CN107937244A - A kind of method that spore print makes box and large-sized fungal sporule print is made of the making box field - Google Patents
A kind of method that spore print makes box and large-sized fungal sporule print is made of the making box field Download PDFInfo
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- CN107937244A CN107937244A CN201711157842.8A CN201711157842A CN107937244A CN 107937244 A CN107937244 A CN 107937244A CN 201711157842 A CN201711157842 A CN 201711157842A CN 107937244 A CN107937244 A CN 107937244A
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- spore
- box
- supporting plate
- fixed pin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N3/00—Spore forming or isolating processes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
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- Life Sciences & Earth Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of spore print to make box, including the box body with upper cover, horizontally disposed supporting plate center position, which upwardly extends, in box body is provided with fixed pin, is threaded through at the spore printing paper center being laid on supporting plate in fixed pin, supporting plate is provided with box body inner bottom plating and highly adjusts component；Water-absorbing sponge layer is equipped with upper cover.The invention also discloses the method for being made large-sized fungal sporule print in the wild of the making box.It is simple in structure that spore print of the present invention makes box, easy to operate, field acquisition to fresh macro fungi fructification can carry out spore print making at the first time, fructification is fresh, and spore ejection rate is high, and Production Time is short and production effect is good；Fixed pin and spongy layer are provided with due to making in box, sterile water can be added to avoid cap displacement in the process of walking, in spongy layer and be required with ensureing in box that humidity meets that spore print makes；Box compact is made, it is convenient to carry at any time；Can batch making spore print, be adapted to student to practise in the wild use.
Description
Technical field
The present invention relates to the making of spore print, and box is made more particularly, to a kind of spore print, should the invention further relates to using
Make the method that box field makes large-sized fungal sporule print.
Background technology
Spore print is the result of study of Canadian scholar Bu Le nineteen twenty-twos, i.e., when the fructification of basidiomycetes is ripe, cap
Open, small water droplet can be secreted out of between basidiospore and stigma, since internal turgescence acts on, water droplet is departed from stigma rapidly with spore
Outwards ejection, spore, which is fully fallen in, just forms distinctive pattern on paper, which is spore print.Because various basidiomycetes spores
Form, the color of son are different, and size, the form of lamella are also different, so spore print is identification, identifies that fungus specimen is indispensable
One of basis.
The macro fungi of spore print can be made, it is in classification to belong to Eumycota Basidiomycetes more.Make the key of spore print
It is to grasp the time, it is necessary to select cap to open, but carried out during maturation within reason.The fresh mark that must be gathered with the same day
This, and should try one's best and avoid being dehydrated, otherwise fructification water content reduces, and turgescence, which reduces, will be unfavorable for the ejection of spore, while fungi
The lamella and cap structure of fructification must stand intact, and otherwise can influence the form of spore print because fructification deforms, cause
It is fubaritic.
The production method of traditional spore print mainly has three kinds：1. cutting away unnecessary stem, do a stent with thin wire and consolidate
Surely cap is lived, after standing on paper, is got well with outer cover cover and stands some hours, spore will launch and spore print is formed on paper；2.
Unnecessary stem is cut away, directly cap is buckled on paper, some hours are stood after outer cover cover is good, spore will launch shape on paper
Into spore print；3. a duck eye is dug in paper center, lamella is downward, in stem insertion hole, then paper is moved together with thalline and is put
On the cup for filling half water, some hours are stood, spore will launch and spore print is formed on paper.Above-mentioned production method is all
It is to carry out indoors, also selects the environment of quiet dead-beat.But fructification it is general water content it is all larger and very soft,
Gathering and be easy to be dehydrated in transportational process, damage, spore print can not be produced, therefore, the mirror of some rare macro fungis
Fixed just extremely difficult, there is presently no the method for producing spore print in the wild.Chinese invention patent（CN 1786149A）Disclose
A kind of field separation of agaric spore and keepalive method（The method for making spore print）, specific method is：Field acquisition is arrived
Fructification takes back interior, cuts away unnecessary stem, and lamella is placed on aseptic paper downward, and the blotting paper for having clear water with suction is affixed on vessel
Inner wall on, then cover aseptic paper with vessel, place 4-8 it is small when.But this method is still to carry out indoors, and it is not to use
Fresh, the intact fructification of collection makes spore print at the first time, is affixed on blotting paper on inner wall of utensil also very
Easily come off, damage spore print, therefore, use this method manufacture effect of spore print and bad.
The content of the invention
It is an object of the invention to provide a kind of spore print from place to use limitation to make box, and the present invention also provides adopt
The method that large-sized fungal sporule print is made of the making box field.
To achieve the above object, the present invention can take following technical proposals：
Spore print of the present invention makes box, including the box body with upper cover, horizontally disposed supporting plate center in the box body
Upwardly extended at position and be provided with fixed pin, the fixed pin is threaded through at the spore printing paper center being laid on the supporting plate
On, on the box body inner bottom plating being provided with supporting plate highly adjusts component；Water-absorbing sponge layer is equipped with the upper cover.
The supporting plate highly adjusts component by the internally threaded sleeve being vertically fixed on the box body inner bottom plating, is screwed in institute
State the adjustment screw upper end in internally threaded sleeve and the bottom surface of the supporting plate is fixedly connected with.
The fixed pin is the hexagonal pyramid structure of point upward.
The water-absorbing sponge layer is to be close to the fixation spongy layer of the upper interior surface.
Multilayer adjustment spongy layer is movably set with the fixed spongy layer.
The spore printing paper is equally divided into black and white two parts along its diameter, and surface is uniformly coated with egg white on it
Liquid or glue.
The method for making large-sized fungal sporule print in the wild using present invention making box, comprises the following steps：
The first step, spore printing paper is threaded through in fixed pin, and the form that field acquisition is arrived is intact and parachute-opening but not overdone
Macro fungi fructification will be cut away with blade at the neat cap of stem, and remaining stem is inserted in fixed pin, makes lamella close to spore
Printing paper；
Second step, according to the size thickness of cap, the height that component adjusts fixed pin is highly adjusted by supporting plate；Adjustment is adjusted again
The number of plies of spongy layer, after making making box closed, spore printing paper, cap, the contact of spongy layer composition but the sandwich structure not extruded；
3rd step, sprays into sterile water in spongy layer, moistens sponge；
4th step, upper cover and box body are screwed；It is labelled on box is made, indicate numbering, locality, fungi name and beginning
Make spore print time, be then horizontally placed in containing box and carry with, avoid excessively rocking and overturning, on car to be returned to or
After institute, box will be made in time and will be taken out, the horizontal positioned and spore print that is completed after standing 2-12 hour.
To improve the production effect of spore print and extending the holding time, it is added dropwise on the spore print surface that the 4th step completes
Fresh albumen, makes the egg white nature that blows slowly pour out and be covered in spore print surface；After to be dried, spore print is fixed up, you can carries out
Take pictures, preserve and identify use.
The present invention spore print make box it is simple in structure, it is easy to operate, it can be achieved that spore print field make, and use should
The method that making box makes spore print in the wild is simple, and the time is short, and effect is good, efficient, workable.Specifically this hair
Bright beneficial effect may be embodied in following aspects：
1st, the field for realizing spore print makes, and the fresh macro fungi fructification collected in the wild is at the first time can be into
Row spore print makes, and fructification is fresh, and spore ejection rate is high, and Production Time is short and production effect is good；
2nd, fixed pin and spongy layer are provided with due to making in box, can be to avoid cap displacement in the process of walking, in spongy layer
Sterile water can be added to ensure that humidity meets that spore print makes requirement in box；Box compact is made, it is convenient to carry at any time；Also may be used
Batch making spore print, is especially suitable for student and practises in the wild use.
Brief description of the drawings
Fig. 1, Fig. 2 are the upper cover and box body structure schematic diagram of the present invention respectively.
Fig. 3, Fig. 4 are the sectional view of Fig. 1, Fig. 2 respectively.
Fig. 5 is the top view of Fig. 2.
Embodiment
As shown in Fig. 1 ~ 5, spore print of the invention makes box, including the box body 2 with upper cover 1, and upper cover 1 and box body 2 are logical
Cross the screw thread being mutually matched（Or buckle）It is flexibly connected, water-absorbing sponge layer is equipped with upper cover 1, which includes tight
Stick the fixation spongy layer 1.1 of 1 inner surface of lid（Thickness is 0.5mm or so）, if necessary, can be with fixed spongy layer 1.1
Place one layer or 2 ~ 3 layers adjustment spongy layer 1.2（The thickness of every layer of adjustment spongy layer is also 0.5mm or so）；Level is set in box body 2
2.1 center position of supporting plate put upwardly extends the hexagonal pyramid structure fixed pin 2.2 for being provided with point upward, is laid in supporting plate
It is threaded through at 2.3 center of spore printing paper on 2.1 in fixed pin 2.2, spore printing paper 2.3 can use quality thicker and not hygroscopic
Circular paper make, it is diametrically equally divided into black and white two parts, fixed pin 2.2 is at the center in black and white line of demarcation
Pass through（In the wild during collect specimen, since the color of spore can not be predicted in advance, so spore printing paper is used black and white
Two parts form, and the mushroom spore color such as run into is deep, clearly can show spore print on the paper of white portion, such as run into bacterium
Class spore is of light color, clearly can show spore print on the paper of black portions）；Uniformly smeared in the upper surface of spore printing paper 2.3
There are egg white solution or glue（Glue can use animal glue, 5% gum arabic or common glue）, facilitate spore absorption in spore print
Above paper.Used for convenience of field, improve producing efficiency, spore printing paper 2.3 can be prefabricated in advance, after smearing egg white solution or glue certainly
So air-dry spare；Supporting plate, which is provided with, on 2 inner bottom plating of box body highly adjusts component.
During actual fabrication, supporting plate highly adjusts component can be by the internally threaded sleeve 2.4 that is vertically fixed on 2 inner bottom plating of box body
Formed with the adjustment screw 2.5 being screwed in internally threaded sleeve, the upper end of adjustment screw 2.5 and the bottom surface of supporting plate 2.1 are consolidated
It is connected as a single entity.Certainly, supporting plate, which highly adjusts component, can also use other common structures.
The method that special making box using the present invention makes large-sized fungal sporule print in the wild comprises the following steps：
Spore printing paper 2.3, is threaded through in fixed pin 2.2 by the first step first, and the form that field acquisition is arrived is intact and parachute-opening
But not overdone macro fungi fructification will be cut away with blade at the neat cap of stem, and remaining stem is inserted in fixed pin 2.2, is made
Lamella, since fixed pin 2.2 is hexagonal pyramid structure, ensures that fungus sporophore will not rotate in fixed pin close to spore printing paper；
Second step, according to the size thickness of cap, the height that component adjusts fixed pin 2.2 is highly adjusted by supporting plate；Adjust again
The number of plies of spongy layer 1.2 is adjusted, after making making box closed, spore printing paper 2.3, cap, spongy layer composition are contacted but do not extruded
Sandwich structure；
3rd step, sprays into sterile water in spongy layer, and water spray can not be excessive, moistens sponge；
4th step, upper cover 1 and box body 1 are screwed；It is labelled on box is made, indicate numbering, locality, fungi name and open
Beginning makes the time of spore print, is then horizontally placed in containing box and carries with, avoids excessively rocking and overturning, on car to be returned to
Or after institute, box will be made in time and taken out, it is horizontal positioned and after standing 2-12 hour（Different macro fungis forms spore print
Time it is different）The spore print to be completed；It is careful to open upper cover 1, cap is gently removed with tweezers, removes spore printing paper
2.3, that is, a spore print identical with lamella or tube arrangement mode is obtained, is then taken pictures in time to fresh spore print
And record.To improve the production effect of spore print and extending the holding time, fresh egg white can be carefully dropped on spore print,
Allow its nature that blows slowly to pour out and be covered in spore print surface, wait it is dry after, spore print is fixed up.At this time, spore print has made
Into can be taken pictures, be preserved, identifying use.
Claims (9)
1. a kind of spore print makes box, including with upper cover（1）Box body（2）, it is characterised in that：The upper cover（1）Interior laying
There is water-absorbing sponge layer；The box body（2）Interior horizontally disposed supporting plate（2.1）Center position, which upwardly extends, is provided with fixed pin
（2.2）, it is laid in the supporting plate（2.1）On spore printing paper（2.3）The fixed pin is threaded through at center（2.2）On, institute
State box body（2）Supporting plate is provided with inner bottom plating and highly adjusts component.
2. spore print according to claim 1 makes box, it is characterised in that：The supporting plate highly adjusts component by longitudinally solid
It is scheduled on the box body（2）Internally threaded sleeve on inner bottom plating（2.4）, the adjustment screw that is screwed in the internally threaded sleeve
（2.5）Upper end and the supporting plate（2.1）Bottom surface be fixedly connected with.
3. spore print according to claim 1 makes box, it is characterised in that：The fixed pin（2.2）For point upward
Hexagonal pyramid structure.
4. spore print according to claim 1 makes box, it is characterised in that：The water-absorbing sponge layer is to be close to the upper cover
（1）The fixation spongy layer of inner surface（1.1）.
5. spore print according to claim 4 makes box, it is characterised in that：In the fixed spongy layer（1.1）Upper activity
It is provided with multilayer adjustment spongy layer（1.2）.
6. spore print according to claim 1 makes box, it is characterised in that：The spore printing paper（2.3）Put down along its diameter
Black and white two parts are divided into, surface is uniformly coated with egg white solution or glue on it.
7. spore print according to claim 1 makes box, it is characterised in that：The upper cover（1）And box body（2）By mutual
Matched screw thread or buckle are flexibly connected.
8. the method for making large-sized fungal sporule print using box field is made described in claim 1, it is characterised in that：
The first step, by spore printing paper（2.3）It is threaded through fixed pin（2.2）On, the form that field acquisition is arrived is intact and parachute-opening
But not overdone macro fungi fructification will be cut away with blade at the neat cap of stem, and remaining stem is inserted in fixed pin（2.2）On,
Make lamella close to spore printing paper（2.3）；
Second step, according to the size thickness of cap, component is highly adjusted by supporting plate and adjusts fixed pin（2.2）Height；Adjust again
Section adjustment spongy layer（1.2）The number of plies, after making making box closed, spore printing paper（2.3）, cap, spongy layer composition contact but not
The sandwich structure of extruding；
3rd step, sprays into sterile water in spongy layer, moistens sponge；
4th step, by upper cover（1）And box body（1）Screw；Make box on it is labelled, indicate numbering, locality, fungi name
With the time for starting from spore print, then it is horizontally placed in containing box and carries with, avoids excessively rocking and overturning, wait to return to
On car or after institute, box will be made in time and will be taken out, the horizontal positioned and spore print that is completed after standing 2-12 hour.
9. the method according to claim 8 for making large-sized fungal sporule print, it is characterised in that：Complete in the 4th step
Spore print surface fresh albumen is added dropwise, the egg white nature that blows slowly is poured out and is covered in spore print surface；After to be dried, spore print is solid
Decide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711157842.8A CN107937244B (en) | 2017-11-20 | 2017-11-20 | Spore print manufacturing box and method for manufacturing large fungus spore print in field by adopting manufacturing box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711157842.8A CN107937244B (en) | 2017-11-20 | 2017-11-20 | Spore print manufacturing box and method for manufacturing large fungus spore print in field by adopting manufacturing box |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107937244A true CN107937244A (en) | 2018-04-20 |
| CN107937244B CN107937244B (en) | 2023-06-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711157842.8A Active CN107937244B (en) | 2017-11-20 | 2017-11-20 | Spore print manufacturing box and method for manufacturing large fungus spore print in field by adopting manufacturing box |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1786149A (en) * | 2004-12-08 | 2006-06-14 | 中国科学院沈阳应用生态研究所 | Field separation and activity preservation method of agaric spore |
| JP2007053928A (en) * | 2005-08-23 | 2007-03-08 | Kinokkusu:Kk | New fungal strain and method for culturing new fruit body |
| CN202396183U (en) * | 2012-01-17 | 2012-08-29 | 常熟理工学院 | Edible fungus spore collector |
| CN203884238U (en) * | 2014-06-09 | 2014-10-22 | 鸡西市食用菌科学研究所 | Spore collector |
| CN204298386U (en) * | 2014-12-09 | 2015-04-29 | 贵州大学 | A kind of plant disease moisturizing device |
| CN204550630U (en) * | 2015-03-30 | 2015-08-12 | 贵州大学 | A kind of modified version culture dish |
| CN209383760U (en) * | 2017-11-20 | 2019-09-13 | 信阳农林学院 | Large-sized fungal sporule prints field and makes box |
-
2017
- 2017-11-20 CN CN201711157842.8A patent/CN107937244B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1786149A (en) * | 2004-12-08 | 2006-06-14 | 中国科学院沈阳应用生态研究所 | Field separation and activity preservation method of agaric spore |
| JP2007053928A (en) * | 2005-08-23 | 2007-03-08 | Kinokkusu:Kk | New fungal strain and method for culturing new fruit body |
| CN202396183U (en) * | 2012-01-17 | 2012-08-29 | 常熟理工学院 | Edible fungus spore collector |
| CN203884238U (en) * | 2014-06-09 | 2014-10-22 | 鸡西市食用菌科学研究所 | Spore collector |
| CN204298386U (en) * | 2014-12-09 | 2015-04-29 | 贵州大学 | A kind of plant disease moisturizing device |
| CN204550630U (en) * | 2015-03-30 | 2015-08-12 | 贵州大学 | A kind of modified version culture dish |
| CN209383760U (en) * | 2017-11-20 | 2019-09-13 | 信阳农林学院 | Large-sized fungal sporule prints field and makes box |
Non-Patent Citations (2)
| Title |
|---|
| MARCUS ROPER等: "Explosively launched spores of ascomycete fungi havedrag-minimizing shapes" * |
| 邢路军: "大型菌类标本的采集制作及技巧" * |
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| Publication number | Publication date |
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| CN107937244B (en) | 2023-06-16 |
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