CN206986175U

CN206986175U - A kind of culture dish for cell scratch experiment

Info

Publication number: CN206986175U
Application number: CN201720528013.5U
Authority: CN
Inventors: 黎明; 徐艳; 贺美; 邹纯
Original assignee: Central South University
Current assignee: Central South University
Priority date: 2017-05-12
Filing date: 2017-05-12
Publication date: 2018-02-09
Anticipated expiration: 2027-05-12

Abstract

The utility model discloses a kind of culture dish for cell scratch experiment, the inner chamber of the culture dish is placed with cut frame, the cut frame includes cut shaped bar and vertical rod, some cut shaped bars contact with the bottom surface fitting of culture dish, and culture dish bottom surface is divided into some regions, the vertical rod is connected with all cut shaped bars, forms integral cut frame.The utility model can use in existing Nostoc commune Vanch ware, carry out cell culture after cut form-holder is placed in culture dish again, afterwards directly take out cut form-holder from culture dish, directly form the cut of unified specification.The utility model is simple, convenient, and cost is cheap, directly can be used in common Tissue Culture Dish, and applicability is high, and the cut homogeneity of formation is strong, and comparative experimental data is more accurate.

Description

A kind of culture dish for cell scratch experiment

Technical field

The utility model belongs to Bioexperiment technology, and in particular to a kind of culture dish for cell scratch experiment.

Background technology

Cell scarification is one of method for detecting tumor cell migration ability, and principle is：Cell is adherent in culture dish Afterwards, streaked using the pipette tips of liquid-transfering gun in the centre of attached cell, artificially cause one of wound (cut), Timing measurement is drawn afterwards The width of trace, and then the cell migration data of wound healing are obtained, the growth transfer ability of cell is judged with this.The general meeting of experiment Control group and experimental group are set, and experimental group is typically the increase in the processing of the factors such as medicine, allogenic gene, by detecting different groups Between cell migration Distance Judgment cell transfer ability.Although cell scarification is commonly used to detect the transfer ability of cell Research, but it still has manual cut and cannot be guaranteed the consistent of each cut.

The patent of Application No. 201710000010.9, the patent of Application No. 201620744530.1 are all a kind of to utilize cunning Dynamic cut carries out the device of scratch test for cell, manufactures unified cut, but this mode every time by cut pin Still have the disadvantage that；1) refinement is needed, easily a piece of cell can be scratched while cell is drawn, to the edge of cut Cell cause mechanical damage；2) if also having coating protein if culture dish bottom, cut pin streaks, it is likely that hurts bag Quilt, and then cell migration is had influence on, as a result just it is difficult that judgement is which factor causes conclusive influence；3) Timing measurement is drawn The width of trace, the change of scratch width over time is calculated, when choosing cut measurement point, inevitably introduces subjectivity Error (artificially have chosen migration results and meet point expected from experiment)；4) this kind of mode is required to carry out phase to the cover plate of culture dish The improvement answered, the production cost of culture dish is improved, and cut pin does not have unified standard, and the cover plate applicability of culture dish is not It is high.In summary, traditional cell scratch experiment repeatability is bad, the elaboration convincingness deficiency for experimental result, improved The defects of complicated, operation becomes more meticulous be present in cell scoring devices.

Utility model content

The utility model solve technical problem be：For drawbacks described above existing for existing cell scratch experiment, there is provided A kind of new culture dish for cell scratch experiment.

The utility model adopts the following technical scheme that realization：

A kind of culture dish for cell scratch experiment, the inner chamber of the culture dish are placed with cut frame, the cut frame Including cut shaped bar and vertical rod, the bottom surface fitting of some cut shaped bars and culture dish contacts, and by culture dish bottom surface It is divided into some regions, the vertical rod is connected with all cut shaped bars, forms integral cut frame.

Further, bottom surface radial distribution of the cut shaped bar along culture dish, and have in the bottom center of culture dish There is a common connection intersection point, the vertical rod is connected in the connection point of intersection with all cut shaped bars.

Further, the section of the cut shaped bar is semicircle, and it flats down to be bonded with the bottom surface of culture dish and connect Touch, its circular arc is face-up.

Further, the one end of the cut shaped bar away from connection intersection point contacts with the side wall of culture dish.

Further, the top of the vertical rod is provided with handle, and the cut frame is positioned in culture dish, the top of the handle End height is no more than the cover plate of culture dish.

In the utility model, the cut frame uses transparent glass material.

The utility model can use in existing Nostoc commune Vanch ware, enter again after cut form-holder is placed in culture dish Row cell culture, directly cut form-holder is taken out from culture dish afterwards, directly form the cut of unified specification.

From the above mentioned, the beneficial effects of the utility model are：(1) it is simple and convenient, workable；(2) it is easy to make Simply, cost is cheap, directly can be used in common Tissue Culture Dish, and applicability is high；(3) the cut homogeneity formed is strong, And more existing scoring devices are compared, the present embodiment places cut frame before cell culture, and taking out cut frame will not be to cell Itself is damaged, and excludes that cut is improper that deviation is influenceed to caused by experimental data, makes comparative experimental data more accurate.

The utility model is described further below in conjunction with the drawings and specific embodiments.

Brief description of the drawings

Fig. 1 is the six orifice plate culture dish schematic diagrames for not placing cut frame in embodiment.

Fig. 2 is the schematic diagram for placing cut frame in embodiment in the culture dish of sky.

Fig. 3 is the cut frame schematic diagram in embodiment.

Fig. 4 is the schematic diagram of the culture dish culture cell of the placement cut frame in embodiment.

Fig. 5 is the cut schematic diagram that the culture dish in embodiment is formed after cut frame is taken out.

Label in figure：The orifice plates of 1- six, 2- cover plates, 3- culture dish chambers, 4- cut framves, 41- cut shaped bars, 42- vertical rods, 43- handles, 5- cell culture mediums, 6- cuts.

Embodiment

Referring to Fig. 1, the present embodiment illustrates by taking one or six orifice plates 1 as an example, and six orifice plates 1 are with six culture dish inner chambers 3 Contrast cell culture utensil, including six orifice plates 1 and cover plate 2 with six culture dish inner chambers, mistake of the cover plate 2 in cell culture The bacterium completely cut off in journey in extraneous air influences to caused by the inner cell culture of culture dish inner chamber 3.

As shown in Figures 2 and 3, before cell is cultivated, the cut frame 4 in Fig. 3 is placed into one of culture dish inner chamber 3 Interior, the cut frame 4 in the present embodiment includes three cut shaped bars, 41, vertical rods 42 and a handle 43, wherein three The effect of cut shaped bar 41 is that the bottom surface of culture dish inner chamber 3 is separated into several regions, and vertical rod 42 is by cut shaped bar 41 An entirety is connected into, handle 43 is then used to pick and place cut frame 4.

Specifically, cut shaped bar 41 in the present embodiment is the body of rod of semi-circular section, and along the bottom of culture dish inner chamber 3 Face radial distribution, flatting down for the semicircle body of rod contact with the fitting of the bottom surface of culture dish inner chamber 3, arc surface then upward, cut into The height of shape bar 41 is unsuitable too high, and in the incubation of culture cell, cell can be moved by the bottom surface transition of culture dish inner chamber 3 Breeding is moved to arc surface, cell is well mixed in Initial stage of culture in culture dish bottom surface, such as the cut in the present embodiment The height of shaped bar 41 is 1mm.Bottom center of the three cut shaped bars 41 of the present embodiment in culture dish inner chamber 3 connects phase Hand over, in the point of intersection and three integrally connecteds of cut shaped bar 41, vertical rod can use the cylinder body of rod for vertical rod 42, and its top sets ball The handle 43 of shape, it is easy to integrally pick and place cut frame.The one end of cut shaped bar 41 away from center junction then will be with training Support the side wall contact of ware inner chamber 3, ensure the cut to be formed the bottom surface of culture dish inner chamber 3 can be separated into three it is completely isolated Region

In actual applications, cut frame 4 is shaped using transparent glass sintering, and its whole height will ensure be put into culture Behind ware inner chamber, the top of its handle 43 is no more than the height of cover plate 2 of six orifice plates 1, cover plate 2 is reliably covered in six orifice plates On, avoid the occurrence of and form gap between the orifice plate 1 of cover plate 2 and six.The cut that the quantity of cut shaped bar 41 can be carried out as needed Experiment determines, preferably includes but does not limit three, four.

As shown in figure 4, after cut frame 4 is placed in culture dish inner chamber 3, cell culture medium is carried out in culture dish inner chamber 5 and the placement of cell, then cover cover plate 2 and allow cell to carry out free breeding.After the time of culture is reached, cover plate 2 is opened, Cut frame 4 is taken out from culture dish inner chamber 3 by tweezers, the cut shaped bar 41 of cut frame 4 is in the bottom surface of culture dish inner chamber 3 The cut 6 of three same width is formd, as shown in Figure 5, using the center intersection point of three cuts as with reference to selection cut measurement Point, the width of Timing measurement cut, changed with time by calculating scratch width, obtain the data of cell migration.

Embodiment above describes the advantages of general principle of the present utility model and principal character and the utility model, one's own profession The technical staff of industry is it should be appreciated that the utility model is not restricted to the described embodiments, described in above-described embodiment and specification Simply illustrate concrete operating principle of the present utility model, on the premise of the spirit and scope of the utility model is not departed from, this reality Various changes and modifications are also had with new, these changes and improvements are both fallen within claimed the scope of the utility model, this Scope is claimed by appended claims and its equivalent thereof in utility model.

Claims

A kind of 1. culture dish for cell scratch experiment, it is characterised in that：The inner chamber of the culture dish is placed with cut frame, institute Stating cut frame includes cut shaped bar and vertical rod, and some cut shaped bars contact with the bottom surface fitting of culture dish, and will training Support ware bottom surface and be divided into some regions, the vertical rod is connected with all cut shaped bars, forms integral cut frame.
2. a kind of culture dish for cell scratch experiment according to claim 1, the cut shaped bar is along culture dish Bottom surface radial distribution, and there is a common connection intersection point in the bottom center of culture dish, the vertical rod is handed in the connection It is connected at point with all cut shaped bars.
3. a kind of culture dish for cell scratch experiment according to claim 2, the section of the cut shaped bar are Semicircle, it flats down contacts with the fitting of the bottom surface of culture dish, and its circular arc is face-up.
4. a kind of culture dish for cell scratch experiment according to claim 3, the cut shaped bar is away from connection One end of intersection point contacts with the side wall of culture dish.
5. a kind of culture dish for cell scratch experiment according to claim 4, the top of the vertical rod is provided with handle, The cut frame is positioned in culture dish, and the tip height of the handle is no more than the cover plate of culture dish.
6. a kind of culture dish for cell scratch experiment according to any one of claim 1-5, the cut frame is adopted With transparent glass material.