CN210974678U

CN210974678U - Novel cell culture bottle improve device

Info

Publication number: CN210974678U
Application number: CN201921836555.4U
Authority: CN
Inventors: 赵宁; 舒晓刚; 吴亮; 周自立; 张旭旦; 韩胜博; 鲍海俊
Original assignee: Union Hospital Tongji Medical College Huazhong University of Science and Technology
Current assignee: Union Hospital Tongji Medical College Huazhong University of Science and Technology
Priority date: 2019-10-29
Filing date: 2019-10-29
Publication date: 2020-07-10
Anticipated expiration: 2029-10-29

Abstract

The utility model provides a novel cell culture bottle improve device relates to the cell culture bottle field. This novel cell culture bottle improves device, including well cultivation room, the top of well cultivation room is provided with cultivates the room, the fixed bottle that is provided with in top of going up cultivation room, the top of going up the bottle is provided with the bottle lid, the bottom of well cultivation room is provided with down cultivates the room. This novel cell culture bottle improve device through the interior clear wall convenience that sets up in last bottle when cultivateing the cell, can reduce the clearance dead angle in the culture chamber, increase and can wash for the culture chamber after accomplishing once cultivateing in the culture chamber, reduce the influence that the dead angle in the culture chamber caused remaining of cell and cause for the experiment, it piles up to reduce the cell of the indoor dead angle department of culture, it has the dead angle of clearance to reduce the application of sample rifle, conveniently increase the application of sample rifle and carry out abluent cleanliness factor for culture chamber inside, conveniently increase the accuracy of experiment next time.

Description

Novel cell culture bottle improve device

Technical Field

The utility model relates to a cell culture bottle technical field specifically is a novel cell culture bottle improve device.

Background

Cell culture refers to a method for making cells survive, grow, reproduce and maintain main structures and functions by simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro, and is an essential process for the whole biological engineering technology and one of the biological cloning technologies. In the process of cell culture, as the culture time is prolonged and the cells are divided continuously, the cells contact with each other to generate contact inhibition, the growth speed is reduced or even stopped, and on the other hand, the growth is not facilitated or poisoning is generated due to insufficient nutrients and metabolite accumulation. Therefore, diluting the cultured cells and then re-inoculating the diluted cells into a cell culture flask for re-culture is an essential and important link for the cell culture process.

However, the shape design of the cell culture bottle on the market at present has an obvious defect, that is, the dead angle of the culture bottle is an area where the sample adding gun cannot blow directly during the cell passage, which means that the cells at the left and right upper corners of the culture bottle cannot be diluted during the passage, so that the cells in the area are contacted with each other for a long time, the nutrition is insufficient, the metabolite is accumulated, the survival state of the cells is remarkably reduced, and the reliability of the experimental result is seriously influenced.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

Not enough to prior art, the utility model provides a novel cell culture bottle improve device has solved the dead angle of original blake bottle for the cell is at passage in-process, and the application of sample rifle can realize the abluent problem to arbitrary region in the blake bottle.

(II) technical scheme

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: the utility model provides a novel cell culture bottle improves device, includes well cultivation room, the top of well cultivation room is provided with cultivates the room, the fixed bottle that is provided with in top of going up cultivation room, the top of going up the bottle is provided with the bottle lid, the bottom of well cultivation room is provided with down cultivates the room.

Preferably, both ends of the middle culture chamber are provided with sealing threads, and both ends of the middle culture chamber are respectively connected with the lower culture chamber and the upper culture chamber through the sealing threads.

Preferably, the bottom of the lower culture chamber is fixedly provided with an abrasion-increasing convex body.

Preferably, the bottom of the upper culture chamber is provided with a thread sealing opening, and the inside of the upper culture chamber is provided with a culture inner cavity.

Preferably, an inner washing wall is arranged inside the upper bottle body.

(III) advantageous effects

The utility model provides a novel cell culture bottle improve device. Has the following beneficial effects:

1. this novel cell culture bottle improve device through last internal clear wall convenience of setting up of bottle when cultivateing the cell, can reduce the clearance dead angle in the culture chamber, conveniently accomplish in the culture chamber and can wash comprehensively for the culture chamber after once cultivating, reduce the influence that the dead angle in the culture chamber caused remaining of cell and cause for the experiment, it piles up to reduce the cell of the indoor dead angle department of culture, it has the dead angle of clearance to reduce the application of sample rifle, conveniently increase the application of sample rifle and carry out abluent cleanliness factor for culture chamber inside, conveniently increase the accuracy of experiment next time, make things convenient for the application of sample rifle to give the angle of culture chamber clearance.

2. This novel cell culture bottle improve device increases the frictional force that the convex body was convenient for increase the bottom for the blake bottle when using through the increase mill that cultivates the room bottom down and set up, increase the blake bottle bottom with the contact dynamics of desktop, the probability of toppling over takes place for convenient the reduction when the blake bottle is placed, the influence that the inside cell culture caused is toppled over in convenient reduction blake bottle when carrying out the cultivation takes place, the damage of cell when convenient the reduction cultivates in the blake bottle, it receives the collision and causes the probability of damage to reduce the blake bottle, the stability of increase blake bottle.

3. This novel cell culture bottle improves device and conveniently connects well culture room and last culture room through the thread sealing mouth that last culture room bottom set up and culture inner chamber, the mode through threaded connection can increase the cell of cultivateing in the blake bottle, conveniently cultivate different cell and the same cell, increase the quantity of cultivateing the room in the blake bottle, conveniently carry out the multicellular culture in a blake bottle, conveniently contrast when cultivateing, reduce the use of blake bottle, the length of thread sealing mouth is greater than sealed screw, conveniently seal the screw, reduce the influence that outside gas caused to the cultivation of inside.

Drawings

FIG. 1 is a front view of the present invention;

FIG. 2 is a schematic view of the present invention;

FIG. 3 is a plan view of the lower chamber of the present invention;

FIG. 4 is a sectional view of the upper chamber of the present invention;

fig. 5 is a cross-sectional view of the upper bottle body of the present invention.

In the figure: 1 middle culture room, 2 upper culture room, 3 upper bottle body, 4 bottle cap, 5 sealing screw thread, 6 lower culture room, 7 polishing convex body, 8 screw thread sealing port, 9 culture inner cavity and 10 inner washing wall.

Detailed Description

The invention is explained in more detail below with reference to the figures and examples.

The embodiment of the utility model provides a novel cell culture bottle improve device, as shown in fig. 1-5, including well culture room 1, the top of well culture room 1 is provided with culture room 2, goes up the fixed upper flask body 3 that is provided with in top of culture room 2, and the top of upper flask body 3 is provided with bottle lid 4, and the bottom of well culture room 1 is provided with down culture room 6.

As shown in fig. 2, the both ends of well culture room 1 all are provided with sealed screw thread 5, and the both ends of well culture room 1 are passed through sealed screw thread 5 and are connected in culture room 6 and last culture room 2 down respectively, culture room 1 is connected with the lower part in the sealed screw thread 5 convenience that sets up through both ends, the convenient leakproofness that increases the connector when connecting, increase the length of blake bottle and the quantity of cultivateing the room, sealed screw thread 5 through setting up conveniently increases and reduces the blake room according to the needs of cultivateing and experiment, conveniently control the length of blake bottle at any time, reduce the unstability that the bottle caused by the blake bottle height.

As shown in fig. 3, the fixed increase of the bottom of lower culture room 6 grinds convex body 7 that is provided with, increase the frictional force that grinds convex body 7 and conveniently increase the bottom for the blake bottle when using through the setting of 6 bottoms in lower culture room, increase the blake bottle bottom and the contact dynamics of desktop, the convenient probability that takes place to empty that reduces when the blake bottle is placed, the convenient influence that reduces the blake bottle and empty and cause internal cell culture when cultivateing, the damage of cell when conveniently reducing the blake bottle, reduce the blake bottle and receive the collision and cause the probability of damage, increase the stability of blake bottle.

As shown in fig. 4, the bottom of culture room 2 has been seted up threaded seal mouth 8, culture inner chamber 9 has been seted up to the inside of going up culture room 2, the threaded seal mouth 8 that sets up through going up culture room 2 bottom is convenient to be connected well culture room 1 and last culture room 2 with culture inner chamber 9, the cell of cultivateing in the blake bottle can be increased to the mode through threaded connection, conveniently cultivate different cell and the same cell, increase the quantity of blake room in the blake bottle, conveniently carry out the multicellular culture in a blake bottle, conveniently contrast when cultivateing, reduce the use of blake bottle, the length of threaded seal mouth 8 is greater than sealing thread 5, conveniently seal the screw mouth, reduce the influence that outside gas caused to the cultivation of inside.

As shown in fig. 5, go up bottle 3's inside and be provided with interior wall 10 of washing, interior wall 10 through setting up in last bottle 3 is convenient when cultivateing the cell, can reduce the clearance dead angle in the culture room, increase and can wash for the culture room after accomplishing once to cultivate in the culture room, reduce the influence that the dead angle in the culture room caused the remaining of cell to cause the experiment, it piles up to reduce the cell of the indoor dead angle department of culture, it has the dead angle of clearance to reduce the application of sample rifle, conveniently increase the application of sample rifle and carry out abluent cleanliness factor for culture room inside, conveniently increase the accuracy of experiment next time, make things convenient for the application of sample rifle for the angle of culture room clearance.

The synthesis method comprises the following steps: the inner clear wall 10 arranged in the upper bottle body 3 is convenient for reducing the cleaning dead angle in the culture chamber when culturing cells, the culture chamber can be cleaned comprehensively after finishing one-time culture in the culture chamber, the influence of cell residue caused by the dead angle in the culture chamber on an experiment is reduced, the cell accumulation at the dead angle in the culture chamber is reduced, the dead angle of cleaning a sample adding gun is reduced, the cleanliness of cleaning the interior of the culture chamber by the sample adding gun is increased, the accuracy of the next experiment is increased, the angle of cleaning the culture chamber by the sample adding gun is facilitated, the friction force of the bottom of the culture bottle is increased when the culture bottle is used by the polishing convex body 7 arranged at the bottom of the lower culture chamber 6, the contact force between the bottom of the culture bottle and a table top is increased, the probability of toppling when the culture bottle is placed is reduced, and the influence of toppling of the culture bottle on the culture of the inner cells is reduced when culturing is facilitated, the damage of cell when convenient reduction cultivates in the blake bottle, it causes the probability of damage to reduce the blake bottle to receive the collision, increase the stability of blake bottle, the sealed mouth of screw 8 and the culture inner chamber 9 that set up through 2 bottoms of last blake bottle are convenient to be connected well blake chamber 1 and last blake chamber 2, the cell of cultivateing in can increasing the blake bottle through threaded connection's mode, conveniently cultivate different cell and the same cell, increase the quantity of blake chamber in the blake bottle, conveniently carry out the cell culture in a blake bottle, conveniently contrast when cultivateing, reduce the use of blake bottle, the length of sealed mouth of screw 8 is greater than sealing thread 5, conveniently seal the screw, reduce the influence that outside gas caused to the cultivation of inside.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A novel cell culture bottle improves device, includes well culture room (1), its characterized in that: the top of well cultivation room (1) is provided with upper culture room (2), the top of upper culture room (2) is fixed and is provided with upper bottle (3), the top of upper bottle (3) is provided with bottle lid (4), the bottom of well cultivation room (1) is provided with down cultivates room (6).

2. The novel cell culture flask improvement device of claim 1, characterized in that: the two ends of the middle culture chamber (1) are provided with sealing threads (5), and the two ends of the middle culture chamber (1) are respectively connected with the lower culture chamber (6) and the upper culture chamber (2) through the sealing threads (5).

3. The novel cell culture flask improvement device of claim 1, characterized in that: and the bottom of the lower culture chamber (6) is fixedly provided with an abrasion increasing convex body (7).

4. The novel cell culture flask improvement device of claim 1, characterized in that: the bottom of the upper culture chamber (2) is provided with a thread sealing port (8), and the inside of the upper culture chamber (2) is provided with a culture inner cavity (9).

5. The novel cell culture flask improvement device of claim 1, characterized in that: an inner washing wall (10) is arranged in the upper bottle body (3).