CN1786149A - Field separation and activity preservation method of agaric spore - Google Patents
Field separation and activity preservation method of agaric spore Download PDFInfo
- Publication number
- CN1786149A CN1786149A CN 200410082897 CN200410082897A CN1786149A CN 1786149 A CN1786149 A CN 1786149A CN 200410082897 CN200410082897 CN 200410082897 CN 200410082897 A CN200410082897 A CN 200410082897A CN 1786149 A CN1786149 A CN 1786149A
- Authority
- CN
- China
- Prior art keywords
- spore
- paper
- aseptic
- agaric
- vessel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to field separation of parasol mushroom spore. Its operation steps are collecting parasol mushroom fruiting body; cutting its unwanted stipe by aseptic cutter; the treated fruiting body is set on the aseptic paper with its hut turning up; sticking absorbent paper to the inner wall; vessel is covered on the aseptic paper to make the fruiting body is in airproof room; setting for four to eight hours; the spore will be ejected to the aseptic paper to form spore print; preserving the paper by sealing. The advantages of the invention are that spore separation effect is good; applicable range is wild; and spore keep alive time is long.
Description
Technical field
Separate in the field that the present invention relates to the agaric spore, and separate and keepalive method in specifically a kind of field of agaric spore.
Background technology
Open-air separation of traditional agaric spore and keepalive method are roughly: the agaric sporophore that collects is cut away unnecessary stem with aseptic cutter, with the sporophore cap after handling up lamella be put on the filter paper down, cover sporophore with vessel then, placed 4 to 8 hours, spore will launch on the filter paper, form spore print, will collect the filter paper of spore print then and keep well; Open-air separation of conventional agaric spore and keepalive method shortcoming are: if it is not enough to deposit the interior humidity of the vessel of sporophore, just can not obtain spore print and maybe can only obtain a small amount of spore, and this method can only be separated the spore than the sporophore of comparatively fresh; The spore that is separated to does not seal preservation, and its keep-alive time is no more than 1 time-of-week (but asynchronism(-nization) of different types of spore keep-alive) usually.
Summary of the invention
The object of the present invention is to provide the field of the agaric spore that a kind of spore separation is effective, the keep-alive time is long to separate and keepalive method.
For achieving the above object, the technical solution used in the present invention is:
Separate and keepalive method in a kind of field of agaric spore, the agaric sporophore that collects is cut away unnecessary stem with aseptic cutter, with the sporophore cap after handling up lamella place on the aseptic paper down, there is the thieving paper of clear water to be affixed on the inwall of vessel with absorption, cover aseptic paper with vessel then, sporophore is in the enclosed space of vessel and aseptic paper formation, placed 4 to 8 hours, spore will launch on the aseptic paper, forms spore print; The aseptic paper of collecting spore print folded put into bag sealing and preserve.
Described aseptic paper can be filter paper or the blank sheet of paper of the bacterium of going out; The water regain of thieving paper is generally 10～20ml and gets final product, and described sealing bag is generally plastics bag.
The present invention has following advantage:
1. spore separation is effective, widely applicable.Drawing 10～20ml clear water with thieving paper during spore separation is affixed on the inwall of vessel, thereby make and keep 100% humidity in the vessel, the spore that this method not only can be separated the sporophore more complete than comparatively fresh can also separate the spore of some imperfect or older sporophores.
2. the spore keep-alive time is long.Spore that the present invention is separated to is preserved with the plastics bag of sealing, at normal temperature (15～25 ℃) but keep-alive 1～5 time-of-week under the condition, low temperature (4 ℃) but keep-alive 5～7 time-of-weeks (but asynchronism(-nization) of different types of spore keep-alive) under the condition.
Embodiment
Embodiment 1
Agaric (as honey mushroom etc.) sporophore that collects is cut away unnecessary stem with aseptic cutter; with the sporophore cap after handling up lamella be put in down on the aseptic paper (as the filter paper of the bacterium of going out or blank sheet of paper etc.); drawing 10～20ml clear water with thieving paper is affixed on the inwall of vessel; thereby make and keep 100% humidity in the vessel; cover sporophore with vessel then; placed 4 to 8 hours; spore will launch on the aseptic paper; form spore print; the aseptic paper that to collect spore print then folds the plastics bag of putting into sealing; but keep-alive separates the agaric spore that obtains, and the keep-alive time can reach for 2 weeks to 6 weeks.
Claims (4)
1. separate and keepalive method in the field of an agaric spore, it is characterized in that: the agaric sporophore that collects is cut away unnecessary stem with aseptic cutter, with the sporophore cap after handling up lamella place on the aseptic paper down, there is the thieving paper of clear water to be affixed on the inwall of vessel with absorption, cover aseptic paper with vessel then, sporophore is in the enclosed space of vessel and aseptic paper formation, placed 4 to 8 hours, spore will launch on the aseptic paper, forms spore print; The aseptic paper of collecting spore print folded put into bag sealing and preserve.
2. separate and keepalive method according to the field of the described agaric spore of claim 1, it is characterized in that: described aseptic paper is went out filter paper or the blank sheet of paper of bacterium.
3. separate and keepalive method according to the field of the described agaric spore of claim 1, it is characterized in that: the water regain of described thieving paper is 10～20ml.
4. separate and keepalive method according to the field of the described agaric spore of claim 1, it is characterized in that: described bag is plastics bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100828973A CN1314796C (en) | 2004-12-08 | 2004-12-08 | Field separation and activity preservation method of agaric spore |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100828973A CN1314796C (en) | 2004-12-08 | 2004-12-08 | Field separation and activity preservation method of agaric spore |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1786149A true CN1786149A (en) | 2006-06-14 |
| CN1314796C CN1314796C (en) | 2007-05-09 |
Family
ID=36783762
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100828973A Expired - Fee Related CN1314796C (en) | 2004-12-08 | 2004-12-08 | Field separation and activity preservation method of agaric spore |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1314796C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101220344B (en) * | 2008-01-15 | 2011-05-04 | 江西农业大学 | Filter paper sheet diaphragmatic foramen suspension spore ejection method |
| CN103109647A (en) * | 2013-03-08 | 2013-05-22 | 北京农学院 | Method for collecting bolete spores |
| CN103688746A (en) * | 2013-12-03 | 2014-04-02 | 重庆市汀来绿色食品开发有限公司 | Method for collecting and culturing edible fungus spores |
| CN106612982A (en) * | 2016-12-21 | 2017-05-10 | 广东省微生物研究所 | Efficient pleurotus tuber-regium multi-spore isolation method and multi-spore isolation device used in same |
| CN106688842A (en) * | 2016-11-17 | 2017-05-24 | 中国长江三峡集团公司 | Seeding and propagating method of lotus leaf adiantum spore |
| CN107937244A (en) * | 2017-11-20 | 2018-04-20 | 信阳农林学院 | A kind of method that spore print makes box and large-sized fungal sporule print is made of the making box field |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1049793C (en) * | 1995-12-29 | 2000-03-01 | 伍宗文 | Agaricus tabularis "Fenba" fungus seed and culture method and cultivation technology |
-
2004
- 2004-12-08 CN CNB2004100828973A patent/CN1314796C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101220344B (en) * | 2008-01-15 | 2011-05-04 | 江西农业大学 | Filter paper sheet diaphragmatic foramen suspension spore ejection method |
| CN103109647A (en) * | 2013-03-08 | 2013-05-22 | 北京农学院 | Method for collecting bolete spores |
| CN103688746A (en) * | 2013-12-03 | 2014-04-02 | 重庆市汀来绿色食品开发有限公司 | Method for collecting and culturing edible fungus spores |
| CN103688746B (en) * | 2013-12-03 | 2015-09-02 | 重庆市汀来绿色食品开发有限公司 | A kind of collection cultural method of edible mushrooms spore |
| CN106688842A (en) * | 2016-11-17 | 2017-05-24 | 中国长江三峡集团公司 | Seeding and propagating method of lotus leaf adiantum spore |
| CN106612982A (en) * | 2016-12-21 | 2017-05-10 | 广东省微生物研究所 | Efficient pleurotus tuber-regium multi-spore isolation method and multi-spore isolation device used in same |
| CN107937244A (en) * | 2017-11-20 | 2018-04-20 | 信阳农林学院 | A kind of method that spore print makes box and large-sized fungal sporule print is made of the making box field |
| CN107937244B (en) * | 2017-11-20 | 2023-06-16 | 信阳农林学院 | Spore print manufacturing box and method for manufacturing large fungus spore print in field by adopting manufacturing box |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1314796C (en) | 2007-05-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1786149A (en) | Field separation and activity preservation method of agaric spore | |
| CN102871218B (en) | Method of oxygen reduction, dehumidification and mold prevention during tobacco storage | |
| CN1188056C (en) | Freshness preserving gadget | |
| CN102198877A (en) | Anti-pollution aging method for putting black tea into packaging container with ventilated extraction valve having sealing and filter film | |
| CN105010500B (en) | A kind of physical fresh-keeping method of stem tuber agricultural products | |
| CN101411355B (en) | Method for processing dried lichee and dried longan | |
| CN107624496A (en) | A kind of cultural method of organic big flower mushroom | |
| KR102054083B1 (en) | Manufacturing apparatus and manufacturing methods for liquid fertilizer | |
| CN109549248B (en) | Alcoholization mature mildew tobacco leaf mold removal treatment method | |
| KR20150004007A (en) | method of extraction for essence of Korean Dendropanax | |
| CN104823629A (en) | Method for promoting healing of cuttings of southern pines in high temperature seasons | |
| CN212354849U (en) | Tea storage device | |
| CN211672283U (en) | Fresh-keeping device after picking of shiny-leaved yellowhorn tender shoots | |
| CN109549247B (en) | Method for removing mildew from tobacco leaves mildewed in alcoholization period | |
| CN107177514A (en) | A kind of purity is the preparation method of 100% morchella mother culture | |
| CN201678147U (en) | Bottle | |
| CN205180260U (en) | Tealeaves fermentor that withers | |
| CN214095084U (en) | Special refrigerator of glossy ganoderma | |
| CN218755505U (en) | Secondary fermentation device for bio-organic fertilizer | |
| CN214830373U (en) | Inoculation system for solid-state food brewing | |
| CN104521619B (en) | Heliogreenhouse drainage system | |
| CN106612982A (en) | Efficient pleurotus tuber-regium multi-spore isolation method and multi-spore isolation device used in same | |
| CN210275457U (en) | Take gold thread lotus group of micropore fungus isolation ventilated membrane to cultivate group bag | |
| CN107567959A (en) | A kind of production method of Ganoderma lucidum | |
| CN204707534U (en) | A kind of novel mushroom cultivation bag |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |