CN102144490A - Propagation method of stenoloma chusana spores - Google Patents
Propagation method of stenoloma chusana spores Download PDFInfo
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- CN102144490A CN102144490A CN2011100232113A CN201110023211A CN102144490A CN 102144490 A CN102144490 A CN 102144490A CN 2011100232113 A CN2011100232113 A CN 2011100232113A CN 201110023211 A CN201110023211 A CN 201110023211A CN 102144490 A CN102144490 A CN 102144490A
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Abstract
The invention relates to a propagation method of stenoloma chusana spores, which comprises the following steps of: mature stenoloma chusana spore collecting, ground substance disinfecting, spore sterilizing, spore germinating, zygote forming, gametophyte growing, antiphyte growing, seedling transplanting and the like. The method can be used for fast propagating the stenoloma chusana, is simple in the preparation of the cultivation ground substance, is simple and convenient in operation method, is high in operability, and has the advantages of being small in investment and high in yield, so that the transplanting survival rate of the spore seedlings reaches more than 85%.
Description
Technical field
The present invention relates to the sporogenesis method of Stenoloma chusana, relate to a kind of propagation method that can breed Stenoloma chusana (Sphenomerischinenses) spore fast specifically.
Background technology
Stenoloma chusana is that the Lindsaeaceae Stenoloma chusana belongs to (Sphenomeris) plant, is a kind of middle-size and small-size Lu Sheng fern, is widely distributed in the torrid zone and subtropical zone, is born under the low mountain forest and the roadside, and happiness light is drought-enduring.Stenoloma chusana leaf color jade green or yellowish green, the blade division is thinner, and blade profile is longer, and form is elegant, and the attitude grace has higher ornamental value, and can be drought-resistant.Light is than the benefit isolated planting in the greenery patches, sheet is planted, group planting all can, also can make scape with other plant arrangement, also can be used for potted plant viewing and admiring, be a kind of better fern of viewing and admiring.The Stenoloma chusana herb is medicinal, and the effect of heat-clearing, dampness removing, detoxifcation is arranged, and it is in all ferns, contains the highest fern of flavone component, and important medical value is arranged.The prospect of marketing is preferably arranged.
The breeding of Stenoloma chusana can be adopted the mode of plant division and sporogenesis, and the modes of reproduction speed of plant division is slower, and reproduction coefficient is low, is difficult to be applied to commodity production, and at present about the research of the conventional sporogenesis of Stenoloma chusana also seldom.
Summary of the invention
The objective of the invention is to develop a kind of simple, practical and method of breeding Stenoloma chusana efficiently.
The present invention adopts NaClO that spore is sterilized, and with potassium permanganate matrix is carried out disinfection, and adopts the method for conventional spore sowing and gametophyte growth, have simple to operate, less investment obtains the characteristics of a large amount of juvenile sporophytes in a short time, thereby realizes purpose of the present invention.
The propagation method of Stenoloma chusana of the present invention may further comprise the steps:
(1) spore on collection robust growth Stenoloma chusana blade top is wrapped with filter paper, drops in the sterile water, fully soaks, and drops into 3～10% the interior 5min of immersion of NaClO solution again, afterwards fast with sterile distilled water flushing 3 times.
(2) spore that will sterilize and clean is made into suspension, evenly be sprayed at the pH value and be on 6.0～-7.0 the soil matrix (sand+perlite+peat (volume ratio 1: 1: 1)), 23～33 ℃ of temperature, intensity of illumination is at 3000～5000lx, light application time is at 9～12h/d, under the condition of humidity 65～80%, in the greenhouse, cultivate.Seedling with 3～4 spires appears and grows up to until young spore seedling.
(3) with 3～4 spires of tool, the young spore transplantation of seedlings of height more than 2cm is in the seed tray of the matrix of (sand, perlite and peat (volume ratio 1: 1: 2)) mixing, in 23～33 ℃ of intensities of illumination of temperature at 3000～5000lx, light application time is at 9～12h/d, under the condition of humidity 65～80%, in the greenhouse, cultivate.
The present invention utilizes NaClO solution that spore is carried out disinfection, with potassium permanganate matrix is carried out disinfection, with the two combination, realize the spore aseptic culture, be applied to the sporogenesis of Stenoloma chusana, prevented growing of germ contamination in the early stage seeding and seedling raising process and other irrelevant organism effectively, it is big to have reproduction coefficient, can carry a large amount of young spore seedling of arch in a short time, practicality simple to operate, characteristics with low cost.The transplanting survival rate of children's spore seedling helps the commercialization production of Stenoloma chusana more than 85%.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1:
In the spore maturation about 30% of Stenoloma chusana, cut the blade that has ripe spore, be placed on the clean blank sheet of paper and dry in the shade.After treating that spore is scattered fully, different according to spore with sporangial quality, select the higher spore 0.03g of purity by hand, wrap with filter paper, drop into and soak 5min in the 5%NaClO solution, use aseptic water washing afterwards fast.
Husky, perlite and 1: 1: 1 by volume ratio of peat mixed to obtain pH value be 6.0～7.0 sowing media, the Stenoloma chusana spore of sterilizing is sowed on the matrix for preparing.23～33 ℃ of cultivation temperature, intensity of illumination are at 3000～5000lx, and light application time is mixed and crossed 65-80% at 9～12h/d.Sow after 8 days, observe under magnifying glass, spore is sprouted successively, the visible spore germination of naked eyes behind the 15d, gametophyte develops into plates behind the 25d, and the gametophyte of Stenoloma chusana grows the ripe heart-shaped stage behind the 45d, behind the 56d, begin to have young spore seedling to occur, juvenile sporophyte appears in beginning in a large number behind the 80d.
Husky, perlite and by volume 1: 1: 2 ratio of peat mixed obtain the soil matrix that the pH value is 6.0～7.0 seedling-cultivating tray.Treat to transplant to seedling-cultivating tray when juvenile sporophyte grows to the 2cm height, in the greenhouse, cultivate 25～33 ℃ of cultivation temperature, intensity of illumination 3000-5000lx, light application time 9-12h/d, humidity 65～80%.Look weather condition, spray water every day 2～3 times, the juvenile sporophyte transplanting survival rate reaches more than 85%.
Embodiment 2:
In the spore maturation about 50% of Stenoloma chusana, cut the blade that has ripe spore, be placed on the clean blank sheet of paper and dry in the shade.After treating that spore is scattered fully,, select the spore 0.03g of the higher water of purity by hand, wrap, drop into and soak 5min in the 5%NaClO solution, wash with sterile distilled water fast afterwards with filter paper according to sporangial quality difference.
Husky, perlite and 1: 1: 1 by volume ratio of peat mixed to obtain pH value be 6.0～7.0 sowing media, the Stenoloma chusana spore of sterilizing is sowed on the matrix for preparing.23～33 ℃ of cultivation temperature, intensity of illumination 3000～5000lx, light application time is at 9-12h/d, humidity 65-80%.Sow after 8 days, observe under magnifying glass, spore is sprouted successively, the visible spore germination of naked eyes behind the 15d, gametophyte is grown plates behind the 25d, and the gametophyte of Stenoloma chusana grows the ripe heart-shaped stage behind the 45d, behind the 56d, begin to have young spore seedling to occur, juvenile sporophyte appears in beginning in a large number behind the 80d.
Husky, perlite and 1: 1: 2 by volume ratio of peat mixed obtain the soil matrix that the pH value is 6.0～7.0 seedling-cultivating tray.Treat to transplant to the seedling dish when juvenile sporophyte grows to the 2cm height, in the greenhouse, cultivate 25～33 ℃ of cultivation temperature, intensity of illumination 3000～5000lx, light application time 9～12h/d, humidity 65～80%.Look weather condition, spray water every day 2～3 times, the transplanting survival rate of juvenile sporophyte reaches more than 85%.
Embodiment 3:
In the spore maturation about 30% of Stenoloma chusana, cut the blade that has ripe spore, be placed on the clean blank sheet of paper and dry in the shade.After treating that spore is scattered fully, different according to spore with sporangial quality, select the higher spore 0.03g of purity by hand, wrap with filter paper, drop into and soak 5min in the 10%NaClO solution, wash with sterile distilled water fast afterwards.
Husky, perlite and 1: 1: 1 by volume ratio of peat mixed to obtain pH value be 6.0～7.0 sowing media, the Stenoloma chusana spore of sterilizing is sowed on the matrix for preparing.23～33 ℃ of cultivation temperature, intensity of illumination be at 3000～5000lx, and light application time is at 9～12h/d, humidity 65-80%.Sow after 8 days, under magnifying glass, observe, spore is sprouted successively, the visible spore germination of 20d naked eyes, and gametophyte develops into plates behind the 45d, the gametophyte of Stenoloma chusana grows the ripe heart-shaped stage behind the 70d, behind the 90d, begin to have young spore seedling to occur, begin to occur more juvenile sporophyte behind the 120d, it is slow slightly to grow, the juvenile sporophyte poor growth.
Husky, perlite and 1: 1: 2 by volume ratio of peat mixed obtain the soil matrix that the pH value is 6.0～7.0 seedling-cultivating tray.Treat to transplant to seedling-cultivating tray when juvenile sporophyte grows to the 2cm height, in the greenhouse, cultivate 25～33 ℃ of cultivation temperature, intensity of illumination 3000～5000lx, light application time 9～12h/d, humidity 65～80%.Look weather condition, spray water every day 2～3 times, the transplanting survival rate of juvenile sporophyte reaches more than 70%.
Embodiment 4: check experiment
In the spore maturation about 30% of Stenoloma chusana, cut the blade that has ripe spore, be placed on the clean blank sheet of paper and dry in the shade.After treating that spore is scattered fully, different according to spore with sporangial quality, select the higher spore 0.03g of purity by hand, wrap with filter paper, soak 5min with sterile distilled water.
Husky, perlite and 1: 1: 1 by volume ratio of peat mixed to obtain pH value be 6.0～7.0 sowing media, the Stenoloma chusana spore of sterilizing is sowed on the matrix for preparing.23～33 ℃ of cultivation temperature, intensity of illumination be at 3000～5000lx, and light application time is at 9～12h/d, humidity 65-80%.Sow after 9 days, observe under magnifying glass, spore is sprouted successively, the visible spore germination of naked eyes behind the 15d, simultaneously, mould, green alga and liver moss appear in stromal surface in earthen basin, and gametophyte develops into plates behind the 25d, and the gametophyte of Stenoloma chusana grows the ripe heart-shaped stage behind the 45d, behind the 55d, begin to have a small amount of young spore seedling to occur, because assorted bacterium, algae and bryophyte appearance are more, the quantity of young spore seedling is less slightly.
Husky, perlite and 1: 1: 2 by volume ratio of peat mixed obtain the soil matrix that the pH value is 6.0～7.0 seedling-cultivating tray.Treat to transplant to seedling-cultivating tray when juvenile sporophyte grows to the 2cm height, in the greenhouse, cultivate 25～33 ℃ of cultivation temperature, intensity of illumination 3000～5000lx, light application time 9～12h/d, humidity 65～80%.Look weather condition, spray water every day 2～3 times, the transplanting survival rate of juvenile sporophyte reaches more than 85%.
Claims (4)
1. a Stenoloma chusana sporogenesis method is characterized in that: adopt NaClO that spore is carried out disinfection, use KMnO
4Sowing media and transplanting medium are carried out disinfection, and the various living contaminantses of having avoided spore to occur in germination process can effectively carry out the breeding of Stenoloma chusana spore, have simple to operately, and less investment obtains the characteristics of a large amount of juvenile sporophytes in a short time.
2. employing NaClO according to claim 1 carries out disinfection to spore, it is characterized in that: the optimum N aClO concentration of spore sterilization is 3-5%.
3. the KMnO that uses according to claim 1
4Sowing media and transplanting medium are carried out disinfection, it is characterized in that: the pH value of sowing media is 6.0～7.0, substrate composition (sand+perlite+peat (volume ratio 1: 1: 1)), the pH value of transplanting medium is 6.0～7.0, substrate composition (sand, perlite and peat (volume ratio 1: 1: 2)).
4. employing NaClO according to claim 1 carries out disinfection to spore, uses KMnO
4Sowing media and transplanting medium are carried out disinfection, it is characterized in that: the environmental condition of the common sterilization of spore and matrix, 23～33 ℃ of temperature, intensity of illumination is at 3000～5000lx, light application time is at 9～12h/d, sows under the condition of humidity 65～80% and transplants.
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| CN2011100232113A CN102144490A (en) | 2011-01-21 | 2011-01-21 | Propagation method of stenoloma chusana spores |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613075A (en) * | 2012-03-21 | 2012-08-01 | 江汉大学 | Method for breeding floating ferns of endangered ferns |
| CN103348846A (en) * | 2013-06-10 | 2013-10-16 | 哈尔滨师范大学 | Reproduction method of isoetes yunguiensis |
| CN104604493A (en) * | 2015-01-26 | 2015-05-13 | 河南科技学院 | Cyrtomium fortunei spore reproduction method |
| CN106688842A (en) * | 2016-11-17 | 2017-05-24 | 中国长江三峡集团公司 | Seeding and propagating method of lotus leaf adiantum spore |
| CN111096173A (en) * | 2020-01-06 | 2020-05-05 | 浙江省亚热带作物研究所 | Method for promoting pen container tree development through adjusting environmental factors |
-
2011
- 2011-01-21 CN CN2011100232113A patent/CN102144490A/en active Pending
Non-Patent Citations (5)
| Title |
|---|
| 《中国优秀硕士学位论文全文数据库（农业科技辑）》》 20070415 黄超群 南京市几种蕨类植物孢子繁殖及抗旱性研究 1-5 , 第4期 * |
| 《中国种业》 20050215 赵秀芳 蕨类植物的孢子繁殖技术 , 第2期 * |
| 《云南植物研究》 20081215 任冰如等 乌蕨孢子萌发研究 第30卷, 第6期 * |
| 《安徽农业科学》 20100901 孙起梦等 几种蕨类植物的孢子繁殖试验研究 第38卷, 第25期 * |
| 《资源开发与市场》 20101215 张国珍等 乌蕨孢子繁殖技术的研究 第26卷, 第12期 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613075A (en) * | 2012-03-21 | 2012-08-01 | 江汉大学 | Method for breeding floating ferns of endangered ferns |
| CN103348846A (en) * | 2013-06-10 | 2013-10-16 | 哈尔滨师范大学 | Reproduction method of isoetes yunguiensis |
| CN103348846B (en) * | 2013-06-10 | 2015-06-17 | 哈尔滨师范大学 | Reproduction method of isoetes yunguiensis |
| CN104604493A (en) * | 2015-01-26 | 2015-05-13 | 河南科技学院 | Cyrtomium fortunei spore reproduction method |
| CN106688842A (en) * | 2016-11-17 | 2017-05-24 | 中国长江三峡集团公司 | Seeding and propagating method of lotus leaf adiantum spore |
| CN111096173A (en) * | 2020-01-06 | 2020-05-05 | 浙江省亚热带作物研究所 | Method for promoting pen container tree development through adjusting environmental factors |
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Application publication date: 20110810 |