CN206562441U - A kind of occupy-place formula cell scratch test device - Google Patents
A kind of occupy-place formula cell scratch test device Download PDFInfo
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- CN206562441U CN206562441U CN201720246251.7U CN201720246251U CN206562441U CN 206562441 U CN206562441 U CN 206562441U CN 201720246251 U CN201720246251 U CN 201720246251U CN 206562441 U CN206562441 U CN 206562441U
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- placed layer
- box body
- occupy
- division board
- test device
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Abstract
The utility model provides a kind of occupy-place formula cell scratch test device, including box body, division board and lid, placed layer one, placed layer two and placed layer three are set gradually in box body from top to bottom, one group of division board is equipped with placed layer one, placed layer two and placed layer three, in division board, be uniformly laterally arranged multigroup groove on bottom plate, isolation baffle plate is connected with bottom plate by neck, the outer wall upper end extension of box body sets antiskid thread, the internal thread being adapted with antiskid thread is set in lid, and box body and lid are connected through a screw thread;The apparatus structure is simple, it is easy to use, utilize the mode of isolation baffle plate occupy-place, form straight, the uniform cut of width, and cut hecatomeral cells is not by the mechanical damage produced in traditional experiment, the division board of different size is stored by box body, experiment health is ensure that applied widely while, there is wide applicability in medical experiment field.
Description
Technical field
The utility model belongs to the technical field of medical experiment device, more specifically, and the utility model is related to a kind of cell
The technical field of scratch test device.
Background technology
Cell scratch test, also referred to as wound healing assay, are a useful experiments of in vitro study cell migration.It is former
Reason is that the acellular area of a blank is artificially manufactured in the cell monolayer of bed board, then to this acellular area
The cell at edge is observed;The cell at these edges can proceed by migratory movement, and Landfill covering is whole acellular
Region, is contacted with each other together again.
When carrying out cell scratch test, after cell is adherent in culture dish, traditional method is the rifle using liquid-transfering gun
Head is streaked in the centre of attached cell, artificially causes one of cut, afterwards the width of Timing measurement cut, and then obtain wound to be cured
The cell migration data of conjunction.Traditional method has the drawback that:1st, manual cut, it is impossible to ensure the consistent of each cut;2、
A piece of cell sometimes can be scratched while cell is drawn, mechanical damage is caused to the cell at the edge of cut;If the 3, cultivated
If there be coating protein ware bottom, pipette tips are streaked, it is likely that hurt coating, and then had influence on cell migration, as a result just very
It is which factor causes conclusive influence that hardly possible, which judges,;4th, the width of cut is difficult measurement.
The content of the invention
For the deficiency of state of the art, in order to solve that current cell scratch experiment cut is inconsistent, it is difficult to measure, holds
Mechanical damage, the technical problem impacted to experiment are easily caused to cell, the utility model provides a kind of occupy-place formula cell
Scratch test device, compared with prior art, the apparatus structure are simple, easy to use, utilize the mode of isolation baffle plate occupy-place, shape
Straighe, the uniform cut of width, and cut hecatomeral cells passes through box body not by the mechanical damage produced in traditional experiment
The division board of different size is stored, stackable storage, experiment health is ensure that applied widely while, in medical treatment
Experiment field has wide applicability.
In order to solve the above problems, the technical solution adopted in the utility model is as follows:
A kind of occupy-place formula cell scratch test device that the utility model is provided, including box body, division board and lid, box body
In set gradually from top to bottom on placed layer one, placed layer two and placed layer three, placed layer one, placed layer two and placed layer three
Provided with one group of division board, in division board, be uniformly laterally arranged multigroup groove on bottom plate, and isolation baffle plate is connected with bottom plate by neck
Connect, the outer wall upper end extension of box body sets the internal thread for setting and being adapted with antiskid thread in antiskid thread, lid, box body and box
Lid is connected through a screw thread.
In the utility model, placed layer one and placed layer two are in annular shape, and the internal diameter of placed layer one is more than placed layer two
Internal diameter, the external diameter of placed layer one is identical with the external diameter of placed layer two, and the region in the internal diameter of placed layer two is set to placed layer three.
In the utility model, bottom plate is in the form of annular discs, and the quantity of groove is more than the quantity of isolation baffle plate.
In the utility model, groove and isolation baffle plate are in inverted T-shape shape, and groove and isolation baffle plate, which are adapted, to be set.
In the utility model, air-vent is uniformly arranged on bottom plate, air-vent is in strip.
In the utility model, handle is set, handle uses built-in handle on lid.
The technical scheme provided using the utility model can obtain following beneficial effect:
A kind of occupy-place formula cell scratch test apparatus structure that the utility model is provided is simple, easy to use, utilizes isolation
The mode of baffle plate occupy-place, form straight, the uniform cut of width, and cut hecatomeral cells in traditional experiment by not producing
Mechanical damage, is stored the division board of different size by box body, stackable storage, is ensured applied widely while
Experiment health, in medical experiment field has wide applicability.
Brief description of the drawings
Fig. 1 is shown as structural representation of the present utility model.
Fig. 2 is shown as the box body structure schematic diagram of the utility model offer.
Fig. 3 is shown as the box body top view of the utility model offer.
Fig. 4 is shown as the partition plate structure schematic diagram of the utility model offer.
In figures 1-4:1-box body, 11-outer wall, 111-antiskid thread, 12-placed layer one, 13-placed layer two,
14-placed layer three, 2-division board, 21-bottom plate, 211-groove, 212-air-vent, 22-isolation baffle plate, 3-lid,
31-handle.
Embodiment
1-4 and embodiment, are described in further detail to embodiment of the present utility model below in conjunction with the accompanying drawings, but
The utility model device is not limited to following embodiments.
In the utility model, for the ease of describing, each part is relative in a kind of occupy-place formula cell scratch test device
The description of position relationship is with reference to the accompanying drawings 1 Butut mode to be described, such as:The position relationships such as upper and lower, left and right be according to
Determined according to the Butut direction of Figure of description 1.
A kind of occupy-place formula cell scratch test device that the utility model is provided, including box body(1), division board(2)And box
Lid(3), box body(1)In set gradually placed layer one from top to bottom(12), placed layer two(13)With placed layer three(14), placed layer
One(12), placed layer two(13)With placed layer three(14)On be equipped with one group of division board(2), division board(2)In, bottom plate(21)On
Be uniformly laterally arranged multigroup groove(211), isolation baffle plate(22)With bottom plate(21)Connected by neck, box body(1)Outer wall
(11)Upper end extension sets antiskid thread(111), lid(3)Interior setting and antiskid thread(111)Adaptable internal thread, box body
(1)And lid(3)It is connected through a screw thread.
In the utility model, placed layer one(12)With placed layer two(13)In annular shape, placed layer one(12)Internal diameter it is big
In placed layer two(13)Internal diameter, placed layer one(12)External diameter and placed layer two(13)External diameter it is identical, placed layer two(13)
Internal diameter in region be set to placed layer three(14).
In the utility model, bottom plate(21)It is in the form of annular discs, groove(211)Quantity be more than isolation baffle plate(22)Quantity,
Groove in different spacing can be needed according to experiment(211)Upper setting isolation baffle plate(22), bottom plate(21)And isolation baffle plate(22)
It is adapted when back-off is placed with culture dish shape and height is less than culture dish.
In the utility model, groove(211)And isolation baffle plate(22)It is in inverted T-shape shape, groove(211)And isolation baffle plate
(22)It is adapted and sets.
In the utility model, bottom plate(21)On be uniformly arranged air-vent(212), facilitate the respiration of cell, air-vent
(212)In strip, Convenient tweezerses steadily grip division board(2).
In the utility model, lid(3)Upper setting handle(31), handle(31)Using built-in handle, facilitate multiple
Device can be arranged under the overlay storage.
In a kind of occupy-place formula cell scratch test device provided using the utility model, handle is held(31)Turn on box
Lid(3), groove is clipped in the tweezers point of tweezers(211)It is interior by division board(2)Take out successively, by division board(2)Tip upside down on culture
In ware, cell growth is treated to a certain extent, remove division board(2), so that form that one straight, after the uniform cut of width,
Carry out the measurement work of cell scratch experiment, division board(2)Cleaning puts back to box body after air-drying(1)It is interior, cover lid(3)Screw;
Bottom plate(21)And isolation baffle plate(22)Dismounting and change can be needed according to experiment.
A kind of occupy-place formula cell scratch test apparatus structure that the utility model is provided is simple, easy to use, utilizes isolation
Baffle plate(22)The mode of occupy-place, forms straight, the uniform cut of width, and cut hecatomeral cells is not produced by traditional experiment
Raw mechanical damage, passes through box body(1)By the division board of different size(2)Store, stackable storage, applied widely
While ensure that experiment health, there is wide applicability in medical experiment field.
As described above, you can preferably realize the utility model, the above embodiments are only to of the present utility model excellent
Select embodiment to be described, not scope of the present utility model is defined, do not depart from the utility model design spirit
On the premise of, various modifications and improvement that those of ordinary skill in the art make to the technical solution of the utility model all should fall
In the protection domain for entering the utility model determination.
Claims (6)
1. a kind of occupy-place formula cell scratch test device, including box body, division board and lid, it is characterised in that from upper in box body
Down set gradually and be equipped with one on placed layer one, placed layer two and placed layer three, placed layer one, placed layer two and placed layer three
In group division board, division board, be uniformly laterally arranged multigroup groove on bottom plate, and isolation baffle plate is connected with bottom plate by neck, box body
Outer wall upper end extension the internal thread for setting and being adapted with antiskid thread in antiskid thread, lid is set, box body and lid pass through
Threaded connection.
2. a kind of occupy-place formula cell scratch test device as claimed in claim 1, it is characterised in that the described He of placed layer one
Placed layer two is in annular shape, and the internal diameter of placed layer one is more than the internal diameter of placed layer two, external diameter and the placed layer two of placed layer one
External diameter is identical, and the region in the internal diameter of placed layer two is set to placed layer three.
3. a kind of occupy-place formula cell scratch test device as claimed in claim 1, it is characterised in that described bottom plate is in disk
Shape, the quantity of groove is more than the quantity of isolation baffle plate.
4. a kind of occupy-place formula cell scratch test device as claimed in claim 1, it is characterised in that described groove and isolation
Baffle plate is in inverted T-shape shape, and groove and isolation baffle plate, which are adapted, to be set.
5. a kind of occupy-place formula cell scratch test device as claimed in claim 1, it is characterised in that uniform on described bottom plate
Air-vent is set, and air-vent is in strip.
6. a kind of occupy-place formula cell scratch test device as claimed in claim 1, it is characterised in that set on described lid
Handle, handle uses built-in handle.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201720246251.7U CN206562441U (en) | 2017-03-14 | 2017-03-14 | A kind of occupy-place formula cell scratch test device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201720246251.7U CN206562441U (en) | 2017-03-14 | 2017-03-14 | A kind of occupy-place formula cell scratch test device |
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Publication Number | Publication Date |
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CN206562441U true CN206562441U (en) | 2017-10-17 |
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CN201720246251.7U Expired - Fee Related CN206562441U (en) | 2017-03-14 | 2017-03-14 | A kind of occupy-place formula cell scratch test device |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109055219A (en) * | 2018-09-17 | 2018-12-21 | 重庆医科大学附属儿童医院 | A kind of cell scar Healing Experiments device |
CN109234143A (en) * | 2018-10-31 | 2019-01-18 | 扬州大学 | A kind of cell scoring devices and its application method |
-
2017
- 2017-03-14 CN CN201720246251.7U patent/CN206562441U/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109055219A (en) * | 2018-09-17 | 2018-12-21 | 重庆医科大学附属儿童医院 | A kind of cell scar Healing Experiments device |
CN109234143A (en) * | 2018-10-31 | 2019-01-18 | 扬州大学 | A kind of cell scoring devices and its application method |
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Legal Events
Date | Code | Title | Description |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171017 Termination date: 20180314 |