one China endemic species under adiantaceae Adiantum.
Only it is distributed in the band of China Yangtze River Gorges one, height above sea level 80-430m.Adiantum reniforme L var sinense Y. X. Lin root-like stock is short and upright, single leafage life, leaf
Piece garden kidney shape, petiole brilliant black, compact uniqueness is available for viewing and admiring；Also there is important medical value, to jaundice type hepatitis, urinary system togetherness
The diseases such as dye, tympanitis have good therapeutic effect.But Adiantum reniforme L var sinense Y. X. Lin natural propagation ability is weak, rare numbers, Three Gorges Projects are built
The original producton location of below height above sea level 175m will be submerged afterwards, and separately by man's activities such as immigrant's activities, its living environment is seriously threatened.
It is now first-grade state protection wild plant, national two grades of rare extinction plants.
Wuhan plant institute of Chinese Academy of Sciences Xu Hui pearls exist《Yangtze river basin resource and environment》Deliver《The rare special product in reservoir area of Three Gorges is planted
The sporogenesis of thing Adiantum reniforme L var sinense Y. X. Lin》A kind of Adiantum reniforme L var sinense Y. X. Lin spore seed propagation method is disclosed, with high-quality leaf mould to broadcast
, there is first plant of sporinite seedling and needs 110 days from being seeded into kind of matrix, but the method tedious process, and seedling-growing time is more long, have into
One step simplifies operation and shortens the space of time；Institute Of Unity and Coherence In Writing Of Chongqing Zhang Zurong exists《Agriculture of Anhui science》Deliver《Spinulose tree fern with
The sporogenesis technical research of Adiantum reniforme L var sinense Y. X. Lin》In with primary border soil as sowing media, emergence rate is up to 46.6%, but this side
Method is relatively costly, and emergence rate has the space of further lifting.
The content of the invention
Instant invention overcomes deficiency of the prior art, by way of simplifying seeding method and adjustment sowing time management,
Easy to operate there is provided one kind, speed of emergence is fast, emergence rate method high.
The selection of experiment material
The spore of Adiantum reniforme L var sinense Y. X. Lin is just opened in mid or late June to maturation between early August, period when spore bag hole, spore
Band leaf harvesting in time is used as sowing material when not being scattered also；
Sowing media, container selection and treatment
By sowing media (1) leaf mould or (2) peat：Perlite is 3.5 in mass ratio：1 mixture or (3) river
Husky or (4) peat：River sand is 1 in mass ratio:(more preferably described sowing media is peat to 1 mixture：River sand
In mass ratio it is 1:1 mixture.)；The high-temperature sterilization 30min in high-temperature sterilization pot or dryer, rear overlay film is sudden and violent under the sun
Shine 3-4h standby；Sowing container is bore 13-17cm common plasticses flowerpots, with being cleaned after boiling water plus detergent immersion 20min, dried in the air
It is dry stand-by.
Adiantum reniforme L var sinense Y. X. Lin spore is sowed
Treated sowing media is loaded into flowerpot, is filled to away from basin mouthful 3-5cm, basin heelpiece crock or foam are easy to drip
Water, sowing media device pours permeable after finishing with the carbendazim solution for being configured to 800 times, by surfacing, removes surface impurity；
Prepare blank sheet of paper or filter paper, sporangiorus gently stirred with blade, allow spore to become scattered about on paper, after by scraps of paper doubling, spore is equal
It is even to trickle down in flowerpot surface, it is impossible to rewater；Preservative film is covered on flowerpot, the water spray moisturizing of preservative film inner side prepares several modelings
Material square plate, 3-5cm clear water is filled after cleaning, and the flowerpot that will broadcast spore is put into wherein；The square plate that flowerpot will be filled is positioned over greenhouse
Sowing time management is carried out in greenhouse or in outdoor bower or culturing room (more preferably in the treatment of outdoor bower).
Adiantum reniforme L var sinense Y. X. Lin spore sowing time management
Sowing is first 7 days, and clear water in square plate is exchanged daily, and adds 2000 times of carbendazim, to side spray in preservative film
Spill clear water moisturizing；In the 7-30 days, clear water in square plate was exchanged in every 3 days, to the sprinkling clear water moisturizing of preservative film inner side, and and
The liver moss and other floating materials of growth in Shi Qingxi square plates；Then clear water in square plate was exchanged in 5-7 days after 30 days, to preservative film
Clear water moisturizing is sprayed in inner side, the liver moss and other floating materials for being grown in cleaning square plate in time；After sporinite seedling is formed, by flower
Basin is taken out from square plate, removes the preservative film of covering, and a small amount of surface water is sprayed daily.
Seedling replanting
Transplanted into sowing media by when seedling sporinite grows 3 and above true leaf, transplanting container uses 64-72
Hole disk or bore 5cm common plasticses flowerpots, root system should not be destroyed when taking out seedling, and with certain sowing media；Transplant
After pour permeable, be put into maintenance in warmhouse booth, can be completed after 30d and survival rate can be counted.
The advantage of Adiantum reniforme L var sinense Y. X. Lin spore seed propagation practical technique
2.1 Adiantum reniforme L var sinense Y. X. Lin spore seed propagation practical and technical methods are easy, and sowing media high temperature insolation sterilization, sowing are held
The combined treatment of device high-temperature sterilization, carbendazim solution immersion and spray disinfectant greatly reduces the miscellaneous bacteria infection between insemination and emergence
Rate.
2.2 are processed by matrix immersion before sowing, can be prevented effectively from the spore water suction for causing that after planting rewaters it is excessive from
And the phenomenon such as dead or seedling vitrifying, emergence rate can be effectively improved and quality of emerging.
2.3 by sowing when culture environment selection and insemination and emergence phase preservative film covering spray water, plastics square plate be filled with water
The treatment combination for changing water cleaning improves sowing efficiency；When greatly improving insemination and emergence by the selection of sowing, transplanting medium
Between, emergence rate and transplanting survival rate.
2.4 this method experiment materials can be with adopting with broadcasting, it is not necessary to carries out any treatment, it is not required that refrigerated or husky
Hide, simplify operating process, reduce production cost.
Brief description of the drawings
Fig. 1 is formed for sporinite seedling.
Sporinite growth of seedling situation after 100d is sowed outside Fig. 2 rooms, left figure is warmhouse booth, and right figure is outdoor bower.
Growing state after Fig. 3 sporinite seedling replantings 60d.
Basin growing state after 1 year on Fig. 4 seedling replantings.
Specific embodiment
Embodiment 1
Adiantum reniforme L var sinense Y. X. Lin spore seed propagation practical technique
Experiment material
The spore of Adiantum reniforme L var sinense Y. X. Lin is just opened in mid or late June to maturation between early August, period when spore bag hole, spore
Band leaf harvesting in time is used as sowing material when not being scattered also.
1.2 sowing medias, container selection and treatment
Sowing media selects four kinds, (1) leaf mould；(2) peat：Perlite=3.5:1；(3) river sand；(4) peat：River sand
=1:1；Sowing media collect after in high-temperature sterilization pot or dryer high-temperature sterilization 30min, rear overlay film is exposed to the sun 3- under the sun
4h is standby；Sowing container is bore 13-17cm common plasticses flowerpots, with being cleaned after boiling water plus detergent immersion 20min, dry and treat
With.Time, first sporinite seedling formation time are formed with regard to gametophyte respectively in four kinds of Medium Cultures and first spore is grown
The average development of evil in febrile disease rates of 30d are counted after body seedling, the results are shown in Table one.
The Adiantum reniforme L var sinense Y. X. Lin spore Seeded growth situation in different substrates of table one
Peat as shown in Table 1：River sand=1:1 is optimal sowing media, and its gametophyte forms the time for 21d, first spore
Daughter seedling forms the time for 54d, and average conversion is 91% after sporinite seedling forms 30d, and it is in seedling-growing time and conversion
There is very big lifting in rate.
Adiantum reniforme L var sinense Y. X. Lin spore is sowed
(1) by treated peat：River sand=1:1 sowing media loads flowerpot, is filled to away from basin mouthful 3-5cm, and basin heelpiece is broken
Tile or foam pour permeable after being easy to draining, sowing media device to finish with the carbendazim solution for being configured to 800 times, and surface is put down
It is whole, remove surface impurity；(2) prepare blank sheet of paper or filter paper, sporangiorus is gently stirred with blade, allow spore to become scattered about on paper, after
By scraps of paper doubling, spore is uniformly trickled down in flowerpot surface, be not required to rewater；(3) preservative film is covered on flowerpot, in preservative film
Side spray water moisturizing.Prepare several plastics square plates, 3-5cm clear water is filled after cleaning, the flowerpot that will broadcast spore is put into wherein；(4) will
The square plate for filling flowerpot is positioned over 3 varying environments (in warmhouse booth, outdoor bower, in culturing room) and carries out sowing time pipe
Reason.Time, first sporinite seedling are formed in three kinds of culture environments with regard to gametophyte respectively to form the time and grow first
The average development of evil in febrile disease rates of 30d are counted after sporinite seedling, the results are shown in Table two.
Spore Seeded growth situation under the different culture environments of table two
As shown in Table 2, spore can form sporinite seedling under three kinds of environment, but 3 conversion ratios for the treatment of are too low, emerge
Time is oversize, and the best culture environment of effect is 1 warmhouse booth for the treatment of, but with bower outside treatment Room 2 without marked difference, from children
Seedling environmental suitability and it is cost-effective in general, the outer bower in treatment Room 2 is optimal selection.
Adiantum reniforme L var sinense Y. X. Lin spore sowing time management
Sowing is first 7 days, and clear water in square plate is exchanged daily, and adds 2000 times of carbendazim, to side spray in preservative film
Spill clear water moisturizing.In the 7-30 days, clear water in square plate was exchanged in every 3 days, to the sprinkling clear water moisturizing of preservative film inner side, and and
The liver moss and other floating materials of growth in Shi Qingxi square plates.Then clear water in square plate was exchanged in 5-7 days after 30 days, to preservative film
Clear water moisturizing is sprayed in inner side, the liver moss and other floating materials for being grown in cleaning square plate in time.After sporinite seedling is formed, by flower
Basin is taken out from square plate, removes the preservative film of covering, and a small amount of surface water is sprayed daily.
Seedling replanting
Transplanted by when seedling sporinite grows 3 and above true leaf, matrix selects insemination and emergence effect most during transplanting
Good peat：River sand=1:1 matrix, transplanting container uses 64-72 holes disk or bore 5cm common plasticses flowerpots, takes out seedling
When should not destroy root system, and with certain sowing media.Pour permeable after transplanting, be put into maintenance in warmhouse booth, counted after 30d
Survival rate is up to 95.6%.
Claims (4)
1. a kind of method of Adiantum reniforme L var sinense Y. X. Lin spore seed propagation, it is characterised in that comprise the following steps
The selection of experiment material
The spore of Adiantum reniforme L var sinense Y. X. Lin is just opened in mid or late June to maturation between early August, period when spore bag hole, and spore is also not
Band leaf harvesting in time is used as sowing material when being scattered；
Sowing media, container selection and treatment
By sowing media（1）Leaf mould or（2）Peat：Perlite is 3.5 in mass ratio：1 mixture or（3）River sand or
（4）Peat：River sand is 1 in mass ratio:1 mixture；High-temperature sterilization 30min, rear overlay film in high-temperature sterilization pot or dryer
The 3-4h that is exposed to the sun under the sun is standby；Sowing container is bore 13-17cm common plasticses flowerpot or square plate, is soaked with boiling water plus washing powder
Bubble 20min after clean, dry it is stand-by；
Adiantum reniforme L var sinense Y. X. Lin spore is sowed
Treated sowing media is loaded into flowerpot, is filled to away from basin mouthful 3-5cm, basin heelpiece crock or foam are easy to draining, are broadcast
Plant after matrix device is finished and pour permeable with the carbendazim solution for being configured to 800 times, by surfacing, remove surface impurity；Prepare
Blank sheet of paper or filter paper, sporangiorus is gently stirred with blade, allows spore to become scattered about on paper, after by scraps of paper doubling, spore is uniformly spilt
Fall within flowerpot surface, it is impossible to rewater；Preservative film is covered on flowerpot, the water spray moisturizing of preservative film inner side prepares several plastic squares
Disk, 3-5cm clear water is filled after cleaning, and the flowerpot that will broadcast spore is put into wherein；The square plate that flowerpot will be filled is positioned over warmhouse booth
Sowing time management is carried out in interior or outdoor bower or culturing room；
Adiantum reniforme L var sinense Y. X. Lin spore sowing time management
Sowing is first 7 days, and clear water in square plate is exchanged daily, and adds 2000 times of carbendazim, clear to the sprinkling of preservative film inner side
Water moisturizing；In the 7-30 days, clear water in square plate was exchanged in every 3 days, to the sprinkling clear water moisturizing of preservative film inner side, and in time clearly
Wash the liver moss and other floating materials of growth in square plate；Then clear water in square plate was exchanged in 5-7 days after 30 days, to preservative film inner side
Sprinkling clear water moisturizing, the liver moss and other floating materials for being grown in cleaning square plate in time；After sporinite seedling is formed, by flowerpot from
Taken out in square plate, remove the preservative film of covering, a small amount of surface water is sprayed daily；
Seedling replanting
Transplanted into sowing media by when seedling sporinite grows 3 and above true leaf, transplanting container uses 64-72 holes
Disk or bore 5cm common plasticses flowerpots, root system should not be destroyed when taking out seedling, and with certain sowing media；Poured after transplanting
It is permeable, maintenance in warmhouse booth is put into, can be completed after 30d and survival rate can be counted.
2. the method for the Adiantum reniforme L var sinense Y. X. Lin spore seed propagation described in claim 1, it is characterised in that described sowing media is
Peat：River sand is 1 in mass ratio:1 mixture.
3. the method for the Adiantum reniforme L var sinense Y. X. Lin spore seed propagation that claim 1 is told, it is characterised in that Adiantum reniforme L var sinense Y. X. Lin spore is broadcast
Plant in step, sowing media device pours permeable after finishing with the carbendazim solution for being configured to 800 times, can not be watered after having sowed.
4. the method for the Adiantum reniforme L var sinense Y. X. Lin spore seed propagation described in claim 1, it is characterised in that Adiantum reniforme L var sinense Y. X. Lin spore is broadcast
In kind of step, will fill flowerpot square plate be positioned over outdoor tree shade under carry out sowing time management.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901842A (en) * | 2018-06-28 | 2018-11-30 | 中国长江三峡集团有限公司 | A method of induction Adiantum reniforme L var sinense Y. X. Lin sporinite |
| CN109006439A (en) * | 2018-09-10 | 2018-12-18 | 北京昌回林海生态科技有限公司 | A kind of venushair fern sapling multiplication method based on hole tray live streaming spore method |
| CN110226385A (en) * | 2019-06-11 | 2019-09-13 | 深圳市铁汉生态环境股份有限公司 | A kind of type of seeding of pteridophyte |
| CN110383992A (en) * | 2019-08-28 | 2019-10-29 | 中国长江三峡集团有限公司 | A kind of method of Adiantum reniforme L var sinense Y. X. Lin division propagation |
| CN111096173A (en) * | 2020-01-06 | 2020-05-05 | 浙江省亚热带作物研究所 | Method for promoting pen container tree development through adjusting environmental factors |
| CN113133385A (en) * | 2020-01-20 | 2021-07-20 | 上海辰山植物园 | Spore propagation method of rare or endangered lotus leaf Adiantum |
| CN115500265A (en) * | 2022-10-28 | 2022-12-23 | 中国长江三峡集团有限公司 | Method for quickly sowing and breeding adiantum lotus leaf spores to obtain seedlings |
| GB2622316A (en) * | 2022-09-09 | 2024-03-13 | China Three Gorges Corp | Method for simulating high-efficiently breeding unique rare Adiantum nelumboides in wild environment |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901842A (en) * | 2018-06-28 | 2018-11-30 | 中国长江三峡集团有限公司 | A method of induction Adiantum reniforme L var sinense Y. X. Lin sporinite |
| CN109006439A (en) * | 2018-09-10 | 2018-12-18 | 北京昌回林海生态科技有限公司 | A kind of venushair fern sapling multiplication method based on hole tray live streaming spore method |
| CN110226385A (en) * | 2019-06-11 | 2019-09-13 | 深圳市铁汉生态环境股份有限公司 | A kind of type of seeding of pteridophyte |
| CN110383992A (en) * | 2019-08-28 | 2019-10-29 | 中国长江三峡集团有限公司 | A kind of method of Adiantum reniforme L var sinense Y. X. Lin division propagation |
| CN110383992B (en) * | 2019-08-28 | 2021-06-25 | 中国长江三峡集团有限公司 | Method for plant division propagation of nelumbo nucifera |
| CN111096173A (en) * | 2020-01-06 | 2020-05-05 | 浙江省亚热带作物研究所 | Method for promoting pen container tree development through adjusting environmental factors |
| CN113133385A (en) * | 2020-01-20 | 2021-07-20 | 上海辰山植物园 | Spore propagation method of rare or endangered lotus leaf Adiantum |
| GB2622316A (en) * | 2022-09-09 | 2024-03-13 | China Three Gorges Corp | Method for simulating high-efficiently breeding unique rare Adiantum nelumboides in wild environment |
| CN115500265A (en) * | 2022-10-28 | 2022-12-23 | 中国长江三峡集团有限公司 | Method for quickly sowing and breeding adiantum lotus leaf spores to obtain seedlings |
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