CN201873682U - Bacteria culturing bottle - Google Patents
Bacteria culturing bottle Download PDFInfo
- Publication number
- CN201873682U CN201873682U CN 201020242276 CN201020242276U CN201873682U CN 201873682 U CN201873682 U CN 201873682U CN 201020242276 CN201020242276 CN 201020242276 CN 201020242276 U CN201020242276 U CN 201020242276U CN 201873682 U CN201873682 U CN 201873682U
- Authority
- CN
- China
- Prior art keywords
- bottle
- bacteria
- culturing
- culturing bottle
- projection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a bacteria culturing bottle, which is characterized in that 1 to 4 inward bulges are arranged at the edge of the bottom part of a conical bottle body. During the shaking process, the bulges at the bottom of the bottle can play the role of mixing, so that culture solution can be uniformly distributed at the lower half part of the bottle effectively, and bacteria and the culture solution can be prevented from adhering to the wall of the bottle. The bacteria culturing bottle can help bacterial cells obtain sufficient oxygen, so as to ensure that the bacterial cells can reproduce in a good condition; therefore, the density of cultured bacteria is improved, so that the output of recombinant proteins is improved; and deviation and failure of culture results due to the external causes can be prevented.
Description
Technical field
The utility model relates to a kind of at culturing bottle, relates in particular to a kind of bacteria culture bottle that uses in the microbial culture technical field.
Background technology
When the Erlenmeyer flask of the present bacteria culture bottle that uses as glass material, as shown in Figure 1, existing culturing bottle bottle wall is smooth, and lines are soft.Use such culturing bottle, inoculum is deposited on culturing bottle bottom, during rocking, can occur inhomogeneous, adhere to phenomenon such as bottle wall, cause bacterial cell can't obtain equalization, sufficient oxygen.These bring difficulty for microbial culture work, cause the deviation of experimental result.
The purpose of this utility model is exactly that a kind of novel culturing bottle of design overcomes above defective, eliminate as much as possible because rock inhomogeneous and the detrimentally affect to microbial culture work that cause, make bacterial cell can fully obtain oxygen, breed well and grow, improve microbial culture density; And the manufacture craft of this type of culturing bottle is simple, production cost is low, easily large-scale popularization.
Summary of the invention
The purpose of this utility model provides a kind of bacteria culture bottle, inoculum can fully be rocked and suffered dynamics evenly, does not stick on bottle wall, make cell obtain impartial, oxygen fully, thereby reach bacterium surviving rate height, effect that culture density is big; Compare with traditional bacteria culture bottle, under equal conditions, recombinant exogenous protein matter output can improve greatly; And the manufacture craft of culturing bottle is simple, production cost is low, is beneficial to large-scale popularization.
The bacteria culture bottle that the utility model provides, bottle wall are provided with at least one inside projection.
The bacteria culture bottle that the utility model provides, described bossing are arranged on the described body circumference.
The bacteria culture bottle that the utility model provides, described bossing also can be arranged on described bottle feather edge.
The bacteria culture bottle that the utility model provides, described projection be shaped as taper.
The bacteria culture bottle that the utility model provides, circumference evenly is provided with 1～4 projection in bottom, its bottle wall upper edge.
The bacteria culture bottle that the utility model provides, the distance of the vertex of a cone of described projection and bottom bottle wall is 1.0～2.0cm.
Owing on the bottle wall of bacteria culture bottle, be provided with a plurality of projectioies, the utility model bacteria culture bottle is compared with the common bacteria culturing bottle, this projection has played the effect of a stirring rod, in the constant temperature shaking table during culturing bacterium, along with rocking of culturing bottle, inoculum is carried out mild stirring, thereby make bacterium fully obtain oxygen, breed well and grow.To bacterium high-density culture and expression foreign protein the time, can improve the foreign protein output of 10%-50%.Bacteria culture bottle is to be made by glass, only need do processing slightly in glass blowing, can finish the making of culturing bottle.Therefore, because the manufacture craft of culturing bottle is simple, production cost is low, is beneficial to large-scale popularization.
Description of drawings
Fig. 1 is the structure iron of existing culturing bottle;
Fig. 2 is the structure iron of culturing bottle of the present utility model.
Embodiment
Embodiment
As shown in Figure 2, be the structure iron of culturing bottle of the present utility model.Wherein, on the bottom on the bacteria culture bottle 12, be provided with 3 inwardly outstanding projectioies 3 equably.Described protruding 3 is conical in shape.The distance that projection 3 is inwardly outstanding is 1.0cm.
As described in Figure 2, the projection 3 of bacteria culture bottle 1 is arranged on the bottom 2, and this projection also can be arranged on the described body 4.
The shape of the projection 3 of the utility model bacteria culture bottle also can be needle-like or strip.Described projection can be provided with 3-4 equably, and 3 projectioies preferably are set.
Distance at the bottom of protruding 3 outstanding bottles is 1.0～2.0cm, preferably 1.0cm.
Manufacturing process can be by further processing acquisition to traditional culturing bottle.Local warming makes its deliquescing in traditional culturing bottle bottom, cuts fast in its edge, forms 3～4 inwardly protrudings that highly are about 1.0-2.0cm; Then, this bottle is placed homo genizing annelaing under the high temperature, promptly make bacteria culture bottle of the present utility model.
In addition, in rocking the process of nutrient solution, because the even stirring action of bottle end projection 3, can smash the bacterial cell that is sticked together, it is evenly distributed in the nutrient solution, thereby helps bacterium to obtain impartial, sufficient oxygen, the good breeding and the growth that keep bacterial cell.Simultaneously also can prevent that bacterial cell and nutrient solution from sticking on bottle wall, cause meaningless waste, reduce the influence of external cause to the full extent microbial culture work.
The foregoing description of the present utility model is not a limitation of the present invention.Therefore, under the spirit and scope that do not deviate from inventive concept, variation and advantage that those skilled in the art can expect all are included among the present invention, and are protection domain with the appending claims.
Claims (6)
1. a bacteria culture bottle is characterized in that, the bottle wall is provided with at least one inwardly outstanding projection.
2. culturing bottle as claimed in claim 1 is characterized in that, described bossing is arranged on the described body circumference.
3. culturing bottle as claimed in claim 1 is characterized in that, described bossing is arranged on described bottle feather edge.
4. culturing bottle as claimed in claim 1 is characterized in that, described projection be shaped as taper.
5. culturing bottle as claimed in claim 1 is characterized in that, circumference evenly is provided with 1～4 projection in bottle wall upper edge.
6. culturing bottle as claimed in claim 1 is characterized in that, the distance of the outstanding bottle of described projection wall is 1.0-2.0cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020242276 CN201873682U (en) | 2010-06-28 | 2010-06-28 | Bacteria culturing bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020242276 CN201873682U (en) | 2010-06-28 | 2010-06-28 | Bacteria culturing bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201873682U true CN201873682U (en) | 2011-06-22 |
Family
ID=44161608
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201020242276 Expired - Fee Related CN201873682U (en) | 2010-06-28 | 2010-06-28 | Bacteria culturing bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201873682U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102776116A (en) * | 2011-05-12 | 2012-11-14 | 千禾味业食品股份有限公司 | Aerobic fermentation container |
| CN113528344A (en) * | 2021-07-22 | 2021-10-22 | 青岛思拓新源细胞医学有限公司 | Umbilical cord tissue mesenchymal stem cell culture assembly and use method thereof |
-
2010
- 2010-06-28 CN CN 201020242276 patent/CN201873682U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102776116A (en) * | 2011-05-12 | 2012-11-14 | 千禾味业食品股份有限公司 | Aerobic fermentation container |
| CN113528344A (en) * | 2021-07-22 | 2021-10-22 | 青岛思拓新源细胞医学有限公司 | Umbilical cord tissue mesenchymal stem cell culture assembly and use method thereof |
| CN113528344B (en) * | 2021-07-22 | 2023-12-01 | 青岛思拓新源细胞医学有限公司 | Umbilical cord tissue mesenchymal stem cell culture assembly and use method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104630138B (en) | A kind of serum-free cultured chondrocytes liquid | |
| CN105238741A (en) | Complete medium for culturing ruminal epithelium cells of dairy cows and application thereof | |
| CN111334407B (en) | Process technology and process equipment for preparing yeast culture by deep fermentation of yeast | |
| CN201873682U (en) | Bacteria culturing bottle | |
| CN204689916U (en) | A kind of oxygenation ferment device of bisporous mushroom culture material | |
| CN203284404U (en) | Bacterial culture vessel | |
| CN104164365B (en) | A kind of cell in vitro contact co-culture device and cultivation operational approach thereof | |
| CN205676484U (en) | Egg mother cell and embryo transporting device | |
| CN203668367U (en) | Microalgae photobioreactor | |
| CN205999369U (en) | Culture dish placing box is used in a kind of experiment | |
| CN108384748A (en) | A method of automation culture diploid cell | |
| CN203333659U (en) | Bacteria culture bottle conducive to growth of bacteria | |
| CN109022290A (en) | A kind of wild rice smut haploid strains UeMTSP and its application | |
| CN104830752A (en) | Single-cell cultivation method of porcine embryonic fibroblasts | |
| CN204138673U (en) | A kind of culture dish and culturing bottle dual-purpose apparatus | |
| CN203072564U (en) | Hyacinth water curing container | |
| CN105296407A (en) | Method for culturing avibacterium paragallinarum bacterial solution | |
| CN204607986U (en) | A kind of novel fermentation storehouse sieve apparatus | |
| CN101819446B (en) | Method for controlling fungus culturing flow environment during edible fungus industrial cultivation | |
| CN202415564U (en) | Double-sided cell culture bottle | |
| CN104946586A (en) | Pretreatment method of mesenchymal stem cells and preparation obtained from mesenchymal stem cells | |
| CN103289949A (en) | Cell culture method direct from Kolle flask to bioreactor | |
| CN203462039U (en) | Suspension type continuous pipeline culture device for photosynthetic bacteria | |
| CN109136099A (en) | A kind of wild rice smut haploid strains UeTSP and its application | |
| CN202786260U (en) | Mammalian embryo swing incubator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110622 Termination date: 20180628 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |